{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0DXK6","uniProtkbId":"OGAS_PHLCY","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2026-06-10","entryVersion":3,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Phlegmariurus cryptomerinus","commonName":"Tropical firmoss","synonyms":["Huperzia cryptomerina"],"taxonId":872610,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Lycopodiopsida","Lycopodiales","Lycopodiaceae","Huperzioideae","Phlegmariurus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A9E9KUU8"}],"value":"3-oxoglutarate synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A9E9KUU8"}],"value":"PcOGAS"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33104367"}],"value":"2.3.1.333"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33104367"}],"value":"Pyrrolidine ketide synthase 1"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33104367"}],"value":"PcPKS1"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A9E9KUU8"}],"value":"OGAS"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33104367"}],"value":"PKS1"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33104367"}],"value":"Non-canonical type III polyketide synthase involved in the biosynthesis of quinolizidine (e.g. huperzines A and B, lycodine and lycopodine) and piperidine (e.g. pelletierine) natural products, acting as toxic repellents against herbivorous predators and used as memory and concentration enhancer, being inhibitors of the human acetylcholinesterase; pelletierine is a powerful vermifuge, particularly against tapeworms (PubMed:33104367). Catalyzes the formation of 3-oxoglutarate from malonyl-CoA, which subsequently undergoes a non-enzymatic Mannich-like condensation with delta1-piperideine to produce 4-(2-piperidyl)acetoacetate (4PAA) and 4PAA-CoA, precursors of various phlegmarine-derived and pelletierine-derived alkaloids in Lycopodiaceae (PubMed:33104367)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 malonyl-CoA + H2O = 3-oxoglutarate + CO2 + 2 CoA","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:85355"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57384"},{"database":"ChEBI","id":"CHEBI:234323"}],"ecNumber":"2.3.1.333","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33104367"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:85356"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33104367"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33104367"}],"value":"Alkaloid biosynthesis; tropane alkaloid biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0AAV8SIN5"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":399,"modifier":"EXACT"}},"description":"3-oxoglutarate synthase","featureId":"PRO_0000464213"},{"type":"Active site","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"Nucleophile; acyl-thioester intermediate","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10023"}]},{"type":"Active site","location":{"start":{"value":312,"modifier":"EXACT"},"end":{"value":312,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}]},{"type":"Binding site","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57384"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"malonyl-CoA","id":"ChEBI:CHEBI:57384"}},{"type":"Binding site","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57384"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"malonyl-CoA","id":"ChEBI:CHEBI:57384"}},{"type":"Binding site","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:234323"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"3-oxoglutarate","id":"ChEBI:CHEBI:234323"}},{"type":"Binding site","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57384"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"malonyl-CoA","id":"ChEBI:CHEBI:57384"}},{"type":"Binding site","location":{"start":{"value":312,"modifier":"EXACT"},"end":{"value":312,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:234323"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"3-oxoglutarate","id":"ChEBI:CHEBI:234323"}},{"type":"Binding site","location":{"start":{"value":312,"modifier":"EXACT"},"end":{"value":312,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57384"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"malonyl-CoA","id":"ChEBI:CHEBI:57384"}},{"type":"Binding site","location":{"start":{"value":345,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:234323"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"3-oxoglutarate","id":"ChEBI:CHEBI:234323"}},{"type":"Binding site","location":{"start":{"value":345,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57384"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"malonyl-CoA","id":"ChEBI:CHEBI:57384"}},{"type":"Binding site","location":{"start":{"value":347,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:234323"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"3-oxoglutarate","id":"ChEBI:CHEBI:234323"}},{"type":"Binding site","location":{"start":{"value":347,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57384"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A5J6BTZ3"}],"ligand":{"name":"malonyl-CoA","id":"ChEBI:CHEBI:57384"}}],"keywords":[{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"33104367","citationType":"journal article","authors":["Wang J.","Zhang Z.-K.","Jiang F.-F.","Qi B.-W.","Ding N.","Hnin S.Y.Y.","Liu X.","Li J.","Wang X.-H.","Tu P.-F.","Abe I.","Morita H.","Shi S.-P."],"citationCrossReferences":[{"database":"PubMed","id":"33104367"},{"database":"DOI","id":"10.1021/acs.orglett.0c03339"}],"title":"Deciphering the biosynthetic mechanism of pelletierine in Lycopodium alkaloid biosynthesis.","publicationDate":"2020","journal":"Org. Lett.","firstPage":"8725","lastPage":"8729","volume":"22"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","FUNCTION","CATALYTIC ACTIVITY","PATHWAY"]}],"uniProtKBCrossReferences":[{"database":"UniPathway","id":"UPA00330","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0016747","properties":[{"key":"GoTerm","value":"F:acyltransferase activity, transferring groups other than amino-acyl groups"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0030639","properties":[{"key":"GoTerm","value":"P:polyketide biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd00831","properties":[{"key":"EntryName","value":"CHS_like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.47.10:FF:000014","properties":[{"key":"EntryName","value":"Chalcone synthase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.47.10:FF:000025","properties":[{"key":"EntryName","value":"Chalcone synthase 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.47.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR001099","properties":[{"key":"EntryName","value":"Chalcone/stilbene-like_synt_N"}]},{"database":"InterPro","id":"IPR012328","properties":[{"key":"EntryName","value":"Chalcone/stilbene_synt_C"}]},{"database":"InterPro","id":"IPR011141","properties":[{"key":"EntryName","value":"Polyketide_synthase_type-III"}]},{"database":"InterPro","id":"IPR016039","properties":[{"key":"EntryName","value":"Thiolase-like"}]},{"database":"PANTHER","id":"PTHR11877","properties":[{"key":"EntryName","value":"HYDROXYMETHYLGLUTARYL-COA SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02797","properties":[{"key":"EntryName","value":"Chal_sti_synt_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00195","properties":[{"key":"EntryName","value":"Chal_sti_synt_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000451","properties":[{"key":"EntryName","value":"PKS_III"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53901","properties":[{"key":"EntryName","value":"Thiolase-like"},{"key":"MatchStatus","value":"2"}]}],"sequence":{"value":"MATATRPSERCEGGLATVLAIGTANPPNSIDQSAYPDFFFKATNCEAQTELKTKFQRICDRSGIKKRHFVLTEDLLKENPSICSYRQPSLEARQDIAIQAIPKLAHEAAAKAIEEWGQPKSSITHVVFATRSGASMPGADLILANLLGLKSSVKRCMLYHQGCFAGGSAMRIAKDMAENNKGARVLVACSETNVATFRAPSESHPDGLVVAALFGDGAAAVIVGSDPIAESTEKPLFEIHWASEDVISKSDGAVVGHLSEAGLIYHLHKDVPGLISKNIESILKEAVSISNCTTSGEDAVDIDWNDMFWAVHPGGRAILDQVERQLKLKPSKFEATRDVLATYGNMSSACVLFVLDQIRKRSQELKLSTSGEGCKWGFLLAFGPGLTVETLVLKSIRQY","length":399,"molWeight":43121,"crc64":"4FB29B5B517CBDCD","md5":"99E38E03AA16720B2EEE1F8B75158086"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":2,"Binding site":10},"uniParcId":"UPI0046EDDB2A"}}]}