{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A1W7HCY1","uniProtkbId":"IUTB_VIBVL","entryAudit":{"firstPublicDate":"2023-09-13","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2017-07-05","entryVersion":15,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAX35098.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28150143"}],"value":"Ferric aerobactin reductase IutB"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"1.16.1.10"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"}],"value":"Ferric chelate reductase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"}],"value":"FCR"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAX35098.1"}],"value":"Ferric reductase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28150143"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAX35098.1"}],"value":"iutB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28150143"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"},{"evidenceCode":"ECO:0000305"}],"value":"Ferric-siderophore reductase involved in iron removal from the siderophores after their transport into the cell (Probable). Acts as a major ferric-aerobactin reductase catalyzing the reduction of Fe(3+)-aerobactin, a citrate-hydroxamate siderophore produced by other bacteria. Catalyzes reduction of Fe(3+)-vulnibactin, a catecholate siderophore synthesized by V.vulnificus, in the absence of VuuB (PubMed:28150143, PubMed:32681432). Catalyzes reduction of ferrioxamine B and Fe(3+)-vibriobactin in vitro. No activity with Fe(3+)-enterobactin. Catalyzes reduction of ferric chelating compound Fe(3+)-nitrilotriacetic acid (NTA) in the presence of NADH, NADPH or reduced glutathione (GSH) as its electron donor in vitro. Also catalyzes reduction of ferric chelating compounds Fe(3+)-citrate and Fe(3+)-EDTA as well as non-complexed FeCl3 in the presence of GSH as its electron donor in vitro. Highest activity with Fe(3+)-NTA as electron acceptor and GSH as donor (PubMed:32681432)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 a Fe(II)-siderophore + NAD(+) + H(+) = 2 a Fe(III)-siderophore + NADH","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15061"},{"database":"Rhea","id":"RHEA-COMP:11342"},{"database":"Rhea","id":"RHEA-COMP:11344"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29033"},{"database":"ChEBI","id":"CHEBI:29034"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"1.16.1.10","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:15063"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:28795"},{"database":"Rhea","id":"RHEA-COMP:11342"},{"database":"Rhea","id":"RHEA-COMP:11344"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29033"},{"database":"ChEBI","id":"CHEBI:29034"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"ecNumber":"1.16.1.10","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:28797"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"[2Fe-2S] cluster","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P39405"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:190135"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P39405"}],"value":"Binds 1 [2Fe-2S] cluster."}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":6.83,"unit":"nmol/min/mg","enzyme":"enzyme with Fe(3+)-aerobactin as substrate and glutathione (GSH) as electron donor (at pH 8.0 and 30 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},{"velocity":2.32,"unit":"nmol/min/mg","enzyme":"enzyme with Fe(3+)-vulnibactin as substrate and glutathione (GSH) as electron donor (at pH 8.0 and 30 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},{"velocity":1.44,"unit":"nmol/min/mg","enzyme":"enzyme with ferrioxamine B as substrate and glutathione (GSH) as electron donor (at pH 8.0 and 30 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},{"velocity":1.1,"unit":"nmol/min/mg","enzyme":"enzyme with Fe(3+)-vibriobactin as substrate and glutathione (GSH) as electron donor (at pH 8.0 and 30 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},{"velocity":10.98,"unit":"nmol/min/mg","enzyme":"enzyme with Fe(3+)-nitrilotriacetic acid (NTA) as electron acceptor and glutathione (GSH) as electron donor (at pH 8.0 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},{"velocity":14.24,"unit":"nmol/min/mg","enzyme":"enzyme with Fe(3+)-nitrilotriacetic acid (NTA) as electron acceptor and simultaneous presence of glutathione (GSH) and NADPH as electron donors (at pH 8.0 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]}]},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"Optimum pH is 8.5 with Fe(3+)-nitrilotriacetic acid (NTA) as electron acceptor."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"Optimum temperature is 45 degrees Celsius with Fe(3+)-nitrilotriacetic acid (NTA) as electron acceptor."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28150143"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28150143"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"Expression is up-regulated by low iron concentration condition. Expression is repressed by Fur under iron-repleted condition"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28150143"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"No effect on growth under low iron condition. No effect on vulnibactin synthesis or secretion. Double deletion iutB/vuuB mutant secretes vulnibactin as wild-type, but has a significant growth impairment under low iron condition. Triple deletion iutB/vuuB/ics mutant supplemented with aerobactin has a significant growth impairment under low iron condition (PubMed:28150143). Double deletion iutB/ics mutant does not grow under low iron condition, but addition of vulnibactin restores the growth (PubMed:32681432)"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":244,"modifier":"EXACT"}},"description":"Ferric aerobactin reductase IutB","featureId":"PRO_0000458466"},{"type":"Binding site","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P39405"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}},{"type":"Binding site","location":{"start":{"value":221,"modifier":"EXACT"},"end":{"value":221,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P39405"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}},{"type":"Binding site","location":{"start":{"value":232,"modifier":"EXACT"},"end":{"value":232,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P39405"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}},{"type":"Binding site","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:190135"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P39405"}],"ligand":{"name":"[2Fe-2S] cluster","id":"ChEBI:CHEBI:190135"}}],"keywords":[{"id":"KW-0001","category":"Ligand","name":"2Fe-2S"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0520","category":"Ligand","name":"NAD"},{"id":"KW-0521","category":"Ligand","name":"NADP"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"28150143","citationType":"journal article","authors":["Kawano H.","Miyamoto K.","Negoro M.","Zushi E.","Tsuchiya T.","Tanabe T.","Funahashi T.","Tsujibo H."],"citationCrossReferences":[{"database":"PubMed","id":"28150143"},{"database":"DOI","id":"10.1007/s10534-017-9994-0"}],"title":"IutB participates in the ferric-vulnibactin utilization system in Vibrio vulnificus M2799.","publicationDate":"2017","journal":"BioMetals","firstPage":"203","lastPage":"216","volume":"30"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","SUBCELLULAR LOCATION","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28150143"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAX35098.1"}],"value":"M2799","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAX35098.1"}]},{"referenceNumber":2,"citation":{"id":"32681432","citationType":"journal article","authors":["Okai N.","Miyamoto K.","Tomoo K.","Tsuchiya T.","Komano J.","Tanabe T.","Funahashi T.","Tsujibo H."],"citationCrossReferences":[{"database":"PubMed","id":"32681432"},{"database":"DOI","id":"10.1007/s10534-020-00241-5"}],"title":"VuuB and IutB reduce ferric-vulnibactin in Vibrio vulnificus M2799.","publicationDate":"2020","journal":"BioMetals","firstPage":"187","lastPage":"200","volume":"33"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","SUBSTRATE SPECIFICITY","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"}],"value":"M2799","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LC194887","properties":[{"key":"ProteinId","value":"BAX35098.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1W7HCY1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AALHEYW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"28150143"}]},{"database":"GO","id":"GO:0051537","properties":[{"key":"GoTerm","value":"F:2 iron, 2 sulfur cluster binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0140618","properties":[{"key":"GoTerm","value":"F:ferric-chelate reductase (NADH) activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32681432"}]},{"database":"GO","id":"GO:0052851","properties":[{"key":"GoTerm","value":"F:ferric-chelate reductase (NADPH) activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32681432"}]},{"database":"GO","id":"GO:0010106","properties":[{"key":"GoTerm","value":"P:cellular response to iron ion starvation"},{"key":"GoEvidenceType","value":"IEP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000270","source":"PubMed","id":"28150143"},{"evidenceCode":"ECO:0000270","source":"PubMed","id":"32681432"}]},{"database":"InterPro","id":"IPR023998","properties":[{"key":"EntryName","value":"FCR-like"}]},{"database":"InterPro","id":"IPR024726","properties":[{"key":"EntryName","value":"FhuF_C"}]},{"database":"NCBIfam","id":"TIGR03950","properties":[{"key":"EntryName","value":"sidero_Fe_reduc"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11575","properties":[{"key":"EntryName","value":"FhuF_C"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSGHSFFEHLFEHSQHVTPYLHGAIKPRPERCAEHGFIHIEHASSDHIRALYESLKLAHPEAGAAYWLTRTWTLLCWQPLYVAFIAIYSCQGLPKLSSMGQHVQPRFVSGYQFDDDEYRQGSEQELIAHAGKELCALFDYFRQEMSLWTRIRPGFTQHLFADGVFGCLVKLSQFYPTLSGDYFLEQARLWLAACQLPEKLIQSLRYDETSRQLSLVRTSCCLVYKCQGRELCRDCPRHPDNKRE","length":244,"molWeight":28349,"crc64":"C907FEBC57460B02","md5":"2B54E2A9550189401FA7EC6559D62437"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1,"Binding site":4},"uniParcId":"UPI000A1C7733"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9L9Z9","uniProtkbId":"VUUA_VIBVL","entryAudit":{"firstPublicDate":"2023-09-13","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2000-10-01","entryVersion":110,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAF28471.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10639413"}],"value":"Ferric vulnibactin receptor VuuA"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAF28471.1"}],"value":"Vulnibactin outer membrane receptor"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10639413"}],"value":"Vulnibactin uptake protein A"}}],"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10639413"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAF28471.1"}],"value":"vuuA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10639413"},{"evidenceCode":"ECO:0000305"}],"value":"Involved in the uptake of iron in complex with vulnibactin, a catecholate siderophore synthesized by V.vulnificus. Binds and transports ferric vulnibactin across the outer membrane (PubMed:10639413). The energy source is provided by the inner membrane TonB system (Probable)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01360"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10639413"}],"value":"Cell outer membrane","id":"SL-0040"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01360"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10639413"}],"value":"Expression is up-regulated by low iron concentration condition. Iron-regulated expression is repressed by Fur"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10639413"}],"value":"Unable to use transferrin or ferric vulnibactin as iron source, but able to use heme, hemoglobin and FeSO4 as iron source. Reduced virulence (increased LD(50)) in infant mouse model"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01360"}],"value":"Belongs to the TonB-dependent receptor family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10639413"}]},{"type":"Chain","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":687,"modifier":"EXACT"}},"description":"Ferric vulnibactin receptor VuuA","evidences":[{"evidenceCode":"ECO:0000255"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"10639413"}],"featureId":"PRO_5009973925"},{"type":"Domain","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"TBDR plug","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01360"}]},{"type":"Domain","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":687,"modifier":"EXACT"}},"description":"TBDR beta-barrel","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01360"}]},{"type":"Motif","location":{"start":{"value":670,"modifier":"EXACT"},"end":{"value":687,"modifier":"EXACT"}},"description":"TonB C-terminal box","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01360"},{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10144"}]}],"keywords":[{"id":"KW-0998","category":"Cellular component","name":"Cell outer membrane"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0410","category":"Biological process","name":"Iron transport"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0675","category":"Molecular function","name":"Receptor"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0798","category":"Domain","name":"TonB box"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1134","category":"Domain","name":"Transmembrane beta strand"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"10639413","citationType":"journal article","authors":["Webster A.C.","Litwin C.M."],"citationCrossReferences":[{"database":"PubMed","id":"10639413"},{"database":"DOI","id":"10.1128/iai.68.2.526-534.2000"}],"title":"Cloning and characterization of vuuA, a gene encoding the Vibrio vulnificus ferric vulnibactin receptor.","publicationDate":"2000","journal":"Infect. Immun.","firstPage":"526","lastPage":"534","volume":"68"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","PROTEIN SEQUENCE OF 38-53","FUNCTION","SUBCELLULAR LOCATION","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10639413"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAF28471.1"}],"value":"MO6-24","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAF28471.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF156494","properties":[{"key":"ProteinId","value":"AAF28471.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_015728225.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP014048.2"}]},{"database":"AlphaFoldDB","id":"Q9L9Z9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9L9Z9","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ADWTHYS","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PHI-base","id":"PHI:3431","properties":[{"key":"Description","value":"-"}]},{"database":"PHI-base","id":"PHI:3649","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009279","properties":[{"key":"GoTerm","value":"C:cell outer membrane"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"10639413"}]},{"database":"GO","id":"GO:0015092","properties":[{"key":"GoTerm","value":"F:high-affinity ferric iron transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"10639413"}]},{"database":"GO","id":"GO:0015344","properties":[{"key":"GoTerm","value":"F:siderophore uptake transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"10639413"}]},{"database":"GO","id":"GO:0015343","properties":[{"key":"GoTerm","value":"F:siderophore-iron transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"10639413"}]},{"database":"GO","id":"GO:0038023","properties":[{"key":"GoTerm","value":"F:signaling receptor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0015891","properties":[{"key":"GoTerm","value":"P:siderophore transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0033214","properties":[{"key":"GoTerm","value":"P:siderophore-iron import into cell"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"10639413"}]},{"database":"Gene3D","id":"2.40.170.20","properties":[{"key":"EntryName","value":"TonB-dependent receptor, beta-barrel domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.170.130.10","properties":[{"key":"EntryName","value":"TonB-dependent receptor, plug domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036942","properties":[{"key":"EntryName","value":"Beta-barrel_TonB_sf"}]},{"database":"InterPro","id":"IPR012910","properties":[{"key":"EntryName","value":"Plug_dom"}]},{"database":"InterPro","id":"IPR037066","properties":[{"key":"EntryName","value":"Plug_dom_sf"}]},{"database":"InterPro","id":"IPR039426","properties":[{"key":"EntryName","value":"TonB-dep_rcpt-like"}]},{"database":"InterPro","id":"IPR010917","properties":[{"key":"EntryName","value":"TonB_rcpt_CS"}]},{"database":"InterPro","id":"IPR010105","properties":[{"key":"EntryName","value":"TonB_sidphr_rcpt"}]},{"database":"NCBIfam","id":"TIGR01783","properties":[{"key":"EntryName","value":"TonB-siderophor"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32552","properties":[{"key":"EntryName","value":"FERRICHROME IRON RECEPTOR-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32552:SF81","properties":[{"key":"EntryName","value":"TONB-DEPENDENT OUTER MEMBRANE RECEPTOR"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07715","properties":[{"key":"EntryName","value":"Plug"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56935","properties":[{"key":"EntryName","value":"Porins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01156","properties":[{"key":"EntryName","value":"TONB_DEPENDENT_REC_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52016","properties":[{"key":"EntryName","value":"TONB_DEPENDENT_REC_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAALRPARTSVAEKKTFKLHALSAVVMGLCASGQAYAQTESTNSNKKEEMPVVVVIGEKTERTIYDTSSSVQVFDQETIDNTPGATEIDDLLQLIPNMVDSGQGNSMPTVRGIDGSGPSIGGLASFAGTSPRLNMSIDGRSLTYSEIAFGPRSLWDMQQVEVYLGPQSYIQGRNASAGAIVMKTNDPTHHFESAVKAGVGERNYSQTAAMISAPIIQDELAFRLSFDQQKRDSFVDLASYEPAGDAKKIEMNSVRGKLLYEPSALAGFKTTLGVSHMDSRGPQSESTNVVGNEAFRPVYETKSLSTAWDISWQLNEVLTFENNLVYSKFAFDRYTNPLQKGDYTAEGKEFHVEPLLRYLSLGGRVNALVGARYYKSSQDDEYVDATSANPMSGSTKTQSAFAELTYALTQSIDVTVAGRYEKERVKRKVSDPRFKLDHDDTLSVFLPKFDIAFKPDMAQTFGFKVAKGYNSGGAGLAFNPILGGGFSPYQFEEEYIWNYEFYTRHRLGNSVELMTNTFYNDFDSMQMTQTLSNGDVLIANLDNAKTYGAEIGTRWYATDSLELFANLGLLKTEYKEVNGTSKELERAPNMTGNLGGQYSFFDGFELSANAAYTGDYFSDRSNTEIVKIDAYWVANAQLAYVFENGRAALFATNLFDSDKTTLYARGSLNEPLKQQPRMIGASLQLNF","length":687,"molWeight":75829,"crc64":"E12F8423DDC8CABC","md5":"A5F7974106BCB83B566EDB37D6130014"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":2,"Motif":1},"uniParcId":"UPI00000B2571"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"V5XKC3","uniProtkbId":"VUUB_VIBVL","entryAudit":{"firstPublicDate":"2023-09-13","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2014-02-19","entryVersion":26,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAO05510.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28150143"}],"value":"Ferric vulnibactin reductase VuuB"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"1.16.1.7"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"}],"value":"Ferric chelate reductase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"}],"value":"FCR"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24135791"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"}],"value":"Ferric reductase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24135791"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAO05510.1"}],"value":"Ferric-vulnibactin utilization protein"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"}],"value":"Vulnibactin utilization protein B"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24135791"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28150143"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAO05510.1"}],"value":"vuuB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28150143"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"},{"evidenceCode":"ECO:0000305"}],"value":"Ferric-siderophore reductase involved in iron removal from the siderophores after their transport into the cell (Probable). Acts as a major ferric-vulnibactin reductase catalyzing the reduction of Fe(3+)-vulnibactin, a catecholate siderophore synthesized by V.vulnificus. Catalyzes reduction of Fe(3+)-aerobactin, a citrate-hydroxamate siderophore produced by other bacteria, in the absence of IutB (PubMed:28150143, PubMed:32681432). Catalyzes reduction of Fe(3+)-vibriobactin in vitro. No activity with ferrioxamine B or Fe(3+)-enterobactin. Catalyzes reduction of ferric chelating compounds Fe(3+)-nitrilotriacetic acid (NTA), Fe(3+)-citrate and Fe(3+)-EDTA as well as non-complexed FeCl3 in the presence of NADH as its electron donor and FAD as its cofactor in vitro. Highest activity with Fe(3+)-NTA as electron acceptor (PubMed:32681432)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 a Fe(II)-siderophore + NAD(+) + H(+) = 2 a Fe(III)-siderophore + NADH","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15061"},{"database":"Rhea","id":"RHEA-COMP:11342"},{"database":"Rhea","id":"RHEA-COMP:11344"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29033"},{"database":"ChEBI","id":"CHEBI:29034"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"1.16.1.7","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:15063"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"FAD","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:57692"}}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":1.72,"unit":"nmol/min/mg","enzyme":"enzyme with Fe(3+)-vulnibactin as substrate (at pH 7.0 and 30 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},{"velocity":1.73,"unit":"nmol/min/mg","enzyme":"enzyme with Fe(3+)-aerobactin as substrate (at pH 7.0 and 30 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},{"velocity":0.97,"unit":"nmol/min/mg","enzyme":"enzyme with Fe(3+)-vibriobactin as substrate (at pH 7.0 and 30 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]},{"velocity":25.89,"unit":"nmol/min/mg","enzyme":"enzyme with Fe(3+)-nitrilotriacetic acid (NTA) as electron acceptor (at pH 7.0 and 30 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}]}]},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"Optimum pH is 7.0 with Fe(3+)-nitrilotriacetic acid (NTA) as electron acceptor."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"Optimum temperature is 37 degrees Celsius with Fe(3+)-nitrilotriacetic acid (NTA) as electron acceptor."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28150143"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24135791"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"Expression is up-regulated by low iron concentration condition. Expression is repressed by Fur under iron-repleted condition"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24135791"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28150143"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32681432"}],"value":"Slightly impaired growth under low iron condition (PubMed:24135791, PubMed:28150143). Double deletion vuuB/iutB mutant secretes vulnibactin as wild-type, but has a significant growth impairment under low iron condition. Triple deletion vuuB/iutB/ics mutant supplemented with aerobactin has a significant growth impairment under low iron condition (PubMed:28150143). Double deletion vuub/ics mutant does not grow under low iron condition, but addition of vulnibactin restores the growth (PubMed:32681432)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the SIP oxidoreductase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"Ferric vulnibactin reductase VuuB","featureId":"PRO_0000458467"},{"type":"Domain","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"FAD-binding FR-type","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00716"}]}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0274","category":"Ligand","name":"FAD"},{"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"id":"KW-0520","category":"Ligand","name":"NAD"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"24135791","citationType":"journal article","authors":["Kawano H.","Miyamoto K.","Sakaguchi I.","Myojin T.","Moriwaki M.","Tsuchiya T.","Tanabe T.","Yamamoto S.","Tsujibo H."],"citationCrossReferences":[{"database":"PubMed","id":"24135791"},{"database":"DOI","id":"10.1016/j.micpath.2013.10.002"}],"title":"Role of periplasmic binding proteins, FatB and VatD, in the vulnibactin utilization system of Vibrio vulnificus M2799.","publicationDate":"2013","journal":"Microb. Pathog.","firstPage":"73","lastPage":"81","volume":"65"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24135791"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAO05510.1"}],"value":"M2799","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAO05510.1"}]},{"referenceNumber":2,"citation":{"id":"28150143","citationType":"journal article","authors":["Kawano H.","Miyamoto K.","Negoro M.","Zushi E.","Tsuchiya T.","Tanabe T.","Funahashi T.","Tsujibo H."],"citationCrossReferences":[{"database":"PubMed","id":"28150143"},{"database":"DOI","id":"10.1007/s10534-017-9994-0"}],"title":"IutB participates in the ferric-vulnibactin utilization system in Vibrio vulnificus M2799.","publicationDate":"2017","journal":"BioMetals","firstPage":"203","lastPage":"216","volume":"30"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28150143"}],"value":"M2799","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"32681432","citationType":"journal article","authors":["Okai N.","Miyamoto K.","Tomoo K.","Tsuchiya T.","Komano J.","Tanabe T.","Funahashi T.","Tsujibo H."],"citationCrossReferences":[{"database":"PubMed","id":"32681432"},{"database":"DOI","id":"10.1007/s10534-020-00241-5"}],"title":"VuuB and IutB reduce ferric-vulnibactin in Vibrio vulnificus M2799.","publicationDate":"2020","journal":"BioMetals","firstPage":"187","lastPage":"200","volume":"33"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","SUBSTRATE SPECIFICITY","COFACTOR","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32681432"}],"value":"M2799","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB822515","properties":[{"key":"ProteinId","value":"BAO05510.