{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q53047","secondaryAccessions":["P14914"],"uniProtkbId":"OMPB_RICRI","entryAudit":{"firstPublicDate":"2000-05-30","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1996-11-01","entryVersion":102,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Outer membrane protein B"}},"alternativeNames":[{"fullName":{"value":"168 kDa surface-layer protein"}},{"fullName":{"value":"Cell surface antigen 5"},"shortNames":[{"value":"Sca5"}]},{"fullName":{"value":"Surface protein antigen"}},{"fullName":{"value":"rOmp B"},"shortNames":[{"value":"rOmpB"}]}],"contains":[{"recommendedName":{"fullName":{"value":"120 kDa surface-exposed protein"}},"alternativeNames":[{"fullName":{"value":"120 kDa outer membrane protein OmpB"}},{"fullName":{"value":"Surface protein antigen"}},{"fullName":{"value":"p120"}}]},{"recommendedName":{"fullName":{"value":"32 kDa beta peptide"}}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"ompB"}}],"comments":[{"texts":[{"value":"The 120 kDa surface-exposed protein is a major structural protein which may play a role as a rickettsial virulence factor and/or immunogen during infection"}],"commentType":"FUNCTION"},{"texts":[{"value":"The 32 kDa beta peptide may serve as a membrane anchor"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","molecule":"Outer membrane protein B","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Periplasm","id":"SL-0200"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"120 kDa surface-exposed protein","note":{"texts":[{"value":"Surface exposed. This bacterium is covered by a S-layer with hexagonal symmetry"}]},"subcellularLocations":[{"location":{"value":"Secreted","id":"SL-0243"}},{"location":{"value":"Cell surface","id":"SL-0310"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"32 kDa beta peptide","note":{"texts":[{"value":"The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell outer membrane","id":"SL-0040"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"value":"The N-terminus is blocked"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the rickettsiae OmpA/OmpB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1654,"modifier":"EXACT"}},"description":"Outer membrane protein B","featureId":"PRO_0000387582"},{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1333,"modifier":"EXACT"}},"description":"120 kDa surface-exposed protein","featureId":"PRO_0000032657"},{"type":"Propeptide","location":{"start":{"value":1334,"modifier":"EXACT"},"end":{"value":1361,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000032658"},{"type":"Chain","location":{"start":{"value":1362,"modifier":"EXACT"},"end":{"value":1654,"modifier":"EXACT"}},"description":"32 kDa beta peptide","featureId":"PRO_0000032659"},{"type":"Domain","location":{"start":{"value":1366,"modifier":"EXACT"},"end":{"value":1654,"modifier":"EXACT"}},"description":"Autotransporter","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00556"}]}],"keywords":[{"id":"KW-0998","category":"Cellular component","name":"Cell outer membrane"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0574","category":"Cellular component","name":"Periplasm"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1134","category":"Domain","name":"Transmembrane beta strand"},{"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"1724278","citationType":"journal article","authors":["Gilmore R.D. Jr.","Cieplak W. Jr.","Policastro P.F.","Hackstadt T."],"citationCrossReferences":[{"database":"PubMed","id":"1724278"},{"database":"DOI","id":"10.1111/j.1365-2958.1991.tb02082.x"}],"title":"The 120 kilodalton outer membrane protein (rOmp B) of Rickettsia rickettsii is encoded by an unusually long open reading frame: evidence for protein processing from a large precursor.","publicationDate":"1991","journal":"Mol. Microbiol.","firstPage":"2361","lastPage":"2370","volume":"5"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","PARTIAL PROTEIN SEQUENCE (N-TERMINAL OF THE 32 KDA BETA PEPTIDE)"],"referenceComments":[{"value":"R","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"2515418","citationType":"journal article","authors":["Gilmore R.D. Jr.","Joste N.","McDonald G.A."],"citationCrossReferences":[{"database":"PubMed","id":"2515418"},{"database":"DOI","id":"10.1111/j.1365-2958.1989.tb00143.x"}],"title":"Cloning, expression and sequence analysis of the gene encoding the 120 kD surface-exposed protein of Rickettsia rickettsii.","publicationDate":"1989","journal":"Mol. Microbiol.","firstPage":"1579","lastPage":"1586","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-1654"],"referenceComments":[{"value":"R","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"1809847","citationType":"journal article","authors":["Gilmore R.D. Jr.","Joste N.","McDonald G.A."],"citationCrossReferences":[{"database":"PubMed","id":"1809847"}],"publicationDate":"1991","journal":"Mol. Microbiol.","firstPage":"3089","lastPage":"3089","volume":"5"},"referencePositions":["ERRATUM OF PUBMED:2515418","SEQUENCE REVISION TO 1348-1358"]},{"referenceNumber":4,"citation":{"id":"1729180","citationType":"journal article","authors":["Hackstadt T.","Messer R.","Cieplak W. Jr.","Peacock M.G."],"citationCrossReferences":[{"database":"PubMed","id":"1729180"},{"database":"DOI","id":"10.1128/iai.60.1.159-165.1992"}],"title":"Evidence for proteolytic cleavage of the 120-kilodalton outer membrane protein of rickettsiae: identification of an avirulent mutant deficient in processing.","publicationDate":"1992","journal":"Infect. Immun.","firstPage":"159","lastPage":"165","volume":"60"},"referencePositions":["PROTEIN SEQUENCE OF 1362-1380","CLEAVAGE SITE"],"referenceComments":[{"value":"R","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X16353","properties":[{"key":"ProteinId","value":"CAA34403.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X16353","properties":[{"key":"ProteinId","value":"CAA34402.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S07575","properties":[{"key":"EntryName","value":"S07575"}]},{"database":"PIR","id":"S18227","properties":[{"key":"EntryName","value":"S18227"}]},{"database":"RefSeq","id":"WP_012151219.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP098689.1"}]},{"database":"AlphaFoldDB","id":"Q53047","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q53047","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937732","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009279","properties":[{"key":"GoTerm","value":"C:cell outer membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0009986","properties":[{"key":"GoTerm","value":"C:cell surface"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0042597","properties":[{"key":"GoTerm","value":"C:periplasmic space"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"Gene3D","id":"2.40.128.130","properties":[{"key":"EntryName","value":"Autotransporter beta-domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005546","properties":[{"key":"EntryName","value":"Autotransporte_beta"}]},{"database":"InterPro","id":"IPR036709","properties":[{"key":"EntryName","value":"Autotransporte_beta_dom_sf"}]},{"database":"InterPro","id":"IPR006315","properties":[{"key":"EntryName","value":"OM_autotransptr_brl_dom"}]},{"database":"InterPro","id":"IPR022095","properties":[{"key":"EntryName","value":"OmpB_passenger_Rickettsia"}]},{"database":"InterPro","id":"IPR048195","properties":[{"key":"EntryName","value":"OmpB_ricketsia"}]},{"database":"NCBIfam","id":"TIGR01414","properties":[{"key":"EntryName","value":"autotrans_barl"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF041657","properties":[{"key":"EntryName","value":"ompB_ricketsia"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03797","properties":[{"key":"EntryName","value":"Autotransporter"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12334","properties":[{"key":"EntryName","value":"rOmpB_passenger"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00869","properties":[{"key":"EntryName","value":"Autotransporter"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103515","properties":[{"key":"EntryName","value":"Autotransporter"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51208","properties":[{"key":"EntryName","value":"AUTOTRANSPORTER"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAQKPNFLKKLISAGLVTASTATIVASFAGSAMGAAIQQNRTTNGAATTVDGAGFDQTAAPANVGVALNAVITANANNGINFNTPAGSFNGLLLNTANNLAVTVSEDTTLGFITNVVHNAHSFNLTLNAGKTLTITGQGVTNAQAAATKNAQNVVVQFNNGAAIDNNDLKGVGRIDFGAPASTLVFNLANPTTQKAPLILGDNAVIANGVNGTLNVTNGFIQVSNKSFATVKAINIADGQGIIFNTDANNANTLNLQAGGTTINFTGTDGTGRLVLLSKHAAATNFNITGSLGGNLKGVIEFNTVAVDGQLTANAGAANAVIGTNNGAGRAAGFVVSVDNGKVATIDGQVYAKDMVIQSANATGQVNFRHIVDVGADGTTAFKTAASKVTITQDSNFGNTDFGNLAAQIKVPNAITLTGNFTGDASNPGNTAGVITFDANGTLESASADANVAVTNNITAIEASGAGVVQLSGTHAAELRLGNAGSIFKLADGTVINGKVNQTALVGGALAAGTITLDGSATITGDIGNAGGAAALQRITLANDAKKTLTLGGANIIGAGGGTIDLQANGGTIKLTSTQNNIVVDFDLAIATDQTGVVDASSLTNAQTLTINGKIGTIGANNKTLGQFNIGSSKTVLSNGNVAINELVIGNDGAVQFAHDTYLITRTTNAAGQGKIIFNPVVNNGTTLAAGTNLGSATNPLAEINFGSKGVNVDTVLNVGEGVNLYATNITTTDANVGSFVFNAGGTNIVSGTVGGQQGNKFNTVALENGTTVKFLGNATFNGNTTIAANSTLQIGGNYTADCVASADGTGIVEFVNTGPITVTLNKQAAPVNALKQITVSGPGNVVINEIGNAGNHHGAVTDTIAFENSSLGAVVFLPRGIPFNDAGNTMPLTIKSTVGNKTAKGFDVPSVVVLGVDSVIADGQVIGDQNNIVGLGLGSDNGIIVNATTLYAGISTLNNNQGTVTLSGGVPNTPGTVYGLGTGIGASKFKQVTFTTDYNNLGNIIATNATINDGVTVTTGGIAGIGFDGKITLGSVNGNGNVRFADGILSNSTSMIGTTKANNGTVTYLGNAFVGNIGDSDTPVASVRFTGSDSGAGLQGNIYSQVIDFGTYNLGIVNSNIILGGGTTAINGKIDLVTNTLTFASGTSTWGNNTSIETTLTLANGNIGHIVILEGAQVNTTTTGTTTIKVQDNANANFSGTQTYTLIQGGARFNGTLGSPNFAVTGSNRFVNYSLIRAANQDYVITRTNNAENVVTNDIANSPFGGAPGVDQNVTTFVNATNTAAYNNLLLAKNSANSANFVGAIVTDTSAAITNVQLDLAKDIQAQLGNRLGALRYLGTPETAEMAGPEAGAISAAVAAGDEAIDNVAYGIWAKPFYTDAHQSKKGGLAGYKAKTTGVVIGLDTLANDNLMIGAAIGITKTDIKHQDYKKGDKTDVNGFSFSLYGAQQLVKNFFAQGSAIFSLNQVKNKSQRYFFDANGNMSKQIAAGHYDNMTFGGNLTVGYDYNAMQGVLVTPMAGLSYLKSSDENYKETGTTVANKQVNSKFSDRTDLIVGAKVAGSTMNITDLAVYPEVHAFVVHKVTGRLSKTQSVLDGQVTPCINQPDRTTKTSYNLGLSASIRSDAKMEYGIGYDAQISSKYTAHQGTLKVRVNF","length":1654,"molWeight":168184,"crc64":"D7AB70FB7087F618","md5":"0A7EA4A00F259AA17EBA759B6667EC26"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"SUBCELLULAR LOCATION":3,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Chain":3,"Propeptide":1,"Domain":1},"uniParcId":"UPI0000130D00"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9AKJ9","uniProtkbId":"DAPA_RICRI","entryAudit":{"firstPublicDate":"2001-06-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":98,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"value":"4-hydroxy-tetrahydrodipicolinate synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"value":"HTPA synthase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"value":"4.3.3.7"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"value":"dapA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"value":"Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:34171"},{"database":"ChEBI","id":"CHEBI:15361"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:67139"},{"database":"ChEBI","id":"CHEBI:537519"}],"ecNumber":"4.3.3.7","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"value":"Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"value":"Homotetramer; dimer of dimers"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"value":"Belongs to the DapA family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"4-hydroxy-tetrahydrodipicolinate synthase","featureId":"PRO_0000103147"},{"type":"Active site","location":{"start":{"value":135,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"Proton donor/acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}]},{"type":"Active site","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"Schiff-base intermediate with substrate","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}]},{"type":"Binding site","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15361"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"ligand":{"name":"pyruvate","id":"ChEBI:CHEBI:15361"}},{"type":"Binding site","location":{"start":{"value":205,"modifier":"EXACT"},"end":{"value":205,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15361"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}],"ligand":{"name":"pyruvate","id":"ChEBI:CHEBI:15361"}},{"type":"Site","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"Part of a proton relay during catalysis","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}]},{"type":"Site","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"Part of a proton relay during catalysis","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00418"}]}],"keywords":[{"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0220","category":"Biological process","name":"Diaminopimelate biosynthesis"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0457","category":"Biological process","name":"Lysine biosynthesis"},{"id":"KW-0704","category":"Ligand","name":"Schiff base"}],"references":[{"referenceNumber":1,"citation":{"id":"11319266","citationType":"journal article","authors":["Andersson J.O.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"11319266"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a003864"}],"title":"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes.","publicationDate":"2001","journal":"Mol. Biol. Evol.","firstPage":"829","lastPage":"839","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"84-21C","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ293311","properties":[{"key":"ProteinId","value":"CAC33657.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012150786.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"Q9AKJ9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9AKJ9","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937339","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"GMDACVP","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00034","properties":[{"key":"RectionId","value":"UER00017"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008700","properties":[{"key":"GoTerm","value":"F:(R,S)-4-hydroxy-2-oxoglutarate aldolase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0008840","properties":[{"key":"GoTerm","value":"F:4-hydroxy-tetrahydrodipicolinate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009436","properties":[{"key":"GoTerm","value":"P:glyoxylate catabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd00950","properties":[{"key":"EntryName","value":"DHDPS"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00418","properties":[{"key":"EntryName","value":"DapA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR005263","properties":[{"key":"EntryName","value":"DapA"}]},{"database":"InterPro","id":"IPR002220","properties":[{"key":"EntryName","value":"DapA-like"}]},{"database":"InterPro","id":"IPR020625","properties":[{"key":"EntryName","value":"Schiff_base-form_aldolases_AS"}]},{"database":"InterPro","id":"IPR020624","properties":[{"key":"EntryName","value":"Schiff_base-form_aldolases_CS"}]},{"database":"NCBIfam","id":"TIGR00674","properties":[{"key":"EntryName","value":"dapA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12128:SF66","properties":[{"key":"EntryName","value":"4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12128","properties":[{"key":"EntryName","value":"DIHYDRODIPICOLINATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00701","properties":[{"key":"EntryName","value":"DHDPS"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001365","properties":[{"key":"EntryName","value":"DHDPS"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00146","properties":[{"key":"EntryName","value":"DHPICSNTHASE"}]},{"database":"SMART","id":"SM01130","properties":[{"key":"EntryName","value":"DHDPS"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00665","properties":[{"key":"EntryName","value":"DHDPS_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00666","properties":[{"key":"EntryName","value":"DHDPS_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MHNIFKGLITALITPFKDNKLDLYALERIVKHQIKHEVDAILIAGSTGESSSLSFEEYKLLLQTSVEIVNKCIPIISGCSSNNTTYARALAAESTKIGVDGFMASPPSYVKPTQHGIYKHFEALHEACNLPIMLYSAPTRSGVDFSDETILRLSKLPRILALKDCGVDLERPLRIRATVKKDFNILTGNDEVVLAFNAQGGVGWTSVASNIVPNICKELLEKWNKNDTKGALEIHQKLLPLYTALFVESNPIPIKYAAHYLGLCENEIRPPLTEASDSAKKQIENIITSLSIKI","length":294,"molWeight":32490,"crc64":"F8198780E49C73C3","md5":"67F5B97310C9392C6706888316484D4E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":1,"Active site":2,"Binding site":2,"Site":2},"uniParcId":"UPI0000128E5E"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P15921","uniProtkbId":"OMPA_RICRI","entryAudit":{"firstPublicDate":"1990-04-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1990-04-01","entryVersion":101,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Outer membrane protein A"}},"alternativeNames":[{"fullName":{"value":"190 kDa antigen"}},{"fullName":{"value":"Cell surface antigen"}},{"fullName":{"value":"rOmp A"},"shortNames":[{"value":"rOmpA"}]}],"contains":[{"recommendedName":{"fullName":{"value":"120 kDa surface-exposed protein"}},"alternativeNames":[{"fullName":{"value":"120 kDa outer membrane protein OmpA"}}]},{"recommendedName":{"fullName":{"value":"32 kDa beta peptide"}}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"ompA"}}],"comments":[{"texts":[{"value":"Elicits protective immunity"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","molecule":"Outer membrane protein