{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q38710","secondaryAccessions":["Q94FW1"],"uniProtkbId":"TPSDV_ABIGR","entryAudit":{"firstPublicDate":"2005-04-12","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"1996-11-01","entryVersion":124,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Bifunctional abietadiene synthase, chloroplastic"}},"alternativeNames":[{"fullName":{"value":"(-)-abieta-7(8),13(14)-diene synthase"}},{"fullName":{"value":"Abietadiene cyclase"},"shortNames":[{"value":"AgAS"}]},{"fullName":{"value":"Agggabi"}}],"includes":[{"recommendedName":{"fullName":{"value":"Abietadiene synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10814381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"}],"value":"4.2.3.18"}]},"alternativeNames":[{"fullName":{"value":"Neoabietadiene synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10814381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"}],"value":"4.2.3.132"}]}]},{"recommendedName":{"fullName":{"value":"Copalyl diphosphate synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10814381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"}],"value":"5.5.1.12"}]}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"AS"},"synonyms":[{"value":"ac22"},{"value":"ag22"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10814381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12614165"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18052062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20430888"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to abietadiene. The copalyl diphosphate (CPP) intermediate diffuses freely between the 2 active sites in the enzyme. Changes in reaction pH, but not salt concentration, influence the relative proportion of the major products of the enzyme, abitadiene, levopimaradiene and neoabitadiene"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E,10E)-geranylgeranyl diphosphate = (+)-copalyl diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24316"},{"database":"ChEBI","id":"CHEBI:58635"},{"database":"ChEBI","id":"CHEBI:58756"}],"ecNumber":"5.5.1.12","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10814381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(+)-copalyl diphosphate = abieta-7,13-diene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13873"},{"database":"ChEBI","id":"CHEBI:30232"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58635"}],"ecNumber":"4.2.3.18","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10814381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(+)-copalyl diphosphate = neoabietadiene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:33987"},{"database":"ChEBI","id":"CHEBI:29651"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58635"}],"ecNumber":"4.2.3.132","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10814381"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q40577"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q40577"}],"value":"Binds 3 Mg(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"}],"value":"Not inhibited by 13-cyclopropylidene or 16-methylidenegeranylgeranyl diphosphate. The copalyl diphosphate synthase activity is not susceptible to magnesium-dependent inhibition"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":3.0,"unit":"uM","substrate":"GGPP (in presence of magnesium)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}]},{"constant":20.0,"unit":"uM","substrate":"GGPP (in absence of magnesium)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}]},{"constant":0.4,"unit":"uM","substrate":"CPP (in presence of magnesium)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}]},{"constant":10.0,"unit":"uM","substrate":"CPP (in absence of magnesium)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}]}]},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11112547"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"value":"Optimum pH is 7.2 with GGPP as substrate and 8.7 with CPP as substrate."}]}},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"value":"Monomer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8798524"}],"value":"By wounding"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity in the class II active site relevant for the cyclization of GGPP. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity in the class I active site, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"The N-terminus is blocked"}],"commentType":"PTM"},{"texts":[{"value":"The abietadiene synthase activity exhibits an absolute dependence on a divalent metal ion cofactor while the copalyl diphosphate synthase activity is not completely dependent"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":868,"modifier":"EXACT"}},"description":"Bifunctional abietadiene synthase, chloroplastic","featureId":"PRO_0000033634"},{"type":"Motif","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":405,"modifier":"EXACT"}},"description":"DXDD motif","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Motif","location":{"start":{"value":621,"modifier":"EXACT"},"end":{"value":625,"modifier":"EXACT"}},"description":"DDXXD motif","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Binding site","location":{"start":{"value":269,"modifier":"EXACT"},"end":{"value":269,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q38802"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":402,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"C7BKP9"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"4"}},{"type":"Binding site","location":{"start":{"value":404,"modifier":"EXACT"},"end":{"value":404,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"C7BKP9"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"4"}},{"type":"Binding site","location":{"start":{"value":489,"modifier":"EXACT"},"end":{"value":489,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q38802"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":621,"modifier":"EXACT"},"end":{"value":621,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q40577"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":621,"modifier":"EXACT"},"end":{"value":621,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q40577"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":625,"modifier":"EXACT"},"end":{"value":625,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q40577"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":625,"modifier":"EXACT"},"end":{"value":625,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q40577"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":765,"modifier":"EXACT"},"end":{"value":765,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q40577"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Binding site","location":{"start":{"value":769,"modifier":"EXACT"},"end":{"value":769,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q40577"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Binding site","location":{"start":{"value":773,"modifier":"EXACT"},"end":{"value":773,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q40577"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Mutagenesis","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"No effect on CPP binding, but decreased abietadiene synthase activity; when associated with A-87.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12614165"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"No effect on CPP binding, but decreased abietadiene synthase activity; when associated with A-86.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12614165"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":356,"modifier":"EXACT"},"end":{"value":356,"modifier":"EXACT"}},"description":"No effect on abietadiene synthase activity, but strongly reduced copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20430888"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":356,"modifier":"EXACT"},"end":{"value":356,"modifier":"EXACT"}},"description":"Increased Mg(2+) inhibition; when associated with A-621.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20430888"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["H"]}},{"type":"Mutagenesis","location":{"start":{"value":358,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"Decreased copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":361,"modifier":"EXACT"},"end":{"value":361,"modifier":"EXACT"}},"description":"No effect on GGPP binding, but decreased copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":365,"modifier":"EXACT"},"end":{"value":365,"modifier":"EXACT"}},"description":"No effect on GGPP binding, but decreased copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":402,"modifier":"EXACT"}},"description":"No effect on GGPP binding, but decreased copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A","E","N"]}},{"type":"Mutagenesis","location":{"start":{"value":404,"modifier":"EXACT"},"end":{"value":404,"modifier":"EXACT"}},"description":"No effect on GGPP binding, but loss of copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11552804"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A","E","N"]}},{"type":"Mutagenesis","location":{"start":{"value":405,"modifier":"EXACT"},"end":{"value":405,"modifier":"EXACT"}},"description":"No effect on GGPP binding, but decreased copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A","E","N"]}},{"type":"Mutagenesis","location":{"start":{"value":411,"modifier":"EXACT"},"end":{"value":411,"modifier":"EXACT"}},"description":"No effect on GGPP binding, but slightly decreased copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":451,"modifier":"EXACT"},"end":{"value":451,"modifier":"EXACT"}},"description":"No effect on abietadiene synthase activity, but strongly reduced copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22219188"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":454,"modifier":"EXACT"},"end":{"value":454,"modifier":"EXACT"}},"description":"No effect on GGPP binding, but decreased copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":499,"modifier":"EXACT"},"end":{"value":499,"modifier":"EXACT"}},"description":"No effect on GGPP binding, but decreased copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":520,"modifier":"EXACT"},"end":{"value":520,"modifier":"EXACT"}},"description":"No effect on GGPP binding, but slightly decreased copalyl diphosphate synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11827528"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":584,"modifier":"EXACT"},"end":{"value":584,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":586,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":589,"modifier":"EXACT"},"end":{"value":589,"modifier":"EXACT"}},"description":"Lower substrate binding and strong decrease of abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":617,"modifier":"EXACT"},"end":{"value":617,"modifier":"EXACT"}},"description":"Increased production of abietadiene at the expense of levopimaradiene.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":621,"modifier":"EXACT"},"end":{"value":621,"modifier":"EXACT"}},"description":"Loss of abietadiene synthase activity. Increased Mg(2+) inhibition; when associated with H-356.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11552804"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20430888"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":625,"modifier":"EXACT"},"end":{"value":625,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":699,"modifier":"EXACT"},"end":{"value":699,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":721,"modifier":"EXACT"},"end":{"value":721,"modifier":"EXACT"}},"description":"Lower substrate binding and strong decrease of abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":723,"modifier":"EXACT"},"end":{"value":723,"modifier":"EXACT"}},"description":"Produces pimaradienes instead of abietadienes.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18052062"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["S"]}},{"type":"Mutagenesis","location":{"start":{"value":727,"modifier":"EXACT"},"end":{"value":727,"modifier":"EXACT"}},"description":"No effect.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22219188"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["T"]}},{"type":"Mutagenesis","location":{"start":{"value":762,"modifier":"EXACT"},"end":{"value":762,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":765,"modifier":"EXACT"},"end":{"value":765,"modifier":"EXACT"}},"description":"Abolishes the conversion of CPP to abietadiene; produces only sandaracopimaradiene.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":766,"modifier":"EXACT"},"end":{"value":766,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":769,"modifier":"EXACT"},"end":{"value":769,"modifier":"EXACT"}},"description":"Increased production of neoabietadiene at the expense of both levopimaradiene and abietadiene.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":773,"modifier":"EXACT"},"end":{"value":773,"modifier":"EXACT"}},"description":"Increased production of neoabietadiene.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":778,"modifier":"EXACT"},"end":{"value":778,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":841,"modifier":"EXACT"},"end":{"value":841,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":845,"modifier":"EXACT"},"end":{"value":845,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":848,"modifier":"EXACT"},"end":{"value":848,"modifier":"EXACT"}},"description":"No or small effect on substrate binding, but strongly decreases abietadiene synthase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11805316"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83563","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["T"]}},{"type":"Sequence conflict","location":{"start":{"value":214,"modifier":"EXACT"},"end":{"value":214,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83563","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":297,"modifier":"EXACT"},"end":{"value":299,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83563","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"DWQ","alternativeSequences":["EWE"]}},{"type":"Sequence conflict","location":{"start":{"value":557,"modifier":"EXACT"},"end":{"value":557,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83563","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["L"]}},{"type":"Sequence conflict","location":{"start":{"value":579,"modifier":"EXACT"},"end":{"value":579,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83563","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["E"]}},{"type":"Sequence conflict","location":{"start":{"value":653,"modifier":"EXACT"},"end":{"value":653,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83563","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["K"]}},{"type":"Helix","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Beta strand","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Beta strand","location":{"start":{"value":179,"modifier":"EXACT"},"end":{"value":181,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":200,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":205,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":222,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":257,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":280,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":284,"modifier":"EXACT"},"end":{"value":291,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Turn","location":{"start":{"value":293,"modifier":"EXACT"},"end":{"value":295,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":298,"modifier":"EXACT"},"end":{"value":302,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":315,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":328,"modifier":"EXACT"},"end":{"value":341,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":352,"modifier":"EXACT"},"end":{"value":365,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":372,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":373,"modifier":"EXACT"},"end":{"value":384,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":403,"modifier":"EXACT"},"end":{"value":415,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":422,"modifier":"EXACT"},"end":{"value":428,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":446,"modifier":"EXACT"},"end":{"value":456,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":464,"modifier":"EXACT"},"end":{"value":480,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Turn","location":{"start":{"value":481,"modifier":"EXACT"},"end":{"value":483,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Beta