{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P24674","uniProtkbId":"RBL_PICPU","entryAudit":{"firstPublicDate":"1992-03-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1992-03-01","entryVersion":114,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Picea pungens","commonName":"Colorado spruce","synonyms":["Picea parryana"],"taxonId":3331,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Picea"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"Ribulose bisphosphate carboxylase large chain"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"RuBisCO large subunit"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"4.1.1.39"}]},"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"rbcL"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 (2R)-3-phosphoglycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO2 + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23124"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57870"},{"database":"ChEBI","id":"CHEBI:58272"}],"ecNumber":"4.1.1.39","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-ribulose 1,5-bisphosphate + O2 = 2-phosphoglycolate + (2R)-3-phosphoglycerate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:36631"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57870"},{"database":"ChEBI","id":"CHEBI:58033"},{"database":"ChEBI","id":"CHEBI:58272"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"Binds 1 Mg(2+) ion per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"value":"Belongs to the RuBisCO large chain family. Type I subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Propeptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"featureId":"PRO_0000031351"},{"type":"Chain","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":475,"modifier":"EXACT"}},"description":"Ribulose bisphosphate carboxylase large chain","featureId":"PRO_0000031352"},{"type":"Active site","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}]},{"type":"Active site","location":{"start":{"value":294,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}]},{"type":"Binding site","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"in homodimeric partner","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":173,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"via carbamate group","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":203,"modifier":"EXACT"},"end":{"value":203,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":204,"modifier":"EXACT"},"end":{"value":204,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":295,"modifier":"EXACT"},"end":{"value":295,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":327,"modifier":"EXACT"},"end":{"value":327,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":379,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}],"ligand":{"name":"substrate"}},{"type":"Site","location":{"start":{"value":334,"modifier":"EXACT"},"end":{"value":334,"modifier":"EXACT"}},"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}]},{"type":"Modified residue","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":3,"modifier":"EXACT"}},"description":"N-acetylproline","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}]},{"type":"Modified residue","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":14,"modifier":"EXACT"}},"description":"N6,N6,N6-trimethyllysine","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}]},{"type":"Modified residue","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"N6-carboxylysine","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}]},{"type":"Disulfide bond","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"Interchain; in linked form","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01338"}]}],"geneLocations":[{"geneEncodingType":"Chloroplast"}],"keywords":[{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0113","category":"Biological process","name":"Calvin cycle"},{"id":"KW-0120","category":"Biological process","name":"Carbon dioxide fixation"},{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0488","category":"PTM","name":"Methylation"},{"id":"KW-0503","category":"Molecular function","name":"Monooxygenase"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"id":"KW-0601","category":"Biological process","name":"Photorespiration"},{"id":"KW-0602","category":"Biological process","name":"Photosynthesis"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-6L8A2LU5KV8A4","citationType":"submission","authors":["Doerksen A.H.","Strauss S.","Price R."],"publicationDate":"MAR-1991","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X58136","properties":[{"key":"ProteinId","value":"CAA41144.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S15494","properties":[{"key":"EntryName","value":"RKJQLC"}]},{"database":"RefSeq","id":"YP_010526797.1","properties":[{"key":"NucleotideSequenceId","value":"NC_067714.1"}]},{"database":"AlphaFoldDB","id":"P24674","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P24674","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"76329133","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016984","properties":[{"key":"GoTerm","value":"F:ribulose-bisphosphate carboxylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019253","properties":[{"key":"GoTerm","value":"P:reductive pentose-phosphate cycle"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd08212","properties":[{"key":"EntryName","value":"RuBisCO_large_I"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.110:FF:000001","properties":[{"key":"EntryName","value":"Ribulose bisphosphate carboxylase large chain"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.150:FF:000001","properties":[{"key":"EntryName","value":"Ribulose