ID A0A060VEM3_KLEPN Unreviewed; 298 AA. AC A0A060VEM3; DT 03-SEP-2014, integrated into UniProtKB/TrEMBL. DT 03-SEP-2014, sequence version 1. DT 09-DEC-2015, entry version 16. DE RecName: Full=Tyrosine recombinase XerD {ECO:0000256|HAMAP-Rule:MF_01807}; GN Name=xerD {ECO:0000256|HAMAP-Rule:MF_01807, GN ECO:0000313|EMBL:CDQ52864.1}; GN Synonyms=xerD_1 {ECO:0000313|EMBL:AIT00307.1}; GN ORFNames=AB186_18335 {ECO:0000313|EMBL:AKL30106.1}, AB187_02540 GN {ECO:0000313|EMBL:AKL44805.1}, ABY63_14085 GN {ECO:0000313|EMBL:AKK87009.1}, AMC95_13890 GN {ECO:0000313|EMBL:KOP14880.1}, AMC96_23120 GN {ECO:0000313|EMBL:KOP10085.1}, AMC97_23845 GN {ECO:0000313|EMBL:KOP07955.1}, CH1034_290013 GN {ECO:0000313|EMBL:CTQ29780.1}, KPLM21_90091 GN {ECO:0000313|EMBL:CED77671.1}, KPOL2_01260 GN {ECO:0000313|EMBL:CEN65736.1}, KPST15NDM1_01461 GN {ECO:0000313|EMBL:CEO85281.1}, KpST82_3931 GN {ECO:0000313|EMBL:CDQ52864.1}, PMK1_00810 GN {ECO:0000313|EMBL:AIT00307.1}, SM66_04130 GN {ECO:0000313|EMBL:KMH12063.1}, SM73_01987 GN {ECO:0000313|EMBL:KMH50069.1}; OS Klebsiella pneumoniae. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Klebsiella. OX NCBI_TaxID=573 {ECO:0000313|EMBL:CDQ52864.1, ECO:0000313|Proteomes:UP000035398}; RN [1] {ECO:0000313|EMBL:AIT00307.1, ECO:0000313|Proteomes:UP000029666} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=PMK1 {ECO:0000313|EMBL:AIT00307.1, RC ECO:0000313|Proteomes:UP000029666}; RX PubMed=25267672; RA Stoesser N., Giess A., Batty E.M., Sheppard A.E., Walker A.S., RA Wilson D.J., Didelot X., Bashir A., Sebra R., Kasarskis A., RA Sthapit B., Shakya M., Kelly D., Pollard A.J., Peto T.E., Crook D.W., RA Donnelly P., Thorson S., Amatya P., Joshi S.; RT "Genome Sequencing of an Extended Series of NDM-Producing Klebsiella RT pneumoniae Isolates from Neonatal Infections in a Nepali Hospital RT Characterizes the Extent of Community- versus Hospital-Associated RT Transmission in an Endemic Setting."; RL Antimicrob. Agents Chemother. 58:7347-7357(2014). RN [2] {ECO:0000313|EMBL:AKK87009.1, ECO:0000313|Proteomes:UP000035335, ECO:0000313|Proteomes:UP000035606} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CAV1344 {ECO:0000313|EMBL:AKL30106.1, RC ECO:0000313|Proteomes:UP000035335}, CAV1392 RC {ECO:0000313|EMBL:AKK87009.1, ECO:0000313|Proteomes:UP000035398}, and RC CAV1596 {ECO:0000313|EMBL:AKL44805.1, RC ECO:0000313|Proteomes:UP000035606}; RA Sheppard A.E., Stoesser N., Wilson D., Sebra R., Kasarskis A., RA Anson L., Giess A., Pankhurst L., Vaughan A., Grim C.J., Cox H., RA Yeh A., Sifri C.D., Walker S., Peto T.E., Crook D.W., Mathers A.J.; RT "Rapid spread of a carbapenem resistance gene driven by multiple RT levels of genetic mobility."; RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by CC catalyzing the cutting and rejoining of the recombining