{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFP9","uniProtkbId":"A0A1V0QFP9_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2017-06-07","entryVersion":28,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67150.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012973"}],"value":"2-isopropylmalate synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012973"}],"value":"2.3.3.13"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67150.1"}],"value":"IPMS"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00059544"}],"value":"Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Involved in Leu biosynthesis, but does not participate in the chain elongation of glucosinolates"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21524"},{"database":"ChEBI","id":"CHEBI:1178"},{"database":"ChEBI","id":"CHEBI:11851"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"}],"ecNumber":"2.3.3.13","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000064"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004689"}],"value":"Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009396"}],"value":"Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":357,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50991"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022605"}],"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023304"}],"id":"KW-0100","category":"Biological process","name":"Branched-chain amino acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022430"}],"id":"KW-0432","category":"Biological process","name":"Leucine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67150.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY618808","properties":[{"key":"ProteinId","value":"ARE67150.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFP9","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00048","properties":[{"key":"RectionId","value":"UER00070"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"CDD","id":"cd07940","properties":[{"key":"EntryName","value":"DRE_TIM_IPMS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000010","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.238.260:FF:000003","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase 1 chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.160.270:FF:000004","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase B"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.238.260","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.160.270","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01025","properties":[{"key":"EntryName","value":"LeuA_type1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR013709","properties":[{"key":"EntryName","value":"2-isopropylmalate_synth_dimer"}]},{"database":"InterPro","id":"IPR002034","properties":[{"key":"EntryName","value":"AIPM/Hcit_synth_CS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR054691","properties":[{"key":"EntryName","value":"LeuA/HCS_post-cat"}]},{"database":"InterPro","id":"IPR036230","properties":[{"key":"EntryName","value":"LeuA_allosteric_dom_sf"}]},{"database":"InterPro","id":"IPR005671","properties":[{"key":"EntryName","value":"LeuA_bact_synth"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"NCBIfam","id":"TIGR00973","properties":[{"key":"EntryName","value":"leuA_bact"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF002086","properties":[{"key":"EntryName","value":"PRK00915.1-3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277:SF9","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22617","properties":[{"key":"EntryName","value":"HCS_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08502","properties":[{"key":"EntryName","value":"LeuA_dimer"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00917","properties":[{"key":"EntryName","value":"LeuA_dimer"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF110921","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00815","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50991","properties":[{"key":"EntryName","value":"PYR_CT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MATVLRNPLLSPSPSTSLTSSQSHPNHHLLRFPHSSKPSHSRLQSFFTPHTHTPSNLTVSCSQSQPSPRPRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASMTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKMIAKEVGNAVDDDGYVPVICGLSRCNEKDIQTAWEAVKFAKRPRVHTFIATSPIHMEYKLRKSKEQVIEIARTMVKFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGITMPGEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTVEGARAGARQLEVTINGIGERAGNASLEEVVMALKCGEKFFKGLYTGINTRHIYLTSRMVEEYTGLQLQPHKALVGANAFAHESGIHQDGMLKHKETYEIISPEVIGFERSNEAGIVLGKLSGRHALKKRLEELGYELNDDQVQNLFWRFKAVAEQKKRVTDADLRALVSDEVFQAEPVWKLGDLQVTCGTLGLSTATVKLYGVDGNTHVACSVGTGPVDSAYKAVDLIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGEKNHTSTHVLTGETVHRTFSGTGAGMDVVVSSVKAYISALNKMSSFKDTPST","length":615,"molWeight":67097,"crc64":"C5BB0301C0A9D224","md5":"1A83D0337CA0D5A02A65D5A82002A02A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Region":1,"Compositional bias":2},"uniParcId":"UPI0009E122B0"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFQ4","uniProtkbId":"A0A1V0QFQ4_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2017-06-07","entryVersion":28,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67149.