A","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Periplasm","id":"SL-0200"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"120 kDa surface-exposed protein","note":{"texts":[{"value":"Surface exposed. This bacterium is covered by a S-layer with hexagonal symmetry"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Secreted","id":"SL-0243"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cell surface","id":"SL-0310"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"32 kDa beta peptide","note":{"texts":[{"value":"The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell outer membrane","id":"SL-0040"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Glycosylated"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the rickettsiae OmpA/OmpB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":2249,"modifier":"EXACT"}},"description":"Outer membrane protein A","featureId":"PRO_0000032647"},{"type":"Chain","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":null,"modifier":"UNKNOWN"}},"description":"120 kDa surface-exposed protein","featureId":"PRO_0000387577"},{"type":"Chain","location":{"start":{"value":null,"modifier":"UNKNOWN"},"end":{"value":2249,"modifier":"EXACT"}},"description":"32 kDa beta peptide","featureId":"PRO_0000387578"},{"type":"Repeat","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"Type I 1"},{"type":"Repeat","location":{"start":{"value":287,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"Type II 1"},{"type":"Repeat","location":{"start":{"value":359,"modifier":"EXACT"},"end":{"value":430,"modifier":"EXACT"}},"description":"Type II 2"},{"type":"Repeat","location":{"start":{"value":431,"modifier":"EXACT"},"end":{"value":505,"modifier":"EXACT"}},"description":"Type I 2"},{"type":"Repeat","location":{"start":{"value":506,"modifier":"EXACT"},"end":{"value":577,"modifier":"EXACT"}},"description":"Type II 3"},{"type":"Repeat","location":{"start":{"value":578,"modifier":"EXACT"},"end":{"value":652,"modifier":"EXACT"}},"description":"Type I 3"},{"type":"Repeat","location":{"start":{"value":653,"modifier":"EXACT"},"end":{"value":724,"modifier":"EXACT"}},"description":"Type II 4"},{"type":"Repeat","location":{"start":{"value":725,"modifier":"EXACT"},"end":{"value":799,"modifier":"EXACT"}},"description":"Type I 4"},{"type":"Repeat","location":{"start":{"value":800,"modifier":"EXACT"},"end":{"value":874,"modifier":"EXACT"}},"description":"Type I 5"},{"type":"Repeat","location":{"start":{"value":875,"modifier":"EXACT"},"end":{"value":949,"modifier":"EXACT"}},"description":"Type I 6"},{"type":"Repeat","location":{"start":{"value":950,"modifier":"EXACT"},"end":{"value":1021,"modifier":"EXACT"}},"description":"Type II 5"},{"type":"Repeat","location":{"start":{"value":1022,"modifier":"EXACT"},"end":{"value":1093,"modifier":"EXACT"}},"description":"Type II 6"},{"type":"Repeat","location":{"start":{"value":1094,"modifier":"EXACT"},"end":{"value":1165,"modifier":"EXACT"}},"description":"Type II 7"},{"type":"Repeat","location":{"start":{"value":1166,"modifier":"EXACT"},"end":{"value":1180,"modifier":"EXACT"}},"description":"Type I 7; truncated"},{"type":"Domain","location":{"start":{"value":1962,"modifier":"EXACT"},"end":{"value":2249,"modifier":"EXACT"}},"description":"Autotransporter","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00556"}]},{"type":"Region","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":1180,"modifier":"EXACT"}},"description":"14 X approximate tandem repeats"}],"keywords":[{"id":"KW-0998","category":"Cellular component","name":"Cell outer membrane"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0574","category":"Cellular component","name":"Periplasm"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1134","category":"Domain","name":"Transmembrane beta strand"}],"references":[{"referenceNumber":1,"citation":{"id":"2117568","citationType":"journal article","authors":["Anderson B.E.","McDonald G.A.","Jones D.C.","Regnery R.L."],"citationCrossReferences":[{"database":"PubMed","id":"2117568"},{"database":"DOI","id":"10.1128/iai.58.9.2760-2769.1990"}],"title":"A protective protein antigen of Rickettsia rickettsii has tandemly repeated, near-identical sequences.","publicationDate":"1990","journal":"Infect. Immun.","firstPage":"2760","lastPage":"2769","volume":"58"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"R","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M31227","properties":[{"key":"ProteinId","value":"AAA26380.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A41477","properties":[{"key":"EntryName","value":"A41477"}]},{"database":"AlphaFoldDB","id":"P15921","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009279","properties":[{"key":"GoTerm","value":"C:cell outer membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0009986","properties":[{"key":"GoTerm","value":"C:cell surface"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0042597","properties":[{"key":"GoTerm","value":"C:periplasmic space"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"Gene3D","id":"2.40.128.130","properties":[{"key":"EntryName","value":"Autotransporter beta-domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005546","properties":[{"key":"EntryName","value":"Autotransporte_beta"}]},{"database":"InterPro","id":"IPR036709","properties":[{"key":"EntryName","value":"Autotransporte_beta_dom_sf"}]},{"database":"InterPro","id":"IPR006315","properties":[{"key":"EntryName","value":"OM_autotransptr_brl_dom"}]},{"database":"NCBIfam","id":"TIGR01414","properties":[{"key":"EntryName","value":"autotrans_barl"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03797","properties":[{"key":"EntryName","value":"Autotransporter"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00869","properties":[{"key":"EntryName","value":"Autotransporter"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103515","properties":[{"key":"EntryName","value":"Autotransporter"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51208","properties":[{"key":"EntryName","value":"AUTOTRANSPORTER"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MANISPKLFKKAIQQGLKAALFTTSTAAIMLSSSGALGVATGVIATNNNAAFSNNVGNNNWNEITAAGVANGTPAGGPQNNWAFTYGGDYTVTADAADRIIKAINVAGTTPVGLNITQNTVVGSIITKGNLLPVTLNAGKSLTLNGNNAVAANHGFDAPADNYTGLGNIALGGANAALIIQSAAPSKITLAGNIDGGGIITVKTDAAINGTIGNTNALATVNVGAGTATLGGAVIKATTTKLTNAASVLTLTNANAVLTGAIDNTTGGDNVGVLNLNGALSQVTGDIGNTNSLATISVGAGTATLGGAVIKATTTKLTDAASAVKFTNPVVVTGAIDNTGNANNGIVTFTGNSTVTGNVGNTNALATVNVGAGLLQVQGGVVKANTINLTDNASAVTFTNPVVVTGAIDNTGNANNGIVTFTGNSTVTGDIGNTNALATVNVGAGTATLGGAVIKATTTKLTNAASVLTLTNANAVLTGAIDNTTGGDNVGVLNLNGALSQVTGNIGNTNSLATISVGAGTATLGGAVIKATTTKLTDAASAVKFTNPVVVTGAIDNTGNANNGIVTFTGNSTVTGDIGNTNSLATISVGAGTATLGGAVIKATTTKLTNAASVLTLTNANAVLTGAIDNTTGGDNVGVLNLNGALSQVTGDIGNTNSLATISVGAGTATLGGAVIKATTTKITNAVSAVKFTNPVVVTGAIDSTGNANNGIVTFTGNSTVTGDIGNTNALATVNVGAGTATLGGAVIKATTTKLTNAASVLTLTNANAVLTGAIDNTTGGDNVGVLNLNGALSQVTGDIGNTNSLATISVGAGTATLGGAVIKATTTKLTNAASVLTLTNANAVLTGAVDNTTGGDNVGVLNLNGALSQVTGDIGNTNSLATISVGAGTATLGGAVIKATTTKLTNAASVLTLTNANAVLTGAIDNTTGGDNVGVLNLNGALSQVTGDIGNTNSLATISVGAGTATLGGAVIKATTTKLTDAASAVKFTNPVVVTGAIDNTGNANNGIVTFTGNSTVTGNVGNTNALATVNVGAGLLQVQGGVVKANTINLTDNASAVTFTNPVVVTGAIDNTGNANNGIVTFTGNSTVTGNVGNTNALATVNVGAGLLQVQGGVVKANTINLTDNASAVTFTNPVVVTGAIDNTGNANNGIVTFTGNSTVTGDIGNTNALATVNVGAGITLQAGGSLAANNIDFGARSTLEFNGPLDGGGKAIPYYFKGAIANGNNAILNVNTKLLTASHLTIGTVAEINIGAGNLFTIDASVGDVTILNAQNINFRARDSVLVLSNLTGVGVNNILLAADLVAPGADEGTVVFNGGVNGLNVGSNVAGTARNIGDGGGNKFNTLLIYNAVTITDDVNLEGIQNVLINKNADFTSSTAFNAGAIQINDATYTIDANNGNLNIPAGNIQFAHADAQLVLQNSSGNDRTITLGANIDPDNDDEGIVILNSVTAGKKLTIAGGKTFGGAHKLQTILFKGAGDCSTAGTTFNTTNIVLDITGQLELGATTANVVLFNDAVQLTQTGNIGGFLDFNAKNGMVTLNNNVNVAGAVQNTGGTNNGTLIVLGASNLNRVNGIAMLKVGAGNVTIAKGGKVKIGEIQGTGTNTLTLPAHFNLTGSINKTGGQALKLNFMNGGSVSGVVGTAANSVGDITTAGATSFASSVNAKGTATLGGTTSFANTFTNTGAVTLAKGSITSFAKNVTATSFVANSATINFSNSLAFNSNITGGGTTLTLGANQVTYTGTGSFTDTLTLNTTFDGAAKSGGNILIKSGSTLDLSGVSTLALVVTATNFDMNNISPDTKYTVISAETAGGLKPTSKENVKITINNDNRFVDFTFDASTLTLFAEDIAADVIDGDFAPGGPLANIPNAANIKKSLELMEDAPNGSDARQAFNNFGLMTPLQEADATTHLIQDVVKPSDTIAAVNNQVVASNISSNITALNARMDKVQSGNKGPVSSGDEDMDAKFGAWISPFVGNATQKMCNSISGYKSDTTGGTIGFDGFVSDDLALGLAYTRADTDIKLKNNKTGDKNKVESNIYSLYGLYNVPYENLFVEAIASYSDNKIRSKSRRVIATTLETVGYQTANGKYKSESYTGQLMAGYTYMMPENINLTPLAGLRYSTIKDKGYKETGTTYQNLTVKGKNYNTFDGLLGAKVSSNINVNEIVLTPELYAMVDYAFKNKVSAIDARLQGMTAPLPTNSFKQSKTSFDVGVGVTAKHKMMEYRINYDTNIGSKYFAQQGSVKVRVNF","length":2249,"molWeight":224333,"crc64":"A9D6646C089DF087","md5":"3BB3CCECD975FE36890316D529552B2C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":3,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":3,"Repeat":14,"Domain":1,"Region":1},"uniParcId":"UPI0000130CF6"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P32199","uniProtkbId":"LPXA_RICRI","entryAudit":{"firstPublicDate":"1993-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1993-10-01","entryVersion":106,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00387"}],"value":"Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00387"}],"value":"UDP-N-acetylglucosamine acyltransferase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00387"}],"value":"2.3.1.129"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00387"}],"value":"lpxA"}}],"comments":[{"texts":[{"value":"Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a (3R)-hydroxyacyl-[ACP] + UDP-N-acetyl-alpha-D-glucosamine = a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine + holo-[ACP]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:67812"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"Rhea","id":"RHEA-COMP:9945"},{"database":"ChEBI","id":"CHEBI:57705"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78827"},{"database":"ChEBI","id":"CHEBI:173225"}],"ecNumber":"2.3.1.129","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00387"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00387"}],"value":"Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00387"}],"value":"Homotrimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00387"}],"value":"Belongs to the transferase hexapeptide repeat family. LpxA subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase","featureId":"PRO_0000188066"}],"keywords":[{"id":"KW-0012","category":"Molecular function","name":"Acyltransferase"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0441","category":"Biological process","name":"Lipid A biosynthesis"},{"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"8125326","citationType":"journal article","authors":["Shaw E.I.","Wood D.O."],"citationCrossReferences":[{"database":"PubMed","id":"8125326"},{"database":"DOI","id":"10.1016/0378-1119(94)90739-0"}],"title":"Characterization of a Rickettsia rickettsii DNA fragment analogous to the fir A-ORF17-lpxA region of Escherichia coli.","publicationDate":"1994","journal":"Gene","firstPage":"109","lastPage":"113","volume":"140"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L22690","properties":[{"key":"ProteinId","value":"AAA26386.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012150231.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"P32199","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P32199","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79936820","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ECVTINR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00359","properties":[{"key":"RectionId","value":"UER00477"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008780","properties":[{"key":"GoTerm","value":"F:acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009245","properties":[{"key":"GoTerm","value":"P:lipid A biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd03351","properties":[{"key":"EntryName","value":"LbH_UDP-GlcNAc_AT"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.160.10.10","properties":[{"key":"EntryName","value":"Hexapeptide repeat proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.1180.10","properties":[{"key":"EntryName","value":"Udp N-acetylglucosamine O-acyltransferase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00387","properties":[{"key":"EntryName","value":"LpxA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR029098","properties":[{"key":"EntryName","value":"Acetyltransf_C"}]},{"database":"InterPro","id":"IPR037157","properties":[{"key":"EntryName","value":"Acetyltransf_C_sf"}]},{"database":"InterPro","id":"IPR056729","properties":[{"key":"EntryName","value":"GMPPB_C"}]},{"database":"InterPro","id":"IPR001451","properties":[{"key":"EntryName","value":"Hexapep"}]},{"database":"InterPro","id":"IPR018357","properties":[{"key":"EntryName","value":"Hexapep_transf_CS"}]},{"database":"InterPro","id":"IPR010137","properties":[{"key":"EntryName","value":"Lipid_A_LpxA"}]},{"database":"InterPro","id":"IPR011004","properties":[{"key":"EntryName","value":"Trimer_LpxA-like_sf"}]},{"database":"NCBIfam","id":"TIGR01852","properties":[{"key":"EntryName","value":"lipid_A_lpxA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF003657","properties":[{"key":"EntryName","value":"PRK05289.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43480","properties":[{"key":"EntryName","value":"ACYL-[ACYL-CARRIER-PROTEIN]--UDP-N-ACETYLGLUCOSAMINE O-ACYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43480:SF1","properties":[{"key":"EntryName","value":"ACYL-[ACYL-CARRIER-PROTEIN]--UDP-N-ACETYLGLUCOSAMINE O-ACYLTRANSFERASE, MITOCHONDRIAL-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13720","properties":[{"key":"EntryName","value":"Acetyltransf_11"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25087","properties":[{"key":"EntryName","value":"GMPPB_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00132","properties":[{"key":"EntryName","value":"Hexapep"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000456","properties":[{"key":"EntryName","value":"UDP-GlcNAc_acltr"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51161","properties":[{"key":"EntryName","value":"Trimeric LpxA-like enzymes"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00101","properties":[{"key":"EntryName","value":"HEXAPEP_TRANSFERASES"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSNSNIHTTAVIAEGAKLGKNVKIGPYCIIGPEVVLNDNVELKSHVVIEGITEIGENTVIYPFASIGQPPQILKYANERSSTIIGSNNTIREYVTVQAGSQGGGMMTRVGNNNLFMVGVHIGHDCKIGNNVVFANYVSLAGHIGVGDYAIIGGLSAVHQYARIGEYSMIGGLSPVGADVIPFGLVSSKRAVLEGLNLIGMNRKGFDKVKSLSALKAIEEIFSGEGNFAERIKQVAEKYNNNSIVIQIIDFLNQDSSRAFCRFEK","length":264,"molWeight":28333,"crc64":"7A24B38C76A596D0","md5":"B19FC0EE70F2D7E2C7374F3C39B8244D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI000012E8BA"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O34198","uniProtkbId":"CH60_RICRI","entryAudit":{"firstPublicDate":"1998-12-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-01-01","entryVersion":98,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"Chaperonin GroEL"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"5.6.1.7"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"60 kDa chaperonin"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"Chaperonin-60"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"Cpn60"}]}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"groEL"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"groL"},{"value":"mopA"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP + H2O + an unfolded polypeptide = ADP + phosphate + a folded polypeptide.","ecNumber":"5.6.1.7","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the chaperonin (HSP60) family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":408,"modifier":"OUTSIDE"}},"description":"Chaperonin GroEL","featureId":"PRO_0000063517"},{"type":"Binding site","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00600"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Non-terminal residue","location":{"start":{"value":408,"modifier":"EXACT"},"end":{"value":408,"modifier":"EXACT"}},"description":""}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0143","category":"Molecular function","name":"Chaperone"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"9230387","citationType":"journal article","authors":["Sumner J.W.","Nicholson W.L.","Massung R.F."],"citationCrossReferences":[{"database":"PubMed","id":"9230387"},{"database":"DOI","id":"10.1128/jcm.35.8.2087-2092.1997"}],"title":"PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species.","publicationDate":"1997","journal":"J. Clin. Microbiol.","firstPage":"2087","lastPage":"2092","volume":"35"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"R","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U96733","properties":[{"key":"ProteinId","value":"AAB65635.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"O34198","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O34198","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0140662","properties":[{"key":"GoTerm","value":"F:ATP-dependent protein folding chaperone"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0042026","properties":[{"key":"GoTerm","value":"P:protein refolding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd03344","properties":[{"key":"EntryName","value":"GroEL"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.50.7.10:FF:000001","properties":[{"key":"EntryName","value":"60 kDa chaperonin"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.50.7.10","properties":[{"key":"EntryName","value":"GroEL"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.560.10","properties":[{"key":"EntryName","value":"GroEL-like equatorial domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.260.10","properties":[{"key":"EntryName","value":"TCP-1-like chaperonin intermediate domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001844","properties":[{"key":"EntryName","value":"Cpn60/GroEL"}]},{"database":"InterPro","id":"IPR002423","properties":[{"key":"EntryName","value":"Cpn60/GroEL/TCP-1"}]},{"database":"InterPro","id":"IPR027409","properties":[{"key":"EntryName","value":"GroEL-like_apical_dom_sf"}]},{"database":"InterPro","id":"IPR027413","properties":[{"key":"EntryName","value":"GROEL-like_equatorial_sf"}]},{"database":"InterPro","id":"IPR027410","properties":[{"key":"EntryName","value":"TCP-1-like_intermed_sf"}]},{"database":"NCBIfam","id":"TIGR02348","properties":[{"key":"EntryName","value":"GroEL"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF000592","properties":[{"key":"EntryName","value":"PRK00013.