ID A0A075EC34_9VIRU Unreviewed; 2183 AA. AC A0A075EC34; DT 29-OCT-2014, integrated into UniProtKB/TrEMBL. DT 29-OCT-2014, sequence version 1. DT 04-FEB-2015, entry version 3. DE SubName: Full=RNA-dependent RNA polymerase {ECO:0000313|EMBL:AID68938.1}; OS Apple stem pitting virus. OC Viruses; ssRNA positive-strand viruses, no DNA stage; Tymovirales; OC Betaflexiviridae; Foveavirus. OX NCBI_TaxID=35350 {ECO:0000313|EMBL:AID68938.1}; RN [1] {ECO:0000313|EMBL:AID68938.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=PB66 {ECO:0000313|EMBL:AID68938.1}; RA Arntjen A., Jelkmann W.; RT "Sequence variability of Apple stem pitting virus (ASPV) and virus RT occurence in apple trees throughout the year."; RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate CC + RNA(n+1). {ECO:0000256|SAAS:SAAS00005403}. CC -!- SIMILARITY: Contains (+)RNA virus helicase ATP-binding domain. CC {ECO:0000256|SAAS:SAAS00005295}. CC -!- SIMILARITY: Contains (+)RNA virus helicase C-terminal domain. CC {ECO:0000256|SAAS:SAAS00005240}. CC -!- SIMILARITY: Contains RdRp catalytic domain. CC {ECO:0000256|SAAS:SAAS00005333}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KF321966; AID68938.1; -; Genomic_RNA. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0016817; F:hydrolase activity, acting on acid anhydrides; IEA:InterPro. DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro. DR GO; GO:0016706; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0006396; P:RNA processing; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR Gene3D; 2.60.120.590; -; 1. DR Gene3D; 3.40.50.300; -; 1. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR027450; AlkB-like. DR InterPro; IPR003323; OTU. DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR008041; Peptidase_C23. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR InterPro; IPR002588; Tymovirus_MeTrfase. DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol. DR Pfam; PF03171; 2OG-FeII_Oxy; 1. DR Pfam; PF02338; OTU; 1. DR Pfam; PF05379; Peptidase_C23; 1. DR Pfam; PF00978; RdRP_2; 1. DR Pfam; PF01443; Viral_helicase1; 1. DR Pfam; PF01660; Vmethyltransf; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS51471; FE2OG_OXY; 1. DR PROSITE; PS50802; OTU; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. PE 4: Predicted; KW Nucleotide-binding {ECO:0000256|SAAS:SAAS00005354}; KW Nucleotidyltransferase {ECO:0000256|SAAS:SAAS00005336}; KW RNA-directed RNA polymerase {ECO:0000256|SAAS:SAAS00005364, KW ECO:0000313|EMBL:AID68938.1}; KW Transferase {ECO:0000256|SAAS:SAAS00005346}; KW Viral RNA replication {ECO:0000256|SAAS:SAAS00005266}. SQ SEQUENCE 2183 AA; 246810 MW; 2C1D389E2BCC6D09 CRC64; MALLSRTAAE EVIASFTSEE QSRISTQAVL ALTNVEKDKH DLFNYALPEL AKMKLFKSGI YLSPHSYRPH SHPVCKTLEN NILFNILPSY LDNSFYLVSI KKNKVDFLKR RHPDLQMVET INRYISSIDK TRYGGFFHVS PSKISAKFKC DRRTGFENDA SLVDLIPGCM EGARKRFFFH DELHYWTKEA LITFLDHVRP EVMLASIVFP PEILAGAKES LNPWCYTFKI LGKDLVFFPD GEQSEAYIQP VSGSYLLKTG KITTPSGDVF QLDLLKSSFS HHLISITKGE AIGQKMRFFN GFEAVAMKGL NPLRRKVESC LPISKSTILK IYRYLRTLKK PDLQSAMAKL SQVCKDPNGY EIKFFEEFSK LCLKCETLNT NMLPDMKRIV QGFFLKLFPN PIARNFKVVQ QLHLDNFIET LEEFNFSIST EDLSLNWKDD LEFVNLTFGD TDFNMEESFA SVWGTQKDVV DITTVHHSPY MVSKFDSYDH QFHSLLSVNS TTALTKIAKI VLSLYDPCVV GAFSECRVSN LAVNVIIAAN LRACFAVTDL WQTFEGILLR ECKKAQGKMR KKFYFELGIK WFLFVDVSNQ WFLPPCRDGL ISRSVLFDQF IKGCQRHNSF HNGRMALRQV FRNFKLQALL DESELSIIHM VEIGKASEVK PAAENEQNLN SISPEIIPIT TVGCNVVPCK CNLNCFIQQA DVSSLHGNLV FLDFIGGARG RGASFYSRDF KGYSYTGFSH VSRGWPDFLD KFLSDNKIPQ KFYNQCLIQE YSTGHGLSMH KDDEGIYDIN HQVLTVNYSG DAIFCIECSG SGFEVPLSGP QMLLMPFGFQ REHRHGIKSP SKGRISLTFR LAKEDDHQVP VQEVVAIEHG DSDDRAALKQ METECRLHGG KPSSDSGGQN REKVTGDPCG SAPVQEFLIQ IDSSILEYAL KSLSGLSKDV VNCDMCLCNS PWLKNEELKF SEALRDLAFA SGFNPTDRFS LAKGIEGVRG MNRIINELPT HVFPLRGTMH VVDLDDKSIK GDIKEGFFSG FRRWKITSCS TDLIVLAFLK PKMTLGGELR SHEDECELSD LTEKLHGCSV ILSRKFEPDL FHSFDVEADG NCFWHSVGPL IGVDGEYLKK ILHDQALKDG VKCPRLSKQL EGSTWAEREA VAYFCSHYGI RLNVLYTREE CTWIFKPHEV LKAATLVCQD NHFKPCMPVN GCVIRAIASA LNRREVDVLA VLGKPAHEDL FEEVAEGRGF SIFDLTRLFE IFSICGSVDT GGELIMVNEN GKIPAEFSLE KEHLAHIPTL SRRKFSPIVS DLNRVSNSAM RFLAINGAEI DYRPSIDRAS TLLDSFELGA TGVLCQGIKE AQKGLAAKLV PESVHDRKLI MILGTFGCGK SSLFKKFIEK SPGKAITFVS PRRSLAESIN HDLGLARVGG KKAGKCKDLK NVRVKTFELF ILHLDSIKEG HTVVIDEIQL FPPGYIDLII LGLNLKVNII IAGDPCQSDY DSSSDRHIFA GSESDIMRIL SGRSYKFNIL SQRFRNPVFH GRLPCNLNKT RLTLDEEEYT LWDSIQEFSM MGRKDCPVVL VSSFEEKKIV AAHLGLKMKC ITYGESTGLN FQKGAILVTY ESALTSDRRW WTALSRFSHD IHFINGMGVT WDNAITHFVG KPLHKFFTKR ACNDDIIDLL PGRPELIEGF QSQVGADEGV REAKLAGDPW LKTKIFLGQS ADVEPELLDE VEAAEDWFKT HIPIMGLEAV RAQWVDRLIS REEREFRIGD MTTEQFTDEH SKNRGQELTN AAERYEAIYP RHKGTDTATF LMAVKKRLSF SSPAAEHAKL RRARPFGKFL LNTFLKRVPL NSRHNEAMMS DAVHAFEEKK LSKSMATIEN HSGRSCEDWP VDKALIFMKS QLCTKFDNRF RSAKAGQTLA CFQHSVLCRF APYMRYIEAK VVEVLPKNLY IHSGKNIEDL AAWVTVNKFN GICTESDYEA FDASQDHFIL AFELEVMKFL GLPPDLIADY TFIKTHLGSK LGNFAIMRFT GEASTFLFNT MANMLFTFLR YDLNGKEAIC FAGDDMCANS RLKVTNVHSK FLDKIKLKAK VQFTATPTFC GWGLCEHGVF KKPDLVLERL QIARETRNLE NCIDNYAIEV SCAYKMGENL SLYLTPQEVD AHYNCVRFIV QHNHLLKSNI RDLFRGEASL SVN //