{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q06319","uniProtkbId":"ACDS_MEGEL","entryAudit":{"firstPublicDate":"1996-10-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"1996-10-01","entryVersion":125,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Acyl-CoA dehydrogenase, short-chain specific"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8399220"}],"value":"1.3.8.1"}]},"alternativeNames":[{"fullName":{"value":"Butyryl-CoA dehydrogenase"},"shortNames":[{"value":"BCAD"}]},{"fullName":{"value":"SCAD"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8399220"}],"value":"Has an optimum specificity for 4-carbon length fatty acyl-CoAs"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24004"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57332"},{"database":"ChEBI","id":"CHEBI:57371"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"}],"ecNumber":"1.3.8.1","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8399220"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a short-chain 2,3-saturated fatty acyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = a short-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:47196"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"},{"database":"ChEBI","id":"CHEBI:87487"},{"database":"ChEBI","id":"CHEBI:87488"}],"ecNumber":"1.3.8.1","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P52042"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"FAD","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:57692"}}]},{"texts":[{"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the acyl-CoA dehydrogenase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":383,"modifier":"EXACT"}},"description":"Acyl-CoA dehydrogenase, short-chain specific","featureId":"PRO_0000201190"},{"type":"Active site","location":{"start":{"value":367,"modifier":"EXACT"},"end":{"value":367,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8399220"}]},{"type":"Mutagenesis","location":{"start":{"value":367,"modifier":"EXACT"},"end":{"value":367,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8399220"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Helix","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Turn","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":93,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Turn","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":168,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":181,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":197,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":225,"modifier":"EXACT"},"end":{"value":227,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Beta strand","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":279,"modifier":"EXACT"},"end":{"value":281,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":283,"modifier":"EXACT"},"end":{"value":312,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":317,"modifier":"EXACT"},"end":{"value":342,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":343,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":353,"modifier":"EXACT"},"end":{"value":360,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":361,"modifier":"EXACT"},"end":{"value":364,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Turn","location":{"start":{"value":365,"modifier":"EXACT"},"end":{"value":367,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]},{"type":"Helix","location":{"start":{"value":370,"modifier":"EXACT"},"end":{"value":381,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BUC"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0274","category":"Ligand","name":"FAD"},{"id":"KW-0276","category":"Biological process","name":"Fatty acid metabolism"},{"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"8399220","citationType":"journal article","authors":["Becker D.F.","Fuchs J.A.","Banfield D.K.","Funk W.D.","Macgillivray R.T.A.","Stankovich M.T."],"citationCrossReferences":[{"database":"PubMed","id":"8399220"},{"database":"DOI","id":"10.1021/bi00091a026"}],"title":"Characterization of wild-type and an active-site mutant in Escherichia coli of short-chain acyl-CoA dehydrogenase from Megasphaera elsdenii.","publicationDate":"1993","journal":"Biochemistry","firstPage":"10736","lastPage":"10742","volume":"32"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","PROTEIN SEQUENCE OF 1-50","FUNCTION","CATALYTIC ACTIVITY","ACTIVE SITE","MUTAGENESIS OF GLU-367"]},{"referenceNumber":2,"citation":{"id":"7857927","citationType":"journal article","authors":["Djordjevic S.","Pace C.P.","Stankovich M.T.","Kim J.-J.P."],"citationCrossReferences":[{"database":"PubMed","id":"7857927"},{"database":"DOI","id":"10.1021/bi00007a009"}],"title":"Three-dimensional structure of butyryl-CoA dehydrogenase from Megasphaera elsdenii.","publicationDate":"1995","journal":"Biochemistry","firstPage":"2163","lastPage":"2171","volume":"34"},"referencePositions":["X-RAY CRYSTALLOGRAPHY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L04528","properties":[{"key":"ProteinId","value":"AAA03594.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Unassigned_DNA"}]},{"database":"RefSeq","id":"WP_014017064.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_AP031433.1"}]},{"database":"PDB","id":"1BUC","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"A/B=1-383"}]},{"database":"AlphaFoldDB","id":"Q06319","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q06319","properties":[{"key":"Description","value":"-"}]},{"database":"DrugBank","id":"DB03059","properties":[{"key":"GenericName","value":"Acetoacetyl-CoA"}]},{"database":"DrugBank","id":"DB03147","properties":[{"key":"GenericName","value":"Flavin adenine dinucleotide"}]},{"database":"GeneID","id":"97492323","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9802447at2","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-11937","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"Q06319","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0050660","properties":[{"key":"GoTerm","value":"F:flavin adenine dinucleotide binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016937","properties":[{"key":"GoTerm","value":"F:short-chain fatty acyl-CoA dehydrogenase activity"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8399220"}]},{"database":"CDD","id":"cd01158","properties":[{"key":"EntryName","value":"SCAD_SBCAD"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.540.10:FF:000002","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase FadE19"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.140.10:FF:000004","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase FadE25"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.110.10:FF:000001","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase, mitochondrial"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.540.10","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase/oxidase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.110.10","properties":[{"key":"EntryName","value":"Butyryl-CoA Dehydrogenase, subunit A, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.140.10","properties":[{"key":"EntryName","value":"Butyryl-CoA Dehydrogenase, subunit A, domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006089","properties":[{"key":"EntryName","value":"Acyl-CoA_DH_CS"}]},{"database":"InterPro","id":"IPR046373","properties":[{"key":"EntryName","value":"Acyl-CoA_Oxase/DH_mid-dom_sf"}]},{"database":"InterPro","id":"IPR036250","properties":[{"key":"EntryName","value":"AcylCo_DH-like_C"}]},{"database":"InterPro","id":"IPR009075","properties":[{"key":"EntryName","value":"AcylCo_DH/oxidase_C"}]},{"database":"InterPro","id":"IPR006091","properties":[{"key":"EntryName","value":"AcylCoA_DH/ox_M"}]},{"database":"InterPro","id":"IPR013786","properties":[{"key":"EntryName","value":"AcylCoA_DH/ox_N"}]},{"database":"InterPro","id":"IPR037069","properties":[{"key":"EntryName","value":"AcylCoA_DH/ox_N_sf"}]},{"database":"InterPro","id":"IPR009100","properties":[{"key":"EntryName","value":"AcylCoA_DH/oxidase_NM_dom_sf"}]},{"database":"PANTHER","id":"PTHR43884","properties":[{"key":"EntryName","value":"ACYL-COA DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43884:SF12","properties":[{"key":"EntryName","value":"ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00441","properties":[{"key":"EntryName","value":"Acyl-CoA_dh_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02770","properties":[{"key":"EntryName","value":"Acyl-CoA_dh_M"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02771","properties":[{"key":"EntryName","value":"Acyl-CoA_dh_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF016578","properties":[{"key":"EntryName","value":"HsaA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF47203","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56645","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase NM domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00072","properties":[{"key":"EntryName","value":"ACYL_COA_DH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00073","properties":[{"key":"EntryName","value":"ACYL_COA_DH_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDFNLTDIQQDFLKLAHDFGEKKLAPTVTERDHKGIYDKELIDELLSLGITGAYFEEKYGGSGDDGGDVLSYILAVEELAKYDAGVAITLSATVSLCANPIWQFGTEAQKEKFLVPLVEGTKLGAFGLTEPNAGTDASGQQTIATKNDDGTYTLNGSKIFITNGGAADIYIVFAMTDKSKGNHGITAFILEDGTPGFTYGKKEDKMGIHTSQTMELVFQDVKVPAENMLGEEGKGFKIAMMTLDGGRIGVAAQALGIAEAALADAVEYSKQRVQFGKPLCKFQSISFKLADMKMQIEAARNLVYKAACKKQEGKPFTVDAAIAKRVASDVAMRVTTEAVQIFGGYGYSEEYPVARHMRDAKITQIYEGTNEVQLMVTGGALLR","length":383,"molWeight":41408,"crc64":"3D68AAE34D9BBAB8","md5":"73C933C37924C395FFBA71834B242CB6"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1,"Mutagenesis":1,"Helix":24,"Turn":3,"Beta strand":10},"uniParcId":"UPI0000110A54"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P00271","uniProtkbId":"RUBR_MEGEL","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"1986-07-21","entryVersion":88,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Rubredoxin"},"shortNames":[{"value":"Rd"}]}},"comments":[{"texts":[{"value":"Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Fe(3+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29034"}}],"note":{"texts":[{"value":"Binds 1 Fe(3+) ion per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the rubredoxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"Rubredoxin","featureId":"PRO_0000135041"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"Rubredoxin-like","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00241"}]},{"type":"Binding site","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":6,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875"}},{"type":"Binding site","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":9,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875"}},{"type":"Binding site","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875"}},{"type":"Binding site","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875"}}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"5640967","citationType":"journal article","authors":["Bachmayer H.","Yasunobu K.T.","Peel J.L.","Mayhew S.G."],"citationCrossReferences":[{"database":"PubMed","id":"5640967"},{"database":"DOI","id":"10.1016/s0021-9258(18)93617-3"}],"title":"Non-heme iron proteins. V. The amino acid sequence of rubredoxin from Peptostreptococcus elsdenii.","publicationDate":"1968","journal":"J. Biol. Chem.","firstPage":"1022","lastPage":"1030","volume":"243"},"referencePositions":["PROTEIN SEQUENCE"]}],"uniProtKBCrossReferences":[{"database":"PIR","id":"A00277","properties":[{"key":"EntryName","value":"RUME"}]},{"database":"AlphaFoldDB","id":"P00271","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P00271","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0043448","properties":[{"key":"GoTerm","value":"P:alkane catabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd00730","properties":[{"key":"EntryName","value":"rubredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.20.28.10:FF:000001","properties":[{"key":"EntryName","value":"Rubredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.20.28.