{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8A8","uniProtkbId":"B3Y8A8_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65640.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65640.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65640.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65640.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65640.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65640.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65640.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65640.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65640.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449145","properties":[{"key":"ProteinId","value":"BAG65640.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8A8","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGLNQICMRKLLAYSSINHVSWMIAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24212,"crc64":"E088F0975B9FA8A8","md5":"0B550ED4A31D7BFB980E77FD57B426E7"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A0F"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8A9","uniProtkbId":"B3Y8A9_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65641.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65641.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65641.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65641.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65641.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65641.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65641.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65641.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65641.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449146","properties":[{"key":"ProteinId","value":"BAG65641.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8A9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGLNQICMRKLLAYSSINHGSGMIAAMLNSMSIGLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":23912,"crc64":"40D8ECFAF0F3DFB2","md5":"DE9B54071299B32B505CEE851B59313C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A10"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8B0","uniProtkbId":"B3Y8B0_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65642.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65642.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65642.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65642.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65642.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65642.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65642.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65642.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65642.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449147","properties":[{"key":"ProteinId","value":"BAG65642.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8B0","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSPMISGILGLNQICMRKLLAYSPINHVSWMIAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24232,"crc64":"E1E40D04589C6467","md5":"896731B810AED4316520C9B2AF6D98F7"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A11"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8B1","uniProtkbId":"B3Y8B1_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65643.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65643.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65643.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65643.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65643.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65643.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65643.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65643.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65643.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449148","properties":[{"key":"ProteinId","value":"BAG65643.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8B1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSLIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVLSGLDWSNSFIMLTWQKIAPMILLSYLINSHILLFSIIIILSSMISGILGLNQICMRKLLAYSSINHISWMIAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24273,"crc64":"229868BBA24AAC4C","md5":"E35672BBA1CCAC51EC9A27E64DC28B73"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A12"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8B2","uniProtkbId":"B3Y8B2_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65644.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65644.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65644.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65644.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65644.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65644.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65644.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65644.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65644.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449149","properties":[{"key":"ProteinId","value":"BAG65644.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8B2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGLNQICMRKLLAYSSINHVSWMIAAMLNSMSIGLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24083,"crc64":"01D8F0975B9FB219","md5":"D758655C2D348B15E02D9A208A82B811"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A13"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8B3","uniProtkbId":"B3Y8B3_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65645.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65645.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65645.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65645.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65645.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65645.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65645.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65645.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65645.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449150","properties":[{"key":"ProteinId","value":"BAG65645.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8B3","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHIWLFSITIIWSSMISGILGLNQICMRKLLAYSSINHVSWMIAAMLNSMSIWLYYFLNYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24359,"crc64":"1FD2053C5024A8A4","md5":"353D4610114363ECCB1614BBD33CDC1B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A14"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8B4","uniProtkbId":"B3Y8B4_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":40,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65646.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65646.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65646.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65646.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65646.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65646.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65646.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65646.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65646.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449151","properties":[{"key":"ProteinId","value":"BAG65646.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB449153","properties":[{"key":"ProteinId","value":"BAG65648.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8B4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVLSGLDWSNSFIMLTWQKIAPMILLSYLINSHILLFSIIIILSSMISGILGLNQICMRKLLAYSSINHISWMIAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24273,"crc64":"56CB89BFF06CDF69","md5":"E67E99C1648865247703456D2A3A58E0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A15"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8B5","uniProtkbId":"B3Y8B5_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65647.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65647.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65647.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65647.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65647.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65647.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65647.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65647.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65647.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449152","properties":[{"key":"ProteinId","value":"BAG65647.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8B5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGLNQICMRKLLAYSSINHVSWMIAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24246,"crc64":"84C49C77BB91A64C","md5":"6ED36722054A19528D2C7F9B970AEDB6"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A16"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8B7","uniProtkbId":"B3Y8B7_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65649.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65649.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":67,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65649.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65649.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65649.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65649.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65649.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65649.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65649.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449154","properties":[{"key":"ProteinId","value":"BAG65649.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8B7","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVLSGLDWSNSFIMLTWQKIAPMILLSYLINSHILLFSINNILSSISSGILGLNQICMRKLLAYSSINHISWMMAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24249,"crc64":"A6182447DEDF8282","md5":"05B2959DBFED021B51B201F399361CF8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":4,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A17"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8B8","uniProtkbId":"B3Y8B8_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65650.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65650.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65650.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65650.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65650.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65650.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65650.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65650.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65650.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449155","properties":[{"key":"ProteinId","value":"BAG65650.