ID A0A091UJQ6_PHORB Unreviewed; 393 AA. AC A0A091UJQ6; DT 26-NOV-2014, integrated into UniProtKB/TrEMBL. DT 26-NOV-2014, sequence version 1. DT 27-NOV-2024, entry version 21. DE RecName: Full=Macrophage-expressed gene 1 protein {ECO:0000256|ARBA:ARBA00021365}; DE AltName: Full=Perforin-2 {ECO:0000256|ARBA:ARBA00030728}; DE Flags: Fragment; GN ORFNames=N337_11537 {ECO:0000313|EMBL:KFQ90412.1}; OS Phoenicopterus ruber ruber. OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; OC Coelurosauria; Aves; Neognathae; Neoaves; Mirandornithes; OC Phoenicopteriformes; Phoenicopteridae; Phoenicopterus. OX NCBI_TaxID=9218 {ECO:0000313|EMBL:KFQ90412.1, ECO:0000313|Proteomes:UP000053700}; RN [1] {ECO:0000313|EMBL:KFQ90412.1, ECO:0000313|Proteomes:UP000053700} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=BGI_N337 {ECO:0000313|EMBL:KFQ90412.1}; RA Zhang G., Li C.; RT "Genome evolution of avian class."; RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Pore-forming protein involved in both innate and adaptive CC immunity. Plays a central role in antigen cross-presentation in CC dendritic cells by forming a pore in antigen-containing compartments, CC thereby promoting delivery of antigens for cross-presentation. Also CC involved in innate immune response following bacterial infection; shows CC antibacterial activity against a wide spectrum of Gram-positive, Gram- CC negative and acid-fast bacteria. Reduces the viability of the CC intracytosolic pathogen L.monocytogenes by inhibiting acidification of CC the phagocytic vacuole of host cells which restricts bacterial CC translocation from the vacuole to the cytosol. Required for the CC antibacterial activity of reactive oxygen species and nitric oxide. CC {ECO:0000256|ARBA:ARBA00045689}. CC -!- FUNCTION: Pore-forming protein that plays a central role in antigen CC cross-presentation in dendritic cells by mediating delivery of antigens CC for cross-presentation. Dendritic cells bridge innate and adaptive CC immunity by capturing exogenous antigens on MHC class-I molecules and CC presenting them to naive CD8(+) T-cells. Acts by forming a pore in CC antigen-containing compartments, promoting the release of antigens into CC the cytosol, enabling generation of MHCI:peptide complexes and T-cell CC priming. {ECO:0000256|ARBA:ARBA00045657}. CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome membrane CC {ECO:0000256|ARBA:ARBA00004265}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004265}. CC -!- SIMILARITY: Belongs to the MPEG1 family. CC {ECO:0000256|ARBA:ARBA00007256}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KK436749; KFQ90412.1; -; Genomic_DNA. DR AlphaFoldDB; A0A091UJQ6; -. DR Proteomes; UP000053700; Unassembled WGS sequence. DR GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell. DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW. DR GO; GO:0042742; P:defense response to bacterium; IEA:TreeGrafter. DR InterPro; IPR020864; MACPF. DR InterPro; IPR039707; MPEG1. DR PANTHER; PTHR31463; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1. DR PANTHER; PTHR31463:SF4; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1. DR Pfam; PF01823; MACPF; 1. DR SMART; SM00457; MACPF; 1. DR PROSITE; PS51412; MACPF_2; 1. PE 3: Inferred from homology; KW Adaptive immunity {ECO:0000256|ARBA:ARBA00023130}; KW Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Immunity {ECO:0000256|ARBA:ARBA00022859}; KW Innate immunity {ECO:0000256|ARBA:ARBA00022588}; KW Membrane {ECO:0000256|ARBA:ARBA00022989}; KW Reference proteome {ECO:0000313|Proteomes:UP000053700}; KW Signal {ECO:0000256|ARBA:ARBA00022729}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692}; KW Transmembrane beta strand {ECO:0000256|ARBA:ARBA00022452}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}; KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}. FT DOMAIN 1..300 FT /note="MACPF" FT /evidence="ECO:0000259|PROSITE:PS51412" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:KFQ90412.1" FT NON_TER 393 FT /evidence="ECO:0000313|EMBL:KFQ90412.1" SQ SEQUENCE 393 AA; 43531 MW; 232F2C46C511A55D CRC64; LPGGGWDNLR NLDMGRVINL GYSLCKTTED GSYIIPDEIF TIPRKQSNLD INSEIIESWK DYQSITSASI NLELSLFSAI NGKFSYDFHR TKTHQVKDRA VTTRVQVRNL VYTAKIDPGA VLDKSFKKQL LLIASHLENN QTRMADFLAE VLVLNYGTHT ITSVDAGASL VQEDQIKATF LKDSLATRTA VTTSAGVAFH SIIGVKNEES LDVSSGFTKQ YMENRTNSRV ESIGGTPFYP GITLKTWQEG IRNQLVALDR SGLPLYFFIN PGTLPELPTP GPPRRYYTFN TYLGCTDATS PNFNFHANAD DGSCEGAMTN FTFGGVFQEC AGLAGPDTSA LCRELEQRNP LTGAFSCPPT YTPVLLGVQE REEGHSHLEC HDKCTLRIFC HRE //