{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O67987","uniProtkbId":"TFDC_RHOOP","entryAudit":{"firstPublicDate":"1998-12-15","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"1998-08-01","entryVersion":103,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Rhodococcus opacus","commonName":"Nocardia opaca","taxonId":37919,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Mycobacteriales","Nocardiaceae","Rhodococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15060064"}],"value":"4-chlorocatechol 1,2-dioxygenase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23261399"}],"value":"4-CCD"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}],"value":"1.13.11.-"}]}},"genes":[{"geneName":{"value":"clcA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}],"value":"Chlorocatechol 1,2-dioxygenase that catalyzes a key step in the degradation of chlorinated benzenes, a process that proceeds via chlorocatechol intermediates (PubMed:23261399, PubMed:7813460). Displays broad substrate specificity (PubMed:23261399, PubMed:7813460). Preferentially cleaves 4-chlorocatechol, 4-methylcatechol, 4-fluorocatechol and 3,5-dichlorocatechol, but shows lower activity on catechol, 3-chlorocatechol, 3-methylcatechol, 3-fluorocatechol, 3,6-dichlorocatechol, 4,5-dichlorocatechol, 3-methoxycatechol, pyrogallol (benzene-1,2,3-triol) and hydroxyquinol (benzene-1,2,4-triol), in vitro (PubMed:23261399, PubMed:7813460)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-chlorocatechol + O2 = 3-chloro-cis,cis-muconate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:48576"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:17589"},{"database":"ChEBI","id":"CHEBI:27772"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3,5-dichlorocatechol + O2 = (2E,4E)-2,4-dichloromuconate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:48572"},{"database":"ChEBI","id":"CHEBI:11438"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15788"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"benzene-1,2,4-triol + O2 = 3-hydroxy-cis,cis-muconate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19441"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16971"},{"database":"ChEBI","id":"CHEBI:58139"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"1,2,3-trihydroxybenzene + O2 = (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19673"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16164"},{"database":"ChEBI","id":"CHEBI:28080"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"catechol + O2 = cis,cis-muconate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23852"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:18135"},{"database":"ChEBI","id":"CHEBI:32379"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-chlorocatechol + O2 = (2E,4Z)-2-chloromuconate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:48568"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:19504"},{"database":"ChEBI","id":"CHEBI:27715"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-methylcatechol + O2 = 2-hydroxy-6-oxo-2,4-heptadienoate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:48996"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:18404"},{"database":"ChEBI","id":"CHEBI:90887"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3,6-dichlorocatechol + O2 = (2E,4E)-2,5-dichloromuconate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:35055"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:19375"},{"database":"ChEBI","id":"CHEBI:77767"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Fe(3+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15060064"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29034"}},{"name":"Mn(3+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29041"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15060064"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}],"value":"Binds 1 Fe(3+) ion per subunit (PubMed:15060064, PubMed:23261399). Binds 1 Fe(3+) ion and 1 Mn(3+) ion per homodimer (PubMed:7813460)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}],"value":"Competitively inhibited by protocatechuate (3,4-dihydroxybenzoate), 3,4,5-trichlorocatechol and 3,4,5,6-tetrachlorocatechol"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":28.0,"unit":"uM","substrate":"pyrogallol (at pH 7.2 and 25 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"}]},{"constant":71.0,"unit":"uM","substrate":"hydroxyquinol (at pH 7.2 and 25 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"}]},{"constant":23.3,"unit":"uM","substrate":"catechol (at 25 degrees 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Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]},{"constant":6.6,"unit":"mM","substrate":"O(2) with catechol as cosubstrate (at pH 7.2 and 25 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]},{"constant":6.3,"unit":"mM","substrate":"O(2) with 3,5-dichlorocatechol as cosubstrate (at pH 7.2 and 25 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]},{"constant":3.5,"unit":"mM","substrate":"O(2) with 4-chlorocatechol as cosubstrate (at pH 7.2 and 25 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}],"value":"kcat is 400.4 min(-1) with pyrogallol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:23261399). kcat is 113.6 min(-1) with hydroxyquinol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:23261399). kcat is 268 min(-1) with catechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 36 min(-1) with 3-chlorocatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 253 min(-1) with 4-chlorocatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 49 min(-1) with 3,5-dichlorocatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 33 min(-1) with 3-fluorocatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 569 min(-1) with 4-fluorocatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 557 min(-1) with 3-methylcatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 648 min(-1) with 4-methylcatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 51 min(-1) with 3-methoxycatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 14 min(-1) with 3,6-dichlorocatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460). kcat is 14 min(-1) with 4,5-dichlorocatechol as substrate (at pH 7.2 and 25 degrees Celsius) (PubMed:7813460)."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"15060064"}],"value":"Aromatic compound metabolism; 3-chlorocatechol degradation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15060064"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23261399"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7813460"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15060064"}],"value":"Contains a helical zipper domain, consisting of five N-terminal helices from each subunit, which forms the molecular dimer axis"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Chlorocatechol 1,2-dioxygenases are crucial for bioremediation, breaking down toxic industrial chemicals like polychlorinated biphenyls and herbicides, which are recalcitrant environmental pollutants"}],"commentType":"BIOTECHNOLOGY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15060064"}],"value":"The carboxyl group of benzoates and the hydroxyl group of catechol coordinate to the Fe(3+) center via different modes without altering the orientation of the aromatic ring"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the intradiol ring-cleavage dioxygenase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator 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