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR024922","properties":[{"key":"EntryName","value":"Rubredoxin"}]},{"database":"InterPro","id":"IPR024934","properties":[{"key":"EntryName","value":"Rubredoxin-like_dom"}]},{"database":"InterPro","id":"IPR024935","properties":[{"key":"EntryName","value":"Rubredoxin_dom"}]},{"database":"InterPro","id":"IPR050526","properties":[{"key":"EntryName","value":"Rubredoxin_ET"}]},{"database":"InterPro","id":"IPR018527","properties":[{"key":"EntryName","value":"Rubredoxin_Fe_BS"}]},{"database":"PANTHER","id":"PTHR47627","properties":[{"key":"EntryName","value":"RUBREDOXIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47627:SF1","properties":[{"key":"EntryName","value":"RUBREDOXIN-1-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00301","properties":[{"key":"EntryName","value":"Rubredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000071","properties":[{"key":"EntryName","value":"Rubredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00163","properties":[{"key":"EntryName","value":"RUBREDOXIN"}]},{"database":"SUPFAM","id":"SSF57802","properties":[{"key":"EntryName","value":"Rubredoxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00202","properties":[{"key":"EntryName","value":"RUBREDOXIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50903","properties":[{"key":"EntryName","value":"RUBREDOXIN_LIKE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDKYECSICGYIYDEAEGDDGNVAAGTKFADLPADWVCPTCGADKDAFVKMD","length":52,"molWeight":5616,"crc64":"A13AB077992FDFD4","md5":"19D7D5A97B361BC34340C3DB8D517DC6"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Binding site":4},"uniParcId":"UPI0000135311"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O85691","uniProtkbId":"ETFB_MEGEL","entryAudit":{"firstPublicDate":"2000-05-30","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1998-11-01","entryVersion":76,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Electron transfer flavoprotein subunit beta"},"shortNames":[{"value":"Beta-ETF"}]},"alternativeNames":[{"fullName":{"value":"Electron transfer flavoprotein small subunit"},"shortNames":[{"value":"ETFSS"}]}]},"genes":[{"geneName":{"value":"etfB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53570"}],"value":"Heterodimeric electron transfer flavoprotein that accepts electrons from dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). EtfB binds an AMP molecule that probably has a purely structural role"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53570"}],"value":"Heterodimer composed of an alpha and beta subunit"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ETF alpha-subunit/FixB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"Electron transfer flavoprotein subunit beta","featureId":"PRO_0000167879"},{"type":"Binding site","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:456215"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53570"}],"ligand":{"name":"AMP","id":"ChEBI:CHEBI:456215"}},{"type":"Binding site","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:456215"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53570"}],"ligand":{"name":"AMP","id":"ChEBI:CHEBI:456215"}},{"type":"Binding site","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:456215"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53570"}],"ligand":{"name":"AMP","id":"ChEBI:CHEBI:456215"}},{"type":"Binding site","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:456215"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53570"}],"ligand":{"name":"AMP","id":"ChEBI:CHEBI:456215"}}],"keywords":[{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-0274","category":"Ligand","name":"FAD"},{"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"9694853","citationType":"journal article","authors":["O'Neill H.","Mayhew S.G.","Butler G."],"citationCrossReferences":[{"database":"PubMed","id":"9694853"},{"database":"DOI","id":"10.1074/jbc.273.33.21015"}],"title":"Cloning and analysis of the genes for a novel electron-transferring flavoprotein from Megasphaera elsdenii. Expression and characterization of the recombinant protein.","publicationDate":"1998","journal":"J. Biol. Chem.","firstPage":"21015","lastPage":"21024","volume":"273"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 25940 / DSM 20460 / JCM 1772 / NCIB 8927","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF072475","properties":[{"key":"ProteinId","value":"AAC31169.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_014017063.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CATZAM010000006.1"}]},{"database":"AlphaFoldDB","id":"O85691","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O85691","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"97492324","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-21346","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd01714","properties":[{"key":"EntryName","value":"ETF_beta"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.620","properties":[{"key":"EntryName","value":"HUPs"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR014730","properties":[{"key":"EntryName","value":"ETF_a/b_N"}]},{"database":"InterPro","id":"IPR012255","properties":[{"key":"EntryName","value":"ETF_b"}]},{"database":"InterPro","id":"IPR033948","properties":[{"key":"EntryName","value":"ETF_beta_N"}]},{"database":"InterPro","id":"IPR014729","properties":[{"key":"EntryName","value":"Rossmann-like_a/b/a_fold"}]},{"database":"PANTHER","id":"PTHR21294","properties":[{"key":"EntryName","value":"ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21294:SF17","properties":[{"key":"EntryName","value":"PROTEIN FIXA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01012","properties":[{"key":"EntryName","value":"ETF"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000090","properties":[{"key":"EntryName","value":"Beta-ETF"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00893","properties":[{"key":"EntryName","value":"ETF"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52402","properties":[{"key":"EntryName","value":"Adenine nucleotide alpha hydrolases-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEILVCVKQVPDTAEVKIDPVKHTVIRAGVPNIFNPFDQNALEAALALKDADKDVKITLLSMGPDQAKDVLREGLAMGADDAYLLSDRKLGGSDTLATGYALAQAIKKLAADKGIEQFDIILCGKQAIDGDTAQVGPQIACELGIPQITYARDIKVEGDKVTVQQENEEGYIVTEAQFPVLITAVKDLNEPRFPTIRGTMKAKRREIPNLDAAAVAADDAQIGLSGSPTKVRKIFTPPQRSGGLVLKVEDDNEQAIVDQVMEKLVAQKII","length":270,"molWeight":29099,"crc64":"9AC0A46E8FA93397","md5":"F0BB7E20E5C082AB5CB760354E641D45"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":4},"uniParcId":"UPI000012A24E"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P00201","uniProtkbId":"FER_MEGEL","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1986-07-21","entryVersion":92,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Ferredoxin"}}},"comments":[{"texts":[{"value":"Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"[4Fe-4S] cluster","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49883"}}],"note":{"texts":[{"value":"Binds 2 [4Fe-4S] clusters."}]}}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"Ferredoxin","featureId":"PRO_0000159117"},{"type":"Domain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"4Fe-4S ferredoxin-type 1","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00711"}]},{"type":"Domain","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"4Fe-4S ferredoxin-type 2","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00711"}]},{"type":"Binding site","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":8,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"1"}},{"type":"Binding site","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"1"}},{"type":"Binding site","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":14,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"1"}},{"type":"Binding site","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"2"}},{"type":"Binding site","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"2"}},{"type":"Binding site","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"2"}},{"type":"Binding site","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"2"}},{"type":"Binding site","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"1"}}],"keywords":[{"id":"KW-0004","category":"Ligand","name":"4Fe-4S"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-ES4NDHKI5CICE","citationType":"journal article","authors":["Yasunobu K.T.","Tanaka M."],"title":"The evolution of iron-sulfur protein containing organisms.","publicationDate":"1973","journal":"Syst. Zool.","firstPage":"570","lastPage":"589","volume":"22"},"referencePositions":["PROTEIN SEQUENCE"]}],"uniProtKBCrossReferences":[{"database":"PIR","id":"A00203","properties":[{"key":"EntryName","value":"FEME"}]},{"database":"AlphaFoldDB","id":"P00201","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P00201","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.30.70.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR017896","properties":[{"key":"EntryName","value":"4Fe4S_Fe-S-bd"}]},{"database":"InterPro","id":"IPR017900","properties":[{"key":"EntryName","value":"4Fe4S_Fe_S_CS"}]},{"database":"InterPro","id":"IPR000813","properties":[{"key":"EntryName","value":"7Fe_ferredoxin"}]},{"database":"InterPro","id":"IPR050157","properties":[{"key":"EntryName","value":"PSI_iron-sulfur_center"}]},{"database":"PANTHER","id":"PTHR24960:SF79","properties":[{"key":"EntryName","value":"PHOTOSYSTEM I IRON-SULFUR CENTER"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR24960","properties":[{"key":"EntryName","value":"PHOTOSYSTEM I IRON-SULFUR CENTER-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13187","properties":[{"key":"EntryName","value":"Fer4_9"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00354","properties":[{"key":"EntryName","value":"7FE8SFRDOXIN"}]},{"database":"SUPFAM","id":"SSF54862","properties":[{"key":"EntryName","value":"4Fe-4S ferredoxins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00198","properties":[{"key":"EntryName","value":"4FE4S_FER_1"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51379","properties":[{"key":"EntryName","value":"4FE4S_FER_2"},{"key":"MatchStatus","value":"2"}]}],"sequence":{"value":"MHVISDECVKCGACASTCPTGAIEEGETKYVVTDSCIDCGACEAVCPTGAISAE","length":54,"molWeight":5430,"crc64":"90C6BC38EAE3A577","md5":"436BD1397FDB39BDD133B4E37FC061DB"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1},"countByFeatureType":{"Chain":1,"Domain":2,"Binding site":8},"uniParcId":"UPI000012A6C4"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O85692","uniProtkbId":"ETFA_MEGEL","entryAudit":{"firstPublicDate":"2000-05-30","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1998-11-01","entryVersion":81,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Electron transfer flavoprotein subunit alpha"},"shortNames":[{"value":"Alpha-ETF"}]},"alternativeNames":[{"fullName":{"value":"Electron transfer flavoprotein large subunit"},"shortNames":[{"value":"ETFLS"}]}]},"genes":[{"geneName":{"value":"etfA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"value":"Heterodimeric electron transfer flavoprotein that accepts electrons from dehydrogenases. Transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"value":"Heterodimer composed of an alpha and beta subunit"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ETF alpha-subunit/FixB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":338,"modifier":"EXACT"}},"description":"Electron transfer flavoprotein subunit alpha","featureId":"PRO_0000167850"},{"type":"Binding site","location":{"start":{"value":222,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":249,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":250,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":267,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":268,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":282,"modifier":"EXACT"},"end":{"value":282,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":286,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":319,"modifier":"EXACT"},"end":{"value":319,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":320,"modifier":"EXACT"},"end":{"value":320,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P53571"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}}],"keywords":[{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-0274","category":"Ligand","name":"FAD"},{"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"9694853","citationType":"journal article","authors":["O'Neill H.","Mayhew S.G.","Butler G."],"citationCrossReferences":[{"database":"PubMed","id":"9694853"},{"database":"DOI","id":"10.1074/jbc.273.33.21015"}],"title":"Cloning and analysis of the genes for a novel electron-transferring flavoprotein from Megasphaera elsdenii. Expression and characterization of the recombinant protein.","publicationDate":"1998","journal":"J. Biol. Chem.","firstPage":"21015","lastPage":"21024","volume":"273"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 25940 / DSM 20460 / JCM 1772 / NCIB 8927","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF072475","properties":[{"key":"ProteinId","value":"AAC31170.