ID A0A075BI22_9INFA Unreviewed; 757 AA. AC A0A075BI22; DT 29-OCT-2014, integrated into UniProtKB/TrEMBL. DT 04-FEB-2015, sequence version 2. DT 29-MAY-2024, entry version 45. DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|HAMAP-Rule:MF_04065}; DE EC=2.7.7.48 {ECO:0000256|HAMAP-Rule:MF_04065}; DE AltName: Full=Polymerase basic protein 1 {ECO:0000256|HAMAP-Rule:MF_04065}; DE Short=PB1 {ECO:0000256|HAMAP-Rule:MF_04065}; DE AltName: Full=RNA-directed RNA polymerase subunit P1 {ECO:0000256|HAMAP-Rule:MF_04065}; GN Name=PB1 {ECO:0000256|HAMAP-Rule:MF_04065, GN ECO:0000313|EMBL:AGK23693.2}; OS Influenza A virus (A/chicken/Shandong/11/2011(H9N2)). OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; OC Alphainfluenzavirus influenzae; Influenza A virus. OX NCBI_TaxID=1309781 {ECO:0000313|EMBL:AGK23693.2, ECO:0000313|Proteomes:UP000134749}; RN [1] {ECO:0000313|EMBL:AGK23693.2, ECO:0000313|Proteomes:UP000134749} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=A/chicken/Shandong/11/2011 {ECO:0000313|EMBL:AGK23693.2}; RX PubMed=25548189; DOI=10.1073/pnas.1422456112; RA Pu J., Wang S., Yin Y., Zhang G., Carter R.A., Wang J., Xu G., Sun H., RA Wang M., Wen C., Wei Y., Wang D., Zhu B., Lemmon G., Jiao Y., Duan S., RA Wang Q., Du Q., Sun M., Bao J., Sun Y., Zhao J., Zhang H., Wu G., Liu J., RA Webster R.G.; RT "Evolution of the H9N2 influenza genotype that facilitated the genesis of RT the novel H7N9 virus."; RL Proc. Natl. Acad. Sci. U.S.A. 112:548-553(2015). CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for CC replication and transcription of virus RNA segments. The transcription CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides CC by PA. In turn, these short capped RNAs are used as primers by PB1 for CC transcription of viral mRNAs. During virus replication, PB1 initiates CC RNA synthesis and copies vRNA into complementary RNA (cRNA) which in CC turn serves as a template for the production of more vRNAs. CC {ECO:0000256|HAMAP-Rule:MF_04065}. CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for CC replication and transcription of virus RNA segments. The transcription CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides CC by PA. In turn, these short capped RNAs are used as primers by PB1 for CC transcription of viral mRNAs. During virus replication, PB1 initiates CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn CC serves as a template for the production of more vRNAs. CC {ECO:0000256|ARBA:ARBA00002148}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.48; Evidence={ECO:0000256|HAMAP-Rule:MF_04065, CC ECO:0000256|RuleBase:RU004330}; CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1, CC PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). CC Interacts (via C-terminus) with PB2 (via N-terminus); this interaction CC is essential for transcription initiation. {ECO:0000256|HAMAP- CC Rule:MF_04065}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host CC nucleus {ECO:0000256|HAMAP-Rule:MF_04065}. Host cytoplasm CC {ECO:0000256|HAMAP-Rule:MF_04065}. Nucleus CC {ECO:0000256|ARBA:ARBA00004123}. CC -!- PTM: Phosphorylated by host PRKCA. {ECO:0000256|HAMAP-Rule:MF_04065}. CC -!- SIMILARITY: Belongs to the influenza viruses polymerase PB1 family. CC {ECO:0000256|HAMAP-Rule:MF_04065}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KC821097; AGK23693.2; -; Viral_cRNA. DR Proteomes; UP000134749; Genome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-UniRule. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule. DR GO; GO:0039523; P:symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-UniRule. DR GO; GO:0039694; P:viral RNA genome replication; IEA:UniProtKB-UniRule. DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW. DR Gene3D; 6.10.140.720; -; 1. DR HAMAP; MF_04065; INFV_RDRP; 1. DR InterPro; IPR007099; RNA-dir_pol_NSvirus. DR InterPro; IPR001407; RNA_pol_PB1_influenza. DR Pfam; PF00602; Flu_PB1; 1. DR PIRSF; PIRSF000827; RdRPol_OMV; 1. DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1. PE 3: Inferred from homology; KW Eukaryotic host gene expression shutoff by virus KW {ECO:0000256|ARBA:ARBA00023247, ECO:0000256|HAMAP-Rule:MF_04065}; KW Eukaryotic host transcription shutoff by virus KW {ECO:0000256|ARBA:ARBA00022731, ECO:0000256|HAMAP-Rule:MF_04065}; KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200, ECO:0000256|HAMAP- KW Rule:MF_04065}; KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995, KW ECO:0000256|HAMAP-Rule:MF_04065}; KW Host nucleus {ECO:0000256|ARBA:ARBA00022562, ECO:0000256|HAMAP- KW Rule:MF_04065}; KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581, ECO:0000256|HAMAP- KW Rule:MF_04065}; KW Inhibition of host RNA polymerase II by virus KW {ECO:0000256|ARBA:ARBA00023103, ECO:0000256|HAMAP-Rule:MF_04065}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP- KW Rule:MF_04065}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP- KW Rule:MF_04065}; KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553, ECO:0000256|HAMAP- KW Rule:MF_04065}; KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484, KW ECO:0000256|HAMAP-Rule:MF_04065}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_04065}; KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953, ECO:0000256|HAMAP- KW Rule:MF_04065}; KW Viral transcription {ECO:0000256|ARBA:ARBA00023314, ECO:0000256|HAMAP- KW Rule:MF_04065}. FT DOMAIN 286..483 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50525" FT REGION 50..82 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 249..256 FT /note="Promoter-binding site" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04065" FT MOTIF 187..195 FT /note="Nuclear localization signal" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04065" FT MOTIF 203..216 FT /note="Nuclear localization signal" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04065" SQ SEQUENCE 757 AA; 86419 MW; 1A64564C300371F9 CRC64; MDVNPTLLFL KVPVQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHKYS EKGKWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP GIFENSCLET MEIVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLTANES GRLIDFLKDV MDSMDKEEME VTTHFQRKRR VRDNMTKKMV TQRTIGKKKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG FVYFVEALAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSHDTELSF TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNVLSIA PIMFSNKMAR LGKGYMFESK SMKLRTQVPA EMLANIDLKY FNKSTREKIE KIRPLLIDGT ASLSPGMMMG MFNMLSTVLG VSILNLGQKK YTKTTYWWDG LQSSDDFALI VNAPNHEGIQ AGVDRFYRTC KLVGINMSKK KSYINRTGTF EFTSFFYRYG FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRAFELKKLW EQTRSKAGLL VSDGGPNLYN IRNLHIPEVC LKWELMDEDY QGRLCNPMNP FVSHKEIDSV NNAVVMPAHG PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF AEIMKICSTI EELRRQK //