ID A0A023H864_9INFA Unreviewed; 759 AA. AC A0A023H864; DT 09-JUL-2014, integrated into UniProtKB/TrEMBL. DT 09-JUL-2014, sequence version 1. DT 29-MAY-2024, entry version 53. DE RecName: Full=Polymerase basic protein 2 {ECO:0000256|ARBA:ARBA00014261, ECO:0000256|HAMAP-Rule:MF_04062}; DE AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000256|HAMAP-Rule:MF_04062}; GN Name=PB2 {ECO:0000256|HAMAP-Rule:MF_04062, GN ECO:0000256|RuleBase:RU361246, ECO:0000313|EMBL:AGG53297.1}; OS Influenza A virus (A/Northern shoveler/Korea/SD175/2008(H7N3)). OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; OC Alphainfluenzavirus influenzae; Influenza A virus. OX NCBI_TaxID=1293832 {ECO:0000313|EMBL:AGG53297.1}; RN [1] {ECO:0000313|EMBL:AGG53297.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=A/Northern shoveler/Korea/SD175/2008 RC {ECO:0000313|EMBL:AGG53297.1}; RA Kang H.-M., Park H.-Y., Lee K.-J., Choi J.-G., Lee E.-K., Song B.-M., RA Lee H.-S., Lee Y.-J.; RT "Ecology of H7 avian influenza viruses in wild birds in South Korea and RT their genetic comparison with novel H7N9 viruses circulating in China."; RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Plays an essential role in transcription initiation and cap- CC stealing mechanism, in which cellular capped pre-mRNAs are used to CC generate primers for viral transcription. Recognizes and binds the 7- CC methylguanosine-containing cap of the target pre-RNA which is CC subsequently cleaved after 10-13 nucleotides by the viral protein PA. CC Plays a role in the initiation of the viral genome replication and CC modulates the activity of the ribonucleoprotein (RNP) complex. CC {ECO:0000256|HAMAP-Rule:MF_04062}. CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1, CC PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). CC Interacts with nucleoprotein NP (via N-terminus). {ECO:0000256|HAMAP- CC Rule:MF_04062}. CC -!- SUBCELLULAR LOCATION: Host mitochondrion CC {ECO:0000256|ARBA:ARBA00004181}. Mitochondrion CC {ECO:0000256|ARBA:ARBA00004173}. Virion {ECO:0000256|HAMAP- CC Rule:MF_04062, ECO:0000256|RuleBase:RU361246}. Host nucleus CC {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246}. CC -!- SIMILARITY: Belongs to the influenza viruses PB2 family. CC {ECO:0000256|ARBA:ARBA00008660, ECO:0000256|HAMAP-Rule:MF_04062, CC ECO:0000256|RuleBase:RU361246}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_04062}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KC609820; AGG53297.1; -; Viral_cRNA. DR GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-UniRule. DR GO; GO:0075526; P:cap snatching; IEA:UniProtKB-UniRule. DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule. DR GO; GO:0039523; P:symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-UniRule. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR Gene3D; 3.30.30.90; Polymerase Basic Protein 2, C-terminal domain; 1. DR HAMAP; MF_04062; INV_PB2; 1. DR InterPro; IPR049110; Flu_PB2_2nd. DR InterPro; IPR049114; Flu_PB2_6th. DR InterPro; IPR049115; Flu_PB2_C. DR InterPro; IPR048298; Flu_PB2_CAP-bd. DR InterPro; IPR049111; Flu_PB2_middle. DR InterPro; IPR049106; Flu_PB2_N. DR InterPro; IPR001591; INV_PB2. DR InterPro; IPR049113; PB2_helical. DR InterPro; IPR037258; PDB2_C. DR Pfam; PF20947; Flu_PB2_1st; 1. DR Pfam; PF20948; Flu_PB2_2nd; 1. DR Pfam; PF20949; Flu_PB2_3rd; 1. DR Pfam; PF20950; Flu_PB2_4th; 1. DR Pfam; PF00604; Flu_PB2_5th; 1. DR Pfam; PF20951; Flu_PB2_6th; 1. DR Pfam; PF20952; Flu_PB2_7th; 1. DR SUPFAM; SSF160453; PB2 C-terminal domain-like; 1. PE 3: Inferred from homology; KW Cap snatching {ECO:0000256|ARBA:ARBA00022715, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW Eukaryotic host gene expression shutoff by virus KW {ECO:0000256|ARBA:ARBA00023247, ECO:0000256|HAMAP-Rule:MF_04062}; KW Eukaryotic host transcription shutoff by virus KW {ECO:0000256|ARBA:ARBA00022731, ECO:0000256|HAMAP-Rule:MF_04062}; KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995, KW ECO:0000256|HAMAP-Rule:MF_04062}; KW Host nucleus {ECO:0000256|ARBA:ARBA00022562, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW Inhibition of host RNA polymerase II by virus KW {ECO:0000256|ARBA:ARBA00023103, ECO:0000256|HAMAP-Rule:MF_04062}; KW mRNA capping {ECO:0000256|ARBA:ARBA00023042, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW mRNA processing {ECO:0000256|ARBA:ARBA00022664, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW Viral transcription {ECO:0000256|ARBA:ARBA00023314, ECO:0000256|HAMAP- KW Rule:MF_04062}; KW Virion {ECO:0000256|ARBA:ARBA00022844, ECO:0000256|HAMAP-Rule:MF_04062}. FT DOMAIN 1..36 FT /note="Influenza RNA polymerase PB2 N-terminal region" FT /evidence="ECO:0000259|Pfam:PF20947" FT DOMAIN 37..104 FT /note="Influenza RNA polymerase PB2 second" FT /evidence="ECO:0000259|Pfam:PF20948" FT DOMAIN 107..248 FT /note="Influenza RNA polymerase PB2 middle" FT /evidence="ECO:0000259|Pfam:PF20949" FT DOMAIN 250..319 FT /note="Polymerase basic protein 2 helical" FT /evidence="ECO:0000259|Pfam:PF20950" FT DOMAIN 320..530 FT /note="Influenza RNA polymerase PB2 CAP binding" FT /evidence="ECO:0000259|Pfam:PF00604" FT DOMAIN 531..680 FT /note="Influenza RNA polymerase PB2 sixth" FT /evidence="ECO:0000259|Pfam:PF20951" FT DOMAIN 683..757 FT /note="Influenza RNA polymerase PB2 C-terminal" FT /evidence="ECO:0000259|Pfam:PF20952" FT MOTIF 736..739 FT /note="Nuclear localization signal" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04062" FT SITE 627 FT /note="Avian adaptation" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04062" SQ SEQUENCE 759 AA; 85824 MW; AEC83E9079071577 CRC64; MERIKELRDL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD KRIMEMIPER NEQGQTLWSK TNDAGSDRVM VSPLAVTWWN RNGPTTSTVH YPKVYKTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKIAP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTQIG GIRMVDILRQ NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKK EEEVLTGNLQ TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGKD EQSIAEAIIV AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG IEPIDNVMGM IGILPDMTPS TEMSLRGVRV SKMGVDEYSS TERVVVSIDR FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLVNTY QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF QQMRDVLGTF DTAQIIKLLP FAAAPPEQSR MQFSSLTVNV RGSGMRILVR GNSPVFNYNK ATKRLTVLGK DAGALTEDPD EGTAGVESAV LRGFLILGKE DKRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA TKRIRMAIN //