ID   A0A085PBE7_ECOLX        Unreviewed;       455 AA.
AC   A0A085PBE7;
DT   29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   29-OCT-2014, sequence version 1.
DT   07-SEP-2016, entry version 12.
DE   RecName: Full=Replicative DNA helicase {ECO:0000256|RuleBase:RU362085};
DE            EC=3.6.4.12 {ECO:0000256|RuleBase:RU362085};
GN   ORFNames=JD73_08065 {ECO:0000313|EMBL:KFD79043.1};
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562 {ECO:0000313|EMBL:KFD79043.1, ECO:0000313|Proteomes:UP000028651};
RN   [1] {ECO:0000313|EMBL:KFD79043.1, ECO:0000313|Proteomes:UP000028651}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=48 {ECO:0000313|EMBL:KFD79043.1,
RC   ECO:0000313|Proteomes:UP000028651};
RA   Nuesch-Inderbinen M.T., Funk J., Cernela N., Tasara T., Klumpp J.,
RA   Schmidt H., Stephan R.;
RT   "Prevalence of subtilase cytotoxin-encoding subAB variants among Shiga
RT   toxin-producing Escherichia coli strains isolated from wild ruminants
RT   and sheep differs from that of cattle and pigs and is predominated by
RT   the new allelic variant subAB2-2.";
RL   Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Participates in initiation and elongation during
CC       chromosome replication; it exhibits DNA-dependent ATPase activity
CC       and contains distinct active sites for ATP binding, DNA binding,
CC       and interaction with DnaC protein, primase, and other prepriming
CC       proteins. {ECO:0000256|RuleBase:RU362085}.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC       {ECO:0000256|RuleBase:RU362085}.
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000256|RuleBase:RU362085}.
CC   -!- SIMILARITY: Contains 1 SF4 helicase domain.
CC       {ECO:0000256|RuleBase:RU362085}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:KFD79043.1}.
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DR   EMBL; JPQG01000001; KFD79043.1; -; Genomic_DNA.
DR   RefSeq; WP_044187700.1; NZ_JPQG01000001.1.
DR   EnsemblBacteria; KFD79043; KFD79043; JD73_08065.
DR   Proteomes; UP000028651; Unassembled WGS sequence.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|RuleBase:RU362085};
KW   Complete proteome {ECO:0000313|Proteomes:UP000028651};
KW   DNA replication {ECO:0000256|RuleBase:RU362085,
KW   ECO:0000256|SAAS:SAAS00435671};
KW   DNA-binding {ECO:0000256|RuleBase:RU362085,
KW   ECO:0000256|SAAS:SAAS00435659};
KW   Helicase {ECO:0000256|RuleBase:RU362085, ECO:0000313|EMBL:KFD79043.1};
KW   Hydrolase {ECO:0000256|RuleBase:RU362085};
KW   Nucleotide-binding {ECO:0000256|RuleBase:RU362085};
KW   Primosome {ECO:0000256|RuleBase:RU362085}.
FT   DOMAIN      176    450       SF4 helicase. {ECO:0000259|PROSITE:
FT                                PS51199}.
SQ   SEQUENCE   455 AA;  49646 MW;  05D079F7E9F07999 CRC64;
     MAEMSVLCAY EAEQAVLGGL MLENERWDEV VLMLSPDDFY VAQHRAIFRA MSELAVNGQP
     LDLITLSEHL EHQGRLDVLG GFAYLAELSK NTPSAANIMA YAGIVVEKSV LRQLQATGNT
     LIADVVAPNA TSRDVLDAAE RRLFALAQNR TLRERTEASL SSALDTVLQQ LESVLGSGGV
     TGTPTGFDCL DEMTCGLQPG DLVLLAARPS MGKTSLALSM CLSAALRRTE ESVLVFSIEM
     PKGQLILRML SMLGRVELNH LRSGNMDDED WLRVSSAVGM ALGDGGMGSL GERIIIDDCC
     EQTPSSLRAG ARRYTRLYGK PSLIMVDYLQ LIRAPELENR TQEIAEISRS LKALGKELGC
     PVLALSQLNR QVEQRADKRP NNGDLRDSGA LEQDADLIMF IYRDEVYNPD SLAKGEAEII
     VSKQRQGPTG TVRVRFDGRY TLFTPSENDG PGGYA
//