DNA CC molecules. Binds cooperatively to specific DNA consensus sequences CC that are separated from XerC binding sites by a short central CC region, forming the heterotetrameric XerC-XerD complex that CC recombines DNA substrates. The complex is essential to convert CC dimers of the bacterial chromosome into monomers to permit their CC segregation at cell division. It also contributes to the CC segregational stability of plasmids. In the complex XerD CC specifically exchanges the bottom DNA strands. {ECO:0000256|HAMAP- CC Rule:MF_01807}. CC -!- ENZYME REGULATION: FtsK may regulate the catalytic switch between CC XerC and XerD in the heterotetrameric complex during the two steps CC of the recombination process. {ECO:0000256|HAMAP-Rule:MF_01807}. CC -!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two CC molecules of XerC and two molecules of XerD, in which XerC CC interacts with XerD via its C-terminal region, XerD interacts with CC XerC via its C-terminal region and so on. {ECO:0000256|HAMAP- CC Rule:MF_01807}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01807, CC ECO:0000256|SAAS:SAAS00345948}. CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerD CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01807}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP008929; AIT00307.1; -; Genomic_DNA. DR EMBL; CP011578; AKK87009.1; -; Genomic_DNA. DR EMBL; CP011624; AKL30106.1; -; Genomic_DNA. DR EMBL; CP011647; AKL44805.1; -; Genomic_DNA. DR EMBL; LK022715; CDQ52864.1; -; Genomic_DNA. DR EMBL; CCVM01000144; CED77671.1; -; Genomic_DNA. DR EMBL; CDPE01000025; CEN65736.1; -; Genomic_DNA. DR EMBL; CDQG01000036; CEO85281.1; -; Genomic_DNA. DR EMBL; CXPD01000022; CTQ29780.1; -; Genomic_DNA. DR EMBL; LFAH01000019; KMH12063.1; -; Genomic_DNA. DR EMBL; LFAO01000015; KMH50069.1; -; Genomic_DNA. DR EMBL; LIPK01000096; KOP07955.1; -; Genomic_DNA. DR EMBL; LIPL01000102; KOP10085.1; -; Genomic_DNA. DR EMBL; LIPM01000043; KOP14880.1; -; Genomic_DNA. DR RefSeq; WP_004144729.1; NZ_LK022715.1. DR STRING; 272620.KPN_03322; -. DR EnsemblBacteria; AIT00307; AIT00307; PMK1_00810. DR EnsemblBacteria; AJB74524; AJB74524; LI86_04480. DR EnsemblBacteria; AKH00574; AKH00574; SE02_16235. DR EnsemblBacteria; AKK87009; AKK87009; ABY63_14085. DR EnsemblBacteria; AKL30106; AKL30106; AB186_18335. DR EnsemblBacteria; AKL44805; AKL44805; AB187_02540. DR EnsemblBacteria; CED77671; CED77671; KPLM21_90091. DR EnsemblBacteria; CEN65736; CEN65736; KPOL2_01260. DR EnsemblBacteria; CEO85281; CEO85281; KPST15NDM1_01461. DR EnsemblBacteria; KII58014; KII58014; SE21_11025. DR KEGG; kpb:FH42_12590; -. DR KEGG; kpne:KU54_004475; -. DR KEGG; kpnu:LI86_04480; -. DR KEGG; kpx:PMK1_00810; -. DR eggNOG; COG4974; LUCA. DR eggNOG; ENOG4105C13; Bacteria. DR KO; K04763; -. DR Proteomes; UP000029666; Chromosome. DR