ID A0A023ZSL9_9ENTO Unreviewed; 2193 AA. AC A0A023ZSL9; DT 09-JUL-2014, integrated into UniProtKB/TrEMBL. DT 09-JUL-2014, sequence version 1. DT 16-MAR-2016, entry version 15. DE RecName: Full=Genome polyprotein {ECO:0000256|SAAS:SAAS00495321}; OS Enterovirus A71. OC Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; OC Picornavirales; Picornaviridae; Enterovirus; Enterovirus A. OX NCBI_TaxID=39054 {ECO:0000313|EMBL:AHY81310.1}; RN [1] {ECO:0000313|EMBL:AHY81310.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=M538-TW12 {ECO:0000313|EMBL:AHY81310.1}; RA Huang S.-W., Cheng H.-L., Hsieh H.-Y., Chang C.-L., Tsai H.-P., RA Kuo P.-H., Wang S.-M., Liu C.-C., Su I.-J., Wang J.-R.; RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:AHY81310.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=M538-TW12 {ECO:0000313|EMBL:AHY81310.1}; RX PubMed=24766641; DOI=10.1186/1423-0127-21-33; RA Huang S.W., Cheng H.L., Hsieh H.Y., Chang C.L., Tsai H.P., Kuo P.H., RA Wang S.M., Liu C.C., Su I.J., Wang J.R.; RT "Mutations in the non-structural protein region contribute to intra- RT genotypic evolution of enterovirus 71."; RL J. Biomed. Sci. 21:33-33(2014). CC -!- CATALYTIC ACTIVITY: NTP + H(2)O = NDP + phosphate. CC {ECO:0000256|SAAS:SAAS00495312}. CC -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate CC + RNA(n+1). {ECO:0000256|SAAS:SAAS00510455}. CC -!- CATALYTIC ACTIVITY: Selective cleavage of Gln-|-Gly bond in the CC poliovirus polyprotein. In other picornavirus reactions Glu may be CC substituted for Gln, and Ser or Thr for Gly. CC {ECO:0000256|SAAS:SAAS00495499}. CC -!- SUBCELLULAR LOCATION: Host cytoplasm CC {ECO:0000256|SAAS:SAAS00495440}. CC -!- SIMILARITY: Contains RdRp catalytic domain. CC {ECO:0000256|SAAS:SAAS00510369}. CC -!- SIMILARITY: Contains SF3 helicase domain. CC {ECO:0000256|SAAS:SAAS00503155}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KF974790; AHY81310.1; -; Genomic_RNA. DR GO; GO:0044161; C:host cell cytoplasmic vesicle; IEA:UniProtKB-KW. DR GO; GO:0044385; C:integral to membrane of host cell; IEA:UniProtKB-KW. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro. DR GO; GO:0005216; F:ion channel activity; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro. DR GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro. DR GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:UniProtKB-KW. DR GO; GO:0051259; P:protein oligomerization; IEA:UniProtKB-KW. DR GO; GO:0018144; P:RNA-protein covalent cross-linking; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW. DR Gene3D; 