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"V5XKC3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"V5XKC3","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"28150143"}]},{"database":"GO","id":"GO:0140618","properties":[{"key":"GoTerm","value":"F:ferric-chelate reductase (NADH) activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32681432"}]},{"database":"GO","id":"GO:0010106","properties":[{"key":"GoTerm","value":"P:cellular response to iron ion starvation"},{"key":"GoEvidenceType","value":"IEP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000270","source":"PubMed","id":"24135791"},{"evidenceCode":"ECO:0000270","source":"PubMed","id":"32681432"}]},{"database":"CDD","id":"cd06193","properties":[{"key":"EntryName","value":"siderophore_interacting"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.80:FF:000038","properties":[{"key":"EntryName","value":"Vibriobactin utilization protein ViuB"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.80","properties":[{"key":"EntryName","value":"Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR017927","properties":[{"key":"EntryName","value":"FAD-bd_FR_type"}]},{"database":"InterPro","id":"IPR039261","properties":[{"key":"EntryName","value":"FNR_nucleotide-bd"}]},{"database":"InterPro","id":"IPR017938","properties":[{"key":"EntryName","value":"Riboflavin_synthase-like_b-brl"}]},{"database":"InterPro","id":"IPR013113","properties":[{"key":"EntryName","value":"SIP_FAD-bd"}]},{"database":"InterPro","id":"IPR039374","properties":[{"key":"EntryName","value":"SIP_fam"}]},{"database":"InterPro","id":"IPR007037","properties":[{"key":"EntryName","value":"SIP_rossman_dom"}]},{"database":"PANTHER","id":"PTHR30157","properties":[{"key":"EntryName","value":"FERRIC REDUCTASE, NADPH-DEPENDENT"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30157:SF0","properties":[{"key":"EntryName","value":"NADPH-DEPENDENT FERRIC-CHELATE REDUCTASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08021","properties":[{"key":"EntryName","value":"FAD_binding_9"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04954","properties":[{"key":"EntryName","value":"SIP"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF63380","properties":[{"key":"EntryName","value":"Riboflavin synthase domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51384","properties":[{"key":"EntryName","value":"FAD_FR"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSDSPERVYPMLLDFVRKETISKNLLRVTLTGEDLIGFPEDQNGSHIKVFFPNQASGILQLPVREGDNVIWPEHKPVPRAYSVRQYRAAVNELDIDFVTHGEETPGGGWALKADIGSQIGLIGPAGPDPLIEPADWHIIAGDLSAVPAISAILEKLPSDAKGYVFIEVDEIEDIHDLVHPEEMAINWLMRNPHDTEPALAKAIKQLPSPEKATSLSAFIAGENQSVINCRKILRNDYQIARDKLYAIPYWKRGKTEEAYHDERHDVMDAVY","length":271,"molWeight":30323,"crc64":"E1951C4415E639E2","md5":"49ADE92645AE199142914D519807C3A3"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1},"uniParcId":"UPI0003C9A35E"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q845T3","uniProtkbId":"VATD_VIBVL","entryAudit":{"firstPublicDate":"2023-09-13","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2004-07-05","entryVersion":52,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAC65321.2"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24135791"}],"value":"Ferric aerobactin-binding protein VatD"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Iron(3+)-hydroxamate-binding protein VatD"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16172537"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24135791"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26249703"}],"value":"Periplasmic-binding protein VatD"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24135791"}],"value":"PBP VatD"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16172537"}],"value":"Vulnificus aerobactin transport protein D"}}],"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16172537"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"19185608"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24135791"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26249703"}],"value":"vatD"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAC65321.2"}],"value":"fhuD"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16172537"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24135791"}],"value":"Part of the ABC transporter complex VatCDB involved in the import of iron(3+)-complexed aerobactin, a citrate-hydroxamate siderophore produced by other bacteria. Binds the iron(3+)-aerobactin complex and transfers it to the membrane-bound permease (PubMed:16172537). Functions in the import of iron(3+)-complexed vulnibactin, a catecholate siderophore synthesized by V.vulnificus, in the absence of FatB (PubMed:24135791)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"The complex is composed of two ATP-binding proteins (VatC), two transmembrane proteins (VatB) and a solute-binding protein (VatD) (Probable)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Periplasm","id":"SL-0200"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19185608"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24135791"}],"value":"Expression is up-regulated by low iron concentration condition from early to mid logarithmic growth phase. Expression is repressed by Fur under iron-repleted condition"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16172537"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24135791"}],"value":"Impaired ability to utilize aerobactin as a heterologous siderophore (PubMed:16172537). No effect on growth. VatD/fatB double deletion mutant shows significantly greater growth impairment than single fatB mutant under low-iron concentration condition (PubMed:24135791)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the bacterial solute-binding protein 8 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":12,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"Ferric aerobactin-binding protein VatD","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000458490"},{"type":"Domain","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"Fe/B12 periplasmic-binding","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00344"}]},{"type":"Binding site","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:195191"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7W8F"}],"ligand":{"name":"desferrioxamine B","id":"ChEBI:CHEBI:195191"}},{"type":"Binding site","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:195191"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7W8F"}],"ligand":{"name":"desferrioxamine B","id":"ChEBI:CHEBI:195191"}},{"type":"Binding site","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:195191"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7W8F"}],"ligand":{"name":"desferrioxamine B","id":"ChEBI:CHEBI:195191"}},{"type":"Binding site","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:195191"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7W8F"}],"ligand":{"name":"desferrioxamine B","id":"ChEBI:CHEBI:195191"}},{"type":"Binding site","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":213,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:195191"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7W8F"}],"ligand":{"name":"desferrioxamine B","id":"ChEBI:CHEBI:195191"}},{"type":"Binding site","location":{"start":{"value":215,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:195191"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7W8F"}],"ligand":{"name":"desferrioxamine B","id":"ChEBI:CHEBI:195191"}},{"type":"Binding site","location":{"start":{"value":269,"modifier":"EXACT"},"end":{"value":269,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:195191"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7W8F"}],"ligand":{"name":"desferrioxamine B","id":"ChEBI:CHEBI:195191"}},{"type":"Binding site","location":{"start":{"value":271,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:195191"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.5"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7W8F"}],"ligand":{"name":"desferrioxamine B","id":"ChEBI:CHEBI:195191"}},{"type":"Beta strand","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":97,"modifier":"EXACT"},"end":{"value":102,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":103,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":169,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":180,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":183,"modifier":"EXACT"},"end":{"value":187,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":199,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":214,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":226,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":255,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Beta strand","location":{"start":{"value":262,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]},{"type":"Helix","location":{"start":{"value":274,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7W8F"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0410","category":"Biological process","name":"Iron transport"},{"id":"KW-0574","category":"Cellular component","name":"Periplasm"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"16172537","citationType":"journal article","authors":["Tanabe T.","Naka A.","Aso H.","Nakao H.","Narimatsu S.","Inoue Y.","Ono T.","Yamamoto S."],"citationCrossReferences":[{"database":"PubMed","id":"16172537"},{"database":"DOI","id":"10.1111/j.1348-0421.2005.tb03671.x"}],"title":"A novel aerobactin utilization cluster in Vibrio vulnificus with a gene involved in the transcription regulation of the iutA homologue.","publicationDate":"2005","journal":"Microbiol. Immunol.","firstPage":"823","lastPage":"834","volume":"49"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16172537"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAC65321.2"}],"value":"M2799","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAC65321.2"}]},{"referenceNumber":2,"citation":{"id":"19185608","citationType":"journal article","authors":["Miyamoto K.","Kosakai K.","Ikebayashi S.","Tsuchiya T.","Yamamoto S.","Tsujibo H."],"citationCrossReferences":[{"database":"PubMed","id":"19185608"},{"database":"DOI","id":"10.1016/j.micpath.2008.12.004"}],"title":"Proteomic analysis of Vibrio vulnificus M2799 grown under iron-repleted and iron-depleted conditions.","publicationDate":"2009","journal":"Microb. Pathog.","firstPage":"171","lastPage":"177","volume":"46"},"referencePositions":["INDUCTION","IDENTIFICATION BY MASS SPECTROMETRY"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"19185608"}],"value":"M2799","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24135791","citationType":"journal article","authors":["Kawano H.","Miyamoto K.","Sakaguchi I.","Myojin T.","Moriwaki M.","Tsuchiya T.","Tanabe T.","Yamamoto S.","Tsujibo H."],"citationCrossReferences":[{"database":"PubMed","id":"24135791"},{"database":"DOI","id":"10.1016/j.micpath.2013.10.002"}],"title":"Role of periplasmic binding proteins, FatB and VatD, in the vulnibactin utilization system of Vibrio vulnificus M2799.","publicationDate":"2013","journal":"Microb. Pathog.","firstPage":"73","lastPage":"81","volume":"65"},"referencePositions":["FUNCTION","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24135791"}],"value":"M2799","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"26249703","citationType":"journal article","authors":["Miyano N.","Igarashi T.","Kawano H.","Miyamoto K.","Tsuchiya T.","Tomoo K.","Tsujibo H."],"citationCrossReferences":[{"database":"PubMed","id":"26249703"},{"database":"DOI","id":"10.1107/s2053230x15011759"}],"title":"Expression, purification, crystallization and X-ray crystallographic analysis of the periplasmic binding protein VatD from Vibrio vulnificus M2799.","publicationDate":"2015","journal":"Acta Crystallogr. F Struct. Biol. Commun.","firstPage":"1078","lastPage":"1082","volume":"71"},"referencePositions":["CRYSTALLIZATION OF 14-292 UNCOMPLEXED AND IN COMPLEX WITH IRON(3+)-DESFERRIOXAMINE B"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26249703"}],"value":"M2799","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-6THM9BK4VB29A","citationType":"submission","authors":["Tomoo K.","Miyamoto K."],"title":"Crystal structure of siderophore binding protein VatD from Vibrio vulnificus M2799 complexed with Desferal.","publicationDate":"DEC-2021","submissionDatabase":"PDB data bank"},"referencePositions":["X-RAY CRYSTALLOGRAPHY IN COMPLEX WITH DESFERRIOXAMINE B; IRON AND MAGNESIUM"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.5"}],"value":"M2799","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"7W8F"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB074151","properties":[{"key":"ProteinId","value":"BAC65321.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PDB","id":"7W8F","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.90 A"},{"key":"Chains","value":"A=14-292"}]},{"database":"AlphaFoldDB","id":"Q845T3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q845T3","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ADFHHDP","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0043190","properties":[{"key":"GoTerm","value":"C:ATP-binding cassette (ABC) transporter complex"},{"key":"GoEvidenceType","value":"IC:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"16172537"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24135791"}]},{"database":"GO","id":"GO:0030288","properties":[{"key":"GoTerm","value":"C:outer membrane-bounded periplasmic space"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0042597","properties":[{"key":"GoTerm","value":"C:periplasmic space"},{"key":"GoEvidenceType","value":"IC:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"16172537"}]},{"database":"GO","id":"GO:0019271","properties":[{"key":"GoTerm","value":"P:aerobactin transport"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16172537"}]},{"database":"GO","id":"GO:0015687","properties":[{"key":"GoTerm","value":"P:ferric-hydroxamate import into cell"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16172537"}]},{"database":"GO","id":"GO:0006879","properties":[{"key":"GoTerm","value":"P:intracellular iron ion homeostasis"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16172537"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"24135791"}]},{"database":"GO","id":"GO:0006826","properties":[{"key":"GoTerm","value":"P:iron ion transport"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16172537"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"24135791"}]},{"database":"GO","id":"GO:0044718","properties":[{"key":"GoTerm","value":"P:siderophore transmembrane transport"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16172537"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"24135791"}]},{"database":"CDD","id":"cd01146","properties":[{"key":"EntryName","value":"FhuD"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1980","properties":[{"key":"EntryName","value":"Nitrogenase molybdenum iron protein domain"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR002491","properties":[{"key":"EntryName","value":"ABC_transptr_periplasmic_BD"}]},{"database":"InterPro","id":"IPR051313","properties":[{"key":"EntryName","value":"Bact_iron-sidero_bind"}]},{"database":"PANTHER","id":"PTHR30532","properties":[{"key":"EntryName","value":"IRON III DICITRATE-BINDING PERIPLASMIC PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30532:SF1","properties":[{"key":"EntryName","value":"IRON(3+)-HYDROXAMATE-BINDING PROTEIN FHUD"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01497","properties":[{"key":"EntryName","value":"Peripla_BP_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01715","properties":[{"key":"EntryName","value":"FERRIBNDNGPP"}]},{"database":"SUPFAM","id":"SSF53807","properties":[{"key":"EntryName","value":"Helical backbone' metal receptor"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50983","properties":[{"key":"EntryName","value":"FE_B12_PBP"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLSAALAFNSYALDITHEMGTTSFETTPKKVVALDWVLTETVLSLGIELEGAANISGYQQWVAEPHLNADAIDVGSRREPNLELLSNIKPDVILISKHLAAAYEPLSKIAPVLVYSVYSEDKQPLESAKRITRSLGKLFDKEQQAEQVIAQTDQRLAANGAKITSAGKAEKPLLFARFINDKTLRIHSEGSLAQDTINAMGLKNDWQEPTNLWGFTTTGTEKLAEHQKANVMIFGPLSQEERQQLTQSPLWQAMEFSRTDSVYELPAIWTFGGLLAAQRLSDHITGRLTQPQ","length":292,"molWeight":32318,"crc64":"0DD26AF1E9ADE6DE","md5":"EE0DF050E97597C16299DEA4C05670DD"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Binding site":8,"Beta strand":14,"Helix":13},"uniParcId":"UPI000035912F"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A2S3R7M0","uniProtkbId":"MARTX_VIBVL","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2018-07-18","entryVersion":29,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Multifunctional-autoprocessing repeats-in-toxin"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"MARTX"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"3.4.22.-"}]},"contains":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Actin cross-linking toxin F1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"6.3.2.-"}]}},{"recommendedName":{"fullName":{"value":"N-epsilon-fatty acyltransferase F2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29074776"}],"value":"2.3.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Rho inactivation domain-containing toxin F2"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29074776"}],"value":"RIDvc"}]}]},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"ABH effector region toxin F5"}}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Cysteine protease domain-containing toxin F3"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"3.4.22.-"}]}}],"flag":"Precursor"},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"POB49698.1"}],"value":"CRN52_02910"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Precursor of a multifunctional toxin that causes destruction of the actin cytoskeleton by covalent cross-linking of actin and inactivation of Rho GTPases when translocated into the host cytoplasm. Upon translocation into the host cell, undergoes autoprocessing in cis mediated by the peptidase C80 domain (also named CPD domain): the protease activity is activated upon binding inositol hexakisphosphate (InsP6) present at the host cell membrane and delivers the Cysteine protease domain-containing toxin F3 chain to the host cytosol. The Cysteine protease domain-containing toxin F3 chain will then further cleave and release effector toxin chains that cause disassembly of the actin cytoskeleton and enhance V.vulnificus colonization of the small intestine, possibly by facilitating evasion of phagocytic cells"}],"commentType":"FUNCTION","molecule":"Multifunctional-autoprocessing repeats-in-toxin"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Following autocatalytic cleavage in cis, this chain mediates processing in trans to release other individual toxin chains to the host cytosol. Released effector toxin chains cause disassembly of the actin cytoskeleton and enhance V.vulnificus colonization of the small intestine, possibly by facilitating evasion of phagocytic cells"}],"commentType":"FUNCTION","molecule":"Cysteine protease domain-containing toxin F3"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Actin-directed toxin that catalyzes the covalent cross-linking of host cytoplasmic monomeric actin. Mediates the cross-link between 'Lys-50' of one monomer and 'Glu-270' of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners. Acts as an acid--amino-acid ligase that transfers the gamma-phosphoryl group of ATP to the 'Glu-270' actin residue, resulting in the formation of an activated acyl phosphate intermediate. This intermediate is further hydrolyzed and the energy of hydrolysis is utilized for the formation of the amide bond between actin subunits"}],"commentType":"FUNCTION","molecule":"Actin cross-linking toxin F1"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"N-epsilon-fatty acyltransferase that mediates lysine-palmitoylation of host Rho GTPase proteins, with a strong preference for host Rac1. After delivery to the host cytosol, localizes to the host cell membrane where it palmitoylates host Rho GTPase proteins, resulting in loss of all active GTP-bound Rho and subsequent actin depolymerization. Prenylation of host Rac1 at the C-terminus is required for lysine-palmitoylation"}],"commentType":"FUNCTION","molecule":"N-epsilon-fatty acyltransferase F2"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Indirectly activates the small GTPase CDC42"}],"commentType":"FUNCTION","molecule":"ABH effector region toxin F5"},{"commentType":"CATALYTIC ACTIVITY","molecule":"N-epsilon-fatty acyltransferase F2","reaction":{"name":"L-lysyl-/S-(2E,6E,10E)-geranylgeranyl-L-cysteinyl-[protein] + hexadecanoyl-CoA = N(6)-hexadecanoyl-L-lysyl-/S-(2E,6E,10E)-geranylgeranyl-L-cysteinyl-[protein] + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59768"},{"database":"Rhea","id":"RHEA-COMP:17936"},{"database":"Rhea","id":"RHEA-COMP:17953"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29969"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57379"},{"database":"ChEBI","id":"CHEBI:86021"},{"database":"ChEBI","id":"CHEBI:138936"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:59769"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}]}]},{"commentType":"CATALYTIC ACTIVITY","molecule":"N-epsilon-fatty acyltransferase F2","reaction":{"name":"L-lysyl-/S-(2E,6E,10E)-geranylgeranyl-L-cysteinyl-[protein] + dodecanoyl-CoA = N(6)-dodecanoyl-L-lysyl-/S-(2E,6E,10E)-geranylgeranyl-L-cysteinyl-[protein] + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59796"},{"database":"Rhea","id":"RHEA-COMP:17936"},{"database":"Rhea","id":"RHEA-COMP:17954"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29969"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57375"},{"database":"ChEBI","id":"CHEBI:86021"},{"database":"ChEBI","id":"CHEBI:143221"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:59797"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}]}]},{"commentType":"CATALYTIC ACTIVITY","molecule":"N-epsilon-fatty acyltransferase F2","reaction":{"name":"L-lysyl-/S-(2E,6E,10E)-geranylgeranyl-L-cysteinyl-[protein] + decanoyl-CoA = N(6)-decanoyl-L-lysyl-/S-(2E,6E,10E)-geranylgeranyl-L-cysteinyl-[protein] + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59800"},{"database":"Rhea","id":"RHEA-COMP:17936"},{"database":"Rhea","id":"RHEA-COMP:17955"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29969"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:61430"},{"database":"ChEBI","id":"CHEBI:86021"},{"database":"ChEBI","id":"CHEBI:143222"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:59801"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A0H3AIG7"}],"value":"Binds 2 Mg(2+) ions per subunit. Mg(2+) is required for actin cross-linking activity. Can also use Mn(2+) ions instead of Mg(2+)."}]}},{"commentType":"SUBCELLULAR LOCATION","molecule":"Multifunctional-autoprocessing repeats-in-toxin","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Secreted via the type I secretion system"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Secreted","id":"SL-0243"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Host cytoplasm, host cytosol","id":"SL-0384"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"N-epsilon-fatty acyltransferase F2","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Targeted to the host cell membrane via the membrane localization region (MLD)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Host cell membrane","id":"SL-0375"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"Actin cross-linking toxin F1","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"value":"Host cytoplasm, host cytosol","id":"SL-0384"}}]}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Chain","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":5206,"modifier":"EXACT"}},"description":"Multifunctional-autoprocessing repeats-in-toxin","featureId":"PRO_0000455658"},{"type":"Chain","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":1958,"modifier":"EXACT"}},"description":"Actin cross-linking toxin F1","featureId":"PRO_0000455659"},{"type":"Chain","location":{"start":{"value":2296,"modifier":"EXACT"},"end":{"value":2901,"modifier":"EXACT"}},"description":"N-epsilon-fatty acyltransferase F2","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"featureId":"PRO_0000455660"},{"type":"Chain","location":{"start":{"value":2902,"modifier":"EXACT"},"end":{"value":3400,"modifier":"EXACT"}},"description":"ABH effector region toxin F5","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"featureId":"PRO_0000455661"},{"type":"Chain","location":{"start":{"value":4091,"modifier":"EXACT"},"end":{"value":5206,"modifier":"EXACT"}},"description":"Cysteine protease domain-containing toxin F3","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"featureId":"PRO_0000455662"},{"type":"Repeat","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"RtxA 1","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"RtxA 2","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":141,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"RtxA 3","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"RtxA 4","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":187,"modifier":"EXACT"},"end":{"value":204,"modifier":"EXACT"}},"description":"RtxA 5","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"RtxA 6","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":255,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"RtxA 7","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":291,"modifier":"EXACT"}},"description":"RtxA 8","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":584,"modifier":"EXACT"},"end":{"value":601,"modifier":"EXACT"}},"description":"RtxA 9","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":604,"modifier":"EXACT"},"end":{"value":620,"modifier":"EXACT"}},"description":"RtxA 10","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":624,"modifier":"EXACT"},"end":{"value":641,"modifier":"EXACT"}},"description":"RtxA 11","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":644,"modifier":"EXACT"},"end":{"value":658,"modifier":"EXACT"}},"description":"RtxA 12","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":741,"modifier":"EXACT"},"end":{"value":753,"modifier":"EXACT"}},"description":"RtxA 13","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":759,"modifier":"EXACT"},"end":{"value":771,"modifier":"EXACT"}},"description":"RtxA 14","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":782,"modifier":"EXACT"},"end":{"value":798,"modifier":"EXACT"}},"description":"RtxA 15","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":801,"modifier":"EXACT"},"end":{"value":816,"modifier":"EXACT"}},"description":"RtxA 16","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":820,"modifier":"EXACT"},"end":{"value":835,"modifier":"EXACT"}},"description":"RtxA 17","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":841,"modifier":"EXACT"},"end":{"value":855,"modifier":"EXACT"}},"description":"RtxA 18","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":858,"modifier":"EXACT"},"end":{"value":875,"modifier":"EXACT"}},"description":"RtxA 19","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":877,"modifier":"EXACT"},"end":{"value":891,"modifier":"EXACT"}},"description":"RtxA 20","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":896,"modifier":"EXACT"},"end":{"value":910,"modifier":"EXACT"}},"description":"RtxA 21","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":915,"modifier":"EXACT"},"end":{"value":932,"modifier":"EXACT"}},"description":"RtxA 22","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":934,"modifier":"EXACT"},"end":{"value":950,"modifier":"EXACT"}},"description":"RtxA 23","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":972,"modifier":"EXACT"},"end":{"value":984,"modifier":"EXACT"}},"description":"RtxA 24","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":991,"modifier":"EXACT"},"end":{"value":1006,"modifier":"EXACT"}},"description":"RtxA 25","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1031,"modifier":"EXACT"},"end":{"value":1043,"modifier":"EXACT"}},"description":"RtxA 26","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1067,"modifier":"EXACT"},"end":{"value":1079,"modifier":"EXACT"}},"description":"RtxA 27","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1087,"modifier":"EXACT"},"end":{"value":1102,"modifier":"EXACT"}},"description":"RtxA 28","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1110,"modifier":"EXACT"},"end":{"value":1122,"modifier":"EXACT"}},"description":"RtxA 29","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1125,"modifier":"EXACT"},"end":{"value":1142,"modifier":"EXACT"}},"description":"RtxA 30","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1145,"modifier":"EXACT"},"end":{"value":1159,"modifier":"EXACT"}},"description":"RtxA 31","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1163,"modifier":"EXACT"},"end":{"value":1179,"modifier":"EXACT"}},"description":"RtxA 32","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1184,"modifier":"EXACT"},"end":{"value":1199,"modifier":"EXACT"}},"description":"RtxA 33","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1201,"modifier":"EXACT"},"end":{"value":1217,"modifier":"EXACT"}},"description":"RtxA 34","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1220,"modifier":"EXACT"},"end":{"value":1236,"modifier":"EXACT"}},"description":"RtxA 35","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1242,"modifier":"EXACT"},"end":{"value":1256,"modifier":"EXACT"}},"description":"RtxA 36","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1258,"modifier":"EXACT"},"end":{"value":1275,"modifier":"EXACT"}},"description":"RtxA 37","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1296,"modifier":"EXACT"},"end":{"value":1313,"modifier":"EXACT"}},"description":"RtxA 38","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":1315,"modifier":"EXACT"},"end":{"value":1332,"modifier":"EXACT"}},"description":"RtxA 39","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Domain","location":{"start":{"value":4111,"modifier":"EXACT"},"end":{"value":4295,"modifier":"EXACT"}},"description":"Peptidase C80","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01107"}]},{"type":"Region","location":{"start":{"value":1606,"modifier":"EXACT"},"end":{"value":1682,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1738,"modifier":"EXACT"},"end":{"value":1895,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":2377,"modifier":"EXACT"},"end":{"value":2461,"modifier":"EXACT"}},"description":"Membrane localization region (MLD)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}]},{"type":"Region","location":{"start":{"value":2537,"modifier":"EXACT"},"end":{"value":2901,"modifier":"EXACT"}},"description":"Rho inactivation domain (RID)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}]},{"type":"Region","location":{"start":{"value":2998,"modifier":"EXACT"},"end":{"value":3113,"modifier":"EXACT"}},"description":"ABH effector region","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}]},{"type":"Region","location":{"start":{"value":4333,"modifier":"EXACT"},"end":{"value":4362,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":4738,"modifier":"EXACT"},"end":{"value":4779,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1606,"modifier":"EXACT"},"end":{"value":1626,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1627,"modifier":"EXACT"},"end":{"value":1646,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1652,"modifier":"EXACT"},"end":{"value":1671,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1778,"modifier":"EXACT"},"end":{"value":1805,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1825,"modifier":"EXACT"},"end":{"value":1834,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1835,"modifier":"EXACT"},"end":{"value":1849,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1870,"modifier":"EXACT"},"end":{"value":1880,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":4750,"modifier":"EXACT"},"end":{"value":4762,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":4181,"modifier":"EXACT"},"end":{"value":4181,"modifier":"EXACT"}},"description":"For cysteine protease activity","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01107"}]},{"type":"Active site","location":{"start":{"value":4230,"modifier":"EXACT"},"end":{"value":4230,"modifier":"EXACT"}},"description":"Nucleophile; for cysteine protease activity","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01107"}]},{"type":"Binding site","location":{"start":{"value":4117,"modifier":"EXACT"},"end":{"value":4119,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58130"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"ligand":{"name":"1D-myo-inositol hexakisphosphate","id":"ChEBI:CHEBI:58130"}},{"type":"Binding site","location":{"start":{"value":4144,"modifier":"EXACT"},"end":{"value":4145,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58130"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"ligand":{"name":"1D-myo-inositol hexakisphosphate","id":"ChEBI:CHEBI:58130"}},{"type":"Binding site","location":{"start":{"value":4175,"modifier":"EXACT"},"end":{"value":4175,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58130"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"ligand":{"name":"1D-myo-inositol hexakisphosphate","id":"ChEBI:CHEBI:58130"}},{"type":"Binding site","location":{"start":{"value":4226,"modifier":"EXACT"},"end":{"value":4226,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58130"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"ligand":{"name":"1D-myo-inositol hexakisphosphate","id":"ChEBI:CHEBI:58130"}},{"type":"Binding site","location":{"start":{"value":4259,"modifier":"EXACT"},"end":{"value":4261,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58130"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"ligand":{"name":"1D-myo-inositol hexakisphosphate","id":"ChEBI:CHEBI:58130"}},{"type":"Binding