bisphosphate carboxylase large chain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.110","properties":[{"key":"EntryName","value":"Ribulose bisphosphate carboxylase, large subunit, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.150","properties":[{"key":"EntryName","value":"RuBisCO large subunit, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01338","properties":[{"key":"EntryName","value":"RuBisCO_L_type1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR033966","properties":[{"key":"EntryName","value":"RuBisCO"}]},{"database":"InterPro","id":"IPR020878","properties":[{"key":"EntryName","value":"RuBisCo_large_chain_AS"}]},{"database":"InterPro","id":"IPR000685","properties":[{"key":"EntryName","value":"RuBisCO_lsu_C"}]},{"database":"InterPro","id":"IPR036376","properties":[{"key":"EntryName","value":"RuBisCO_lsu_C_sf"}]},{"database":"InterPro","id":"IPR017443","properties":[{"key":"EntryName","value":"RuBisCO_lsu_fd_N"}]},{"database":"InterPro","id":"IPR036422","properties":[{"key":"EntryName","value":"RuBisCO_lsu_N_sf"}]},{"database":"InterPro","id":"IPR020888","properties":[{"key":"EntryName","value":"RuBisCO_lsuI"}]},{"database":"NCBIfam","id":"NF003252","properties":[{"key":"EntryName","value":"PRK04208.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42704","properties":[{"key":"EntryName","value":"RIBULOSE BISPHOSPHATE CARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42704:SF15","properties":[{"key":"EntryName","value":"RIBULOSE BISPHOSPHATE CARBOXYLASE LARGE CHAIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00016","properties":[{"key":"EntryName","value":"RuBisCO_large"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02788","properties":[{"key":"EntryName","value":"RuBisCO_large_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01052","properties":[{"key":"EntryName","value":"RuBisCO"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00014","properties":[{"key":"EntryName","value":"RuBisCO"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG00301","properties":[{"key":"EntryName","value":"RuBisCO-like_proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51649","properties":[{"key":"EntryName","value":"RuBisCo, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54966","properties":[{"key":"EntryName","value":"RuBisCO, large subunit, small (N-terminal) domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00157","properties":[{"key":"EntryName","value":"RUBISCO_LARGE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPKTETKASVGFKAGVKDYRLTYYTPEYQTKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYDIEPVAGEETQFIAFVAYPLDLFEEGSVTNLFTSIVGNVFGFKALRALRLEDLRIPPAYSKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFVFCAEAIYKAQAETGEIKGHYLNATAGTCEEMMKRAVFARELGVPIVMHDYLTGGFTANTSLAHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRMSGGDHIHGGTVVGKLEGERDITLGFVDLLRDDFIEKDRSRGIYFTQDWVSMPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQARNEGRDLAREGNEVIREASKWSPELAAACEVWKEIKFEFEAVDTI","length":475,"molWeight":52592,"crc64":"F0A1757153FC3BB5","md5":"8CE4029C04E5E32F89B3E352F4DAD82A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"PTM":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Propeptide":1,"Chain":1,"Active site":2,"Binding site":9,"Site":1,"Modified residue":3,"Disulfide bond":1},"uniParcId":"UPI000013331B"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q7HEK3","uniProtkbId":"MATK_PICPU","entryAudit":{"firstPublicDate":"2005-06-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":53,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Picea pungens","commonName":"Colorado spruce","synonyms":["Picea parryana"],"taxonId":3331,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Picea"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01390"}],"value":"Maturase K"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01390"}],"value":"Intron maturase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01390"}],"value":"matK"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01390"}],"value":"Usually encoded in the trnK tRNA gene intron. Probably assists in splicing its own and other chloroplast group II introns"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01390"}],"value":"Belongs to the intron maturase 2 family. MatK subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":515,"modifier":"EXACT"}},"description":"Maturase K","featureId":"PRO_0000143599"}],"geneLocations":[{"geneEncodingType":"Chloroplast"}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0507","category":"Biological process","name":"mRNA processing"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0819","category":"Biological process","name":"tRNA processing"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-240NP9676UTP6","citationType":"submission","authors":["Germano J.","Thorner A.R.","Klein A.S."],"title":"Phylogenetic analysis of Picea species based on chloroplast and mitochondrial gene sequences.","publicationDate":"MAY-2001","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY035195","properties":[{"key":"ProteinId","value":"AAK61803.