strand","location":{"start":{"value":492,"modifier":"EXACT"},"end":{"value":494,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":496,"modifier":"EXACT"},"end":{"value":505,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":508,"modifier":"EXACT"},"end":{"value":510,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":513,"modifier":"EXACT"},"end":{"value":523,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Beta strand","location":{"start":{"value":530,"modifier":"EXACT"},"end":{"value":536,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Turn","location":{"start":{"value":539,"modifier":"EXACT"},"end":{"value":541,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":544,"modifier":"EXACT"},"end":{"value":574,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Turn","location":{"start":{"value":575,"modifier":"EXACT"},"end":{"value":578,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Beta strand","location":{"start":{"value":581,"modifier":"EXACT"},"end":{"value":583,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":587,"modifier":"EXACT"},"end":{"value":597,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":601,"modifier":"EXACT"},"end":{"value":603,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":604,"modifier":"EXACT"},"end":{"value":624,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":630,"modifier":"EXACT"},"end":{"value":642,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Beta strand","location":{"start":{"value":644,"modifier":"EXACT"},"end":{"value":647,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":648,"modifier":"EXACT"},"end":{"value":650,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":653,"modifier":"EXACT"},"end":{"value":677,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":682,"modifier":"EXACT"},"end":{"value":704,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":711,"modifier":"EXACT"},"end":{"value":721,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":724,"modifier":"EXACT"},"end":{"value":731,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Beta strand","location":{"start":{"value":735,"modifier":"EXACT"},"end":{"value":737,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":741,"modifier":"EXACT"},"end":{"value":744,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Turn","location":{"start":{"value":745,"modifier":"EXACT"},"end":{"value":747,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":752,"modifier":"EXACT"},"end":{"value":776,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":782,"modifier":"EXACT"},"end":{"value":789,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":795,"modifier":"EXACT"},"end":{"value":819,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":824,"modifier":"EXACT"},"end":{"value":840,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":851,"modifier":"EXACT"},"end":{"value":859,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]},{"type":"Helix","location":{"start":{"value":860,"modifier":"EXACT"},"end":{"value":864,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3S9V"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"8798524","citationType":"journal article","authors":["Stoffer Vogel B.","Wildung M.R.","Vogel G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"8798524"},{"database":"DOI","id":"10.1074/jbc.271.38.23262"}],"title":"Abietadiene synthase from grand fir (Abies grandis). cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase involved in resin acid biosynthesis.","publicationDate":"1996","journal":"J. Biol. Chem.","firstPage":"23262","lastPage":"23268","volume":"271"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","PROTEIN SEQUENCE OF 148-166; 522-530; 615-625 AND 699-705","INDUCTION"],"referenceComments":[{"value":"Stem","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-868","NOMENCLATURE"]},{"referenceNumber":3,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]},{"referenceNumber":4,"citation":{"id":"11112547","citationType":"journal article","authors":["Peters R.J.","Flory J.E.","Jetter R.","Ravn M.M.","Lee H.J.","Coates R.M.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11112547"},{"database":"DOI","id":"10.1021/bi001997l"}],"title":"Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the 'pseudomature' recombinant enzyme.","publicationDate":"2000","journal":"Biochemistry","firstPage":"15592","lastPage":"15602","volume":"39"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","ACTIVITY REGULATION"]},{"referenceNumber":5,"citation":{"id":"10814381","citationType":"journal article","authors":["Ravn M.M.","Coates R.M.","Flory J.E.","Peters R.J.","Croteau R."],"citationCrossReferences":[{"database":"PubMed","id":"10814381"},{"database":"DOI","id":"10.1021/ol991230p"}],"title":"Stereochemistry of the cyclization-rearrangement of (+)-copalyl diphosphate to (-)-abietadiene catalyzed by recombinant abietadiene synthase from Abies grandis.","publicationDate":"2000","journal":"Org. Lett.","firstPage":"573","lastPage":"576","volume":"2"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY"]},{"referenceNumber":6,"citation":{"id":"11552804","citationType":"journal article","authors":["Peters R.J.","Ravn M.M.","Coates R.M.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11552804"},{"database":"DOI","id":"10.1021/ja010670k"}],"title":"Bifunctional abietadiene synthase: free diffusive transfer of the (+)-copalyl diphosphate intermediate between two distinct active sites.","publicationDate":"2001","journal":"J. Am. Chem. Soc.","firstPage":"8974","lastPage":"8978","volume":"123"},"referencePositions":["MUTAGENESIS OF ASP-404 AND ASP-621","CHARACTERIZATION"]},{"referenceNumber":7,"citation":{"id":"11827528","citationType":"journal article","authors":["Peters R.J.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11827528"},{"database":"DOI","id":"10.1021/bi011879d"}],"title":"Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate.","publicationDate":"2002","journal":"Biochemistry","firstPage":"1836","lastPage":"1842","volume":"41"},"referencePositions":["FUNCTION","BIOPHYSICOCHEMICAL PROPERTIES","3D-STRUCTURE MODELING","MUTAGENESIS OF TRP-358; ASP-361; ARG-365; ASP-402; ASP-404; ASP-405; ARG-411; ARG-454; GLU-499 AND TYR-520"]},{"referenceNumber":8,"citation":{"id":"11805316","citationType":"journal article","authors":["Peters R.J.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11805316"},{"database":"DOI","id":"10.1073/pnas.022627099"}],"title":"Abietadiene synthase catalysis: mutational analysis of a prenyl diphosphate ionization-initiated cyclization and rearrangement.","publicationDate":"2002","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"580","lastPage":"584","volume":"99"},"referencePositions":["MUTAGENESIS OF ARG-584; ARG-586; GLU-589; THR-617; ASP-621; ASP-625; GLU-699; SER-721; ARG-762; ASN-765; ASP-766; THR-769; GLU-773; GLU-778; TYR-841; ASP-845 AND THR-848","CHARACTERIZATION"]},{"referenceNumber":9,"citation":{"id":"12614165","citationType":"journal article","authors":["Peters R.J.","Carter O.A.","Zhang Y.","Matthews B.W.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"12614165"},{"database":"DOI","id":"10.1021/bi020492n"}],"title":"Bifunctional abietadiene synthase: mutual structural dependence of the active sites for protonation-initiated and ionization-initiated cyclizations.","publicationDate":"2003","journal":"Biochemistry","firstPage":"2700","lastPage":"2707","volume":"42"},"referencePositions":["FUNCTION","MUTAGENESIS OF LYS-86 AND ARG-87"]},{"referenceNumber":10,"citation":{"id":"18052062","citationType":"journal article","authors":["Wilderman P.R.","Peters R.J."],"citationCrossReferences":[{"database":"PubMed","id":"18052062"},{"database":"DOI","id":"10.1021/ja074977g"}],"title":"A single residue switch converts abietadiene synthase into a pimaradiene specific cyclase.","publicationDate":"2007","journal":"J. Am. Chem. Soc.","firstPage":"15736","lastPage":"15737","volume":"129"},"referencePositions":["FUNCTION","MUTAGENESIS OF ALA-723"]},{"referenceNumber":11,"citation":{"id":"20430888","citationType":"journal article","authors":["Mann F.M.","Prisic S.","Davenport E.K.","Determan M.K.","Coates R.M.","Peters R.J."],"citationCrossReferences":[{"database":"PubMed","id":"20430888"},{"database":"DOI","id":"10.1074/jbc.m110.123307"}],"title":"A single residue switch for Mg(2+)-dependent inhibition characterizes plant class II diterpene cyclases from primary and secondary metabolism.","publicationDate":"2010","journal":"J. Biol. Chem.","firstPage":"20558","lastPage":"20563","volume":"285"},"referencePositions":["FUNCTION","MUTAGENESIS OF ARG-356 AND ASP-621"]},{"referenceNumber":12,"citation":{"id":"22219188","citationType":"journal article","authors":["Zhou K.","Gao Y.","Hoy J.A.","Mann F.M.","Honzatko R.B.","Peters R.J."],"citationCrossReferences":[{"database":"PubMed","id":"22219188"},{"database":"DOI","id":"10.1074/jbc.m111.337592"}],"title":"Insights into diterpene cyclization from structure of bifunctional abietadiene synthase from Abies grandis.","publicationDate":"2012","journal":"J. Biol. Chem.","firstPage":"6840","lastPage":"6850","volume":"287"},"referencePositions":["X-RAY CRYSTALLOGRAPHY","MUTAGENESIS OF ASN-451 AND VAL-727"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U50768","properties":[{"key":"ProteinId","value":"AAB05407.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF326516","properties":[{"key":"ProteinId","value":"AAK83563.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PDB","id":"3S9V","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.30 A"},{"key":"Chains","value":"A/B/C/D=85-868"}]},{"database":"AlphaFoldDB","id":"Q38710","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q38710","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAB05407","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-12822","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.132","properties":[{"key":"OrganismId","value":"2"}]},{"database":"BRENDA","id":"4.2.3.18","properties":[{"key":"OrganismId","value":"2"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"EvolutionaryTrace","id":"Q38710","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0050554","properties":[{"key":"GoTerm","value":"F:abietadiene synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0050559","properties":[{"key":"GoTerm","value":"F:copalyl diphosphate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010333","properties":[{"key":"GoTerm","value":"F:terpene synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000002","properties":[{"key":"EntryName","value":"Ent-copalyl diphosphate synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.160","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31739","properties":[{"key":"EntryName","value":"ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31739:SF4","properties":[{"key":"EntryName","value":"ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01604","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01605","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAMPSSSLSSQIPTAAHHLTANAQSIPHFSTTLNAGSSASKRRSLYLRWGKGSNKIIACVGEGGATSVPYQSAEKNDSLSSSTLVKREFPPGFWKDDLIDSLTSSHKVAASDEKRIETLISEIKNMFRCMGYGETNPSAYDTAWVARIPAVDGSDNPHFPETVEWILQNQLKDGSWGEGFYFLAYDRILATLACIITLTLWRTGETQVQKGIEFFRTQAGKMEDEADSHRPSGFEIVFPAMLKEAKILGLDLPYDLPFLKQIIEKREAKLKRIPTDVLYALPTTLLYSLEGLQEIVDWQKIMKLQSKDGSFLSSPASTAAVFMRTGNKKCLDFLNFVLKKFGNHVPCHYPLDLFERLWAVDTVERLGIDRHFKEEIKEALDYVYSHWDERGIGWARENPVPDIDDTAMGLRILRLHGYNVSSDVLKTFRDENGEFFCFLGQTQRGVTDMLNVNRCSHVSFPGETIMEEAKLCTERYLRNALENVDAFDKWAFKKNIRGEVEYALKYPWHKSMPRLEARSYIENYGPDDVWLGKTVYMMPYISNEKYLELAKLDFNKVQSIHQTELQDLRRWWKSSGFTDLNFTRERVTEIYFSPASFIFEPEFSKCREVYTKTSNFTVILDDLYDAHGSLDDLKLFTESVKRWDLSLVDQMPQQMKICFVGFYNTFNDIAKEGRERQGRDVLGYIQNVWKVQLEAYTKEAEWSEAKYVPSFNEYIENASVSIALGTVVLISALFTGEVLTDEVLSKIDRESRFLQLMGLTGRLVNDTKTYQAERGQGEVASAIQCYMKDHPKISEEEALQHVYSVMENALEELNREFVNNKIPDIYKRLVFETARIMQLFYMQGDGLTLSHDMEIKEHVKNCLFQPVA","length":868,"molWeight":99536,"crc64":"AD5E79F56B70D25C","md5":"61CB14B6BFFD209F9D60BE750AF95E03"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"COFACTOR":2,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":1,"PTM":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Motif":2,"Binding site":11,"Mutagenesis":34,"Sequence conflict":6,"Helix":40,"Beta strand":7,"Turn":5},"uniParcId":"UPI00000ACA58"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O24474","uniProtkbId":"TPSD2_ABIGR","entryAudit":{"firstPublicDate":"2001-10-24","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"1998-01-01","entryVersion":110,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Myrcene synthase, chloroplastic"},"ecNumbers":[{"value":"4.2.3.15"}]},"alternativeNames":[{"fullName":{"value":"Aggmyr"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"ag2"},"synonyms":[{"value":"AG2.2"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11404343"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-geranyl diphosphate = beta-myrcene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16965"},{"database":"ChEBI","id":"CHEBI:17221"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58057"}],"ecNumber":"4.2.3.15"}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 3 Mg(2+) or Mn(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9268308"}],"value":"By wounding"}],"commentType":"INDUCTION"},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":627,"modifier":"EXACT"}},"description":"Myrcene synthase, chloroplastic","featureId":"PRO_0000033627"},{"type":"Motif","location":{"start":{"value":378,"modifier":"EXACT"},"end":{"value":382,"modifier":"EXACT"}},"description":"DDXXD motif"},{"type":"Binding site","location":{"start":{"value":378,"modifier":"EXACT"},"end":{"value":378,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":378,"modifier":"EXACT"},"end":{"value":378,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":382,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":382,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":530,"modifier":"EXACT"},"end":{"value":530,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"9268308","citationType":"journal article","authors":["Bohlmann J.","Steele C.L.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9268308"},{"database":"DOI","id":"10.1074/jbc.272.35.21784"}],"title":"Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.","publicationDate":"1997","journal":"J. Biol. Chem.","firstPage":"21784","lastPage":"21792","volume":"272"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","INDUCTION","CHARACTERIZATION"]},{"referenceNumber":2,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]},{"referenceNumber":3,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U87908","properties":[{"key":"ProteinId","value":"AAB71084.