{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q15KI8","uniProtkbId":"PMST1_PASMD","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2006-07-25","entryVersion":80,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Pasteurella multocida","taxonId":747,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Pasteurellales","Pasteurellaceae","Pasteurella"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Alpha-2,3-sialyltransferase/sialidase"},"shortNames":[{"value":"PmST1"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16351087"}],"value":"2.4.3.-"},{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16351087"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35872821"}],"value":"2.4.3.6"},{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16351087"}],"value":"3.2.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16351087"}],"value":"Pm0188 protein homolog"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16351087"}],"value":"Pm0188Ph"}]}],"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16351087"}],"value":"Pm0188"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16351087"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17487984"}],"value":"Multifunctional sialyltransferase/sialidase with flexible donnor and acceptor substrate specificity. In vitro at pH 7.5-9, catalyzes the transfer of sialic acid (N-acetyl-neuraminic acid; Neu5Ac) from the nucleotide sugar donor CMP-Neu5Ac onto galactosides through an alpha2,3 linkage. Efficiently sialylates N-acetyllactosamine, lactose, alpha- and beta-D-galactose substrates. Alternatively, can form alpha2,3 sialyl linkages using CMP-N-glycoloyl-beta-neuraminic acid (CMP-Neu5Gc) and CMP-beta-3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate (CMP-KDN) as donnor substrates. Changes regioselectivity at acidic pH forming alpha2,6-linked sialosides, albeit with much lower efficiency. Does not sialylate GalNAc-terminated glycoconjugates (PubMed:16351087, PubMed:17487984). Displays sialidase and trans-sialidase activities specifically cleaving alpha2,3 sialyl linkages while leaving alpha2,6 sialyl linkages intact. Catalyzes trans-sialidation transferring sialyl residues from an alpha2,3-linked sialylgalactoside to another galactoside (PubMed:16351087)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16351087"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35872821"}],"value":"In host-pathogen interactions, may produce molecular mimics of host sialo-glycans or modify them to evade the host immune system and enhance bacterial virulence"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + CMP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:52316"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57812"},{"database":"ChEBI","id":"CHEBI:60377"},{"database":"ChEBI","id":"CHEBI:133507"},{"database":"ChEBI","id":"CHEBI:136545"}],"ecNumber":"2.4.3.6","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16351087"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35872821"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + 2 CMP-N-acetyl-beta-neuraminate = an N(4)-{alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + 2 CMP + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:85215"},{"database":"Rhea","id":"RHEA-COMP:20501"},{"database":"Rhea","id":"RHEA-COMP:20524"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57812"},{"database":"ChEBI","id":"CHEBI:60377"},{"database":"ChEBI","id":"CHEBI:234185"},{"database":"ChEBI","id":"CHEBI:234247"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35872821"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + CMP-N-acetyl-beta-neuraminate = an N(4)-{alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + CMP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:85219"},{"database":"Rhea","id":"RHEA-COMP:20503"},{"database":"Rhea","id":"RHEA-COMP:20528"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57812"},{"database":"ChEBI","id":"CHEBI:60377"},{"database":"ChEBI","id":"CHEBI:234193"},{"database":"ChEBI","id":"CHEBI:234251"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35872821"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + CMP-N-acetyl-beta-neuraminate = an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + CMP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:85231"},{"database":"Rhea","id":"RHEA-COMP:20502"},{"database":"Rhea","id":"RHEA-COMP:20530"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57812"},{"database":"ChEBI","id":"CHEBI:60377"},{"database":"ChEBI","id":"CHEBI:234194"},{"database":"ChEBI","id":"CHEBI:234253"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35872821"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"an N(4)-{alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + CMP-N-acetyl-beta-neuraminate = an N(4)-{alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + CMP + 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N(4)-{alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + CMP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:85239"},{"database":"Rhea","id":"RHEA-COMP:20504"},{"database":"Rhea","id":"RHEA-COMP:20525"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57812"},{"database":"ChEBI","id":"CHEBI:60377"},{"database":"ChEBI","id":"CHEBI:234195"},{"database":"ChEBI","id":"CHEBI:234249"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35872821"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":0.44,"unit":"mM","substrate":"CMP-N-acetyl-beta-neuraminate (alpha2,3-sialyltransferase 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(PubMed:16351087). kcat is 47 sec(-1) for LacMU (alpha2,3-sialyltransferase activity) (PubMed:16351087). kcat is 227 sec(-1) for alpha-Neu5Ac-(2->3)-beta-D-galactosyl-(1-4)-beta-D-glucoside, Neu5Acalpha2,3-LacMU (alpha2,3-sialidase activity) (PubMed:16351087). kcat is 31.6 sec(-1) for CMP-N-acetyl-beta-neuraminate (alpha2,3-sialyltransferase activity) (PubMed:17487984)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16351087"}],"value":"Optimum pH is 7.5-9 for alpha2,3 sialylation, 4.5-7 for alpha2,6 sialylation and 5-6.5 for sialidase/trans-sialidase activity."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16351087"}],"value":"Glycan 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As part of a multi-enzymatic cascade, it is used for generation of a library of sialylated bisected N-glycans naturally involved in immune recognition and cell-cell communication"}],"commentType":"BIOTECHNOLOGY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase 80 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":412,"modifier":"EXACT"}},"description":"Alpha-2,3-sialyltransferase/sialidase","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000463692"},{"type":"Coiled coil","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Binding 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