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"YP_010526847.1","properties":[{"key":"NucleotideSequenceId","value":"NC_067714.1"}]},{"database":"AlphaFoldDB","id":"Q7HEK3","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"76329217","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0006397","properties":[{"key":"GoTerm","value":"P:mRNA processing"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008380","properties":[{"key":"GoTerm","value":"P:RNA splicing"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_01390","properties":[{"key":"EntryName","value":"MatK"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR024937","properties":[{"key":"EntryName","value":"Domain_X"}]},{"database":"InterPro","id":"IPR002866","properties":[{"key":"EntryName","value":"Maturase_MatK"}]},{"database":"InterPro","id":"IPR024942","properties":[{"key":"EntryName","value":"Maturase_MatK_N"}]},{"database":"PANTHER","id":"PTHR34811","properties":[{"key":"EntryName","value":"MATURASE K"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR34811:SF1","properties":[{"key":"EntryName","value":"MATURASE K"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01348","properties":[{"key":"EntryName","value":"Intron_maturas2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01824","properties":[{"key":"EntryName","value":"MatK_N"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDEFHRYGKEDSSWQQCFLYPLFFQEDLYAISHDHYLDGSSSSEPMEHLSSNDQFSFLTVKRLIGQIRQQNHSIVLFVNCDPNPLVDRKKSSYSESVLEGLTLVLEVPFSIRSKYSVEGMNEWKSFRSIHSIFPFLEDKFPHSNYVSDTRIPYSIHPEILVRTFRRWIGDAPSLHPLRSILYEYRNSSESLQRSIIVVPKVNTRFFLFLWNNYVYECESILVSLLKRSSHSRSLSHGSFPQRTHFHRKIKNIFLFSRRNSFQSIWSLKDPNIHYVRYGERSIIAIKGTHLLVKKYRYYLPIFRQCYFHLWNEPYRVCSHQLSKNCSSSLGYFLRVRMKPLLVKTKMLDELFIADLITDEFDPIVPIVPIIGLLSREKFCDISGRPISKLSWTSLTDDDILDRFDRIWRNLFHYYSGSFGRDGLYRIKYILSLSCAKTLACKHKSTIRVVRKELGPELFKKSFSKERELDSPPFSSKAAARSQRERIWHSDIPQINPLAHSWQKIQDLKIENLFDQ","length":515,"molWeight":60892,"crc64":"5BA434B7681F17F5","md5":"56619C62CB36691D02DAA16260BB8102"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI0000091373"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A2CHC0","uniProtkbId":"A2CHC0_PICPU","entryAudit":{"firstPublicDate":"2007-02-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-02-20","entryVersion":66,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Picea pungens","commonName":"Colorado spruce","synonyms":["Picea parryana"],"taxonId":3331,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Picea"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021253"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"value":"Cytochrome b6-f complex subunit 8"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031459"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"value":"Cytochrome b6-f complex subunit PetN"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031982"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"value":"Cytochrome b6-f complex subunit VIII"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}],"value":"petN"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003068"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"value":"Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00025834"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"value":"The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004167"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004167"}],"value":"Single-pass membrane protein","id":"SL-9904"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"value":"Plastid, chloroplast thylakoid membrane","id":"SL-0058"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010969"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"value":"Belongs to the PetN family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"id":"KW-0602","category":"Biological process","name":"Photosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"id":"KW-0793","category":"Cellular component","name":"Thylakoid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00395"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-AOEHB60PMJ7M","citationType":"submission","authors":["Ran J.-H.","Wei X.-X.","Wang X.-Q."],"publicationDate":"APR-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}],"value":"32","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}],"value":"Needle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}]},{"referenceNumber":2,"citation":{"id":"16839785","citationType":"journal article","authors":["Ran J.H.","Wei X.X.","Wang X.Q."],"citationCrossReferences":[{"database":"PubMed","id":"16839785"},{"database":"DOI","id":"10.1016/j.ympev.2006.05.039"}],"title":"Molecular phylogeny and biogeography of Picea (Pinaceae): implications for phylogeographical studies using cytoplasmic haplotypes.","publicationDate":"2006","journal":"Mol. Phylogenet. Evol.","firstPage":"405","lastPage":"419","volume":"41"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}],"value":"32","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}],"value":"Needle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59502.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DQ010583","properties":[{"key":"ProteinId","value":"AAY59502.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"YP_010526802.1","properties":[{"key":"NucleotideSequenceId","value":"NC_067714.1"}]},{"database":"AlphaFoldDB","id":"A2CHC0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A2CHC0","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"76329146","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009535","properties":[{"key":"GoTerm","value":"C:chloroplast thylakoid membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0009512","properties":[{"key":"GoTerm","value":"C:cytochrome b6f complex"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0017004","properties":[{"key":"GoTerm","value":"P:cytochrome complex assembly"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_00395","properties":[{"key":"EntryName","value":"Cytb6_f_PetN"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036143","properties":[{"key":"EntryName","value":"Cytochr_b6-f_cplx_su8_sf"}]},{"database":"InterPro","id":"IPR005497","properties":[{"key":"EntryName","value":"Cytochrome_b6-f_cplx_su8"}]},{"database":"Pfam","id":"PF03742","properties":[{"key":"EntryName","value":"PetN"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103451","properties":[{"key":"EntryName","value":"PetN