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"O24474","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O24474","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAB71084","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-12818","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.15","properties":[{"key":"OrganismId","value":"2"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0050551","properties":[{"key":"GoTerm","value":"F:myrcene synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000002","properties":[{"key":"EntryName","value":"Ent-copalyl diphosphate synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31739:SF25","properties":[{"key":"EntryName","value":"(E,E)-GERANYLLINALOOL SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31739","properties":[{"key":"EntryName","value":"ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALVSISPLASKSCLRKSLISSIHEHKPPYRTIPNLGMRRRGKSVTPSMSISLATAAPDDGVQRRIGDYHSNIWDDDFIQSLSTPYGEPSYQERAERLIVEVKKIFNSMYLDDGRLMSSFNDLMQRLWIVDSVERLGIARHFKNEITSALDYVFRYWEENGIGCGRDSIVTDLNSTALGFRTLRLHGYTVSPEVLKAFQDQNGQFVCSPGQTEGEIRSVLNLYRASLIAFPGEKVMEEAEIFSTRYLKEALQKIPVSALSQEIKFVMEYGWHTNLPRLEARNYIDTLEKDTSAWLNKNAGKKLLELAKLEFNIFNSLQQKELQYLLRWWKESDLPKLTFARHRHVEFYTLASCIAIDPKHSAFRLGFAKMCHLVTVLDDIYDTFGTIDELELFTSAIKRWNSSEIEHLPEYMKCVYMVVFETVNELTREAEKTQGRNTLNYVRKAWEAYFDSYMEEAKWISNGYLPMFEEYHENGKVSSAYRVATLQPILTLNAWLPDYILKGIDFPSRFNDLASSFLRLRGDTRCYKADRDRGEEASCISCYMKDNPGSTEEDALNHINAMVNDIIKELNWELLRSNDNIPMLAKKHAFDITRALHHLYIYRDGFSVANKETKKLVMETLLESMLF","length":627,"molWeight":72479,"crc64":"2E0DA492E0C971FD","md5":"151BF35C8A9C21C6AD1A68A26347EB4B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Motif":1,"Binding site":5},"uniParcId":"UPI0000137743"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O64404","secondaryAccessions":["Q94FW3"],"uniProtkbId":"TPSD4_ABIGR","entryAudit":{"firstPublicDate":"2005-04-12","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"1998-08-01","entryVersion":101,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Delta-selinene synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}],"value":"4.2.3.71"},{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}],"value":"4.2.3.76"}]},"alternativeNames":[{"fullName":{"value":"Agfdsel1"}}]},"genes":[{"geneName":{"value":"ag4"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in 34 sesquiterpene (C15) olefins biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = (1E,4E)-germacrene B + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25444"},{"database":"ChEBI","id":"CHEBI:5337"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:175763"}],"ecNumber":"4.2.3.71","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = (+)-delta-selinene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:30423"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:49279"},{"database":"ChEBI","id":"CHEBI:175763"}],"ecNumber":"4.2.3.76","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = (+)-guaia-6,9-diene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25448"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:49204"},{"database":"ChEBI","id":"CHEBI:175763"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 3 Mg(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":1.5,"unit":"uM","substrate":"farnesyl diphosphate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}]}},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"value":"Constitutive expression"}],"commentType":"INDUCTION"},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"In sesquiterpene synthases utilizing farnesyl diphosphate as substrate, magnesium is more efficient as cofactor than is manganese"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":581,"modifier":"EXACT"}},"description":"Delta-selinene synthase","featureId":"PRO_0000186447"},{"type":"Motif","location":{"start":{"value":331,"modifier":"EXACT"},"end":{"value":335,"modifier":"EXACT"}},"description":"DDXXD motif"},{"type":"Binding site","location":{"start":{"value":331,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":331,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":335,"modifier":"EXACT"},"end":{"value":335,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":335,"modifier":"EXACT"},"end":{"value":335,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":475,"modifier":"EXACT"},"end":{"value":475,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Binding site","location":{"start":{"value":483,"modifier":"EXACT"},"end":{"value":483,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Sequence conflict","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["I"]}},{"type":"Sequence conflict","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"LTEMEMDDG","alternativeSequences":["SIEMEDS"]}},{"type":"Sequence conflict","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"QTA","alternativeSequences":["ETT"]}},{"type":"Sequence conflict","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["D"]}},{"type":"Sequence conflict","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["R"]}},{"type":"Sequence conflict","location":{"start":{"value":197,"modifier":"EXACT"},"end":{"value":197,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["T"]}},{"type":"Sequence conflict","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":203,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"AGH","alternativeSequences":["TGR"]}},{"type":"Sequence conflict","location":{"start":{"value":209,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"VDQ","alternativeSequences":["LDE"]}},{"type":"Sequence conflict","location":{"start":{"value":238,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["I"]}},{"type":"Sequence conflict","location":{"start":{"value":272,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["Q"]}},{"type":"Sequence conflict","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["D"]}},{"type":"Sequence conflict","location":{"start":{"value":295,"modifier":"EXACT"},"end":{"value":295,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["N"]}},{"type":"Sequence conflict","location":{"start":{"value":302,"modifier":"EXACT"},"end":{"value":307,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"SWVVMC","alternativeSequences":["FWAVIG"]}},{"type":"Sequence conflict","location":{"start":{"value":325,"modifier":"EXACT"},"end":{"value":326,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"IL","alternativeSequences":["TV"]}},{"type":"Sequence conflict","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["I"]}},{"type":"Sequence conflict","location":{"start":{"value":341,"modifier":"EXACT"},"end":{"value":343,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"HEI","alternativeSequences":["DEL"]}},{"type":"Sequence conflict","location":{"start":{"value":358,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"TD","alternativeSequences":["IH"]}},{"type":"Sequence conflict","location":{"start":{"value":375,"modifier":"EXACT"},"end":{"value":377,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"TVN","alternativeSequences":["IAD"]}},{"type":"Sequence conflict","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":381,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["F"]}},{"type":"Sequence conflict","location":{"start":{"value":386,"modifier":"EXACT"},"end":{"value":386,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["Q"]}},{"type":"Sequence conflict","location":{"start":{"value":393,"modifier":"EXACT"},"end":{"value":393,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":415,"modifier":"EXACT"},"end":{"value":415,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":418,"modifier":"EXACT"},"end":{"value":418,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["M"]}},{"type":"Sequence conflict","location":{"start":{"value":429,"modifier":"EXACT"},"end":{"value":429,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"M","alternativeSequences":["L"]}},{"type":"Sequence conflict","location":{"start":{"value":434,"modifier":"EXACT"},"end":{"value":434,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"M","alternativeSequences":["L"]}},{"type":"Sequence conflict","location":{"start":{"value":437,"modifier":"EXACT"},"end":{"value":437,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["V"]}},{"type":"Sequence conflict","location":{"start":{"value":446,"modifier":"EXACT"},"end":{"value":446,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["G"]}},{"type":"Sequence conflict","location":{"start":{"value":464,"modifier":"EXACT"},"end":{"value":467,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"LDLL","alternativeSequences":["QELS"]}},{"type":"Sequence conflict","location":{"start":{"value":474,"modifier":"EXACT"},"end":{"value":474,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["T"]}},{"type":"Sequence conflict","location":{"start":{"value":551,"modifier":"EXACT"},"end":{"value":551,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83561","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["L"]}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"9442047","citationType":"journal article","authors":["Steele C.L.","Crock J.","Bohlmann J.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9442047"},{"database":"DOI","id":"10.1074/jbc.273.4.2078"}],"title":"Sesquiterpene synthases from grand fir (Abies grandis). Comparison of constitutive and wound-induced activities, and cDNA isolation, characterization, and bacterial expression of delta-selinene synthase and gamma-humulene synthase.","publicationDate":"1998","journal":"J. Biol. Chem.","firstPage":"2078","lastPage":"2089","volume":"273"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES"]},{"referenceNumber":2,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","NOMENCLATURE"]},{"referenceNumber":3,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U92266","properties":[{"key":"ProteinId","value":"AAC05727.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF326513","properties":[{"key":"ProteinId","value":"AAK83561.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"O64404","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O64404","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAC05727","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:AG4-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.60","properties":[{"key":"OrganismId","value":"2"}]},{"database":"BRENDA","id":"4.2.3.71","properties":[{"key":"OrganismId","value":"2"}]},{"database":"BRENDA","id":"4.2.3.76","properties":[{"key":"OrganismId","value":"2"}]},{"database":"SABIO-RK","id":"O64404","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010333","properties":[{"key":"GoTerm","value":"F:terpene synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000002","properties":[{"key":"EntryName","value":"Ent-copalyl diphosphate synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31225","properties":[{"key":"EntryName","value":"OS04G0344100 PROTEIN-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAEISESSIPRRTGNHHGNVWDDDLIHSLNSPYGAPAYYELLQKLIQEIKHLLLTEMEMDDGDHDLIKRLQIVDTLECLGIDRHFEHEIQTAALDYVYRWWNEKGIGEGSRDSFSKDLNATALGFRALRLHRYNVSSGVLKNFKDENGKFFCNFTGEEGRGDKQVRSMLSLLRASEISFPGEKVMEEAKAFTREYLNQVLAGHGDVTDVDQSLLREVKYALEFPWHCSVPRWEARSFLEIYGHNHSWLKSNINQKMLKLAKLDFNILQCKHHKEIQFITRWWRDSGISQLNFYRKRHVEYYSWVVMCIFEPEFSESRIAFAKTAILCTVLDDLYDTHATLHEIKIMTEGVRRWDLSLTDDLPDYIKIAFQFFFNTVNELIVEIVKRQGRDMTTIVKDCWKRYIESYLQEAEWIATGHIPTFNEYIKNGMASSGMCILNLNPLLLLDKLLPDNILEQIHSPSKILDLLELTGRIADDLKDFEDEKERGEMASSLQCYMKENPESTVENALNHIKGILNRSLEEFNWEFMKQDSVPMCCKKFTFNIGRGLQFIYKYRDGLYISDKEVKDQIFKILVHQVPMEE","length":581,"molWeight":67968,"crc64":"864622AE8BCC32C8","md5":"A73CE231AA16990D2BE9C8207BF89B18"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"COFACTOR":2,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Motif":1,"Binding site":6,"Sequence conflict":32},"uniParcId":"UPI00000AB1A1"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O64405","uniProtkbId":"TPSD5_ABIGR","entryAudit":{"firstPublicDate":"2005-04-12","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"1998-08-01","entryVersion":101,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Gamma-humulene synthase"},"ecNumbers":[{"value":"4.2.3.56"}]},"alternativeNames":[{"fullName":{"value":"Agfghum"}}]},"genes":[{"geneName":{"value":"ag5"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11404343"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in 52 sesquiterpene (C15) olefins biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = gamma-humulene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25456"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:49290"},{"database":"ChEBI","id":"CHEBI:175763"}],"ecNumber":"4.2.3.56","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = sibirene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25460"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:49231"},{"database":"ChEBI","id":"CHEBI:175763"}],"ecNumber":"4.2.3.56","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = longifolene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25464"},{"database":"ChEBI","id":"CHEBI:6530"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:175763"}],"ecNumber":"4.2.3.56","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = beta-himachalene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25468"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:49210"},{"database":"ChEBI","id":"CHEBI:175763"}],"ecNumber":"4.2.3.56","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = gamma-himachalene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25472"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:49224"},{"database":"ChEBI","id":"CHEBI:175763"}],"ecNumber":"4.2.3.56","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = alpha-himachalene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25476"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:49214"},{"database":"ChEBI","id":"CHEBI:175763"}],"ecNumber":"4.2.3.56","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 3 Mg(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":4.5,"unit":"uM","substrate":"farnesyl diphosphate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9442047"}]}]}},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"value":"Constitutive expression"}],"commentType":"INDUCTION"},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"In sesquiterpene synthases utilizing farnesyl diphosphate as substrate, magnesium is more efficient as cofactor than is manganese"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":593,"modifier":"EXACT"}},"description":"Gamma-humulene synthase","featureId":"PRO_0000186448"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":343,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"DDXXD motif"},{"type":"Compositional bias","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":343,"modifier":"EXACT"},"end":{"value":343,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":343,"modifier":"EXACT"},"end":{"value":343,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":347,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":347,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":488,"modifier":"EXACT"},"end":{"value":488,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Binding site","location":{"start":{"value":496,"modifier":"EXACT"},"end":{"value":496,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"9442047","citationType":"journal article","authors":["Steele C.L.","Crock J.","Bohlmann J.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9442047"},{"database":"DOI","id":"10.1074/jbc.273.4.2078"}],"title":"Sesquiterpene synthases from grand fir (Abies grandis). Comparison of constitutive and wound-induced activities, and cDNA isolation, characterization, and bacterial expression of delta-selinene synthase and gamma-humulene synthase.","publicationDate":"1998","journal":"J. Biol. Chem.","firstPage":"2078","lastPage":"2089","volume":"273"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES"]},{"referenceNumber":2,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]},{"referenceNumber":3,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U92267","properties":[{"key":"ProteinId","value":"AAC05728.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"O64405","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O64405","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAC05728","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:AG5-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.56","properties":[{"key":"OrganismId","value":"2"}]},{"database":"SABIO-RK","id":"O64405","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010333","properties":[{"key":"GoTerm","value":"F:terpene synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31739:SF25","properties":[{"key":"EntryName","value":"(E,E)-GERANYLLINALOOL SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31739","properties":[{"key":"EntryName","value":"ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAQISESVSPSTDLKSTESSITSNRHGNMWEDDRIQSLNSPYGAPAYQERSEKLIEEIKLLFLSDMDDSCNDSDRDLIKRLEIVDTVECLGIDRHFQPEIKLALDYVYRCWNERGIGEGSRDSLKKDLNATALGFRALRLHRYNVSSGVLENFRDDNGQFFCGSTVEEEGAEAYNKHVRCMLSLSRASNILFPGEKVMEEAKAFTTNYLKKVLAGREATHVDESLLGEVKYALEFPWHCSVQRWEARSFIEIFGQIDSELKSNLSKKMLELAKLDFNILQCTHQKELQIISRWFADSSIASLNFYRKCYVEFYFWMAAAISEPEFSGSRVAFTKIAILMTMLDDLYDTHGTLDQLKIFTEGVRRWDVSLVEGLPDFMKIAFEFWLKTSNELIAEAVKAQGQDMAAYIRKNAWERYLEAYLQDAEWIATGHVPTFDEYLNNGTPNTGMCVLNLIPLLLMGEHLPIDILEQIFLPSRFHHLIELASRLVDDARDFQAEKDHGDLSCIECYLKDHPESTVEDALNHVNGLLGNCLLEMNWKFLKKQDSVPLSCKKYSFHVLARSIQFMYNQGDGFSISNKVIKDQVQKVLIVPVPI","length":593,"molWeight":67937,"crc64":"632E9EF069F21BD4","md5":"0F51CD8C75CA57235D255BB6B172A0B0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":6,"COFACTOR":2,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Motif":1,"Compositional bias":1,"Binding site":6},"uniParcId":"UPI000009D0DF"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O81086","secondaryAccessions":["Q94FW2","Q9SAU6"],"uniProtkbId":"TPSD1_ABIGR","entryAudit":{"firstPublicDate":"2005-04-12","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"1998-11-01","entryVersion":109,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Alpha-bisabolene synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9618485"}],"value":"4.2.3.38"}]},"alternativeNames":[{"fullName":{"value":"(E)-alpha-bisabolene synthase"}},{"fullName":{"value":"AgfEabis"}}]},"genes":[{"geneName":{"value":"ag1"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9618485"}],"value":"Converts farnesyl diphosphate to alpha-bisabolene (PubMed:22153510, PubMed:9539701, PubMed:9618485). Involved in defensive oleoresin formation in conifers in response to insect attack or other injury (PubMed:9539701, PubMed:9618485). Involved in sesquiterpene (C15) olefins biosynthesis (PubMed:9539701, PubMed:9618485)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E,6E)-farnesyl diphosphate = (E,R)-alpha-bisabolene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25436"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:49243"},{"database":"ChEBI","id":"CHEBI:175763"}],"ecNumber":"4.2.3.38","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9618485"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9618485"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9618485"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9618485"}],"value":"Binds 3 divalent metal cations per subunit (PubMed:22153510). Can use either Mg(2+) or Mn(2+) (PubMed:9618485)."