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012973"}],"value":"2-isopropylmalate synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012973"}],"value":"2.3.3.13"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67149.1"}],"value":"IPMS"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00059544"}],"value":"Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Involved in Leu biosynthesis, but does not participate in the chain elongation of glucosinolates"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21524"},{"database":"ChEBI","id":"CHEBI:1178"},{"database":"ChEBI","id":"CHEBI:11851"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"}],"ecNumber":"2.3.3.13","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000064"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004689"}],"value":"Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009396"}],"value":"Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50991"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022605"}],"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023304"}],"id":"KW-0100","category":"Biological process","name":"Branched-chain amino acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022430"}],"id":"KW-0432","category":"Biological process","name":"Leucine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67149.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY618807","properties":[{"key":"ProteinId","value":"ARE67149.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFQ4","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00048","properties":[{"key":"RectionId","value":"UER00070"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"CDD","id":"cd07940","properties":[{"key":"EntryName","value":"DRE_TIM_IPMS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000010","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.238.260:FF:000003","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase 1 chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.160.270:FF:000004","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase B"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.238.260","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.160.270","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01025","properties":[{"key":"EntryName","value":"LeuA_type1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR013709","properties":[{"key":"EntryName","value":"2-isopropylmalate_synth_dimer"}]},{"database":"InterPro","id":"IPR002034","properties":[{"key":"EntryName","value":"AIPM/Hcit_synth_CS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR054691","properties":[{"key":"EntryName","value":"LeuA/HCS_post-cat"}]},{"database":"InterPro","id":"IPR036230","properties":[{"key":"EntryName","value":"LeuA_allosteric_dom_sf"}]},{"database":"InterPro","id":"IPR005671","properties":[{"key":"EntryName","value":"LeuA_bact_synth"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"NCBIfam","id":"TIGR00973","properties":[{"key":"EntryName","value":"leuA_bact"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF002086","properties":[{"key":"EntryName","value":"PRK00915.1-3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277:SF9","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22617","properties":[{"key":"EntryName","value":"HCS_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08502","properties":[{"key":"EntryName","value":"LeuA_dimer"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00917","properties":[{"key":"EntryName","value":"LeuA_dimer"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF110921","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00815","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50991","properties":[{"key":"EntryName","value":"PYR_CT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MATVLRNPLLSPSASTSVTSSQSHPNHLLLLRFPHSSKPSHSRLQSFFTPRTHTPSNLTISCSQSQSSPRPRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASMTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKMIANEVGNAVDDDGYVPVICGLSRCNEKDIQTAWEAVKYAKRPRVHTFIATSPIHMEYKLRKSKEQVIEIARTMVKFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGITMPGEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTVEGARAGARQLEVTINGIGERAGNASLEEVVMALKCGENFFKGLYTGINTRHIYLTSRMVEEYTGLQLQPHKALVGANAFAHESGIHQDGMLKHKETYEIISPDVIGFERSNEAGIVLGKLSGRHALKKRLEELGYELNDDQVQNLFWRFKAVAEQKKRVTDADLRALVSDEVFQAEPVWKLGDLQVTCGTLGLSTATVKLYGVDGNTRVACSVGTGPVDSAYKAVDLIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGDKNHTSTHALTGEPVHRTFSGTGAGMDVVVSSVKAYISALNKMSSFKDTPST","length":616,"molWeight":67116,"crc64":"F9150E1B5F6FFA2B","md5":"3D252B76EFF473D432DFB193ED68EEE5"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Region":2,"Compositional bias":3},"uniParcId":"UPI0009E0F103"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFL5","uniProtkbId":"A0A1V0QFL5_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2025-10-08","lastSequenceUpdateDate":"2017-06-07","entryVersion":19,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67141.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67141.1"}],"value":"FEN1"}}],"flag":"Fragment"},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"value":"Belongs to the alpha-IPM synthase/homocitrate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50991"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67141.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":153,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67141.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67141.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY412794","properties":[{"key":"ProteinId","value":"ARE67141.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFL5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR002034","properties":[{"key":"EntryName","value":"AIPM/Hcit_synth_CS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"PANTHER","id":"PTHR10277:SF74","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00815","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50991","properties":[{"key":"EntryName","value":"PYR_CT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"PHYIPNHIPDPSYVRILDTTLRDGEQSPGAAMTPHQKLDIARQLWKLGVDVIEAGFPCSSENDFNAVKMISEEVGNHVSVDGYVPVIIGVARCNEKDIQTAWDAIQHAKRPRISTFIATSPIHMEHKLKKTKEEVLEIAGRMVRFARSLGCDD","length":153,"molWeight":17099,"crc64":"529AE83BB224114F","md5":"8D3435A163034652A103007D5CAABE39"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI0009E11DC8"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFL6","uniProtkbId":"A0A1V0QFL6_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2025-10-08","lastSequenceUpdateDate":"2017-06-07","entryVersion":24,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67142.