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"O85692","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O85692","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-21345","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0050660","properties":[{"key":"GoTerm","value":"F:flavin adenine dinucleotide binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0033539","properties":[{"key":"GoTerm","value":"P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd01715","properties":[{"key":"EntryName","value":"ETF_alpha"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.620","properties":[{"key":"EntryName","value":"HUPs"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1220","properties":[{"key":"EntryName","value":"TPP-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR029035","properties":[{"key":"EntryName","value":"DHS-like_NAD/FAD-binding_dom"}]},{"database":"InterPro","id":"IPR014730","properties":[{"key":"EntryName","value":"ETF_a/b_N"}]},{"database":"InterPro","id":"IPR001308","properties":[{"key":"EntryName","value":"ETF_a/FixB"}]},{"database":"InterPro","id":"IPR033947","properties":[{"key":"EntryName","value":"ETF_alpha_N"}]},{"database":"InterPro","id":"IPR014731","properties":[{"key":"EntryName","value":"ETF_asu_C"}]},{"database":"InterPro","id":"IPR018206","properties":[{"key":"EntryName","value":"ETF_asu_C_CS"}]},{"database":"InterPro","id":"IPR014729","properties":[{"key":"EntryName","value":"Rossmann-like_a/b/a_fold"}]},{"database":"PANTHER","id":"PTHR43153","properties":[{"key":"EntryName","value":"ELECTRON TRANSFER FLAVOPROTEIN ALPHA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43153:SF1","properties":[{"key":"EntryName","value":"ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01012","properties":[{"key":"EntryName","value":"ETF"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00766","properties":[{"key":"EntryName","value":"ETF_alpha"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000089","properties":[{"key":"EntryName","value":"Electra_flavoP_a"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00893","properties":[{"key":"EntryName","value":"ETF"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52402","properties":[{"key":"EntryName","value":"Adenine nucleotide alpha hydrolases-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52467","properties":[{"key":"EntryName","value":"DHS-like NAD/FAD-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00696","properties":[{"key":"EntryName","value":"ETF_ALPHA"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDLAEYKGIYVIAEQFEGKLRDVSFELLGQARILADTIGDEVGAILIGKDVKPLAQELIAHGAHKVYVYDDPQLEHYNTTAYAKVICDFFHEEKPNVFLVGATNIGRDLGPRVANSLKTGLTADCTQLGVDDDKKTIVWTRPALGGNIMAEIICPDNRPQMGTVRPHVFKKPEADPSATGEVIEKKANLSDADFMTKFVELIKLGGEGVKIEDADVIVAGGRGMNSEEPFKTGILKECADVLGGAVGASRAAVDAGWIDALHQVGQTGKTVGPKIYIACAISGAIQPLAGMTGSDCIIAINKDEDAPIFKVCDYGIVGDVFKVLPLLTEAIKKQKGIA","length":338,"molWeight":36125,"crc64":"63FBD4CCF111AE77","md5":"53F48A380E5C1BDEA3632D9F67DD4359"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":12},"uniParcId":"UPI000012A247"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P00321","uniProtkbId":"FLAV_MEGEL","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"1986-07-21","entryVersion":105,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Flavodoxin"}}},"comments":[{"texts":[{"value":"Low-potential electron donor to a number of redox enzymes"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"FMN","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:58210"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the flavodoxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"Flavodoxin","featureId":"PRO_0000171639"},{"type":"Domain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"Flavodoxin-like","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00088"}]},{"type":"Beta strand","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":6,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Beta strand","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Helix","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Beta strand","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Helix","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Beta strand","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Turn","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Helix","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Helix","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Beta strand","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Helix","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Beta strand","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]},{"type":"Helix","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FZ5"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"id":"KW-0288","category":"Ligand","name":"FMN"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"4711610","citationType":"journal article","authors":["Tanaka M.","Haniu M.","Yasunobu K.T.","Mayhew S.G.","Massey V."],"citationCrossReferences":[{"database":"PubMed","id":"4711610"},{"database":"DOI","id":"10.1016/s0021-9258(19)43779-4"}],"title":"The primary structure of Peptostreptococcus elsdenii flavodoxin.","publicationDate":"1973","journal":"J. Biol. Chem.","firstPage":"4354","lastPage":"4366","volume":"248"},"referencePositions":["PROTEIN SEQUENCE"],"referenceComments":[{"value":"ATCC 25940 / DSM 20460 / JCM 1772 / NCIB 8927","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"4843143","citationType":"journal article","authors":["Tanaka M.","Haniu M.","Yasunobu K.T.","Mayhew S.G.","Massey V."],"citationCrossReferences":[{"database":"PubMed","id":"4843143"},{"database":"DOI","id":"10.1016/s0021-9258(19)42431-9"}],"title":"Correction of the amino acid sequence of Peptostreptococcus elsdenii flavodoxin.","publicationDate":"1974","journal":"J. Biol. Chem.","firstPage":"4397","lastPage":"4397","volume":"249"},"referencePositions":["SEQUENCE REVISION TO 78-82"]},{"referenceNumber":3,"citation":{"id":"5126921","citationType":"journal article","authors":["Tanaka M.","Haniu M.","Matsueda G.","Yasunobu K.T.","Mayhew S.","Massey V."],"citationCrossReferences":[{"database":"PubMed","id":"5126921"},{"database":"DOI","id":"10.1021/bi00792a009"}],"title":"Amino- and carboxyl-terminal amino acid sequences of the Peptostreptococcus eisdenii and Clostridium pasteurianum flavodoxins.","publicationDate":"1971","journal":"Biochemistry","firstPage":"3041","lastPage":"3046","volume":"10"},"referencePositions":["PROTEIN SEQUENCE OF 1-41 AND 136-137"],"referenceComments":[{"value":"ATCC 25940 / DSM 20460 / JCM 1772 / NCIB 8927","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"2161759","citationType":"journal article","authors":["van Mierlo C.P.M.","Mueller F.","Vervoort J."],"citationCrossReferences":[{"database":"PubMed","id":"2161759"},{"database":"DOI","id":"10.1111/j.1432-1033.1990.tb15527.x"}],"title":"Secondary and tertiary structure characteristics of Megasphaera elsdenii flavodoxin in the reduced state as determined by two-dimensional 1H NMR.","publicationDate":"1990","journal":"Eur. J. Biochem.","firstPage":"589","lastPage":"600","volume":"189"},"referencePositions":["STRUCTURE BY NMR"]},{"referenceNumber":5,"citation":{"id":"2253614","citationType":"journal article","authors":["van Mierlo C.P.M.","Lijnzaad P.","Vervoort J.","Mueller F.","Berendsen H.J.C.","de Vlieg J."],"citationCrossReferences":[{"database":"PubMed","id":"2253614"},{"database":"DOI","id":"10.1111/j.1432-1033.1990.tb19444.x"}],"title":"Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some implications, as determined by two-dimensional 1H-NMR and restrained molecular dynamics.","publicationDate":"1990","journal":"Eur. J. Biochem.","firstPage":"185","lastPage":"198","volume":"194"},"referencePositions":["STRUCTURE BY NMR"]}],"uniProtKBCrossReferences":[{"database":"PIR","id":"A92137","properties":[{"key":"EntryName","value":"FXME"}]},{"database":"RefSeq","id":"WP_014015302.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_AP031433.1"}]},{"database":"PDB","id":"2FZ5","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=1-137"}]},{"database":"AlphaFoldDB","id":"P00321","properties":[{"key":"Description","value":"-"}]},{"database":"BMRB","id":"P00321","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P00321","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"97491270","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9790745at2","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"P00321","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010181","properties":[{"key":"GoTerm","value":"F:FMN binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016651","properties":[{"key":"GoTerm","value":"F:oxidoreductase activity, acting on NAD(P)H"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"Gene3D","id":"3.40.50.360","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR010087","properties":[{"key":"EntryName","value":"Flav_short"}]},{"database":"InterPro","id":"IPR008254","properties":[{"key":"EntryName","value":"Flavodoxin/NO_synth"}]},{"database":"InterPro","id":"IPR001226","properties":[{"key":"EntryName","value":"Flavodoxin_CS"}]},{"database":"InterPro","id":"IPR029039","properties":[{"key":"EntryName","value":"Flavoprotein-like_sf"}]},{"database":"InterPro","id":"IPR051285","properties":[{"key":"EntryName","value":"NADH_oxidoreductase_modular"}]},{"database":"NCBIfam","id":"TIGR01753","properties":[{"key":"EntryName","value":"flav_short"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32145","properties":[{"key":"EntryName","value":"DIFLAVIN FLAVOPROTEIN A 2-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32145:SF11","properties":[{"key":"EntryName","value":"DIFLAVIN FLAVOPROTEIN A 2-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00258","properties":[{"key":"EntryName","value":"Flavodoxin_1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52218","properties":[{"key":"EntryName","value":"Flavoproteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00201","properties":[{"key":"EntryName","value":"FLAVODOXIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50902","properties":[{"key":"EntryName","value":"FLAVODOXIN_LIKE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVEIVYWSGTGNTEAMANEIEAAVKAAGADVESVRFEDTNVDDVASKDVILLGCPAMGSEELEDSVVEPFFTDLAPKLKGKKVGLFGSYGWGSGEWMDAWKQRTEDTGATVIGTAIVNEMPDNAPECKELGEAAAKA","length":137,"molWeight":14550,"crc64":"86BD744412D6F869","md5":"30EE2864EE34B8CD9E49EAA32B93DFD6"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Beta strand":6,"Helix":6,"Turn":1},"uniParcId":"UPI000012A8C3"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9ZIV1","uniProtkbId":"DNAK_MEGEL","entryAudit":{"firstPublicDate":"2002-11-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"1999-05-01","entryVersion":82,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Chaperone protein DnaK"}},"alternativeNames":[{"fullName":{"value":"HSP70"}},{"fullName":{"value":"Heat shock 70 kDa protein"}},{"fullName":{"value":"Heat shock protein 70"}}],"flag":"Fragment"},"genes":[{"geneName":{"value":"dnaK"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Acts as a chaperone"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"By stress conditions e.g. heat shock (By similarity)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the heat shock protein 70 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":450,"modifier":"OUTSIDE"}},"description":"Chaperone protein DnaK","featureId":"PRO_0000078485"},{"type":"Modified residue","location":{"start":{"value":174,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"Phosphothreonine; by autocatalysis","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Non-terminal residue","location":{"start":{"value":450,"modifier":"EXACT"},"end":{"value":450,"modifier":"EXACT"}},"description":""}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0143","category":"Molecular function","name":"Chaperone"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0346","category":"Biological process","name":"Stress response"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-6K0O4KCICSSEA","citationType":"submission","authors":["Gupta R.S."],"title":"The phylogeny of prokaryotes: a new proposal for the classification of organisms at the highest taxa level integrating phylogenetic and phenotypic characteristics.","publicationDate":"JUL-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 25940 / DSM 20460 / JCM 1772 / NCIB 8927","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF013113","properties":[{"key":"ProteinId","value":"AAC77524.