ID A0A059JDF0_TRIIM Unreviewed; 471 AA. AC A0A059JDF0; DT 09-JUL-2014, integrated into UniProtKB/TrEMBL. DT 09-JUL-2014, sequence version 1. DT 27-NOV-2024, entry version 41. DE RecName: Full=Squalene synthase {ECO:0000256|ARBA:ARBA00012373, ECO:0000256|RuleBase:RU368088}; DE Short=SQS {ECO:0000256|RuleBase:RU368088}; DE Short=SS {ECO:0000256|RuleBase:RU368088}; DE EC=2.5.1.21 {ECO:0000256|ARBA:ARBA00012373, ECO:0000256|RuleBase:RU368088}; GN ORFNames=H109_02354 {ECO:0000313|EMBL:KDB25814.1}; OS Trichophyton interdigitale (strain MR816). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Onygenales; Arthrodermataceae; Trichophyton. OX NCBI_TaxID=1215338 {ECO:0000313|EMBL:KDB25814.1, ECO:0000313|Proteomes:UP000024533}; RN [1] {ECO:0000313|EMBL:KDB25814.1, ECO:0000313|Proteomes:UP000024533} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=MR816 {ECO:0000313|EMBL:KDB25814.1, RC ECO:0000313|Proteomes:UP000024533}; RG The Broad Institute Genomics Platform; RA Cuomo C.A., White T.C., Graser Y., Martinez-Rossi N., Heitman J., RA Young S.K., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B., RA Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C., RA Imamovic A., Larimer J., Martinez D., Murphy C., Pearson M.D., RA Persinoti G., Poon T., Priest M., Roberts A.D., Saif S., Shea T.D., RA Sykes S.N., Wortman J., Nusbaum C., Birren B.; RT "The Genome Sequence of Trichophyton interdigitale MR816."; RL Submitted (FEB-2014) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) CC moieties to form squalene. {ECO:0000256|RuleBase:RU368088}. CC -!- CATALYTIC ACTIVITY: CC Reaction=2 (2E,6E)-farnesyl diphosphate + NADH + H(+) = squalene + 2 CC diphosphate + NAD(+); Xref=Rhea:RHEA:32299, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15440, ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:175763; EC=2.5.1.21; CC Evidence={ECO:0000256|RuleBase:RU368088}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2 (2E,6E)-farnesyl diphosphate + NADPH + H(+) = squalene + 2 CC diphosphate + NADP(+); Xref=Rhea:RHEA:32295, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15440, ChEBI:CHEBI:33019, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:175763; EC=2.5.1.21; CC Evidence={ECO:0000256|RuleBase:RU368088}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|ARBA:ARBA00001946, CC ECO:0000256|RuleBase:RU368088}; CC -!- PATHWAY: Terpene metabolism; lanosterol biosynthesis; lanosterol from CC farnesyl diphosphate: step 1/3. {ECO:0000256|RuleBase:RU368088}. CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family. CC {ECO:0000256|ARBA:ARBA00006251, ECO:0000256|RuleBase:RU368088}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KDB25814.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AOKY01000177; KDB25814.1; -; Genomic_DNA. DR AlphaFoldDB; A0A059JDF0; -. DR STRING; 1215338.A0A059JDF0; -. DR HOGENOM; CLU_031981_2_1_1; -. DR OMA; GEACQLM; -. DR UniPathway; UPA00767; UER00751. DR Proteomes; UP000024533; Unassembled WGS sequence. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:TreeGrafter. DR GO; GO:0004310; F:farnesyl-diphosphate farnesyltransferase activity; IEA:UniProtKB-UniRule. DR GO; GO:0051996; F:squalene synthase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006696; P:ergosterol biosynthetic process; IEA:TreeGrafter. DR GO; GO:0045338; P:farnesyl diphosphate metabolic process; IEA:InterPro. DR CDD; cd00683; Trans_IPPS_HH; 1. DR FunFam; 1.10.600.10:FF:000003; Farnesyl-diphosphate farnesyltransferase 1; 1. DR Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 1. DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf. DR InterPro; IPR002060; Squ/phyt_synthse. DR InterPro; IPR006449; Squal_synth-like. DR InterPro; IPR019845; Squalene/phytoene_synthase_CS. DR InterPro; IPR044844; Trans_IPPS_euk-type. DR InterPro; IPR033904; Trans_IPPS_HH. DR NCBIfam; TIGR01559; squal_synth; 1. DR PANTHER; PTHR11626; FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE; 1. DR PANTHER; PTHR11626:SF2; SQUALENE SYNTHASE; 1. DR Pfam; PF00494; SQS_PSY; 1. DR SFLD; SFLDS00005; Isoprenoid_Synthase_Type_I; 1. DR SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1. DR SUPFAM; SSF48576; Terpenoid synthases; 1. DR PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1. DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1. PE 3: Inferred from homology; KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022955}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022955}; KW Membrane {ECO:0000256|RuleBase:RU368088}; KW Reference proteome {ECO:0000313|Proteomes:UP000024533}; KW Steroid biosynthesis {ECO:0000256|ARBA:ARBA00022955}; KW Steroid metabolism {ECO:0000256|ARBA:ARBA00023221}; KW Sterol biosynthesis {ECO:0000256|ARBA:ARBA00023011}; KW Sterol metabolism {ECO:0000256|ARBA:ARBA00023166}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368088}; KW Transmembrane {ECO:0000256|RuleBase:RU368088}; KW Transmembrane helix {ECO:0000256|RuleBase:RU368088}. FT TRANSMEM 419..443 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU368088" SQ SEQUENCE 471 AA; 54754 MW; 1676D6229CDF877F CRC64; MKAAEIAYYL CHPQELRAII QWTTYHEPVH TRDPSKETET EKKCYEFLKL TSRSFSAVIM ELHPELLMPV CLFYLILRGL DTVEDDTSIP LETKEPILRG FKDILEEDGW TFTGNRPEEK DRELLVQFHN VITEFKKIKP AYKVIIKDIT EKMGNGMADY IRRGEEDDEI VKTIEDYDLY CYYVAGLVGE GLTRLFVEAG FARPELLERP ELFISMGRFL QKTNIIRDVR EDHDDKRRFW PREIWSRHVK EFSDLFKPEF RQQALNCNSD MILNALSHVE DCIYYLSALR EQSVFNFCCI PQTMAISTLE LCFRNGTMFE RNIKITKGTA CRLMIDSTQN VRVACDVFRR YARAIHQKNT SKDPNFLKIS MACGHVEKVI ERIFPSQSPE AAARRLTNEK SPEQLAQDEA DAEAKKDTMY IMLTIFGVLL FVTITMFFVA WLFGARFDLA IEEFKKGKIM PGPAQTHGGE L //