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":49.5,"unit":"uM","substrate":"(2E,6E)-farnesyl diphosphate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"}]}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9618485"}],"value":"By wounding"}],"commentType":"INDUCTION"},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"The conserved 25-Arg-Arg-26 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":817,"modifier":"EXACT"}},"description":"Alpha-bisabolene synthase","featureId":"PRO_0000186446"},{"type":"Motif","location":{"start":{"value":566,"modifier":"EXACT"},"end":{"value":570,"modifier":"EXACT"}},"description":"DDXXD motif","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Binding site","location":{"start":{"value":566,"modifier":"EXACT"},"end":{"value":566,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SAE"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SDU"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":566,"modifier":"EXACT"},"end":{"value":566,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SAE"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SDU"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":570,"modifier":"EXACT"},"end":{"value":570,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SAE"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SDU"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":570,"modifier":"EXACT"},"end":{"value":570,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SAE"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SDU"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":713,"modifier":"EXACT"},"end":{"value":713,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SAE"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SDU"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Binding site","location":{"start":{"value":717,"modifier":"EXACT"},"end":{"value":717,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SAE"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SDU"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Binding site","location":{"start":{"value":721,"modifier":"EXACT"},"end":{"value":721,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SAE"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3SDU"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Mutagenesis","location":{"start":{"value":570,"modifier":"EXACT"},"end":{"value":570,"modifier":"EXACT"}},"description":"Abolishes catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":713,"modifier":"EXACT"},"end":{"value":713,"modifier":"EXACT"}},"description":"Abolishes catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"in Ref. 1; AAC24191 and 2; AAK83562","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["V"]}},{"type":"Sequence conflict","location":{"start":{"value":590,"modifier":"EXACT"},"end":{"value":590,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83562","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["V"]}},{"type":"Sequence conflict","location":{"start":{"value":655,"modifier":"EXACT"},"end":{"value":655,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83562","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":784,"modifier":"EXACT"},"end":{"value":784,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83562","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["G"]}},{"type":"Helix","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":90,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Beta strand","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":169,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":179,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":221,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":236,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Turn","location":{"start":{"value":238,"modifier":"EXACT"},"end":{"value":240,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Turn","location":{"start":{"value":243,"modifier":"EXACT"},"end":{"value":245,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":246,"modifier":"EXACT"},"end":{"value":249,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":273,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Beta strand","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":297,"modifier":"EXACT"},"end":{"value":310,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":314,"modifier":"EXACT"},"end":{"value":317,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":318,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":348,"modifier":"EXACT"},"end":{"value":360,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":367,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":387,"modifier":"EXACT"},"end":{"value":401,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":409,"modifier":"EXACT"},"end":{"value":426,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":429,"modifier":"EXACT"},"end":{"value":432,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":434,"modifier":"EXACT"},"end":{"value":438,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":441,"modifier":"EXACT"},"end":{"value":450,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Beta strand","location":{"start":{"value":453,"modifier":"EXACT"},"end":{"value":455,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDQ"}]},{"type":"Helix","location":{"start":{"value":458,"modifier":"EXACT"},"end":{"value":466,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Beta strand","location":{"start":{"value":475,"modifier":"EXACT"},"end":{"value":481,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Turn","location":{"start":{"value":484,"modifier":"EXACT"},"end":{"value":486,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":489,"modifier":"EXACT"},"end":{"value":519,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":522,"modifier":"EXACT"},"end":{"value":524,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":532,"modifier":"EXACT"},"end":{"value":540,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":546,"modifier":"EXACT"},"end":{"value":548,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":549,"modifier":"EXACT"},"end":{"value":567,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":568,"modifier":"EXACT"},"end":{"value":572,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":575,"modifier":"EXACT"},"end":{"value":587,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":590,"modifier":"EXACT"},"end":{"value":595,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":598,"modifier":"EXACT"},"end":{"value":622,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":627,"modifier":"EXACT"},"end":{"value":649,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":656,"modifier":"EXACT"},"end":{"value":666,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":669,"modifier":"EXACT"},"end":{"value":677,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":687,"modifier":"EXACT"},"end":{"value":691,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":701,"modifier":"EXACT"},"end":{"value":716,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":730,"modifier":"EXACT"},"end":{"value":737,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":743,"modifier":"EXACT"},"end":{"value":766,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Beta strand","location":{"start":{"value":769,"modifier":"EXACT"},"end":{"value":771,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDT"}]},{"type":"Helix","location":{"start":{"value":773,"modifier":"EXACT"},"end":{"value":789,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":800,"modifier":"EXACT"},"end":{"value":807,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":808,"modifier":"EXACT"},"end":{"value":812,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]},{"type":"Helix","location":{"start":{"value":813,"modifier":"EXACT"},"end":{"value":816,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3SDR"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"9618485","citationType":"journal article","authors":["Bohlmann J.","Crock J.","Jetter R.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9618485"},{"database":"DOI","id":"10.1073/pnas.95.12.6756"}],"title":"Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible (E)-alpha-bisabolene synthase from grand fir (Abies grandis).","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"6756","lastPage":"6761","volume":"95"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","FUNCTION","CATALYTIC ACTIVITY","COFACTOR","INDUCTION"]},{"referenceNumber":2,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-817","NOMENCLATURE"]},{"referenceNumber":3,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]},{"referenceNumber":4,"citation":{"id":"22153510","citationType":"journal article","authors":["McAndrew R.P.","Peralta-Yahya P.P.","DeGiovanni A.","Pereira J.H.","Hadi M.Z.","Keasling J.D.","Adams P.D."],"citationCrossReferences":[{"database":"PubMed","id":"22153510"},{"database":"DOI","id":"10.1016/j.str.2011.09.013"}],"title":"Structure of a three-domain sesquiterpene synthase: a prospective target for advanced biofuels production.","publicationDate":"2011","journal":"Structure","firstPage":"1876","lastPage":"1884","volume":"19"},"referencePositions":["X-RAY CRYSTALLOGRAPHY IN COMPLEX WITH MAGNESIUM","FUNCTION","CATALYTIC ACTIVITY","COFACTOR","BIOPHYSICOCHEMICAL PROPERTIES","MUTAGENESIS OF ASP-570 AND ASP-713"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF006195","properties":[{"key":"ProteinId","value":"AAC24192.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF006194","properties":[{"key":"ProteinId","value":"AAC24191.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF326515","properties":[{"key":"ProteinId","value":"AAK83562.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PDB","id":"3SAE","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.96 A"},{"key":"Chains","value":"A=1-817"}]},{"database":"PDB","id":"3SDQ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.14 A"},{"key":"Chains","value":"A=1-817"}]},{"database":"PDB","id":"3SDR","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.86 A"},{"key":"Chains","value":"A=1-817"}]},{"database":"PDB","id":"3SDT","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.89 A"},{"key":"Chains","value":"A=1-817"}]},{"database":"PDB","id":"3SDU","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.89 A"},{"key":"Chains","value":"A=1-817"}]},{"database":"PDB","id":"3SDV","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"A=1-817"}]},{"database":"AlphaFoldDB","id":"O81086","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O81086","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAC24192","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.38","properties":[{"key":"OrganismId","value":"2"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"EvolutionaryTrace","id":"O81086","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0052681","properties":[{"key":"GoTerm","value":"F:alpha-bisabolene synthase activity"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22153510"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9618485"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010333","properties":[{"key":"GoTerm","value":"F:terpene synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000002","properties":[{"key":"EntryName","value":"Ent-copalyl diphosphate synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.160","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31739:SF25","properties":[{"key":"EntryName","value":"(E,E)-GERANYLLINALOOL SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31739","properties":[{"key":"EntryName","value":"ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01604","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAGVSAVSKVSSLVCDLSSTSGLIRRTANPHPNVWGYDLVHSLKSPYIDSSYRERAEVLVSEIKAMLNPAITGDGESMITPSAYDTAWVARVPAIDGSARPQFPQTVDWILKNQLKDGSWGIQSHFLLSDRLLATLSCVLVLLKWNVGDLQVEQGIEFIKSNLELVKDETDQDSLVTDFEIIFPSLLREAQSLRLGLPYDLPYIHLLQTKRQERLAKLSREEIYAVPSPLLYSLEGIQDIVEWERIMEVQSQDGSFLSSPASTACVFMHTGDAKCLEFLNSVMIKFGNFVPCLYPVDLLERLLIVDNIVRLGIYRHFEKEIKEALDYVYRHWNERGIGWGRLNPIADLETTALGFRLLRLHRYNVSPAIFDNFKDANGKFICSTGQFNKDVASMLNLYRASQLAFPGENILDEAKSFATKYLREALEKSETSSAWNNKQNLSQEIKYALKTSWHASVPRVEAKRYCQVYRPDYARIAKCVYKLPYVNNEKFLELGKLDFNIIQSIHQEEMKNVTSWFRDSGLPLFTFARERPLEFYFLVAAGTYEPQYAKCRFLFTKVACLQTVLDDMYDTYGTLDELKLFTEAVRRWDLSFTENLPDYMKLCYQIYYDIVHEVAWEAEKEQGRELVSFFRKGWEDYLLGYYEEAEWLAAEYVPTLDEYIKNGITSIGQRILLLSGVLIMDGQLLSQEALEKVDYPGRRVLTELNSLISRLADDTKTYKAEKARGELASSIECYMKDHPECTEEEALDHIYSILEPAVKELTREFLKPDDVPFACKKMLFEETRVTMVIFKDGDGFGVSKLEVKDHIKECLIEPLPL","length":817,"molWeight":93749,"crc64":"95FB06DBC0DE1B4B","md5":"942EA94A28B8621896096CF8B0063258"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Motif":1,"Binding site":7,"Mutagenesis":2,"Sequence conflict":4,"Helix":46,"Beta strand":5,"Turn":3},"uniParcId":"UPI00000AA044"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O22340","secondaryAccessions":["Q94FV9"],"uniProtkbId":"TPSDA_ABIGR","entryAudit":{"firstPublicDate":"2001-10-24","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"1998-01-01","entryVersion":110,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Limonene synthase, chloroplastic"},"ecNumbers":[{"value":"4.2.3.16"}]},"alternativeNames":[{"fullName":{"value":"(-)-(4S)-limonene synthase"}},{"fullName":{"value":"Agg-lim1"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"ag10"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-geranyl diphosphate = (4S)-limonene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:12869"},{"database":"ChEBI","id":"CHEBI:15383"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58057"}],"ecNumber":"4.2.3.16"}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 3 Mg(2+) or Mn(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9268308"}],"value":"By wounding"}],"commentType":"INDUCTION"},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"The conserved 70-Arg-Arg-71 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":637,"modifier":"EXACT"}},"description":"Limonene synthase, chloroplastic","featureId":"PRO_0000033632"},{"type":"Motif","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":392,"modifier":"EXACT"}},"description":"DDXXD motif"},{"type":"Binding site","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":392,"modifier":"EXACT"},"end":{"value":392,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":392,"modifier":"EXACT"},"end":{"value":392,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":540,"modifier":"EXACT"},"end":{"value":540,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Sequence conflict","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["Q"]}},{"type":"Sequence conflict","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["D"]}},{"type":"Sequence conflict","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["L"]}},{"type":"Sequence conflict","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":207,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Sequence conflict","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["E"]}},{"type":"Sequence conflict","location":{"start":{"value":367,"modifier":"EXACT"},"end":{"value":367,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["E"]}},{"type":"Sequence conflict","location":{"start":{"value":441,"modifier":"EXACT"},"end":{"value":441,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Sequence conflict","location":{"start":{"value":455,"modifier":"EXACT"},"end":{"value":455,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["G"]}},{"type":"Sequence conflict","location":{"start":{"value":464,"modifier":"EXACT"},"end":{"value":464,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"M","alternativeSequences":["I"]}},{"type":"Sequence conflict","location":{"start":{"value":561,"modifier":"EXACT"},"end":{"value":561,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83565","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["T"]}}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"9268308","citationType":"journal article","authors":["Bohlmann J.","Steele C.L.