site","location":{"start":{"value":4272,"modifier":"EXACT"},"end":{"value":4273,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58130"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"ligand":{"name":"1D-myo-inositol hexakisphosphate","id":"ChEBI:CHEBI:58130"}},{"type":"Binding site","location":{"start":{"value":4285,"modifier":"EXACT"},"end":{"value":4285,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58130"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"ligand":{"name":"1D-myo-inositol hexakisphosphate","id":"ChEBI:CHEBI:58130"}},{"type":"Binding site","location":{"start":{"value":4290,"modifier":"EXACT"},"end":{"value":4290,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58130"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9KS12"}],"ligand":{"name":"1D-myo-inositol hexakisphosphate","id":"ChEBI:CHEBI:58130"}},{"type":"Helix","location":{"start":{"value":1969,"modifier":"EXACT"},"end":{"value":1984,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Turn","location":{"start":{"value":1985,"modifier":"EXACT"},"end":{"value":1988,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":1993,"modifier":"EXACT"},"end":{"value":1995,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8K9Z"}]},{"type":"Helix","location":{"start":{"value":2010,"modifier":"EXACT"},"end":{"value":2025,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2027,"modifier":"EXACT"},"end":{"value":2029,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Turn","location":{"start":{"value":2033,"modifier":"EXACT"},"end":{"value":2035,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2036,"modifier":"EXACT"},"end":{"value":2048,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2052,"modifier":"EXACT"},"end":{"value":2063,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Turn","location":{"start":{"value":2064,"modifier":"EXACT"},"end":{"value":2066,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2074,"modifier":"EXACT"},"end":{"value":2076,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2077,"modifier":"EXACT"},"end":{"value":2079,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2081,"modifier":"EXACT"},"end":{"value":2085,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2087,"modifier":"EXACT"},"end":{"value":2090,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2091,"modifier":"EXACT"},"end":{"value":2097,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2099,"modifier":"EXACT"},"end":{"value":2101,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA1"}]},{"type":"Beta strand","location":{"start":{"value":2103,"modifier":"EXACT"},"end":{"value":2105,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2107,"modifier":"EXACT"},"end":{"value":2109,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2111,"modifier":"EXACT"},"end":{"value":2129,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2133,"modifier":"EXACT"},"end":{"value":2135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA1"}]},{"type":"Helix","location":{"start":{"value":2136,"modifier":"EXACT"},"end":{"value":2161,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2166,"modifier":"EXACT"},"end":{"value":2172,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2175,"modifier":"EXACT"},"end":{"value":2180,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2182,"modifier":"EXACT"},"end":{"value":2186,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2187,"modifier":"EXACT"},"end":{"value":2195,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2198,"modifier":"EXACT"},"end":{"value":2205,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2209,"modifier":"EXACT"},"end":{"value":2212,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2213,"modifier":"EXACT"},"end":{"value":2215,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA2"}]},{"type":"Helix","location":{"start":{"value":2217,"modifier":"EXACT"},"end":{"value":2219,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2220,"modifier":"EXACT"},"end":{"value":2222,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2226,"modifier":"EXACT"},"end":{"value":2228,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8K9Z"}]},{"type":"Beta strand","location":{"start":{"value":2231,"modifier":"EXACT"},"end":{"value":2234,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2235,"modifier":"EXACT"},"end":{"value":2237,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2240,"modifier":"EXACT"},"end":{"value":2249,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2251,"modifier":"EXACT"},"end":{"value":2253,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2259,"modifier":"EXACT"},"end":{"value":2262,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2268,"modifier":"EXACT"},"end":{"value":2276,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Turn","location":{"start":{"value":2278,"modifier":"EXACT"},"end":{"value":2280,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2281,"modifier":"EXACT"},"end":{"value":2286,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2288,"modifier":"EXACT"},"end":{"value":2293,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2296,"modifier":"EXACT"},"end":{"value":2301,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Turn","location":{"start":{"value":2302,"modifier":"EXACT"},"end":{"value":2304,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2310,"modifier":"EXACT"},"end":{"value":2316,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2320,"modifier":"EXACT"},"end":{"value":2333,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2337,"modifier":"EXACT"},"end":{"value":2339,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2342,"modifier":"EXACT"},"end":{"value":2347,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Beta strand","location":{"start":{"value":2350,"modifier":"EXACT"},"end":{"value":2370,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8KA0"}]},{"type":"Helix","location":{"start":{"value":2377,"modifier":"EXACT"},"end":{"value":2380,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2381,"modifier":"EXACT"},"end":{"value":2387,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2397,"modifier":"EXACT"},"end":{"value":2412,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Beta strand","location":{"start":{"value":2414,"modifier":"EXACT"},"end":{"value":2416,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2422,"modifier":"EXACT"},"end":{"value":2440,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2447,"modifier":"EXACT"},"end":{"value":2465,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2480,"modifier":"EXACT"},"end":{"value":2484,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2487,"modifier":"EXACT"},"end":{"value":2490,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Beta strand","location":{"start":{"value":2510,"modifier":"EXACT"},"end":{"value":2512,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2513,"modifier":"EXACT"},"end":{"value":2518,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2525,"modifier":"EXACT"},"end":{"value":2548,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2552,"modifier":"EXACT"},"end":{"value":2555,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Beta strand","location":{"start":{"value":2559,"modifier":"EXACT"},"end":{"value":2561,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2564,"modifier":"EXACT"},"end":{"value":2578,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Beta strand","location":{"start":{"value":2583,"modifier":"EXACT"},"end":{"value":2591,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Beta strand","location":{"start":{"value":2598,"modifier":"EXACT"},"end":{"value":2603,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2613,"modifier":"EXACT"},"end":{"value":2621,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Beta strand","location":{"start":{"value":2623,"modifier":"EXACT"},"end":{"value":2627,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2629,"modifier":"EXACT"},"end":{"value":2638,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Turn","location":{"start":{"value":2644,"modifier":"EXACT"},"end":{"value":2646,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2654,"modifier":"EXACT"},"end":{"value":2657,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2683,"modifier":"EXACT"},"end":{"value":2695,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2705,"modifier":"EXACT"},"end":{"value":2708,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2710,"modifier":"EXACT"},"end":{"value":2718,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2720,"modifier":"EXACT"},"end":{"value":2723,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2724,"modifier":"EXACT"},"end":{"value":2726,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2730,"modifier":"EXACT"},"end":{"value":2742,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2747,"modifier":"EXACT"},"end":{"value":2764,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2768,"modifier":"EXACT"},"end":{"value":2776,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2777,"modifier":"EXACT"},"end":{"value":2781,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2782,"modifier":"EXACT"},"end":{"value":2797,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Beta strand","location":{"start":{"value":2801,"modifier":"EXACT"},"end":{"value":2808,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2813,"modifier":"EXACT"},"end":{"value":2822,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2827,"modifier":"EXACT"},"end":{"value":2834,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2838,"modifier":"EXACT"},"end":{"value":2848,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2851,"modifier":"EXACT"},"end":{"value":2855,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2862,"modifier":"EXACT"},"end":{"value":2864,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":2868,"modifier":"EXACT"},"end":{"value":2886,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XN7"}]},{"type":"Helix","location":{"start":{"value":3595,"modifier":"EXACT"},"end":{"value":3603,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2N9W"}]},{"type":"Helix","location":{"start":{"value":3612,"modifier":"EXACT"},"end":{"value":3614,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2N9W"}]},{"type":"Helix","location":{"start":{"value":3615,"modifier":"EXACT"},"end":{"value":3622,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2N9W"}]},{"type":"Turn","location":{"start":{"value":3623,"modifier":"EXACT"},"end":{"value":3625,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2N9W"}]},{"type":"Helix","location":{"start":{"value":3629,"modifier":"EXACT"},"end":{"value":3648,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2N9W"}]},{"type":"Helix","location":{"start":{"value":3654,"modifier":"EXACT"},"end":{"value":3664,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2N9W"}]},{"type":"Helix","location":{"start":{"value":3667,"modifier":"EXACT"},"end":{"value":3669,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2N9W"}]},{"type":"Helix","location":{"start":{"value":4101,"modifier":"EXACT"},"end":{"value":4103,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4121,"modifier":"EXACT"},"end":{"value":4127,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Helix","location":{"start":{"value":4132,"modifier":"EXACT"},"end":{"value":4144,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Turn","location":{"start":{"value":4146,"modifier":"EXACT"},"end":{"value":4148,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4149,"modifier":"EXACT"},"end":{"value":4154,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4160,"modifier":"EXACT"},"end":{"value":4165,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Helix","location":{"start":{"value":4167,"modifier":"EXACT"},"end":{"value":4169,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4172,"modifier":"EXACT"},"end":{"value":4179,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4182,"modifier":"EXACT"},"end":{"value":4187,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJA"}]},{"type":"Beta strand","location":{"start":{"value":4190,"modifier":"EXACT"},"end":{"value":4193,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJA"}]},{"type":"Helix","location":{"start":{"value":4198,"modifier":"EXACT"},"end":{"value":4216,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4223,"modifier":"EXACT"},"end":{"value":4231,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4237,"modifier":"EXACT"},"end":{"value":4240,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Helix","location":{"start":{"value":4241,"modifier":"EXACT"},"end":{"value":4251,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4257,"modifier":"EXACT"},"end":{"value":4263,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4265,"modifier":"EXACT"},"end":{"value":4267,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4273,"modifier":"EXACT"},"end":{"value":4276,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4282,"modifier":"EXACT"},"end":{"value":4284,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Helix","location":{"start":{"value":4287,"modifier":"EXACT"},"end":{"value":4289,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]},{"type":"Beta strand","location":{"start":{"value":4290,"modifier":"EXACT"},"end":{"value":4293,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8YJC"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0012","category":"Molecular function","name":"Acyltransferase"},{"id":"KW-0068","category":"PTM","name":"Autocatalytic cleavage"},{"id":"KW-1032","category":"Cellular component","name":"Host cell membrane"},{"id":"KW-1035","category":"Cellular component","name":"Host cytoplasm"},{"id":"KW-1043","category":"Cellular component","name":"Host membrane"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"id":"KW-0446","category":"Ligand","name":"Lipid-binding"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0788","category":"Molecular function","name":"Thiol protease"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"29358930","citationType":"journal article","authors":["Roig F.J.","Gonzalez-Candelas F.","Sanjuan E.","Fouz B.","Feil E.J.","Llorens C.","Baker-Austin C.","Oliver J.D.","Danin-Poleg Y.","Gibas C.J.","Kashi Y.","Gulig P.A.","Morrison S.S.","Amaro C."],"citationCrossReferences":[{"database":"PubMed","id":"29358930"},{"database":"DOI","id":"10.3389/fmicb.2017.02613"}],"title":"Phylogeny of Vibrio vulnificus from the Analysis of the Core-Genome: Implications for Intra-Species Taxonomy.","publicationDate":"2018","journal":"Front. Microbiol.","firstPage":"2613","lastPage":"2613","volume":"8"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"CECT4608","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"29074776","citationType":"journal article","authors":["Zhou Y.","Huang C.","Yin L.","Wan M.","Wang X.","Li L.","Liu Y.","Wang Z.","Fu P.","Zhang N.","Chen S.","Liu X.","Shao F.","Zhu Y."],"citationCrossReferences":[{"database":"PubMed","id":"29074776"},{"database":"DOI","id":"10.1126/science.aam8659"}],"title":"Nepsilon-fatty acylation of Rho GTPases by a MARTX toxin effector.","publicationDate":"2017","journal":"Science","firstPage":"528","lastPage":"531","volume":"358"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (N-EPSILON-FATTY ACYLTRANSFERASE F2)"],"referenceComments":[{"value":"ATCC 27562 / DSM 10143 / JCM 3725 / BCRC 12905 / CCUG 13448 / KCTC 2959 / LMG 13545 / NBRC 15645 / NCIMB 2046 / WDCM 00139 / 324","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"5XN7"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"PDGH01000027","properties":[{"key":"ProteinId","value":"POB49698.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_103199790.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_PDGH01000027.1"}]},{"database":"PDB","id":"2N9W","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=3591-3669"}]},{"database":"PDB","id":"5XN7","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"A/B=2286-2901"}]},{"database":"PDB","id":"8K9Z","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.90 A"},{"key":"Chains","value":"A/C=1967-2371"}]},{"database":"PDB","id":"8KA0","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.35 A"},{"key":"Chains","value":"A/C/E/G=1959-2374"}]},{"database":"PDB","id":"8KA1","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.82 A"},{"key":"Chains","value":"A/C/E/G=1959-2374"}]},{"database":"PDB","id":"8KA2","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"3.38 A"},{"key":"Chains","value":"A/B=1959-2374"}]},{"database":"PDB","id":"8YJA","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"A/B=4090-4296"}]},{"database":"PDB","id":"8YJC","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.30 A"},{"key":"Chains","value":"A=4086-4299"}]},{"database":"EMDB","id":"EMD-37593","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A2S3R7M0","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAMQKEW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0044164","properties":[{"key":"GoTerm","value":"C:host cell cytosol"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0020002","properties":[{"key":"GoTerm","value":"C:host cell plasma membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0140772","properties":[{"key":"GoTerm","value":"F:CoA-dependent peptidyl-lysine N6-palmitoyltransferase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"CDD","id":"cd20495","properties":[{"key":"EntryName","value":"C58_PaToxP-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd20501","properties":[{"key":"EntryName","value":"C80_RtxA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd14729","properties":[{"key":"EntryName","value":"RtxA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16840","properties":[{"key":"EntryName","value":"toxin_MLD"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"1.20.140.180","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.40.50.11050","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.11550","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1820","properties":[{"key":"EntryName","value":"alpha/beta hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.260","properties":[{"key":"EntryName","value":"Galactose-binding domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR000073","properties":[{"key":"EntryName","value":"AB_hydrolase_1"}]},{"database":"InterPro","id":"IPR029058","properties":[{"key":"EntryName","value":"AB_hydrolase_fold"}]},{"database":"InterPro","id":"IPR020974","properties":[{"key":"EntryName","value":"CPD_dom"}]},{"database":"InterPro","id":"IPR038383","properties":[{"key":"EntryName","value":"CPD_dom_sf"}]},{"database":"InterPro","id":"IPR063956","properties":[{"key":"EntryName","value":"CRN52_02910-like_PED"}]},{"database":"InterPro","id":"IPR020972","properties":[{"key":"EntryName","value":"Dermonecrotic/RTX_toxin_MLD"}]},{"database":"InterPro","id":"IPR059883","properties":[{"key":"EntryName","value":"IcsB/BopA_M"}]},{"database":"InterPro","id":"IPR061305","properties":[{"key":"EntryName","value":"MARTX_Nterm"}]},{"database":"InterPro","id":"IPR049824","properties":[{"key":"EntryName","value":"RtxA-like_C80"}]},{"database":"InterPro","id":"IPR048568","properties":[{"key":"EntryName","value":"RtxA_C"}]},{"database":"InterPro","id":"IPR011509","properties":[{"key":"EntryName","value":"RtxA_toxin"}]},{"database":"InterPro","id":"IPR011049","properties":[{"key":"EntryName","value":"Serralysin-like_metalloprot_C"}]},{"database":"InterPro","id":"IPR048461","properties":[{"key":"EntryName","value":"ToxA-like_C2"}]},{"database":"NCBIfam","id":"NF012221","properties":[{"key":"EntryName","value":"MARTX_Nterm"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12277","properties":[{"key":"EntryName","value":"ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12277:SF81","properties":[{"key":"EntryName","value":"PROTEIN ABHD13"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00561","properties":[{"key":"EntryName","value":"Abhydrolase_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF27349","properties":[{"key":"EntryName","value":"IcsB_M"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF30660","properties":[{"key":"EntryName","value":"Mcf1_PED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11647","properties":[{"key":"EntryName","value":"MLD"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF11713","properties":[{"key":"EntryName","value":"Peptidase_C80"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF20899","properties":[{"key":"EntryName","value":"PMT_C2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07634","properties":[{"key":"EntryName","value":"RtxA"},{"key":"MatchStatus","value":"41"}]},{"database":"Pfam","id":"PF21735","properties":[{"key":"EntryName","value":"RtxA_C"},{"key":"MatchStatus","value":"6"}]},{"database":"SUPFAM","id":"SSF53474","properties":[{"key":"EntryName","value":"alpha/beta-Hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51120","properties":[{"key":"EntryName","value":"beta-Roll"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF159501","properties":[{"key":"EntryName","value":"EreA/ChaN-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF158842","properties":[{"key":"EntryName","value":"PMT central region-like"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51771","properties":[{"key":"EntryName","value":"CGT_MARTX_CPD"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGKPFWRSVEYFFTGNYSADDGNNSIVAIGFGGEIHAYGGDDHVTVGSIGATVYTGSGNDTVVGGSAYLRVEDTTGHLSVKGAAGYADINKSGDGNVSFAGAAGGVSIDHLGNNGDVSYGGAAAYNGITRKGLSGNVTFKGAGGYNALWHETNQGNLSFAGAGAGNKLDRTWFNRYQGSRGDVTFDGAGAANSISSRVETGNITFRGAGADNHLVRKGKVGDITLQGAGASNRIERTRQAEDVYAQTRGNIRFEGVGGYNSLYSDVAHGDIHFSGGGAYNTITRKGSGSSFDAQGMEYAKAEDIVLTAAQMHGLSIDNGNKFHAVTAVKSEREPNTYLFAIADGTYTKINKVRLYNDPETGKLKYYSEAWFKRGNHLAELARSDVSSAGGFEVNPINGGYTLANIAVEHQQSVTVHAVEKNLTEYEWVTYANGTLIDAKDVALSEAKMGGHAISTDGTTVDVQAVKSNRKPNTYVYAKVLGPYTKIVVVELANDPKTGALKYQARSWYKEGDHTANLANEDISSANGYHSMGKGGYSLSDLHYSVNAVRSTSETVADIDEYTDQTLFKPATDSGESSGDVRFNGAGGGNVIKSNVTRGNVYFNGGGIANVILHSSQFGNTEFNGGGAANVIVKSGEEGDLTFRGAGLANVLVHQSKQGKMDVYAGGAVNVLVRIGDGQYLAHLLAYGNISVHKGNGNSRVVMLGGYNTHTQIGSGNGLWLAAGGFNVMTQVGKGDVASVLAGGANVLTKVGDGDLTAGMLGGANVITHISGDNETSNTTAVALGGANILTKKGKGNTLAVMGGGANVLTHVGDGTTTGVMVGGANILTKVGNGDTTGIMLGVGNVLTHVGDGQTLGVMGAAGNIFTKVGDGTSIAVMIGAGNIFTHVGEGNAWALMGGLGNVFTKVGNGDALALMVAEANVFTHIGDGMSVALMLAKGNVATKVGNGTTLAAMVGNANIFTHVGSGSTFAAMIGQANIMTKVGNDLTAALMVGKANIYTHVGDGTSLGIFAGEVNVMTKIGNGTTLAAMFGKANIMTHVGDGLTGVLALGEANIVTKVGDDFMGVVAAAKANVVTHVGDATTAAVLAGKGNILTKVGEGTTVGLLISDIGNVMTHVGDGTTIGIAKGKANIITKVGDGLGVNVAWGQANVFTQVGDGDRYNFAKGEANIITKVGDGKEVSVVQGKANIITHVGNGDDYTGAWGKANVITKVGNGRNVVLAKGEANIVTQVGDGDSFNALWSKGNIVTKVGDGMQVTAAKGKANITTTVGDGLSVTAAYGDANINTKVGDGVSVNVAWGKYNINTKVGDGLNVAVMKGKANANIHVGDGLNINASYAQNNVAIKVGNGDFYSLAVASSNTSSNKLSALFDNIKQTLLGVGGSQAINYLVQGDEASSSGTQKGRGAIATPEITKLDGFQMEAIEEVGSDLGDSLTGSVTKVDTPDLNKMQNALDVDGSSDQTQAPNLIVNGDFEQGDRGWKSTHGVEASYSGNVYGVNGEGHGARVTELDTYTNTSLYQDLTDLTEGEVIAVSFDFAKRAGLSNNEGIEVLWNGEVVFSSSGDASAWQQKTLKLTAHAGSNRIEFKGTGHNDGLGYILDNVVAKSESSQQANAVSEHATQNQASQNALSDKERAEADRQRLEQEKQKQLDAVAGSQSQLESTDQQALGNNGQAQRDAVKEESEAVTAELTKLAQGLDVLDGQATHTGESGDQWRNDFAGGLLDGVQSQLDDAKQLANDKIAAAKQTQSDNNSKVKESVAKSEAGVAQGEQNRAGAEQDIAEAKADAETRKADAVAKSNDAKQAESDAHSAANDAQSRGDRDAMNAENKANQAQNDAKGTKQNEGDRPDREGVAGSGLSGNAHSVEGAGETGSHITTDSQTNADGRFSEGLSEQEQEALEGATNAVNRLQINAGIRGKNSGSTITSMFTETNSDSIVVPTTASQDVVRKEIRISGVNLEGLGEASHDSAESLVAARAEKVANLYRWLDTDNDVATDKYVPVPGFERVDVDVSDEVKQRMIQSMSGYIEHTDNQVPKDQAEALATLFVESTLDYDWDKRVEFLTKLESYGYSFEAPHAEKSIVSFWSGKNFKQYRDILDNAQTDGKKVVYDIDVKGNAFAIDLNKHLMRWGGLFLDPDNAEQNQLKSSIDAATFSNTGFWSSVYATGAQNDVYVIAEGGVRLGNYFWNVELPALRQLQREGLVGEIRLLDKPVSEYKDLPADQIGRRLTDAGVAVKVRFDALSHERQAELLADNPDGYKADTLVELDVKLSAIDSMLRESLPFYSLRTERNLLVQEGEEGFEVRSWPGIDGKSKTILLDNPEDAAQQKSIERFILANFDNFEQMPDELFLVDNKVLSHHDGRTRIIAQKEDGAWTYNTNVELMSVTELLDAAHVNGKVRGDSYQQVIDALTEYHASTVEHADYELESVEKLLNLRKQIEGYVLGHPDSGRVEAMNSLLNQVNSRLEEVSVLAVSEQSIKAHDSFSRLYDQLDNANLKESKHLYLDGNGDFVTKGKGNLATIDQLGGSDAVLEKVKAAVTHEYGQVVADTIFARLSANDLAKDGKGIDIAGLNKVHQAIEQHMSPVSATMYIWKPSDHSTLGHAALQIGQGRTQLEGQAAADFNKQNYVSWWPLGSKSSNIRNIFNVATEDQPDLKLRWSDFSQPAHQNDTLEHDMASEENDGFGLKDGETKLKRFIEKLNAAKGIDASYKDASEGYASVLLGNPDMLASTGIPAHVFQPFVDQWNDTSYDMMDVANRFAEELQKQAQASGDPALVEKRIDNVVRLFAERALEEIEAFKASQADEGRVFRINLEGLDVAAMQAEWNRLSNDPDARYQLLTKNCSSTVAKVLKAGGADKLIGHTWRPKFGVWTPTELFNFGQALQEAQLEIAAKKQSHQVTDVLDALSGNEKHKENVTIENDGTPPRDKESLSPLTRFLNNELYGEKDARRKIGEITQTLLDHAVENGESQKVTLKGEAGRLTGYYHQGAASSEGETSATSGKVVLFLHGSGSSAEEQASAIRNHYQKQGIDMLAVNLRGYGESDGGPSEKGLYQDARTMFNYLVNDKGIDPSNIIIHGYSMGGPIAADLARYAAQNGQAVSGLLLDRPMPSMTKAITAHEMANPAGIVGAIAKAVNGQFSVEKNLKGLPKETPILLLTDNEGLGEEGEKLRAKLAIAGYNVTGEQTFYGHEASNRLMGQYADQIVSGLFNAEQAAVEAGEVLKGLEKDFKRYGDALKPDTSVPGKSKDIRTTKDFLNGYKNDHAKEIVDGFRSDMSIKQLVDLFVKGNWSAEQKGALAWEIESRALKVTFQNKSEKYNRLFREIASAGVVDAKATEQLAPQLMLLNLSNDGFGGRCDPLSKLVLVAKQLENDGQVGVARQLLEKMYSAAAVLSNPTLYSDSEKANASKLLSSLAAIHAKNPMHDTSMKVWQEKLEGKQALTVNGVVEKITDASANGKPVLLELDAPGHAMAAWAKDSGDDRVYGFYDPNAGIVEFSSAEKFGDYLTRFFGKSDLDMAQSYKLGKNDAGEAIFNRVVVMDGNTLASYKPTFGDKTTMQGILDLPVFDATPIKKPTGGVASDLEALGDKTKVVVDLAQIFTVQELKERAKVFAKPIGASYQGILDQLDLVHQAKGRDQIAASFELNKKINDYIAEHPTSGRNQALTQLKEQVTSALFIGKMQVAQAGIDAIAQTRPELAARIFMVAIEEANGKHVGLTDMMVRWANEDPYLAPKHGYKGETPSDLGFDAKYHVDLGEHYADFKQWLETSQSNGLLSKATLDESTKTVHLGYSYQELQDLTGAESVQMAFYFLKEAAKKADPISGDSAEMILLKKFADQSYLSQLDSDRMDQIEGIYRSSHETDIDAWDRRYSGTGYDELTNKLASATGVDEQLAVLLDDRKGLLIGEVHGSDVNGLRFVNEQMDALKKQGVTVIGLEHLRSDLAQPLIDRYLATGVMSSELSAMLKTKHLDVTLFENARANGIRIVALDANSSARPNVQGTEHGLMYRAGAANNIAVEVLQNLPDGEKFVAIYGKAHLQSHKGIEGFVPGITHRLDLPALKVSDSNQFTVEQDDVSLRVVYDDVANKPKITFKDSLSGANTALHNQNVNDWERVVVTPTADGGESRFDGQIIVQMENDDVVAKAAANLAGKHPESSVVVQIDSDGNYRVVYGDPSKLDGKLRWQLVGHGRDDSESNNTRLSGYSADELAVKLAKFQQSFNQAENINNKPDHISIVGCSLVSDDKQKGFGHQFINAMDANGLRVDVSVRSSELAVDEAGRKHTKDANGDWVQKAENNKVSLSWDEQGEVVAKDERIRNGIAEGDIDLSRIGVSDVDEPARGAIGDNNDVFDAPEKRKAETETSSSSANNKLSYSGNIQVNVGDGEFTAVNWGTSNVGIKVGTGGFKSLAFGDNNVMVHIGNGESKHSFDIGGYQALEGAQMFIGNRNVSFNLGRSNDLIVMMDKSIPTPPLVNPFDGAARISGVLQSIATSGEGQDWLAAQEQQWTLSGAKKFVKDMSGLDQSSSVDYTSLVELDSQNERSSRGLKHDAEAALNKQYNQWLSGNSDSDTSKLSRADKLRQANEKLAFNFAVGGQGADIQVTTGNWNFMFGDNIQSILDTNLGSLFGLMTQQFSATGQAKTTFTYTPEDLPRQLKNKLLGQLAGVGAETTLADIFGVDYTASGQIVSRNGEAVDGVAILKEMLEVIGEFSGDQLQAFVDPAKLLDSLKSGINMGADGIKSFAETHGLKEKAPEEEEDNSSVSVNGASVNSAQGATVADGSTETAETPDRAFGFNSLNLPNLFATIFSQDKQKEMKSLVENLKENLTADLLNMKEKTFDFLRNSGHLQGDGDINISLGNYNFNWGGDGKDLGAYLGDNNNFWGGRGDDVFYATGTSNIFTGGEGNDMGVLMGRENMMFGGDGNDTAVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTSGNFNRVDTGDDQDYSVTIGNNNQVELGAGNDFANVFGNYNRINASAGNDVVKLMGYHAVLNGGEGEDHLIAAAISKFSQFNGGEGRDLMVLGGYQNTFKGGTDVDSFVVSGDVIDNLVEDIRSEDNIVFNGIDWQKLWFERSGYDLKLSILRDPASDSDQAKFEHIGSVTFSDYFNGNRAQVIIAMGEKDATGEREYTTLSESAIDALVQAMSGFDPQAGDNGFMDNLDSKSRVAITTAWADVVHKKGITV","length":5206,"molWeight":556341,"crc64":"85BB805918FD1C5A","md5":"B4F6B6DEA876F3DCB324DA8B6BE89BF5"},"extraAttributes":{"countByCommentType":{"FUNCTION":5,"CATALYTIC ACTIVITY":3,"COFACTOR":1,"SUBCELLULAR LOCATION":3},"countByFeatureType":{"Signal":1,"Chain":5,"Repeat":39,"Domain":1,"Region":7,"Compositional bias":8,"Active site":2,"Binding site":8,"Helix":62,"Turn":8,"Beta strand":41},"uniParcId":"UPI000CD32DF6"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q04786","uniProtkbId":"HEX_VIBVL","entryAudit":{"firstPublicDate":"1995-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1995-11-01","entryVersion":121,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Beta-hexosaminidase"},"ecNumbers":[{"value":"3.2.1.52"}]},"alternativeNames":[{"fullName":{"value":"Beta-N-acetylhexosaminidase"}},{"fullName":{"value":"Chitobiase"}},{"fullName":{"value":"N-acetyl-beta-glucosaminidase"}}]},"genes":[{"geneName":{"value":"hex"}}],"comments":[{"texts":[{"value":"Hydrolysis of terminal, non-reducing N-acetyl-beta-D-glucosamine residues in chitobiose and higher analogs, and in glycoproteins"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.","ecNumber":"3.2.1.52"}},{"texts":[{"value":"Glycan degradation; chitin degradation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyl hydrolase 20 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":847,"modifier":"EXACT"}},"description":"Beta-hexosaminidase","featureId":"PRO_0000215378"},{"type":"Active site","location":{"start":{"value":519,"modifier":"EXACT"},"end":{"value":519,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":40,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":377,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":484,"modifier":"EXACT"},"end":{"value":530,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]}],"keywords":[{"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"id":"KW-0146","category":"Biological process","name":"Chitin degradation"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0326","category":"Molecular function","name":"Glycosidase"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0624","category":"Biological process","name":"Polysaccharide degradation"}],"references":[{"referenceNumber":1,"citation":{"id":"8341694","citationType":"journal article","authors":["Somerville C.C.","Colwell R.R."],"citationCrossReferences":[{"database":"PubMed","id":"8341694"},{"database":"DOI","id":"10.1073/pnas.90.14.6751"}],"title":"Sequence analysis of the beta-N-acetylhexosaminidase gene of Vibrio vulnificus: evidence for a common evolutionary origin of hexosaminidases.","publicationDate":"1993","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"6751","lastPage":"6755","volume":"90"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 27562 / DSM 10143 / JCM 3725 / BCRC 12905 / CCUG 13448 / KCTC 2959 / LMG 13545 / NBRC 15645 / NCIMB 2046 / WDCM 00139 / 324","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L04544","properties":[{"key":"ProteinId","value":"AAA27527.