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In addition, exhibits a broad substrate specificity and can catalyze the hydrolysis of various phosphomonoesters, diesters, triesters and phosphothiolates (PubMed:14711669, PubMed:168197, PubMed:17630782). Preferentially hydrolyzes the phosphate diesters over the phosphonate monoesters (PubMed:17630782). Can hydrolyze the model substrates p-nitrophenyl phosphate (pNPP), bis-(p-nitrophenyl phosphate) (bis(pNPP)) and ethyl p-nitrophenyl phosphate (EtpNPP) (PubMed:14711669, PubMed:168197, PubMed:17306828, PubMed:17630782, PubMed:18678932, PubMed:18831553). Also exhibits activity towards some organophosphate pesticides and is capable of hydrolyzing a close analog of EA 2192, the most toxic and persistent degradation product of the nerve agent VX (PubMed:14711669, PubMed:17630782)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:12969"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30879"},{"database":"ChEBI","id":"CHEBI:57597"},{"database":"ChEBI","id":"CHEBI:83408"}],"ecNumber":"3.1.4.46","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14711669"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"sn-glycero-3-phosphoethanolamine + H2O = ethanolamine + sn-glycerol 3-phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29319"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57597"},{"database":"ChEBI","id":"CHEBI:57603"},{"database":"ChEBI","id":"CHEBI:143890"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14711669"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Fe(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18678932"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29033"}},{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17306828"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17630782"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18678932"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}},{"name":"Co(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17306828"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18831553"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:48828"}},{"name":"Cd(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18535849"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:48775"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17630782"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17306828"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17630782"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18535849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18678932"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18831553"}],"value":"Binds 2 Fe(2+) ions per subunit (PubMed:18678932). Active in the presence of various divalent cations such as Fe(2+), Zn(2+), Cd(2+), Co(2+) and Mn(2+) (PubMed:17306828, PubMed:17630782, PubMed:18535849, PubMed:18678932, PubMed:18831553). Fe(2+) is probably the native metal ion (PubMed:18678932)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17306828"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18678932"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18831553"}],"value":"The active site contains a binuclear metal center, with a fully occupied alpha-metal ion site, and partially occupied beta-metal ion site (PubMed:17306828, PubMed:18678932, PubMed:18831553). The presence of substrate promotes the formation of a catalytically competent binuclear center by significantly enhancing the binding affinity of one of the metal ions in the active site (PubMed:18831553)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":7.0,"unit":"mM","substrate":"bis(pNPP) (in the presence of Fe(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18678932"}]},{"constant":3.5,"unit":"mM","substrate":"bis(pNPP) (in the presence of Zn(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18678932"}]},{"constant":2.9,"unit":"mM","substrate":"bis(pNPP) (in the presence of Zn(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17306828"}]},{"constant":1.4,"unit":"mM","substrate":"bis(pNPP) (in the presence of Co(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17306828"}]},{"constant":0.12,"unit":"mM","substrate":"bis(pNPP) (in the presence of Co(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18831553"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17306828"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18535849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18678932"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18831553"}],"value":"kcat is 2.78 sec(-1) with bis(pNPP) as substrate (in the presence of Fe(2+)) (PubMed:18678932). kcat is 0.176 sec(-1) with bis(pNPP) as substrate (in the presence of Zn(2+)) (PubMed:18678932). kcat is 0.107 sec(-1) with bis(pNPP) as substrate (in the presence of Zn(2+)) (PubMed:17306828). kcat is 1.62 sec(-1) with bis(pNPP) as substrate (in the presence of Co(2+)) (PubMed:17306828). kcat is 1.89 sec(-1) with bis(pNPP) as substrate (in the presence of Co(2+)) (PubMed:18831553). kcat is 15 sec(-1) with bis(pNPP) as substrate (in the presence of Cd(2+)) (PubMed:18535849)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"168197"}],"value":"Optimum pH is 5.0 for mono- and diesters hydrolysis."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"168197"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16820687"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17306828"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18831553"}],"value":"Homohexamer (PubMed:168197, PubMed:17306828, PubMed:18831553). Trimer of dimers (PubMed:17306828). Tends to form a dimer under crystallization conditions (PubMed:16820687)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14711669"}],"value":"Induced under phosphate-limiting conditions"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14711669"}],"value":"Deletion mutant is unable to grow by using dimethyl phosphate (DMP) or the phosphotriesters as phosphorus sources"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17630782"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18535849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24239906"}],"value":"Is of interest for its potential application as a bioremediator in the destruction of organophosphate pesticides and in the detoxification of nerve agents (PubMed:17630782, PubMed:18535849). The Ser127Ala mutant, which possesses improved metal binding abilities, has been immobilized on magnetite nanoparticles and shown to be active for multiple cycles, and could be stored over a prolonged time in the immobilized form without loss of activity. This system has the potential to be of potential use in the bioremediation of organophosphate contaminated waterways (PubMed:24239906)"}],"commentType":"BIOTECHNOLOGY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19923005"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20163105"}],"value":"Diester bonds are cleaved in two separate (non-processive) reactions, indicating that GpdQ may regenerate its active site after the first hydrolysis (PubMed:19923005). The metal ion-bridging hydroxide molecule activates a terminally bound hydroxide nucleophile (PubMed:20163105)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the cyclic nucleotide phosphodiesterase class-III family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"Glycerophosphodiester phosphodiesterase GpdQ","featureId":"PRO_0000453792"},{"type":"Binding 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