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Not inhibited by the coenzyme M analog 2-bromoethanesulfonate (BES) (PubMed:20551308)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":1.8,"unit":"uM","substrate":"R-epoxypropane","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11939797"}]},{"constant":33.6,"unit":"uM","substrate":"coenzyme M","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11939797"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11939797"}],"value":"kcat is 6.5 sec(-1) with R-epoxypropane as substrate."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11939797"}],"value":"Optimum pH is 8.0-8.5."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10411892"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9405410"}],"value":"Alkene metabolism; propylene degradation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10411892"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9405410"}],"value":"Homohexamer (PubMed:9405410). Component I of the aliphatic epoxide carboxylation complex together with components II, III and IV (PubMed:10411892, PubMed:9405410)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the vitamin-B12 independent methionine synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":376,"modifier":"EXACT"}},"description":"2-hydroxypropyl-CoM lyase","featureId":"PRO_0000098705"},{"type":"Binding site","location":{"start":{"value":218,"modifier":"EXACT"},"end":{"value":218,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"11939797"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":341,"modifier":"EXACT"},"end":{"value":341,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Mutagenesis","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"Retains 0.06% of wild-type specific activity. 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III"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20302306"}],"value":"RHPCDH1"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20302306"}],"value":"xecD1"},"orderedLocusNames":[{"value":"Xaut_4868"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10411892"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11851420"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15157110"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20302306"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9405410"}],"value":"Involved in aliphatic epoxide carboxylation (PubMed:10411892, PubMed:11851420, PubMed:9405410). Catalyzes the reversible oxidation of (R)-2-hydroxypropyl-coenzyme M (R-HPC) to 2-oxopropyl-coenzyme M (2-KPC) (PubMed:10411892, PubMed:11851420, PubMed:15157110, PubMed:20302306). The enzyme is highly specific for the R enantiomers (PubMed:10411892, PubMed:15157110, PubMed:20302306). In vitro can also use achiral 2-propanol and short-chain (R)- and (S)-2-alkanols (PubMed:15157110)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(R)-2-hydroxypropyl-coenzyme M + NAD(+) = 2-oxopropyl-coenzyme M + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13249"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57552"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:58458"}],"ecNumber":"1.1.1.268","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10411892"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11851420"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15157110"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20302306"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:13250"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10411892"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11851420"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15157110"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20302306"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11851420"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20302306"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20551308"}],"value":"Inhibited by the arginine-specific modifiers 2,3-butanedione and phenylglyoxal (PubMed:11851420). 2-(2-methyl-2-hydroxypropylthio)ethanesulfonate (M-HPC), an achiral analog of both R-HPC and S-HPC, and (2S)-2-hydroxypropyl-coenzyme M (S-HPC) are competitive inhibitors (PubMed:11851420, PubMed:20302306). 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