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8B8","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGLNQICMRKLLAYSSINHVSWMMAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24230,"crc64":"E7939D269B9FA8A8","md5":"1944852DC1145327FAE529386B4079F1"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A18"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8B9","uniProtkbId":"B3Y8B9_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65651.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65651.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65651.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65651.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65651.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65651.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65651.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65651.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65651.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449156","properties":[{"key":"ProteinId","value":"BAG65651.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8B9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGLNQICMRKLLAYSSINHVSWMMAAMLKSMSIGLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24115,"crc64":"06C39D228FCEF319","md5":"0F804B1F3459C7DBC86BE126B7F9052A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A19"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C0","uniProtkbId":"B3Y8C0_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-09-23","entryVersion":37,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65652.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65652.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65652.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65652.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65652.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65652.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65652.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65652.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65652.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449157","properties":[{"key":"ProteinId","value":"BAG65652.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C0","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGTKPICMRKLLAYSSINHGSWMIAAMLNSMSIGLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24012,"crc64":"EEEF8896F0E53884","md5":"4974A4130DAEFF123E4ED227107D8F43"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":3,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A1A"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C1","uniProtkbId":"B3Y8C1_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-09-23","entryVersion":37,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65653.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65653.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65653.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65653.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65653.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65653.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65653.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65653.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65653.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449158","properties":[{"key":"ProteinId","value":"BAG65653.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGTKPICMRKLLAYSSINHGSWMIAAMLNSMSIGLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24046,"crc64":"8AA3E47610EB3660","md5":"FDD2E4220D2F846CDB3762227A2BF0EE"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":3,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A1B"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C2","uniProtkbId":"B3Y8C2_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-09-23","entryVersion":37,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65654.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65654.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65654.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65654.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65654.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65654.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65654.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65654.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65654.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449159","properties":[{"key":"ProteinId","value":"BAG65654.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGTKPICLRKLLAYSSINHGSWMIAAMLNSMSIGLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":23994,"crc64":"EF5E389741553884","md5":"4A22B1A4EC3F02AF08F98FACBECAE28D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":3,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A1C"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C3","uniProtkbId":"B3Y8C3_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65655.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65655.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65655.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65655.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65655.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65655.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65655.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65655.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65655.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449160","properties":[{"key":"ProteinId","value":"BAG65655.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C3","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGIPGTNQICMRKLLAYSSIHHGSWMIAAMLNSMSIGLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24036,"crc64":"89D495E02A224DB4","md5":"68633D3DCE45490DFDD9A44F77D82830"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":4,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A1D"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C4","uniProtkbId":"B3Y8C4_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65656.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65656.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65656.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65656.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65656.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65656.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65656.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65656.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65656.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449161","properties":[{"key":"ProteinId","value":"BAG65656.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSLNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVISGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSITIILSSMISGILGLNQICMRKLLAYSSINHVSWMIAAMLNSMSIGLNYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24034,"crc64":"01D8F097457F5C09","md5":"1E33D5A4ED17FE2EFEEC16B0E38B1C09"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A1E"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C5","uniProtkbId":"B3Y8C5_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65657.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65657.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65657.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65657.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65657.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65657.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65657.1"}],"value":"Clade A","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65657.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65657.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449162","properties":[{"key":"ProteinId","value":"BAG65657.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKTPKNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVLSGLDWSNSFIMLTWQKIAPMILLSYLINSHILLFSIIIILSSMISGILGLNQICMRKLLAYSSINHISWMMAAMLNSKSIGLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24159,"crc64":"B08703904E6CC5D8","md5":"0277343DBF9351902BC84A3A9B1111C3"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A1F"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C6","uniProtkbId":"B3Y8C6_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65658.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65658.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65658.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65658.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65658.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65658.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65658.1"}],"value":"Clade B","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65658.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65658.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449163","properties":[{"key":"ProteinId","value":"BAG65658.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C6","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKNPNNKYPSEAAIKYFMAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVLSGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSVIIILSSMISGILGLNQICMRKLLAYSSINHVSWMIAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24261,"crc64":"3768AC0708AA593D","md5":"342C7DFD4E473B9D30A0C4D2D586B1B2"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A21"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C7","uniProtkbId":"B3Y8C7_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":42,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65659.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65659.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65659.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65659.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65659.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65659.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65659.1"}],"value":"Clade B","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65659.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65659.1"}]},{"referenceNumber":2,"citation":{"id":"23537238","citationType":"journal article","authors":["Tada Y.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"23537238"},{"database":"DOI","id":"10.2108/zsj.30.282"}],"title":"Geographic Distributions of Sexual and Parthenogenetic Forms of the Weevil Scepticus insularis (Coleoptera: Curculionidae) in Japan.","publicationDate":"2013","journal":"Zool. Sci.","firstPage":"282","lastPage":"288","volume":"30"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAK57370.