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q9ZIV1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9ZIV1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016887","properties":[{"key":"GoTerm","value":"F:ATP hydrolysis activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd10234","properties":[{"key":"EntryName","value":"ASKHA_NBD_HSP70_DnaK-like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.420.40:FF:000071","properties":[{"key":"EntryName","value":"Molecular chaperone DnaK"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.640.10:FF:000003","properties":[{"key":"EntryName","value":"Molecular chaperone DnaK"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.420.40","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.90.640.10","properties":[{"key":"EntryName","value":"Actin, Chain A, domain 4"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.34.10","properties":[{"key":"EntryName","value":"Substrate Binding Domain Of DNAk, Chain A, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR043129","properties":[{"key":"EntryName","value":"ATPase_NBD"}]},{"database":"InterPro","id":"IPR018181","properties":[{"key":"EntryName","value":"Heat_shock_70_CS"}]},{"database":"InterPro","id":"IPR029047","properties":[{"key":"EntryName","value":"HSP70_peptide-bd_sf"}]},{"database":"InterPro","id":"IPR013126","properties":[{"key":"EntryName","value":"Hsp_70_fam"}]},{"database":"NCBIfam","id":"NF001413","properties":[{"key":"EntryName","value":"dnaK"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR19375","properties":[{"key":"EntryName","value":"HEAT SHOCK PROTEIN 70KDA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00012","properties":[{"key":"EntryName","value":"HSP70"},{"key":"MatchStatus","value":"2"}]},{"database":"PRINTS","id":"PR00301","properties":[{"key":"EntryName","value":"HEATSHOCK70"}]},{"database":"SUPFAM","id":"SSF53067","properties":[{"key":"EntryName","value":"Actin-like ATPase domain"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF100920","properties":[{"key":"EntryName","value":"Heat shock protein 70kD (HSP70), peptide-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00297","properties":[{"key":"EntryName","value":"HSP70_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00329","properties":[{"key":"EntryName","value":"HSP70_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01036","properties":[{"key":"EntryName","value":"HSP70_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSKVIGIDLGTTNSVVAVMEGGEPTVITNTEGSRLTPSVVGFSKTGERLVGQLAKRQAVSNPENTISSIKRHMGESYTVDIQGKKYTPQEISAMILQKLKEDAESYLGEKVTQAVITVPAYFNDGQRQATKDAGKIAGLDVLRIVNEPTAAALAYGLDKGGDGKILVFDLGGGTFDVSILELGDGVFEVKATNGNTHLGGDDFDNKVMEWMISEFKKETGIDLSQDKMAEQRLKEAAEKAKIELSTVLSTNINLPFITADATGPKHLDLTLTRAKFNELTEDLVQATMEPTKKAIADSGFTIDEIDKIILVGGSSRIPAVQEAIKSILGKEPSKGVNPDECVAIGAAIQAGVLVGDVKDVLLLDVTPLSLGIETLGGVFTKIIDRNTTIPTSRSQVFSTAVDNQPSVDVHVLQGRREMAADNKTLGRFELTGIPAAPRGVPRIEVTFNID","length":450,"molWeight":48067,"crc64":"2C337D32AA364F68","md5":"0549BFEAC212791656DCC91F06A8FFA1"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Modified residue":1,"Non-terminal residue":1},"uniParcId":"UPI00001295D8"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"C8C3X8","uniProtkbId":"C8C3X8_MEGEL","entryAudit":{"firstPublicDate":"2009-10-13","lastAnnotationUpdateDate":"2025-10-08","lastSequenceUpdateDate":"2009-10-13","entryVersion":60,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40899.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012967"},{"evidenceCode":"ECO:0000256","source":"NCBIfam","id":"TIGR01771"}],"value":"L-lactate dehydrogenase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012967"},{"evidenceCode":"ECO:0000256","source":"NCBIfam","id":"TIGR01771"}],"value":"1.1.1.27"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40899.1"}],"value":"ldhL"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(S)-lactate + NAD(+) = pyruvate + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23444"},{"database":"ChEBI","id":"CHEBI:15361"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16651"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"1.1.1.27","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049258"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004843"}],"value":"Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006054"}],"value":"Belongs to the LDH/MDH superfamily. LDH family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"Lactate/malate dehydrogenase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00056"}]},{"type":"Domain","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"Lactate/malate dehydrogenase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02866"}]},{"type":"Active site","location":{"start":{"value":157,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-1"}]},{"type":"Binding site","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":102,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40899.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":293,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40899.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-3"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003369"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"20680561","citationType":"journal article","authors":["Fecskeova L.","Piknova M.","Javorsky P.","Pristas P."],"citationCrossReferences":[{"database":"PubMed","id":"20680561"},{"database":"DOI","id":"10.1007/s12223-010-0048-z"}],"title":"Lactate dehydrogenase gene variability among predominant lactate utilizing ruminal bacteria.","publicationDate":"2010","journal":"Folia Microbiol. (Praha)","firstPage":"315","lastPage":"318","volume":"55"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40899.1"}],"value":"2T","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40901.1"}],"value":"4O","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40899.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"GQ365641","properties":[{"key":"ProteinId","value":"ACU40899.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GQ365643","properties":[{"key":"ProteinId","value":"ACU40901.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"C8C3X8","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00554","properties":[{"key":"RectionId","value":"UER00611"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004459","properties":[{"key":"GoTerm","value":"F:L-lactate dehydrogenase (NAD+) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006089","properties":[{"key":"GoTerm","value":"P:lactate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd05290","properties":[{"key":"EntryName","value":"LDH_3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.110.10","properties":[{"key":"EntryName","value":"Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001557","properties":[{"key":"EntryName","value":"L-lactate/malate_DH"}]},{"database":"InterPro","id":"IPR011304","properties":[{"key":"EntryName","value":"L-lactate_DH"}]},{"database":"InterPro","id":"IPR022383","properties":[{"key":"EntryName","value":"Lactate/malate_DH_C"}]},{"database":"InterPro","id":"IPR001236","properties":[{"key":"EntryName","value":"Lactate/malate_DH_N"}]},{"database":"InterPro","id":"IPR015955","properties":[{"key":"EntryName","value":"Lactate_DH/Glyco_Ohase_4_C"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"NCBIfam","id":"TIGR01771","properties":[{"key":"EntryName","value":"L-LDH-NAD"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43128","properties":[{"key":"EntryName","value":"L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43128:SF16","properties":[{"key":"EntryName","value":"L-LACTATE DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02866","properties":[{"key":"EntryName","value":"Ldh_1_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00056","properties":[{"key":"EntryName","value":"Ldh_1_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000102","properties":[{"key":"EntryName","value":"Lac_mal_DH"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00086","properties":[{"key":"EntryName","value":"LLDHDRGNASE"}]},{"database":"SUPFAM","id":"SSF56327","properties":[{"key":"EntryName","value":"LDH C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"ADFQLATEVVLIDLNEDKAWGEAKDSSHATSCIYSTNIKFHLGDYEDCKDANIIVITAGPSIRPGETPDRLKLAGTNAKIMSSVMGEIVKRTKEAMIIMITNPLDVATYVVSTQFDYPRNLILGTGTMLETYRFRRILADKYQVDPKNINGYVLGEHGNAAFVAWSTTGCAGFPIDDLDEYFHRTEKLSHEAVEQELVQVAYDVINKKGFTNTGIAMAACRFIKSVLYDEHTILPCSAVLEGEYGIKDVALSIPRMVCADGIMRSFEVHLTDDELEKMHKAAQSVRSALDGAG","length":293,"molWeight":32340,"crc64":"E7C4A7B532663952","md5":"038B8FC4DA593457172F591A9757F519"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Active site":1,"Binding site":3,"Non-terminal residue":2},"uniParcId":"UPI0001B1E899"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"C8C3X9","uniProtkbId":"C8C3X9_MEGEL","entryAudit":{"firstPublicDate":"2009-10-13","lastAnnotationUpdateDate":"2025-10-08","lastSequenceUpdateDate":"2009-10-13","entryVersion":57,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40900.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012967"},{"evidenceCode":"ECO:0000256","source":"NCBIfam","id":"TIGR01771"}],"value":"L-lactate dehydrogenase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012967"},{"evidenceCode":"ECO:0000256","source":"NCBIfam","id":"TIGR01771"}],"value":"1.1.1.27"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40900.1"}],"value":"ldhL"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(S)-lactate + NAD(+) = pyruvate + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23444"},{"database":"ChEBI","id":"CHEBI:15361"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16651"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"1.1.1.27","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049258"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004843"}],"value":"Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006054"}],"value":"Belongs to the LDH/MDH superfamily. LDH family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"Lactate/malate dehydrogenase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00056"}]},{"type":"Domain","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"Lactate/malate dehydrogenase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02866"}]},{"type":"Active site","location":{"start":{"value":157,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-1"}]},{"type":"Binding site","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":102,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40900.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":293,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40900.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000102-3"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003369"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"20680561","citationType":"journal article","authors":["Fecskeova L.","Piknova M.","Javorsky P.","Pristas P."],"citationCrossReferences":[{"database":"PubMed","id":"20680561"},{"database":"DOI","id":"10.1007/s12223-010-0048-z"}],"title":"Lactate dehydrogenase gene variability among predominant lactate utilizing ruminal bacteria.","publicationDate":"2010","journal":"Folia Microbiol. (Praha)","firstPage":"315","lastPage":"318","volume":"55"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40900.1"}],"value":"3J","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACU40900.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"GQ365642","properties":[{"key":"ProteinId","value":"ACU40900.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"C8C3X9","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00554","properties":[{"key":"RectionId","value":"UER00611"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004459","properties":[{"key":"GoTerm","value":"F:L-lactate dehydrogenase (NAD+) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006089","properties":[{"key":"GoTerm","value":"P:lactate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd05290","properties":[{"key":"EntryName","value":"LDH_3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.110.10","properties":[{"key":"EntryName","value":"Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001557","properties":[{"key":"EntryName","value":"L-lactate/malate_DH"}]},{"database":"InterPro","id":"IPR011304","properties":[{"key":"EntryName","value":"L-lactate_DH"}]},{"database":"InterPro","id":"IPR022383","properties":[{"key":"EntryName","value":"Lactate/malate_DH_C"}]},{"database":"InterPro","id":"IPR001236","properties":[{"key":"EntryName","value":"Lactate/malate_DH_N"}]},{"database":"InterPro","id":"IPR015955","properties":[{"key":"EntryName","value":"Lactate_DH/Glyco_Ohase_4_C"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"NCBIfam","id":"TIGR01771","properties":[{"key":"EntryName","value":"L-LDH-NAD"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43128","properties":[{"key":"EntryName","value":"L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43128:SF16","properties":[{"key":"EntryName","value":"L-LACTATE DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02866","properties":[{"key":"EntryName","value":"Ldh_1_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00056","properties":[{"key":"EntryName","value":"Ldh_1_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000102","properties":[{"key":"EntryName","value":"Lac_mal_DH"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00086","properties":[{"key":"EntryName","value":"LLDHDRGNASE"}]},{"database":"SUPFAM","id":"SSF56327","properties":[{"key":"EntryName","value":"LDH C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"SDFQLATEVVLIDLNEDKAWGEAKDSSHATSCIYSTNIKFHLGDYEDCKDANIIVITAGPSIRPGETPDRLKLAGTNAKIMSSVMGEIVKRTKEAMIIMITNPLDVATYVVSTQFDYPRNLILGTGTMLETYRFRRILADKYQVDPKNINGYVLGEHGNAAFVAWSTTGCAGFPIDDLDEYFHRTEKLSHEAVEQELVQVAYDVINKKGFTNTGIAMAACRFIKSVLYDEHTILPCSAVLEGEYGIKDVALSIPRMVCADGIMRSFEVHLTDDELEKMHKAAQSVRSALDGAG","length":293,"molWeight":32356,"crc64":"6D927CD0D461A72D","md5":"65B858517F83DBFB43528B324AB3F10F"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Active site":1,"Binding site":3,"Non-terminal residue":2},"uniParcId":"UPI0001B1E89A"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"F6LNL3","uniProtkbId":"F6LNL3_MEGEL","entryAudit":{"firstPublicDate":"2011-07-27","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2011-07-27","entryVersion":54,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEB33909.