ID A0A023H864_9INFA Unreviewed; 759 AA. AC A0A023H864; DT 09-JUL-2014, integrated into UniProtKB/TrEMBL. DT 09-JUL-2014, sequence version 1. DT 22-APR-2020, entry version 35. DE RecName: Full=Polymerase basic protein 2 {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943514}; DE AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000256|HAMAP-Rule:MF_04062}; GN Name=PB2 {ECO:0000256|HAMAP-Rule:MF_04062, GN ECO:0000256|RuleBase:RU361246, ECO:0000313|EMBL:AGG53297.1}; OS Influenza A virus (A/Northern shoveler/Korea/SD175/2008(H7N3)). OC Viruses; Riboviria; Negarnaviricota; Polyploviricotina; Insthoviricetes; OC Articulavirales; Orthomyxoviridae; Alphainfluenzavirus. OX NCBI_TaxID=1293832 {ECO:0000313|EMBL:AGG53297.1}; RN [1] {ECO:0000313|EMBL:AGG53297.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=A/Northern shoveler/Korea/SD175/2008 RC {ECO:0000313|EMBL:AGG53297.1}; RA Kang H.-M., Park H.-Y., Lee K.-J., Choi J.-G., Lee E.-K., Song B.-M., RA Lee H.-S., Lee Y.-J.; RT "Ecology of H7 avian influenza viruses in wild birds in South Korea and RT their genetic comparison with novel H7N9 viruses circulating in China."; RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Plays an essential role in transcription initiation and cap- CC stealing mechanism, in which cellular capped pre-mRNAs are used to CC generate primers for viral transcription. Recognizes and binds the 7- CC methylguanosine-containing cap of the target pre-RNA which is CC subsequently cleaved after 10-13 nucleotides by the viral protein PA. CC Plays a role in the initiation of the viral genome replication and CC modulates the activity of the ribonucleoprotein (RNP) complex. CC {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|SAAS:SAAS01109044}. CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1, CC PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). CC Interacts with nucleoprotein NP (via N-terminus). {ECO:0000256|HAMAP- CC Rule:MF_04062, ECO:0000256|SAAS:SAAS00943505}. CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000256|HAMAP-Rule:MF_04062, CC ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943502}. Virion CC {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246}. CC -!- SIMILARITY: Belongs to the influenza viruses PB2 family. CC {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246, CC ECO:0000256|SAAS:SAAS00943527}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_04062}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KC609820; AGG53297.1; -; Viral_cRNA. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-UniRule. DR GO; GO:0075526; P:cap snatching; IEA:UniProtKB-UniRule. DR GO; GO:0039523; P:suppression by virus of host RNA polymerase II activity; IEA:UniProtKB-UniRule. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR Gene3D; 3.30.30.90; -; 1. DR HAMAP; MF_04062; INV_PB2; 1. DR InterPro; IPR001591; INV_PB2. DR InterPro; IPR037258; PDB2_C. DR Pfam; PF00604; Flu_PB2; 1. DR SUPFAM; SSF160453; SSF160453; 1. PE 3: Inferred from homology; KW Cap snatching {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|SAAS:SAAS00943529}; KW Eukaryotic host gene expression shutoff by virus {ECO:0000256|HAMAP- KW Rule:MF_04062, ECO:0000256|SAAS:SAAS00943497}; KW Eukaryotic host transcription shutoff by virus {ECO:0000256|HAMAP- KW Rule:MF_04062, ECO:0000256|SAAS:SAAS00943528}; KW Host gene expression shutoff by virus {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|SAAS:SAAS00943501}; KW Host nucleus {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943496}; KW Host-virus interaction {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|SAAS:SAAS00943506}; KW Inhibition of host RNA polymerase II by virus {ECO:0000256|HAMAP- KW Rule:MF_04062, ECO:0000256|SAAS:SAAS00943508}; KW mRNA capping {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943498}; KW mRNA processing {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943500}; KW Viral transcription {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|SAAS:SAAS00943507}; KW Virion {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246, KW ECO:0000256|SAAS:SAAS00943495}. FT MOTIF 736..739 FT /note="Nuclear localization signal" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04062" FT SITE 627 FT /note="Avian adaptation" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04062" SQ SEQUENCE 759 AA; 85824 MW; AEC83E9079071577 CRC64; MERIKELRDL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD KRIMEMIPER NEQGQTLWSK TNDAGSDRVM VSPLAVTWWN RNGPTTSTVH YPKVYKTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKIAP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTQIG GIRMVDILRQ NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKK EEEVLTGNLQ TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGKD EQSIAEAIIV AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG IEPIDNVMGM IGILPDMTPS TEMSLRGVRV SKMGVDEYSS TERVVVSIDR FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLVNTY QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF QQMRDVLGTF DTAQIIKLLP FAAAPPEQSR MQFSSLTVNV RGSGMRILVR GNSPVFNYNK ATKRLTVLGK DAGALTEDPD EGTAGVESAV LRGFLILGKE DKRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA TKRIRMAIN //