ID A0A085AC20_9ENTR Unreviewed; 820 AA. AC A0A085AC20; DT 29-OCT-2014, integrated into UniProtKB/TrEMBL. DT 29-OCT-2014, sequence version 1. DT 31-JAN-2018, entry version 20. DE RecName: Full=Bifunctional aspartokinase/homoserine dehydrogenase {ECO:0000256|PIRNR:PIRNR000727}; DE Includes: DE RecName: Full=Aspartokinase {ECO:0000256|PIRNR:PIRNR000727}; DE EC=2.7.2.4 {ECO:0000256|PIRNR:PIRNR000727}; DE Includes: DE RecName: Full=Homoserine dehydrogenase {ECO:0000256|PIRNR:PIRNR000727}; DE EC=1.1.1.3 {ECO:0000256|PIRNR:PIRNR000727}; GN Name=thrA {ECO:0000313|EMBL:KFC07765.1}; GN ORFNames=GTGU_01726 {ECO:0000313|EMBL:KFC07765.1}; OS Trabulsiella guamensis ATCC 49490. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Trabulsiella. OX NCBI_TaxID=1005994 {ECO:0000313|EMBL:KFC07765.1, ECO:0000313|Proteomes:UP000028630}; RN [1] {ECO:0000313|EMBL:KFC07765.1, ECO:0000313|Proteomes:UP000028630} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 49490 {ECO:0000313|EMBL:KFC07765.1, RC ECO:0000313|Proteomes:UP000028630}; RA Plunkett G.III., Neeno-Eckwall E.C., Glasner J.D., Perna N.T.; RT "ATOL: Assembling a taxonomically balanced genome-scale reconstruction RT of the evolutionary history of the Enterobacteriaceae."; RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: ATP + L-aspartate = ADP + 4-phospho-L- CC aspartate. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- CATALYTIC ACTIVITY: L-homoserine + NAD(P)(+) = L-aspartate 4- CC semialdehyde + NAD(P)H. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP CC pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de CC novo pathway; L-homoserine from L-aspartate: step 1/3. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de CC novo pathway; L-homoserine from L-aspartate: step 3/3. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L- CC threonine from L-aspartate: step 1/5. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L- CC threonine from L-aspartate: step 3/5. CC {ECO:0000256|PIRNR:PIRNR000727}. CC -!- SUBUNIT: Homotetramer. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- SIMILARITY: In the C-terminal section; belongs to the homoserine CC dehydrogenase family. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- SIMILARITY: In the N-terminal section; belongs to the CC aspartokinase family. {ECO:0000256|PIRNR:PIRNR000727}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:KFC07765.