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A48228","properties":[{"key":"EntryName","value":"A48228"}]},{"database":"AlphaFoldDB","id":"Q04786","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q04786","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00349","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004563","properties":[{"key":"GoTerm","value":"F:beta-N-acetylhexosaminidase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0030247","properties":[{"key":"GoTerm","value":"F:polysaccharide binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005975","properties":[{"key":"GoTerm","value":"P:carbohydrate metabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006032","properties":[{"key":"GoTerm","value":"P:chitin catabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"GO","id":"GO:0030203","properties":[{"key":"GoTerm","value":"P:glycosaminoglycan metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd02847","properties":[{"key":"EntryName","value":"E_set_Chitobiase_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd06569","properties":[{"key":"EntryName","value":"GH20_Sm-chitobiase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.290","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.379.10","properties":[{"key":"EntryName","value":"Chitobiase/beta-hexosaminidase domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.80","properties":[{"key":"EntryName","value":"Glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.10","properties":[{"key":"EntryName","value":"Immunoglobulins"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008965","properties":[{"key":"EntryName","value":"CBM2/CBM3_carb-bd_dom_sf"}]},{"database":"InterPro","id":"IPR012291","properties":[{"key":"EntryName","value":"CBM2_carb-bd_dom_sf"}]},{"database":"InterPro","id":"IPR004866","properties":[{"key":"EntryName","value":"CHB/HEX_N_dom"}]},{"database":"InterPro","id":"IPR004867","properties":[{"key":"EntryName","value":"CHB_C_dom"}]},{"database":"InterPro","id":"IPR025705","properties":[{"key":"EntryName","value":"GH20"}]},{"database":"InterPro","id":"IPR015883","properties":[{"key":"EntryName","value":"GH20_cat"}]},{"database":"InterPro","id":"IPR017853","properties":[{"key":"EntryName","value":"GH_hydrolase_sf"}]},{"database":"InterPro","id":"IPR029018","properties":[{"key":"EntryName","value":"Hex-like_dom2"}]},{"database":"InterPro","id":"IPR015882","properties":[{"key":"EntryName","value":"HEX_bac_N"}]},{"database":"InterPro","id":"IPR013783","properties":[{"key":"EntryName","value":"Ig-like_fold"}]},{"database":"InterPro","id":"IPR014756","properties":[{"key":"EntryName","value":"Ig_E-set"}]},{"database":"PANTHER","id":"PTHR22600","properties":[{"key":"EntryName","value":"BETA-HEXOSAMINIDASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR22600:SF57","properties":[{"key":"EntryName","value":"BETA-N-ACETYLHEXOSAMINIDASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03173","properties":[{"key":"EntryName","value":"CHB_HEX"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03174","properties":[{"key":"EntryName","value":"CHB_HEX_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00728","properties":[{"key":"EntryName","value":"Glyco_hydro_20"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02838","properties":[{"key":"EntryName","value":"Glyco_hydro_20b"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00738","properties":[{"key":"EntryName","value":"GLHYDRLASE20"}]},{"database":"SMART","id":"SM01081","properties":[{"key":"EntryName","value":"CHB_HEX"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51445","properties":[{"key":"EntryName","value":"(Trans)glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55545","properties":[{"key":"EntryName","value":"beta-N-acetylhexosaminidase-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF49384","properties":[{"key":"EntryName","value":"Carbohydrate-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81296","properties":[{"key":"EntryName","value":"E set domains"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MASDIDQKDVDYAAKNLKLTTSLVANKPKDCPPEAPWGACYRVEINLENTGSKSLNENVEIYFSSIHRTLGSKSEEFKVEHINGDLHKITTTEKFKGLKGGKTKSFQVDFMNWIVSNSDFMPNYYVASEHLEGRNILNTVPIDAVHITEEVSGFTTGIKHTPNQLKRTANDLLPAATATTRYEQYSKVKDLGADAVSAHILPTPLETSVHEGSLNIAQGINIVSDALPADQVEALNFRFETLGVNTGTGVPVNVTIKADSSKKSGSYTLDVTSSGIRIVGVDKAGAFYGVQSLAGLVTVGKDTINQVSINDEPRLDYRGMHMDVSRNFHSKELVFRFLDQMAAYKMNKFHFHLADDEGWRLEINGLPELTQVGAHRCHDVEQNKCMMPQLGSGAELPNNGSGYYTREDYKEILAYASARNIQVIPSMDMPGHSLAAVKSMEARYRKFMAEGDVVKAEMYLLSDPNDTTQYYSIQHYQDNTINPCMESSFVFMDKVIDEINKLHKEGGQPLTDYHIGADETAGAWGDSPECRKMFVAPESGVKNAKDINGYFINRISHILDAKGLTLGAWNDGLSHKALDASSLAGNPPKAWVWGTMFWGGVDQYNSFANKGYDVVVTPPDAYYFDMPYENDPEERGYYWATRFNDTKKVFSFMPENVPANVEWMTDRMGAKISATTGEKTHDFLGVQGALWSETIRTDAQVEYMVLPRMIAVAERGWHKASWEEEHKEGITYTSNVDGHEGTTHLNDNIATRDADWAHFSNILGYKEMPKLDKAGITYRLPVLGAVIKNNILDVVTEFHGVAIQYSLDGKTWHKYDDTKKPQVSTKALVRSVSTNGRTGRAVEVLAK","length":847,"molWeight":94277,"crc64":"3C4405EFA34F3D14","md5":"642A2F58475C736F6B023480F3CAE60F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1,"Disulfide bond":3},"uniParcId":"UPI000012C669"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A2S3R8T2","uniProtkbId":"A0A2S3R8T2_VIBVL","entryAudit":{"firstPublicDate":"2018-07-18","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2018-07-18","entryVersion":25,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB50118.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"1,4-alpha-glucan branching enzyme GlgB"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"2.4.1.18"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"Alpha-(1->4)-glucan branching enzyme"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"Glycogen branching enzyme"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"BE"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"glgB"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB50118.1"}],"value":"CRN52_00655"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00002953"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.","ecNumber":"2.4.1.18","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000826"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004964"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"Glycan biosynthesis; glycogen biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009000"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"value":"Belongs to the glycosyl hydrolase 13 family. GlgB subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB50118.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":608,"modifier":"EXACT"}},"description":"Glycosyl hydrolase family 13 catalytic","evidences":[{"evidenceCode":"ECO:0000259","source":"SMART","id":"SM00642"}]},{"type":"Active site","location":{"start":{"value":420,"modifier":"EXACT"},"end":{"value":420,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000463-1"}]},{"type":"Active site","location":{"start":{"value":473,"modifier":"EXACT"},"end":{"value":473,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000463-1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9U77"}],"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023277"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023056"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"id":"KW-0320","category":"Biological process","name":"Glycogen biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022600"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"id":"KW-0321","category":"Biological process","name":"Glycogen metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022676"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00685"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"29358930","citationType":"journal article","authors":["Roig F.J.","Gonzalez-Candelas F.","Sanjuan E.","Fouz B.","Feil E.J.","Llorens C.","Baker-Austin C.","Oliver J.D.","Danin-Poleg Y.","Gibas C.J.","Kashi Y.","Gulig P.A.","Morrison S.S.","Amaro C."],"citationCrossReferences":[{"database":"PubMed","id":"29358930"}],"title":"Phylogeny of Vibrio vulnificus from the Analysis of the Core-Genome: Implications for Intra-Species Taxonomy.","publicationDate":"2018","journal":"Front. Microbiol.","firstPage":"2613","lastPage":"2613","volume":"8"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB50118.1"}],"value":"CECT4608","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB50118.1"}]},{"referenceNumber":2,"citation":{"id":"CI-43R0MDRB6NG4G","citationType":"submission","authors":["An Y.","Lee S.J.","Park J.T.","Woo E.J."],"title":"Structural characterization of glycogen branching enzyme (VvGBE) from Vibrio vulnificus.","publicationDate":"MAR-2025","submissionDatabase":"PDB data bank"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS)"],"evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9U77"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"PDGH01000008","properties":[{"key":"ProteinId","value":"POB50118.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PDB","id":"9U77","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"A/B=1-739"}]},{"database":"AlphaFoldDB","id":"A0A2S3R8T2","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A2S3R8T2","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AADNTPW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00164","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003844","properties":[{"key":"GoTerm","value":"F:1,4-alpha-glucan branching enzyme activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0043169","properties":[{"key":"GoTerm","value":"F:cation binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004553","properties":[{"key":"GoTerm","value":"F:hydrolase activity, hydrolyzing O-glycosyl compounds"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005978","properties":[{"key":"GoTerm","value":"P:glycogen biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd11322","properties":[{"key":"EntryName","value":"AmyAc_Glg_BE"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd02855","properties":[{"key":"EntryName","value":"E_set_GBE_prok_N"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.60.40.1180:FF:000002","properties":[{"key":"EntryName","value":"1,4-alpha-glucan branching enzyme GlgB"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.80:FF:000003","properties":[{"key":"EntryName","value":"1,4-alpha-glucan branching enzyme GlgB"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.80","properties":[{"key":"EntryName","value":"Glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.1180","properties":[{"key":"EntryName","value":"Golgi alpha-mannosidase II"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.10","properties":[{"key":"EntryName","value":"Immunoglobulins"},{"key":"MatchStatus","value":"2"}]},{"database":"HAMAP","id":"MF_00685","properties":[{"key":"EntryName","value":"GlgB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006048","properties":[{"key":"EntryName","value":"A-amylase/branching_C"}]},{"database":"InterPro","id":"IPR037439","properties":[{"key":"EntryName","value":"Branching_enzy"}]},{"database":"InterPro","id":"IPR006047","properties":[{"key":"EntryName","value":"GH13_cat_dom"}]},{"database":"InterPro","id":"IPR017853","properties":[{"key":"EntryName","value":"GH_hydrolase_sf"}]},{"database":"InterPro","id":"IPR006407","properties":[{"key":"EntryName","value":"GlgB"}]},{"database":"InterPro","id":"IPR054169","properties":[{"key":"EntryName","value":"GlgB_N"}]},{"database":"InterPro","id":"IPR044143","properties":[{"key":"EntryName","value":"GlgB_N_E_set_prok"}]},{"database":"InterPro","id":"IPR004193","properties":[{"key":"EntryName","value":"Glyco_hydro_13_N"}]},{"database":"InterPro","id":"IPR013780","properties":[{"key":"EntryName","value":"Glyco_hydro_b"}]},{"database":"InterPro","id":"IPR013783","properties":[{"key":"EntryName","value":"Ig-like_fold"}]},{"database":"InterPro","id":"IPR014756","properties":[{"key":"EntryName","value":"Ig_E-set"}]},{"database":"NCBIfam","id":"TIGR01515","properties":[{"key":"EntryName","value":"branching_enzym"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF003811","properties":[{"key":"EntryName","value":"glgB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF008967","properties":[{"key":"EntryName","value":"glgB"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43651","properties":[{"key":"EntryName","value":"1,4-ALPHA-GLUCAN-BRANCHING ENZYME"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43651:SF3","properties":[{"key":"EntryName","value":"1,4-ALPHA-GLUCAN-BRANCHING ENZYME"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00128","properties":[{"key":"EntryName","value":"Alpha-amylase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02806","properties":[{"key":"EntryName","value":"Alpha-amylase_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02922","properties":[{"key":"EntryName","value":"CBM_48"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22019","properties":[{"key":"EntryName","value":"GlgB_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000463","properties":[{"key":"EntryName","value":"GlgB"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00642","properties":[{"key":"EntryName","value":"Aamy"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51445","properties":[{"key":"EntryName","value":"(Trans)glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81296","properties":[{"key":"EntryName","value":"E set domains"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF51011","properties":[{"key":"EntryName","value":"Glycosyl hydrolase domain"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFTTLAVWRGKKGLKKLVKSKVNQMFEKLSQAACSEPFAFLGPFIDPTQGALRVWMPGATGVALVLEGQPRIALEREKESAFILKADLNLHLTHYQLAIDWNGVEQLVDDPYQYHGIYAEYDDLHTPKTMYQHMGSQFMTLERDGKSISGIRFLVYAPHATAVSLVGSFNDWDGRRHPMQRLDYGIWGLFIPGLTEGVSYKFEMKGPKGEGLPHKADPWGFYAEQYPSFASVTYDHARYQWQDAQWQTRPVTEKRKEALSFYELHAGSWKRNEQGEFLNYRELAAELVPYLVDMGYTHVELMPVSEHPFYGSWGYQPVGLFAPTSRYGSPDDFKFFVDACHQAGIGVVLDWVPAHFPSDDHGLANFDGTPLFHDPDPRRGWHQDWNSFIYDLGREQVRRFLVSNALYWFEQFHIDGIRVDAVASMLYLDYSRSHGQWIPNMDGGNENYDAIATLKWMNEEVYKYFPNAMTIAEESTAFPGVSAPTFMGGLGFGFKWNMGWMHDSLSYIKEEPVHRKYHHNTLTFPLVYAHSENYVLSLSHDEVVYGKGSIHNKMPGDEWQQTANLRAYFGYMYGQPGKKLNFMGAEIGQTAEWNHDDQLQWFLLDFPRHQGVQALTRDLNHLYRNEAALHDQDCIPAGFEWRLQDAAEQSIIAHERISEAGERILVVSNFTPVPRDEFRLGVPNKGRYQLLLNTDDSKYAGSGYEVVVDAKSEAVVSEDLAQSIVLRLPPLSTLFYKLV","length":739,"molWeight":84643,"crc64":"C62B85D942BF8DA6","md5":"D8635539EE8442F0BFC0F14A2F3BD623"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Active site":2},"uniParcId":"UPI000C7E73CF"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"C1IWS9","uniProtkbId":"C1IWS9_VIBVL","entryAudit":{"firstPublicDate":"2009-05-26","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2009-05-26","entryVersion":66,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACK76428.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"value":"4-hydroxy-4-methyl-2-oxoglutarate aldolase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"value":"HMG aldolase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"value":"4.1.1.112"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"value":"4.1.3.17"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"value":"Oxaloacetate decarboxylase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACK76428.1"}],"value":"rraAV2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00025046"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"value":"Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22748"},{"database":"ChEBI","id":"CHEBI:15361"},{"database":"ChEBI","id":"CHEBI:58276"}],"ecNumber":"4.1.3.17","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001342"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"oxaloacetate + H(+) = pyruvate + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15641"},{"database":"ChEBI","id":"CHEBI:15361"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16452"},{"database":"ChEBI","id":"CHEBI:16526"}],"ecNumber":"4.1.1.112","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047973"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR605493-1"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"a divalent metal cation","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001968"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:60240"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011233"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"value":"Homotrimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008621"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"value":"Belongs to the class II aldolase/RraA-like family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Binding site","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR605493-1"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR605493-1"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR605493-1"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004338"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR605493-1"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR605493-1"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"19194749","citationType":"journal article","authors":["Lee M.","Yeom J.H.","Sim S.H.","Ahn S.","Lee K."],"citationCrossReferences":[{"database":"PubMed","id":"19194749"},{"database":"DOI","id":"10.1007/s00284-009-9360-8"}],"title":"Effects of Escherichia coli RraA orthologs of Vibrio vulnificus on the ribonucleolytic activity of RNase E in vivo.","publicationDate":"2009","journal":"Curr. Microbiol.","firstPage":"349","lastPage":"353","volume":"58"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACK76428.1"}],"value":"MO6-24/O","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACK76428.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"EU918606","properties":[{"key":"ProteinId","value":"ACK76428.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"C1IWS9","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAPWRQF","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0047443","properties":[{"key":"GoTerm","value":"F:4-hydroxy-4-methyl-2-oxoglutarate aldolase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0008948","properties":[{"key":"GoTerm","value":"F:oxaloacetate decarboxylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0008428","properties":[{"key":"GoTerm","value":"F:ribonuclease inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0051252","properties":[{"key":"GoTerm","value":"P:regulation of RNA metabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd16841","properties":[{"key":"EntryName","value":"RraA_family"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.50.30.40","properties":[{"key":"EntryName","value":"Ribonuclease E inhibitor RraA/RraA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR010203","properties":[{"key":"EntryName","value":"RraA"}]},{"database":"InterPro","id":"IPR005493","properties":[{"key":"EntryName","value":"RraA/RraA-like"}]},{"database":"InterPro","id":"IPR036704","properties":[{"key":"EntryName","value":"RraA/RraA-like_sf"}]},{"database":"NCBIfam","id":"TIGR01935","properties":[{"key":"EntryName","value":"NOT-MenG"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009134","properties":[{"key":"EntryName","value":"PRK12487.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF006875","properties":[{"key":"EntryName","value":"rraA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33254","properties":[{"key":"EntryName","value":"4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33254:SF4","properties":[{"key":"EntryName","value":"4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03737","properties":[{"key":"EntryName","value":"RraA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF89562","properties":[{"key":"EntryName","value":"RraA-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MWQKFSQGGYMKDITPDICDQHEDKVTLLNLPLQNFGQKAAFFGEIVTVRCYHDNSKVRDVLSENGKGKVLVVDGHGSCQKALLGDQLAILAIENDWEGVIVFGAVRDVAQMSQMELGIKALGTSPFKTEKRGAGEVNVTLTMHNQMVQPKDYIYADWNGILISKELLQF","length":170,"molWeight":18948,"crc64":"955327A0CA5B5390","md5":"52A51D050FCFD3399858ECF28E2FE573"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":2,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Binding site":3},"uniParcId":"UPI00001D4D7E"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"C7A8W7","uniProtkbId":"C7A8W7_VIBVL","entryAudit":{"firstPublicDate":"2009-09-22","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2009-09-22","entryVersion":66,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACN39577.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00050038"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"value":"Peptidyl-tRNA hydrolase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"value":"Pth"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013260"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"value":"3.1.1.29"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACN39577.1"}],"value":"pth"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"value":"Catalyzes the release of premature peptidyl moieties from peptidyl-tRNA molecules trapped in stalled 50S ribosomal subunits, and thus maintains levels of free tRNAs and 50S ribosomes"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"value":"Hydrolyzes ribosome-free peptidyl-tRNAs (with 1 or more amino acids incorporated), which drop off the ribosome during protein synthesis, or as a result of ribosome stalling"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:54448"},{"database":"Rhea","id":"RHEA-COMP:10123"},{"database":"Rhea","id":"RHEA-COMP:13883"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:59874"},{"database":"ChEBI","id":"CHEBI:78442"},{"database":"ChEBI","id":"CHEBI:138191"}],"ecNumber":"3.1.1.29","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000673"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00038063"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004320"}],"value":"Belongs to the PTH family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Active site","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}]},{"type":"Binding site","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17843"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"ligand":{"name":"tRNA","id":"ChEBI:CHEBI:17843"}},{"type":"Binding site","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17843"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"ligand":{"name":"tRNA","id":"ChEBI:CHEBI:17843"}},{"type":"Binding site","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17843"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"ligand":{"name":"tRNA","id":"ChEBI:CHEBI:17843"}},{"type":"Binding site","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17843"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"ligand":{"name":"tRNA","id":"ChEBI:CHEBI:17843"}},{"type":"Site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Discriminates between blocked and unblocked aminoacyl-tRNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}]},{"type":"Site","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"Stabilizes the basic form of H active site to accept a proton","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022884"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022555"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00083"}],"id":"KW-0820","category":"Molecular function","name":"tRNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-4RBJPS0L4K1PD","citationType":"submission","authors":["Chen Y.-C.","Chung Y.-T."],"publicationDate":"SEP-2008","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACN39577.1"}]},{"referenceNumber":2,"citation":{"id":"21570909","citationType":"journal article","authors":["Chen Y.C.","Chung Y.T."],"citationCrossReferences":[{"database":"PubMed","id":"21570909"},{"database":"DOI","id":"10.1016/j.ijmm.2011.02.002"}],"title":"A conserved GTPase YchF of Vibrio vulnificus is involved in macrophage cytotoxicity, iron acquisition, and mouse virulence.","publicationDate":"2011","journal":"Int. J. Med. Microbiol.","firstPage":"469","lastPage":"474","volume":"301"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ACN39577.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"FJ237365","properties":[{"key":"ProteinId","value":"ACN39577.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"C7A8W7","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"vvl:VV93_v1c08570","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|672.132.peg.881","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"OMA","id":"AACWLFE","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0004045","properties":[{"key":"GoTerm","value":"F:peptidyl-tRNA hydrolase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000049","properties":[{"key":"GoTerm","value":"F:tRNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006515","properties":[{"key":"GoTerm","value":"P:protein quality control for misfolded or incompletely synthesized proteins"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0072344","properties":[{"key":"GoTerm","value":"P:rescue of stalled cytosolic ribosome"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00462","properties":[{"key":"EntryName","value":"PTH"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.1470:FF:000001","properties":[{"key":"EntryName","value":"Peptidyl-tRNA hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1470","properties":[{"key":"EntryName","value":"Peptidyl-tRNA hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00083","properties":[{"key":"EntryName","value":"Pept_tRNA_hydro_bact"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001328","properties":[{"key":"EntryName","value":"Pept_tRNA_hydro"}]},{"database":"InterPro","id":"IPR018171","properties":[{"key":"EntryName","value":"Pept_tRNA_hydro_CS"}]},{"database":"InterPro","id":"IPR036416","properties":[{"key":"EntryName","value":"Pept_tRNA_hydro_sf"}]},{"database":"NCBIfam","id":"TIGR00447","properties":[{"key":"EntryName","value":"pth"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR17224","properties":[{"key":"EntryName","value":"PEPTIDYL-TRNA HYDROLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR17224:SF1","properties":[{"key":"EntryName","value":"PEPTIDYL-TRNA HYDROLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01195","properties":[{"key":"EntryName","value":"Pept_tRNA_hydro"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53178","properties":[{"key":"EntryName","value":"Peptidyl-tRNA hydrolase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01195","properties":[{"key":"EntryName","value":"PEPT_TRNA_HYDROL_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01196","properties":[{"key":"EntryName","value":"PEPT_TRNA_HYDROL_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLSYRAFEDSKEILTLSQQIKLLVGLANPGPEYAKTRHNAGAWVVEELARVHNITLKNEAKFYGLTGRIVMNGQELRLLIPTTFMNLSGKAIAALAKFYQIQPEEIMVAHDELDLPPGIAKFKKGGGHGGHNGLRDTISKLGNNKEFYRLRIGIGHPGHKDKVAGFVLGKAPASEQSLLDATVDESVRCLDILIKDGLSKAQNRLHTFKAE","length":211,"molWeight":23210,"crc64":"0A7C67B22E7BBE08","md5":"34B6A69F18418AA9A180A834AE7D4045"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Active site":1,"Binding site":4,"Site":2},"uniParcId":"UPI00001D4E58"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"E5F0S5","uniProtkbId":"E5F0S5_VIBVL","entryAudit":{"firstPublicDate":"2011-02-08","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2011-02-08","entryVersion":36,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64236.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00019595"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"dTDP-4-dehydrorhamnose 3,5-epimerase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012098"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"5.1.3.13"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Thymidine diphospho-4-keto-rhamnose 3,5-epimerase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64236.1"}],"value":"rmlC"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001997"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16969"},{"database":"ChEBI","id":"CHEBI:57649"},{"database":"ChEBI","id":"CHEBI:62830"}],"ecNumber":"5.1.3.13","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001298"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Active site","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600888-1"}]},{"type":"Active site","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600888-1"}]},{"type":"Site","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600888-3"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"17923517","citationType":"journal article","authors":["Nakhamchik A.","Wilde C.","Rowe-Magnus D.A."],"citationCrossReferences":[{"database":"PubMed","id":"17923517"},{"database":"DOI","id":"10.1128/IAI.00932-07"}],"title":"Identification of a Wzy polymerase required for group IV capsular polysaccharide and lipopolysaccharide biosynthesis in Vibrio vulnificus.","publicationDate":"2007","journal":"Infect. Immun.","firstPage":"5550","lastPage":"5558","volume":"75"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64236.1"}],"value":"ATCC 27562","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64236.1"}]},{"referenceNumber":2,"citation":{"id":"20921143","citationType":"journal article","authors":["Nakhamchik A.","Wilde C.","Chong H.","Rowe-Magnus D.A."],"citationCrossReferences":[{"database":"PubMed","id":"20921143"},{"database":"DOI","id":"10.1128/IAI.00653-10"}],"title":"Evidence for the horizontal transfer of an unusual capsular polysaccharide biosynthesis locus in marine bacteria.","publicationDate":"2010","journal":"Infect. Immun.","firstPage":"5214","lastPage":"5222","volume":"78"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64236.1"}],"value":"ATCC 27562","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64236.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"HM099886","properties":[{"key":"ProteinId","value":"ADO64236.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_017420706.