ID A0A031JGU5_SPHPI Unreviewed; 158 AA. AC A0A031JGU5; DT 09-JUL-2014, integrated into UniProtKB/TrEMBL. DT 09-JUL-2014, sequence version 1. DT 12-AUG-2020, entry version 29. DE RecName: Full=Transcription elongation factor GreA {ECO:0000256|HAMAP-Rule:MF_00105, ECO:0000256|RuleBase:RU000556}; DE AltName: Full=Transcript cleavage factor GreA {ECO:0000256|HAMAP-Rule:MF_00105}; GN Name=greA {ECO:0000256|HAMAP-Rule:MF_00105, GN ECO:0000313|EMBL:EZP72503.1}; GN ORFNames=BV96_01943 {ECO:0000313|EMBL:EZP72503.1}; OS Sphingomonas paucimobilis (Pseudomonas paucimobilis). OC Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; OC Sphingomonadaceae; Sphingomonas. OX NCBI_TaxID=13689 {ECO:0000313|EMBL:EZP72503.1, ECO:0000313|Proteomes:UP000023992}; RN [1] {ECO:0000313|EMBL:EZP72503.1, ECO:0000313|Proteomes:UP000023992} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EPA505 {ECO:0000313|EMBL:EZP72503.1, RC ECO:0000313|Proteomes:UP000023992}; RA Gan H.M., Gan H.Y., Chew T.H., Savka M.A.; RT "Whole genome sequence of Sphingomonas paucimobilis EPA 505."; RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription CC elongation past template-encoded arresting sites. The arresting sites CC in DNA have the property of trapping a certain fraction of elongating CC RNA polymerases that pass through, resulting in locked ternary CC complexes. Cleavage of the nascent transcript by cleavage factors such CC as GreA or GreB allows the resumption of elongation from the new CC 3'terminus. GreA releases sequences of 2 to 3 nucleotides. CC {ECO:0000256|HAMAP-Rule:MF_00105, ECO:0000256|RuleBase:RU000556}. CC -!- SIMILARITY: Belongs to the GreA/GreB family. CC {ECO:0000256|ARBA:ARBA00008213, ECO:0000256|HAMAP-Rule:MF_00105, CC ECO:0000256|RuleBase:RU000556}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:EZP72503.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JFYY01000007; EZP72503.1; -; Genomic_DNA. DR RefSeq; WP_021239387.1; NZ_JFYY01000007.1. DR EnsemblBacteria; EZP72503; EZP72503; BV96_01943. DR PATRIC; fig|13689.6.peg.1961; -. DR Proteomes; UP000023992; Unassembled WGS sequence. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro. DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:UniProtKB-UniRule. DR Gene3D; 1.10.287.180; -; 1. DR Gene3D; 3.10.50.30; -; 1. DR HAMAP; MF_00105; GreA_GreB; 1. DR InterPro; IPR036953; GreA/GreB_C_sf. DR InterPro; IPR018151; TF_GreA/GreB_CS. DR InterPro; IPR006359; Tscrpt_elong_fac_GreA. DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B. DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C. DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam. DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N. DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf. DR PANTHER; PTHR30437; PTHR30437; 1. DR Pfam; PF01272; GreA_GreB; 1. DR Pfam; PF03449; GreA_GreB_N; 1. DR PIRSF; PIRSF006092; GreA_GreB; 1. DR SUPFAM; SSF46557; SSF46557; 1. DR TIGRFAMs; TIGR01462; greA; 1. DR PROSITE; PS00830; GREAB_2; 1. PE 3: Inferred from homology; KW Coiled coil {ECO:0000256|SAM:Coils}; KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP- KW Rule:MF_00105, ECO:0000256|RuleBase:RU000556}; KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP- KW Rule:MF_00105, ECO:0000256|RuleBase:RU000556}; KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP- KW Rule:MF_00105, ECO:0000256|RuleBase:RU000556}. FT DOMAIN 8..76 FT /note="GreA_GreB_N" FT /evidence="ECO:0000259|Pfam:PF03449" FT DOMAIN 83..156 FT /note="GreA_GreB" FT /evidence="ECO:0000259|Pfam:PF01272" FT COILED 7..27 FT /evidence="ECO:0000256|SAM:Coils" FT COILED 50..70 FT /evidence="ECO:0000256|SAM:Coils" SQ SEQUENCE 158 AA; 17302 MW; 3D14BA7ECD1D1B82 CRC64; MATVEKMPML QAGYDKLTAQ LRELKAERPL IVDAIEEARA HGDLSENAEY HAAKERQGQV EATIADLEDK LSRAQIIDPA TLSGDKVIFG ATVTLLDEDD KPVKYQIVGQ AEADAKLGMI SYNSPLGRAL IGRQVGEEVE VSVPSGDKFY LVDKIEFI //