{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q956K5","uniProtkbId":"Q956K5_9BASI","entryAudit":{"firstPublicDate":"2001-12-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-03-21","entryVersion":94,"sequenceVersion":2},"annotationScore":2.0,"organism":{"scientificName":"Buckleyzyma aurantiaca","taxonId":91979,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAB60968.2"}],"lineage":["Eukaryota","Fungi","Dikarya","Basidiomycota","Pucciniomycotina","Cystobasidiomycetes","Buckleyzymales","Buckleyzymaceae","Buckleyzyma"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013531"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"value":"Cytochrome b"}},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAB60968.2"}],"value":"cytb"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"value":"Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"heme b","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:60344"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"value":"Binds 2 heme groups non-covalently."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"value":"Belongs to the cytochrome b family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Transmembrane","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"Cytochrome b/b6 N-terminal region profile","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51002"}]},{"type":"Domain","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"Cytochrome b/b6 C-terminal region profile","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51003"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAB60968.2"}]},{"type":"Non-terminal residue","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAB60968.2"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAB60968.2"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022617"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"id":"KW-0349","category":"Ligand","name":"Heme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"11411687","citationType":"journal article","authors":["Biswas S.K.","Yokoyama K.","Nishimura K.","Miyaji M."],"citationCrossReferences":[{"database":"PubMed","id":"11411687"}],"title":"Molecular phylogenetics of the genus Rhodotorula and related basidiomycetous yeasts inferred from the mitochondrial cytochrome b gene.","publicationDate":"2001","journal":"Int. J. Syst. Evol. Microbiol.","firstPage":"1191","lastPage":"1199","volume":"51"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAB60968.2"}],"value":"IFM 40059","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAB60968.2"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB040616","properties":[{"key":"ProteinId","value":"BAB60968.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q956K5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008121","properties":[{"key":"GoTerm","value":"F:quinol-cytochrome-c reductase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006122","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, ubiquinol to cytochrome c"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"Gene3D","id":"1.20.810.10","properties":[{"key":"EntryName","value":"Cytochrome Bc1 Complex, Chain C"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005798","properties":[{"key":"EntryName","value":"Cyt_b/b6_C"}]},{"database":"InterPro","id":"IPR036150","properties":[{"key":"EntryName","value":"Cyt_b/b6_C_sf"}]},{"database":"InterPro","id":"IPR005797","properties":[{"key":"EntryName","value":"Cyt_b/b6_N"}]},{"database":"InterPro","id":"IPR027387","properties":[{"key":"EntryName","value":"Cytb/b6-like_sf"}]},{"database":"InterPro","id":"IPR016174","properties":[{"key":"EntryName","value":"Di-haem_cyt_TM"}]},{"database":"PANTHER","id":"PTHR19271","properties":[{"key":"EntryName","value":"CYTOCHROME B"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR19271:SF16","properties":[{"key":"EntryName","value":"CYTOCHROME B"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00032","properties":[{"key":"EntryName","value":"Cytochrom_B_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13631","properties":[{"key":"EntryName","value":"Cytochrom_B_N_2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81648","properties":[{"key":"EntryName","value":"a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81342","properties":[{"key":"EntryName","value":"Transmembrane di-heme cytochromes"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51003","properties":[{"key":"EntryName","value":"CYTB_CTER"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51002","properties":[{"key":"EntryName","value":"CYTB_NTER"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"NVLSAIPWIGQDFVQFVWGGFSVNNATLNRFYSLHFLLPFVLAALAAMHMLTIHEHGSGNPLGLSANTDRIPFHPYFTFKDLITIFGFFLALSAIVFYAPNLLGHSDNYIPANPMQTPPSIVPEWYLLPYYA","length":132,"molWeight":14833,"crc64":"3CDD927BBBC34AFC","md5":"5C907A7349000E0F6A8D51B94F99244A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Transmembrane":2,"Domain":2,"Non-terminal residue":2},"uniParcId":"UPI000247F4CE"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q5R2I0","uniProtkbId":"Q5R2I0_9BASI","entryAudit":{"firstPublicDate":"2005-01-04","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-01-04","entryVersion":56,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Buckleyzyma aurantiaca","taxonId":91979,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAD74127.1"}],"lineage":["Eukaryota","Fungi","Dikarya","Basidiomycota","Pucciniomycotina","Cystobasidiomycetes","Buckleyzymales","Buckleyzymaceae","Buckleyzyma"]},"proteinExistence":"4: Predicted","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAD74127.1"}],"value":"Elongation factor-1 alpha"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAD74127.1"}],"value":"tef-1"}}],"comments":[{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"}],"value":"Cytoplasm","id":"SL-0086"}}]}],"features":[{"type":"Domain","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"Translation elongation factor EFTu-like","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF03144"}]},{"type":"Domain","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"GTP-eEF1A C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF22594"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAD74127.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":230,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAD74127.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022768"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAD74127.1"}],"id":"KW-0251","category":"Molecular function","name":"Elongation factor"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022917"}],"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"16598157","citationType":"journal article","authors":["Nagahama T.","Hamamoto M.","Nakase T.","Shimamura S.","Horikoshi K."],"citationCrossReferences":[{"database":"PubMed","id":"16598157"},{"database":"DOI","id":"10.2323/jgam.52.37"}],"title":"Phylogenetic relationship within the Erythrobasidium clade: molecular phylogenies, secondary structure, and intron positions inferred from partial sequences of ribosomal RNA and elongation factor-1alpha genes.","publicationDate":"2006","journal":"J. Gen. Appl. Microbiol.","firstPage":"37","lastPage":"45","volume":"52"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAD74127.1"}],"value":"JCM 3771","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAD74127.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB127081","properties":[{"key":"ProteinId","value":"BAD74127.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q5R2I0","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd03693","properties":[{"key":"EntryName","value":"EF1_alpha_II"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.30.10:FF:000003","properties":[{"key":"EntryName","value":"Elongation factor 1-alpha"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR004161","properties":[{"key":"EntryName","value":"EFTu-like_2"}]},{"database":"InterPro","id":"IPR054696","properties":[{"key":"EntryName","value":"GTP-eEF1A_C"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR050100","properties":[{"key":"EntryName","value":"TRAFAC_GTPase_members"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"PANTHER","id":"PTHR23115","properties":[{"key":"EntryName","value":"TRANSLATION FACTOR"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22594","properties":[{"key":"EntryName","value":"GTP-eEF1A_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03144","properties":[{"key":"EntryName","value":"GTP_EFTU_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WSESRFEEIIKETSGFIKKVGFNPKQVAFVPISGWHGDNMLEESPNMTWYKGWKKETKSGEVKGKTLLEAIDAVEPPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGTIKAGMVVTFAPSAVTTEVKSVEMHHEQLEAGLPGDNVGFNVKNVSVKDIRRGNVAGDSKNDPPKGCASFNAQVIVLNHPGQIGNGYSPVLDCHTAHIACKFDELLEKIDRRTGKSIEDKP","length":230,"molWeight":25133,"crc64":"775351C1A9851324","md5":"940D0C30A8A6AD8BABBC53644B6BE11F"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1},"countByFeatureType":{"Domain":2,"Non-terminal residue":2},"uniParcId":"UPI000048F419"}}]}