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP044069.1"}]},{"database":"AlphaFoldDB","id":"E5F0S5","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93895083","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00124","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0008830","properties":[{"key":"GoTerm","value":"F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019305","properties":[{"key":"GoTerm","value":"P:dTDP-rhamnose biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000271","properties":[{"key":"GoTerm","value":"P:polysaccharide biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd00438","properties":[{"key":"EntryName","value":"cupin_RmlC"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.10","properties":[{"key":"EntryName","value":"Jelly Rolls"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR000888","properties":[{"key":"EntryName","value":"RmlC-like"}]},{"database":"InterPro","id":"IPR014710","properties":[{"key":"EntryName","value":"RmlC-like_jellyroll"}]},{"database":"InterPro","id":"IPR011051","properties":[{"key":"EntryName","value":"RmlC_Cupin_sf"}]},{"database":"NCBIfam","id":"TIGR01221","properties":[{"key":"EntryName","value":"rmlC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21047","properties":[{"key":"EntryName","value":"DTDP-6-DEOXY-D-GLUCOSE-3,5 EPIMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21047:SF2","properties":[{"key":"EntryName","value":"THYMIDINE DIPHOSPHO-4-KETO-RHAMNOSE 3,5-EPIMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00908","properties":[{"key":"EntryName","value":"dTDP_sugar_isom"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51182","properties":[{"key":"EntryName","value":"RmlC-like cupins"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKVIDTNIPDVKIIEPTVFGDERGFFMETWNQKRFEELVTGKPTQFVQDNHSKSKKGILRGLHYQTENTQGKLVRVVSGEVFDVAVDIRKDSPTFGQWVGEYLSAENKRQLWIPEGFAHGFYVTSNEAEFVYKCTDYYNPDSEVSIKWDDETLKIQWPLANMPILSNKDLAAQNFLESELL","length":181,"molWeight":20903,"crc64":"711D8A4382544D6F","md5":"AED08A77BBDD02657B3BD04AA2787532"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Active site":2,"Site":1},"uniParcId":"UPI0001F24D8B"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"E5F0S6","uniProtkbId":"E5F0S6_VIBVL","entryAudit":{"firstPublicDate":"2011-02-08","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2011-02-08","entryVersion":62,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64237.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00015188"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"value":"UDP-N-acetylenolpyruvoylglucosamine reductase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012518"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"value":"1.3.1.98"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031026"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"value":"UDP-N-acetylmuramate dehydrogenase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"value":"murB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003921"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"value":"Cell wall formation"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"UDP-N-acetyl-alpha-D-muramate + NADP(+) = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:12248"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:68483"},{"database":"ChEBI","id":"CHEBI:70757"}],"ecNumber":"1.3.1.98","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048914"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"FAD","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001974"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:57692"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004752"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"value":"Cell wall biogenesis; peptidoglycan biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010485"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"value":"Belongs to the MurB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"FAD-binding PCMH-type","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51387"}]},{"type":"Active site","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}]},{"type":"Active site","location":{"start":{"value":232,"modifier":"EXACT"},"end":{"value":232,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}]},{"type":"Active site","location":{"start":{"value":327,"modifier":"EXACT"},"end":{"value":327,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023306"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022618"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0132","category":"Biological process","name":"Cell division"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022960"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0133","category":"Biological process","name":"Cell shape"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023316"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022827"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0274","category":"Ligand","name":"FAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022630"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022984"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00037"}],"id":"KW-0573","category":"Biological process","name":"Peptidoglycan synthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"17923517","citationType":"journal article","authors":["Nakhamchik A.","Wilde C.","Rowe-Magnus D.A."],"citationCrossReferences":[{"database":"PubMed","id":"17923517"},{"database":"DOI","id":"10.1128/IAI.00932-07"}],"title":"Identification of a Wzy polymerase required for group IV capsular polysaccharide and lipopolysaccharide biosynthesis in Vibrio vulnificus.","publicationDate":"2007","journal":"Infect. Immun.","firstPage":"5550","lastPage":"5558","volume":"75"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64237.1"}],"value":"ATCC 27562","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64237.1"}]},{"referenceNumber":2,"citation":{"id":"20921143","citationType":"journal article","authors":["Nakhamchik A.","Wilde C.","Chong H.","Rowe-Magnus D.A."],"citationCrossReferences":[{"database":"PubMed","id":"20921143"},{"database":"DOI","id":"10.1128/IAI.00653-10"}],"title":"Evidence for the horizontal transfer of an unusual capsular polysaccharide biosynthesis locus in marine bacteria.","publicationDate":"2010","journal":"Infect. Immun.","firstPage":"5214","lastPage":"5222","volume":"78"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64237.1"}],"value":"ATCC 27562","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64237.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"HM099886","properties":[{"key":"ProteinId","value":"ADO64237.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_017420705.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP044069.1"}]},{"database":"AlphaFoldDB","id":"E5F0S6","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93895082","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00219","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0071949","properties":[{"key":"GoTerm","value":"F:FAD binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008762","properties":[{"key":"GoTerm","value":"F:UDP-N-acetylmuramate dehydrogenase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0071555","properties":[{"key":"GoTerm","value":"P:cell wall organization"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009252","properties":[{"key":"GoTerm","value":"P:peptidoglycan biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"3.30.465.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.78.10","properties":[{"key":"EntryName","value":"UDP-N-acetylenolpyruvoylglucosamine reductase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.43.10","properties":[{"key":"EntryName","value":"Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00037","properties":[{"key":"EntryName","value":"MurB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR016166","properties":[{"key":"EntryName","value":"FAD-bd_PCMH"}]},{"database":"InterPro","id":"IPR036318","properties":[{"key":"EntryName","value":"FAD-bd_PCMH-like_sf"}]},{"database":"InterPro","id":"IPR016167","properties":[{"key":"EntryName","value":"FAD-bd_PCMH_sub1"}]},{"database":"InterPro","id":"IPR016169","properties":[{"key":"EntryName","value":"FAD-bd_PCMH_sub2"}]},{"database":"InterPro","id":"IPR003170","properties":[{"key":"EntryName","value":"MurB"}]},{"database":"InterPro","id":"IPR011601","properties":[{"key":"EntryName","value":"MurB_C"}]},{"database":"InterPro","id":"IPR036635","properties":[{"key":"EntryName","value":"MurB_C_sf"}]},{"database":"InterPro","id":"IPR006094","properties":[{"key":"EntryName","value":"Oxid_FAD_bind_N"}]},{"database":"NCBIfam","id":"NF000755","properties":[{"key":"EntryName","value":"murB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00179","properties":[{"key":"EntryName","value":"murB"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21071","properties":[{"key":"EntryName","value":"UDP-N-ACETYLENOLPYRUVOYLGLUCOSAMINE REDUCTASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21071:SF4","properties":[{"key":"EntryName","value":"UDP-N-ACETYLENOLPYRUVOYLGLUCOSAMINE REDUCTASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01565","properties":[{"key":"EntryName","value":"FAD_binding_4"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02873","properties":[{"key":"EntryName","value":"MurB_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56176","properties":[{"key":"EntryName","value":"FAD-binding/transporter-associated domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56194","properties":[{"key":"EntryName","value":"Uridine diphospho-N-Acetylenolpyruvylglucosamine reductase, MurB, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51387","properties":[{"key":"EntryName","value":"FAD_PCMH"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIEKFNVQMRDFNTLKLSSVAERFYEIFDENELSDVFGNKINAEQDFRVLGAGSNVWLSSDSIPVVIQLNVEGISIERKEETTSVAAKAGENWSLFVEFLVSEGIGGLEFLIDIPGTVGAAPIQNIGAYGVEVGEYITSVKVFDTNNMEYKYIPNENCMFGYRDSIFKKDKTLIVTEVFFEFRNDYIPNIKNSEIVQEISGSDVTIDLILNAVRKIRKNKIPDPKILPNSGSFFKNPILKEEKLKQIKSEYDDIVFYKLNSGYKVSAAWIIEKAGWKGKIINGVGMSSMHSLILTNSSNNNLVKIDEYVKLLKQDVLRKFDVELEVEPACW","length":331,"molWeight":37480,"crc64":"9CCF88431EC7F243","md5":"286E6ABB369B672EE0745228D1AC5C8D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Active site":3},"uniParcId":"UPI0001F24DD6"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"E5F0S7","uniProtkbId":"E5F0S7_VIBVL","entryAudit":{"firstPublicDate":"2011-02-08","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-02-08","entryVersion":58,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64238.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"UDP-N-acetylglucosamine 1-carboxyvinyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"2.5.1.7"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"Enoylpyruvate transferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"UDP-N-acetylglucosamine enolpyruvyl transferase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"EPT"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64238.1"}],"value":"murA2"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"murA"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18681"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57705"},{"database":"ChEBI","id":"CHEBI:58702"},{"database":"ChEBI","id":"CHEBI:68483"}],"ecNumber":"2.5.1.7","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047527"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004752"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"Cell wall biogenesis; peptidoglycan biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00038367"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"Belongs to the EPSP synthase family. MurA subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":376,"modifier":"EXACT"}},"description":"Enolpyruvate transferase","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00275"}]},{"type":"Active site","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}]},{"type":"Binding site","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57705"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-glucosamine","id":"ChEBI:CHEBI:57705"}},{"type":"Binding site","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":97,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57705"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-glucosamine","id":"ChEBI:CHEBI:57705"}},{"type":"Binding site","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":275,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57705"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-glucosamine","id":"ChEBI:CHEBI:57705"}},{"type":"Binding site","location":{"start":{"value":297,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57705"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-glucosamine","id":"ChEBI:CHEBI:57705"}},{"type":"Modified residue","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"2-(S-cysteinyl)pyruvic acid O-phosphothioketal","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023306"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022618"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"id":"KW-0132","category":"Biological process","name":"Cell division"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022960"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"id":"KW-0133","category":"Biological process","name":"Cell shape"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023316"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022984"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"id":"KW-0573","category":"Biological process","name":"Peptidoglycan synthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023317"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"id":"KW-0670","category":"Ligand","name":"Pyruvate"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00111"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"17923517","citationType":"journal article","authors":["Nakhamchik A.","Wilde C.","Rowe-Magnus D.A."],"citationCrossReferences":[{"database":"PubMed","id":"17923517"},{"database":"DOI","id":"10.1128/IAI.00932-07"}],"title":"Identification of a Wzy polymerase required for group IV capsular polysaccharide and lipopolysaccharide biosynthesis in Vibrio vulnificus.","publicationDate":"2007","journal":"Infect. Immun.","firstPage":"5550","lastPage":"5558","volume":"75"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64238.1"}],"value":"ATCC 27562","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64238.1"}]},{"referenceNumber":2,"citation":{"id":"20921143","citationType":"journal article","authors":["Nakhamchik A.","Wilde C.","Chong H.","Rowe-Magnus D.A."],"citationCrossReferences":[{"database":"PubMed","id":"20921143"},{"database":"DOI","id":"10.1128/IAI.00653-10"}],"title":"Evidence for the horizontal transfer of an unusual capsular polysaccharide biosynthesis locus in marine bacteria.","publicationDate":"2010","journal":"Infect. Immun.","firstPage":"5214","lastPage":"5222","volume":"78"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64238.1"}],"value":"ATCC 27562","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64238.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"HM099886","properties":[{"key":"ProteinId","value":"ADO64238.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"E5F0S7","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00219","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008760","properties":[{"key":"GoTerm","value":"F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009252","properties":[{"key":"GoTerm","value":"P:peptidoglycan biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019277","properties":[{"key":"GoTerm","value":"P:UDP-N-acetylgalactosamine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd01555","properties":[{"key":"EntryName","value":"UdpNAET"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.65.10.10:FF:000001","properties":[{"key":"EntryName","value":"UDP-N-acetylglucosamine 1-carboxyvinyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.65.10.10","properties":[{"key":"EntryName","value":"Enolpyruvate transferase domain"},{"key":"MatchStatus","value":"2"}]},{"database":"HAMAP","id":"MF_00111","properties":[{"key":"EntryName","value":"MurA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001986","properties":[{"key":"EntryName","value":"Enolpyruvate_Tfrase_dom"}]},{"database":"InterPro","id":"IPR036968","properties":[{"key":"EntryName","value":"Enolpyruvate_Tfrase_sf"}]},{"database":"InterPro","id":"IPR013792","properties":[{"key":"EntryName","value":"RNA3'P_cycl/enolpyr_Trfase_a/b"}]},{"database":"InterPro","id":"IPR005750","properties":[{"key":"EntryName","value":"UDP_GlcNAc_COvinyl_MurA"}]},{"database":"NCBIfam","id":"TIGR01072","properties":[{"key":"EntryName","value":"murA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF006873","properties":[{"key":"EntryName","value":"PRK09369.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43783","properties":[{"key":"EntryName","value":"UDP-N-ACETYLGLUCOSAMINE 1-CARBOXYVINYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43783:SF1","properties":[{"key":"EntryName","value":"UDP-N-ACETYLGLUCOSAMINE 1-CARBOXYVINYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00275","properties":[{"key":"EntryName","value":"EPSP_synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55205","properties":[{"key":"EntryName","value":"EPT/RTPC-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MCAALLVEGKLTLSNVPRLKDVYTLVDILGSMGAKSEFNNNCLELDCSDLTSTFASYELVKTMRASILVLGPLLARFGKAKVSLPGGCAIGSRPVDLHLKALESLGAEIHIEDGYVIAEGKLKGSEVYFDIPTVGGTENTILAATLAKGTTIINNAAMEPEIVDLCDCLRGMGAIISGDGTSCITIEGVKKLNNAKHSVIADRIEAGTFIIASAVTNSNLTLTNIRKKDLTSILSKLTQAGISLVETESGLTVYRDANLKAVDIQTQPHPGFPTDMQAQFTVLNCIADGVGTVEETIFENRFMHVAELNRMNANISIHGNYAKIEGSRKLKAATVMATDLRASASLVIAGLVAEGTTIVDRIYHLDRGYENIENKLAAVGASVKRVK","length":387,"molWeight":41229,"crc64":"6829A06B72DAA597","md5":"AFE9A03C1664F50E7FBE6054F432209B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Active site":1,"Binding site":4,"Modified residue":1},"uniParcId":"UPI0001F24DD7"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"E5F0T5","uniProtkbId":"E5F0T5_VIBVL","entryAudit":{"firstPublicDate":"2011-02-08","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2011-02-08","entryVersion":40,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64246.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"2.7.8.33"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Undecaprenyl-phosphate GlcNAc-1-phosphate transferase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64246.1"}],"value":"wecA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Catalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP-C55)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl diphosphate + UMP","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:28090"},{"database":"ChEBI","id":"CHEBI:57705"},{"database":"ChEBI","id":"CHEBI:57865"},{"database":"ChEBI","id":"CHEBI:60392"},{"database":"ChEBI","id":"CHEBI:62959"}],"ecNumber":"2.7.8.33","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600715-1"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004651"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004651"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Belongs to the glycosyltransferase 4 family. WecA subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":156,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":290,"modifier":"EXACT"},"end":{"value":307,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":319,"modifier":"EXACT"},"end":{"value":337,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Binding site","location":{"start":{"value":148,"modifier":"EXACT"},"end":{"value":148,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600715-1"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600715-1"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022519"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022475"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022676"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022985"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0448","category":"Biological process","name":"Lipopolysaccharide biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023211"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0464","category":"Ligand","name":"Manganese"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600715-1"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"17923517","citationType":"journal article","authors":["Nakhamchik A.","Wilde C.","Rowe-Magnus D.A."],"citationCrossReferences":[{"database":"PubMed","id":"17923517"},{"database":"DOI","id":"10.1128/IAI.00932-07"}],"title":"Identification of a Wzy polymerase required for group IV capsular polysaccharide and lipopolysaccharide biosynthesis in Vibrio vulnificus.","publicationDate":"2007","journal":"Infect. Immun.","firstPage":"5550","lastPage":"5558","volume":"75"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64246.1"}],"value":"ATCC 27562","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64246.1"}]},{"referenceNumber":2,"citation":{"id":"20921143","citationType":"journal article","authors":["Nakhamchik A.","Wilde C.","Chong H.","Rowe-Magnus D.A."],"citationCrossReferences":[{"database":"PubMed","id":"20921143"},{"database":"DOI","id":"10.1128/IAI.00653-10"}],"title":"Evidence for the horizontal transfer of an unusual capsular polysaccharide biosynthesis locus in marine bacteria.","publicationDate":"2010","journal":"Infect. Immun.","firstPage":"5214","lastPage":"5222","volume":"78"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64246.1"}],"value":"ATCC 27562","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADO64246.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"HM099886","properties":[{"key":"ProteinId","value":"ADO64246.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_017420697.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP044069.1"}]},{"database":"AlphaFoldDB","id":"E5F0T5","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93895073","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00281","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009276","properties":[{"key":"GoTerm","value":"C:Gram-negative-bacterium-type cell wall"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0030145","properties":[{"key":"GoTerm","value":"F:manganese ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0036380","properties":[{"key":"GoTerm","value":"F:UDP-N-acetylglucosamine-undecaprenyl-phosphate N-acetylglucosaminephosphotransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0044038","properties":[{"key":"GoTerm","value":"P:cell wall macromolecule biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0071555","properties":[{"key":"GoTerm","value":"P:cell wall organization"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009243","properties":[{"key":"GoTerm","value":"P:O antigen biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd06853","properties":[{"key":"EntryName","value":"GT_WecA_like"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_02030","properties":[{"key":"EntryName","value":"WecA_Gammaproteo"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR012750","properties":[{"key":"EntryName","value":"ECA_WecA-rel"}]},{"database":"InterPro","id":"IPR000715","properties":[{"key":"EntryName","value":"Glycosyl_transferase_4"}]},{"database":"NCBIfam","id":"TIGR02380","properties":[{"key":"EntryName","value":"ECA_wecA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR22926","properties":[{"key":"EntryName","value":"PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR22926:SF3","properties":[{"key":"EntryName","value":"UNDECAPRENYL-PHOSPHATE ALPHA-N-ACETYLGLUCOSAMINYL 1-PHOSPHATE TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00953","properties":[{"key":"EntryName","value":"Glycos_transf_4"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFLELSFIFFSSFATLFLMRKVAKRIGLVDKPNARKLHTGSVPLVGGISICLVLAQYLVFRPETINHSWLYLASIMLLTFVGALDDKFDLSFKIRMGIQAALSLVMMHAAGIELHSLGNLFGVGEIYLGWMGSIITILAVIGAINAFNMVDGIDGLLGGLSIVTFGALAFLLNVDSQHGLAYLCVVIIVAMLPYILMNLGILGRERKVFMGDAGSMMIGFTVIWLLLGVSQVESKPLMRPVTALWLIAVPLMDMAAIMIRRVRRGDSPFKPDREHLHHIFQRLGLTSRQTLVVICGFATLFAAFGIYGEATKISEAFMFYSFISCFLIYSTLLNYVWRITSFARRILGKGQTEGAEQKA","length":359,"molWeight":39693,"crc64":"8DB2E68CB7CB3BB8","md5":"4E14AB2592F75AE59F5883212EA99BBE"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":11,"Binding site":2},"uniParcId":"UPI00015E4C73"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"F1ARZ4","uniProtkbId":"F1ARZ4_VIBVL","entryAudit":{"firstPublicDate":"2011-05-03","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2011-05-03","entryVersion":55,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33016.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011952"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Glucose-6-phosphate isomerase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011952"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"5.3.1.9"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"pgi"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11816"},{"database":"ChEBI","id":"CHEBI:57634"},{"database":"ChEBI","id":"CHEBI:58225"}],"ecNumber":"5.3.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11816"},{"database":"ChEBI","id":"CHEBI:57634"},{"database":"ChEBI","id":"CHEBI:58225"}],"ecNumber":"5.3.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Belongs to the GPI family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Active site","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33016.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33016.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022432"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0312","category":"Biological process","name":"Gluconeogenesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023152"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0324","category":"Biological process","name":"Glycolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"22227412","citationType":"journal article","authors":["Broza Y.Y.","Raz N.","Lerner L.","Danin-Poleg Y.","Kashi Y."],"citationCrossReferences":[{"database":"PubMed","id":"22227412"},{"database":"DOI","id":"10.1016/j.ijfoodmicro.2011.12.011"}],"title":"Genetic diversity of the human pathogen Vibrio vulnificus: A new phylogroup.","publicationDate":"2012","journal":"Int. J. Food Microbiol.","firstPage":"436","lastPage":"443","volume":"153"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33036.1"}],"value":"145/96","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33043.1"}],"value":"2/04","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33039.1"}],"value":"2321","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33041.1"}],"value":"3/06","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33042.1"}],"value":"4/05","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33037.1"}],"value":"58/97","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33038.1"}],"value":"7/03","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33044.1"}],"value":"7/04","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33040.1"}],"value":"8/03","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33016.1"}],"value":"VVyb1","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33020.1"}],"value":"VVyb110","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33021.1"}],"value":"VVyb158","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33022.1"}],"value":"VVyb160","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33023.1"}],"value":"VVyb161","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33024.1"}],"value":"VVyb171","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33025.1"}],"value":"VVyb180","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33027.1"}],"value":"VVyb183","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33028.1"}],"value":"VVyb184","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33029.1"}],"value":"VVyb185","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33030.1"}],"value":"VVyb188","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33031.1"}],"value":"VVyb199","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33032.1"}],"value":"VVyb209","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33033.1"}],"value":"VVyb210","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33034.1"}],"value":"VVyb217","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33017.1"}],"value":"VVyb5","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33019.1"}],"value":"VVyb95","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33016.1"}]},{"referenceNumber":2,"citation":{"id":"CI-6CQEQ2HSA7U2J","citationType":"submission","authors":["Guerrero A.","Gomez-Gil B.","Wong-Ghang I.","Lizarraga-Partida M.L."],"title":"Genetic characterization of Vibrio vulnificus strains isolated from oyster samples in Mexico.","publicationDate":"JUL-2014","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39676.1"}],"value":"CICESE-300","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39677.1"}],"value":"CICESE-302","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39678.1"}],"value":"CICESE-306","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39679.1"}],"value":"CICESE-314","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39680.1"}],"value":"CICESE-316","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39681.1"}],"value":"CICESE-319","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39682.1"}],"value":"CICESE-320","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39683.1"}],"value":"CICESE-321","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39684.1"}],"value":"CICESE-325","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39685.1"}],"value":"CICESE-332","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39686.1"}],"value":"CICESE-335","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39687.1"}],"value":"CICESE-342","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39688.1"}],"value":"CICESE-345","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39689.1"}],"value":"CICESE-346","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39690.1"}],"value":"CICESE-357","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39691.1"}],"value":"CICESE-363","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39692.1"}],"value":"CICESE-368","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39693.1"}],"value":"CICESE-369","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AIS39676.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"GU999590","properties":[{"key":"ProteinId","value":"ADX33016.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999591","properties":[{"key":"ProteinId","value":"ADX33017.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999593","properties":[{"key":"ProteinId","value":"ADX33019.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999594","properties":[{"key":"ProteinId","value":"ADX33020.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999595","properties":[{"key":"ProteinId","value":"ADX33021.