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEB33909.1"}],"value":"Putative ABC transporter"}}]},"comments":[{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004202"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004202"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005417"}],"value":"Belongs to the ABC transporter superfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"ABC transporter","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50893"}]},{"type":"Binding site","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00434"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00434"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022519"}],"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022475"}],"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00434"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"21821757","citationType":"journal article","authors":["Stanton T.B.","Humphrey S.B."],"citationCrossReferences":[{"database":"PubMed","id":"21821757"},{"database":"DOI","id":"10.1128/AEM.00647-11"}],"title":"Persistence of Antibiotic Resistance: Evaluation of a Probiotic Approach Using Antibiotic-Sensitive Megasphaera elsdenii Strains To Prevent Colonization of Swine by Antibiotic-Resistant Strains.","publicationDate":"2011","journal":"Appl. Environ. Microbiol.","firstPage":"7158","lastPage":"7166","volume":"77"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEB33909.1"}],"value":"14-14","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEB33909.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"JF297584","properties":[{"key":"ProteinId","value":"AEB33909.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_054335829.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CAMDYL010000001.1"}]},{"database":"AlphaFoldDB","id":"F6LNL3","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016887","properties":[{"key":"GoTerm","value":"F:ATP hydrolysis activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0015833","properties":[{"key":"GoTerm","value":"P:peptide transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd03257","properties":[{"key":"EntryName","value":"ABC_NikE_OppD_transporters"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000016","properties":[{"key":"EntryName","value":"Oligopeptide ABC transporter ATP-binding component"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003593","properties":[{"key":"EntryName","value":"AAA+_ATPase"}]},{"database":"InterPro","id":"IPR050388","properties":[{"key":"EntryName","value":"ABC_Ni/Peptide_Import"}]},{"database":"InterPro","id":"IPR003439","properties":[{"key":"EntryName","value":"ABC_transporter-like_ATP-bd"}]},{"database":"InterPro","id":"IPR013563","properties":[{"key":"EntryName","value":"Oligopep_ABC_C"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"NCBIfam","id":"TIGR01727","properties":[{"key":"EntryName","value":"oligo_HPY"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43297:SF14","properties":[{"key":"EntryName","value":"ATPASE AAA-TYPE CORE DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43297","properties":[{"key":"EntryName","value":"OLIGOPEPTIDE TRANSPORT ATP-BINDING PROTEIN APPD"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00005","properties":[{"key":"EntryName","value":"ABC_tran"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08352","properties":[{"key":"EntryName","value":"oligo_HPY"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00382","properties":[{"key":"EntryName","value":"AAA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50893","properties":[{"key":"EntryName","value":"ABC_TRANSPORTER_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MQALLEVRHLTISFHTYRGLLEAIHDVSLTVNPGETVGIVGESGCGKSVTSQAAMKLLPAEATEYTSGQILWDGRDVIPLSEQKMNHLRGRDMAMIFQDPMTSLNPVLTIGTQLCEGIALHQHLGRKACQAKALELLKAVGLTSPEKRLHQYPHELSGGMRQRCLIAMALSCSPRLLFADEPTTALDVTTEAQVLDVLKRLQAELGMAVVLISHNLGVIAQMCRRIYVMYAGVVVEEGTADDIFYRPAHPYTKGLLASLPDPSQKDKALTGIPGQAPDLFHMPQGCRFYPRCPRAMKACMEAAPPREEAGPGHYYRCWLAQAAAAKEALP","length":330,"molWeight":36079,"crc64":"A6EAF7C741529D8C","md5":"DAD56CD8FD86C3F3762EAA49614FB5D7"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Binding site":1},"uniParcId":"UPI000211A150"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"F6LNL4","uniProtkbId":"F6LNL4_MEGEL","entryAudit":{"firstPublicDate":"2011-07-27","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-07-27","entryVersion":32,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEB33910.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEB33910.1"}],"value":"Putative 2-hydroxyglutaryl-CoA dehydratase subunit"}}]},"comments":[{"commentType":"COFACTOR","cofactors":[{"name":"[4Fe-4S] cluster","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR610327-1"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49883"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR610327-1"}],"value":"Binds 1 [4Fe-4S] cluster."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005806"}],"value":"Belongs to the FldB/FldC dehydratase alpha/beta subunit family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Binding site","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR610327-1"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR610327-1"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":366,"modifier":"EXACT"},"end":{"value":366,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR610327-1"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR610327-1"}],"id":"KW-0004","category":"Ligand","name":"4Fe-4S"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR610327-1"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023014"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR610327-1"}],"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR610327-1"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"21821757","citationType":"journal article","authors":["Stanton T.B.","Humphrey S.B."],"citationCrossReferences":[{"database":"PubMed","id":"21821757"},{"database":"DOI","id":"10.1128/AEM.00647-11"}],"title":"Persistence of Antibiotic Resistance: Evaluation of a Probiotic Approach Using Antibiotic-Sensitive Megasphaera elsdenii Strains To Prevent Colonization of Swine by Antibiotic-Resistant Strains.","publicationDate":"2011","journal":"Appl. Environ. Microbiol.","firstPage":"7158","lastPage":"7166","volume":"77"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEB33910.1"}],"value":"14-14","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEB33910.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"JF297585","properties":[{"key":"ProteinId","value":"AEB33910.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_014015708.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CALDUZ010000054.1"}]},{"database":"AlphaFoldDB","id":"F6LNL4","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"97491703","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0016836","properties":[{"key":"GoTerm","value":"F:hydro-lyase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"Gene3D","id":"3.40.50.11890","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.11900","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR010327","properties":[{"key":"EntryName","value":"FldB/FldC_alpha/beta"}]},{"database":"PANTHER","id":"PTHR30548","properties":[{"key":"EntryName","value":"2-HYDROXYGLUTARYL-COA DEHYDRATASE, D-COMPONENT-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30548:SF4","properties":[{"key":"EntryName","value":"SUBUNIT OF OXYGEN-SENSITIVE 2-HYDROXYISOCAPROYL-COA DEHYDRATASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06050","properties":[{"key":"EntryName","value":"HGD-D"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSEEKTVDIESMSSKEALGYFLPKVDEDARKAKKEGRLVCWSASVAPPEFCTAMDIAIVYPETHAAGIGARHGAPAMLEVAENKGYNQDICSYCRVNMGYMELLKQQALTGETPEVLKNSPASPIPLPDVVLTCNNICNTLLKWYENLAKELNVPLINIDVPFNHEFPVTKHAKQYIVGEFKHAIKQLEDLCGRPFDYDKFFEVQKQTQRSIAAWNKIATYFQYKPSPLNGFDLFNYMGLAVAARSLNYSEITFNKFLKELDEKVANKKWAFGENEKSRVTWEGIAVWIALGHTFKELKGQGALMTGSAYPGMWDVSYEPGDLESMAEAYSRTYINCCLEQRGAVLEKVVRDGKCDGLIMHQNRSCKNMSLLNNEGGQRIQKNLGVPYVIFDGDQTDARNFSEAQFDTRVEALAEMMADKKANEGGNH","length":428,"molWeight":48089,"crc64":"F065C0E48BE1933D","md5":"675A1838562933407374B482F8937752"},"extraAttributes":{"countByCommentType":{"COFACTOR":1,"SIMILARITY":1},"countByFeatureType":{"Binding site":3},"uniParcId":"UPI00004FADD5"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q0ZQJ4","uniProtkbId":"Q0ZQJ4_MEGEL","entryAudit":{"firstPublicDate":"2006-08-22","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-02-26","entryVersion":52,"sequenceVersion":2},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69358.4"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"4: Predicted","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69358.4"}],"value":"PTP-like phytase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69358.4"}],"value":"phyA"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10684"},{"database":"Rhea","id":"RHEA-COMP:10136"},{"database":"Rhea","id":"RHEA-COMP:20101"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:46858"},{"database":"ChEBI","id":"CHEBI:61978"}],"ecNumber":"3.1.3.48","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10044"}]}}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":360,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5052591922"},{"type":"Domain","location":{"start":{"value":245,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"Tyrosine specific protein phosphatases","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50056"}]},{"type":"Active site","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Phosphocysteine intermediate","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10044"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-7M1RJAGTFAEDJ","citationType":"submission","authors":["Puhl A.A.","Selinger L.B.","Greiner R."],"title":"Cloning and characterization of a novel PTP-like phytase from Megasphaera elsdenii with mixed 3- or 6-phosphate position specificity.","publicationDate":"DEC-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69358.4"}],"value":"YR60","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69358.4"}]},{"referenceNumber":2,"citation":{"id":"17186149","citationType":"journal article","authors":["Nakashima B.A.","McAllister T.A.","Sharma R.","Selinger L.B."],"citationCrossReferences":[{"database":"PubMed","id":"17186149"},{"database":"DOI","id":"10.1007/s00248-006-9147-4"}],"title":"Diversity of phytases in the rumen.","publicationDate":"2007","journal":"Microb. Ecol.","firstPage":"82","lastPage":"88","volume":"53"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69358.4"}],"value":"YR60","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69358.4"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DQ257441","properties":[{"key":"ProteinId","value":"ABC69358.4"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q0ZQJ4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0004725","properties":[{"key":"GoTerm","value":"F:protein tyrosine phosphatase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"CDD","id":"cd14495","properties":[{"key":"EntryName","value":"PTPLP-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.1690","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.190.10","properties":[{"key":"EntryName","value":"Protein tyrosine phosphatase superfamily"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR029021","properties":[{"key":"EntryName","value":"Prot-tyrosine_phosphatase-like"}]},{"database":"InterPro","id":"IPR016130","properties":[{"key":"EntryName","value":"Tyr_Pase_AS"}]},{"database":"InterPro","id":"IPR000387","properties":[{"key":"EntryName","value":"Tyr_Pase_dom"}]},{"database":"Pfam","id":"PF14566","properties":[{"key":"EntryName","value":"PTPlike_phytase"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01301","properties":[{"key":"EntryName","value":"PTPlike_phytase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52799","properties":[{"key":"EntryName","value":"(Phosphotyrosine protein) phosphatases II"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00383","properties":[{"key":"EntryName","value":"TYR_PHOSPHATASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50056","properties":[{"key":"EntryName","value":"TYR_PHOSPHATASE_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MHKNKLLQSKGKIMIRCKKIAVILAVFSAMGMVVQGQGNGDNIAFSQGGRTPLPALAPAAYEGHFWRVDAENDASLPRNFRTCQSPFHAVEHKYASEVDPSYIPSRKGLDDLRISGSSQFSARQFDALVHELRKKTKGPIYDVDLRQESHGFFDGTAVSWYGRHDWGNIGKSPTAVLADEQQRLQAALGKDVIVYDQGKGDLPIHPRVIAVRRVQTEQELAESKGIHYVRLANTDHLWPTPGEIDAFLAFVRTLPADAWLHFHCEAGAGRTTAYMVMYDMIKNPDLPYKDIVYRQYEIGGNYTPHDVVHPKQGDWKGPYYHEKHEMVSLFYQYVQDQTKQRWSQSWSQWLENKRMRVNNR","length":360,"molWeight":41115,"crc64":"94AA03F57EA44959","md5":"59E968C84BE2B9604ACC65DAE0FF0722"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":1},"uniParcId":"UPI0001630E79"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q6RZ33","uniProtkbId":"Q6RZ33_MEGEL","entryAudit":{"firstPublicDate":"2004-07-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":103,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29973.