ID A0A075EC34_9VIRU Unreviewed; 2183 AA. AC A0A075EC34; DT 29-OCT-2014, integrated into UniProtKB/TrEMBL. DT 29-OCT-2014, sequence version 1. DT 22-FEB-2023, entry version 41. DE SubName: Full=RNA-dependent RNA polymerase {ECO:0000313|EMBL:AID68938.1}; OS Apple stem pitting virus. OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; OC Tymovirales; Betaflexiviridae; Quinvirinae; Foveavirus. OX NCBI_TaxID=35350 {ECO:0000313|EMBL:AID68938.1}; RN [1] {ECO:0000313|EMBL:AID68938.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=PB66 {ECO:0000313|EMBL:AID68938.1}; RA Arntjen A., Jelkmann W.; RT "Sequence variability of Apple stem pitting virus (ASPV) and virus RT occurence in apple trees throughout the year."; RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; CC Evidence={ECO:0000256|ARBA:ARBA00001556}; CC -!- SIMILARITY: Belongs to the potexviruses/carlaviruses RNA replication CC protein family. {ECO:0000256|ARBA:ARBA00008513}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KF321966; AID68938.1; -; Genomic_RNA. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016817; F:hydrolase activity, acting on acid anhydrides; IEA:InterPro. DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:UniProtKB-UniRule. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC. DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro. DR GO; GO:0006396; P:RNA processing; IEA:InterPro. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR Gene3D; 2.60.120.590; Alpha-ketoglutarate-dependent dioxygenase AlkB-like; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR037151; AlkB-like_sf. DR InterPro; IPR002588; Alphavirus-like_MT_dom. DR InterPro; IPR043502; DNA/RNA_pol_sf. DR InterPro; IPR003323; OTU_dom. DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR008041; Peptidase_C23. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol. DR Pfam; PF05379; Peptidase_C23; 1. DR Pfam; PF00978; RdRP_2; 1. DR Pfam; PF01443; Viral_helicase1; 1. DR Pfam; PF01660; Vmethyltransf; 1. DR SUPFAM; SSF51197; Clavaminate synthase-like; 1. DR SUPFAM; SSF56672; DNA/RNA polymerases; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR PROSITE; PS51743; ALPHAVIRUS_MT; 1. DR PROSITE; PS51471; FE2OG_OXY; 1. DR PROSITE; PS50802; OTU; 1. DR PROSITE; PS51492; PEPTIDASE_C23; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840}; KW Helicase {ECO:0000256|ARBA:ARBA00022806}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022806}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484, KW ECO:0000313|EMBL:AID68938.1}; Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}. FT DOMAIN 63..256 FT /note="Alphavirus-like MT" FT /evidence="ECO:0000259|PROSITE:PS51743" FT DOMAIN 762..853 FT /note="Fe2OG dioxygenase" FT /evidence="ECO:0000259|PROSITE:PS51471" FT DOMAIN 1091..1199 FT /note="OTU" FT /evidence="ECO:0000259|PROSITE:PS50802" FT DOMAIN 1198..1288 FT /note="Peptidase C23" FT /evidence="ECO:0000259|PROSITE:PS51492" FT DOMAIN 1340..1667 FT /note="(+)RNA virus helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51657" FT DOMAIN 1961..2068 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50507" FT REGION 886..907 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 2183 AA; 246810 MW; 2C1D389E2BCC6D09 CRC64; MALLSRTAAE EVIASFTSEE QSRISTQAVL ALTNVEKDKH DLFNYALPEL AKMKLFKSGI YLSPHSYRPH SHPVCKTLEN NILFNILPSY LDNSFYLVSI KKNKVDFLKR RHPDLQMVET INRYISSIDK TRYGGFFHVS PSKISAKFKC DRRTGFENDA SLVDLIPGCM EGARKRFFFH DELHYWTKEA LITFLDHVRP EVMLASIVFP PEILAGAKES LNPWCYTFKI LGKDLVFFPD GEQSEAYIQP VSGSYLLKTG KITTPSGDVF QLDLLKSSFS HHLISITKGE AIGQKMRFFN GFEAVAMKGL NPLRRKVESC LPISKSTILK IYRYLRTLKK PDLQSAMAKL SQVCKDPNGY EIKFFEEFSK LCLKCETLNT NMLPDMKRIV QGFFLKLFPN PIARNFKVVQ QLHLDNFIET LEEFNFSIST EDLSLNWKDD LEFVNLTFGD TDFNMEESFA SVWGTQKDVV DITTVHHSPY MVSKFDSYDH QFHSLLSVNS TTALTKIAKI VLSLYDPCVV GAFSECRVSN LAVNVIIAAN LRACFAVTDL WQTFEGILLR ECKKAQGKMR KKFYFELGIK WFLFVDVSNQ WFLPPCRDGL ISRSVLFDQF IKGCQRHNSF HNGRMALRQV FRNFKLQALL DESELSIIHM VEIGKASEVK PAAENEQNLN SISPEIIPIT TVGCNVVPCK CNLNCFIQQA DVSSLHGNLV FLDFIGGARG RGASFYSRDF KGYSYTGFSH VSRGWPDFLD KFLSDNKIPQ KFYNQCLIQE YSTGHGLSMH KDDEGIYDIN HQVLTVNYSG DAIFCIECSG SGFEVPLSGP QMLLMPFGFQ REHRHGIKSP SKGRISLTFR LAKEDDHQVP VQEVVAIEHG DSDDRAALKQ METECRLHGG KPSSDSGGQN REKVTGDPCG SAPVQEFLIQ IDSSILEYAL KSLSGLSKDV VNCDMCLCNS PWLKNEELKF SEALRDLAFA SGFNPTDRFS LAKGIEGVRG MNRIINELPT HVFPLRGTMH VVDLDDKSIK GDIKEGFFSG FRRWKITSCS TDLIVLAFLK PKMTLGGELR SHEDECELSD LTEKLHGCSV ILSRKFEPDL FHSFDVEADG NCFWHSVGPL IGVDGEYLKK ILHDQALKDG VKCPRLSKQL EGSTWAEREA VAYFCSHYGI RLNVLYTREE CTWIFKPHEV LKAATLVCQD NHFKPCMPVN GCVIRAIASA LNRREVDVLA VLGKPAHEDL FEEVAEGRGF SIFDLTRLFE IFSICGSVDT GGELIMVNEN GKIPAEFSLE KEHLAHIPTL SRRKFSPIVS DLNRVSNSAM RFLAINGAEI DYRPSIDRAS TLLDSFELGA TGVLCQGIKE AQKGLAAKLV PESVHDRKLI MILGTFGCGK SSLFKKFIEK SPGKAITFVS PRRSLAESIN HDLGLARVGG KKAGKCKDLK NVRVKTFELF ILHLDSIKEG HTVVIDEIQL FPPGYIDLII LGLNLKVNII IAGDPCQSDY DSSSDRHIFA GSESDIMRIL SGRSYKFNIL SQRFRNPVFH GRLPCNLNKT RLTLDEEEYT LWDSIQEFSM MGRKDCPVVL VSSFEEKKIV AAHLGLKMKC ITYGESTGLN FQKGAILVTY ESALTSDRRW WTALSRFSHD IHFINGMGVT WDNAITHFVG KPLHKFFTKR ACNDDIIDLL PGRPELIEGF QSQVGADEGV REAKLAGDPW LKTKIFLGQS ADVEPELLDE VEAAEDWFKT HIPIMGLEAV RAQWVDRLIS REEREFRIGD MTTEQFTDEH SKNRGQELTN AAERYEAIYP RHKGTDTATF LMAVKKRLSF SSPAAEHAKL RRARPFGKFL LNTFLKRVPL NSRHNEAMMS DAVHAFEEKK LSKSMATIEN HSGRSCEDWP VDKALIFMKS QLCTKFDNRF RSAKAGQTLA CFQHSVLCRF APYMRYIEAK VVEVLPKNLY IHSGKNIEDL AAWVTVNKFN GICTESDYEA FDASQDHFIL AFELEVMKFL GLPPDLIADY TFIKTHLGSK LGNFAIMRFT GEASTFLFNT MANMLFTFLR YDLNGKEAIC FAGDDMCANS RLKVTNVHSK FLDKIKLKAK VQFTATPTFC GWGLCEHGVF KKPDLVLERL QIARETRNLE NCIDNYAIEV SCAYKMGENL SLYLTPQEVD AHYNCVRFIV QHNHLLKSNI RDLFRGEASL SVN //