{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0DYC5","uniProtkbId":"ICEN_PODCL","entryAudit":{"firstPublicDate":"2026-09-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2026-09-02","entryVersion":2,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Podila clonocystis","commonName":"Mortierella clonocystis","taxonId":979688,"lineage":["Eukaryota","Fungi","Fungi incertae sedis","Mortierellomycota","Mortierellomycotina","Mortierellomycetes","Mortierellales","Mortierellaceae","Podila"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"41811943"}],"value":"Ice-nucleating protein"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"41811943"}],"value":"INpro"}]},"flag":"Precursor"},"genes":[{"orfNames":[{"value":"BGZ82_000752"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DYC7"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41811943"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"41811943"}],"value":"Fungal ice-nucleating protein (INpro) that catalyzes ice formation at high subzero temperature through a nonmembrane-bound mechanism (PubMed:41811943). Pure water can supercool (stay liquid below 0 degrees Celsius) unless something triggers ice formation. INpros act as that trigger, allowing ice to form at relatively warmer subzero temperatures (like -2 to -5 degrees Celsius instead of -15 degrees Celsius or lower) (PubMed:41811943). Controlled ice formation outside the cell prevents sudden intracellular ice formation which is lethal (Probable). Multimerization enables high fungal ice nucleation activity and efficiency increases with the number of assembled monomers (By similarity). Exhibits extraordinary stability and potency, functioning at picomolar concentrations and under extreme conditions (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DYC7"}],"value":"Multimerizes to form homodimers, homotrimers, homotetramers and homopentamers (By similarity). Homodimer formation is mediated by stacking of tyrosine ladders between adjacent monomers (By similarity). The homotrimer, homotetramer and homopentamer formation are likely enabled by electrostatic interactions between the outward-facing positively and negatively charged residues located on opposing ends of the beta-solenoids (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41811943"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41811943"}],"value":"Contains 14 imperfect repeats of a consensus octapeptide A-G-Y-G-S-T-L-T (By similarity). Predicted to adopt beta-solenoid folds and multimerize to form extended ice-binding surfaces (PubMed:41811943)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41811943"}],"value":"The discovery of cell-free fungal INpros provides tools for innovative freezing applications and reveals biophysical constraints on ice nucleation across life (PubMed:41811943). Their membrane independence and solubility clearly distinguish them from bacterial INpros and could unlock possibilities for bioinspired freezing technologies, from precision cryopreservation to engineered weather modulation (PubMed:41811943). The fungal INpros' ability to remain highly active at low concentrations and under harsh conditions may allow them to retain function if aerosolized (PubMed:41811943)"}],"commentType":"BIOTECHNOLOGY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"41811943"}],"value":"The fungal INpros are encoded by orthologs of the bacterial InaZ gene, which was likely acquired by a fungal ancestor through horizontal gene transfer. Size characterization experiments suggest that fungal INpros are smaller than bacterial INpros and nucleate ice through a fundamentally distinct, nonmembrane-bound mechanism"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the bacterial ice nucleation protein family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":626,"modifier":"EXACT"}},"description":"Ice-nucleating protein","featureId":"PRO_0000464661"},{"type":"Repeat","location":{"start":{"value":246,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"Ice nucleation 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