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29973.1"}],"value":"Mosaic tetracycline-resistance protein"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29973.1"}],"value":"tetOW"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"Tr-type G","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51722"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022917"}],"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"15184185","citationType":"journal article","authors":["Stanton T.B.","McDowall J.S.","Rasmussen M.A."],"citationCrossReferences":[{"database":"PubMed","id":"15184185"},{"database":"DOI","id":"10.1128/AEM.70.6.3754-3757.2004"}],"title":"Diverse tetracycline resistance genotypes of Megasphaera elsdenii strains selectively cultured from swine feces.","publicationDate":"2004","journal":"Appl. Environ. Microbiol.","firstPage":"3754","lastPage":"3757","volume":"70"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29969.1"}],"value":"25-51","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29973.1"}],"value":"27-51","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29973.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY485122","properties":[{"key":"ProteinId","value":"AAR29969.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY485126","properties":[{"key":"ProteinId","value":"AAR29973.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_204313008.1","properties":[{"key":"NucleotideSequenceId","value":"NG_073451.1"}]},{"database":"AlphaFoldDB","id":"Q6RZ33","properties":[{"key":"Description","value":"-"}]},{"database":"CARD","id":"ARO:3007121","properties":[{"key":"CARD short name","value":"tet(O/W)"},{"key":"Resistance mechanism identifier","value":"ARO:0001003"},{"key":"Resistance mechanism name","value":"antibiotic target protection"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd03711","properties":[{"key":"EntryName","value":"Tet_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03690","properties":[{"key":"EntryName","value":"Tet_II"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16258","properties":[{"key":"EntryName","value":"Tet_III"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01684","properties":[{"key":"EntryName","value":"Tet_like_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04168","properties":[{"key":"EntryName","value":"TetM_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.870","properties":[{"key":"EntryName","value":"Elongation Factor G (Translational Gtpase), domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR041095","properties":[{"key":"EntryName","value":"EFG_II"}]},{"database":"InterPro","id":"IPR035647","properties":[{"key":"EntryName","value":"EFG_III/V"}]},{"database":"InterPro","id":"IPR000640","properties":[{"key":"EntryName","value":"EFG_V-like"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR005225","properties":[{"key":"EntryName","value":"Small_GTP-bd"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR035650","properties":[{"key":"EntryName","value":"Tet_C"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR005517","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2_IV"}]},{"database":"NCBIfam","id":"TIGR00231","properties":[{"key":"EntryName","value":"small_GTP"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF012153","properties":[{"key":"EntryName","value":"tet_protect"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033148","properties":[{"key":"EntryName","value":"tet_protect_M_W"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261:SF1","properties":[{"key":"EntryName","value":"RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261","properties":[{"key":"EntryName","value":"TRANSLATION ELONGATION FACTOR G-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00679","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14492","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03764","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"PRINTS","id":"PR01037","properties":[{"key":"EntryName","value":"TCRTETOQM"}]},{"database":"SMART","id":"SM00889","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54980","properties":[{"key":"EntryName","value":"EF-G C-terminal domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKIINLGILAHVDAGKTTLTESLLYTSGAIAELGSVDEGTTRTDTMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLAVLDGAILVISAKDGVQAQTRILFHALRKMNIPTVIFINKIDQAGVDLQSVVQSVRDKLSADIIIKQTVSLSPEIVLEENTDIEAWDAVIENNDALLEKYIAGEPISQEKLAREEQRRVQEASLFPVYHGSAKKGLGIQPLMDAVTGLFQPIGEQGSATLCGSVFKVEYTDCGQRRVYLRLYSGTLRLRDTVALAGREKLKITEMRIPSKGEIVRTDTAYPGEIVILPSDSVRLNDVLGDQTRLPRKRWREAPLPMLRTTIAPKTAAQRERLLDALTQLADTDPLLRCEVDSITHEIILSFLGRVQLEVVSALLSEKYKIETVVKEPTVIYMERPLKAASHTIHIEVPPNPFWASIGLSVTPLPLGSGVQYESRVSLGYLNQSFQNAVRDGIRYGLEQGLFGWNVTDCKICFEYGLYYSPVSTPADFRSLAPIVLEQALKESGTQLLEPYLSFTLYAPQEYLSRAYHDAPKYCATIETAQVKKDEVVFTGEIPARCIQAYRTDLAFYTNGQSVCLTELKGYQAAVGQPVIQPRRPNNRLDKVRHMFQKVM","length":639,"molWeight":71236,"crc64":"B98C66E2A91CA3B3","md5":"0E715AB50198F27F2FFDB38B9F6B52F5"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1},"uniParcId":"UPI00002313E0"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q6RZ34","uniProtkbId":"Q6RZ34_MEGEL","entryAudit":{"firstPublicDate":"2004-07-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":93,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29972.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29972.1"}],"value":"Tetracycline resistance protein"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29972.1"}],"value":"tetW"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"Tr-type G","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51722"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022917"}],"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"15184185","citationType":"journal article","authors":["Stanton T.B.","McDowall J.S.","Rasmussen M.A."],"citationCrossReferences":[{"database":"PubMed","id":"15184185"},{"database":"DOI","id":"10.1128/AEM.70.6.3754-3757.2004"}],"title":"Diverse tetracycline resistance genotypes of Megasphaera elsdenii strains selectively cultured from swine feces.","publicationDate":"2004","journal":"Appl. Environ. Microbiol.","firstPage":"3754","lastPage":"3757","volume":"70"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29972.1"}],"value":"25-50","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29972.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY485125","properties":[{"key":"ProteinId","value":"AAR29972.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_063856416.1","properties":[{"key":"NucleotideSequenceId","value":"NG_048287.1"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd03711","properties":[{"key":"EntryName","value":"Tet_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03690","properties":[{"key":"EntryName","value":"Tet_II"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16258","properties":[{"key":"EntryName","value":"Tet_III"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01684","properties":[{"key":"EntryName","value":"Tet_like_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04168","properties":[{"key":"EntryName","value":"TetM_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.870","properties":[{"key":"EntryName","value":"Elongation Factor G (Translational Gtpase), domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR041095","properties":[{"key":"EntryName","value":"EFG_II"}]},{"database":"InterPro","id":"IPR035647","properties":[{"key":"EntryName","value":"EFG_III/V"}]},{"database":"InterPro","id":"IPR000640","properties":[{"key":"EntryName","value":"EFG_V-like"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR005225","properties":[{"key":"EntryName","value":"Small_GTP-bd"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR035650","properties":[{"key":"EntryName","value":"Tet_C"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR005517","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2_IV"}]},{"database":"NCBIfam","id":"TIGR00231","properties":[{"key":"EntryName","value":"small_GTP"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF012153","properties":[{"key":"EntryName","value":"tet_protect"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033148","properties":[{"key":"EntryName","value":"tet_protect_M_W"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261:SF1","properties":[{"key":"EntryName","value":"RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261","properties":[{"key":"EntryName","value":"TRANSLATION ELONGATION FACTOR G-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00679","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14492","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03764","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"PRINTS","id":"PR01037","properties":[{"key":"EntryName","value":"TCRTETOQM"}]},{"database":"SMART","id":"SM00889","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54980","properties":[{"key":"EntryName","value":"EF-G C-terminal domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKIINIGILAHVDAGKTTLTESLLYASGAISEPGSVEKGTTRTDTMFLERQRGITIQAAVTSFQWHRCKVNIVDTPGHMDFLAEVYRSLAVLDGAILVISAKDGVQAQTRILFHALRKMNIPTVIFINKIDQAGVDLQSVVQSVRDKLSADIIIKQTVSLSPEIVLEENTDIEAWDAVIENNDELLEKYIAGEPISREKLAREEQQRVQDASLFPVYHGSAKNGLGIQPLMDAVTGLFQPIGEQGGAALCGSVFKVEYTDCGQRRVYLRLYSGTLRLRDTVALAGREKLKITEMRIPSKGEIVRTDTAYQGEIVILPSDSVRLNDVLGDQTRLPRKRWREDPLPMLRTTIAPKTAAQRERLLDALTQLADTDPLLRCEVDSITHEIILSFLGRVQLEVVSALLSEKYKLETVVKEPSVIYMERPLKAASHTIHIEVPPNPFWASIGLSVTPLSLGSGVQYESRVSLGYLNQSFQNAVRDGIRYGLEQGLFGWXVTDCKICFEYGLYYSPVSTPADFRSLAPIVLEQALKESGTQLLEPYLSFILYAPQEYLSRAYHDAPKYCATIETAQVKKDEVVFTGEIPARCIQAYRTDLAFYTNGRSVCLTELKGYQAAVGQPVIQPRRPNSRLDKVRHMFQKVM","length":639,"molWeight":71292,"crc64":"87228898F6A318B5","md5":"74443151746799917791BBCFB011C980"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1},"uniParcId":"UPI00002313E3"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q6RZ35","uniProtkbId":"Q6RZ35_MEGEL","entryAudit":{"firstPublicDate":"2004-07-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":96,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29971.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29971.1"}],"value":"Tetracycline resistance protein"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29971.1"}],"value":"tetW"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"Tr-type G","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51722"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022917"}],"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"15184185","citationType":"journal article","authors":["Stanton T.B.","McDowall J.S.","Rasmussen M.A."],"citationCrossReferences":[{"database":"PubMed","id":"15184185"},{"database":"DOI","id":"10.1128/AEM.70.6.3754-3757.2004"}],"title":"Diverse tetracycline resistance genotypes of Megasphaera elsdenii strains selectively cultured from swine feces.","publicationDate":"2004","journal":"Appl. Environ. Microbiol.","firstPage":"3754","lastPage":"3757","volume":"70"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29971.1"}],"value":"29-55","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29971.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY485124","properties":[{"key":"ProteinId","value":"AAR29971.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_063856415.1","properties":[{"key":"NucleotideSequenceId","value":"NG_048286.1"}]},{"database":"AlphaFoldDB","id":"Q6RZ35","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd03711","properties":[{"key":"EntryName","value":"Tet_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03690","properties":[{"key":"EntryName","value":"Tet_II"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16258","properties":[{"key":"EntryName","value":"Tet_III"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01684","properties":[{"key":"EntryName","value":"Tet_like_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04168","properties":[{"key":"EntryName","value":"TetM_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.870","properties":[{"key":"EntryName","value":"Elongation Factor G (Translational Gtpase), domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR041095","properties":[{"key":"EntryName","value":"EFG_II"}]},{"database":"InterPro","id":"IPR035647","properties":[{"key":"EntryName","value":"EFG_III/V"}]},{"database":"InterPro","id":"IPR000640","properties":[{"key":"EntryName","value":"EFG_V-like"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR005225","properties":[{"key":"EntryName","value":"Small_GTP-bd"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR035650","properties":[{"key":"EntryName","value":"Tet_C"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR005517","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2_IV"}]},{"database":"NCBIfam","id":"TIGR00231","properties":[{"key":"EntryName","value":"small_GTP"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF012153","properties":[{"key":"EntryName","value":"tet_protect"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033148","properties":[{"key":"EntryName","value":"tet_protect_M_W"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261:SF1","properties":[{"key":"EntryName","value":"RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261","properties":[{"key":"EntryName","value":"TRANSLATION ELONGATION FACTOR G-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00679","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14492","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03764","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"PRINTS","id":"PR01037","properties":[{"key":"EntryName","value":"TCRTETOQM"}]},{"database":"SMART","id":"SM00889","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54980","properties":[{"key":"EntryName","value":"EF-G C-terminal domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKIINIGILAHVDAGKTTLTESLLYASGAISEPGSVEKGTTRTDTMFLERQRGITIQAAVTSFQWHRCKVNIVDTPGHMDFLAEVYRSLAVLDGAILVISAKDGVQAQTRILFHALRKMNIPTVIFINKIDQAGVDLQSVVQSVRDKLSADIIIKQTVSLSPEIVLEENTDIEAWDAVIENNDALLEKYIAGEPISQEKLAREEQRRVQEASLFPVYHGSAKKGLGIQPLMDAVTGLFQPIGEQGSATLCGSVFKVEYTDCGQRRVYLRLYSGTLRLRDTVALAGREKLKITEMRIPSKGEIVRTDTAYPGEIVILPSDSVRLNDVLGDQTRLPRKRWREAPLPMLRTTIAPKTAAQRERLLDALTQLADTDPLLRCEVDSITHEIILSFLGRVQLEVVSALLSEKYKIETVVKEPTVIYMERPLKAASHTIHIEVPPNPFWASIGLSVTPLPLGSGVQYESRVSLGYLNQSFQNAVRDGIRYGLEQGLFGWNVTDCKICFEYGLYYSPVSTPADFRSLAPIVLEQALKESGTQLLEPYLSFTLYAPQEYLSRAYHDAPKYCATIETAQVKKDEVVFTGEIPARCIQAYRTDLAFYTNGQSVCLTELKGYQAAVGQPVIQPRRPNNRLDKVRHMFQKVM","length":639,"molWeight":71261,"crc64":"D431BA92977450EC","md5":"49D3D2E14881F479B885CEB3A1B041B6"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1},"uniParcId":"UPI00002313E2"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q6RZ36","uniProtkbId":"Q6RZ36_MEGEL","entryAudit":{"firstPublicDate":"2004-07-05","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2004-07-05","entryVersion":99,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29970.