ID A0A059G2T5_9RHOB Unreviewed; 464 AA. AC A0A059G2T5; DT 09-JUL-2014, integrated into UniProtKB/TrEMBL. DT 09-JUL-2014, sequence version 1. DT 22-JUL-2015, entry version 11. DE RecName: Full=Fumarate hydratase class II {ECO:0000256|HAMAP-Rule:MF_00743}; DE Short=Fumarase C {ECO:0000256|HAMAP-Rule:MF_00743}; DE EC=4.2.1.2 {ECO:0000256|HAMAP-Rule:MF_00743}; GN Name=fumC {ECO:0000256|HAMAP-Rule:MF_00743, GN ECO:0000313|EMBL:KDA01034.1}; GN ORFNames=HOC_17531 {ECO:0000313|EMBL:KDA01034.1}; OS Hyphomonas oceanitis SCH89. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; OC Hyphomonadaceae; Hyphomonas. OX NCBI_TaxID=1280953 {ECO:0000313|EMBL:KDA01034.1}; RN [1] {ECO:0000313|EMBL:KDA01034.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=SCH89 {ECO:0000313|EMBL:KDA01034.1}; RX PubMed=25070064; DOI=10.1007/s10482-014-0236-y; RA Li C., Lai Q., Li G., Dong C., Wang J., Liao Y., Shao Z.; RT "Hyphomonas beringensis sp. nov. and Hyphomonas chukchiensis sp. nov., RT isolated from surface seawater of the Bering Sea and Chukchi Sea."; RL Antonie Van Leeuwenhoek 106:657-665(2014). CC -!- CATALYTIC ACTIVITY: (S)-malate = fumarate + H(2)O. CC {ECO:0000256|HAMAP-Rule:MF_00743}. CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; (S)- CC malate from fumarate: step 1/1. {ECO:0000256|HAMAP-Rule:MF_00743}. CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00743}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00743, CC ECO:0000256|SAAS:SAAS00219779}. CC -!- MISCELLANEOUS: There are 2 substrate-binding sites: the catalytic CC A site, and the non-catalytic B site that may play a role in the CC transfer of substrate or product between the active site and the CC solvent. Alternatively, the B site may bind allosteric effectors. CC {ECO:0000256|HAMAP-Rule:MF_00743}. CC -!- SIMILARITY: Belongs to the class-II fumarase/aspartase family. CC Fumarase subfamily. {ECO:0000256|HAMAP-Rule:MF_00743}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:KDA01034.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; ARYL01000038; KDA01034.1; -; Genomic_DNA. DR RefSeq; WP_035540977.1; NZ_ARYL01000038.1. DR EnsemblBacteria; KDA01034; KDA01034; HOC_17531. DR UniPathway; UPA00223; UER01007. DR GO; GO:0045239; C:tricarboxylic acid cycle enzyme complex; IEA:InterPro. DR GO; GO:0004333; F:fumarate hydratase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0006106; P:fumarate metabolic process; IEA:InterPro. DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-HAMAP. DR Gene3D; 1.10.275.10; -; 1. DR HAMAP; MF_00743; FumaraseC; 1. DR InterPro; IPR005677; Fum_hydII. DR InterPro; IPR024083; Fumarase/histidase_N. DR InterPro; IPR018951; Fumarase_C_C. DR InterPro; IPR020557; Fumarate_lyase_CS. DR InterPro; IPR000362; Fumarate_lyase_fam. DR InterPro; IPR022761; Fumarate_lyase_N. DR InterPro; IPR008948; L-Aspartase-like. DR PANTHER; PTHR11444; PTHR11444; 1. DR Pfam; PF10415; FumaraseC_C; 1. DR Pfam; PF00206; Lyase_1; 1. DR PRINTS; PR00149; FUMRATELYASE. DR SUPFAM; SSF48557; SSF48557; 1. DR TIGRFAMs; TIGR00979; fumC_II; 1. DR PROSITE; PS00163; FUMARATE_LYASES; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00743}; KW Lyase {ECO:0000256|HAMAP-Rule:MF_00743, KW ECO:0000256|SAAS:SAAS00028092}; KW Tricarboxylic acid cycle {ECO:0000256|HAMAP-Rule:MF_00743}. FT REGION 131 134 B site. {ECO:0000256|HAMAP-Rule: FT MF_00743}. FT REGION 141 143 Substrate binding. {ECO:0000256|HAMAP- FT Rule:MF_00743}. FT BINDING 102 102 Substrate. {ECO:0000256|HAMAP-Rule: FT MF_00743}. SQ SEQUENCE 464 AA; 49611 MW; 0457ACADE5A7612C CRC64; MVNTPTRTET DTMGPVEVAN DRYWGAQAER SLGNFKIGWE KQPEPIVRAL GIVKKAAAQA NMDLGKMDPE LGKVIVQVAD EVIDGKLNDH FPLVVWQTGS GTQSNMNANE VISNRAIEIL GGEMGSKKPV HPNDHVNMSQ SSNDTFPTAM HIACAEEVTH RLVPALKHLH AALDAKAKAW VDIIKIGRTH TQDATPVTLG QEFSGYAQQV ENGIARIEST LPMLMQLAQG GTAVGTGLAS PVGFAEKVAE KIAGITGLKF TSAPNKFEAL AAHDAMVFTH GAINTVAMSC FKIANDIRFL GSGPRSGLGE LALPENEPGS SIMPGKVNPT QCEALTQVCA HIHGNNAAIG FAGSQGHFEL NVFNPMMAYN FLQSTRLLAD AAISFTDNCV VGIEPRLENI ATGLKNSLML VTPLKEKYGY DRAAKIAKTA HKNGTTLREE AIKDGIPGED FDNIVDPSKM ISPG //