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009487","properties":[{"key":"EntryName","value":"PRK12849.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009488","properties":[{"key":"EntryName","value":"PRK12850.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009489","properties":[{"key":"EntryName","value":"PRK12851.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45633","properties":[{"key":"EntryName","value":"60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00118","properties":[{"key":"EntryName","value":"Cpn60_TCP1"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00298","properties":[{"key":"EntryName","value":"CHAPERONIN60"}]},{"database":"SUPFAM","id":"SSF52029","properties":[{"key":"EntryName","value":"GroEL apical domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48592","properties":[{"key":"EntryName","value":"GroEL equatorial domain-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MATKLIKHGSKAREQMLEGIDILADAVKVTLGPKGRNVLIEQSFGSPKITKDGVTVAKSIELKDKIRNAGAQLLKSAATKAAEVAGDGTTTATVLARALAREGNKLVAAGYNPMDLKRGMDLAVNAVVEEIKKSSKKINSQEEIAQVGTISSNGDKEIGEKIAKAMEEVGKEGVITVEEAKNFSFDVEVVKGMMFDRGYLSPYFVTNSEKMVAELENPFILLFEKKLSNLQPMLPILEAVVQSQRPLLIIAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMMEDIAILTKGELITEDLGMKLENVSIKSLGTAKRVTISKENTVIVDGNGDKKNIEDRVLQIKSQIAETTSDYDKEKLQERLAKLSGGVAVLKVGGATEVEVKERKDRVEDALAATRAAVE","length":408,"molWeight":43986,"crc64":"EFEA390CA721F78E","md5":"5E1567B879671A43D680B736ED589F0A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":3,"Non-terminal residue":1},"uniParcId":"UPI000012770C"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0A3A9","secondaryAccessions":["Q8KI92"],"uniProtkbId":"EFTU_RICRI","entryAudit":{"firstPublicDate":"2005-03-15","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2005-03-15","entryVersion":78,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"value":"Elongation factor Tu"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"value":"EF-Tu"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"value":"3.6.5.3"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"value":"tuf"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"value":"GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"GTP + H2O = GDP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19669"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58189"}],"ecNumber":"3.6.5.3","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:19670"},"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":394,"modifier":"EXACT"}},"description":"Elongation factor Tu","featureId":"PRO_0000091380"},{"type":"Domain","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":204,"modifier":"EXACT"}},"description":"tr-type G"},{"type":"Region","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"G1","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Region","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"G2","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Region","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"G3","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Region","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"G4","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Region","location":{"start":{"value":174,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"G5","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Binding site","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00118"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0251","category":"Molecular function","name":"Elongation factor"},{"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"12140235","citationType":"journal article","authors":["Amiri H.","Alsmark C.M.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"12140235"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a004184"}],"title":"Proliferation and deterioration of Rickettsia palindromic elements.","publicationDate":"2002","journal":"Mol. Biol. Evol.","firstPage":"1234","lastPage":"1243","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF502179","properties":[{"key":"ProteinId","value":"AAM90930.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"P0A3A9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0A3A9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003746","properties":[{"key":"GoTerm","value":"F:translation elongation factor activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd01884","properties":[{"key":"EntryName","value":"EF_Tu"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03697","properties":[{"key":"EntryName","value":"EFTU_II"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03707","properties":[{"key":"EntryName","value":"EFTU_III"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.30.10:FF:000001","properties":[{"key":"EntryName","value":"Elongation factor Tu"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000003","properties":[{"key":"EntryName","value":"Elongation factor Tu"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"2"}]},{"database":"HAMAP","id":"MF_00118_B","properties":[{"key":"EntryName","value":"EF_Tu_B"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR041709","properties":[{"key":"EntryName","value":"EF-Tu_GTP-bd"}]},{"database":"InterPro","id":"IPR050055","properties":[{"key":"EntryName","value":"EF-Tu_GTPase"}]},{"database":"InterPro","id":"IPR004161","properties":[{"key":"EntryName","value":"EFTu-like_2"}]},{"database":"InterPro","id":"IPR033720","properties":[{"key":"EntryName","value":"EFTU_2"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR005225","properties":[{"key":"EntryName","value":"Small_GTP-bd"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR009001","properties":[{"key":"EntryName","value":"Transl_elong_EF1A/Init_IF2_C"}]},{"database":"InterPro","id":"IPR004541","properties":[{"key":"EntryName","value":"Transl_elong_EFTu/EF1A_bac/org"}]},{"database":"InterPro","id":"IPR004160","properties":[{"key":"EntryName","value":"Transl_elong_EFTu/EF1A_C"}]},{"database":"NCBIfam","id":"TIGR00485","properties":[{"key":"EntryName","value":"EF-Tu"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF000766","properties":[{"key":"EntryName","value":"PRK00049.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009372","properties":[{"key":"EntryName","value":"PRK12735.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009373","properties":[{"key":"EntryName","value":"PRK12736.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00231","properties":[{"key":"EntryName","value":"small_GTP"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43721:SF22","properties":[{"key":"EntryName","value":"ELONGATION FACTOR TU, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43721","properties":[{"key":"EntryName","value":"ELONGATION FACTOR TU-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03144","properties":[{"key":"EntryName","value":"GTP_EFTU_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03143","properties":[{"key":"EntryName","value":"GTP_EFTU_D3"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"SUPFAM","id":"SSF50465","properties":[{"key":"EntryName","value":"EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAKAKFERTKPHVNIGTIGHVDHGKTSLTAAITIVLAKTGGAQATAYDQIDAAPEEKERGITISTAHVEYETKNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPAMVVFLNKIDMVDDPDLLELVEMEVRELLSKYGFPGDEIPIIKGSALQALEGKPEGEKAINELMDAVDSYIPQPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVGEEIEIVGLKDTQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIKPHDKFEAEVYVLSKEEGGRHTPFTNDYRPQFYFRTTDVTGTIKLPADKQWVMPGDNATFTVELIKPIAMQEGLKFSIREGGRTVGAGVVTKINN","length":394,"molWeight":42933,"crc64":"139D7A0049C9BEC3","md5":"FCCEE31D65C46ADA837C010FF306E70F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Region":5,"Binding site":4},"uniParcId":"UPI00000B2C13"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9AKI7","uniProtkbId":"MURE_RICRI","entryAudit":{"firstPublicDate":"2003-08-22","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":108,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"6.3.2.13"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Meso-A2pm-adding enzyme"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Meso-diaminopimelate-adding enzyme"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"UDP-MurNAc-tripeptide synthetase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"UDP-N-acetylmuramyl-tripeptide synthetase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"murE"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminopimelate + ATP = UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelate + ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23676"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57791"},{"database":"ChEBI","id":"CHEBI:83900"},{"database":"ChEBI","id":"CHEBI:83905"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"6.3.2.13","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Cell wall biogenesis; peptidoglycan biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Belongs to the MurCDEF family. MurE subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":479,"modifier":"EXACT"}},"description":"UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase","featureId":"PRO_0000101935"},{"type":"Motif","location":{"start":{"value":396,"modifier":"EXACT"},"end":{"value":399,"modifier":"EXACT"}},"description":"Meso-diaminopimelate recognition motif"},{"type":"Binding site","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:83900"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate","id":"ChEBI:CHEBI:83900"}},{"type":"Binding site","location":{"start":{"value":98,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":144,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:83900"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate","id":"ChEBI:CHEBI:83900"}},{"type":"Binding site","location":{"start":{"value":171,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:83900"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate","id":"ChEBI:CHEBI:83900"}},{"type":"Binding site","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:83900"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate","id":"ChEBI:CHEBI:83900"}},{"type":"Binding site","location":{"start":{"value":179,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:83900"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate","id":"ChEBI:CHEBI:83900"}},{"type":"Binding site","location":{"start":{"value":372,"modifier":"EXACT"},"end":{"value":372,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57791"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"meso-2,6-diaminopimelate","id":"ChEBI:CHEBI:57791"}},{"type":"Binding site","location":{"start":{"value":396,"modifier":"EXACT"},"end":{"value":399,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57791"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"meso-2,6-diaminopimelate","id":"ChEBI:CHEBI:57791"}},{"type":"Binding site","location":{"start":{"value":446,"modifier":"EXACT"},"end":{"value":446,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57791"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"meso-2,6-diaminopimelate","id":"ChEBI:CHEBI:57791"}},{"type":"Binding site","location":{"start":{"value":450,"modifier":"EXACT"},"end":{"value":450,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57791"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"meso-2,6-diaminopimelate","id":"ChEBI:CHEBI:57791"}},{"type":"Modified residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"N6-carboxylysine","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00208"}]}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"id":"KW-0132","category":"Biological process","name":"Cell division"},{"id":"KW-0133","category":"Biological process","name":"Cell shape"},{"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0573","category":"Biological process","name":"Peptidoglycan synthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"11319266","citationType":"journal article","authors":["Andersson J.O.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"11319266"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a003864"}],"title":"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes.","publicationDate":"2001","journal":"Mol. Biol. Evol.","firstPage":"829","lastPage":"839","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"84-21C","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ293314","properties":[{"key":"ProteinId","value":"CAC33671.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012151073.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"Q9AKI7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9AKI7","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937591","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"EYFIMEV","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00219","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008765","properties":[{"key":"GoTerm","value":"F:UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051301","properties":[{"key":"GoTerm","value":"P:cell division"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0009252","properties":[{"key":"GoTerm","value":"P:peptidoglycan biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008360","properties":[{"key":"GoTerm","value":"P:regulation of cell shape"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.90.190.20","properties":[{"key":"EntryName","value":"Mur ligase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1190.10","properties":[{"key":"EntryName","value":"Mur-like, catalytic domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1390.10","properties":[{"key":"EntryName","value":"MurE/MurF, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00208","properties":[{"key":"EntryName","value":"MurE"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036565","properties":[{"key":"EntryName","value":"Mur-like_cat_sf"}]},{"database":"InterPro","id":"IPR004101","properties":[{"key":"EntryName","value":"Mur_ligase_C"}]},{"database":"InterPro","id":"IPR036615","properties":[{"key":"EntryName","value":"Mur_ligase_C_dom_sf"}]},{"database":"InterPro","id":"IPR013221","properties":[{"key":"EntryName","value":"Mur_ligase_cen"}]},{"database":"InterPro","id":"IPR000713","properties":[{"key":"EntryName","value":"Mur_ligase_N"}]},{"database":"InterPro","id":"IPR035911","properties":[{"key":"EntryName","value":"MurE/MurF_N"}]},{"database":"InterPro","id":"IPR005761","properties":[{"key":"EntryName","value":"UDP-N-AcMur-Glu-dNH2Pim_ligase"}]},{"database":"NCBIfam","id":"TIGR01085","properties":[{"key":"EntryName","value":"murE"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF001124","properties":[{"key":"EntryName","value":"PRK00139.1-2"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF001126","properties":[{"key":"EntryName","value":"PRK00139.1-4"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR23135","properties":[{"key":"EntryName","value":"MUR LIGASE FAMILY MEMBER"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR23135:SF4","properties":[{"key":"EntryName","value":"UDP-N-ACETYLMURAMOYL-L-ALANYL-D-GLUTAMATE--2,6-DIAMINOPIMELATE LIGASE MURE HOMOLOG, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01225","properties":[{"key":"EntryName","value":"Mur_ligase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02875","properties":[{"key":"EntryName","value":"Mur_ligase_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08245","properties":[{"key":"EntryName","value":"Mur_ligase_M"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53623","properties":[{"key":"EntryName","value":"MurD-like peptide ligases, catalytic domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53244","properties":[{"key":"EntryName","value":"MurD-like peptide ligases, peptide-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF63418","properties":[{"key":"EntryName","value":"MurE/MurF N-terminal domain"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSHNLKQLFQQHNVKGLSINSKTVKDKDIFFAIKGRNTDGNDFIKDALSKGAVLVITDNKKNIVIDKVIYVKDVQAALYEAIEIFYPKKPKDLIAVTGTNGKSSVVSYIAQTYSLLGKKAASIGTIGVEIFGCVNLINDVPELTTLDYLSFRKIAHNLAENGIEYLVFEASSHGLDQARLREIKVNIACFTSFSQDHLDYHHTKENYLLAKLKLFINHLLPNGIAILNSDIEEIEFVKDYLHNHNVKFITVGTKGDLEITRLNCSLKGQNINFTFNNREYNFNTPIIGSFQASNLLIAVLSIHYIGFAFDDVIDSLVEVKAVKGRMERIDNTNIFVDYAHTPDALEKALTELKNIKLRDSKLSVVFGCGGNRDKAKRSLMGQIAAKRADTIIITDDNPRHEDPKLIRAEIISGIEKADYTEIANREEAIKYGINNLKQDDILLVAGKGHENYQIIGDKKLPFDDAEVVRKCIKVCHPVA","length":479,"molWeight":53640,"crc64":"BE5379B53EC28326","md5":"A7AF0E44541627A8FE9F70835C17E712"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Motif":1,"Binding