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29970.1"}],"value":"Tetracycline resistance protein"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29970.1"}],"value":"tetO"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003987"}],"value":"Abolishes the inhibitory effect of tetracyclin on protein synthesis by a non-covalent modification of the ribosomes"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":244,"modifier":"EXACT"}},"description":"Tr-type G","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51722"}]},{"type":"Region","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"G1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"G2","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"G3","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"G4","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"G5","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022917"}],"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"15184185","citationType":"journal article","authors":["Stanton T.B.","McDowall J.S.","Rasmussen M.A."],"citationCrossReferences":[{"database":"PubMed","id":"15184185"},{"database":"DOI","id":"10.1128/AEM.70.6.3754-3757.2004"}],"title":"Diverse tetracycline resistance genotypes of Megasphaera elsdenii strains selectively cultured from swine feces.","publicationDate":"2004","journal":"Appl. Environ. Microbiol.","firstPage":"3754","lastPage":"3757","volume":"70"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29970.1"}],"value":"Fern","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAR29970.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY485123","properties":[{"key":"ProteinId","value":"AAR29970.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_050824634.1","properties":[{"key":"NucleotideSequenceId","value":"NG_048258.1"}]},{"database":"AlphaFoldDB","id":"Q6RZ36","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd03711","properties":[{"key":"EntryName","value":"Tet_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03690","properties":[{"key":"EntryName","value":"Tet_II"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16258","properties":[{"key":"EntryName","value":"Tet_III"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01684","properties":[{"key":"EntryName","value":"Tet_like_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04168","properties":[{"key":"EntryName","value":"TetM_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.870","properties":[{"key":"EntryName","value":"Elongation Factor G (Translational Gtpase), domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR053905","properties":[{"key":"EntryName","value":"EF-G-like_DII"}]},{"database":"InterPro","id":"IPR041095","properties":[{"key":"EntryName","value":"EFG_II"}]},{"database":"InterPro","id":"IPR035647","properties":[{"key":"EntryName","value":"EFG_III/V"}]},{"database":"InterPro","id":"IPR000640","properties":[{"key":"EntryName","value":"EFG_V-like"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR005225","properties":[{"key":"EntryName","value":"Small_GTP-bd"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR035650","properties":[{"key":"EntryName","value":"Tet_C"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR005517","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2_IV"}]},{"database":"NCBIfam","id":"TIGR00231","properties":[{"key":"EntryName","value":"small_GTP"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF012153","properties":[{"key":"EntryName","value":"tet_protect"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033148","properties":[{"key":"EntryName","value":"tet_protect_M_W"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261:SF1","properties":[{"key":"EntryName","value":"RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261","properties":[{"key":"EntryName","value":"TRANSLATION ELONGATION FACTOR G-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22042","properties":[{"key":"EntryName","value":"EF-G_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00679","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14492","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03764","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"PRINTS","id":"PR01037","properties":[{"key":"EntryName","value":"TCRTETOQM"}]},{"database":"SUPFAM","id":"SSF54980","properties":[{"key":"EntryName","value":"EF-G C-terminal domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKIINLGILGHVDAGKTTLTESLLYTSGAIAELGSVDEGTTRTDTMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAKDGIQAQTRILFHALQIMKIPTIFFINKIDQEGIDLPMVYREMKAKLSSEIIVKQKVGQHPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYHGSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLHLRDVIRISEKEKIKITEMYVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEILLPQRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLGNVQMEVICAILEEKYHVEAEIKEPTVIYMERPLRKAEYTIHIEVPPNPFWASVGLSIEPLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYSPVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIVSTQIKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRPNSRIDKVRHMFHKLA","length":639,"molWeight":72515,"crc64":"C817DF2B087CA4E6","md5":"7A9BFF2CD4547AE7B2E11EFDBFAD99FB"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Region":5},"uniParcId":"UPI00002313E1"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q84FH0","uniProtkbId":"Q84FH0_MEGEL","entryAudit":{"firstPublicDate":"2003-06-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2003-06-01","entryVersion":102,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42741.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42741.1"}],"value":"Tetracycline resistance protein"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42741.1"}],"value":"tet"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"Tr-type G","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51722"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42741.1"}],"id":"KW-0195","category":"Molecular function","name":"Cyclin"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022917"}],"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"12839756","citationType":"journal article","authors":["Stanton T.B.","Humphrey S.B."],"citationCrossReferences":[{"database":"PubMed","id":"12839756"},{"database":"DOI","id":"10.1128/AEM.69.7.3874-3882.2003"}],"title":"Isolation of tetracycline-resistant Megasphaera elsdenii strains with novel mosaic gene combinations of tet(O) and tet(W) from swine.","publicationDate":"2003","journal":"Appl. Environ. Microbiol.","firstPage":"3874","lastPage":"3882","volume":"69"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42741.1"}],"value":"7-11","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42741.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY196921","properties":[{"key":"ProteinId","value":"AAO42741.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q84FH0","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd03711","properties":[{"key":"EntryName","value":"Tet_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03690","properties":[{"key":"EntryName","value":"Tet_II"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16258","properties":[{"key":"EntryName","value":"Tet_III"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01684","properties":[{"key":"EntryName","value":"Tet_like_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04168","properties":[{"key":"EntryName","value":"TetM_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.870","properties":[{"key":"EntryName","value":"Elongation Factor G (Translational Gtpase), domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR041095","properties":[{"key":"EntryName","value":"EFG_II"}]},{"database":"InterPro","id":"IPR035647","properties":[{"key":"EntryName","value":"EFG_III/V"}]},{"database":"InterPro","id":"IPR000640","properties":[{"key":"EntryName","value":"EFG_V-like"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR005225","properties":[{"key":"EntryName","value":"Small_GTP-bd"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR035650","properties":[{"key":"EntryName","value":"Tet_C"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR005517","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2_IV"}]},{"database":"NCBIfam","id":"TIGR00231","properties":[{"key":"EntryName","value":"small_GTP"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF012153","properties":[{"key":"EntryName","value":"tet_protect"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033148","properties":[{"key":"EntryName","value":"tet_protect_M_W"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261:SF1","properties":[{"key":"EntryName","value":"RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261","properties":[{"key":"EntryName","value":"TRANSLATION ELONGATION FACTOR G-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00679","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14492","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03764","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"PRINTS","id":"PR01037","properties":[{"key":"EntryName","value":"TCRTETOQM"}]},{"database":"SUPFAM","id":"SSF54980","properties":[{"key":"EntryName","value":"EF-G C-terminal domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKIINLGILAHVDAGKTTLTESLLYTSGAIAELGSVDEGTTRTDTMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLAVLDGAILVISAKDGVQAQTRILFHALRKMNIPTVIFINKIDQAGVDLQSVVQSVRDKLSADIIIKQTVSLSPEIVLEENTDIEAWDAVIENNDALLEKYIAGEPISQEKLAREEQRRVQEASLFPVYHGSAKKGLGIQPLMDAVTGLFQPIGEQGSATLCGSVFKVEYTDCGQRRVYLRLYSGTLRLRDTVALAGREKLKITEMRIPSKGEIVRTDTAYPGEIVILPSDSVRLNDVLGDQTRLPRKRWREAPLPMLRTTIAPKTAAQRERLLDALTQLADTDPLLRCEVDSITHEIILSFLGRVQLEVVSALLSEKYKIETVVKEPTVIYMERPLKAASHTIHIEVPPNPFWASVGLSIEPLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYSPVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIVSTQIKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRPNSRIDKVRHMFNKLA","length":639,"molWeight":71433,"crc64":"4A7AF9852A3E6607","md5":"1DBD34AEE1F1692291E4D62FDFE945A8"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1},"uniParcId":"UPI0000006445"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q84FH1","uniProtkbId":"Q84FH1_MEGEL","entryAudit":{"firstPublicDate":"2003-06-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-06-01","entryVersion":107,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42740.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42740.1"}],"value":"Tetracycline resistance protein"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42740.1"}],"value":"tet"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"Tr-type G","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51722"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42740.1"}],"id":"KW-0195","category":"Molecular function","name":"Cyclin"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022917"}],"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"12839756","citationType":"journal article","authors":["Stanton T.B.","Humphrey S.B."],"citationCrossReferences":[{"database":"PubMed","id":"12839756"},{"database":"DOI","id":"10.1128/AEM.69.7.3874-3882.2003"}],"title":"Isolation of tetracycline-resistant Megasphaera elsdenii strains with novel mosaic gene combinations of tet(O) and tet(W) from swine.","publicationDate":"2003","journal":"Appl. Environ. Microbiol.","firstPage":"3874","lastPage":"3882","volume":"69"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42740.1"}],"value":"14-14","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO42740.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY196920","properties":[{"key":"ProteinId","value":"AAO42740.