ID A0A023X657_9ACTN Unreviewed; 600 AA. AC A0A023X657; DT 09-JUL-2014, integrated into UniProtKB/TrEMBL. DT 09-JUL-2014, sequence version 1. DT 12-APR-2017, entry version 15. DE RecName: Full=Glutamine-dependent NAD(+) synthetase {ECO:0000256|PIRNR:PIRNR006630}; DE EC=6.3.5.1 {ECO:0000256|PIRNR:PIRNR006630}; DE AltName: Full=NAD(+) synthase [glutamine-hydrolyzing] {ECO:0000256|PIRNR:PIRNR006630}; GN ORFNames=RradSPS_2408 {ECO:0000313|EMBL:AHY47691.1}; OS Rubrobacter radiotolerans. OC Bacteria; Actinobacteria; Rubrobacteria; Rubrobacterales; OC Rubrobacteraceae; Rubrobacter. OX NCBI_TaxID=42256 {ECO:0000313|EMBL:AHY47691.1, ECO:0000313|Proteomes:UP000025229}; RN [1] {ECO:0000313|EMBL:AHY47691.1, ECO:0000313|Proteomes:UP000025229} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=RSPS-4 {ECO:0000313|EMBL:AHY47691.1, RC ECO:0000313|Proteomes:UP000025229}; RA Egas C.C., Barroso C.C., Froufe H.J.C., Pacheco J.J., RA Albuquerque L.L., da Costa M.M.S.; RT "Complete genome sequence of the Radio-Resistant Rubrobacter RT radiotolerans RSPS-4."; RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + L-glutamine + H(2)O = CC AMP + diphosphate + NAD(+) + L-glutamate. CC {ECO:0000256|PIRNR:PIRNR006630}. CC -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from CC deamido-NAD(+) (L-Gln route): step 1/1. CC {ECO:0000256|PIRNR:PIRNR006630}. CC -!- SIMILARITY: Belongs to the NAD synthetase family. CC {ECO:0000256|RuleBase:RU003811}. CC -!- SIMILARITY: In the C-terminal section; belongs to the NAD CC synthetase family. {ECO:0000256|PIRNR:PIRNR006630}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP007514; AHY47691.1; -; Genomic_DNA. DR EnsemblBacteria; AHY47691; AHY47691; RradSPS_2408. DR KEGG; rrd:RradSPS_2408; -. DR KO; K01950; -. DR UniPathway; UPA00253; UER00334. DR Proteomes; UP000025229; Chromosome. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016810; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; IEA:InterPro. DR GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro. DR GO; GO:0009435; P:NAD biosynthetic process; IEA:InterPro. DR CDD; cd00553; NAD_synthase; 1. DR Gene3D; 3.40.50.620; -; 1. DR Gene3D; 3.60.110.10; -; 1. DR InterPro; IPR003010; C-N_Hydrolase. DR InterPro; IPR014445; Gln-dep_NAD_synthase. DR InterPro; IPR022310; NAD/GMP_synthase. DR InterPro; IPR003694; NAD_synthase. DR InterPro; IPR000132; Nitrilase/CN_hydratase_CS. DR InterPro; IPR014729; Rossmann-like_a/b/a_fold. DR PANTHER; PTHR23090; PTHR23090; 1. DR Pfam; PF00795; CN_hydrolase; 1. DR Pfam; PF02540; NAD_synthase; 1. DR PIRSF; PIRSF006630; NADS_GAT; 1. DR SUPFAM; SSF56317; SSF56317; 1. DR TIGRFAMs; TIGR00552; nadE; 1. DR PROSITE; PS50263; CN_HYDROLASE; 1. DR PROSITE; PS00920; NITRIL_CHT_1; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|PIRNR:PIRNR006630, KW ECO:0000256|RuleBase:RU003811, ECO:0000256|SAAS:SAAS00702598}; KW Complete proteome {ECO:0000313|Proteomes:UP000025229}; KW Ligase {ECO:0000256|PIRNR:PIRNR006630, ECO:0000256|RuleBase:RU003811, KW ECO:0000256|SAAS:SAAS00702604}; KW NAD {ECO:0000256|PIRNR:PIRNR006630, ECO:0000256|RuleBase:RU003811, KW ECO:0000256|SAAS:SAAS00702606}; KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR006630, KW ECO:0000256|RuleBase:RU003811, ECO:0000256|SAAS:SAAS00702608}; KW Reference proteome {ECO:0000313|Proteomes:UP000025229}. FT DOMAIN 20 284 CN hydrolase. {ECO:0000259|PROSITE: FT PS50263}. SQ SEQUENCE 600 AA; 65935 MW; 7291F9822B054BF2 CRC64; MKRPPLARRG HRRRGIIWRM RVALAQVNTT VGDIWGNVEK LSEALGRAVE SGADLVAFPE LALTGYPPED LLLRPGFIGD NLKALEEFRA CVPEDVVAAV GFVDLGLDLF NACAVISGGE VLDRYHKRYL PNYGVFDENR YFREGTGSSL LDLDGTLVGV NVCEDIWYPG GPGREQALGG ASVLVNVSAS PYHRLKGATR ERMLSVRASD YGCYVVMCNL VGGQDELVFD GHSVVFDPEG ELVARAKQFE EDLLLVDIFP EEALMRRLHD SRPRKEDRTG EAPCVVSVPV SVGKVSPVES EEPRREPVLP EEGEVLAALE LGLRDYFGKN GFTKAVLGLS GGVDSSLVAA VAARALGPEN VVGVLMPSRY TSDLSNTDAY ALAEALGIET KVVPIGPAFD AYREMLADLF AGTEEDITEE NLQSRVRGNI VMGLSNKFGW IVLSTGNKSE MSVGYSTLYG DMAGGFALIR DVPKTLVYRI CRYINSSEGR EVIPESVLTK EPSAELREDQ RDSDSLPPYD VLDPILEAYI EEDKGIGQIV ALGFDEADVR RVVGLVDRAE YKRRQAPVGI KVTTRSFGRD RRMPITNRYT ERERPRLKTP //