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9268308"},{"database":"DOI","id":"10.1074/jbc.272.35.21784"}],"title":"Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.","publicationDate":"1997","journal":"J. Biol. Chem.","firstPage":"21784","lastPage":"21792","volume":"272"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","INDUCTION","CHARACTERIZATION"]},{"referenceNumber":2,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","NOMENCLATURE"]},{"referenceNumber":3,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF006193","properties":[{"key":"ProteinId","value":"AAB70907.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF326518","properties":[{"key":"ProteinId","value":"AAK83565.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"O22340","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O22340","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAB70907","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-12820","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.16","properties":[{"key":"OrganismId","value":"2"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0050552","properties":[{"key":"GoTerm","value":"F:(4S)-limonene synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000002","properties":[{"key":"EntryName","value":"Ent-copalyl diphosphate synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31739:SF25","properties":[{"key":"EntryName","value":"(E,E)-GERANYLLINALOOL SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31739","properties":[{"key":"EntryName","value":"ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALLSIVSLQVPKSCGLKSLISSSNVQKALCISTAVPTLRMRRRQKALVINMKLTTVSHRDDNGGGVLQRRIADHHPNLWEDDFIQSLSSPYGGSSYSERAETVVEEVKEMFNSIPNNRELFGSQNDLLTRLWMVDSIERLGIDRHFQNEIRVALDYVYSYWKEKEGIGCGRDSTFPDLNSTALALRTLRLHGYNVSSDVLEYFKDEKGHFACPAILTEGQITRSVLNLYRASLVAFPGEKVMEEAEIFSASYLKKVLQKIPVSNLSGEIEYVLEYGWHTNLPRLEARNYIEVYEQSGYESLNEMPYMNMKKLLQLAKLEFNIFHSLQLRELQSISRWWKESGSSQLTFTRHRHVEYYTMASCISMLPKHSAFRMEFVKVCHLVTVLDDIYDTFGTMNELQLFTDAIKRWDLSTTRWLPEYMKGVYMDLYQCINEMVEEAEKTQGRDMLNYIQNAWEALFDTFMQEAKWISSSYLPTFEEYLKNAKVSSGSRIATLQPILTLDVPLPDYILQEIDYPSRFNELASSILRLRGDTRCYKADRARGEEASAISCYMKDHPGSIEEDALNHINAMISDAIRELNWELLRPDSKSPISSKKHAFDITRAFHHVYKYRDGYTVSNNETKNLVMKTVLEPLAL","length":637,"molWeight":73478,"crc64":"8E80CD9DDE886898","md5":"4CD0D8781FEDE7EF96B6F4FC0A4DBA52"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Motif":1,"Binding site":5,"Sequence conflict":10},"uniParcId":"UPI0000137745"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9M7C9","uniProtkbId":"TPSDB_ABIGR","entryAudit":{"firstPublicDate":"2005-04-12","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"2000-10-01","entryVersion":95,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Limonene/alpha-pinene synthase, chloroplastic"}},"alternativeNames":[{"fullName":{"value":"(-)-(1S,5S)-alpha-pinene synthase"},"ecNumbers":[{"value":"4.2.3.119"}]},{"fullName":{"value":"(-)-(4S)-limonene synthase"},"ecNumbers":[{"value":"4.2.3.16"}]},{"fullName":{"value":"Agg-pin2"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"ag11"},"synonyms":[{"value":"agc11"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10441373"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11404343"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-geranyl diphosphate = (4S)-limonene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:12869"},{"database":"ChEBI","id":"CHEBI:15383"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58057"}],"ecNumber":"4.2.3.16","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10441373"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-geranyl diphosphate = (1S,5S)-alpha-pinene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25488"},{"database":"ChEBI","id":"CHEBI:28660"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58057"}],"ecNumber":"4.2.3.119","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10441373"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 3 Mg(2+) or Mn(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"value":"The C-terminal part of the protein seems to be involved in the cyclization step(s) required for the synthesis of pinene"}],"commentType":"DOMAIN"},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"The conserved 70-Arg-Arg-71 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":637,"modifier":"EXACT"}},"description":"Limonene/alpha-pinene synthase, chloroplastic","featureId":"PRO_0000033633"},{"type":"Motif","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":392,"modifier":"EXACT"}},"description":"DDXXD motif"},{"type":"Binding site","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":392,"modifier":"EXACT"},"end":{"value":392,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":392,"modifier":"EXACT"},"end":{"value":392,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":540,"modifier":"EXACT"},"end":{"value":540,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"10441373","citationType":"journal article","authors":["Bohlmann J.","Phillips M.","Ramachandiran V.","Katoh S.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"10441373"},{"database":"DOI","id":"10.1006/abbi.1999.1332"}],"title":"cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis).","publicationDate":"1999","journal":"Arch. Biochem. Biophys.","firstPage":"232","lastPage":"243","volume":"368"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","FUNCTION","CATALYTIC ACTIVITY"],"referenceComments":[{"value":"Stem","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]},{"referenceNumber":3,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF139207","properties":[{"key":"ProteinId","value":"AAF61455.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"Q9M7C9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9M7C9","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAF61455","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:AG11-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.119","properties":[{"key":"OrganismId","value":"2"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0050552","properties":[{"key":"GoTerm","value":"F:(4S)-limonene synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0050550","properties":[{"key":"GoTerm","value":"F:pinene synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000002","properties":[{"key":"EntryName","value":"Ent-copalyl diphosphate synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31739:SF25","properties":[{"key":"EntryName","value":"(E,E)-GERANYLLINALOOL SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31739","properties":[{"key":"EntryName","value":"ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALLSIVSLQVPKSCGLKSLISSSNVQKALCISTAVPTLRMRRRQKALVINMKLTTVSHRDDNGGGVLQRRIADHHPNLWEDDFIQSLSSPYGGSSYSERAVTVVEEVKEMFNSIPNNRELFGSQNDLLTRLWMVDSIERLGIDRHFQNEIRVALDYVYSYWKEKEGIGCGRDSTFPDLNSTALALRTLRLHGYNVSSDVLEYFKDQKGHFACPAILTEGQITRSVLNLYRASLVAFPGEKVMEEAEIFSASYLKEVLQKIPVSSFSREIEYVLEYGWHTNLPRLEARNYIDVYGQDSYESSNEMPYVNTQKLLKLAKLEFNIFHSLQQKELQYISRWWKDSCSSHLTFTRHRHVEYYTMASCISMEPKHSAFRLGFVKTCHLLTVLDDMYDTFGTLDELQLFTTAFKRWDLSETKCLPEYMKAVYMDLYQCLNELAQEAEKTQGRDTLNYIRNAYESHFDSFMHEAKWISSGYLPTFEEYLKNGKVSSGSRTATLQPILTLDVPLPNYILQEIDYPSRFNDLASSLLRLRGDTRCYKADRARGEEASAISCYMKDHPGSTEEDALNHINVMISDAIRELNWELLRPDSKSPISSKKHAFDITRAFHHLYKYRDGYTVASSETKNLVMKTVLEPVAL","length":637,"molWeight":73273,"crc64":"B3574986FEC96CFB","md5":"2370ACBE9D8007A7E21F3B38D94C9658"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"DOMAIN":2,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Motif":1,"Binding site":5},"uniParcId":"UPI00000A55BF"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O24475","secondaryAccessions":["Q94FW0"],"uniProtkbId":"TPSD3_ABIGR","entryAudit":{"firstPublicDate":"2001-10-24","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"1998-01-01","entryVersion":112,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Pinene synthase, chloroplastic"},"ecNumbers":[{"value":"4.2.3.119"},{"value":"4.2.3.120"}]},"alternativeNames":[{"fullName":{"value":"(-)-(1S,5S)-pinene synthase"}},{"fullName":{"value":"Agg-pin1"}},{"fullName":{"value":"Beta-geraniolene synthase"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"ag3"},"synonyms":[{"value":"AG3.18"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis. A mixture of alpha- and beta-pinene is produced by this enzyme"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-geranyl diphosphate = (1S,5S)-alpha-pinene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25488"},{"database":"ChEBI","id":"CHEBI:28660"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58057"}],"ecNumber":"4.2.3.119","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9268308"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-geranyl diphosphate = (1S,5S)-beta-pinene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25496"},{"database":"ChEBI","id":"CHEBI:28359"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58057"}],"ecNumber":"4.2.3.120","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9268308"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 3 Mg(2+) or Mn(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9268308"}],"value":"By wounding"}],"commentType":"INDUCTION"},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":628,"modifier":"EXACT"}},"description":"Pinene synthase, chloroplastic","featureId":"PRO_0000033628"},{"type":"Motif","location":{"start":{"value":379,"modifier":"EXACT"},"end":{"value":383,"modifier":"EXACT"}},"description":"DDXXD motif"},{"type":"Binding site","location":{"start":{"value":379,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":379,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":383,"modifier":"EXACT"},"end":{"value":383,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":383,"modifier":"EXACT"},"end":{"value":383,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":531,"modifier":"EXACT"},"end":{"value":531,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Sequence conflict","location":{"start":{"value":103,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83564","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["E"]}},{"type":"Sequence conflict","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83564","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["G"]}},{"type":"Sequence conflict","location":{"start":{"value":169,"modifier":"EXACT"},"end":{"value":169,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83564","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":180,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83564","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["F"]}},{"type":"Sequence conflict","location":{"start":{"value":286,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"in Ref. 2; AAK83564","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["H"]}}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"9268308","citationType":"journal article","authors":["Bohlmann J.","Steele C.L.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9268308"},{"database":"DOI","id":"10.1074/jbc.272.35.21784"}],"title":"Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.","publicationDate":"1997","journal":"J. Biol. Chem.","firstPage":"21784","lastPage":"21792","volume":"272"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","INDUCTION","CATALYTIC ACTIVITY","CHARACTERIZATION"]},{"referenceNumber":2,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-628","NOMENCLATURE"]},{"referenceNumber":3,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U87909","properties":[{"key":"ProteinId","value":"AAB71085.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF326517","properties":[{"key":"ProteinId","value":"AAK83564.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"O24475","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O24475","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAB71085","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-12819","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.119","properties":[{"key":"OrganismId","value":"2"}]},{"database":"BRENDA","id":"4.2.3.120","properties":[{"key":"OrganismId","value":"2"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0050550","properties":[{"key":"GoTerm","value":"F:pinene synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000004","properties":[{"key":"EntryName","value":"Carene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31225","properties":[{"key":"EntryName","value":"OS04G0344100 PROTEIN-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31225:SF137","properties":[{"key":"EntryName","value":"TERPENE SYNTHASE 11-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALVSTAPLASKSCLHKSLISSTHELKALSRTIPALGMSRRGKSITPSISMSSTTVVTDDGVRRRMGDFHSNLWDDDVIQSLPTAYEEKSYLERAEKLIGEVKNMFNSMSLEDGELMSPLNDLIQRLWIVDSLERLGIHRHFKDEIKSALDYVYSYWGENGIGCGRESVVTDLNSTALGLRTLRLHGYPVSSDVFKAFKGQNGQFSCSENIQTDEEIRGVLNLFRASLIAFPGEKIMDEAEIFSTKYLKEALQKIPVSSLSREIGDVLEYGWHTYLPRLEARNYIQVFGQDTENTKSYVKSKKLLELAKLEFNIFQSLQKRELESLVRWWKESGFPEMTFCRHRHVEYYTLASCIAFEPQHSGFRLGFAKTCHLITVLDDMYDTFGTVDELELFTATMKRWDPSSIDCLPEYMKGVYIAVYDTVNEMAREAEEAQGRDTLTYAREAWEAYIDSYMQEARWIATGYLPSFDEYYENGKVSCGHRISALQPILTMDIPFPDHILKEVDFPSKLNDLACAILRLRGDTRCYKADRARGEEASSISCYMKDNPGVSEEDALDHINAMISDVIKGLNWELLKPDINVPISAKKHAFDIARAFHYGYKYRDGYSVANVETKSLVTRTLLESVPL","length":628,"molWeight":71506,"crc64":"23DBB788F3C8072C","md5":"D93E0E535D9B30FA8FFEF226003CD454"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Motif":1,"Binding site":5,"Sequence conflict":5},"uniParcId":"UPI0000137742"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q948Z0","uniProtkbId":"TPSD6_ABIGR","entryAudit":{"firstPublicDate":"2005-04-12","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"2001-12-01","entryVersion":90,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Camphene synthase, chloroplastic"},"ecNumbers":[{"value":"4.2.3.117"}]},"alternativeNames":[{"fullName":{"value":"(-)-(1S,4R)-camphene synthase"}},{"fullName":{"value":"Agg-cam"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"ag6"},"synonyms":[{"value":"AG6.5"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10441373"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11404343"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-geranyl diphosphate = (1S,4R)-camphene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25484"},{"database":"ChEBI","id":"CHEBI:89"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58057"}],"ecNumber":"4.2.3.117","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10441373"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 3 Mg(2+) or Mn(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"The conserved 59-Arg-Arg-60 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":618,"modifier":"EXACT"}},"description":"Camphene synthase, chloroplastic","featureId":"PRO_0000033629"},{"type":"Motif","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"DDXXD motif"},{"type":"Binding site","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":373,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":373,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":521,"modifier":"EXACT"},"end":{"value":521,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"9268308","citationType":"journal article","authors":["Bohlmann J.","Steele C.L.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9268308"},{"database":"DOI","id":"10.1074/jbc.272.35.21784"}],"title":"Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.","publicationDate":"1997","journal":"J. Biol. Chem.","firstPage":"21784","lastPage":"21792","volume":"272"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"]},{"referenceNumber":2,"citation":{"id":"10441373","citationType":"journal article","authors":["Bohlmann J.","Phillips M.","Ramachandiran V.","Katoh S.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"10441373"},{"database":"DOI","id":"10.1006/abbi.1999.1332"}],"title":"cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis).","publicationDate":"1999","journal":"Arch. Biochem. Biophys.","firstPage":"232","lastPage":"243","volume":"368"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","FUNCTION","CATALYTIC ACTIVITY"],"referenceComments":[{"value":"Stem","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]},{"referenceNumber":4,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U87910","properties":[{"key":"ProteinId","value":"AAB70707.