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_054336482.1","properties":[{"key":"NucleotideSequenceId","value":"NG_048285.1"}]},{"database":"AlphaFoldDB","id":"Q84FH1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q84FH1","properties":[{"key":"Description","value":"-"}]},{"database":"CARD","id":"ARO:3007123","properties":[{"key":"CARD short name","value":"tet(O/W/O)"},{"key":"Resistance mechanism identifier","value":"ARO:0001003"},{"key":"Resistance mechanism name","value":"antibiotic target protection"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd03711","properties":[{"key":"EntryName","value":"Tet_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03690","properties":[{"key":"EntryName","value":"Tet_II"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16258","properties":[{"key":"EntryName","value":"Tet_III"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01684","properties":[{"key":"EntryName","value":"Tet_like_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04168","properties":[{"key":"EntryName","value":"TetM_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.870","properties":[{"key":"EntryName","value":"Elongation Factor G (Translational Gtpase), domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR041095","properties":[{"key":"EntryName","value":"EFG_II"}]},{"database":"InterPro","id":"IPR035647","properties":[{"key":"EntryName","value":"EFG_III/V"}]},{"database":"InterPro","id":"IPR000640","properties":[{"key":"EntryName","value":"EFG_V-like"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR005225","properties":[{"key":"EntryName","value":"Small_GTP-bd"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR035650","properties":[{"key":"EntryName","value":"Tet_C"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR005517","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2_IV"}]},{"database":"NCBIfam","id":"TIGR00231","properties":[{"key":"EntryName","value":"small_GTP"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF012153","properties":[{"key":"EntryName","value":"tet_protect"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033148","properties":[{"key":"EntryName","value":"tet_protect_M_W"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261:SF1","properties":[{"key":"EntryName","value":"RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261","properties":[{"key":"EntryName","value":"TRANSLATION ELONGATION FACTOR G-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00679","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14492","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03764","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"PRINTS","id":"PR01037","properties":[{"key":"EntryName","value":"TCRTETOQM"}]},{"database":"SMART","id":"SM00889","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54980","properties":[{"key":"EntryName","value":"EF-G C-terminal domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKIINLGILAHVDAGKTTLTESLLYTSGAIAELGSVDEGTTRTDTMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLAVLDGAILVISAKDGVQAQTRILFHALRKMNIPTVIFINKIDQAGVDLQSVVQSVRDKLSADIIIKQTVSLSPEIVLEENTDIEAWDAVIENNDALLEKYIAGEPISQEKLAREEQRRVQEASLFPVYHGSAKKGLGIQPLMDAVTGLFQPIGEQGSATLCGSVFKVEYTDCGQRRVYLRLYSGTLRLRDTVALAGREKLKITEMRIPSKGEIVRTDTAYPGEIVILPSDSVRLNDVLGDQTRLPRKRWREAPLPMLRTTIAPKTAAQRERLLDALTQLADTDPLLRCEVDSITHEIILSFLGRVQLEVVSALLSEKYKIETVVKEPTVIYMERPLKAASHTIHIEVPPNPFWASIGLSVTPLPLGSGVQYESRVSLGYLNQSFQNAVRDGIRYGLEQGLFGWNVTDCKICFEYGLYYSPVSTPADFRSLAPIVLEQALKESGTQLLEPYLSFTLYAPQEYLSRAYHDAPRYCADIVSTQIKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRPNSRIDKVRHMFHKLA","length":639,"molWeight":71372,"crc64":"D734C230B10418E4","md5":"42624C3F6CD9760F55285666A899DBB8"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1},"uniParcId":"UPI0000006444"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q9RGN3","uniProtkbId":"Q9RGN3_MEGEL","entryAudit":{"firstPublicDate":"2000-05-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-05-01","entryVersion":125,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22114.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"4: Predicted","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22114.1"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AVO26160.1"}],"value":"Hydrogenase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22114.1"}],"value":"hydA"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AVO26160.1"}],"value":"C6Y28_00130"}]}],"features":[{"type":"Domain","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"4Fe-4S ferredoxin-type","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51379"}]},{"type":"Region","location":{"start":{"value":461,"modifier":"EXACT"},"end":{"value":484,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023014"}],"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"10669801","citationType":"journal article","authors":["Atta M.","Meyer J."],"citationCrossReferences":[{"database":"PubMed","id":"10669801"},{"database":"DOI","id":"10.1016/S0167-4838(99)00245-9"}],"title":"Characterization of the gene encoding the [Fe]-hydrogenase from Megasphaera elsdenii.","publicationDate":"2000","journal":"Biochim. Biophys. Acta","firstPage":"368","lastPage":"371","volume":"1476"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22114.1"}],"value":"ATCC25940","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22114.1"}]},{"referenceNumber":2,"citation":{"id":"CI-DK78A63ANU28R","citationType":"journal article","authors":["Hatmaker E.A.","O'Dell K.","Riley L.A.","Klingeman D.M.","Guss A.M."],"title":"Complete genomes of two Megasphaera elsdenii strains, NCIMB 702410 and ATCC 25940.","publicationDate":"2018","journal":"Genome Announc.","firstPage":"0","lastPage":"0","volume":"0"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AVO26160.1"}],"value":"NCIMB702410","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AVO26160.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF120457","properties":[{"key":"ProteinId","value":"AAF22114.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP027569","properties":[{"key":"ProteinId","value":"AVO26160.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_014016432.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_AP031433.1"}]},{"database":"AlphaFoldDB","id":"Q9RGN3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9RGN3","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"97490706","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9805142at2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0008901","properties":[{"key":"GoTerm","value":"F:ferredoxin hydrogenase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0051536","properties":[{"key":"GoTerm","value":"F:iron-sulfur cluster binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.30.70.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1780","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.950.10","properties":[{"key":"EntryName","value":"Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"4.10.260.20","properties":[{"key":"EntryName","value":"Iron hydrogenase, small subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR017896","properties":[{"key":"EntryName","value":"4Fe4S_Fe-S-bd"}]},{"database":"InterPro","id":"IPR017900","properties":[{"key":"EntryName","value":"4Fe4S_Fe_S_CS"}]},{"database":"InterPro","id":"IPR050340","properties":[{"key":"EntryName","value":"Cytosolic_Fe-S_CAF"}]},{"database":"InterPro","id":"IPR009016","properties":[{"key":"EntryName","value":"Fe_hydrogenase"}]},{"database":"InterPro","id":"IPR004108","properties":[{"key":"EntryName","value":"Fe_hydrogenase_lsu_C"}]},{"database":"InterPro","id":"IPR003149","properties":[{"key":"EntryName","value":"Fe_hydrogenase_ssu"}]},{"database":"InterPro","id":"IPR036991","properties":[{"key":"EntryName","value":"Fe_hydrogenase_ssu_sf"}]},{"database":"InterPro","id":"IPR013352","properties":[{"key":"EntryName","value":"Fe_hydrogenase_subset"}]},{"database":"NCBIfam","id":"TIGR02512","properties":[{"key":"EntryName","value":"FeFe_hydrog_A"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11615","properties":[{"key":"EntryName","value":"NITRATE, FORMATE, IRON DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02906","properties":[{"key":"EntryName","value":"Fe_hyd_lg_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02256","properties":[{"key":"EntryName","value":"Fe_hyd_SSU"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00037","properties":[{"key":"EntryName","value":"Fer4"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00902","properties":[{"key":"EntryName","value":"Fe_hyd_SSU"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54862","properties":[{"key":"EntryName","value":"4Fe-4S ferredoxins"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53920","properties":[{"key":"EntryName","value":"Fe-only hydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00198","properties":[{"key":"EntryName","value":"4FE4S_FER_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51379","properties":[{"key":"EntryName","value":"4FE4S_FER_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPEFHSRFEKIDRRVPIDEHNCAVQFDVTKCKNCTLCRRACADTQTVLDYYSLSSTGDMPICVHCGQCSSACPFGAIVEVNDVDKVKAALKDPEKIVIFQTAPAVRVGLGEAFGMDPGTFVEGKMVAALRTLGADYVFDTDFGADLTIMEEATELLHRLQSEEIPIPQFTSCCPAWVEFAETFYPDLLQHLSSTKSPISILSPVIKTYFAQQKNIDPKKIVNVCVTPCTAKKAEIRRPELSASGLFWDEPEIRDTDICITTRELAQWIQDENIDFASLEDSKFDKAFGEASGGGRIFGNSGGVMEAAIRTAYHMFTGRPAPKDFIPFEPVRGLQGVKKATVIFGHFVLHVAAISGLGNARAFIDDLIKNDAFEDYSFIEVMACPGGCIGGGGQPKVKLPQVKKVQEARTASIYKSDEETDIKASWQNPEIETLYEAFLDEPLSEMAEFTLHTYFSDKSDQLGRMKNLTPQTNPMSPKYKPPTEE","length":484,"molWeight":53601,"crc64":"0596BEE31FDBCEE9","md5":"652CDDC1F88CFC90DB307D7B7F1BD5C9"},"extraAttributes":{"countByFeatureType":{"Domain":1,"Region":1},"uniParcId":"UPI00000BE6B3"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q9RGN4","uniProtkbId":"Q9RGN4_MEGEL","entryAudit":{"firstPublicDate":"2000-05-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-05-01","entryVersion":66,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Megasphaera elsdenii","taxonId":907,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22113.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Negativicutes","Veillonellales","Veillonellaceae","Megasphaera"]},"proteinExistence":"4: Predicted","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22113.1"}],"value":"Putative ABC transporter"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22113.1"}],"value":"abc"}}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"ABC transporter","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00005"}]},{"type":"Domain","location":{"start":{"value":203,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"ABC transporter Uup C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF16326"}]},{"type":"Coiled coil","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":262,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Coils"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22113.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Coils"}],"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"10669801","citationType":"journal article","authors":["Atta M.","Meyer J."],"citationCrossReferences":[{"database":"PubMed","id":"10669801"},{"database":"DOI","id":"10.1016/S0167-4838(99)00245-9"}],"title":"Characterization of the gene encoding the [Fe]-hydrogenase from Megasphaera elsdenii.","publicationDate":"2000","journal":"Biochim. Biophys. Acta","firstPage":"368","lastPage":"371","volume":"1476"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22113.1"}],"value":"ATCC25940","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAF22113.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF120457","properties":[{"key":"ProteinId","value":"AAF22113.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q9RGN4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016887","properties":[{"key":"GoTerm","value":"F:ATP hydrolysis activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.287.380","properties":[{"key":"EntryName","value":"Valyl-tRNA synthetase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR032524","properties":[{"key":"EntryName","value":"ABC_tran_C"}]},{"database":"InterPro","id":"IPR003439","properties":[{"key":"EntryName","value":"ABC_transporter-like_ATP-bd"}]},{"database":"InterPro","id":"IPR051309","properties":[{"key":"EntryName","value":"ABCF_ATPase"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR037118","properties":[{"key":"EntryName","value":"Val-tRNA_synth_C_sf"}]},{"database":"PANTHER","id":"PTHR42855","properties":[{"key":"EntryName","value":"ABC TRANSPORTER ATP-BINDING SUBUNIT"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42855:SF1","properties":[{"key":"EntryName","value":"ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00005","properties":[{"key":"EntryName","value":"ABC_tran"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16326","properties":[{"key":"EntryName","value":"ABC_tran_CTD"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"STLLKVLAGQLAPTAGTVTIGQTVRFGFFTQQLPQFDEGMRVIDYVQEHGHFVTNQFGQRISATRLLDQFLFTEEMQYTYIAKLSGGERRRLYLLRLLMDQPNVLLLDEPTNDLDIPTLTVLERYLDTFQGVVLVVSHDRYFLDRVVDKLFTLEKGTWDRFYGDYSEYLEEKYSHHQEKKGEKATAPSITKTNITPAKTKAGLTSRQEEELRRITEELPRYEAMLKGLNAAIAAAGSNYEEVESLLSEQKETQEKVDALTERWCELEDLKA","length":271,"molWeight":31144,"crc64":"FB51863B4352CD7C","md5":"CBD1403BDFB8683779F365472462E1A8"},"extraAttributes":{"countByFeatureType":{"Domain":2,"Coiled coil":1,"Non-terminal residue":1},"uniParcId":"UPI00000B5566"}}]}