2.60.120.20; -; 3. DR Gene3D; 3.40.50.300; -; 1. DR Gene3D; 4.10.80.10; -; 2. DR InterPro; IPR003593; AAA+_ATPase. DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir. DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR014838; P3A. DR InterPro; IPR000081; Peptidase_C3. DR InterPro; IPR000199; Peptidase_C3A/C3B_picornavir. DR InterPro; IPR009003; Peptidase_S1_PA. DR InterPro; IPR003138; Pico_P1A. DR InterPro; IPR002527; Pico_P2B. DR InterPro; IPR001676; Picornavirus_capsid. DR InterPro; IPR001205; RNA-dir_pol_C. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR InterPro; IPR029053; Viral_coat. DR Pfam; PF08727; P3A; 1. DR Pfam; PF00548; Peptidase_C3; 1. DR Pfam; PF02226; Pico_P1A; 1. DR Pfam; PF00947; Pico_P2A; 1. DR Pfam; PF01552; Pico_P2B; 1. DR Pfam; PF00680; RdRP_1; 1. DR Pfam; PF00073; Rhv; 3. DR Pfam; PF00910; RNA_helicase; 1. DR ProDom; PD001306; Peptidase_C3; 1. DR ProDom; PD649346; Pico_P2B; 1. DR SMART; SM00382; AAA; 1. DR SUPFAM; SSF50494; SSF50494; 2. DR SUPFAM; SSF52540; SSF52540; 1. DR SUPFAM; SSF89043; SSF89043; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. DR PROSITE; PS51218; SF3_HELICASE_2; 1. PE 4: Predicted; KW ATP-binding {ECO:0000256|RuleBase:RU003299, KW ECO:0000256|SAAS:SAAS00510945}; KW Capsid protein {ECO:0000256|SAAS:SAAS00417563}; KW Helicase {ECO:0000256|SAAS:SAAS00481789}; KW Host cytoplasm {ECO:0000256|SAAS:SAAS00495503}; KW Host cytoplasmic vesicle {ECO:0000256|SAAS:SAAS00495307}; KW Host membrane {ECO:0000256|SAAS:SAAS00503033}; KW Host-virus interaction {ECO:0000256|SAAS:SAAS00495493}; KW Hydrolase {ECO:0000256|SAAS:SAAS00510997}; KW Ion channel {ECO:0000256|SAAS:SAAS00495442}; KW Ion transport {ECO:0000256|SAAS:SAAS00495456}; KW Membrane {ECO:0000256|SAAS:SAAS00503061}; KW Nucleotide-binding {ECO:0000256|RuleBase:RU003299, KW ECO:0000256|SAAS:SAAS00510884}; KW Nucleotidyltransferase {ECO:0000256|SAAS:SAAS00510590}; KW Protease {ECO:0000256|SAAS:SAAS00482667}; KW RNA-binding {ECO:0000256|SAAS:SAAS00495317}; KW RNA-directed RNA polymerase {ECO:0000256|SAAS:SAAS00510600}; KW Thiol protease {ECO:0000256|SAAS:SAAS00482329}; KW Transferase {ECO:0000256|SAAS:SAAS00510371}; KW Transport {ECO:0000256|SAAS:SAAS00495481}; KW Viral attachment to host cell {ECO:0000256|SAAS:SAAS00495304}; KW Viral ion channel {ECO:0000256|SAAS:SAAS00495447}; KW Viral RNA replication {ECO:0000256|SAAS:SAAS00511060}; KW Virion {ECO:0000256|SAAS:SAAS00417744}; KW Virus entry into host cell {ECO:0000256|SAAS:SAAS00495301}. FT DOMAIN 1216 1374 SF3 helicase. {ECO:0000259|PROSITE: FT