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"Q948Z0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q948Z0","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAB70707","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:AG6-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.117","properties":[{"key":"OrganismId","value":"2"}]},{"database":"BRENDA","id":"4.2.3.119","properties":[{"key":"OrganismId","value":"2"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0102703","properties":[{"key":"GoTerm","value":"F:camphene synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010333","properties":[{"key":"GoTerm","value":"F:terpene synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31225","properties":[{"key":"EntryName","value":"OS04G0344100 PROTEIN-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31225:SF137","properties":[{"key":"EntryName","value":"TERPENE SYNTHASE 11-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALLSITPLVSRSCLSSSHEIKALRRTIPTLGICRPGKSVAHSINMCLTSVASTDSVQRRVGNYHSNLWDDDFIQSLISTPYGAPDYRERADRLIGEVKDIMFNFKSLEDGGNDLLQRLLLVDDVERLGIDRHFKKEIKTALDYVNSYWNEKGIGCGRESVVTDLNSTALGLRTLRLHGYTVSSDVLNVFKDKNGQFSSTANIQIEGEIRGVLNLFRASLVAFPGEKVMDEAETFSTKYLREALQKIPASSILSLEIRDVLEYGWHTNLPRLEARNYMDVFGQHTKNKNAAEKLLELAKLEFNIFHSLQERELKHVSRWWKDSGSPEMTFCRHRHVEYYALASCIAFEPQHSGFRLGFTKMSHLITVLDDMYDVFGTVDELELFTATIKRWDPSAMECLPEYMKGVYMMVYHTVNEMARVAEKAQGRDTLNYARQAWEACFDSYMQEAKWIATGYLPTFEEYLENGKVSSAHRPCALQPILTLDIPFPDHILKEVDFPSKLNDLICIILRLRGDTRCYKADRARGEEASSISCYMKDNPGLTEEDALNHINFMIRDAIRELNWELLKPDNSVPITSKKHAFDISRVWHHGYRYRDGYSFANVETKSLVMRTVIEPVPL","length":618,"molWeight":70750,"crc64":"B07B5185CE5C4CE1","md5":"CB9526D300F50BE554025B85182E14FE"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Motif":1,"Binding site":5},"uniParcId":"UPI00000A90E0"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9M7D1","uniProtkbId":"TPSD8_ABIGR","entryAudit":{"firstPublicDate":"2005-04-12","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"2000-10-01","entryVersion":97,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Beta-phellandrene synthase, chloroplastic"},"ecNumbers":[{"value":"4.2.3.52"}]},"alternativeNames":[{"fullName":{"value":"(-)-(4S)-beta-phellandrene synthase"}},{"fullName":{"value":"Agg-Bphe"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"ag8"},"synonyms":[{"value":"agc8"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10441373"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11404343"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Converts geranyl diphosphate to four products with (-)-(4S)-beta-phellandrene (52%) as the major olefin, and lesser amounts of (-)-(1S,5S)-beta-pinene (34%), (-)-1S,5S-alpha-pinene (8.5%), and (-)-(4S)-limonene (6%). Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-geranyl diphosphate = (-)-beta-phellandrene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25492"},{"database":"ChEBI","id":"CHEBI:129"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58057"}],"ecNumber":"4.2.3.52","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10441373"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 3 Mg(2+) or Mn(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"The conserved 62-Arg-Arg-63 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":630,"modifier":"EXACT"}},"description":"Beta-phellandrene synthase, chloroplastic","featureId":"PRO_0000033630"},{"type":"Motif","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"DDXXD motif"},{"type":"Binding site","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":381,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":381,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":385,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":385,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":533,"modifier":"EXACT"},"end":{"value":533,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"10441373","citationType":"journal article","authors":["Bohlmann J.","Phillips M.","Ramachandiran V.","Katoh S.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"10441373"},{"database":"DOI","id":"10.1006/abbi.1999.1332"}],"title":"cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis).","publicationDate":"1999","journal":"Arch. Biochem. Biophys.","firstPage":"232","lastPage":"243","volume":"368"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","FUNCTION","CATALYTIC ACTIVITY"],"referenceComments":[{"value":"Stem","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]},{"referenceNumber":3,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF139205","properties":[{"key":"ProteinId","value":"AAF61453.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"Q9M7D1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9M7D1","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAF61453","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:AG8-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.52","properties":[{"key":"OrganismId","value":"2"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010333","properties":[{"key":"GoTerm","value":"F:terpene synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000002","properties":[{"key":"EntryName","value":"Ent-copalyl diphosphate synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31739:SF25","properties":[{"key":"EntryName","value":"(E,E)-GERANYLLINALOOL SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31739","properties":[{"key":"EntryName","value":"ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALVSSAPKSCLHKSLIRSTHHELKPLRRTIPTLGMCRRGKSFTPSVSMSLTTAVSDDGLQRRIGDYHSNLWDDDFIQSLSTPYGEPSYRERAEKLIGEVKEMFNSMPSEDGESMSPLNDLIERLWMVDSVERLGIDRHFKKEIKSALDYVYSYWNEKGIGCGRDSVFPDVNSTASGFRTLRLHGYSVSSEVLKVFQDQNGQFAFSPSTKERDIRTVLNLYRASFIAFPGEKVMEEAEIFSSRYLKEAVQKIPVSSLSQEIDYTLEYGWHTNMPRLETRNYLDVFGHPTSPWLKKKRTQYLDSEKLLELAKLEFNIFHSLQQKELQYLSRWWIHSGLPELTFGRHRHVEYYTLSSCIATEPKHSAFRLGFAKTCHLITVLDDIYDTFGTMDEIELFNEAVRRWNPSEKERLPEYMKEIYMALYEALTDMAREAEKTQGRDTLNYARKAWEVYLDSYTQEAKWIASGYLPTFEEYLENAKVSSGHRAAALTPLLTLDVPLPDDVLKGIDFPSRFNDLASSFLRLRGDTRCYKADRDRGEEASSISCYMKDNPGLTEEDALNHINAMINDIIKELNWELLKPDSNIPMTARKHAYEITRAFHQLYKYRDGFSVATQETKSLVRRTVLEPVPL","length":630,"molWeight":72784,"crc64":"B8E4374B262FF2D1","md5":"749A9CF6D7FC8C859A3D2F2E9583AC21"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Motif":1,"Binding site":5},"uniParcId":"UPI00000A9AD8"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9M7D0","uniProtkbId":"TPSD9_ABIGR","entryAudit":{"firstPublicDate":"2005-04-12","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"2000-10-01","entryVersion":96,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Terpinolene synthase, chloroplastic"},"ecNumbers":[{"value":"4.2.3.113"}]},"alternativeNames":[{"fullName":{"value":"Aggteo"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"ag9"},"synonyms":[{"value":"agc9"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10441373"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11404343"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9539701"}],"value":"Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-geranyl diphosphate = terpinolene + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25500"},{"database":"ChEBI","id":"CHEBI:9457"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58057"}],"ecNumber":"4.2.3.113","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10441373"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 3 Mg(2+) or Mn(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}]},{"texts":[{"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"value":"The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+)"}],"commentType":"DOMAIN"},{"texts":[{"value":"The conserved 65-Arg-Arg-66 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":630,"modifier":"EXACT"}},"description":"Terpinolene synthase, chloroplastic","featureId":"PRO_0000033631"},{"type":"Motif","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"DDXXD motif"},{"type":"Binding site","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":381,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":381,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":385,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":385,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":525,"modifier":"EXACT"},"end":{"value":525,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Binding site","location":{"start":{"value":533,"modifier":"EXACT"},"end":{"value":533,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"10441373","citationType":"journal article","authors":["Bohlmann J.","Phillips M.","Ramachandiran V.","Katoh S.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"10441373"},{"database":"DOI","id":"10.1006/abbi.1999.1332"}],"title":"cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis).","publicationDate":"1999","journal":"Arch. Biochem. Biophys.","firstPage":"232","lastPage":"243","volume":"368"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","FUNCTION","CATALYTIC ACTIVITY"],"referenceComments":[{"value":"Stem","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"9539701","citationType":"journal article","authors":["Bohlmann J.","Meyer-Gauen G.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"9539701"},{"database":"DOI","id":"10.1073/pnas.95.8.4126"}],"title":"Plant terpenoid synthases: molecular biology and phylogenetic analysis.","publicationDate":"1998","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4126","lastPage":"4133","volume":"95"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]},{"referenceNumber":3,"citation":{"id":"11404343","citationType":"journal article","authors":["Trapp S.C.","Croteau R.B."],"citationCrossReferences":[{"database":"PubMed","id":"11404343"},{"database":"DOI","id":"10.1093/genetics/158.2.811"}],"title":"Genomic organization of plant terpene synthases and molecular evolutionary implications.","publicationDate":"2001","journal":"Genetics","firstPage":"811","lastPage":"832","volume":"158"},"referencePositions":["GENE FAMILY","NOMENCLATURE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF139206","properties":[{"key":"ProteinId","value":"AAF61454.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"Q9M7D0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9M7D0","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ag:AAF61454","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:AG9-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.113","properties":[{"key":"OrganismId","value":"2"}]},{"database":"BRENDA","id":"4.2.3.119","properties":[{"key":"OrganismId","value":"2"}]},{"database":"BRENDA","id":"4.2.3.120","properties":[{"key":"OrganismId","value":"2"}]},{"database":"BRENDA","id":"4.2.3.16","properties":[{"key":"OrganismId","value":"2"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010333","properties":[{"key":"GoTerm","value":"F:terpene synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000004","properties":[{"key":"EntryName","value":"Carene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31225","properties":[{"key":"EntryName","value":"OS04G0344100 PROTEIN-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31225:SF137","properties":[{"key":"EntryName","value":"TERPENE SYNTHASE 11-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALVSILPLSSKSVLHKSWIVSTYEHKAISRTIPNLGLRGRGKSVTHSLRMSLSTAVSDDHGVQRRIVEFHSNLWDDDFIQSLSTPYGAPSYRERADRLIVEVKGIFTSISAEDGELITPLNDLIQRLLMVDNVERLGIDRHFKNEIKAALDYVYSYWNEKGIGSGSDSGVADLNSTALGFRILRLHGYSVSSDVLEHFKEEKEKGQFVCSAIQTEEEIKSVLNLFRASLIAFPGEKVMEEAEIFSKIYLKEALQNIAVSSLSREIEYVLEDGWQTNMPRLETRNYIDVLGENDRDETLYMNMEKLLEIAKLEFNIFHSLQQRELKDLSRWWKDSGFSHLTFSRHRHVEFYALASCIETDRKHSGFRLGFAKMCHLITVLDDIYDTFGTMEELELFTAAFKRWDPSATDLLPEYMKGLYMVVYETVNEIAREADKSQGRETLNDARRAWEAYLDSYMKEAEWISSGYLPTFEEYMETSKVSFGYRIFALQPILTMDVPLTHHILQEIDFPLRFNDLICSILRLKNDTRCYKADRARGEEASCISCYMKENPGSTEEDAINHINAMVNNLIKEVNWELLRQDGTAHIACKKHAFDILKGSLHGYKYRDGFSVANKETKNWVRRTVLESVPL","length":630,"molWeight":72508,"crc64":"452437B87F203D8A","md5":"31E5D227B3C64B4AE6FB2A2271A68250"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Motif":1,"Binding site":6},"uniParcId":"UPI00000A0DCF"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"G9JUE9","uniProtkbId":"G9JUE9_ABIGR","entryAudit":{"firstPublicDate":"2012-02-22","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2012-02-22","entryVersion":40,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW90991.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00017725"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"value":"Ribulose bisphosphate carboxylase large chain"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012287"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"value":"4.1.1.39"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW90991.1"}],"value":"rbcL"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00025664"}],"value":"RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 (2R)-3-phosphoglycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO2 + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23124"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57870"},{"database":"ChEBI","id":"CHEBI:58272"}],"ecNumber":"4.1.1.39","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049469"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10114"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-ribulose 1,5-bisphosphate + O2 = 2-phosphoglycolate + (2R)-3-phosphoglycerate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:36631"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57870"},{"database":"ChEBI","id":"CHEBI:58033"},{"database":"ChEBI","id":"CHEBI:58272"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048059"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"value":"Binds 1 Mg(2+) ion per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00025888"}],"value":"Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004229"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006204"}],"value":"Belongs to the RuBisCO large chain family. Type I subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"Ribulose bisphosphate carboxylase large subunit ferrodoxin-like N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02788"}]},{"type":"Domain","location":{"start":{"value":135,"modifier":"EXACT"},"end":{"value":420,"modifier":"EXACT"}},"description":"Ribulose bisphosphate carboxylase large subunit C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00016"}]},{"type":"Binding site","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10114"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Modified residue","location":{"start":{"value":182,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"N6-carboxylysine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10114"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW90991.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":422,"modifier":"EXACT"},"end":{"value":422,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW90991.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW90991.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022567"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0113","category":"Biological process","name":"Calvin cycle"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023300"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0120","category":"Biological process","name":"Carbon dioxide fixation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022481"}],"id":"KW-0488","category":"PTM","name":"Methylation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023033"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0503","category":"Molecular function","name":"Monooxygenase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023238"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0601","category":"Biological process","name":"Photorespiration"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022531"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000302"}],"id":"KW-0602","category":"Biological process","name":"Photosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022640"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW90991.1"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"}],"references":[{"referenceNumber":1,"citation":{"id":"22019929","citationType":"journal article","authors":["Aguirre-Planter E.","Jaramillo-Correa J.P.","Gomez-Acevedo S.","Khasa D.P.","Bousquet J.","Eguiarte L.E."],"citationCrossReferences":[{"database":"PubMed","id":"22019929"},{"database":"DOI","id":"10.1016/j.ympev.2011.09.021"}],"title":"Phylogeny, diversification rates and species boundaries of Mesoamerican firs (Abies, Pinaceae) in a genus-wide context.","publicationDate":"2012","journal":"Mol. Phylogenet. Evol.","firstPage":"263","lastPage":"274","volume":"62"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW90991.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"JN935609","properties":[{"key":"ProteinId","value":"AEW90991.