site":10,"Modified residue":1},"uniParcId":"UPI00000BA2C0"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9AKI9","uniProtkbId":"MRAY_RICRI","entryAudit":{"firstPublicDate":"2003-02-28","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":94,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}],"value":"Phospho-N-acetylmuramoyl-pentapeptide-transferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}],"value":"2.7.8.13"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}],"value":"UDP-MurNAc-pentapeptide phosphotransferase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}],"value":"mraY"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}],"value":"Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + di-trans,octa-cis-undecaprenyl phosphate = di-trans,octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:28386"},{"database":"ChEBI","id":"CHEBI:57865"},{"database":"ChEBI","id":"CHEBI:60392"},{"database":"ChEBI","id":"CHEBI:61386"},{"database":"ChEBI","id":"CHEBI:61387"}],"ecNumber":"2.7.8.13","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}],"value":"Cell wall biogenesis; peptidoglycan biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase 4 family. MraY subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":361,"modifier":"EXACT"}},"description":"Phospho-N-acetylmuramoyl-pentapeptide-transferase","featureId":"PRO_0000108881"},{"type":"Transmembrane","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]},{"type":"Transmembrane","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]},{"type":"Transmembrane","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]},{"type":"Transmembrane","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]},{"type":"Transmembrane","location":{"start":{"value":168,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]},{"type":"Transmembrane","location":{"start":{"value":203,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]},{"type":"Transmembrane","location":{"start":{"value":236,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]},{"type":"Transmembrane","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]},{"type":"Transmembrane","location":{"start":{"value":288,"modifier":"EXACT"},"end":{"value":308,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]},{"type":"Transmembrane","location":{"start":{"value":338,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00038"}]}],"keywords":[{"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"id":"KW-0132","category":"Biological process","name":"Cell division"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0133","category":"Biological process","name":"Cell shape"},{"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0573","category":"Biological process","name":"Peptidoglycan synthesis"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"11319266","citationType":"journal article","authors":["Andersson J.O.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"11319266"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a003864"}],"title":"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes.","publicationDate":"2001","journal":"Mol. Biol. Evol.","firstPage":"829","lastPage":"839","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"84-21C","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ293314","properties":[{"key":"ProteinId","value":"CAC33669.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012151071.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"Q9AKI9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9AKI9","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937589","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"DTPTMGG","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00219","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008963","properties":[{"key":"GoTerm","value":"F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051992","properties":[{"key":"GoTerm","value":"F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"},{"key":"GoEvidenceType","value":"IEA:RHEA"}]},{"database":"GO","id":"GO:0071555","properties":[{"key":"GoTerm","value":"P:cell wall organization"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009252","properties":[{"key":"GoTerm","value":"P:peptidoglycan biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd06852","properties":[{"key":"EntryName","value":"GT_MraY"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00038","properties":[{"key":"EntryName","value":"MraY"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR000715","properties":[{"key":"EntryName","value":"Glycosyl_transferase_4"}]},{"database":"InterPro","id":"IPR003524","properties":[{"key":"EntryName","value":"PNAcMuramoyl-5peptid_Trfase"}]},{"database":"InterPro","id":"IPR018480","properties":[{"key":"EntryName","value":"PNAcMuramoyl-5peptid_Trfase_CS"}]},{"database":"NCBIfam","id":"TIGR00445","properties":[{"key":"EntryName","value":"mraY"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR22926","properties":[{"key":"EntryName","value":"PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR22926:SF5","properties":[{"key":"EntryName","value":"PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE HOMOLOG"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00953","properties":[{"key":"EntryName","value":"Glycos_transf_4"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01347","properties":[{"key":"EntryName","value":"MRAY_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01348","properties":[{"key":"EntryName","value":"MRAY_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLYNLLLPHIHNSHIANLFHYITFRSGLAIIITLSLSFITGPILIEFLRSIQKNGQPIRSDGPESHQTKVGTPTMGGIMIILSSCLSTLLLADLTNKYIWITLFGFISFGIIGFMDDYAKVTKNNHYGVRGKSKLLLQGIISVIICVLLEYLDKSPSHLLNVPFFKNLSLDLGYCYIVFAIFVIVGSSNAVNLTDGLDGLATVPIAFTAGSFALISYLVGNLIYSNYLQLTYIPNTGELTVLCAGLVGSCLGFLWFNAQPAEVFMGDTGSLSLGGVLGIISVITKHEIVLAIVGGLFVIETASVILQVYYFKATKGKRIFKMAPLHHHFEKHGWAESKVVIRFWIISVIFALIGLSSLKLR","length":361,"molWeight":39624,"crc64":"83369108375F0CDB","md5":"65A9B28A314B31E746ADB2432201732F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":10},"uniParcId":"UPI000012F529"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9AKJ8","uniProtkbId":"SSRP_RICRI","entryAudit":{"firstPublicDate":"2003-04-23","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2001-06-01","entryVersion":80,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00023"}],"value":"SsrA-binding protein"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00023"}],"value":"Small protein B"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00023"}],"value":"smpB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00023"}],"value":"Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00023"}],"value":"The tmRNA-SmpB complex associates with stalled 70S ribosomes"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00023"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00023"}],"value":"Belongs to the SmpB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"SsrA-binding protein","featureId":"PRO_0000103019"}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"11319266","citationType":"journal article","authors":["Andersson J.O.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"11319266"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a003864"}],"title":"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes.","publicationDate":"2001","journal":"Mol. Biol. Evol.","firstPage":"829","lastPage":"839","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"84-21C","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ293311","properties":[{"key":"ProteinId","value":"CAC33658.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012150787.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"Q9AKJ8","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9AKJ8","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937340","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"WTNHSAR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003723","properties":[{"key":"GoTerm","value":"F:RNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0070929","properties":[{"key":"GoTerm","value":"P:trans-translation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0070930","properties":[{"key":"GoTerm","value":"P:trans-translation-dependent protein tagging"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd09294","properties":[{"key":"EntryName","value":"SmpB"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.280.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00023","properties":[{"key":"EntryName","value":"SmpB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR023620","properties":[{"key":"EntryName","value":"SmpB"}]},{"database":"InterPro","id":"IPR000037","properties":[{"key":"EntryName","value":"SsrA-bd_prot"}]},{"database":"NCBIfam","id":"NF003843","properties":[{"key":"EntryName","value":"PRK05422.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00086","properties":[{"key":"EntryName","value":"smpB"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30308:SF2","properties":[{"key":"EntryName","value":"SSRA-BINDING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30308","properties":[{"key":"EntryName","value":"TMRNA-BINDING COMPONENT OF TRANS-TRANSLATION TAGGING COMPLEX"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01668","properties":[{"key":"EntryName","value":"SmpB"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF74982","properties":[{"key":"EntryName","value":"Small protein B (SmpB)"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTEYKKVIAQNKKALFHYFIEERLEAGIVLKGSEVRSLRQGKASIEESHAADTGHEVFLYNCHIAEYEKANRFNHATRRPRKLLLHTKEIKKIIGRIRIKGYTLVALSMYFNKKNKVKVELGIAKGKKLHDKRESIKEKDWKRDQSRLIRQK","length":152,"molWeight":17903,"crc64":"7DFBDA78DB5BE808","md5":"F2D674151AB3C7B3B3805F98A573CE85"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI00000BD8D2"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P32202","uniProtkbId":"LPXD_RICRI","entryAudit":{"firstPublicDate":"1993-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1993-10-01","entryVersion":93,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00523"}],"value":"UDP-3-O-acylglucosamine N-acyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00523"}],"value":"2.3.1.191"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00523"}],"value":"lpxD"},"synonyms":[{"value":"firA"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00523"}],"value":"Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + a (3R)-hydroxyacyl-[ACP] = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + holo-[ACP] + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:53836"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"Rhea","id":"RHEA-COMP:9945"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78827"},{"database":"ChEBI","id":"CHEBI:137740"},{"database":"ChEBI","id":"CHEBI:137748"}],"ecNumber":"2.3.1.191","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00523"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00523"}],"value":"Bacterial outer membrane biogenesis; LPS lipid A biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00523"}],"value":"Homotrimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00523"}],"value":"Belongs to the transferase hexapeptide repeat family. LpxD subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"UDP-3-O-acylglucosamine N-acyltransferase","featureId":"PRO_0000059699"},{"type":"Active site","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00523"}]}],"keywords":[{"id":"KW-0012","category":"Molecular function","name":"Acyltransferase"},{"id":"KW-0441","category":"Biological process","name":"Lipid A biosynthesis"},{"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"8125326","citationType":"journal article","authors":["Shaw E.I.","Wood D.O."],"citationCrossReferences":[{"database":"PubMed","id":"8125326"},{"database":"DOI","id":"10.1016/0378-1119(94)90739-0"}],"title":"Characterization of a Rickettsia rickettsii DNA fragment analogous to the fir A-ORF17-lpxA region of Escherichia coli.","publicationDate":"1994","journal":"Gene","firstPage":"109","lastPage":"113","volume":"140"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L22690","properties":[{"key":"ProteinId","value":"AAA26384.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012150232.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"P32202","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P32202","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79936822","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PAMEIHE","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00973","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0103118","properties":[{"key":"GoTerm","value":"F:UDP-3-O-[(3R)-3-hydroxyacyl]-glucosamine N-acyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0009245","properties":[{"key":"GoTerm","value":"P:lipid A biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd03352","properties":[{"key":"EntryName","value":"LbH_LpxD"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.160.10.10","properties":[{"key":"EntryName","value":"Hexapeptide repeat proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1390.10","properties":[{"key":"EntryName","value":"MurE/MurF, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00523","properties":[{"key":"EntryName","value":"LpxD"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001451","properties":[{"key":"EntryName","value":"Hexapep"}]},{"database":"InterPro","id":"IPR018357","properties":[{"key":"EntryName","value":"Hexapep_transf_CS"}]},{"database":"InterPro","id":"IPR007691","properties":[{"key":"EntryName","value":"LpxD"}]},{"database":"InterPro","id":"IPR011004","properties":[{"key":"EntryName","value":"Trimer_LpxA-like_sf"}]},{"database":"InterPro","id":"IPR020573","properties":[{"key":"EntryName","value":"UDP_GlcNAc_AcTrfase_non-rep"}]},{"database":"NCBIfam","id":"TIGR01853","properties":[{"key":"EntryName","value":"lipid_A_lpxD"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF002060","properties":[{"key":"EntryName","value":"PRK00892.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43378","properties":[{"key":"EntryName","value":"UDP-3-O-ACYLGLUCOSAMINE N-ACYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43378:SF2","properties":[{"key":"EntryName","value":"UDP-3-O-ACYLGLUCOSAMINE N-ACYLTRANSFERASE 1, MITOCHONDRIAL-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00132","properties":[{"key":"EntryName","value":"Hexapep"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04613","properties":[{"key":"EntryName","value":"LpxD"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51161","properties":[{"key":"EntryName","value":"Trimeric LpxA-like enzymes"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00101","properties":[{"key":"EntryName","value":"HEXAPEP_TRANSFERASES"},{"key":"MatchStatus","value":"2"}]}],"sequence":{"value":"MVSSNFYKNLGPRKLTAIIDFLHDIIAPPKIEDIAIHDIKILQEASPNDISFLSNPKYSEFLKTTKAAACIVPKNFTGEANPNTVLLHAQNSYFAYGKLIDFFYAPIKSYPTKIMKSAIVADSATIGKNCYIGHNVVIEDDVIIGDNSIIEAGSFIGRGVNIGRNARIEQHVSINYAIIGDDVVILAGAKIGQDGFGFSTEKGVHHKIFHIGIVKIGNNVEIGANTTIDRGSLQDTIIKDLCRIDNLVQIGHGVKIGKGSIIVAQTGIAGSSTIGKYCALGGQVGIAGHLNIGDGAQVAAQGGVAQNIEAGKIVGGSPAIPIMDWHRQSIIMKQLLKTSNSKLKK","length":345,"molWeight":36794,"crc64":"41577509BD4D9CDF","md5":"8AE573FE84E56E6814C084F7044A751D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1},"uniParcId":"UPI000012E904"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0A3N5","secondaryAccessions":["P05372"],"uniProtkbId":"17KD_RICRI","entryAudit":{"firstPublicDate":"2005-03-15","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2005-03-15","entryVersion":51,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"17 kDa surface antigen"}},"flag":"Precursor"},"genes":[{"geneName":{"value":"omp"}}],"comments":[{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell outer membrane","id":"SL-0040"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Lipid-anchor","id":"SL-9901"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the rickettsiale 17 kDa surface antigen family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Chain","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"17 kDa surface antigen","featureId":"PRO_0000018022"},{"type":"Lipidation","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"N-palmitoyl cysteine","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Lipidation","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"S-diacylglycerol cysteine","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Sequence conflict","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"in Ref. 2; AAA26381","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["D"]}},{"type":"Sequence conflict","location":{"start":{"value":153,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"in Ref. 2; AAA26381","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["E"]}}],"keywords":[{"id":"KW-0998","category":"Cellular component","name":"Cell outer membrane"},{"id":"KW-0449","category":"PTM","name":"Lipoprotein"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0564","category":"PTM","name":"Palmitate"},{"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"3108232","citationType":"journal article","authors":["Anderson B.E.","Regnery R.L.","Carlone G.M.","Tzianabos T.","McDade J.E.","Fu Z.Y.","Bellini W.J."],"citationCrossReferences":[{"database":"PubMed","id":"3108232"},{"database":"DOI","id":"10.1128/jb.169.6.2385-2390.1987"}],"title":"Sequence analysis of the 17-kilodalton-antigen gene from Rickettsia rickettsii.","publicationDate":"1987","journal":"J. Bacteriol.","firstPage":"2385","lastPage":"2390","volume":"169"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"2768201","citationType":"journal article","authors":["Anderson B.E.","Tzianabos T."],"citationCrossReferences":[{"database":"PubMed","id":"2768201"},{"database":"DOI","id":"10.1128/jb.171.9.5199-5201.1989"}],"title":"Comparative sequence analysis of a genus-common rickettsial antigen gene.","publicationDate":"1989","journal":"J. Bacteriol.","firstPage":"5199","lastPage":"5201","volume":"171"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"3139629","citationType":"journal article","authors":["Anderson B.E.","Baumstark B.R.","Bellini W.J."],"citationCrossReferences":[{"database":"PubMed","id":"3139629"},{"database":"DOI","id":"10.1128/jb.170.10.4493-4500.1988"}],"title":"Expression of the gene encoding the 17-kilodalton antigen from Rickettsia rickettsii: transcription and posttranslational modification.","publicationDate":"1988","journal":"J. Bacteriol.","firstPage":"4493","lastPage":"4500","volume":"170"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M16486","properties":[{"key":"ProteinId","value":"AAA26381.