subunit of the cytochrome b6f complex"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDIVGITWAALMVVFTFSLSLVVWGRSGL","length":29,"molWeight":3170,"crc64":"3B0215516534DA61","md5":"6F2698966B091CDA59924AD3277EFD03"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":1},"uniParcId":"UPI00001316CE"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A2CHC1","uniProtkbId":"A2CHC1_PICPU","entryAudit":{"firstPublicDate":"2007-02-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-02-20","entryVersion":48,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Picea pungens","commonName":"Colorado spruce","synonyms":["Picea parryana"],"taxonId":3331,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Picea"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"value":"Photosystem II reaction center protein M"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"value":"PSII-M"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}],"value":"psbM"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"value":"One of the components of the core complex of photosystem II (PSII). PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. This subunit is found at the monomer-monomer interface"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"value":"PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Psb30/Ycf12, at least 3 peripheral proteins of the oxygen-evolving complex and a large number of cofactors. It forms dimeric complexes"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004167"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004167"}],"value":"Single-pass membrane protein","id":"SL-9904"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"value":"Plastid, chloroplast thylakoid membrane","id":"SL-0058"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"value":"Belongs to the PsbM family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022531"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"id":"KW-0602","category":"Biological process","name":"Photosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023276"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"id":"KW-0604","category":"Cellular component","name":"Photosystem II"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022469"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"id":"KW-0674","category":"Cellular component","name":"Reaction center"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"id":"KW-0793","category":"Cellular component","name":"Thylakoid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00438"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-AOEHB60PMJ7M","citationType":"submission","authors":["Ran J.-H.","Wei X.-X.","Wang X.-Q."],"publicationDate":"APR-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}],"value":"32","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}],"value":"Needle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}]},{"referenceNumber":2,"citation":{"id":"16839785","citationType":"journal article","authors":["Ran J.H.","Wei X.X.","Wang X.Q."],"citationCrossReferences":[{"database":"PubMed","id":"16839785"},{"database":"DOI","id":"10.1016/j.ympev.2006.05.039"}],"title":"Molecular phylogeny and biogeography of Picea (Pinaceae): implications for phylogeographical studies using cytoplasmic haplotypes.","publicationDate":"2006","journal":"Mol. Phylogenet. Evol.","firstPage":"405","lastPage":"419","volume":"41"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}],"value":"32","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}],"value":"Needle","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY59503.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DQ010583","properties":[{"key":"ProteinId","value":"AAY59503.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"YP_010526801.1","properties":[{"key":"NucleotideSequenceId","value":"NC_067714.1"}]},{"database":"AlphaFoldDB","id":"A2CHC1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A2CHC1","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"76329145","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009535","properties":[{"key":"GoTerm","value":"C:chloroplast thylakoid membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0009523","properties":[{"key":"GoTerm","value":"C:photosystem II"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0019684","properties":[{"key":"GoTerm","value":"P:photosynthesis, light reaction"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"HAMAP","id":"MF_00438","properties":[{"key":"EntryName","value":"PSII_PsbM"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007826","properties":[{"key":"EntryName","value":"PSII_PsbM"}]},{"database":"InterPro","id":"IPR037269","properties":[{"key":"EntryName","value":"PSII_PsbM_sf"}]},{"database":"NCBIfam","id":"TIGR03038","properties":[{"key":"EntryName","value":"PS_II_psbM"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35774","properties":[{"key":"EntryName","value":"PHOTOSYSTEM II REACTION CENTER PROTEIN M"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35774:SF1","properties":[{"key":"EntryName","value":"PHOTOSYSTEM II REACTION CENTER PROTEIN M"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05151","properties":[{"key":"EntryName","value":"PsbM"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF161033","properties":[{"key":"EntryName","value":"Photosystem II reaction center protein M, PsbM"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEVNTLAFIAVLLFLAISTAFLFIPYVKTASASSGNN","length":37,"molWeight":3936,"crc64":"7B98C11094AEB7DF","md5":"A7832160F51BFA033CE631D3D9345567"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":1},"uniParcId":"UPI0000EE07A9"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"I3QDW6","uniProtkbId":"I3QDW6_PICPU","entryAudit":{"firstPublicDate":"2012-09-05","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2012-09-05","entryVersion":46,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Picea pungens","commonName":"Colorado spruce","synonyms":["Picea parryana"],"taxonId":3331,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFJ73583.