{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P81702","secondaryAccessions":["Q8NJ53"],"uniProtkbId":"CEPL_CERFI","entryAudit":{"firstPublicDate":"1999-07-15","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2024-10-02","entryVersion":81,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Ceratocystis fimbriata f. sp. platani","taxonId":88771,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Sordariomycetes","Hypocreomycetidae","Microascales","Ceratocystidaceae","Ceratocystis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10455173"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15063505"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33736287"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"CAC84090.2"}],"value":"Cerato-platanin"}},"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15063505"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16679539"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AGQ22226.1"}],"value":"cp"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"KKF93197.1"}],"value":"CFO_g4460"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10455173"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16679539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21454637"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23512479"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24968226"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33736287"}],"value":"Phytotoxin which causes production of phytoalexin in platanus acerifolia, platanus occidentalis and platanus orientalis (PubMed:10455173, PubMed:16679539, PubMed:21454637, PubMed:24968226, PubMed:33736287). Induces cell necrosis in tobacco leaves, and in callus cells and leaves of P.acerifolia (PubMed:10455173). Induces reactive oxygen species (ROS) synthesis, nitric oxide (NO) production and mitogen-activated protein kinases (MAPKs) phosphorylation in the leaves of A.thaliana and P.acerifolia (PubMed:33736287). Results in H(2)O(2) production on the epidermis around the stomata, rapid closure of the stomata, reduced photosynthetic and CO(2) assimilation rate, and an increase in a number of volatile organic compound (VOC) emission in A.thaliana leaves (PubMed:24968226). Induces overexpression of genes related to salicylic acid- and ethylene-signaling, camalexin synthesis, ROS production and oxidative stress, and genes of various receptor kinases, and down-regulation of a number of jasmonic acid (JA)-signaling genes in A.thaliana leaves (PubMed:24968226). Renders resistance against C.platani in A.thaliana and P.acerifolia (PubMed:24968226). Renders localized resistance of A.thaliana against infection by virulent foliar pathogens B.cinerea and P.syringae pv. tomato (PubMed:24968226). Binds cellulose analog carboxymethyl cellulose (CMC) (PubMed:33736287). Expansin-like protein with probable fungal and plant cell wall loosening activity based on its non-enzymatic cellulose weakening activity in vitro (PubMed:23512479, PubMed:33736287). Increases glucose production by cellulase after pre-incubation of cellulose with this protein (PubMed:33736287). In contrast, according to PubMed:23512479, no synergistic effect with cellulases (PubMed:23512479). May have a structural role in the fungal cell wall based on its ability to bind chitin, but does not bind beta-1,3-glucan (PubMed:23512479)"}],"commentType":"FUNCTION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23512479"}],"value":"Optimum pH is 5.0 for the non-enzymatic cellulose weakening activity."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23512479"}],"value":"Optimum temperature is 38 degrees Celsius for the non-enzymatic cellulose weakening activity."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16679539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21454637"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33736287"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15063505"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19387635"}],"value":"Localizes on the cell walls of hyphae and conidia (PubMed:15063505, PubMed:19387635). Localizes on the cell walls of ascospores (PubMed:15063505). More abundant on the conidial and ascospore walls than on the hyphal wall (PubMed:15063505). Distributes across the entire thickness of the conidial wall (PubMed:15063505). More densely localized on the layer near the plasma membrane than on the more outward layer of the ascospore wall (PubMed:15063505). Also localizes on the fibrillar material at the surface of ascospores (PubMed:15063505)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10455173"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15063505"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16679539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19387635"}],"value":"Secreted","id":"SL-0243"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15063505"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19387635"}],"value":"Secreted, cell wall","id":"SL-0041"}}]},{"commentType":"MASS SPECTROMETRY","method":"MALDI","molWeight":12383.6,"molWeightError":0.0,"note":"The measured mass is that of the mature protein.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10455173"}]},{"commentType":"MASS SPECTROMETRY","method":"MALDI","molWeight":12395.0,"molWeightError":0.0,"note":"The measured mass is that of the mature protein.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16679539"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"33736287"}],"value":"Generated Asn-98 mutant protein displays improved cellulose weakening activity, thus making it a potential candidate in the production of biofuel as an enhancer of enzymatic hydrolysis of cellulosic materials"}],"commentType":"BIOTECHNOLOGY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the cerato-platanin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":14,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10455173"}]},{"type":"Chain","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"Cerato-platanin","evidences":[{"evidenceCode":"ECO:0000255"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10455173"}],"featureId":"PRO_0000156113"},{"type":"Region","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"Binding of oligomers of N-acetylglucosamine (GlcNAc)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21454637"}]},{"type":"Region","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"Binding of N-acetylglucosamine tetramer (GlcNAc-4)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21454637"}]},{"type":"Region","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"Binding of N-acetylglucosamine tetramer (GlcNAc-4)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21454637"}]},{"type":"Region","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"Binding of N-acetylglucosamine tetramer (GlcNAc-4)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21454637"}]},{"type":"Region","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"Binding of oligomers of N-acetylglucosamine (GlcNAc)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21454637"}]},{"type":"Disulfide bond","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10455173"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16679539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21454637"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2KQA"}]},{"type":"Disulfide bond","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10455173"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16679539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21454637"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2KQA"}]},{"type":"Mutagenesis","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"Altered protein stability due to dramatic change in structure without altering the net charge of the protein. 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