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"M28479","properties":[{"key":"ProteinId","value":"AAA26379.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"J03371","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A25972","properties":[{"key":"EntryName","value":"A25972"}]},{"database":"PIR","id":"A31836","properties":[{"key":"EntryName","value":"A31836"}]},{"database":"PIR","id":"B33971","properties":[{"key":"EntryName","value":"B33971"}]},{"database":"RefSeq","id":"WP_004997204.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"P0A3N5","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937889","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"NCRQYTH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0009279","properties":[{"key":"GoTerm","value":"C:cell outer membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"InterPro","id":"IPR032635","properties":[{"key":"EntryName","value":"Anti_2"}]},{"database":"InterPro","id":"IPR008816","properties":[{"key":"EntryName","value":"Gly_zipper_2TM_dom"}]},{"database":"InterPro","id":"IPR016364","properties":[{"key":"EntryName","value":"Surface_antigen_Rickettsia"}]},{"database":"Pfam","id":"PF16998","properties":[{"key":"EntryName","value":"17kDa_Anti_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05433","properties":[{"key":"EntryName","value":"Rick_17kDa_Anti"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF002721","properties":[{"key":"EntryName","value":"Surface_antigen_Rickettsia"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51257","properties":[{"key":"EntryName","value":"PROKAR_LIPOPROTEIN"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKLLSKIMIIALATSMLQACNGPGGMNKQGTGTLLGGAGGALLGSQFGKGKGQLVGVGVGALLGAVLGGQIGAGMDEQDRRLAELTSQRALETAPSGSNVEWRNPDNGNYGYVTPNKTYRNSTGQYCREYTQTVVIGGKQQKAYGNACRQPDGQWQVVN","length":159,"molWeight":16581,"crc64":"206A2BBF74FCE169","md5":"A20A7910A7D5322F32743A0BFA72F756"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Lipidation":2,"Sequence conflict":2},"uniParcId":"UPI0000124E11"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P58187","uniProtkbId":"LPXK_RICRI","entryAudit":{"firstPublicDate":"2001-06-20","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2001-06-20","entryVersion":74,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00409"}],"value":"Tetraacyldisaccharide 4'-kinase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00409"}],"value":"2.7.1.130"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00409"}],"value":"Lipid A 4'-kinase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00409"}],"value":"lpxK"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00409"}],"value":"Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a lipid A disaccharide + ATP = a lipid IVA + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:67840"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:176343"},{"database":"ChEBI","id":"CHEBI:176425"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.1.130","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00409"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00409"}],"value":"Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00409"}],"value":"Belongs to the LpxK family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"Tetraacyldisaccharide 4'-kinase","featureId":"PRO_0000190947"},{"type":"Binding site","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00409"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"id":"KW-0441","category":"Biological process","name":"Lipid A biosynthesis"},{"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"11319266","citationType":"journal article","authors":["Andersson J.O.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"11319266"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a003864"}],"title":"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes.","publicationDate":"2001","journal":"Mol. Biol. Evol.","firstPage":"829","lastPage":"839","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"84-21C","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ293329","properties":[{"key":"ProteinId","value":"CAC33716.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012151226.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"P58187","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P58187","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937740","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"RAFPDHH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00359","properties":[{"key":"RectionId","value":"UER00482"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009029","properties":[{"key":"GoTerm","value":"F:lipid-A 4'-kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009245","properties":[{"key":"GoTerm","value":"P:lipid A biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009244","properties":[{"key":"GoTerm","value":"P:lipopolysaccharide core region biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"HAMAP","id":"MF_00409","properties":[{"key":"EntryName","value":"LpxK"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003758","properties":[{"key":"EntryName","value":"LpxK"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"NCBIfam","id":"TIGR00682","properties":[{"key":"EntryName","value":"lpxK"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42724","properties":[{"key":"EntryName","value":"TETRAACYLDISACCHARIDE 4'-KINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42724:SF1","properties":[{"key":"EntryName","value":"TETRAACYLDISACCHARIDE 4'-KINASE, MITOCHONDRIAL-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02606","properties":[{"key":"EntryName","value":"LpxK"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIKLLYPEFWQKRNIIAYLLLPISLIYQFLGYLRASLARPIMLPAKVICVGNCSVGGTGKTQIVMYLAKLLKARNVSFVIVTKAYGSNLKSATTIHQGHTALEVGDEGVILAKYGAVIATKNIKEIVPLINELKPDIIIVDDFLQNPYFHKDFTIVSVDSQRLFGNGFLIPAGPLRQYPNKALDAADLIFLVSSHQDKIPQILTPYVNKLINAQIVPSNNIDKTKNYFAFSGIGNPERFFATLKNYGLNITGYKIFPDHYNYLQADLENLYSLAKEHNAILVTTRKDHVKFNDLNNNIVCLDVELSINHPDLLNEKIFKKA","length":321,"molWeight":36083,"crc64":"45E6CEB6CC50C0AE","md5":"A223A1A25916EADAC69BEB5A2A780B7D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":1},"uniParcId":"UPI000012E92F"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8KTC1","uniProtkbId":"EFG_RICRI","entryAudit":{"firstPublicDate":"2003-08-22","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2002-10-01","entryVersion":98,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"Elongation factor G"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"EF-G"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"fusA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":699,"modifier":"EXACT"}},"description":"Elongation factor G","featureId":"PRO_0000091203"},{"type":"Domain","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"tr-type G"},{"type":"Binding site","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":135,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0251","category":"Molecular function","name":"Elongation factor"},{"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"12140235","citationType":"journal article","authors":["Amiri H.","Alsmark C.M.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"12140235"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a004184"}],"title":"Proliferation and deterioration of Rickettsia palindromic elements.","publicationDate":"2002","journal":"Mol. Biol. Evol.","firstPage":"1234","lastPage":"1243","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"Sawtooth","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF502170","properties":[{"key":"ProteinId","value":"AAM90913.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q8KTC1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8KTC1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0097216","properties":[{"key":"GoTerm","value":"F:guanosine tetraphosphate binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0003746","properties":[{"key":"GoTerm","value":"F:translation elongation factor activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd01886","properties":[{"key":"EntryName","value":"EF-G"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16262","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01434","properties":[{"key":"EntryName","value":"EFG_mtEFG1_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03713","properties":[{"key":"EntryName","value":"EFG_mtEFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04088","properties":[{"key":"EntryName","value":"EFG_mtEFG_II"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.30.10:FF:000006","properties":[{"key":"EntryName","value":"Elongation factor G"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.230.10:FF:000003","properties":[{"key":"EntryName","value":"Elongation factor G"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.240:FF:000001","properties":[{"key":"EntryName","value":"Elongation factor G"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.870:FF:000001","properties":[{"key":"EntryName","value":"Elongation factor G"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000029","properties":[{"key":"EntryName","value":"Elongation factor G"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.870","properties":[{"key":"EntryName","value":"Elongation Factor G (Translational Gtpase), domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00054_B","properties":[{"key":"EntryName","value":"EF_G_EF_2_B"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR053905","properties":[{"key":"EntryName","value":"EF-G-like_DII"}]},{"database":"InterPro","id":"IPR041095","properties":[{"key":"EntryName","value":"EFG_II"}]},{"database":"InterPro","id":"IPR009022","properties":[{"key":"EntryName","value":"EFG_III"}]},{"database":"InterPro","id":"IPR035647","properties":[{"key":"EntryName","value":"EFG_III/V"}]},{"database":"InterPro","id":"IPR047872","properties":[{"key":"EntryName","value":"EFG_IV"}]},{"database":"InterPro","id":"IPR035649","properties":[{"key":"EntryName","value":"EFG_V"}]},{"database":"InterPro","id":"IPR000640","properties":[{"key":"EntryName","value":"EFG_V-like"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR005225","properties":[{"key":"EntryName","value":"Small_GTP-bd"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR004540","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2"}]},{"database":"InterPro","id":"IPR005517","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2_IV"}]},{"database":"NCBIfam","id":"TIGR00484","properties":[{"key":"EntryName","value":"EF-G"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009381","properties":[{"key":"EntryName","value":"PRK12740.1-5"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00231","properties":[{"key":"EntryName","value":"small_GTP"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261:SF1","properties":[{"key":"EntryName","value":"RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261","properties":[{"key":"EntryName","value":"TRANSLATION ELONGATION FACTOR G-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22042","properties":[{"key":"EntryName","value":"EF-G_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00679","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14492","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03764","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"SMART","id":"SM00838","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00889","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54980","properties":[{"key":"EntryName","value":"EF-G C-terminal domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSKINKLEHIRNIGICAHIDAGKTTTTERILYYTGKSHKIGEVHEGGATMDWMEQEQERGITITSAATTCRWQDKIINIIDTPGHVDFTIEVERSLRVLDGAVAVFDGVAGVEPQSETVWRQADKYNVPRMCFVNKMDRMGADFYRCVEMLKDRLGAKPLVIQLPVGIEENFKGIIDLIKMKAVIWKDEALGAEYFEEDIPADMKDKAEEYRAKLLDMVVELDDHVMEKYLSGEEVTAEEIKRLIRKGTISAAFYPVLCGSAFKNKGVQPLLDAVVDFLPSPIDIGIVKGMEVSTGEEKDFPISVTEPFAALRFKIMNDPFVGSLTFIRIYSGKITSGTTVINTVKNKREKIGRMLLMHANNREDVKEASAGDIVALAGLKDTTTGDTLSDIDQQVILERMEFPEPVIELAVEPKSTADQEKMGLALSRLAAEDPSFRVSTDYETGQTVIKGMGELHLEIIIDRMRREFKVEANIGAPQVAYRETITKVCEIDYTHKKQSGGAGQFARVKIIFEPLKEVKDLKDEDKNKNFVFESKIIGGAVPKEYIPGVEKGLNNIRETGVIAGYPMIDFKATLVDGAFHDVDSSVLAFEIAAKAAFREGMPKGNPKLLEPIMQVEVITPDEYMGDIIGDLNSRRGQIQSMDPRGNAQVVTANVPLAEMFGYVNTLRSLSQGRAQFSMIFSHYDQVPSQVADIIKAKK","length":699,"molWeight":77794,"crc64":"FBFA71C26014A358","md5":"2573BFD3617D999DEF344AF00DA97018"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Binding site":3},"uniParcId":"UPI00000B7ADA"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O33544","uniProtkbId":"FMT_RICRI","entryAudit":{"firstPublicDate":"2000-05-30","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-01-01","entryVersion":74,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P23882"}],"value":"Methionyl-tRNA formyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P23882"}],"value":"2.1.2.9"}]},"flag":"Fragment"},"genes":[{"geneName":{"value":"fmt"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P23882"}],"value":"Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-methionyl-tRNA(fMet) + (6R)-10-formyltetrahydrofolate = N-formyl-L-methionyl-tRNA(fMet) + (6S)-5,6,7,8-tetrahydrofolate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24380"},{"database":"Rhea","id":"RHEA-COMP:9952"},{"database":"Rhea","id":"RHEA-COMP:9953"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57453"},{"database":"ChEBI","id":"CHEBI:78530"},{"database":"ChEBI","id":"CHEBI:78844"},{"database":"ChEBI","id":"CHEBI:195366"}],"ecNumber":"2.1.2.9","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P23882"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Fmt family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"OUTSIDE"},"end":{"value":73,"modifier":"EXACT"}},"description":"Methionyl-tRNA formyltransferase","featureId":"PRO_0000083035"},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":""}],"keywords":[{"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-19P7K656VHGCA","citationType":"submission","authors":["Andersson S.G.E.","Stothard D.R.","Romedenne M.","Viseur N.","Fuerst P.","Kurland C.G."],"title":"Rearrangement of the rRNA genes in Rickettsia preceeded the divergence of the typhus and the spotted fever group Rickettsia.","publicationDate":"MAY-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Y13125","properties":[{"key":"ProteinId","value":"CAA73592.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"O33544","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O33544","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0004479","properties":[{"key":"GoTerm","value":"F:methionyl-tRNA formyltransferase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"CDD","id":"cd08704","properties":[{"key":"EntryName","value":"Met_tRNA_FMT_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.10.25.10","properties":[{"key":"EntryName","value":"Formyl transferase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005793","properties":[{"key":"EntryName","value":"Formyl_trans_C"}]},{"database":"InterPro","id":"IPR037022","properties":[{"key":"EntryName","value":"Formyl_trans_C_sf"}]},{"database":"InterPro","id":"IPR011034","properties":[{"key":"EntryName","value":"Formyl_transferase-like_C_sf"}]},{"database":"InterPro","id":"IPR044135","properties":[{"key":"EntryName","value":"Met-tRNA-FMT_C"}]},{"database":"Pfam","id":"PF02911","properties":[{"key":"EntryName","value":"Formyl_trans_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50486","properties":[{"key":"EntryName","value":"FMT C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"YFSYNDKIIKILEAEYLNADHHFTSGTVISDKLEIACGSGILRVKKLQQESKKALSIEEFLRGTNILKDTVLK","length":73,"molWeight":8287,"crc64":"7490F34A8B48EB64","md5":"CC005F4115F1B3BDCC59C453F8CA5DF2"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Non-terminal residue":1},"uniParcId":"UPI000012AB04"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8KTC0","uniProtkbId":"SECE_RICRI","entryAudit":{"firstPublicDate":"2007-01-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-10-01","entryVersion":70,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00422"}],"value":"Protein translocase subunit SecE"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00422"}],"value":"secE"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00422"}],"value":"Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00422"}],"value":"Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00422"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00422"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00422"}],"value":"Belongs to the SecE/SEC61-gamma family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Protein translocase subunit SecE","featureId":"PRO_0000273133"},{"type":"Transmembrane","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00422"}]}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-0811","category":"Biological process","name":"Translocation"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"12140235","citationType":"journal article","authors":["Amiri H.","Alsmark C.M.