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Pinopsida","Pinidae","Conifers I","Pinales","Pinaceae","Picea"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFJ73583.1"}],"value":"2-methyl-3-buten-2-ol synthase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFJ73583.1"}],"value":"Tps-MBO3"}}],"comments":[{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005140"}],"value":"Terpene metabolism; oleoresin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004229"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008762"}],"value":"Belongs to the terpene synthase family. Tpsd subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Terpene synthase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01397"}]},{"type":"Domain","location":{"start":{"value":331,"modifier":"EXACT"},"end":{"value":565,"modifier":"EXACT"}},"description":"Terpene synthase-like metal-binding","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF03936"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022640"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022946"}],"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-CM0NKK1K5PRR5","citationType":"submission","authors":["Gray D.W.","Breneman S.R.","Sharkey T.D."],"title":"MBO evolution.","publicationDate":"MAY-2011","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFJ73583.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"JN039265","properties":[{"key":"ProteinId","value":"AFJ73583.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"I3QDW6","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00924","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010333","properties":[{"key":"GoTerm","value":"F:terpene synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016102","properties":[{"key":"GoTerm","value":"P:diterpenoid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00684","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.50.10.130:FF:000004","properties":[{"key":"EntryName","value":"Carene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.600.10:FF:000005","properties":[{"key":"EntryName","value":"Ent-kaur-16-ene synthase, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.600.10","properties":[{"key":"EntryName","value":"Farnesyl Diphosphate Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.130","properties":[{"key":"EntryName","value":"Terpene synthase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008949","properties":[{"key":"EntryName","value":"Isoprenoid_synthase_dom_sf"}]},{"database":"InterPro","id":"IPR034741","properties":[{"key":"EntryName","value":"Terpene_cyclase-like_1_C"}]},{"database":"InterPro","id":"IPR044814","properties":[{"key":"EntryName","value":"Terpene_cyclase_plant_C1"}]},{"database":"InterPro","id":"IPR001906","properties":[{"key":"EntryName","value":"Terpene_synth_N"}]},{"database":"InterPro","id":"IPR036965","properties":[{"key":"EntryName","value":"Terpene_synth_N_sf"}]},{"database":"InterPro","id":"IPR050148","properties":[{"key":"EntryName","value":"Terpene_synthase-like"}]},{"database":"InterPro","id":"IPR005630","properties":[{"key":"EntryName","value":"Terpene_synthase_metal-bd"}]},{"database":"InterPro","id":"IPR008930","properties":[{"key":"EntryName","value":"Terpenoid_cyclase/PrenylTrfase"}]},{"database":"PANTHER","id":"PTHR31739:SF25","properties":[{"key":"EntryName","value":"(E,E)-GERANYLLINALOOL SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31739","properties":[{"key":"EntryName","value":"ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01397","properties":[{"key":"EntryName","value":"Terpene_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03936","properties":[{"key":"EntryName","value":"Terpene_synth_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00005","properties":[{"key":"EntryName","value":"Isoprenoid_Synthase_Type_I"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01019","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_C_Termi"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01014","properties":[{"key":"EntryName","value":"Terpene_Cyclase_Like_1_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48239","properties":[{"key":"EntryName","value":"Terpenoid cyclases/Protein prenyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48576","properties":[{"key":"EntryName","value":"Terpenoid synthases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALLSVAPLVSTWCVDKPLVGSSEAKALLRKIPTLEMCRLTKSVTPSISMCLTTTVSDDGVQRRIANHHSNLWDDNFIQSLSTPYGATAYHERAQKLIGEVKVIINSILVEDGELITAPNDLLQRLSIVDSIERLGIDRHFKNEIKSALDYVYSYWNEKGIGCGRDSVVNDLNTTALGLRTLRLHGYPVSSDVLEQFKDQNGQFACSAIQTEGEIKKVLNLFRASLIAFPGEKVMEEAEIFSTIYLKEALLKIPVCSFSREIAYVLEYGWHMNLPRLEARNYIDVFGQDAIYLTPNMRTQKILELAKLEFNIFHSLQQKELKHLSRWWKDSVFSQLTFPRHRHVEYYTLASCIDIDPQHSSFRLGFAKIFHLATVLDDIYDTFGMMDELELFTAAVKRWHPSAAEWLPEYMKGVYMMLYETVYEMAREAEKSQGRDTLNYARQALEAYIDSYMKEAKWISSGFLPTFEEYLDNGKVSFGYRIGTLQPILTLGIPFPHHILQEIDFPSRLNDLAGSILRLKGDIHSYQAERSRGEESSGISCYMKDNPESTEEDAVTYINAMVNRLLKELNWELLKPHSNVPITSKKHAFDILRAFYHLYKDRDGFSVTRNEIRNLVMTTVIEPVPL","length":626,"molWeight":71724,"crc64":"0EC9F4712FFC5EBA","md5":"67CC02B78C32268A90FE828C9E408CA7"},"extraAttributes":{"countByCommentType":{"COFACTOR":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2},"uniParcId":"UPI000263A9F2"}}]}