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999596","properties":[{"key":"ProteinId","value":"ADX33022.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999597","properties":[{"key":"ProteinId","value":"ADX33023.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999598","properties":[{"key":"ProteinId","value":"ADX33024.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999599","properties":[{"key":"ProteinId","value":"ADX33025.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999601","properties":[{"key":"ProteinId","value":"ADX33027.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999602","properties":[{"key":"ProteinId","value":"ADX33028.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999603","properties":[{"key":"ProteinId","value":"ADX33029.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999604","properties":[{"key":"ProteinId","value":"ADX33030.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999605","properties":[{"key":"ProteinId","value":"ADX33031.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999606","properties":[{"key":"ProteinId","value":"ADX33032.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999607","properties":[{"key":"ProteinId","value":"ADX33033.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999608","properties":[{"key":"ProteinId","value":"ADX33034.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999610","properties":[{"key":"ProteinId","value":"ADX33036.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999611","properties":[{"key":"ProteinId","value":"ADX33037.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999612","properties":[{"key":"ProteinId","value":"ADX33038.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999613","properties":[{"key":"ProteinId","value":"ADX33039.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999614","properties":[{"key":"ProteinId","value":"ADX33040.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999615","properties":[{"key":"ProteinId","value":"ADX33041.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999616","properties":[{"key":"ProteinId","value":"ADX33042.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999617","properties":[{"key":"ProteinId","value":"ADX33043.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999618","properties":[{"key":"ProteinId","value":"ADX33044.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999619","properties":[{"key":"ProteinId","value":"ADX33045.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107762","properties":[{"key":"ProteinId","value":"AIS39676.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107763","properties":[{"key":"ProteinId","value":"AIS39677.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107764","properties":[{"key":"ProteinId","value":"AIS39678.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107765","properties":[{"key":"ProteinId","value":"AIS39679.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107766","properties":[{"key":"ProteinId","value":"AIS39680.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107767","properties":[{"key":"ProteinId","value":"AIS39681.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107768","properties":[{"key":"ProteinId","value":"AIS39682.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107769","properties":[{"key":"ProteinId","value":"AIS39683.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107770","properties":[{"key":"ProteinId","value":"AIS39684.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107771","properties":[{"key":"ProteinId","value":"AIS39685.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107772","properties":[{"key":"ProteinId","value":"AIS39686.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107773","properties":[{"key":"ProteinId","value":"AIS39687.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107774","properties":[{"key":"ProteinId","value":"AIS39688.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107775","properties":[{"key":"ProteinId","value":"AIS39689.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107776","properties":[{"key":"ProteinId","value":"AIS39690.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107777","properties":[{"key":"ProteinId","value":"AIS39691.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107778","properties":[{"key":"ProteinId","value":"AIS39692.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KM107779","properties":[{"key":"ProteinId","value":"AIS39693.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"F1ARZ4","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00109","properties":[{"key":"RectionId","value":"UER00181"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0097367","properties":[{"key":"GoTerm","value":"F:carbohydrate derivative binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004347","properties":[{"key":"GoTerm","value":"F:glucose-6-phosphate isomerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0048029","properties":[{"key":"GoTerm","value":"F:monosaccharide binding"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006094","properties":[{"key":"GoTerm","value":"P:gluconeogenesis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051156","properties":[{"key":"GoTerm","value":"P:glucose 6-phosphate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd05016","properties":[{"key":"EntryName","value":"SIS_PGI_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.10490","properties":[{"key":"EntryName","value":"Glucose-6-phosphate isomerase like protein, domain 1"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR001672","properties":[{"key":"EntryName","value":"G6P_Isomerase"}]},{"database":"InterPro","id":"IPR018189","properties":[{"key":"EntryName","value":"Phosphoglucose_isomerase_CS"}]},{"database":"InterPro","id":"IPR046348","properties":[{"key":"EntryName","value":"SIS_dom_sf"}]},{"database":"InterPro","id":"IPR035482","properties":[{"key":"EntryName","value":"SIS_PGI_2"}]},{"database":"PANTHER","id":"PTHR11469","properties":[{"key":"EntryName","value":"GLUCOSE-6-PHOSPHATE ISOMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11469:SF1","properties":[{"key":"EntryName","value":"GLUCOSE-6-PHOSPHATE ISOMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00342","properties":[{"key":"EntryName","value":"PGI"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00662","properties":[{"key":"EntryName","value":"G6PISOMERASE"}]},{"database":"SUPFAM","id":"SSF53697","properties":[{"key":"EntryName","value":"SIS domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00765","properties":[{"key":"EntryName","value":"P_GLUCOSE_ISOMERASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51463","properties":[{"key":"EntryName","value":"P_GLUCOSE_ISOMERASE_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"NAHTARDWFLATAGDQAHVAKHFAALSTNAPAVSEFGIDTDNMFEFWDWVGGRYSLWSAIGLSIALAVGYDNFVELLEGAHEMDNHFVSTELESNIPVILALIGIWYNNFHGAESEAILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNPVTYQTGPIIW","length":160,"molWeight":17953,"crc64":"5EA96283FA0DD505","md5":"984F141C10214FDD974427A53A274927"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI0001FFCEC4"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"F1ARZ6","uniProtkbId":"F1ARZ6_VIBVL","entryAudit":{"firstPublicDate":"2011-05-03","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2011-05-03","entryVersion":54,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33018.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011952"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Glucose-6-phosphate isomerase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011952"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"5.3.1.9"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"pgi"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11816"},{"database":"ChEBI","id":"CHEBI:57634"},{"database":"ChEBI","id":"CHEBI:58225"}],"ecNumber":"5.3.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11816"},{"database":"ChEBI","id":"CHEBI:57634"},{"database":"ChEBI","id":"CHEBI:58225"}],"ecNumber":"5.3.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Belongs to the GPI family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Active site","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33018.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33018.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022432"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0312","category":"Biological process","name":"Gluconeogenesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023152"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0324","category":"Biological process","name":"Glycolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"22227412","citationType":"journal article","authors":["Broza Y.Y.","Raz N.","Lerner L.","Danin-Poleg Y.","Kashi Y."],"citationCrossReferences":[{"database":"PubMed","id":"22227412"},{"database":"DOI","id":"10.1016/j.ijfoodmicro.2011.12.011"}],"title":"Genetic diversity of the human pathogen Vibrio vulnificus: A new phylogroup.","publicationDate":"2012","journal":"Int. J. Food Microbiol.","firstPage":"436","lastPage":"443","volume":"153"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33035.1"}],"value":"03/04:95.8.161","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33018.1"}],"value":"VVyb82","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33018.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"GU999592","properties":[{"key":"ProteinId","value":"ADX33018.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"GU999609","properties":[{"key":"ProteinId","value":"ADX33035.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"F1ARZ6","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00109","properties":[{"key":"RectionId","value":"UER00181"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0097367","properties":[{"key":"GoTerm","value":"F:carbohydrate derivative binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004347","properties":[{"key":"GoTerm","value":"F:glucose-6-phosphate isomerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0048029","properties":[{"key":"GoTerm","value":"F:monosaccharide binding"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006094","properties":[{"key":"GoTerm","value":"P:gluconeogenesis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051156","properties":[{"key":"GoTerm","value":"P:glucose 6-phosphate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd05016","properties":[{"key":"EntryName","value":"SIS_PGI_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.10490","properties":[{"key":"EntryName","value":"Glucose-6-phosphate isomerase like protein, domain 1"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR001672","properties":[{"key":"EntryName","value":"G6P_Isomerase"}]},{"database":"InterPro","id":"IPR018189","properties":[{"key":"EntryName","value":"Phosphoglucose_isomerase_CS"}]},{"database":"InterPro","id":"IPR046348","properties":[{"key":"EntryName","value":"SIS_dom_sf"}]},{"database":"InterPro","id":"IPR035482","properties":[{"key":"EntryName","value":"SIS_PGI_2"}]},{"database":"PANTHER","id":"PTHR11469","properties":[{"key":"EntryName","value":"GLUCOSE-6-PHOSPHATE ISOMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11469:SF1","properties":[{"key":"EntryName","value":"GLUCOSE-6-PHOSPHATE ISOMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00342","properties":[{"key":"EntryName","value":"PGI"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00662","properties":[{"key":"EntryName","value":"G6PISOMERASE"}]},{"database":"SUPFAM","id":"SSF53697","properties":[{"key":"EntryName","value":"SIS domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00765","properties":[{"key":"EntryName","value":"P_GLUCOSE_ISOMERASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51463","properties":[{"key":"EntryName","value":"P_GLUCOSE_ISOMERASE_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"NAHTARDWFLATAGDQAHVAKHFAALSTNAPAVSEFGIDTDNMFEFWDWVGGRYSLWSAIGLSIALAVGYENFVELLEGAHEMDNHFVSTELESNIPVILALIGIWYNNFHGAESEAILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNPVTYQTGPIIW","length":160,"molWeight":17967,"crc64":"5EA87382EA1CD414","md5":"D035773A26DC00F63DCAF8FB5CED7B50"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI0001FFCEC6"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"F1AS04","uniProtkbId":"F1AS04_VIBVL","entryAudit":{"firstPublicDate":"2011-05-03","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2011-05-03","entryVersion":53,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33026.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011952"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Glucose-6-phosphate isomerase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011952"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"5.3.1.9"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"pgi"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11816"},{"database":"ChEBI","id":"CHEBI:57634"},{"database":"ChEBI","id":"CHEBI:58225"}],"ecNumber":"5.3.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11816"},{"database":"ChEBI","id":"CHEBI:57634"},{"database":"ChEBI","id":"CHEBI:58225"}],"ecNumber":"5.3.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Belongs to the GPI family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Active site","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33026.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33026.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022432"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0312","category":"Biological process","name":"Gluconeogenesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023152"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0324","category":"Biological process","name":"Glycolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"22227412","citationType":"journal article","authors":["Broza Y.Y.","Raz N.","Lerner L.","Danin-Poleg Y.","Kashi Y."],"citationCrossReferences":[{"database":"PubMed","id":"22227412"},{"database":"DOI","id":"10.1016/j.ijfoodmicro.2011.12.011"}],"title":"Genetic diversity of the human pathogen Vibrio vulnificus: A new phylogroup.","publicationDate":"2012","journal":"Int. J. Food Microbiol.","firstPage":"436","lastPage":"443","volume":"153"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33026.1"}],"value":"VVyb182","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADX33026.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"GU999600","properties":[{"key":"ProteinId","value":"ADX33026.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"F1AS04","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00109","properties":[{"key":"RectionId","value":"UER00181"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0097367","properties":[{"key":"GoTerm","value":"F:carbohydrate derivative binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004347","properties":[{"key":"GoTerm","value":"F:glucose-6-phosphate isomerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0048029","properties":[{"key":"GoTerm","value":"F:monosaccharide binding"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006094","properties":[{"key":"GoTerm","value":"P:gluconeogenesis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051156","properties":[{"key":"GoTerm","value":"P:glucose 6-phosphate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd05016","properties":[{"key":"EntryName","value":"SIS_PGI_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.10490","properties":[{"key":"EntryName","value":"Glucose-6-phosphate isomerase like protein, domain 1"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR001672","properties":[{"key":"EntryName","value":"G6P_Isomerase"}]},{"database":"InterPro","id":"IPR018189","properties":[{"key":"EntryName","value":"Phosphoglucose_isomerase_CS"}]},{"database":"InterPro","id":"IPR046348","properties":[{"key":"EntryName","value":"SIS_dom_sf"}]},{"database":"InterPro","id":"IPR035482","properties":[{"key":"EntryName","value":"SIS_PGI_2"}]},{"database":"PANTHER","id":"PTHR11469","properties":[{"key":"EntryName","value":"GLUCOSE-6-PHOSPHATE ISOMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11469:SF1","properties":[{"key":"EntryName","value":"GLUCOSE-6-PHOSPHATE ISOMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00342","properties":[{"key":"EntryName","value":"PGI"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00662","properties":[{"key":"EntryName","value":"G6PISOMERASE"}]},{"database":"SUPFAM","id":"SSF53697","properties":[{"key":"EntryName","value":"SIS domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00765","properties":[{"key":"EntryName","value":"P_GLUCOSE_ISOMERASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51463","properties":[{"key":"EntryName","value":"P_GLUCOSE_ISOMERASE_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"NAHTARDWFLATAGDQAHVAKHFAALSTNAPAVSEFGIDTDNMFEFWDWVGGRYSLWSAIGLSIALAVGYDNFIELLEGAHEMDNHFVSTELESNIPVILALIGIWYNNFHGAESEAILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNPVTYQTGPIIW","length":160,"molWeight":17967,"crc64":"A0588D9D150DC37F","md5":"B94B17538DD6FF94822602E756665CAC"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI0001FFCEC5"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q83WT9","uniProtkbId":"Q83WT9_VIBVL","entryAudit":{"firstPublicDate":"2003-06-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2003-06-01","entryVersion":98,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP33062.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"value":"Glucose-6-phosphate isomerase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"value":"GPI"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"value":"5.3.1.9"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"value":"Phosphoglucose isomerase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"value":"PGI"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"value":"Phosphohexose isomerase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"value":"PHI"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP33062.1"}],"value":"pgi"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"value":"Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11816"},{"database":"ChEBI","id":"CHEBI:57634"},{"database":"ChEBI","id":"CHEBI:58225"}],"ecNumber":"5.3.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11816"},{"database":"ChEBI","id":"CHEBI:57634"},{"database":"ChEBI","id":"CHEBI:58225"}],"ecNumber":"5.3.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"value":"Carbohydrate biosynthesis; gluconeogenesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004926"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006604"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000612"}],"value":"Belongs to the GPI family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Active site","location":{"start":{"value":356,"modifier":"EXACT"},"end":{"value":356,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]},{"type":"Active site","location":{"start":{"value":387,"modifier":"EXACT"},"end":{"value":387,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]},{"type":"Active site","location":{"start":{"value":515,"modifier":"EXACT"},"end":{"value":515,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00796"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022432"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"id":"KW-0312","category":"Biological process","name":"Gluconeogenesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023152"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"id":"KW-0324","category":"Biological process","name":"Glycolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00473"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"15262231","citationType":"journal article","authors":["Richards G.P.","Hammer C.H.","Garfield M.K.","Parveen S."],"citationCrossReferences":[{"database":"PubMed","id":"15262231"}],"title":"Characterization of a lysyl aminopeptidase activity associated with phosphoglucose isomerase of Vibrio vulnificus.","publicationDate":"2004","journal":"Biochim. Biophys. Acta","firstPage":"219","lastPage":"229","volume":"1700"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP33062.1"}],"value":"MLT364","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP33062.1"}]},{"referenceNumber":2,"citation":{"id":"15450853","citationType":"journal article","authors":["Richards G.P."],"citationCrossReferences":[{"database":"PubMed","id":"15450853"}],"title":"Structural and functional analyses of phosphoglucose isomerase from Vibrio vulnificus and its lysyl aminopeptidase activity.","publicationDate":"2004","journal":"Biochim. Biophys. Acta","firstPage":"89","lastPage":"102","volume":"1702"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP33062.1"}],"value":"MLT364","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAP33062.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY278944","properties":[{"key":"ProteinId","value":"AAP33062.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q83WT9","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AADEHFC","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00109","properties":[{"key":"RectionId","value":"UER00181"}]},{"database":"UniPathway","id":"UPA00138","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0097367","properties":[{"key":"GoTerm","value":"F:carbohydrate derivative binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004347","properties":[{"key":"GoTerm","value":"F:glucose-6-phosphate isomerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0048029","properties":[{"key":"GoTerm","value":"F:monosaccharide binding"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006094","properties":[{"key":"GoTerm","value":"P:gluconeogenesis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051156","properties":[{"key":"GoTerm","value":"P:glucose 6-phosphate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd05015","properties":[{"key":"EntryName","value":"SIS_PGI_1"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd05016","properties":[{"key":"EntryName","value":"SIS_PGI_2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.1390.10:FF:000001","properties":[{"key":"EntryName","value":"Glucose-6-phosphate isomerase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.10490:FF:000003","properties":[{"key":"EntryName","value":"Glucose-6-phosphate isomerase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.10490:FF:000004","properties":[{"key":"EntryName","value":"Glucose-6-phosphate isomerase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.1390.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.10490","properties":[{"key":"EntryName","value":"Glucose-6-phosphate isomerase like protein, domain 1"},{"key":"MatchStatus","value":"2"}]},{"database":"HAMAP","id":"MF_00473","properties":[{"key":"EntryName","value":"G6P_isomerase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001672","properties":[{"key":"EntryName","value":"G6P_Isomerase"}]},{"database":"InterPro","id":"IPR023096","properties":[{"key":"EntryName","value":"G6P_Isomerase_C"}]},{"database":"InterPro","id":"IPR018189","properties":[{"key":"EntryName","value":"Phosphoglucose_isomerase_CS"}]},{"database":"InterPro","id":"IPR046348","properties":[{"key":"EntryName","value":"SIS_dom_sf"}]},{"database":"InterPro","id":"IPR035476","properties":[{"key":"EntryName","value":"SIS_PGI_1"}]},{"database":"InterPro","id":"IPR035482","properties":[{"key":"EntryName","value":"SIS_PGI_2"}]},{"database":"NCBIfam","id":"NF001211","properties":[{"key":"EntryName","value":"pgi"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11469","properties":[{"key":"EntryName","value":"GLUCOSE-6-PHOSPHATE ISOMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11469:SF1","properties":[{"key":"EntryName","value":"GLUCOSE-6-PHOSPHATE ISOMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00342","properties":[{"key":"EntryName","value":"PGI"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00662","properties":[{"key":"EntryName","value":"G6PISOMERASE"}]},{"database":"SUPFAM","id":"SSF53697","properties":[{"key":"EntryName","value":"SIS domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00765","properties":[{"key":"EntryName","value":"P_GLUCOSE_ISOMERASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00174","properties":[{"key":"EntryName","value":"P_GLUCOSE_ISOMERASE_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51463","properties":[{"key":"EntryName","value":"P_GLUCOSE_ISOMERASE_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLKTINPTQTQAWNALTAHFESAQDMDLKDLFAQDAARFDKFSARFGSDILVDYSKNLINEETLKHLFALAKETELSAAIKAMFSGEAINQTEGRAVLHTALRNRSNQPVLVDGEDVMPAVNAVLEKMKSFTDRVIGGEWKGYTGKAITDIVNIGIGGSDLGPYMVTEALAPYKNHLNLHFVSNVDGTHIVETLKKVDPETTLFLIASKTFTTQETMTNAHTARDWFLATAGDQAHVAKHFAALSTNAPAVSEFGIDTDNMFEFWDWVGGRYSLWSAIGLSIALAVGYDNFVELLEGAHEMDNHFVSTELESNIPVILALIGIWYNNFHGAESEAILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNPVTYQTGPIIWGEPGTNGQHAFYQLIHQGTKLIPCDFIAPAVSHNPAGDHHQKLMSNFFAQTEALAFGKNEETVKAELVKVGKNAEEVAAIAPFKVFEGNRPTNSILVKQITPRTLGNLIAMYEHKIFVQGVIWNIFSFDQWGVELGKQLANQILPELADGSEISSHDSSTNGLINAFKAFKA","length":550,"molWeight":60772,"crc64":"B74DED03F2A26395","md5":"F5C7ED82D2603EF2639145B072BC0B36"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"PATHWAY":2,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Active site":3},"uniParcId":"UPI000019A45B"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q846Z2","uniProtkbId":"Q846Z2_VIBVL","entryAudit":{"firstPublicDate":"2003-06-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2003-06-01","entryVersion":85,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO65601.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013297"}],"value":"ADP-ribose pyrophosphatase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012453"}],"value":"3.6.1.13"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00030162"}],"value":"ADP-ribose diphosphatase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00033056"}],"value":"ADP-ribose phosphohydrolase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00030308"}],"value":"Adenosine diphosphoribose pyrophosphatase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO65601.1"}],"value":"mutT"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00025164"}],"value":"Acts on ADP-mannose and ADP-glucose as well as ADP-ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ADP-D-ribose + H2O = D-ribose 5-phosphate + AMP + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10412"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57967"},{"database":"ChEBI","id":"CHEBI:78346"},{"database":"ChEBI","id":"CHEBI:456215"}],"ecNumber":"3.6.1.13","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049546"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR604385-2"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007482"}],"value":"Belongs to the Nudix hydrolase family. NudF subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"Nudix hydrolase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51462"}]},{"type":"Motif","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"Nudix box","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR604385-3"}]},{"type":"Motif","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"Nudix box","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00794"}]},{"type":"Binding site","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR604385-2"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR604385-2"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR604385-2"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR604385-2"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00794"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR604385-2"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR604385-2"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-80GFKLPL020CC","citationType":"submission","authors":["Ihm H.-S.","Kim S.","Kim S.-M.","Park K.-J.","Lee K.-H."],"publicationDate":"JAN-2003","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO65601.1"}]},{"referenceNumber":2,"citation":{"id":"18713737","citationType":"journal article","authors":["Lee H.J.","Park S.J.","Choi S.H.","Lee K.H."],"citationCrossReferences":[{"database":"PubMed","id":"18713737"},{"database":"DOI","id":"10.1074/jbc.M802219200"}],"title":"Vibrio vulnificus rpoS expression is repressed by direct binding of cAMP-cAMP receptor protein complex to its two promoter regions.","publicationDate":"2008","journal":"J. Biol. Chem.","firstPage":"30438","lastPage":"30450","volume":"283"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO65601.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY221025","properties":[{"key":"ProteinId","value":"AAO65601.