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"12140235"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a004184"}],"title":"Proliferation and deterioration of Rickettsia palindromic elements.","publicationDate":"2002","journal":"Mol. Biol. Evol.","firstPage":"1234","lastPage":"1243","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"Sawtooth","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF502170","properties":[{"key":"ProteinId","value":"AAM90914.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012150392.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"Q8KTC0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8KTC0","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937974","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"DIREVWM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008320","properties":[{"key":"GoTerm","value":"F:transmembrane protein transporter activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0065002","properties":[{"key":"GoTerm","value":"P:intracellular protein transmembrane transport"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009306","properties":[{"key":"GoTerm","value":"P:protein secretion"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006605","properties":[{"key":"GoTerm","value":"P:protein targeting"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0043952","properties":[{"key":"GoTerm","value":"P:protein transport by the Sec complex"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"1.20.5.1030","properties":[{"key":"EntryName","value":"Preprotein translocase secy subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00422","properties":[{"key":"EntryName","value":"SecE"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005807","properties":[{"key":"EntryName","value":"SecE_bac"}]},{"database":"InterPro","id":"IPR038379","properties":[{"key":"EntryName","value":"SecE_sf"}]},{"database":"InterPro","id":"IPR001901","properties":[{"key":"EntryName","value":"Translocase_SecE/Sec61-g"}]},{"database":"NCBIfam","id":"TIGR00964","properties":[{"key":"EntryName","value":"secE_bact"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33910","properties":[{"key":"EntryName","value":"PROTEIN TRANSLOCASE SUBUNIT SECE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33910:SF1","properties":[{"key":"EntryName","value":"PROTEIN TRANSLOCASE SUBUNIT SECE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00584","properties":[{"key":"EntryName","value":"SecE"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01067","properties":[{"key":"EntryName","value":"SECE_SEC61G"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFKEYKIYKFFEQVKQETYKVVWPTRKELVASTLVVVVAVFIFSLTCLVLDYSIHNIMQLLLNIGK","length":66,"molWeight":7817,"crc64":"6175DE68690E57BC","md5":"7E521086CA589793EA87817536CF75E7"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":1},"uniParcId":"UPI00000AF87C"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9AKH1","uniProtkbId":"FER2_RICRI","entryAudit":{"firstPublicDate":"2005-12-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":84,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"2Fe-2S ferredoxin"}},"alternativeNames":[{"fullName":{"value":"Adrenodoxin-like protein"}}]},"genes":[{"geneName":{"value":"fdxB"},"synonyms":[{"value":"adx1"}]}],"comments":[{"texts":[{"value":"Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"[2Fe-2S] cluster","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:190135"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 1 [2Fe-2S] cluster."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the adrenodoxin/putidaredoxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"2Fe-2S ferredoxin","featureId":"PRO_0000201178"},{"type":"Domain","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"2Fe-2S ferredoxin-type","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00465"}]},{"type":"Binding site","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00465"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}},{"type":"Binding site","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00465"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}},{"type":"Binding site","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00465"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}},{"type":"Binding site","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00465"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}}],"keywords":[{"id":"KW-0001","category":"Ligand","name":"2Fe-2S"},{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"11319266","citationType":"journal article","authors":["Andersson J.O.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"11319266"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a003864"}],"title":"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes.","publicationDate":"2001","journal":"Mol. Biol. Evol.","firstPage":"829","lastPage":"839","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"84-21C","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ293320","properties":[{"key":"ProteinId","value":"CAC33690.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012150478.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"Q9AKH1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9AKH1","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937045","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"TCHCIIR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0051537","properties":[{"key":"GoTerm","value":"F:2 iron, 2 sulfur cluster binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0140647","properties":[{"key":"GoTerm","value":"P:P450-containing electron transport chain"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00207","properties":[{"key":"EntryName","value":"fer2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.10.20.30","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036010","properties":[{"key":"EntryName","value":"2Fe-2S_ferredoxin-like_sf"}]},{"database":"InterPro","id":"IPR001041","properties":[{"key":"EntryName","value":"2Fe-2S_ferredoxin-type"}]},{"database":"InterPro","id":"IPR001055","properties":[{"key":"EntryName","value":"Adrenodoxin-like"}]},{"database":"InterPro","id":"IPR018298","properties":[{"key":"EntryName","value":"Adrenodoxin_Fe-S_BS"}]},{"database":"InterPro","id":"IPR012675","properties":[{"key":"EntryName","value":"Beta-grasp_dom_sf"}]},{"database":"PANTHER","id":"PTHR23426:SF72","properties":[{"key":"EntryName","value":"2FE-2S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR23426","properties":[{"key":"EntryName","value":"FERREDOXIN/ADRENODOXIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00111","properties":[{"key":"EntryName","value":"Fer2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00355","properties":[{"key":"EntryName","value":"ADRENODOXIN"}]},{"database":"SUPFAM","id":"SSF54292","properties":[{"key":"EntryName","value":"2Fe-2S ferredoxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51085","properties":[{"key":"EntryName","value":"2FE2S_FER_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00814","properties":[{"key":"EntryName","value":"ADX"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSGKIKVTFIINDGEEKTVEAPIGLSILEIAHSNDLDLEGACEGSLACATCHVILEEEFYNKLKKPTEAEEDMLDLAFGLTDTSRLGCQIILTEELDGIKVHLPAATRNIKL","length":112,"molWeight":12215,"crc64":"4E180C0063FB742C","md5":"578A8FA1C0052B57240F789C1BFF78AE"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Binding site":4},"uniParcId":"UPI00000B243F"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9AKJ0","uniProtkbId":"RICKA_RICRI","entryAudit":{"firstPublicDate":"2006-10-31","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":56,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Arp2/3 complex-activating protein rickA"}},"alternativeNames":[{"fullName":{"value":"Actin polymerization protein rickA"}}]},"genes":[{"geneName":{"value":"rickA"},"orfNames":[{"value":"ORFB4"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15236643"}],"value":"Recruits and activates the Arp2/3 complex, which in turn leads to actin polymerization, promoting Rickettsia motility during infection"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cell surface","id":"SL-0310"}}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":494,"modifier":"EXACT"}},"description":"Arp2/3 complex-activating protein rickA","featureId":"PRO_0000259653"},{"type":"Domain","location":{"start":{"value":383,"modifier":"EXACT"},"end":{"value":400,"modifier":"EXACT"}},"description":"WH2"},{"type":"Region","location":{"start":{"value":312,"modifier":"EXACT"},"end":{"value":494,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":421,"modifier":"EXACT"},"end":{"value":454,"modifier":"EXACT"}},"description":"Central and acidic domains"},{"type":"Compositional bias","location":{"start":{"value":317,"modifier":"EXACT"},"end":{"value":357,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":440,"modifier":"EXACT"},"end":{"value":456,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":477,"modifier":"EXACT"},"end":{"value":494,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0009","category":"Molecular function","name":"Actin-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"11319266","citationType":"journal article","authors":["Andersson J.O.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"11319266"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a003864"}],"title":"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes.","publicationDate":"2001","journal":"Mol. Biol. Evol.","firstPage":"829","lastPage":"839","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"84-21C","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"15236643","citationType":"journal article","authors":["Jeng R.L.","Goley E.D.","D'Alessio J.A.","Chaga O.Y.","Svitkina T.M.","Borisy G.G.","Heinzen R.A.","Welch M.D."],"citationCrossReferences":[{"database":"PubMed","id":"15236643"},{"database":"DOI","id":"10.1111/j.1462-5822.2004.00402.x"}],"title":"A Rickettsia WASP-like protein activates the Arp2/3 complex and mediates actin-based motility.","publicationDate":"2004","journal":"Cell. Microbiol.","firstPage":"761","lastPage":"769","volume":"6"},"referencePositions":["FUNCTION"],"referenceComments":[{"value":"HLP","type":"STRAIN"},{"value":"Norgaard","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ293314","properties":[{"key":"ProteinId","value":"CAC33668.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q9AKJ0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9AKJ0","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009986","properties":[{"key":"GoTerm","value":"C:cell surface"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"Gene3D","id":"6.10.280.150","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR051661","properties":[{"key":"EntryName","value":"Actin_filament_regulator"}]},{"database":"PANTHER","id":"PTHR47102","properties":[{"key":"EntryName","value":"PROTEIN BNI1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47102:SF2","properties":[{"key":"EntryName","value":"PROTEIN BNI1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVKEIDINKLLAQENNALNAILSHVNELCKQNKQLQGLIEIQNETKELEKEHNRSLPWFKRFVKTVSNVKYILIKSEEQLTNEAIKYNNKILKDIDNKIYNIAEKSAPLKQALQEEIEKSFKDLTKKDLSKDQRARLSEVFFSYKSKPERFSALHMTNPLQFINAEALEKQFNSLNATKQNIQNLISENSNIKELKEIQKQVAEIRAEVPHTFFEKLNNIWQNVKNVFVNNSEQVLAKNKESNTRTIRKIDEQLYKTQHKFEELIENKERNIKDIIAKLPDNEELQKIVSNLTNHMASKKEPILANVSLAKPLENNIPPPPPPPPPLPDNNIPPPPPPPPPLPDNNIPPPPPPPPMAPVKTLSKAVEATTVKKLENQPRPSIDTSDLMREIAGPKKLKKVEFDPNTGKPVAHSHSKPAQNVNKPSGLESIFARRVAIEMSDSSSSESDSGNWSDVSVNRNKSKMLKTKGERDAKMTTHAQKINNRNSQKPSFVR","length":494,"molWeight":56190,"crc64":"4334E4E8307A063D","md5":"0B3F507E645B1D25978B871113B2D5A7"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1},"countByFeatureType":{"Chain":1,"Domain":1,"Region":2,"Compositional bias":3},"uniParcId":"UPI00000B9337"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P32204","uniProtkbId":"FABZ_RICRI","entryAudit":{"firstPublicDate":"1993-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1993-10-01","entryVersion":93,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}],"value":"3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}],"value":"4.2.1.59"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}],"value":"(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}],"value":"(3R)-hydroxymyristoyl-ACP dehydrase"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}],"value":"Beta-hydroxyacyl-ACP dehydratase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}],"value":"fabZ"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}],"value":"Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13097"},{"database":"Rhea","id":"RHEA-COMP:9925"},{"database":"Rhea","id":"RHEA-COMP:9945"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78784"},{"database":"ChEBI","id":"CHEBI:78827"}],"ecNumber":"4.2.1.59","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}],"value":"Belongs to the thioester dehydratase family. FabZ subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ","featureId":"PRO_0000091722"},{"type":"Active site","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00406"}]}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0441","category":"Biological process","name":"Lipid A biosynthesis"},{"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"}],"references":[{"referenceNumber":1,"citation":{"id":"8125326","citationType":"journal article","authors":["Shaw E.I.","Wood D.O."],"citationCrossReferences":[{"database":"PubMed","id":"8125326"},{"database":"DOI","id":"10.1016/0378-1119(94)90739-0"}],"title":"Characterization of a Rickettsia rickettsii DNA fragment analogous to the fir A-ORF17-lpxA region of Escherichia coli.","publicationDate":"1994","journal":"Gene","firstPage":"109","lastPage":"113","volume":"140"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L22690","properties":[{"key":"ProteinId","value":"AAA26385.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_004997000.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"P32204","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P32204","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79936821","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"95361756","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FPGRPLM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0019171","properties":[{"key":"GoTerm","value":"F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006633","properties":[{"key":"GoTerm","value":"P:fatty acid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009245","properties":[{"key":"GoTerm","value":"P:lipid A biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd01288","properties":[{"key":"EntryName","value":"FabZ"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.10.129.10:FF:000001","properties":[{"key":"EntryName","value":"3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.10.129.10","properties":[{"key":"EntryName","value":"Hotdog Thioesterase"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00406","properties":[{"key":"EntryName","value":"FabZ"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013114","properties":[{"key":"EntryName","value":"FabA_FabZ"}]},{"database":"InterPro","id":"IPR010084","properties":[{"key":"EntryName","value":"FabZ"}]},{"database":"InterPro","id":"IPR029069","properties":[{"key":"EntryName","value":"HotDog_dom_sf"}]},{"database":"NCBIfam","id":"TIGR01750","properties":[{"key":"EntryName","value":"fabZ"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF000582","properties":[{"key":"EntryName","value":"PRK00006.