PS51218}. FT DOMAIN 1958 2073 RdRp catalytic. {ECO:0000259|PROSITE: FT PS50507}. SQ SEQUENCE 2193 AA; 242794 MW; E7415653EFAD6125 CRC64; MGSQVSTQRS GSHENSNSAT EGSTINYTTI NYYKDSYAAT AGKQSLKQDP DKFANPVKDI FTEMAAPLKS PSAEACGYSD RVAQLTIGNS TITTQEAANI IVGYGEWPSY CSDDDATAVD KPTRPDVSVN RFYTLDTKLW EKSSKGWYWK FPDVLTETGV FGQNAQFHYL YRSGFCIHVQ CNASKFHQGA LLVAILPEYV IGTVAGGTGT EDSHPPYKQT QPGADGFELQ HPYVLDAGIP ISQLTICPHQ WINLRTNNCA TIIVPYMNTL PFDSALNHCN FGLLVVPISP LDFDQGATPV IPITITLAPM CSEFAGLRQA VTQGFPTEPK PGTNQFLTTD DGVSAPILPN FHPTPCIHIP GEVRNLLELC QVETILEVNN VPTNATSLME RLRFPVSAQA GKGELCAVFR ADPGRDGPWQ STMLGQLCGY YTQWSGSLEV TFMFTGSFMA TGKMLIAYTP PGGPLPKDRA TAMLGTHVIW DFGLQSSVTL VIPWISNTHY RAHARDGVFD YYTTGLVSIW YQTNYVVPIG APNTAYIIAL AAAQKNFTMK LCKDTSHILQ TASIQGDRVA DVIESSIGDS VSRALTQALP APTGQNTQVS SHRLDTGEVP ALQAAEIGAS SNTSDESMIE TRCVLNSHST AETTLDSFFS RAGLVGEIDL PLEGTTNPNG YANWDIDITG YAQMRRKVEL FTYMRFDAEF TFVACTPTGQ VVPQLLQYMF VPPGAPKPDS RESLAWQTAT NPSVFVKLTD PPAQVSVPFM SPASAYQWFY DGYPTFGEHK QEKDLEYGAC PNNMMGTFSV RTVGSSKSKY PLVVRIYMRM KHVRAWIPRP MRNQNYLFKA NPNYAGNSIK PTGTSRTAIT TLGKFGQQSG AIYVGNFRVV NRHLATHNDW ANLVWEDSSR DLLVSSTTAQ GCDTIARCDC QTGVYYCNSK RKHYPVSFSK PSLIYVDASE YYPARYQSHL MLAVGHSEPG DCGGILRCQH GVVGIVSTGG NGLVGFADVR DLLWLDEEAM EQGVSDYIKG LGDAFGTGFT DAVSREVEAL RNHLIGSDGA VEKILKNLIK LISALVIVIR SDYDMVTLTA TLALIGCHGS PWAWIKAKTA SILGIPIAQK QSASWLKKFN DMASAAKGLE WISNKISKFI DWLREKIVPA AREKAEFLTN LKQLPLLENQ ITNLEQSAAS QEDLEAMFGN VSYLAHFCRK FQPLYATEAK RVYVLEKRMN NYMQFKSKHR IEPVCLIIRG SPGTGKSLAT GIIARAIADK YHSSVYSLPP DPDHFDGYKQ QVVTVMDDLC QNPDGKDMSL FCQMVSTVDF IPPMASLEEK GVSFTSKFVI ASTNASNIIV PTVSDSDAIR RRFYMDCDIE VTDSYKTELG RLDAGRAAKL CSENNTANFK RCSPLVCGKA IQLRDRKSKV RYSVDTVVSE LIREYNSRSA IGNTIEALFQ GPPKFRPIRI SLEEKPAPDA ISDLLASVDS EEVRQYCREQ GWIIPETPTN VERHLNRAVL VMQSIATVVA VVSLVYVIYK LFAGFQGAYS GAPKQVLKKP VLRTATVQGP SLDFALSLLR RNIRQVQTDQ GHFTMLGVRD RLAVLPRHAQ PGKTIWVEHK LVNVLDAIEL VDEQGVNLEL TLVTLDTNEK FRDVTKFIPE TISGASDATL VINTEHMPSM FVPVGDVVQY GFLNLSGKPT HRTMMYNFPT KAGQCGGVVT SVGKIVGIHI GGNGRQGFCA GLKRSYFAST QGEIQWVKPN KETGRLNING PTHTKLEPSV FHDVFEGNKE PAVLTSKDPR LEVDFEQALF SKYVGNVLHE PDEYVTQAAL HYANQLKQLD INTSKMSMEE ACYGTENLEA IDLCTSAGYP YSALGIRKRD ILNPVTRDVS KMKLYMDKYG LDLPYSTYVK DELRSLDKIK KGKSRLIEAS SLNDSVYLRM TFGHLYEVFH ANPGTVTGSA VGCNPDVFWS KLPILLPGAL FAFDYSGYDA SLSPVWFRAL EIVLREIGYP EEAVSLIEGI NHTHHVYRNK TYCVLGGMPS GCSGTSIFNS MINNIIIRTL LIKTFKGIDL DELNMVAYGD DVLASYPFPI DCLELAKTGK EYGLTMTPAD KSPCFNEVTW ENATFLKRGF LPDYQFPFLI HPTMPMREIH ESIRWTKDAR NTQDHVRSLC LLAWHNGKDE YEKFVSTIRS VPVGKALAIP NFENLRRNWL ELF //