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JMTB01000059; KFC07765.1; -; Genomic_DNA. DR RefSeq; WP_038155763.1; NZ_JMTB01000059.1. DR EnsemblBacteria; KFC07765; KFC07765; GTGU_01726. DR UniPathway; UPA00034; UER00015. DR UniPathway; UPA00050; UER00063. DR UniPathway; UPA00051; UER00462. DR Proteomes; UP000028630; Unassembled WGS sequence. DR GO; GO:0004072; F:aspartate kinase activity; IEA:UniProtKB-EC. DR GO; GO:0004412; F:homoserine dehydrogenase activity; IEA:UniProtKB-EC. DR GO; GO:0050661; F:NADP binding; IEA:InterPro. DR GO; GO:0009067; P:aspartate family amino acid biosynthetic process; IEA:InterPro. DR Gene3D; 3.40.1160.10; -; 1. DR InterPro; IPR036393; AceGlu_kinase-like_sf. DR InterPro; IPR002912; ACT_dom. DR InterPro; IPR001048; Asp/Glu/Uridylate_kinase. DR InterPro; IPR005106; Asp/hSer_DH_NAD-bd. DR InterPro; IPR001341; Asp_kinase. DR InterPro; IPR018042; Aspartate_kinase_CS. DR InterPro; IPR011147; Bifunc_aspartokin/hSer_DH. DR InterPro; IPR027795; GATS-like_ACT_dom. DR InterPro; IPR001342; HDH_cat. DR InterPro; IPR019811; HDH_CS. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR Pfam; PF00696; AA_kinase; 1. DR Pfam; PF01842; ACT; 1. DR Pfam; PF13840; ACT_7; 1. DR Pfam; PF00742; Homoserine_dh; 1. DR Pfam; PF03447; NAD_binding_3; 1. DR PIRSF; PIRSF000727; ThrA; 1. DR SUPFAM; SSF51735; SSF51735; 1. DR SUPFAM; SSF53633; SSF53633; 1. DR TIGRFAMs; TIGR00657; asp_kinases; 1. DR PROSITE; PS51671; ACT; 2. DR PROSITE; PS00324; ASPARTOKINASE; 1. DR PROSITE; PS01042; HOMOSER_DHGENASE; 1. PE 3: Inferred from homology; KW Amino-acid biosynthesis {ECO:0000256|PIRNR:PIRNR000727}; KW ATP-binding {ECO:0000256|PIRNR:PIRNR000727}; KW Complete proteome {ECO:0000313|Proteomes:UP000028630}; KW Kinase {ECO:0000256|PIRNR:PIRNR000727, ECO:0000313|EMBL:KFC07765.1}; KW NADP {ECO:0000256|PIRNR:PIRNR000727}; KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000727}; KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR000727, KW ECO:0000313|EMBL:KFC07765.1}; KW Reference proteome {ECO:0000313|Proteomes:UP000028630}; KW Transferase {ECO:0000256|PIRNR:PIRNR000727, KW ECO:0000313|EMBL:KFC07765.1}. FT DOMAIN 320 394 ACT. {ECO:0000259|PROSITE:PS51671}. FT DOMAIN 401 478 ACT. {ECO:0000259|PROSITE:PS51671}. SQ SEQUENCE 820 AA; 88559 MW; FA495C59C436B101 CRC64; MRVLKFGGTS VANAERFLRV ADILESNARQ GQVATVLSAP AKITNHLVAM IEKTIGGQDA LTNISDAERI FAELLQGLAD AQPGFPLAQL KLFVEQEFAQ IKHVLHGISL LGQCPDSVNA AIICRGEKLS IAIMAGLLEA RGHNVTVIDP VEKLLAVGHY LESTVDIAES TRRIAASQIP ADHMVLMAGF TAGNEKGELV VLGRNGSDYS AAVLAACLRA DCCEIWTDVD GVYTCDPRQV PDARLLKSMS YQEAMELSYF GAKVLHPRTI APIAQFQIPC LIKNTGNPQA PGTLIGASRD EDGLPVKGIS NLNNMAMFNV SGPGMKGMVG MAARVFATMS RARISVVLIT QSSSEYSISF CVPQADCARA KKAMEDEFYL ELKEGVLEPL SIMERLAIIS VVGDGMRTLR GISAKFFAAL ARANINIVAI AQGSSERSIS VVVNNDDATT GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL LEQLKRQQSW LKSKHIDLRV CGVANSKALL TSVHGLNLEN WKGALAEAKE PFNLGRLIRL VKEYHLLNPV IVDCTSSQAV ADQYADFLRE GFHVVTPNKK ANTSSMNYYH ELRQAAAGSR RKFLYDTNVG AGLPVIENLQ NLLNAGDELQ RFSGILSGSL SFIFGKLDEG MSLSAATTLA REMGYTEPDP RDDLSGMDVA RKLLILARET GRQLELSDIV VEPVLPEGFD VAGDVDSFMS RLPSLDAAFA ARIAKARDAG KVLRYVGNIE EDGVCRVKIA EVDGNDPLYK VKNGENALAF YSHYYQPLPL ILRGYGAGND VTAAGVFADL LRTLSWKLGV //