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q846Z2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0047631","properties":[{"key":"GoTerm","value":"F:ADP-ribose diphosphatase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0019144","properties":[{"key":"GoTerm","value":"F:ADP-sugar pyrophosphatase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006753","properties":[{"key":"GoTerm","value":"P:nucleoside phosphate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0019693","properties":[{"key":"GoTerm","value":"P:ribose phosphate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd24155","properties":[{"key":"EntryName","value":"NUDIX_ADPRase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.79.10:FF:000011","properties":[{"key":"EntryName","value":"ADP-ribose pyrophosphatase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.79.10","properties":[{"key":"EntryName","value":"Nucleoside Triphosphate Pyrophosphohydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR004385","properties":[{"key":"EntryName","value":"NDP_pyrophosphatase"}]},{"database":"InterPro","id":"IPR015797","properties":[{"key":"EntryName","value":"NUDIX_hydrolase-like_dom_sf"}]},{"database":"InterPro","id":"IPR020084","properties":[{"key":"EntryName","value":"NUDIX_hydrolase_CS"}]},{"database":"InterPro","id":"IPR000086","properties":[{"key":"EntryName","value":"NUDIX_hydrolase_dom"}]},{"database":"NCBIfam","id":"NF008003","properties":[{"key":"EntryName","value":"nudF"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00052","properties":[{"key":"EntryName","value":"nudix-type nucleoside diphosphatase, YffH/AdpP family"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11839:SF5","properties":[{"key":"EntryName","value":"ADP-RIBOSE PYROPHOSPHATASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11839","properties":[{"key":"EntryName","value":"UDP/ADP-SUGAR PYROPHOSPHATASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00293","properties":[{"key":"EntryName","value":"NUDIX"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55811","properties":[{"key":"EntryName","value":"Nudix"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51462","properties":[{"key":"EntryName","value":"NUDIX"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00893","properties":[{"key":"EntryName","value":"NUDIX_BOX"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MQQDDKQHAVFTPEDVEVLSKEPLFQGFFKMIKYRFRHKLFAGGWSNVIEREMFERGHAAAMLPYDPIRGQVVLIEQIRVGALEHQHPWQLEIVAGMIDRDESADDVVRREAQEEAGLEVGQLVSVVSYYPSAGGCSEKLDVFIGEVDASKAHGIHGLDYEDEDIRVHVMTREQAYDLVKQGKIENGASIIALQWLELNHLQLKSQWLEIE","length":211,"molWeight":24099,"crc64":"7ECADAF3A0189412","md5":"6D1725F6C37CF926E0D426532B6DFF3F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Motif":2,"Binding site":4},"uniParcId":"UPI000005F749"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q84HE3","uniProtkbId":"Q84HE3_VIBVL","entryAudit":{"firstPublicDate":"2003-06-01","lastAnnotationUpdateDate":"2025-04-02","lastSequenceUpdateDate":"2003-06-01","entryVersion":66,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM34819.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00019595"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"dTDP-4-dehydrorhamnose 3,5-epimerase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012098"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"5.1.3.13"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Thymidine diphospho-4-keto-rhamnose 3,5-epimerase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM34819.1"}],"value":"rmlC"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001997"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16969"},{"database":"ChEBI","id":"CHEBI:57649"},{"database":"ChEBI","id":"CHEBI:62830"}],"ecNumber":"5.1.3.13","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001298"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"value":"Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Active site","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600888-1"}]},{"type":"Active site","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600888-1"}]},{"type":"Site","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600888-3"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364069"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-CA6RURML6RNLE","citationType":"submission","authors":["Zuppardo A.B.","Siebeling R.J."],"publicationDate":"APR-2002","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM34819.1"}],"value":"1003","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM34819.1"}]},{"referenceNumber":2,"citation":{"id":"12595419","citationType":"journal article","authors":["Smith A.B.","Siebeling R.J."],"citationCrossReferences":[{"database":"PubMed","id":"12595419"},{"database":"DOI","id":"10.1128/IAI.71.3.1091-1097.2003"}],"title":"Identification of genetic loci required for capsular expression in Vibrio vulnificus.","publicationDate":"2003","journal":"Infect. Immun.","firstPage":"1091","lastPage":"1097","volume":"71"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM34819.1"}],"value":"1003","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM34819.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY097060","properties":[{"key":"ProteinId","value":"AAM34819.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_225486226.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_JAERHV010000027.1"}]},{"database":"AlphaFoldDB","id":"Q84HE3","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00124","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0008830","properties":[{"key":"GoTerm","value":"F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019305","properties":[{"key":"GoTerm","value":"P:dTDP-rhamnose biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000271","properties":[{"key":"GoTerm","value":"P:polysaccharide biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd00438","properties":[{"key":"EntryName","value":"cupin_RmlC"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.10","properties":[{"key":"EntryName","value":"Jelly Rolls"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR000888","properties":[{"key":"EntryName","value":"RmlC-like"}]},{"database":"InterPro","id":"IPR014710","properties":[{"key":"EntryName","value":"RmlC-like_jellyroll"}]},{"database":"InterPro","id":"IPR011051","properties":[{"key":"EntryName","value":"RmlC_Cupin_sf"}]},{"database":"NCBIfam","id":"TIGR01221","properties":[{"key":"EntryName","value":"rmlC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21047","properties":[{"key":"EntryName","value":"DTDP-6-DEOXY-D-GLUCOSE-3,5 EPIMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21047:SF2","properties":[{"key":"EntryName","value":"THYMIDINE DIPHOSPHO-4-KETO-RHAMNOSE 3,5-EPIMERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00908","properties":[{"key":"EntryName","value":"dTDP_sugar_isom"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51182","properties":[{"key":"EntryName","value":"RmlC-like cupins"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKVIDTKIPDVKIIEPTVFGDERGFFMETWNQKRFEELVTGKPTKFVQDNHSKSKKGILRGLHYQTENTQGKLVRVVSGEVFDVAVDIRKDSPTFGQWVGEYLSSENKRQLWIPEGFAHGFYVTSEEAEFVYKCTDYYNPTAEVSLRWDDSYVNIIWPCKITPTLSDKDRLGLSWKQFIKTEV","length":183,"molWeight":21285,"crc64":"77BB4118F3F133C5","md5":"E7EBA1F3C88BACB1C1EC3799D1FCD49C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Active site":2,"Site":1},"uniParcId":"UPI0000004AE8"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q84I26","uniProtkbId":"Q84I26_VIBVL","entryAudit":{"firstPublicDate":"2003-06-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2003-06-01","entryVersion":68,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO32663.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"2.7.8.33"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Undecaprenyl-phosphate GlcNAc-1-phosphate transferase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO32663.1"}],"value":"wecA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Catalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP-C55)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl diphosphate + UMP","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:28090"},{"database":"ChEBI","id":"CHEBI:57705"},{"database":"ChEBI","id":"CHEBI:57865"},{"database":"ChEBI","id":"CHEBI:60392"},{"database":"ChEBI","id":"CHEBI:62959"}],"ecNumber":"2.7.8.33","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600715-1"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004651"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004651"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"value":"Belongs to the glycosyltransferase 4 family. WecA subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":156,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":291,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Transmembrane","location":{"start":{"value":317,"modifier":"EXACT"},"end":{"value":337,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}]},{"type":"Region","location":{"start":{"value":352,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":148,"modifier":"EXACT"},"end":{"value":148,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600715-1"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600715-1"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022519"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022475"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022676"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022985"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0448","category":"Biological process","name":"Lipopolysaccharide biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023211"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0464","category":"Ligand","name":"Manganese"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600715-1"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02030"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-CA6RURML6RNLE","citationType":"submission","authors":["Zuppardo A.B.","Siebeling R.J."],"publicationDate":"APR-2002","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO32663.1"}],"value":"1003","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO32663.1"}]},{"referenceNumber":2,"citation":{"id":"12595419","citationType":"journal article","authors":["Smith A.B.","Siebeling R.J."],"citationCrossReferences":[{"database":"PubMed","id":"12595419"},{"database":"DOI","id":"10.1128/IAI.71.3.1091-1097.2003"}],"title":"Identification of genetic loci required for capsular expression in Vibrio vulnificus.","publicationDate":"2003","journal":"Infect. Immun.","firstPage":"1091","lastPage":"1097","volume":"71"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO32663.1"}],"value":"1003","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAO32663.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF499932","properties":[{"key":"ProteinId","value":"AAO32663.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q84I26","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00281","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009276","properties":[{"key":"GoTerm","value":"C:Gram-negative-bacterium-type cell wall"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0030145","properties":[{"key":"GoTerm","value":"F:manganese ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0036380","properties":[{"key":"GoTerm","value":"F:UDP-N-acetylglucosamine-undecaprenyl-phosphate N-acetylglucosaminephosphotransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0044038","properties":[{"key":"GoTerm","value":"P:cell wall macromolecule biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0071555","properties":[{"key":"GoTerm","value":"P:cell wall organization"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009243","properties":[{"key":"GoTerm","value":"P:O antigen biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd06853","properties":[{"key":"EntryName","value":"GT_WecA_like"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_02030","properties":[{"key":"EntryName","value":"WecA_Gammaproteo"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR012750","properties":[{"key":"EntryName","value":"ECA_WecA-rel"}]},{"database":"InterPro","id":"IPR000715","properties":[{"key":"EntryName","value":"Glycosyl_transferase_4"}]},{"database":"NCBIfam","id":"TIGR02380","properties":[{"key":"EntryName","value":"ECA_wecA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR22926","properties":[{"key":"EntryName","value":"PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR22926:SF3","properties":[{"key":"EntryName","value":"UNDECAPRENYL-PHOSPHATE ALPHA-N-ACETYLGLUCOSAMINYL 1-PHOSPHATE TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00953","properties":[{"key":"EntryName","value":"Glycos_transf_4"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFLELSFIFFSSFATLFLMRKVAKRIGLVDKPNARKLHTGSVPLVGGISICLVLAQYLVFRPETINHSWLYLASIVLLTFVGALDDKFDLSFKIRMGIQAALSLVMMHAAGIELHSLGNLFGVGEIYLGWVGSIITIFAVIGAINAFNMVDGIDGLLGGLSIVTFGALAFLLNVDSQHGLAYLCVVIIVAMLPYILMNLGILGRKRKVFMGDAGSMMIGFTVIWLLLGVSQVESKPLMRPVTALWLIAVPLMDMAAIMIRRVRRGDSPFKPDREHLHHIFQRLGLTSRQTLVVICGFATLFAAFGIYGEAANLSEAFMFYSFIACFLVYATLLNYVWRITSLSAAAWEKWRKPTQYKVSSSPPNEARQSKKQK","length":373,"molWeight":41328,"crc64":"BDFDAC1B692A0499","md5":"BDA9A3C5773CBC9E656F42F72A280A0D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":11,"Region":1,"Binding site":2},"uniParcId":"UPI0000003797"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q8VQN5","uniProtkbId":"Q8VQN5_VIBVL","entryAudit":{"firstPublicDate":"2002-03-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2002-03-01","entryVersion":111,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL58393.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"Bifunctional protein PutA"}},"includes":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"Proline dehydrogenase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"1.5.5.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"Proline oxidase"}}]},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"1-pyrroline-5-carboxylate dehydrogenase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"P5C dehydrogenase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"1.2.1.88"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"L-glutamate gamma-semialdehyde dehydrogenase"}}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL58393.1"}],"value":"putA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"Oxidizes proline to glutamate for use as a carbon and nitrogen source"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:30235"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29985"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:58066"}],"ecNumber":"1.2.1.88","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048142"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23784"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17388"},{"database":"ChEBI","id":"CHEBI:24646"},{"database":"ChEBI","id":"CHEBI:60039"},{"database":"ChEBI","id":"CHEBI:132124"}],"ecNumber":"1.5.5.2","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"FAD","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:57692"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004786"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"In the C-terminal section; belongs to the aldehyde dehydrogenase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"value":"In the N-terminal section; belongs to the proline dehydrogenase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Proline utilization A proline dehydrogenase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF18327"}]},{"type":"Domain","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":187,"modifier":"EXACT"}},"description":"Proline dehydrogenase PutA","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF14850"}]},{"type":"Domain","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":490,"modifier":"EXACT"}},"description":"Proline dehydrogenase","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01619"}]},{"type":"Domain","location":{"start":{"value":581,"modifier":"EXACT"},"end":{"value":1036,"modifier":"EXACT"}},"description":"Aldehyde dehydrogenase","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00171"}]},{"type":"Active site","location":{"start":{"value":811,"modifier":"EXACT"},"end":{"value":811,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000197-1"}]},{"type":"Active site","location":{"start":{"value":845,"modifier":"EXACT"},"end":{"value":845,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000197-1"}]},{"type":"Active site","location":{"start":{"value":845,"modifier":"EXACT"},"end":{"value":845,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10008"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"id":"KW-0274","category":"Ligand","name":"FAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023062"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000197"}],"id":"KW-0642","category":"Biological process","name":"Proline metabolism"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-ENPRA4PC40APS","citationType":"submission","authors":["Kim H.J.","Lee J.H.","Choi S.H."],"publicationDate":"DEC-2001","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL58393.1"}]},{"referenceNumber":2,"citation":{"id":"12813078","citationType":"journal article","authors":["Lee J.H.","Park N.Y.","Lee M.H.","Choi S.H."],"citationCrossReferences":[{"database":"PubMed","id":"12813078"},{"database":"DOI","id":"10.1128/JB.185.13.3842-3852.2003"}],"title":"Characterization of the Vibrio vulnificus putAP operon, encoding proline dehydrogenase and proline permease, and its differential expression in response to osmotic stress.","publicationDate":"2003","journal":"J. Bacteriol.","firstPage":"3842","lastPage":"3852","volume":"185"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL58393.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF454004","properties":[{"key":"ProteinId","value":"AAL58393.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_015728452.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_JACGMD010000005.1"}]},{"database":"AlphaFoldDB","id":"Q8VQN5","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAAFMPC","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00261","properties":[{"key":"RectionId","value":"UER00373"}]},{"database":"GO","id":"GO:0009898","properties":[{"key":"GoTerm","value":"C:cytoplasmic side of plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003700","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription factor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003842","properties":[{"key":"GoTerm","value":"F:L-glutamate gamma-semialdehyde dehydrogenase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004657","properties":[{"key":"GoTerm","value":"F:proline dehydrogenase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0055129","properties":[{"key":"GoTerm","value":"P:L-proline biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006562","properties":[{"key":"GoTerm","value":"P:L-proline catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd07125","properties":[{"key":"EntryName","value":"ALDH_PutA-P5CDH"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.309.10:FF:000005","properties":[{"key":"EntryName","value":"1-pyrroline-5-carboxylate dehydrogenase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.220","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.605.10","properties":[{"key":"EntryName","value":"Aldehyde Dehydrogenase, Chain A, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.309.10","properties":[{"key":"EntryName","value":"Aldehyde Dehydrogenase, Chain A, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.5.460","properties":[{"key":"EntryName","value":"Single helix bin"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR016161","properties":[{"key":"EntryName","value":"Ald_DH/histidinol_DH"}]},{"database":"InterPro","id":"IPR016163","properties":[{"key":"EntryName","value":"Ald_DH_C"}]},{"database":"InterPro","id":"IPR016160","properties":[{"key":"EntryName","value":"Ald_DH_CS_CYS"}]},{"database":"InterPro","id":"IPR016162","properties":[{"key":"EntryName","value":"Ald_DH_N"}]},{"database":"InterPro","id":"IPR015590","properties":[{"key":"EntryName","value":"Aldehyde_DH_dom"}]},{"database":"InterPro","id":"IPR025703","properties":[{"key":"EntryName","value":"Bifunct_PutA"}]},{"database":"InterPro","id":"IPR029041","properties":[{"key":"EntryName","value":"FAD-linked_oxidoreductase-like"}]},{"database":"InterPro","id":"IPR041349","properties":[{"key":"EntryName","value":"PRODH"}]},{"database":"InterPro","id":"IPR024089","properties":[{"key":"EntryName","value":"PRODH_PutA_dom_I/II"}]},{"database":"InterPro","id":"IPR024082","properties":[{"key":"EntryName","value":"PRODH_PutA_dom_II"}]},{"database":"InterPro","id":"IPR002872","properties":[{"key":"EntryName","value":"Proline_DH_dom"}]},{"database":"InterPro","id":"IPR050485","properties":[{"key":"EntryName","value":"Proline_metab_enzyme"}]},{"database":"InterPro","id":"IPR005933","properties":[{"key":"EntryName","value":"PutA_C"}]},{"database":"NCBIfam","id":"TIGR01238","properties":[{"key":"EntryName","value":"D1pyr5carbox3"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF008869","properties":[{"key":"EntryName","value":"putA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42862","properties":[{"key":"EntryName","value":"DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42862:SF1","properties":[{"key":"EntryName","value":"DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00171","properties":[{"key":"EntryName","value":"Aldedh"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01619","properties":[{"key":"EntryName","value":"Pro_dh"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14850","properties":[{"key":"EntryName","value":"Pro_dh-DNA_bdg"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18327","properties":[{"key":"EntryName","value":"PRODH"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000197","properties":[{"key":"EntryName","value":"Bifunct_PutA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53720","properties":[{"key":"EntryName","value":"ALDH-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51730","properties":[{"key":"EntryName","value":"FAD-linked oxidoreductase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81935","properties":[{"key":"EntryName","value":"N-terminal domain of bifunctional PutA protein"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00070","properties":[{"key":"EntryName","value":"ALDEHYDE_DEHYDR_CYS"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFTATDVLKPEFVAQPLDKLWALISPLYMVDESQWLEQLLPLATPSAEEKQAIASRTTQLIEAIRADKKSIQLIDALLLEYSLDTHEGILLMCLAEALMRIPDAATADALIKDKMSVADWKSHLKNSESVFVNASTWGLMLTGKVVGLGDDETQSASQALNRLVNKLSEPVIRKAMYQAMKIMGHQFVRGRTIEEAQKNGRPMRDKGFTYSFDMLGEAALTTADANKYFKDYLMAIEAVGRETYGNDTSPAPSVSIKLSALHPRYEVANEHRVMSELFDTLAQLLKRAVELDVAITIDAEEADRLELSLKLFEKLYRSETVKGWGKFGLVVQAYSKRALPVLVWLNALAKEQGDLIPLRLVKGAYWDSEVKWSQQRGYDNYPVYTRKEATDVAYLACARFLLSDDVRGNIFPQFASHNAQTVSSIATMATHSDYEFQRLHGMGDSLYNHVMEQYRQPVRIYAPVGSHKDLLPYLVRRLLENGANSSFVHRLVDARCPVAELTKHPVEALTSFETLNNTKIPLPAAIFPERKNSYGVNVDIESEAKPFEAQVESFLSKQWLAGPIINGASLAESMIKDGQAESVTAPYDRRIQVGQIAFANLDHVSQAIDIAQAAFADWNRTPLVEKARCLDNLADLMEANLAELVALCHQEAGKTLHDSIDEVREAVDFCRYYAKQAQNLQPFELEGFDGVKRIASREGLGVFVCISPWNFPLAIFLGQVTAALVAGNTVVAKPAEQTSLIAVRAVELMLQAGFPASVVQLIPGRGADIGSALTSHPSIAGVAFTGSTATAQRINVTLAQREAKPVPFIAETGGQNAMIVDSTALPEQVVRDVIRSAFASAGQRCSALRVLFVQQDIADRVIGLIQGAMNELSVGVPYLHKTDVGPVIDARAKEKLLQHIEQMNQTQKPIAQLTLADDCQHGDFVTPTAFEISGIDVLKEEQFGPILHIVRFKASELTRVVEHINQTGFGLTMGIHSRNETTYRWIEKHARVGNCYINRDQVGAVVGVQPFGGQGLSGTGPKAGGPHYLYRFTQVQFNQTENA","length":1043,"molWeight":115088,"crc64":"7F79790C22F325A4","md5":"A5CEFD2CEF9ACA8E3422B88F13B7BB66"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"PATHWAY":2,"SIMILARITY":2},"countByFeatureType":{"Domain":4,"Active site":3},"uniParcId":"UPI00000B92D3"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A023NA98","uniProtkbId":"A0A023NA98_VIBVL","entryAudit":{"firstPublicDate":"2014-07-09","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2014-07-09","entryVersion":52,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AHW45240.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AHW45240.1"}],"value":"MARTX"}}]},"comments":[{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004165"}],"value":"Host cell membrane","id":"SL-0375"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023586"}],"value":"Host cytoplasm, host cytosol","id":"SL-0384"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]}],"features":[{"type":"Region","location":{"start":{"value":1610,"modifier":"EXACT"},"end":{"value":1682,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1741,"modifier":"EXACT"},"end":{"value":1895,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":4337,"modifier":"EXACT"},"end":{"value":4363,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":4740,"modifier":"EXACT"},"end":{"value":4781,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1615,"modifier":"EXACT"},"end":{"value":1626,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1627,"modifier":"EXACT"},"end":{"value":1646,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1652,"modifier":"EXACT"},"end":{"value":1671,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1744,"modifier":"EXACT"},"end":{"value":1757,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1778,"modifier":"EXACT"},"end":{"value":1807,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1870,"modifier":"EXACT"},"end":{"value":1880,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":4339,"modifier":"EXACT"},"end":{"value":4354,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":4752,"modifier":"EXACT"},"end":{"value":4764,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7D5Y"},{"evidenceCode":"ECO:0007829","source":"PDB","id":"7P1H"}],"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023315"}],"id":"KW-0012","category":"Molecular function","name":"Acyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022813"}],"id":"KW-0068","category":"PTM","name":"Autocatalytic cleavage"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022511"}],"id":"KW-1032","category":"Cellular component","name":"Host cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023200"}],"id":"KW-1035","category":"Cellular component","name":"Host cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022870"}],"id":"KW-1043","category":"Cellular component","name":"Host membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023121"}],"id":"KW-0446","category":"Ligand","name":"Lipid-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022737"}],"id":"KW-0677","category":"Domain","name":"Repeat"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022807"}],"id":"KW-0788","category":"Molecular function","name":"Thiol protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022656"}],"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023026"}],"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"24614656","citationType":"journal article","authors":["Ziolo K.J.","Jeong H.G.","Kwak J.S.","Yang S.","Lavker R.M.","Satchell K.J."],"citationCrossReferences":[{"database":"PubMed","id":"24614656"},{"database":"DOI","id":"10.1128/IAI.00017-14"}],"title":"Vibrio vulnificus biotype 3 multifunctional autoprocessing RTX toxin is an adenylate cyclase toxin essential for virulence in mice.","publicationDate":"2014","journal":"Infect. Immun.","firstPage":"2148","lastPage":"2157","volume":"82"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AHW45240.1"}],"value":"BAA87","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AHW45240.1"}]},{"referenceNumber":2,"citation":{"id":"CI-HBTHHG3RVIL9","citationType":"submission","authors":["Kim M.-H.","Hwang J.","Choi S."],"title":"Cystein protease domain from MARTX toxin.","publicationDate":"SEP-2020","submissionDatabase":"PDB data bank"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 4091-4300"],"evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7D5Y"}]},{"referenceNumber":3,"citation":{"id":"34785651","citationType":"journal article","authors":["Belyy A.","Merino F.","Mechold U.","Raunser S."],"citationCrossReferences":[{"database":"PubMed","id":"34785651"},{"database":"DOI","id":"10.1038/s41467-021-26889-2"}],"title":"Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens.","publicationDate":"2021","journal":"Nat. Commun.","firstPage":"6628","lastPage":"6628","volume":"12"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (3.90 ANGSTROMS) OF 3229-3658"],"evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7P1H"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KJ131555","properties":[{"key":"ProteinId","value":"AHW45240.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_039507922.