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67142.1"}],"value":"FEN1"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"value":"Belongs to the alpha-IPM synthase/homocitrate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50991"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022605"}],"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023304"}],"id":"KW-0100","category":"Biological process","name":"Branched-chain amino acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67142.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY412795","properties":[{"key":"ProteinId","value":"ARE67142.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFL6","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010177","properties":[{"key":"GoTerm","value":"F:methylthioalkylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0019761","properties":[{"key":"GoTerm","value":"P:glucosinolate biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd07940","properties":[{"key":"EntryName","value":"DRE_TIM_IPMS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.238.260:FF:000001","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000010","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.238.260","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.160.740","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR013709","properties":[{"key":"EntryName","value":"2-isopropylmalate_synth_dimer"}]},{"database":"InterPro","id":"IPR002034","properties":[{"key":"EntryName","value":"AIPM/Hcit_synth_CS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR054691","properties":[{"key":"EntryName","value":"LeuA/HCS_post-cat"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"NCBIfam","id":"NF002086","properties":[{"key":"EntryName","value":"PRK00915.1-3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277:SF74","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22617","properties":[{"key":"EntryName","value":"HCS_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08502","properties":[{"key":"EntryName","value":"LeuA_dimer"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00815","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00816","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50991","properties":[{"key":"EntryName","value":"PYR_CT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTRLHYIPNHIPDPSYVRILDTTLRDGEQSPGAAMTPRQKLEIARQLSKLGVDVIEAGFPCSSENEFIAVKMIAEEVGNYVGDDGYIPVINGVARCNEKDIATTWEAVRNAKRPKVTTFIATSGIHMEHKLKKTKEEVLEIACRMVRFARSLGCDDVQFVPEDAARSEREFLYQIIGEAIKAGAASVGIPDTVGIAMPWEYGKLIADIKANTPGIENVVISTHCHNDFGLAVANTIEGARAGARQLEVTINGIGERAGNASFEEVVMALKCRGHDVFGGLYTGINTKHIFKTSKMVEEYTGMHLQPHKALVGANAFVHESGIHQDGWLKNKATYEIIPPESIGLERSSATGIVLGKLSGRHALKKRLEELGYQLQDDEIANLFWHFKAVADKKKRVADADLIALVSNEVTKAESIWKLGDLQVICGTLGLSTATVKLIDTHGNEHVACSVGSGSVDSAFKAVNLIVKVSKFN","length":472,"molWeight":51441,"crc64":"9A58A7F64340D94A","md5":"082FD88DB13C5F35C980CCD7C10C2190"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Domain":1},"uniParcId":"UPI0009E134E1"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFL7","uniProtkbId":"A0A1V0QFL7_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2025-10-08","lastSequenceUpdateDate":"2017-06-07","entryVersion":19,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67140.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67140.1"}],"value":"FEN1"}}],"flag":"Fragment"},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"value":"Belongs to the alpha-IPM synthase/homocitrate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50991"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67140.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":153,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67140.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67140.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY412793","properties":[{"key":"ProteinId","value":"ARE67140.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFL7","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR002034","properties":[{"key":"EntryName","value":"AIPM/Hcit_synth_CS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"PANTHER","id":"PTHR10277:SF74","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00815","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50991","properties":[{"key":"EntryName","value":"PYR_CT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"PHYIPNHIPDPSYVRILDTTLRDGEQSPGAAMTPHQKLDIARQLWKLGVDVIEAGFPCSSENDFNAVKMISEEVGNHVGVDGYVPVIIGVARCNEKDIQIAWDAIKHAKRPRISTFIATSPIHMEHKLKKTKEEVLEIACRMVRFARSLGCDD","length":153,"molWeight":17127,"crc64":"395C850EF624D6CD","md5":"B0E018F16F5F2A1408C43841E52D4A80"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI0009E122FC"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFM1","uniProtkbId":"A0A1V0QFM1_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2025-10-08","lastSequenceUpdateDate":"2017-06-07","entryVersion":24,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67143.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67143.1"}],"value":"FEN1"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"value":"Belongs to the alpha-IPM synthase/homocitrate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50991"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022605"}],"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023304"}],"id":"KW-0100","category":"Biological process","name":"Branched-chain amino acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67143.