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30272","properties":[{"key":"EntryName","value":"3-HYDROXYACYL-[ACYL-CARRIER-PROTEIN] DEHYDRATASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30272:SF1","properties":[{"key":"EntryName","value":"3-HYDROXYACYL-[ACYL-CARRIER-PROTEIN] DEHYDRATASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07977","properties":[{"key":"EntryName","value":"FabA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54637","properties":[{"key":"EntryName","value":"Thioesterase/thiol ester dehydrase-isomerase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIIDITEIMDWIPHRYPFLLVDRVLKIDPNKSITGIKNVTVNEPQFTGHFPARPVMPGVLMVEAMAQLAAILVAKSLGSTKNKEVFLMTIENAKFRRIVQPGDTMHIHAVIDQQRANVWKFSSTVTVEGEIAAESKFTAMIKDKT","length":145,"molWeight":16290,"crc64":"D4F5D14B13B9A456","md5":"9FD45C13C778B77EBF5F517CCA613ACD"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1},"uniParcId":"UPI000012A4B7"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9AKF3","uniProtkbId":"HLP_RICRI","entryAudit":{"firstPublicDate":"2007-03-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":73,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Histone-like DNA-binding protein"}}},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the bacterial histone-like protein family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"Histone-like DNA-binding protein","featureId":"PRO_0000280389"}],"keywords":[{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"11319266","citationType":"journal article","authors":["Andersson J.O.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"11319266"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a003864"}],"title":"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes.","publicationDate":"2001","journal":"Mol. Biol. Evol.","firstPage":"829","lastPage":"839","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"84-21C","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ293329","properties":[{"key":"ProteinId","value":"CAC33712.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012151223.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"Q9AKF3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9AKF3","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937736","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AIVHTVF","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0030527","properties":[{"key":"GoTerm","value":"F:structural constituent of chromatin"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"4.10.520.10","properties":[{"key":"EntryName","value":"IHF-like DNA-binding proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR000119","properties":[{"key":"EntryName","value":"Hist_DNA-bd"}]},{"database":"InterPro","id":"IPR010992","properties":[{"key":"EntryName","value":"IHF-like_DNA-bd_dom_sf"}]},{"database":"PANTHER","id":"PTHR33175","properties":[{"key":"EntryName","value":"DNA-BINDING PROTEIN HU"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33175:SF2","properties":[{"key":"EntryName","value":"INTEGRATION HOST FACTOR SUBUNIT ALPHA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00216","properties":[{"key":"EntryName","value":"Bac_DNA_binding"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00411","properties":[{"key":"EntryName","value":"BHL"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF47729","properties":[{"key":"EntryName","value":"IHF-like DNA-binding proteins"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTITKAKIAAMLSSKLGFSNHLCEEIVHTVFSNILEIAKAQKLTLKNFGSFEVKQKNPRPGINFHTKALVIIESKKHLRFVPSSKLKALINESTR","length":95,"molWeight":10662,"crc64":"8DB404CB7D1ADB23","md5":"D6337CE3791BEE256FA28F896720E6DE"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI00000BD685"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9AKI4","uniProtkbId":"Y600_RICRI","entryAudit":{"firstPublicDate":"2002-01-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":52,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"4: Predicted","proteinDescription":{"recommendedName":{"fullName":{"value":"Uncharacterized protein RP600 homolog"}}},"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"Uncharacterized protein RP600 homolog","featureId":"PRO_0000101402"},{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"Rhodanese","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00173"}]}],"references":[{"referenceNumber":1,"citation":{"id":"11319266","citationType":"journal article","authors":["Andersson J.O.","Andersson S.G.E."],"citationCrossReferences":[{"database":"PubMed","id":"11319266"},{"database":"DOI","id":"10.1093/oxfordjournals.molbev.a003864"}],"title":"Pseudogenes, junk DNA, and the dynamics of Rickettsia genomes.","publicationDate":"2001","journal":"Mol. Biol. Evol.","firstPage":"829","lastPage":"839","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"84-21C","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ293314","properties":[{"key":"ProteinId","value":"CAC33674.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_014362486.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"Q9AKI4","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9AKI4","properties":[{"key":"Description","value":"-"}]},{"database":"CDD","id":"cd01522","properties":[{"key":"EntryName","value":"RHOD_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.250.10","properties":[{"key":"EntryName","value":"Rhodanese-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001763","properties":[{"key":"EntryName","value":"Rhodanese-like_dom"}]},{"database":"InterPro","id":"IPR036873","properties":[{"key":"EntryName","value":"Rhodanese-like_dom_sf"}]},{"database":"InterPro","id":"IPR044240","properties":[{"key":"EntryName","value":"STR4-like"}]},{"database":"InterPro","id":"IPR064055","properties":[{"key":"EntryName","value":"STR4_M"}]},{"database":"PANTHER","id":"PTHR47377","properties":[{"key":"EntryName","value":"RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 4, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47377:SF1","properties":[{"key":"EntryName","value":"RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 4, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF30980","properties":[{"key":"EntryName","value":"STR4_M"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00450","properties":[{"key":"EntryName","value":"RHOD"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52821","properties":[{"key":"EntryName","value":"Rhodanese/Cell cycle control phosphatase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50206","properties":[{"key":"EntryName","value":"RHODANESE_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSVQNICSTKAYDMLISNDNAFLVDVRTREEWQQVGIPHLDNKNKMLFLSWQLNKDFEDNFLSIINDKIHAIIFFLCRSGYRSFIAANFITNIGYKNCYNISDGFEGNNQDKGWKQNNLPWQF","length":123,"molWeight":14454,"crc64":"101ECE50CF98F033","md5":"422D3ED1CD5E35DCE07FC0AFD402D75B"},"extraAttributes":{"countByFeatureType":{"Chain":1,"Domain":1},"uniParcId":"UPI0000139B13"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q7X081","uniProtkbId":"Q7X081_RICRI","entryAudit":{"firstPublicDate":"2003-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-10-01","entryVersion":126,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP79313.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003874"}],"value":"Protein translocase subunit SecA"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"value":"7.4.2.8"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP79313.1"}],"value":"secA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"value":"Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP + H2O + cellular proteinSide 1 = ADP + phosphate + cellular proteinSide 2.","ecNumber":"7.4.2.8","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001947"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"value":"Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"value":"Distribution is 50-50"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"value":"Peripheral membrane protein","id":"SL-9903"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"value":"Cytoplasmic side","id":"SL-9910"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007650"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003874"}],"value":"Belongs to the SecA family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":608,"modifier":"EXACT"}},"description":"SecA family profile","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51196"}]},{"type":"Domain","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":246,"modifier":"EXACT"}},"description":"Helicase ATP-binding","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51192"}]},{"type":"Region","location":{"start":{"value":863,"modifier":"EXACT"},"end":{"value":887,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":499,"modifier":"EXACT"},"end":{"value":499,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022519"}],"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022475"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00541"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022927"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023010"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"id":"KW-0811","category":"Biological process","name":"Translocation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01382"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022833"}],"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"15699207","citationType":"journal article","authors":["Rahman M.S.","Simser J.A.","Macaluso K.R.","Azad A.F."],"citationCrossReferences":[{"database":"PubMed","id":"15699207"},{"database":"DOI","id":"10.1099/mic.0.27556-0"}],"title":"Functional analysis of secA homologues from rickettsiae.","publicationDate":"2005","journal":"Microbiology","firstPage":"589","lastPage":"596","volume":"151"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP79313.1"}],"value":"Sheila Smith","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP79313.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY312572","properties":[{"key":"ProteinId","value":"AAP79313.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012151042.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP018913.1"}]},{"database":"AlphaFoldDB","id":"Q7X081","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q7X081","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"79937562","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"MVHYDVQ","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0031522","properties":[{"key":"GoTerm","value":"C:cell envelope Sec protein transport complex"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008564","properties":[{"key":"GoTerm","value":"F:protein-exporting ATPase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0065002","properties":[{"key":"GoTerm","value":"P:intracellular protein transmembrane transport"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0017038","properties":[{"key":"GoTerm","value":"P:protein import"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006605","properties":[{"key":"GoTerm","value":"P:protein targeting"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0043952","properties":[{"key":"GoTerm","value":"P:protein transport by the Sec complex"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"CDD","id":"cd17928","properties":[{"key":"EntryName","value":"DEXDc_SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd18803","properties":[{"key":"EntryName","value":"SF2_C_secA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000113","properties":[{"key":"EntryName","value":"Preprotein translocase subunit SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.1440.10:FF:000001","properties":[{"key":"EntryName","value":"Preprotein translocase subunit SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.3060.10:FF:000003","properties":[{"key":"EntryName","value":"Protein translocase subunit SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000334","properties":[{"key":"EntryName","value":"Protein translocase subunit SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.3060.10","properties":[{"key":"EntryName","value":"Helical scaffold and wing domains of SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.90.1440.10","properties":[{"key":"EntryName","value":"SecA, preprotein cross-linking domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01382","properties":[{"key":"EntryName","value":"SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR014001","properties":[{"key":"EntryName","value":"Helicase_ATP-bd"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR004027","properties":[{"key":"EntryName","value":"SEC_C_motif"}]},{"database":"InterPro","id":"IPR000185","properties":[{"key":"EntryName","value":"SecA"}]},{"database":"InterPro","id":"IPR020937","properties":[{"key":"EntryName","value":"SecA_CS"}]},{"database":"InterPro","id":"IPR011115","properties":[{"key":"EntryName","value":"SecA_DEAD"}]},{"database":"InterPro","id":"IPR014018","properties":[{"key":"EntryName","value":"SecA_motor_DEAD"}]},{"database":"InterPro","id":"IPR011130","properties":[{"key":"EntryName","value":"SecA_preprotein_X-link_dom"}]},{"database":"InterPro","id":"IPR044722","properties":[{"key":"EntryName","value":"SecA_SF2_C"}]},{"database":"InterPro","id":"IPR011116","properties":[{"key":"EntryName","value":"SecA_Wing/Scaffold"}]},{"database":"InterPro","id":"IPR036266","properties":[{"key":"EntryName","value":"SecA_Wing/Scaffold_sf"}]},{"database":"InterPro","id":"IPR036670","properties":[{"key":"EntryName","value":"SecA_X-link_sf"}]},{"database":"NCBIfam","id":"NF009538","properties":[{"key":"EntryName","value":"PRK12904.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00963","properties":[{"key":"EntryName","value":"secA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30612:SF0","properties":[{"key":"EntryName","value":"CHLOROPLAST PROTEIN-TRANSPORTING ATPASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30612","properties":[{"key":"EntryName","value":"SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21090","properties":[{"key":"EntryName","value":"P-loop_SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02810","properties":[{"key":"EntryName","value":"SEC-C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07517","properties":[{"key":"EntryName","value":"SecA_DEAD"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01043","properties":[{"key":"EntryName","value":"SecA_PP_bind"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07516","properties":[{"key":"EntryName","value":"SecA_SW"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00906","properties":[{"key":"EntryName","value":"SECA"}]},{"database":"SMART","id":"SM00957","properties":[{"key":"EntryName","value":"SecA_DEAD"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00958","properties":[{"key":"EntryName","value":"SecA_PP_bind"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81886","properties":[{"key":"EntryName","value":"Helical scaffold and wing domains of SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF81767","properties":[{"key":"EntryName","value":"Pre-protein crosslinking domain of SecA"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51192","properties":[{"key":"EntryName","value":"HELICASE_ATP_BIND_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01312","properties":[{"key":"EntryName","value":"SECA"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51196","properties":[{"key":"EntryName","value":"SECA_MOTOR_DEAD"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLSILKKLFGTANDRTVKKLFSEITKINSLEPAIQKLSDEELKNKTVEFKEKLKNGATLDDIVYEAFAVVREAAKRVCGMRHFDVQLIGGLILHRGMITEMRTGEGKTLVATLPAYLNALTGKGVHVVTVNDYLARRDSAAMGKIYNFLGLSVGCIVGGMPDEVKRAAYNADITHATNNELGFDYLRDNMKYSLQERVLRPFNFAIIDEVDSILIDEARTPLVISGPVNDNAELYGKIDKIVRLLNASDFEKDEKLKTINLTETGITHIESLLSKENIIKPDTSLYDFENLTLVHYINQALRAHNMFTVNVDYLVREGKVMIIDEFTGRVMEGRRYSEGLHQALEAKENVKIQNENQTLASITFQNYFRNYPKLSGMTGTAMTEAPELKDIYNLDVVAVPTHNKVTRLDLDDEIYGSKKEKYDAILKLIRDCYDRGQPILVGTISIEKSEELSSVLNKENIPHKVLNAKFHEQEAFIIAQAGRFKAVTIATNMAGRGTDIMLGGNPEMLIEQLDKEHNYEAKIAEIKAQIAEEKKQVIEAGGLFVIGTERHESRRIDNQLRGRSGRQGDPGKTKFFLSLDDDLMRIFASDRISGVLRTLGLKDGEAIHHPMISRSLEKAQQKVEGHNYEMRKNLLRFDDVMNDQRKIIYEQRTEIIKSKDSHGFLNSTTEELAKKIVLTFMPVGSYREDWDIENLSVELHRVFSIKFDHNVVSKNDVTEEEITKTVIQMAHDIYKSKEEAYSSELMHNAVKYILLTTLDQVWKDHLYSLDHLRQGISLRAYGQKDPLSEYKREAFNLFEQMLNNLKELFIQTVYHFHIDLKNVQKEDVSLEYKKLQKNMCESREDPAFSKYNAGSSLETDLKPVVSRIDPKDRNPDDPTSWGRVSRNELCPCGSGKKYKYCHGANE","length":906,"molWeight":103264,"crc64":"AFE6DA392ACEF98A","md5":"F3DCA41B2AC52B7E3C95B90721795537"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Region":1,"Binding site":3},"uniParcId":"UPI00001AE369"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1P8NQI7","uniProtkbId":"A0A1P8NQI7_RICRI","entryAudit":{"firstPublicDate":"2017-04-12","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2017-04-12","entryVersion":20,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"APX42719.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}],"value":"Citrate (Si)-synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}],"value":"2.3.3.1"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"APX42719.1"}],"value":"gltA"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16845"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16452"},{"database":"ChEBI","id":"CHEBI:16947"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"}],"ecNumber":"2.3.3.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004751"}],"value":"Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010566"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003406"}],"value":"Belongs to the citrate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Active site","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"APX42719.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"APX42719.