Proteomes; UP000035335; Chromosome. DR Proteomes; UP000035398; Chromosome. DR Proteomes; UP000035606; Chromosome. DR Proteomes; UP000036206; Unassembled WGS sequence. DR Proteomes; UP000036435; Unassembled WGS sequence. DR Proteomes; UP000039004; Unassembled WGS sequence. DR Proteomes; UP000040405; Unassembled WGS sequence. DR Proteomes; UP000046238; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-HAMAP. DR GO; GO:0006313; P:transposition, DNA-mediated; IEA:UniProtKB-HAMAP. DR Gene3D; 1.10.150.130; -; 1. DR Gene3D; 1.10.443.10; -; 1. DR HAMAP; MF_01807; Recomb_XerD; 1. DR HAMAP; MF_01808; Recomb_XerC; 1. DR InterPro; IPR011010; DNA_brk_join_enz. DR InterPro; IPR013762; Integrase-like_cat. DR InterPro; IPR002104; Integrase_catalytic. DR InterPro; IPR023109; Integrase_recombinase_N. DR InterPro; IPR004107; Integrase_SAM-like_N. DR InterPro; IPR011932; Recomb_XerD. DR InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD. DR Pfam; PF02899; Phage_int_SAM_1; 1. DR Pfam; PF00589; Phage_integrase; 1. DR SUPFAM; SSF56349; SSF56349; 1. DR TIGRFAMs; TIGR02225; recomb_XerD; 1. PE 3: Inferred from homology; KW Cell cycle {ECO:0000256|HAMAP-Rule:MF_01807, KW ECO:0000256|SAAS:SAAS00055632}; KW Cell division {ECO:0000256|HAMAP-Rule:MF_01807, KW ECO:0000256|SAAS:SAAS00055628}; KW Chromosome partition {ECO:0000256|HAMAP-Rule:MF_01807, KW ECO:0000256|SAAS:SAAS00055605}; KW Complete proteome {ECO:0000313|Proteomes:UP000029666, KW ECO:0000313|Proteomes:UP000035335, ECO:0000313|Proteomes:UP000035398, KW ECO:0000313|Proteomes:UP000035606}; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01807, KW ECO:0000256|SAAS:SAAS00019482}; KW DNA integration {ECO:0000256|HAMAP-Rule:MF_01807, KW ECO:0000256|SAAS:SAAS00019141}; KW DNA recombination {ECO:0000256|HAMAP-Rule:MF_01807, KW ECO:0000256|SAAS:SAAS00019433}; KW DNA-binding {ECO:0000256|HAMAP-Rule:MF_01807, KW ECO:0000256|SAAS:SAAS00019121}. FT DOMAIN 8 90 Phage_int_SAM_1. {ECO:0000259|Pfam: FT PF02899}. FT DOMAIN 113 284 Phage_integrase. {ECO:0000259|Pfam: FT PF00589}. FT ACT_SITE 148 148 {ECO:0000256|HAMAP-Rule:MF_01807}. FT ACT_SITE 172 172 {ECO:0000256|HAMAP-Rule:MF_01807}. FT ACT_SITE 244 244 {ECO:0000256|HAMAP-Rule:MF_01807}. FT ACT_SITE 247 247 {ECO:0000256|HAMAP-Rule:MF_01807}. FT ACT_SITE 270 270 {ECO:0000256|HAMAP-Rule:MF_01807}. FT ACT_SITE 279 279 O-(3'-phospho-DNA)-tyrosine intermediate. FT {ECO:0000256|HAMAP-Rule:MF_01807}. SQ SEQUENCE 298 AA; 33967 MW; DF893A2E8C2D2B88 CRC64; MKQDLALIEQ FLDALWLERN LAENTLSAYR RDLTMLVEWL HHRGLSLASV GSDDLQALLA ERQSGGYKAT STARLLSAVR RFFQHLYREK IRPDDPSALL ASPKLPQRLP KDLSEAQVER LLQAPLVEQP LELRDKAMLE VLYATGLRVS ELVGLTMSDI SLRQGVLRVV GKGNKERLVP LGEEAVLWVE NYLEYGRPWL LNGVASDVLF PSQRAQQMTR QTFWHRIKHY AVLAGIDSEK LSPHVLRHAF ATHLLNHGAD LRVVQMLLGH SDLSTTQIYT HVATERLRQL HQQHHPRA //