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY412796","properties":[{"key":"ProteinId","value":"ARE67143.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFM1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010177","properties":[{"key":"GoTerm","value":"F:methylthioalkylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0019761","properties":[{"key":"GoTerm","value":"P:glucosinolate biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd07940","properties":[{"key":"EntryName","value":"DRE_TIM_IPMS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.238.260:FF:000001","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000010","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.238.260","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.160.740","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR013709","properties":[{"key":"EntryName","value":"2-isopropylmalate_synth_dimer"}]},{"database":"InterPro","id":"IPR002034","properties":[{"key":"EntryName","value":"AIPM/Hcit_synth_CS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR054691","properties":[{"key":"EntryName","value":"LeuA/HCS_post-cat"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"NCBIfam","id":"NF002086","properties":[{"key":"EntryName","value":"PRK00915.1-3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277:SF74","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22617","properties":[{"key":"EntryName","value":"HCS_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08502","properties":[{"key":"EntryName","value":"LeuA_dimer"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00815","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00816","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50991","properties":[{"key":"EntryName","value":"PYR_CT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKRPQYIPNHIPDPSYVRILDTTLRDGEQSPGAAMTPHQKLDIARQLWKLGVDVIEAGFPCSSENDFNAVKMISEEVGNHVSVDGYVPVIIGVARCNEKDIQTAWDAIKHAKRPRISTFIATSPIHMEHKLKKTKEEVLEIAGRMVRFARSLGCDDVQFVPEDAARSERGFLYEIIGEVIKAGATTVDIPDTVGITMPWEFGKLIADIKANTHGIQNVVISTHCHNDLGLAVANTIEGARAGARQLEVTINGIGERAGNASLEEVVMALKCRGHEAFDGLYTGINTKHIFKTSKMVEEYTGMHVQPHKALVGANAFVHESGIHQDGWLKHKATYEIIPPESIGLERLSGSGIVLGKLSGRQALRKRLQELGYQLKDDEVASVFLNFKAVADKKKRVTDADLIALVSNEVTKAESIWKLGDLQVTCGTLDLSTATVKLISIDGNEHVACSVGSGSVDSAFKAVNLIVKVLKFNYAFCCHYFLFFILRDKIYFSSNNFE","length":497,"molWeight":54726,"crc64":"2E4C26D45C13A1CE","md5":"0547488D800627AE784EC9EF1084AD31"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Domain":1},"uniParcId":"UPI0009E1179D"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFM4","uniProtkbId":"A0A1V0QFM4_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"2017-06-07","entryVersion":17,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67145.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67145.1"}],"value":"FEN1"}}],"flag":"Fragment"},"features":[{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50991"}]},{"type":"Non-terminal residue","location":{"start":{"value":372,"modifier":"EXACT"},"end":{"value":372,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67145.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67145.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY412798","properties":[{"key":"ProteinId","value":"ARE67145.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFM4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd07940","properties":[{"key":"EntryName","value":"DRE_TIM_IPMS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000010","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.238.260","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR002034","properties":[{"key":"EntryName","value":"AIPM/Hcit_synth_CS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR054691","properties":[{"key":"EntryName","value":"LeuA/HCS_post-cat"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"PANTHER","id":"PTHR10277:SF74","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22617","properties":[{"key":"EntryName","value":"HCS_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00816","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50991","properties":[{"key":"EntryName","value":"PYR_CT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTRPHYIPNHISDPSYVRILDTTLRDGEPSPGAAMTPRQKLEIARQLSKLGVDVIEAGFPCSSQNEFIAVKMIAEEVGNYVGDDGYIPVINGVARCNEKDIATTWEAVRNAKRPKVTTFIATSGIHMEHKLKKTKEEVLEIACRMVRFARSLGCDDVQFVPEDAVRSEREFLYQIIGEAIKAGAASVGIPDTVGIAMPWEYGKLIADIKANTPGIQNVVISTHCHNDFGLAVANTIEGARAGARQLEVTINGIGERAGNASFEEVVMALKCRGHDVFGGLYTGINTKHIFKTSKMVEEYTGMHIQPHKALVGANAFVHESGIHQHGWLKNKATYEIIAPESIGLERSSAAGIVLGKLSGRHALKKRLEELGY","length":372,"molWeight":40641,"crc64":"FC7079A25CE1EB37","md5":"4420274E8B0EA35E19B0E5AFDB29CFB7"},"extraAttributes":{"countByFeatureType":{"Domain":1,"Non-terminal residue":1},"uniParcId":"UPI0009E0FC8E"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFN2","uniProtkbId":"A0A1V0QFN2_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2025-10-08","lastSequenceUpdateDate":"2017-06-07","entryVersion":16,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67138.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67138.1"}],"value":"FEN1"}}],"flag":"Fragment"},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"value":"Belongs to the alpha-IPM synthase/homocitrate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50991"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67138.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67138.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67138.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY412791","properties":[{"key":"ProteinId","value":"ARE67138.