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016984","properties":[{"key":"GoTerm","value":"F:ribulose-bisphosphate carboxylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0015977","properties":[{"key":"GoTerm","value":"P:carbon fixation"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd08212","properties":[{"key":"EntryName","value":"RuBisCO_large_I"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.110:FF:000003","properties":[{"key":"EntryName","value":"Ribulose bisphosphate carboxylase large chain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.110","properties":[{"key":"EntryName","value":"Ribulose bisphosphate carboxylase, large subunit, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.150","properties":[{"key":"EntryName","value":"RuBisCO large subunit, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01338","properties":[{"key":"EntryName","value":"RuBisCO_L_type1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR033966","properties":[{"key":"EntryName","value":"RuBisCO"}]},{"database":"InterPro","id":"IPR020878","properties":[{"key":"EntryName","value":"RuBisCo_large_chain_AS"}]},{"database":"InterPro","id":"IPR000685","properties":[{"key":"EntryName","value":"RuBisCO_lsu_C"}]},{"database":"InterPro","id":"IPR036376","properties":[{"key":"EntryName","value":"RuBisCO_lsu_C_sf"}]},{"database":"InterPro","id":"IPR017443","properties":[{"key":"EntryName","value":"RuBisCO_lsu_fd_N"}]},{"database":"InterPro","id":"IPR036422","properties":[{"key":"EntryName","value":"RuBisCO_lsu_N_sf"}]},{"database":"InterPro","id":"IPR020888","properties":[{"key":"EntryName","value":"RuBisCO_lsuI"}]},{"database":"NCBIfam","id":"NF003252","properties":[{"key":"EntryName","value":"PRK04208.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42704","properties":[{"key":"EntryName","value":"RIBULOSE BISPHOSPHATE CARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42704:SF17","properties":[{"key":"EntryName","value":"RIBULOSE BISPHOSPHATE CARBOXYLASE LARGE CHAIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00016","properties":[{"key":"EntryName","value":"RuBisCO_large"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02788","properties":[{"key":"EntryName","value":"RuBisCO_large_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01052","properties":[{"key":"EntryName","value":"RuBisCO"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00014","properties":[{"key":"EntryName","value":"RuBisCO"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG00301","properties":[{"key":"EntryName","value":"RuBisCO-like_proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51649","properties":[{"key":"EntryName","value":"RuBisCo, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54966","properties":[{"key":"EntryName","value":"RuBisCO, large subunit, small (N-terminal) domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00157","properties":[{"key":"EntryName","value":"RUBISCO_LARGE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"YRLTYYTPEYQTKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYDIEPVPGEESQFIAYVAYPLDLFEEGSVTNLFTSIVGNVFGFKALRALRLEDLRIPPAYSKTFQGPPHGIQVERDKXNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFVFCAEALYKAQAETGEIKGHYLNATAGTCEEMMKRAVFARELGVPIVMHDYLTGGFTANTTLAHYCRDNGLLLHIHRAMHAVIDRQRNHGMHFRVLAKALRMSGGDHIHAGTVVGKLEGERDVTLGFVDLLRDDFIEKDRSRGIFFTQDWVSMPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAAANRVALEACVQARNEGRDLAREG","length":422,"molWeight":46731,"crc64":"72FD0D3F0F16B703","md5":"93DA7AA9D12B17607F38538F18B9B79D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Binding site":1,"Modified residue":1,"Non-terminal residue":2},"uniParcId":"UPI000243F112"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A386GYG9","uniProtkbId":"A0A386GYG9_ABIGR","entryAudit":{"firstPublicDate":"2018-12-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2018-12-05","entryVersion":13,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AYD38172.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01390"}],"value":"Maturase K"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01390"}],"value":"Intron maturase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01390"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AYD38172.1"}],"value":"matK"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01390"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004226"}],"value":"Usually encoded in the trnK tRNA gene intron. Probably assists in splicing its own and other chloroplast group II introns"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01390"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006621"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01390"}],"value":"Belongs to the intron maturase 2 family. MatK subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":330,"modifier":"EXACT"}},"description":"Maturase MatK N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01824"}]},{"type":"Domain","location":{"start":{"value":360,"modifier":"EXACT"},"end":{"value":468,"modifier":"EXACT"}},"description":"Domain X","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01348"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AYD38172.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004226"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AYD38172.1"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022664"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01390"}],"id":"KW-0507","category":"Biological process","name":"mRNA processing"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022640"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004226"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022884"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01390"}],"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022694"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01390"}],"id":"KW-0819","category":"Biological process","name":"tRNA processing"}],"references":[{"referenceNumber":1,"citation":{"id":"30157290","citationType":"journal article","authors":["Leslie A.B.","Beaulieu J.","Holman G.","Campbell C.S.","Mei W.","Raubeson L.R.","Mathews S."],"citationCrossReferences":[{"database":"PubMed","id":"30157290"},{"database":"DOI","id":"10.1002/ajb2.1143"}],"title":"An overview of extant conifer evolution from the perspective of the fossil record.","publicationDate":"2018","journal":"Am. J. Bot.","firstPage":"1531","lastPage":"1544","volume":"105"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AYD38172.1"}],"value":"CSC_AA1473_81D","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AYD38172.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"MH230846","properties":[{"key":"ProteinId","value":"AYD38172.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A386GYG9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0006397","properties":[{"key":"GoTerm","value":"P:mRNA processing"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008380","properties":[{"key":"GoTerm","value":"P:RNA splicing"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_01390","properties":[{"key":"EntryName","value":"MatK"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR024937","properties":[{"key":"EntryName","value":"Domain_X"}]},{"database":"InterPro","id":"IPR002866","properties":[{"key":"EntryName","value":"Maturase_MatK"}]},{"database":"InterPro","id":"IPR024942","properties":[{"key":"EntryName","value":"Maturase_MatK_N"}]},{"database":"PANTHER","id":"PTHR34811","properties":[{"key":"EntryName","value":"MATURASE K"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR34811:SF1","properties":[{"key":"EntryName","value":"MATURASE K"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01348","properties":[{"key":"EntryName","value":"Intron_maturas2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01824","properties":[{"key":"EntryName","value":"MatK_N"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDEFHRYGKEDSSWQQCFLYPLFFQEDLYAICHDHYLDGSSSSESMEHLSSNDQFSFLTVKRLIGQIRQQNNSIVFFLNCDPNPLVDRNKSFYYESVLEGLTLVLEVPFSIRSKYSVEGMNDWKSFRSIHSIFPFLEDKFPHSNYILDTRIPYSIHPEILVRTFRRWIRDAPSLHPLRSVLYKYRNSPDNLQRSIIIAPRVNTRFLLFLWNHYVYGCESILVPLLKRSFHPRSSSHGSFPDRTHFDRKIKHIIINSRRNSLKSIWSLKDPRIHYVRYAERSIIAIKGTHLLVKKCRYHLPIFRQFYFHLWSEPYRVCSHQLSKNCSSSLGYFLRVRMKPLLVRTKMLDDLFITDLITDEFDPIVPIVPIIGLLAREKLCDISGRPISKLYWTSLTDDDILDRFDRIWKNIFHYYSGSLDRDGLYRIKYILSLSCAKTLACKHKSTIRVVRKELGPELFKKYFSKEREFDFPAFSSKAAARSQRERIWHSDIPQINPLANSWQKIQDLKS","length":509,"molWeight":60452,"crc64":"B8EB162893898C57","md5":"1AA9042BB5B5B06001893699BB229299"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2},"uniParcId":"UPI000EB711C7"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"G9JUK8","uniProtkbId":"G9JUK8_ABIGR","entryAudit":{"firstPublicDate":"2012-02-22","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2012-02-22","entryVersion":40,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91050.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00035266"}],"value":"Small ribosomal subunit protein bS18c"}},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91050.1"}],"value":"rpS18"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AGL76236.1"}],"value":"rps18"}]}],"comments":[{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004229"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005589"}],"value":"Belongs to the bacterial ribosomal protein bS18 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91050.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91050.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91050.1"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022640"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91050.1"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023274"}],"id":"KW-0687","category":"Molecular function","name":"Ribonucleoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022980"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91050.1"}],"id":"KW-0689","category":"Molecular function","name":"Ribosomal protein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022884"}],"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022730"}],"id":"KW-0699","category":"Molecular function","name":"rRNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"22019929","citationType":"journal article","authors":["Aguirre-Planter E.","Jaramillo-Correa J.P.","Gomez-Acevedo S.","Khasa D.P.","Bousquet J.","Eguiarte L.E."],"citationCrossReferences":[{"database":"PubMed","id":"22019929"},{"database":"DOI","id":"10.1016/j.ympev.2011.09.021"}],"title":"Phylogeny, diversification rates and species boundaries of Mesoamerican firs (Abies, Pinaceae) in a genus-wide context.","publicationDate":"2012","journal":"Mol. Phylogenet. Evol.","firstPage":"263","lastPage":"274","volume":"62"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91050.1"}]},{"referenceNumber":2,"citation":{"id":"CI-5AHJ7A7VAG9D6","citationType":"submission","authors":["Semerikova S.A.","Semerikov V.L."],"title":"Phylogeny and biogeography of Abies based on chloroplast DNA.","publicationDate":"FEB-2013","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AGL76238.1"}],"value":"26-2011","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AGL76236.1"}]},{"referenceNumber":3,"citation":{"id":"CI-76LTL2ESK7OIS","citationType":"submission","authors":["Semerikova S."],"title":"Nuclear multi-gene phylogeny of Abies.","publicationDate":"DEC-2016","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQZ40186.1"}],"value":"21-2013","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQZ40186.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"JN935717","properties":[{"key":"ProteinId","value":"AEW91050.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KC597673","properties":[{"key":"ProteinId","value":"AGL76236.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KC597674","properties":[{"key":"ProteinId","value":"AGL76238.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KY382171","properties":[{"key":"ProteinId","value":"AQZ40186.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"G9JUK8","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005840","properties":[{"key":"GoTerm","value":"C:ribosome"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003735","properties":[{"key":"GoTerm","value":"F:structural constituent of ribosome"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006412","properties":[{"key":"GoTerm","value":"P:translation"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"4.10.640.10","properties":[{"key":"EntryName","value":"Ribosomal protein S18"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001648","properties":[{"key":"EntryName","value":"Ribosomal_bS18"}]},{"database":"InterPro","id":"IPR036870","properties":[{"key":"EntryName","value":"Ribosomal_bS18_sf"}]},{"database":"PANTHER","id":"PTHR13479","properties":[{"key":"EntryName","value":"30S RIBOSOMAL PROTEIN S18"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13479:SF40","properties":[{"key":"EntryName","value":"SMALL RIBOSOMAL SUBUNIT PROTEIN BS18M"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01084","properties":[{"key":"EntryName","value":"Ribosomal_S18"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00974","properties":[{"key":"EntryName","value":"RIBOSOMALS18"}]},{"database":"SUPFAM","id":"SSF46911","properties":[{"key":"EntryName","value":"Ribosomal protein S18"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"GKILSGRVNRLTSKQQRLMTNAIKRARILSLLPFLYNEN","length":39,"molWeight":4514,"crc64":"D7CDCA40E7F96A66","md5":"BEFFD6F458ECE32D723CD72F61516A12"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Non-terminal residue":1},"uniParcId":"UPI000008BCF4"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"G9JUK9","uniProtkbId":"G9JUK9_ABIGR","entryAudit":{"firstPublicDate":"2012-02-22","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2012-02-22","entryVersion":27,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91051.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00035295"}],"value":"Large ribosomal subunit protein bL20c"}},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91051.1"}],"value":"rpl20"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007698"}],"value":"Belongs to the bacterial ribosomal protein bL20 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91051.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91051.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91051.1"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91051.1"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023274"}],"id":"KW-0687","category":"Molecular function","name":"Ribonucleoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022980"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91051.1"}],"id":"KW-0689","category":"Molecular function","name":"Ribosomal protein"}],"references":[{"referenceNumber":1,"citation":{"id":"22019929","citationType":"journal article","authors":["Aguirre-Planter E.","Jaramillo-Correa J.P.","Gomez-Acevedo S.","Khasa D.P.","Bousquet J.","Eguiarte L.E."],"citationCrossReferences":[{"database":"PubMed","id":"22019929"},{"database":"DOI","id":"10.1016/j.ympev.2011.09.021"}],"title":"Phylogeny, diversification rates and species boundaries of Mesoamerican firs (Abies, Pinaceae) in a genus-wide context.","publicationDate":"2012","journal":"Mol. Phylogenet. Evol.","firstPage":"263","lastPage":"274","volume":"62"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEW91051.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"JN935717","properties":[{"key":"ProteinId","value":"AEW91051.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"G9JUK9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005840","properties":[{"key":"GoTerm","value":"C:ribosome"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0019843","properties":[{"key":"GoTerm","value":"F:rRNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003735","properties":[{"key":"GoTerm","value":"F:structural constituent of ribosome"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006412","properties":[{"key":"GoTerm","value":"P:translation"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"1.10.1900.20","properties":[{"key":"EntryName","value":"Ribosomal protein L20"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005813","properties":[{"key":"EntryName","value":"Ribosomal_bL20"}]},{"database":"InterPro","id":"IPR035566","properties":[{"key":"EntryName","value":"Ribosomal_protein_bL20_C"}]},{"database":"PANTHER","id":"PTHR10986","properties":[{"key":"EntryName","value":"39S RIBOSOMAL PROTEIN L20"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00453","properties":[{"key":"EntryName","value":"Ribosomal_L20"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF74731","properties":[{"key":"EntryName","value":"Ribosomal protein L20"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"NGVSYNRFIQYLYKRQLLPNRKTLAQIAVLDSNCFSTILKKELIV","length":45,"molWeight":5273,"crc64":"96578AD2022C402B","md5":"A3BD32BED027E560840A0398BCA637D1"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Non-terminal residue":1},"uniParcId":"UPI000243F4D0"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q8LKJ1","uniProtkbId":"Q8LKJ1_ABIGR","entryAudit":{"firstPublicDate":"2002-10-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2002-10-01","entryVersion":74,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01135.