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAK57370.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449164","properties":[{"key":"ProteinId","value":"BAG65659.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB449800","properties":[{"key":"ProteinId","value":"BAG66077.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB449804","properties":[{"key":"ProteinId","value":"BAG66081.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB449806","properties":[{"key":"ProteinId","value":"BAG66083.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB449808","properties":[{"key":"ProteinId","value":"BAG66085.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB661323","properties":[{"key":"ProteinId","value":"BAK57370.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB661324","properties":[{"key":"ProteinId","value":"BAK57371.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB661325","properties":[{"key":"ProteinId","value":"BAK57372.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB661326","properties":[{"key":"ProteinId","value":"BAK57373.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C7","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKNPNNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVLSGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSVIIILSSMISGILGLNQICMRKLLAYSSINHVSWMIAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24243,"crc64":"BC0602AEB96D4252","md5":"386BF68190EDDD6B53AE594389BD5CAD"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A22"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C8","uniProtkbId":"B3Y8C8_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":40,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65660.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65660.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65660.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65660.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65660.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65660.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65660.1"}],"value":"Clade B","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65660.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65660.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449165","properties":[{"key":"ProteinId","value":"BAG65660.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB449802","properties":[{"key":"ProteinId","value":"BAG66079.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB449805","properties":[{"key":"ProteinId","value":"BAG66082.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB449807","properties":[{"key":"ProteinId","value":"BAG66084.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C8","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKNPNNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTISSTALLLKMGAAPFHSWFPEVLSGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSVIIILSSMISGILGLNQICMRKLLAYSSINHVSWMIAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24229,"crc64":"BC0607AEEAB61489","md5":"D560E67D7624A249B7D974B121CC9689"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A23"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8C9","uniProtkbId":"B3Y8C9_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65661.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65661.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65661.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65661.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65661.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65661.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65661.1"}],"value":"Clade B","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65661.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65661.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449166","properties":[{"key":"ProteinId","value":"BAG65661.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8C9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKNPNNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTISSTALLLKMGAAPFHSWFPEVLSGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSVIIILSSMISGILGLNQICMRKLLAYSSINHVSWMIAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKS","length":211,"molWeight":24188,"crc64":"AA8607AEEAB61489","md5":"A99981D9FD12969630FF48431624B435"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A24"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8D0","uniProtkbId":"B3Y8D0_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65662.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65662.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65662.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65662.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65662.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65662.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65662.1"}],"value":"Clade B","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65662.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65662.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449167","properties":[{"key":"ProteinId","value":"BAG65662.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8D0","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKNPNNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTISSTALLLKMGAAPFHSWFPEVLSGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSGIIILSSMISGILGLNQICMRELLAYSSINHVSWMMAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24206,"crc64":"AB1442235AAA76F7","md5":"1D10C212BC6CC2EB969BB4487815FAD9"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A25"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8D1","uniProtkbId":"B3Y8D1_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65663.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65663.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65663.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65663.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65663.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65663.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65663.1"}],"value":"Clade B","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65663.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG65663.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449168","properties":[{"key":"ProteinId","value":"BAG65663.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8D1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKNPNNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVLSGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSGMIILSSMISGILGLNQICMRELLGYSSINHVSWMMAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24224,"crc64":"1F14404FA2B64C87","md5":"B3B66E440DD7C1985185B53C37AA3197"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017A8A26"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8P1","uniProtkbId":"B3Y8P1_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-09-23","entryVersion":37,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66075.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66075.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":172,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66075.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66075.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66075.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66075.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66075.1"}],"value":"Clade B","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66075.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66075.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449798","properties":[{"key":"ProteinId","value":"BAG66075.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8P1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKNPNNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTITSTALLLKMGAAPFHSWFPEVLSGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSGIIILSSMISGILGLNQICMRKLLAYSSINPVSWKMAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24176,"crc64":"4300F9D86043754F","md5":"68F1DE566B8FCA0E65ADDA1860A4B66B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":3,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017B5ADC"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B3Y8P2","uniProtkbId":"B3Y8P2_SCEIN","entryAudit":{"firstPublicDate":"2008-09-23","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-09-23","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Scepticus insularis","commonName":"Weevil","taxonId":549020,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66076.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Neuropteroidea","Coleoptera","Polyphaga","Cucujiformia","Curculionidae","Entiminae","Entimini","Scepticus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"}],"value":"7.1.1.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031028"}],"value":"NADH dehydrogenase subunit 2"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66076.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66076.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66076.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66076.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66076.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"21303200","citationType":"journal article","authors":["Tada Y.","Kobayashi N.","Katakura H."],"citationCrossReferences":[{"database":"PubMed","id":"21303200"},{"database":"DOI","id":"10.2108/zsj.28.90"}],"title":"Phylogenetic relationships among bisexual and unisexual lineages of the weevil Scepticus insularis (Coleoptera: Curculionidae) on Hokkaido, Northern Japan.","publicationDate":"2011","journal":"Zool. Sci.","firstPage":"90","lastPage":"97","volume":"28"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66076.1"}],"value":"Clade B","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66076.1"}],"value":"Muscle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAG66076.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB449799","properties":[{"key":"ProteinId","value":"BAG66076.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B3Y8P2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"WLSSWIGLEINLLSIIPILKNPNNKYPSEAAIKYFIAQVMASSLLLFSIVSFNCLKESSFQYLESYETTISSTALLLKMGAAPFHSWFPEVLSGLNWSNSFIMLTWQKIAPMILLSYLINSHILLFSGIIILSSMISGILGLNQICMRKLLAYSSINPGSWMMAAMLNSMSIWLYYFLIYSFINLNIIILFKKYNIFYLNQLTNIFNSSKK","length":211,"molWeight":24123,"crc64":"02046A0347134E3F","md5":"B064CB9D193E9F519D57310103DF0832"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI00017B5ADD"}}]}