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003406"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022532"}],"id":"KW-0816","category":"Biological process","name":"Tricarboxylic acid cycle"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-6GNFVMV93DE8B","citationType":"submission","authors":["Emmerick S.D.","Toma H.K.","Machado C.S.","Junior E.S.","Cordeiro M.D.","Telleria E.L.","Cunha N.C.","Almosny N.P."],"publicationDate":"JUL-2016","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"APX42719.1"}],"value":"RC83","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"APX42719.1"}]},{"referenceNumber":2,"citation":{"id":"32267390","citationType":"journal article","authors":["Campos S.D.E.","Cunha N.C.D.","Machado C.S.C.","Nadal N.V.","Seabra Junior E.S.","Telleria E.L.","Cordeiro M.D.","Toma H.K.","Almosny N.R.P."],"citationCrossReferences":[{"database":"PubMed","id":"32267390"}],"title":"Spotted fever group rickettsial infection in dogs and their ticks from domestic-wildlife interface areas in southeastern Brazil.","publicationDate":"2020","journal":"Rev. Bras. Parasitol. Vet.","firstPage":"0","lastPage":"e020219","volume":"29"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"APX42719.1"}],"value":"RC83","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"APX42719.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KX619438","properties":[{"key":"ProteinId","value":"APX42719.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1P8NQI7","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00223","properties":[{"key":"RectionId","value":"UER00717"}]},{"database":"GO","id":"GO:0036440","properties":[{"key":"GoTerm","value":"F:citrate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006099","properties":[{"key":"GoTerm","value":"P:tricarboxylic acid cycle"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"FunFam","id":"1.10.230.10:FF:000002","properties":[{"key":"EntryName","value":"Citrate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.230.10","properties":[{"key":"EntryName","value":"Cytochrome P450-Terp, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR016143","properties":[{"key":"EntryName","value":"Citrate_synth-like_sm_a-sub"}]},{"database":"InterPro","id":"IPR002020","properties":[{"key":"EntryName","value":"Citrate_synthase"}]},{"database":"InterPro","id":"IPR019810","properties":[{"key":"EntryName","value":"Citrate_synthase_AS"}]},{"database":"InterPro","id":"IPR036969","properties":[{"key":"EntryName","value":"Citrate_synthase_sf"}]},{"database":"PANTHER","id":"PTHR42871","properties":[{"key":"EntryName","value":"CITRATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42871:SF1","properties":[{"key":"EntryName","value":"CITRATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00285","properties":[{"key":"EntryName","value":"Citrate_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00143","properties":[{"key":"EntryName","value":"CITRTSNTHASE"}]},{"database":"SUPFAM","id":"SSF48256","properties":[{"key":"EntryName","value":"Citrate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00480","properties":[{"key":"EntryName","value":"CITRATE_SYNTHASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WGPAHGGANEAVINMLKEIGSSEYIPKYIAKAKDKNDPFRLMGFGHRVYKNYDPRAVVLKETCKEVLKELGQLDNNPLLQIAIELEAIALKDEYFIERKLYPNVDFYSGIIYKAMGIPS","length":119,"molWeight":13507,"crc64":"DAE9917DDD797BDB","md5":"4D031C380F8BFD2009870D3FB0E7C8DF"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI000973B69B"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A4Y6GM69","uniProtkbId":"A0A4Y6GM69_RICRI","entryAudit":{"firstPublicDate":"2019-09-18","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2019-09-18","entryVersion":19,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QDF44366.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00015281"}],"value":"17 kDa surface antigen"}},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QDF44366.1"}],"value":"htrA"}}],"comments":[{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004459"}],"value":"Cell outer membrane","id":"SL-0040"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004459"}],"value":"Lipid-anchor","id":"SL-9901"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008681"}],"value":"Belongs to the rickettsiale 17 kDa surface antigen family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"17 kDa surface antigen","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5021462840"},{"type":"Domain","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"Glycine zipper 2TM","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF05433"}]},{"type":"Domain","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"Surface antigen","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF16998"}]},{"type":"Lipidation","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"N-palmitoyl cysteine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Lipidation","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"S-diacylglycerol cysteine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Non-terminal residue","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QDF44366.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023237"}],"id":"KW-0998","category":"Cellular component","name":"Cell outer membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023288"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}],"id":"KW-0449","category":"PTM","name":"Lipoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023139"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}],"id":"KW-0564","category":"PTM","name":"Palmitate"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}],"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"31201126","citationType":"journal article","authors":["Guzman-Cornejo C.","Sanchez-Montes S.","Caso A.","Rendon-Franco E.","Munoz-Garcia C.I."],"citationCrossReferences":[{"database":"PubMed","id":"31201126"}],"title":"Molecular detection of Rickettsia rickettsii in ticks associated with the bobcat (Lynx rufus) in northeast Mexico.","publicationDate":"2019","journal":"Ticks Tick Borne Dis.","firstPage":"1105","lastPage":"1108","volume":"10"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QDF44366.1"}],"value":"RRDVTamMex","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QDF44366.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"MK744159","properties":[{"key":"ProteinId","value":"QDF44366.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A4Y6GM69","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009279","properties":[{"key":"GoTerm","value":"C:cell outer membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"InterPro","id":"IPR032635","properties":[{"key":"EntryName","value":"Anti_2"}]},{"database":"InterPro","id":"IPR008816","properties":[{"key":"EntryName","value":"Gly_zipper_2TM_dom"}]},{"database":"InterPro","id":"IPR016364","properties":[{"key":"EntryName","value":"Surface_antigen_Rickettsia"}]},{"database":"Pfam","id":"PF16998","properties":[{"key":"EntryName","value":"17kDa_Anti_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05433","properties":[{"key":"EntryName","value":"Rick_17kDa_Anti"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF002721","properties":[{"key":"EntryName","value":"Surface_antigen_Rickettsia"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51257","properties":[{"key":"EntryName","value":"PROKAR_LIPOPROTEIN"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKLLSKIMIIALATSMLQACNGPGGMNKQGTGTLLGGAGGALLGSQFGKGKGQLVGVGVGALLGAVLGGQIGAGMDEQDRRLAELTSQRALETAPSGSNVEWRNPDNGNYGYVTPNKTYRNSTGQYCREYTQTVVIGGKQQKAYGNACRQPDGQW","length":155,"molWeight":16140,"crc64":"E4FCE16976F17E7E","md5":"778E61214A2D3D4B037B57AB246ABA76"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":2,"Lipidation":2,"Non-terminal residue":1},"uniParcId":"UPI001163B56B"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A6M3QJ09","uniProtkbId":"A0A6M3QJ09_RICRI","entryAudit":{"firstPublicDate":"2020-10-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2020-10-07","entryVersion":16,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QJC19243.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022134"},{"evidenceCode":"ECO:0000256","source":"NCBIfam","id":"TIGR01798"}],"value":"Citrate synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012972"},{"evidenceCode":"ECO:0000256","source":"NCBIfam","id":"TIGR01798"}],"value":"2.3.3.16"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QJC19243.1"}],"value":"gltA"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16845"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16452"},{"database":"ChEBI","id":"CHEBI:16947"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"}],"ecNumber":"2.3.3.16","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003370"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16845"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16452"},{"database":"ChEBI","id":"CHEBI:16947"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"}],"ecNumber":"2.3.3.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004751"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003370"}],"value":"Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010566"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003406"}],"value":"Belongs to the citrate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Active site","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":241,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QJC19243.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":309,"modifier":"EXACT"},"end":{"value":309,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QJC19243.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003370"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022532"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003370"}],"id":"KW-0816","category":"Biological process","name":"Tricarboxylic acid cycle"}],"references":[{"referenceNumber":1,"citation":{"id":"31918751","citationType":"journal article","authors":["Zhao S.","Yang M.","Liu G.","Hornok S.","Zhao S.","Sang C.","Tan W.","Wang Y."],"citationCrossReferences":[{"database":"PubMed","id":"31918751"}],"title":"Rickettsiae in the common pipistrelle Pipistrellus pipistrellus (Chiroptera: Vespertilionidae) and the bat soft tick Argas vespertilionis (Ixodida: Argasidae).","publicationDate":"2020","journal":"Parasit. Vectors","firstPage":"0","lastPage":"10","volume":"13"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QJC19243.1"}],"value":"ZS17","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QJC19243.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"MN388799","properties":[{"key":"ProteinId","value":"QJC19243.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A6M3QJ09","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00223","properties":[{"key":"RectionId","value":"UER00717"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0036440","properties":[{"key":"GoTerm","value":"F:citrate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006099","properties":[{"key":"GoTerm","value":"P:tricarboxylic acid cycle"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"FunFam","id":"1.10.230.10:FF:000002","properties":[{"key":"EntryName","value":"Citrate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.580.10","properties":[{"key":"EntryName","value":"Citrate Synthase, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.230.10","properties":[{"key":"EntryName","value":"Cytochrome P450-Terp, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR016142","properties":[{"key":"EntryName","value":"Citrate_synth-like_lrg_a-sub"}]},{"database":"InterPro","id":"IPR016143","properties":[{"key":"EntryName","value":"Citrate_synth-like_sm_a-sub"}]},{"database":"InterPro","id":"IPR002020","properties":[{"key":"EntryName","value":"Citrate_synthase"}]},{"database":"InterPro","id":"IPR019810","properties":[{"key":"EntryName","value":"Citrate_synthase_AS"}]},{"database":"InterPro","id":"IPR036969","properties":[{"key":"EntryName","value":"Citrate_synthase_sf"}]},{"database":"InterPro","id":"IPR010953","properties":[{"key":"EntryName","value":"Citrate_synthase_typ-I"}]},{"database":"NCBIfam","id":"TIGR01798","properties":[{"key":"EntryName","value":"cit_synth_I"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF004126","properties":[{"key":"EntryName","value":"PRK05614.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42871","properties":[{"key":"EntryName","value":"CITRATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42871:SF1","properties":[{"key":"EntryName","value":"CITRATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00285","properties":[{"key":"EntryName","value":"Citrate_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00143","properties":[{"key":"EntryName","value":"CITRTSNTHASE"}]},{"database":"SUPFAM","id":"SSF48256","properties":[{"key":"EntryName","value":"Citrate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00480","properties":[{"key":"EntryName","value":"CITRATE_SYNTHASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"GILRHRGYDIKDLAEKSDFLEVAYLLIYGELPSGEQYNNFTKQVAHHSLVNERLHYLFQTFCSSSHPMAIMLAAVGSLAAFYPDLLNFKEADYELTAIRMIAKIPTIAAMSYKYSIGQPFIYPDNSLDFTENFLHMMFATPCTKYTVNPIIKNALNKIFILHADHEQNASTSTVRIAGSSGANPFACISTGIASLWGPAHGGANEAVINMLKEIGSSEYIPKYIAKAKDKNDPFRLMGFGHRVYKNYDPRAVVLKETCKEVLKELGQLDNNLLQIAIELEAIALKDEYFIERKLYPNVDFYSGIIYKAM","length":309,"molWeight":34777,"crc64":"BEF46DB96AE4F942","md5":"9249DDC7A457576096956D7E1EA8CB93"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":2,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI0014526039"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A7S9VL65","uniProtkbId":"A0A7S9VL65_RICRI","entryAudit":{"firstPublicDate":"2021-06-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2021-06-02","entryVersion":13,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Rickettsia rickettsii","taxonId":783,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QPI69480.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Alphaproteobacteria","Rickettsiales","Rickettsiaceae","Rickettsieae","Rickettsia","spotted fever group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}],"value":"Citrate (Si)-synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}],"value":"2.3.3.1"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QPI69480.1"}],"value":"gltA"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16845"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16452"},{"database":"ChEBI","id":"CHEBI:16947"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"}],"ecNumber":"2.3.3.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004751"}],"value":"Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010566"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003406"}],"value":"Belongs to the citrate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Active site","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10117"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QPI69480.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QPI69480.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003406"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"33208186","citationType":"journal article","authors":["Hoque M.M.","Barua S.","Kelly P.J.","Chenoweth K.","Kaltenboeck B.","Wang C."],"citationCrossReferences":[{"database":"PubMed","id":"33208186"}],"title":"Identification of Rickettsia felis DNA in the blood of domestic cats and dogs in the USA.","publicationDate":"2020","journal":"Parasit. Vectors","firstPage":"0","lastPage":"581","volume":"13"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QPI69480.1"}],"value":"Kebar-2","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QPI69480.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"MT633738","properties":[{"key":"ProteinId","value":"QPI69480.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A7S9VL65","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00223","properties":[{"key":"RectionId","value":"UER00717"}]},{"database":"GO","id":"GO:0036440","properties":[{"key":"GoTerm","value":"F:citrate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006099","properties":[{"key":"GoTerm","value":"P:tricarboxylic acid cycle"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"Gene3D","id":"1.10.230.10","properties":[{"key":"EntryName","value":"Cytochrome P450-Terp, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR016143","properties":[{"key":"EntryName","value":"Citrate_synth-like_sm_a-sub"}]},{"database":"InterPro","id":"IPR002020","properties":[{"key":"EntryName","value":"Citrate_synthase"}]},{"database":"InterPro","id":"IPR019810","properties":[{"key":"EntryName","value":"Citrate_synthase_AS"}]},{"database":"InterPro","id":"IPR036969","properties":[{"key":"EntryName","value":"Citrate_synthase_sf"}]},{"database":"PANTHER","id":"PTHR42871","properties":[{"key":"EntryName","value":"CITRATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42871:SF1","properties":[{"key":"EntryName","value":"CITRATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00285","properties":[{"key":"EntryName","value":"Citrate_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00143","properties":[{"key":"EntryName","value":"CITRTSNTHASE"}]},{"database":"SUPFAM","id":"SSF48256","properties":[{"key":"EntryName","value":"Citrate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00480","properties":[{"key":"EntryName","value":"CITRATE_SYNTHASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"VRIAGSSGANPFACISTGIASLWGPAHGGANEAVINMLKEIGSSEYIPKYIAKAKDKNDPFRLMGFGHRVYKNYDPRAVVLKETCKEVLKELGQLDNNPL","length":100,"molWeight":10863,"crc64":"81D64DA13762C625","md5":"B8C0E6C0CDC81D516EB83130D0F225A7"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI0019376B14"}}]}