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01135.1"}],"value":"Geranyl diphosphate synthase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01135.1"}],"value":"GPPS3"}}],"comments":[{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005128"}],"value":"Isoprenoid biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006706"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004466"}],"value":"Belongs to the FPP/GGPP synthase family"}],"commentType":"SIMILARITY"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023229"}],"id":"KW-0414","category":"Biological process","name":"Isoprene biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004466"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"12176066","citationType":"journal article","authors":["Burke C.","Croteau R."],"citationCrossReferences":[{"database":"PubMed","id":"12176066"},{"database":"DOI","id":"10.1016/S0003-9861(02)00335-1"}],"title":"Geranyl diphosphate synthase from Abies grandis: cDNA isolation, functional expression, and characterization.","publicationDate":"2002","journal":"Arch. Biochem. Biophys.","firstPage":"130","lastPage":"136","volume":"405"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01135.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF513113","properties":[{"key":"ProteinId","value":"AAN01135.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"Q8LKJ1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0004659","properties":[{"key":"GoTerm","value":"F:prenyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008299","properties":[{"key":"GoTerm","value":"P:isoprenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00685","properties":[{"key":"EntryName","value":"Trans_IPPS_HT"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000001","properties":[{"key":"EntryName","value":"Geranylgeranyl diphosphate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR000092","properties":[{"key":"EntryName","value":"Polyprenyl_synt"}]},{"database":"InterPro","id":"IPR033749","properties":[{"key":"EntryName","value":"Polyprenyl_synt_CS"}]},{"database":"InterPro","id":"IPR053378","properties":[{"key":"EntryName","value":"Prenyl_diphosphate_synthase"}]},{"database":"NCBIfam","id":"NF045485","properties":[{"key":"EntryName","value":"FPPsyn"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43281","properties":[{"key":"EntryName","value":"FARNESYL DIPHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43281:SF1","properties":[{"key":"EntryName","value":"FARNESYL DIPHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00348","properties":[{"key":"EntryName","value":"polyprenyl_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01017","properties":[{"key":"EntryName","value":"Polyprenyl_Transferase_Like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00723","properties":[{"key":"EntryName","value":"POLYPRENYL_SYNTHASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00444","properties":[{"key":"EntryName","value":"POLYPRENYL_SYNTHASE_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAYSGMVRSFQGVYFMAVALDRNRNLKRIDIPSKRFDGVSTSFVACNGEHLGLPVNLKKEFLSCIQRASSSRSSNTIVQFANLPEQGKKVVEFDFNKYMLSKAMAVTEALDKAIPHSYPQKIHESMRYSLLAGGKRVRPVLCIAACELVGGREELAMPTACAMEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGQDTALLAGDALHAFAFEHIVAFTSKSVGSDGILRAVTELARATGPQGIMGGQIVDIASERDAFVDLQTLEWIHIHKTAVLFECATVCGAIIGGASGDEIERIRRFARYLGLLFQVVDDILDVTKSSEDLGKTAGKDLVSDKATYPKLMGLEKAKGFSLELLNKAKEELSCFDPMKAAPLFGLADYMALRQN","length":387,"molWeight":42463,"crc64":"19A281BEB4545BC6","md5":"BEE1674680C4AA4942696BA1E2FAA7D1"},"extraAttributes":{"countByCommentType":{"COFACTOR":1,"PATHWAY":1,"SIMILARITY":1},"uniParcId":"UPI00000AA932"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q8LKJ2","uniProtkbId":"Q8LKJ2_ABIGR","entryAudit":{"firstPublicDate":"2002-10-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2002-10-01","entryVersion":75,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01134.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01134.1"}],"value":"Geranyl diphosphate synthase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01134.1"}],"value":"GPPS2"}}],"comments":[{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005128"}],"value":"Isoprenoid biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006706"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004466"}],"value":"Belongs to the FPP/GGPP synthase family"}],"commentType":"SIMILARITY"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023229"}],"id":"KW-0414","category":"Biological process","name":"Isoprene biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004466"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"12176066","citationType":"journal article","authors":["Burke C.","Croteau R."],"citationCrossReferences":[{"database":"PubMed","id":"12176066"},{"database":"DOI","id":"10.1016/S0003-9861(02)00335-1"}],"title":"Geranyl diphosphate synthase from Abies grandis: cDNA isolation, functional expression, and characterization.","publicationDate":"2002","journal":"Arch. Biochem. Biophys.","firstPage":"130","lastPage":"136","volume":"405"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01134.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF513112","properties":[{"key":"ProteinId","value":"AAN01134.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"Q8LKJ2","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8LKJ2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0004659","properties":[{"key":"GoTerm","value":"F:prenyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008299","properties":[{"key":"GoTerm","value":"P:isoprenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00685","properties":[{"key":"EntryName","value":"Trans_IPPS_HT"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000001","properties":[{"key":"EntryName","value":"Geranylgeranyl diphosphate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR000092","properties":[{"key":"EntryName","value":"Polyprenyl_synt"}]},{"database":"InterPro","id":"IPR033749","properties":[{"key":"EntryName","value":"Polyprenyl_synt_CS"}]},{"database":"InterPro","id":"IPR053378","properties":[{"key":"EntryName","value":"Prenyl_diphosphate_synthase"}]},{"database":"NCBIfam","id":"NF045485","properties":[{"key":"EntryName","value":"FPPsyn"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43281","properties":[{"key":"EntryName","value":"FARNESYL DIPHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43281:SF1","properties":[{"key":"EntryName","value":"FARNESYL DIPHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00348","properties":[{"key":"EntryName","value":"polyprenyl_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01017","properties":[{"key":"EntryName","value":"Polyprenyl_Transferase_Like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00723","properties":[{"key":"EntryName","value":"POLYPRENYL_SYNTHASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00444","properties":[{"key":"EntryName","value":"POLYPRENYL_SYNTHASE_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAYSAMATMGYNGMAASCHTLHPTSPLKPFHGASTSLEAFNGEHMGLLRGYSKRKLSSYKNPASRSSNATVAQLLNPPQKGKKAVEFDFNKYMDSKAMTVNEALNKAIPLRYPQKIYESMRYSLLAGGKRVRPVLCIAACELVGGTEELAIPTACAIEMIHTMSLMHDDLPCIDNDDLRRGKPTNHKIFGEDTAVTAGNALHSYAFEHIAVSTSKTVGADRILRMVSELGRATGSEGVMGGQMVDIASEGDPSIDLQTLEWIHIHKTAMLLECSVVCGAIIGGASEIVIERARRYARCVGLLFQVVDDILDVTKSSDELGKTAGKDLISDKATYPKLMGLEKAKEFSDELLNRAKGELSCFDPVKAAPLLGLADYVAFRQN","length":381,"molWeight":41325,"crc64":"383AAE8C7DF5CF0A","md5":"5FCDD0A8E98CD7FA2F4F2AB3AFDC19B5"},"extraAttributes":{"countByCommentType":{"COFACTOR":1,"PATHWAY":1,"SIMILARITY":1},"uniParcId":"UPI00000A12F8"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q8LKJ3","uniProtkbId":"Q8LKJ3_ABIGR","entryAudit":{"firstPublicDate":"2002-10-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2002-10-01","entryVersion":78,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01133.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01133.1"}],"value":"Geranyl diphosphate synthase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01133.1"}],"value":"GPPS1"}}],"comments":[{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005128"}],"value":"Isoprenoid biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006706"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004466"}],"value":"Belongs to the FPP/GGPP synthase family"}],"commentType":"SIMILARITY"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023229"}],"id":"KW-0414","category":"Biological process","name":"Isoprene biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004466"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"12176066","citationType":"journal article","authors":["Burke C.","Croteau R."],"citationCrossReferences":[{"database":"PubMed","id":"12176066"},{"database":"DOI","id":"10.1016/S0003-9861(02)00335-1"}],"title":"Geranyl diphosphate synthase from Abies grandis: cDNA isolation, functional expression, and characterization.","publicationDate":"2002","journal":"Arch. Biochem. Biophys.","firstPage":"130","lastPage":"136","volume":"405"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAN01133.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF513111","properties":[{"key":"ProteinId","value":"AAN01133.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"Q8LKJ3","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0004659","properties":[{"key":"GoTerm","value":"F:prenyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008299","properties":[{"key":"GoTerm","value":"P:isoprenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00685","properties":[{"key":"EntryName","value":"Trans_IPPS_HT"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000001","properties":[{"key":"EntryName","value":"Geranylgeranyl diphosphate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR000092","properties":[{"key":"EntryName","value":"Polyprenyl_synt"}]},{"database":"InterPro","id":"IPR033749","properties":[{"key":"EntryName","value":"Polyprenyl_synt_CS"}]},{"database":"InterPro","id":"IPR053378","properties":[{"key":"EntryName","value":"Prenyl_diphosphate_synthase"}]},{"database":"NCBIfam","id":"NF045485","properties":[{"key":"EntryName","value":"FPPsyn"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43281","properties":[{"key":"EntryName","value":"FARNESYL DIPHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43281:SF1","properties":[{"key":"EntryName","value":"FARNESYL DIPHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00348","properties":[{"key":"EntryName","value":"polyprenyl_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01017","properties":[{"key":"EntryName","value":"Polyprenyl_Transferase_Like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00723","properties":[{"key":"EntryName","value":"POLYPRENYL_SYNTHASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00444","properties":[{"key":"EntryName","value":"POLYPRENYL_SYNTHASE_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAYSCMAASCHGLHFMNIASQECNLKRGIIPSKRLHGISSSLWASNGFQGHLERDLSAYRHLVSSSRCLNTIAMLSNLSEQAKEKATEFDFKEYLHSKAISVNEALERAVPLRYPEKIHEAMRYSLLAGGKRIRPILTIAACELVGGSEELAMPTACAMEMIHTMSLIHDDLPSMDNDDLRRGKLTNHKVFGEGTAVLAGDALLSFAFEHIAVSTRKTVASHRVLRVVSELGKAIGSQGVAGGQVADITSEGDPSVGLETLEWIHIHKTAVLLECAVVSGAIIGGASENEIERTGRYARCVGLLFQVVDDILDVTRSSEELGKTAGKDLVSDKATYPKLMGLEKAKEFADELLDRAKEELSCFNPAKAAPLLGLADYIALRQN","length":383,"molWeight":41593,"crc64":"BD0E9E217B250E44","md5":"FC0FA186A90D48619EB66E86F34841B9"},"extraAttributes":{"countByCommentType":{"COFACTOR":1,"PATHWAY":1,"SIMILARITY":1},"uniParcId":"UPI00000A5AD2"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q8W1R9","uniProtkbId":"Q8W1R9_ABIGR","entryAudit":{"firstPublicDate":"2002-03-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2002-03-01","entryVersion":77,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Abies grandis","commonName":"Grand fir","synonyms":["Pinus grandis"],"taxonId":46611,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL17614.2"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Abies"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL17614.2"}],"value":"Geranylgeranyl diphosphate synthase"}}]},"comments":[{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005128"}],"value":"Isoprenoid biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006706"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004466"}],"value":"Belongs to the FPP/GGPP synthase family"}],"commentType":"SIMILARITY"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023229"}],"id":"KW-0414","category":"Biological process","name":"Isoprene biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004466"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-SDSG5V6A38BV","citationType":"submission","authors":["Burke C.C.","Croteau R.B."],"publicationDate":"DEC-2001","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL17614.2"}]},{"referenceNumber":2,"citation":{"id":"11733504","citationType":"journal article","authors":["Burke C.","Croteau R."],"citationCrossReferences":[{"database":"PubMed","id":"11733504"},{"database":"DOI","id":"10.1074/jbc.M105900200"}],"title":"Interaction with the small subunit of geranyl diphosphate synthase modifies the chain length specificity of geranylgeranyl diphosphate synthase to produce geranyl diphosphate.","publicationDate":"2002","journal":"J. Biol. Chem.","firstPage":"3141","lastPage":"3149","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL17614.2"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF425235","properties":[{"key":"ProteinId","value":"AAL17614.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"Q8W1R9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8W1R9","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.5.1.29","properties":[{"key":"OrganismId","value":"2"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0004659","properties":[{"key":"GoTerm","value":"F:prenyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008299","properties":[{"key":"GoTerm","value":"P:isoprenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00685","properties":[{"key":"EntryName","value":"Trans_IPPS_HT"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000001","properties":[{"key":"EntryName","value":"Geranylgeranyl diphosphate synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR000092","properties":[{"key":"EntryName","value":"Polyprenyl_synt"}]},{"database":"InterPro","id":"IPR033749","properties":[{"key":"EntryName","value":"Polyprenyl_synt_CS"}]},{"database":"InterPro","id":"IPR053378","properties":[{"key":"EntryName","value":"Prenyl_diphosphate_synthase"}]},{"database":"NCBIfam","id":"NF045485","properties":[{"key":"EntryName","value":"FPPsyn"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43281","properties":[{"key":"EntryName","value":"FARNESYL DIPHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43281:SF1","properties":[{"key":"EntryName","value":"FARNESYL DIPHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00348","properties":[{"key":"EntryName","value":"polyprenyl_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01017","properties":[{"key":"EntryName","value":"Polyprenyl_Transferase_Like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00723","properties":[{"key":"EntryName","value":"POLYPRENYL_SYNTHASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00444","properties":[{"key":"EntryName","value":"POLYPRENYL_SYNTHASE_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAYSGMATSYHGLHFMNIATQECNLKRLSIPSRRFHGVSPSLWASNGFQGHLKRELSANSFLVSSSRYSNTIAKFTNLPEKVKEKVIEFDFKEYLRSKAMAVNEALDRAVPLRYPERIHEAMRYSLLAGGKRVRPVLCISACELVGGTEEVAMPTACAMEMIHTMSLIHDDLPCMDNDDFRRGKPTNHKVFGEGTAILAGDALLSFAFEHIAVSTSKSVGTDRILRVVSELGRTIGSQGLVGGQVADITSEGDASVDLDTLEWIHIHKTAVLLECSVMCGAIISGASDNEIERIQRYARSVGLLFQVVDDILDVTKSSKELGKTAGKDLISDKATYPKLMGLEKAKQFASDLLIRAKEDLSCFDPMKAAPLLGLADYIAFRQN","length":383,"molWeight":42069,"crc64":"EA8D3A015B51CD31","md5":"908FD7A98218F577A10F305FB8CF8006"},"extraAttributes":{"countByCommentType":{"COFACTOR":1,"PATHWAY":1,"SIMILARITY":1},"uniParcId":"UPI00000A51FB"}}]}