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP041683.1"}]},{"database":"PDB","id":"7D5Y","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"A=4091-4300"}]},{"database":"PDB","id":"7P1H","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.90 A"},{"key":"Chains","value":"A=3229-3658"}]},{"database":"SMR","id":"A0A023NA98","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|672.127.peg.4167","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"PHI-base","id":"PHI:3354","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0044164","properties":[{"key":"GoTerm","value":"C:host cell cytosol"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0020002","properties":[{"key":"GoTerm","value":"C:host cell plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008294","properties":[{"key":"GoTerm","value":"F:calcium- and calmodulin-responsive adenylate cyclase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004197","properties":[{"key":"GoTerm","value":"F:cysteine-type endopeptidase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd20494","properties":[{"key":"EntryName","value":"C58_RtxA"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd20501","properties":[{"key":"EntryName","value":"C80_RtxA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd21058","properties":[{"key":"EntryName","value":"toxin_MLD_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.140.180","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.11050","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1820","properties":[{"key":"EntryName","value":"alpha/beta hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.1760.10","properties":[{"key":"EntryName","value":"Anthrax toxin, edema factor, central domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.260","properties":[{"key":"EntryName","value":"Galactose-binding domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR000073","properties":[{"key":"EntryName","value":"AB_hydrolase_1"}]},{"database":"InterPro","id":"IPR029058","properties":[{"key":"EntryName","value":"AB_hydrolase_fold"}]},{"database":"InterPro","id":"IPR035099","properties":[{"key":"EntryName","value":"Anthrax_toxin_C-terminal"}]},{"database":"InterPro","id":"IPR005165","properties":[{"key":"EntryName","value":"Anthrax_toxin_edema_cen"}]},{"database":"InterPro","id":"IPR037017","properties":[{"key":"EntryName","value":"Anthrax_toxin_edema_cen_sf"}]},{"database":"InterPro","id":"IPR020974","properties":[{"key":"EntryName","value":"CPD_dom"}]},{"database":"InterPro","id":"IPR038383","properties":[{"key":"EntryName","value":"CPD_dom_sf"}]},{"database":"InterPro","id":"IPR020972","properties":[{"key":"EntryName","value":"Dermonecrotic/RTX_toxin_MLD"}]},{"database":"InterPro","id":"IPR059883","properties":[{"key":"EntryName","value":"IcsB/BopA_M"}]},{"database":"InterPro","id":"IPR061305","properties":[{"key":"EntryName","value":"MARTX_Nterm"}]},{"database":"InterPro","id":"IPR006473","properties":[{"key":"EntryName","value":"Peptidase_C58_Yopt"}]},{"database":"InterPro","id":"IPR049824","properties":[{"key":"EntryName","value":"RtxA-like_C80"}]},{"database":"InterPro","id":"IPR048568","properties":[{"key":"EntryName","value":"RtxA_C"}]},{"database":"InterPro","id":"IPR011509","properties":[{"key":"EntryName","value":"RtxA_toxin"}]},{"database":"InterPro","id":"IPR011049","properties":[{"key":"EntryName","value":"Serralysin-like_metalloprot_C"}]},{"database":"NCBIfam","id":"NF012221","properties":[{"key":"EntryName","value":"MARTX_Nterm"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12277","properties":[{"key":"EntryName","value":"ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12277:SF81","properties":[{"key":"EntryName","value":"PROTEIN ABHD13"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00561","properties":[{"key":"EntryName","value":"Abhydrolase_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03497","properties":[{"key":"EntryName","value":"Anthrax_toxA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF27349","properties":[{"key":"EntryName","value":"IcsB_M"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11647","properties":[{"key":"EntryName","value":"MLD"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03543","properties":[{"key":"EntryName","value":"Peptidase_C58"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11713","properties":[{"key":"EntryName","value":"Peptidase_C80"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07634","properties":[{"key":"EntryName","value":"RtxA"},{"key":"MatchStatus","value":"41"}]},{"database":"Pfam","id":"PF21735","properties":[{"key":"EntryName","value":"RtxA_C"},{"key":"MatchStatus","value":"6"}]},{"database":"SUPFAM","id":"SSF81298","properties":[{"key":"EntryName","value":"Adenylylcyclase toxin (the edema factor)"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53474","properties":[{"key":"EntryName","value":"alpha/beta-Hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51120","properties":[{"key":"EntryName","value":"beta-Roll"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF158842","properties":[{"key":"EntryName","value":"PMT central region-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51771","properties":[{"key":"EntryName","value":"CGT_MARTX_CPD"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGKPFWRSVEYFFTGNYSADDGNNSIVAIGFGGEIHAYGGDDHVTVGSIGAKVYTGSGNDTVVGGSAYLRVEDTTGHLSVKGAAGYADINKSGDGNVSFAGAAGGVSIDHLGNHGDVNYGGAAAYNGITRKGLSGNVTFKGAGGYNALWHETNQGNLSFAGAGAGNKLDRTWFNRYQGSRGDVTFDGAGAANSISSRVETGNITFRGAGADNHLVRKGKVGDITLQGAGASNRIERTRQAEDVYAQTRGNIRFEGVGGYNSLYSDVAHGDIHFSGGGAYNTITRKGSGSSFDAQGMEYAKAEEIVLTAAQMHGLSIDNGNKFHAVTAVKSEREPNTYLFAIADGTYTKINKVRLYNDPETGKLKYYSEAWFKRGNHLAELARSDVSSAGGFEVNPINGGYTLSNIAVEHQQSLTVHAVEKDLTEYEWVTYANGVLIDAKDVALSEAKMGGHAISTDGTTVDVQAVKSNRKPNTYVYAKVLGPYTKIVVVELANDPKTGALKYQARSWYKEGNHTANLANEDISSANGYHSMGKSGYSLSDLHYSVNAVRSTSETVADIDEYTDQTLFKPATDSGESSGDVRFNGAGGGNVIKSNVTRGNVYFNGGGIANVILHSSQFGHTEFNGGGAANVIVKSGEEGDLTFRGAGLANVLVHQSKQGKMDVYAGGAVNVLVRIGDGQYLAHLLAYGNISVHKGNGNSRVVMLGGYNTHTQIGSGNGLWLAAGGFNVMTQVGKGDVASVLAGGANVLTKVGDGDLTAGMLGGANVITHISSDNETSNTTAVALGGANILTKKGKGNTVAVMGGGANVLTHVGDGTTTGVMVGGANILTKVGNGDTTGIMLGVGNVLTHVGDGQTLGVMGAAGNIFTKVGDGTSIAVMIGAGNIFTHVGEGNAWALMGGLGNVFTKVGNGDALALMVAEANVFTHIGDGMSVALMLAKGNVATKVGNGTTLAAMVGNANIFTHLGSGSTFAAMIGQANIMTKVGNDLTAALMVGKANIYTHVGDGTSLGIFAGEVNVMTKVGNGTTLAAMFGKANIMTHVGDGLTGVLALGEANIVTKVGDDFMGVVAAAKANVVTHVGDATTAAVLAGKGNILTKVGEGTTVGLLISDIGNVMTHVGDGTTIGIAKGKANIITKVGDGLGVNVAWGQANVFTQVGDGDRYNFAKGEANIITKVGDGQEVSVVQGKANIITHVGNGDDYTGAWGKANVITKVGDGRNVVLAKGEANIVTQVGDGDSFNALWSKGNIVTKVGDGMQVTAAKGKANITTTVGDGLSVTAAYGDANINTKVGDGVSVNVAWGKYNINTKVGDGLNVAVMKGKANANIHVGDGLNINASYAQNNVAIKVGNGDFYSLAVASSNTSSNKLSALFDNIKQTLLGVGGSQAINYLVQGDEASSSGTQKGRGAIATPEITKLDGFQMEAIEEVGSDLGDSLTGSVTKVDTPDLNKVQNALDVDGSSDQTQAPNLIVNGDFEQGDQGWKSTHGVEASHSGNVYGVNGEGHGARVTELDTYTNTSLYQDLTDLTEGEVIAVSFDFAKRAGLSNNEGIEVLWNGEVVFSSSGDASAWQQKTLKLTAHAGSNRIEFKGTGHNDGLGYILDNVVAKSESSLQAKAVSEHATQNQASQNALSDKERAEADRQRLEQEKQKQLDAVAGSQSQLESTDQQALENNGQAQRDAVKEESEAVTAELTKLAQGLDVLDGQATHTGESGDQWRNDFAGGLLDGVQRQLDDAKQLANDKIAAAKQTHADNQNKVKDAVAKSEAGVAKGEQNRAGAEQDIADAKADAEKRKADALAKGKDAQQAESDAHHAVNNAQSRGERDVQLAENKANQAQADAQGAKQNEGDRPDRQGVAGSGLSGNAHSVEGAGETGSHVNADSSTNADGRFSEGLSEQEQEALEGATNAVNRLQINAGIRGKNSGSTITSMFTETNSDSIVVPTTASQDVVRKEIRISGVNLEGLGEASHDSAESLVAARAEKVANLYRWLDTENDVATDKYVPVPGFERVDADVSDEVKQRMIQSMSGYIEHTDNQVPKDQAEALATLFVESTLDYDWDKRVEFLTKLESYGYSFEAPHAEKSIVSFWSGKNFKQYRDVLDNAQTDGKKVVYDIDVKGNAFAMDLNKHLMRWGGLFLDPDNAEQNQLKSSIDAATFSNTGFWSSVYATGAQNDVYVIAEGGVRLGNYFWNVDLPALRQLQREGLVGEIRLLDKPVSEYKDLPADQIGRRLTDAGVAVKVRFDALSHERQAELLADNPDGYKADTLVELDVKLSAIDSMLRESLPFYSLRTERNLLVQEGEEGFEVRSWPGTDGKSKTILLDNPEDAAQQKSIERFILANFDNFEQMPDELFLVDNKVLSHHDGRTRILAQKEDGAWTYNTNVELMSVTELLDAAHVSGKVRGESYQQVIDALTEYHASTAEHADYELTSVEKLLNLRKQVEGYVLGHPDSGRVQAMNSLLNQVNSRLEAVSVLVVSEQSIKAHDSFSHLYDQLDNANLKESKHLYLDGNGDFVTKGKGNLANIDKLGGSDAVLEKVKAAVTHEYGQVVADTIFAGLSANDLAKDGKGIDIAGLNKVHQAIEQHMSPVSATMYIWKPSKHSTLGHAALQIGQGRTQLEGQAAADFNKQNYVSWWPLGSKSPNIGNILNVATKDQPDLKLRWSDFSQPAHQSDTLEHDMAAEENDSFGLKKGEAKLKRFIEELNAAKGIDASFKMASEGYASLLLGNPDMLASTGIPAHVFQPFLDQWNDTSYDMMDVANRFAEELQKQAKIEVNPEQIEQQISEVVKEFAQDELDKIQAFKVAQADQGRVFRINLEGLDVAAMQAEWHRLSNDPDARYQLLTKNCSSTVAKVLKAGGADKLIGHTWLPKFGVWTPTELFNFGQALQEAQLEIAAKKQSHQVTDVLDALSGNEKHKENVAIENDGTPPRDKESLSPLTRFLNNELYGEKDARRKIGEITQTLLDHAVENGESQKVTLKGEAGRLTGYYHQGAASSEGETSATSGKVVLFLHGSGSSAEEQASEIRNHYQKQGIDMLAVNLRGYGESDGGPSEKGLYQDARTMFNYLVNDKGIDPSNIIIHGYSMGGPIAADLARYAAQNGQAVSGLLLDRPMPSMTKAITAHEVANPAGIVGAIAKAVNGQFSVEKNLKGLPKETPILLLTDNEGLGEEGEKLRAKLAIAGYNVSGEQTFYGHEASNRLMGQYADQIVSGLFNAEQAAVEVKDIRATEDLSVVKTVASDTELGTNTDAPHKNYQSRDLVLEPIVQPETIELGMPDSDQKILAEVAERENVIIGVRPVDEKSKSLIDSKLYSSKGLFVKAKSSDWGPMSGFIPVDQAFAKASARRDLDKFNGYAEQSIESGNAVSADLYLNQVRIDELVSKYQSLTALEFDAESGMYKTTATNGDQTVTFFLNKVTVDSKDLWQVHYIKDGKLAPFKVIGDPVSKQPMTADYDLLTVMYSYSDLGPQDKLKQPLTWEQWKESVTYEELTPKYKELYNSEVLYNKKDGASLGVVSDRLKALKDVINTSLGRTDGLEMVHHGADDANPYAVMADNFPATFFVPKSFFMEDGLGEGKGSIQTYFNVNEQGAVVIRDPQEFSNFQQVAINVSYRASLNDKWNVGLDDPLFTPKSKLSHDFLNAKEEVIKKLSGEVETNVRTTQLLTDNEGLGNEGEKLRTKPTASGFFESSDVQPEVMKGLLQNVGDKIFDLKAGGKELDMFSFHFSQSADLLNKLITLIPEVGNVLITNGNDVQSKDFLEGVCFALSARYMMEERVHGLGGGKAYMEWLKDTVQAYNDNITNKKNDIGSVEQKLLNQYRRQNLGLAIKDLLSMQYSQLMDTSTSAARDAANKEYSGKLRANGLTGPNINDALNYGADGYESVMDKLRNVNKSTYMTFMSQGHAMSVVVHKKGNHKVWSFYDPNFGTKSFAQYDDFRGFMDNFHKGLLTQYKFQDSEEAGQSFYVRFKKFEEGDISSYDGLWKNAREGEQSYVLRALKEQGKTFSMGKNITGKLVDFNDDVITLDVTSKNGRKVLVEVAVSDISQAANLVKTNISQVFSDPLASKLSIQSHAESATITVLEVSGQEAISEVVEGAKIPGQKDAWTGATSKADNQNVNDWERVVVTPAVDGGETRFDGQIIVQMENDAVAAKAAANLAGKHPESSVVVQLDSDGNYRVVYGDPSKLDGKIRWQLVGHGRDHSESNNTRLSGYSADELAVKLASFQQMFNQAEKISSKPDHISIVGCSLVSDDKQKGFGHQFINAMDANGLRVDVSVRSAKVYINEMGRKLYFDGKDSWVNKAINSKVLLSWNGQGDVVAKDERIRNGIAEGDIDLSRIGISDVDEPARGAIGDNKDVFDAPEKRKAETETSSSSANNKLSYSGNIQVNVGDGEFTAVNWGTSNVGIKVGTGGFKSLAFGDNNVMVHIGNGESKHSFDIGGYQALEGAQMFIGNRNVSFNKGRSNDLIVMMDKSIPTPPLVNPFDGAARISGVLQSIATSGEGQDWLAAQEQQWTLSGAKKFVKDMSGLDQSSSVDYTSLVELDSQNERSSRGLKHDAEAALNKQYNQWLSGNGDSDTSKLSRADKLRQANEKLAFNFAVGGQGADIQVTTGNWNFMFGDNIQSILDTNLGSLFGLMTQQFSATGQAKTTFTYTPEDLPRQLKNKLLGQLAGVGAETTLADIFGVDYTASGQIVSRNGEAVDGVSILKEMLEVIGEFSGDQLQAFVDPAKLLDSLKSGINMGADGIKSFAETHGLKEKAPEEEEDNSSVSVNGASVNSAQGATVADGSTETAETPDRAFGFNSLNLPNLFATIFSQDKQKEMKSLVENLKENLTADLLNMKEKTFDFLRNSGHLQGDGDINISLGNYNFNWGGDGKDLGAYLGDNNNFWGGRGDDVFYATGTSNIFTGGEGNDMGVLMGRENMMFGGDGNDTAVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTSGNFNRVDTGDDQDYSVTIGNNNQVELGAGNDFANVFGNYNRINASAGNDVVKLMGYHAVLNGGEGEDHLIAAAISKFSQFNGGEGRDLMVLGGYQNTFKGGTDVDSFVVSGDVIDNLVEDIRSEDNIVFNGIDWQKLWFERSGYDLKLSILRDPASDSDQAKFEHIGSVTFSDYFNGNRAQVIIAMGEKDATGEREYTTLSESAIDALVQAMSGFDPQAGDNGFIDNLDSKSRVAITTAWADVVHKKGITV","length":5208,"molWeight":557974,"crc64":"3C2EDBF9636F647C","md5":"917235BF48498F9C306B2F276B687719"},"extraAttributes":{"countByCommentType":{"COFACTOR":1,"SUBCELLULAR LOCATION":1},"countByFeatureType":{"Region":4,"Compositional bias":8},"uniParcId":"UPI00044863C1"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V8MTS1","uniProtkbId":"A0A1V8MTS1_VIBVL","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2017-06-07","entryVersion":50,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB49431.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"value":"DNA-directed RNA polymerase subunit beta"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"}],"value":"RNAP subunit beta"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"value":"2.7.7.6"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"}],"value":"RNA polymerase subunit beta"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"}],"value":"Transcriptase subunit beta"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB49431.1"}],"value":"rpoB"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB49431.1"}],"value":"CRN52_04435"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AXX58476.1"}],"value":"FORC53_0137"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"value":"DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21248"},{"database":"Rhea","id":"RHEA-COMP:14527"},{"database":"Rhea","id":"RHEA-COMP:17342"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:61557"},{"database":"ChEBI","id":"CHEBI:140395"}],"ecNumber":"2.7.7.6","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048552"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"value":"The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000434"}],"value":"Belongs to the RNA polymerase beta chain family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":499,"modifier":"EXACT"}},"description":"RNA polymerase beta subunit protrusion","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04563"}]},{"type":"Domain","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":226,"modifier":"EXACT"}},"description":"RNA polymerase Rpb2","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04561"}]},{"type":"Domain","location":{"start":{"value":339,"modifier":"EXACT"},"end":{"value":454,"modifier":"EXACT"}},"description":"RNA polymerase Rpb2","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04561"}]},{"type":"Domain","location":{"start":{"value":513,"modifier":"EXACT"},"end":{"value":580,"modifier":"EXACT"}},"description":"RNA polymerase Rpb2","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04565"}]},{"type":"Domain","location":{"start":{"value":591,"modifier":"EXACT"},"end":{"value":656,"modifier":"EXACT"}},"description":"DNA-directed RNA polymerase beta subunit external 1","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF10385"}]},{"type":"Domain","location":{"start":{"value":717,"modifier":"EXACT"},"end":{"value":1263,"modifier":"EXACT"}},"description":"DNA-directed RNA polymerase subunit 2 hybrid-binding","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00562"}]},{"type":"Domain","location":{"start":{"value":1265,"modifier":"EXACT"},"end":{"value":1340,"modifier":"EXACT"}},"description":"RNA polymerase Rpb2","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04560"}]},{"type":"Coiled coil","location":{"start":{"value":936,"modifier":"EXACT"},"end":{"value":963,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Coils"}]},{"type":"Coiled coil","location":{"start":{"value":1004,"modifier":"EXACT"},"end":{"value":1031,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Coils"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Coils"}],"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022478"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"}],"id":"KW-0240","category":"Cellular component","name":"DNA-directed RNA polymerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022695"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"}],"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023163"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"}],"id":"KW-0804","category":"Biological process","name":"Transcription"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01321"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-6EHGQ51VR50SL","citationType":"submission","authoringGroup":["Food-borne Pathogen Omics Research Center"],"authors":["Chung H.Y.","Na E.J.","Song J.S.","Kim H.","Lee J.-H.","Ryu S.","Choi S.H."],"title":"Complete Genome Sequence of Vibrio vulnificus FORC_053.","publicationDate":"JAN-2017","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AXX58476.1"}],"value":"FORC_053","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AXX58476.1"}]},{"referenceNumber":2,"citation":{"id":"29358930","citationType":"journal article","authors":["Roig F.J.","Gonzalez-Candelas F.","Sanjuan E.","Fouz B.","Feil E.J.","Llorens C.","Baker-Austin C.","Oliver J.D.","Danin-Poleg Y.","Gibas C.J.","Kashi Y.","Gulig P.A.","Morrison S.S.","Amaro C."],"citationCrossReferences":[{"database":"PubMed","id":"29358930"}],"title":"Phylogeny of Vibrio vulnificus from the Analysis of the Core-Genome: Implications for Intra-Species Taxonomy.","publicationDate":"2018","journal":"Front. Microbiol.","firstPage":"2613","lastPage":"2613","volume":"8"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB49431.1"}],"value":"CECT4608","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"POB49431.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP019290","properties":[{"key":"ProteinId","value":"AXX58476.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"PDGH01000036","properties":[{"key":"ProteinId","value":"POB49431.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_011079201.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_AP026552.1"}]},{"database":"SMR","id":"A0A1V8MTS1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAANLDC","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003899","properties":[{"key":"GoTerm","value":"F:DNA-directed RNA polymerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0032549","properties":[{"key":"GoTerm","value":"F:ribonucleoside binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006351","properties":[{"key":"GoTerm","value":"P:DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00653","properties":[{"key":"EntryName","value":"RNA_pol_B_RPB2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.30.150.10:FF:000001","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.270.10:FF:000003","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.270.10:FF:000004","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.50.100:FF:000006","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.50.150:FF:000001","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.1100.10:FF:000002","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.1800.10:FF:000001","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.100","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.150","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.1100.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"6.10.140.1670","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.30.150.10","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase, beta subunit, external 1 domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.270.10","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase, subunit 2, domain 6"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.1800.10","properties":[{"key":"EntryName","value":"RNA polymerase alpha subunit dimerisation domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01321","properties":[{"key":"EntryName","value":"RNApol_bact_RpoB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR042107","properties":[{"key":"EntryName","value":"DNA-dir_RNA_pol_bsu_ext_1_sf"}]},{"database":"InterPro","id":"IPR019462","properties":[{"key":"EntryName","value":"DNA-dir_RNA_pol_bsu_external_1"}]},{"database":"InterPro","id":"IPR015712","properties":[{"key":"EntryName","value":"DNA-dir_RNA_pol_su2"}]},{"database":"InterPro","id":"IPR007120","properties":[{"key":"EntryName","value":"DNA-dir_RNAP_su2_dom"}]},{"database":"InterPro","id":"IPR037033","properties":[{"key":"EntryName","value":"DNA-dir_RNAP_su2_hyb_sf"}]},{"database":"InterPro","id":"IPR010243","properties":[{"key":"EntryName","value":"RNA_pol_bsu_bac"}]},{"database":"InterPro","id":"IPR007121","properties":[{"key":"EntryName","value":"RNA_pol_bsu_CS"}]},{"database":"InterPro","id":"IPR007644","properties":[{"key":"EntryName","value":"RNA_pol_bsu_protrusion"}]},{"database":"InterPro","id":"IPR007642","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_2"}]},{"database":"InterPro","id":"IPR007645","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_3"}]},{"database":"InterPro","id":"IPR007641","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_7"}]},{"database":"InterPro","id":"IPR014724","properties":[{"key":"EntryName","value":"RNA_pol_RPB2_OB-fold"}]},{"database":"NCBIfam","id":"NF001616","properties":[{"key":"EntryName","value":"rpoB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR02013","properties":[{"key":"EntryName","value":"rpoB"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR20856","properties":[{"key":"EntryName","value":"DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04563","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04561","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_2"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF04565","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF10385","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_45"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00562","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_6"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04560","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_7"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF64484","properties":[{"key":"EntryName","value":"beta and beta-prime subunits of DNA dependent RNA-polymerase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01166","properties":[{"key":"EntryName","value":"RNA_POL_BETA"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVYSYTEKKRIRKDFGTRPQVLDIPYLLSIQLDSFDKFIEQDPEGQYGLEAAFRSVFPIQSYNGNSELQYVSYRLGEPVFDVKECQIRGVTYSKPLRVKLRLVIFDKDAPAGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLYVRIDRRRKLPSTIILRALGKSTEEILDTFFEKVNFEVKDQTLMMELVPDRLRGETATFDIEANGTVYVEKGRRVTARHIRQLEKEGVDQIEVPVEYIVGKVSSKDYINEATGEIIVAANQEISLEALAKLSQAGHKQLEVLFTNDLDHGPFMSETLRIDSSVDRISALVEIYRMMRPGEPPTKEAAEALFESLFFSEERYDLSTVGRMKFNSSIGRDDAEEQGTLDETDIIEVMKKLIAIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDAVMPQDLINAKPISAAVKEFFGSSQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHVTHYGRLCPIETPEGPNIGLINSLSAFARCNEYGFLETPYRRVVDGVVTDEVDYLSAIEEGQFVIAQANAKLNEDGTFADELITARQKGESGLHPREHVDYMDVATNQVVSIAASLIPFLEHDDANRALMGANMQRQAVPTLKAEKPLVGTGIERNVAVDSGVTSVAKRGGIIQSVDASRIVVKVNEEELIPGEAGIDIYNLTKYTRSNQNTCINQRPCVMPGEPVLRGDVLADGPSTDLGELALGQNMRIAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELTCVARDTKLGSEEITADIPNVGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPNSVSGTIIDVQVFTRDGVEKDKRALEIEQMQLKEAKKDLTEEFQILEGGLLNRVKAVLLSGGYSEAKLDTTDRKKWLELTLEDDALQTQLEQLAEQYDELKADFDKKFETKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPVEDMPYDEKGQPVDIVLNPLGVPSRMNIGQILEVHLGLAAKGIGDKINQMVKEQQELAKFREFLQKVYDLGETRQKVDIASLSDEEVRTLIGNLRGGLPIATPVFDGASEASIKELLKLGGLPESGQLTLFDGRTGDAFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHAMEPGMPESFNVLLKEIRSLGINIELEDEQ","length":1342,"molWeight":149619,"crc64":"33D873C489AFC126","md5":"0BB20C9BD7922B3D10380FDFFDD7E904"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Domain":7,"Coiled coil":2},"uniParcId":"UPI0000058BA6"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1W6AWN9","uniProtkbId":"A0A1W6AWN9_VIBVL","entryAudit":{"firstPublicDate":"2017-07-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2017-07-05","entryVersion":32,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35836.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00016403"}],"value":"Uridylate kinase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012899"}],"value":"2.7.4.22"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00032092"}],"value":"Uridine monophosphate kinase"}}],"flag":"Fragment"},"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"UMP + ATP = UDP + ADP","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24400"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:57865"},{"database":"ChEBI","id":"CHEBI:58223"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.4.22","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047767"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004791"}],"value":"Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007614"}],"value":"Belongs to the UMP kinase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"Aspartate/glutamate/uridylate kinase","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00696"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35836.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35836.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022777"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35836.1"}],"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022975"}],"id":"KW-0665","category":"Biological process","name":"Pyrimidine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28399796","citationType":"journal article","authors":["Wendling C.C.","Piecyk A.","Refardt D.","Chibani C.","Hertel R.","Liesegang H.","Bunk B.","Overmann J.","Roth O."],"citationCrossReferences":[{"database":"PubMed","id":"28399796"},{"database":"DOI","id":"10.1186/s12862-017-0930-2"}],"title":"Tripartite species interaction: eukaryotic hosts suffer more from phage susceptible than from phage resistant bacteria.","publicationDate":"2017","journal":"BMC Evol. Biol.","firstPage":"98","lastPage":"98","volume":"17"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35836.1"}],"value":"K10K1","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35836.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY771187","properties":[{"key":"ProteinId","value":"ARJ35836.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1W6AWN9","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00159","properties":[{"key":"RectionId","value":"UER00275"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0033862","properties":[{"key":"GoTerm","value":"F:UMP kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0044210","properties":[{"key":"GoTerm","value":"P:'de novo' CTP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"GO","id":"GO:0006225","properties":[{"key":"GoTerm","value":"P:UDP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd04254","properties":[{"key":"EntryName","value":"AAK_UMPK-PyrH-Ec"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.1160.10:FF:000001","properties":[{"key":"EntryName","value":"Uridylate kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1160.10","properties":[{"key":"EntryName","value":"Acetylglutamate kinase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01220_B","properties":[{"key":"EntryName","value":"PyrH_B"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036393","properties":[{"key":"EntryName","value":"AceGlu_kinase-like_sf"}]},{"database":"InterPro","id":"IPR001048","properties":[{"key":"EntryName","value":"Asp/Glu/Uridylate_kinase"}]},{"database":"InterPro","id":"IPR011817","properties":[{"key":"EntryName","value":"Uridylate_kinase"}]},{"database":"InterPro","id":"IPR015963","properties":[{"key":"EntryName","value":"Uridylate_kinase_bac"}]},{"database":"NCBIfam","id":"TIGR02075","properties":[{"key":"EntryName","value":"pyrH_bact"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42833","properties":[{"key":"EntryName","value":"URIDYLATE KINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42833:SF4","properties":[{"key":"EntryName","value":"URIDYLATE KINASE PUMPKIN, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00696","properties":[{"key":"EntryName","value":"AA_kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF005650","properties":[{"key":"EntryName","value":"Uridylate_kin"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53633","properties":[{"key":"EntryName","value":"Carbamate kinase-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"PKPAYQRILLKLSGEALQGEDGFGIDPAVLDRMAQEVKELVELGVQVGVVIGGGNLFRGAGLAEAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNARVMSAIPLKGVCDDYNWADAIRELRQGRVVIFSAGTGNPFFTTDSAACLRGIEIEADVVLKATKVDGVFTADPVANPDAELYDKLSYAEVLDKELKVM","length":196,"molWeight":20995,"crc64":"63E358220559DD0F","md5":"F3D1BC6EF34EB2EC8C1F969EE15414CA"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI000A179A5A"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1W6AWZ3","uniProtkbId":"A0A1W6AWZ3_VIBVL","entryAudit":{"firstPublicDate":"2017-07-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2017-07-05","entryVersion":32,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Vibrio vulnificus","taxonId":672,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35935.1"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Vibrionales","Vibrionaceae","Vibrio"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00015553"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000526"}],"value":"Protein RecA"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000526"}],"value":"Recombinase A"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35935.1"}],"value":"recA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000526"}],"value":"Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009391"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004527"}],"value":"Belongs to the RecA family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"RecA family profile 1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50162"}]},{"type":"Domain","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"RecA family profile 2","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50163"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35935.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35935.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004527"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022763"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004527"}],"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023172"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004527"}],"id":"KW-0233","category":"Biological process","name":"DNA recombination"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023204"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000526"}],"id":"KW-0234","category":"Biological process","name":"DNA repair"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023125"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004527"}],"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004527"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023236"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000526"}],"id":"KW-0742","category":"Biological process","name":"SOS response"}],"references":[{"referenceNumber":1,"citation":{"id":"28399796","citationType":"journal article","authors":["Wendling C.C.","Piecyk A.","Refardt D.","Chibani C.","Hertel R.","Liesegang H.","Bunk B.","Overmann J.","Roth O."],"citationCrossReferences":[{"database":"PubMed","id":"28399796"},{"database":"DOI","id":"10.1186/s12862-017-0930-2"}],"title":"Tripartite species interaction: eukaryotic hosts suffer more from phage susceptible than from phage resistant bacteria.","publicationDate":"2017","journal":"BMC Evol. Biol.","firstPage":"98","lastPage":"98","volume":"17"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35935.1"}],"value":"K10K1","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARJ35935.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY771286","properties":[{"key":"ProteinId","value":"ARJ35935.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1W6AWZ3","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0140664","properties":[{"key":"GoTerm","value":"F:ATP-dependent DNA damage sensor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003697","properties":[{"key":"GoTerm","value":"F:single-stranded DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006281","properties":[{"key":"GoTerm","value":"P:DNA repair"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00983","properties":[{"key":"EntryName","value":"RecA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000087","properties":[{"key":"EntryName","value":"Recombinase RecA"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003593","properties":[{"key":"EntryName","value":"AAA+_ATPase"}]},{"database":"InterPro","id":"IPR013765","properties":[{"key":"EntryName","value":"DNA_recomb/repair_RecA"}]},{"database":"InterPro","id":"IPR020584","properties":[{"key":"EntryName","value":"DNA_recomb/repair_RecA_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR049428","properties":[{"key":"EntryName","value":"RecA-like_N"}]},{"database":"InterPro","id":"IPR020588","properties":[{"key":"EntryName","value":"RecA_ATP-bd"}]},{"database":"InterPro","id":"IPR020587","properties":[{"key":"EntryName","value":"RecA_monomer-monomer_interface"}]},{"database":"NCBIfam","id":"TIGR02012","properties":[{"key":"EntryName","value":"tigrfam_recA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45900:SF1","properties":[{"key":"EntryName","value":"MITOCHONDRIAL DNA REPAIR PROTEIN RECA HOMOLOG-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45900","properties":[{"key":"EntryName","value":"RECA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00154","properties":[{"key":"EntryName","value":"RecA_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00142","properties":[{"key":"EntryName","value":"RECA"}]},{"database":"SMART","id":"SM00382","properties":[{"key":"EntryName","value":"AAA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00321","properties":[{"key":"EntryName","value":"RECA_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50162","properties":[{"key":"EntryName","value":"RECA_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50163","properties":[{"key":"EntryName","value":"RECA_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"TISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVYAKKLGVDIDALLVSQPDTGEQALEICDALARSGAIDVMVVDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGNLKQSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGDEVVGNETRIKVVKNKIAAPFKEANTQIMYGQGFNREG","length":234,"molWeight":24938,"crc64":"4C3FB5E8C1C1AFD3","md5":"5AC90F75F20C9D9CF9768B889BFE4E11"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Non-terminal residue":2},"uniParcId":"UPI000A17B279"}}]}