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFN2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR002034","properties":[{"key":"EntryName","value":"AIPM/Hcit_synth_CS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"PANTHER","id":"PTHR10277:SF74","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00815","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50991","properties":[{"key":"EntryName","value":"PYR_CT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"PHYIPNHIPDPSYVRILDTTLRDGEQSPGAAMTPRQKLEIARQLSKLGVDVIEAGFPCSSENEFIAVKMIAEEVGNYVGDDGYIPVINGVARCNEKDIATTWEAVRNAKRPKVTTFIATSGIHMEHKLKKTKEEVLEIACRMVRFARSLGCDDVQFVPEDAA","length":162,"molWeight":17970,"crc64":"FF9DBDC397C8682D","md5":"E1D94C055FC0C8E2D1D48EFB9646F51A"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI0009E11C08"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFP5","uniProtkbId":"A0A1V0QFP5_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"2017-06-07","entryVersion":11,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67139.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"4: Predicted","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67139.1"}],"value":"FEN1"}}],"flag":"Fragment"},"features":[{"type":"Domain","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00682"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67139.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67139.1"}]}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67139.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY412792","properties":[{"key":"ProteinId","value":"ARE67139.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFP5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"PANTHER","id":"PTHR10277:SF74","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"PHYIPNHISDPSYVRILDTTLRDGEPSPGAAMTPRQKLEIARQLSKLGVDVIEAGFPCSSQNEFIAVKMIAEEVGNYVGDDGYIPVINGVARCNEKDIATTWEAVRNAKRPKVTTFIATSGIHMEHKLKKTKEEVLEIACRMVRFARSLGCDDVQFVPEDAA","length":162,"molWeight":17927,"crc64":"C368C5D873E768B7","md5":"D1FD249BF26CC9C7E721C46FE79A34B5"},"extraAttributes":{"countByFeatureType":{"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI0009E0F3B2"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1V0QFQ9","uniProtkbId":"A0A1V0QFQ9_9FABA","entryAudit":{"firstPublicDate":"2017-06-07","lastAnnotationUpdateDate":"2025-06-18","lastSequenceUpdateDate":"2017-06-07","entryVersion":17,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Aeschynomene afraspera","taxonId":552031,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67146.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","fabids","Fabales","Fabaceae","Papilionoideae","50 kb inversion clade","dalbergioids sensu lato","Dalbergieae","Dalbergia clade","Aeschynomene"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67146.1"}],"value":"FEN1"}}],"flag":"Fragment"},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"value":"Belongs to the alpha-IPM synthase/homocitrate synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Pyruvate carboxyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50991"}]},{"type":"Non-terminal residue","location":{"start":{"value":304,"modifier":"EXACT"},"end":{"value":304,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67146.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003523"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"28348373","citationType":"journal article","authors":["Nouwen N.","Arrighi J.F.","Cartieaux F.","Chaintreuil C.","Gully D.","Klopp C.","Giraud E."],"citationCrossReferences":[{"database":"PubMed","id":"28348373"}],"title":"The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in Aeschynomene/photosynthetic Bradyrhizobium symbiosis.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"448","lastPage":"448","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ARE67146.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KY412799","properties":[{"key":"ProteinId","value":"ARE67146.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"A0A1V0QFQ9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003852","properties":[{"key":"GoTerm","value":"F:2-isopropylmalate synthase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009098","properties":[{"key":"GoTerm","value":"P:L-leucine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd07940","properties":[{"key":"EntryName","value":"DRE_TIM_IPMS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000010","properties":[{"key":"EntryName","value":"2-isopropylmalate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050073","properties":[{"key":"EntryName","value":"2-IPM_HCS-like"}]},{"database":"InterPro","id":"IPR002034","properties":[{"key":"EntryName","value":"AIPM/Hcit_synth_CS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR000891","properties":[{"key":"EntryName","value":"PYR_CT"}]},{"database":"PANTHER","id":"PTHR10277:SF74","properties":[{"key":"EntryName","value":"2-ISOPROPYLMALATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10277","properties":[{"key":"EntryName","value":"HOMOCITRATE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00682","properties":[{"key":"EntryName","value":"HMGL-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00815","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00816","properties":[{"key":"EntryName","value":"AIPM_HOMOCIT_SYNTH_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50991","properties":[{"key":"EntryName","value":"PYR_CT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKRPQYIPNHIPDPSYVRILDTTLRDGEQSPGAAMTPHQKLDIARQLWKLGVDVIEAGFPCSSENDFNAVKMISEEVGNHVGVDGYVPVIIGVARCNEKDIQIAWDAIKHAKRPRISTFIATSPIHMEHKLKKTKEEVLEIACRMVRFARSLGCDDIQFVPEDAARSEREFLYEIIGEVIKAGATTVDIPDTVGITMPWEFGKLIADIKANTHGIENVVISTHCHNDLGLAVANTIEGARAGARQLEVTINGIGERAGNASLEEVVMALKCRGHEAFDGLYTGINTKHIFKTSKMVEEYTGMHI","length":304,"molWeight":33597,"crc64":"D9ADF28F81D5A082","md5":"55DDF7F37E2F032495DC5DC8FC987EE8"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Non-terminal residue":1},"uniParcId":"UPI0009E112FA"}}]}