{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HLR3","uniProtkbId":"SCX1_CENBA","entryAudit":{"firstPublicDate":"2020-10-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2020-10-07","entryVersion":12,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Centruroides baergi","commonName":"Scorpion","synonyms":["Centruroides nigrovariatus baergi"],"taxonId":329350,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32479835"}],"value":"Beta-toxin Cb1"}}},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and reduces peak current and shifts the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (PubMed:32479835). Has an inhibitory effect on voltage-gated sodium channel hNav1.6/SCN8A, affecting both the activation and inactivation processes (PubMed:32479835). This toxin is active against mammals and lethal to mice (PubMed:32479835)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"Inhibited by human antibodies scFvs 10FG2 and LR"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32479835"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":7522.7,"molWeightError":1.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"LD(50) is 100 ug/kg by intraperitoneal injection into mice"}],"commentType":"TOXIC DOSE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Beta-toxin Cb1","featureId":"PRO_0000450847"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"LCN-type CS-alpha/beta","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]}],"keywords":[{"id":"KW-0108","category":"Molecular function","name":"Calcium channel impairing toxin"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-1218","category":"Molecular function","name":"Voltage-gated calcium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"32479835","citationType":"journal article","authors":["Gomez-Ramirez I.V.","Riano-Umbarila L.","Olamendi-Portugal T.","Restano-Cassulini R.","Possani L.D.","Becerril B."],"citationCrossReferences":[{"database":"PubMed","id":"32479835"},{"database":"DOI","id":"10.1016/j.toxicon.2020.05.021"}],"title":"Biochemical, electrophysiological and immunological characterization of the venom from Centruroides baergi, a new scorpion species of medical importance in Mexico.","publicationDate":"2020","journal":"Toxicon","firstPage":"10","lastPage":"18","volume":"184"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","ACTIVITY REGULATION","SUBCELLULAR LOCATION","MASS SPECTROMETRY","TOXIC DOSE"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HLR3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HLR3","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0019871","properties":[{"key":"GoTerm","value":"F:sodium channel inhibitor activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0090729","properties":[{"key":"GoTerm","value":"F:toxin activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0006952","properties":[{"key":"GoTerm","value":"P:defense response"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0044493","properties":[{"key":"GoTerm","value":"P:venom-mediated inhibition of voltage-gated sodium channel activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"CDD","id":"cd23106","properties":[{"key":"EntryName","value":"neurotoxins_LC_scorpion"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.30.10:FF:000002","properties":[{"key":"EntryName","value":"Alpha-like toxin BmK-M1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003614","properties":[{"key":"EntryName","value":"Knottins"}]},{"database":"InterPro","id":"IPR044062","properties":[{"key":"EntryName","value":"LCN-type_CS_alpha_beta_dom"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR018218","properties":[{"key":"EntryName","value":"Scorpion_toxinL"}]},{"database":"PRINTS","id":"PR00285","properties":[{"key":"EntryName","value":"SCORPNTOXIN"}]},{"database":"SMART","id":"SM00505","properties":[{"key":"EntryName","value":"Knot1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51863","properties":[{"key":"EntryName","value":"LCN_CSAB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"KEGYIVNHSTGCKYECYKLGDNDYCLRECKAQYGKGAGGYCYAFGCWCTHLYEQAVVWPLPKKTCN","length":66,"molWeight":7530,"crc64":"D8653D5B610BB5C2","md5":"C7BD3AB3781EB04EC8538274400B52F6"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"ACTIVITY REGULATION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"TOXIC DOSE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Disulfide bond":4},"uniParcId":"UPI0015EE38FE"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HLR4","uniProtkbId":"SCX2_CENBA","entryAudit":{"firstPublicDate":"2020-10-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2020-10-07","entryVersion":12,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Centruroides baergi","commonName":"Scorpion","synonyms":["Centruroides nigrovariatus baergi"],"taxonId":329350,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32479835"}],"value":"Beta-toxin Cb2"}}},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and reduces peak current and shifts the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (PubMed:32479835). Has an inhibitory effect on voltage-gated sodium channel hNav1.6/SCN8A, affecting both the activation and inactivation processes (PubMed:32479835). Also reduces the peak current of hNav1.5/SCN5A but does not shift its voltage of activation (PubMed:32479835). This toxin is active against mammals and lethal to mice (PubMed:32479835)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32479835"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":7727.6,"molWeightError":1.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"LD(50) is 75 ug/kg by intraperitoneal injection into mice"}],"commentType":"TOXIC DOSE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Beta-toxin Cb2","featureId":"PRO_0000450848"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"LCN-type CS-alpha/beta","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-0738","category":"Molecular function","name":"Voltage-gated sodium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"32479835","citationType":"journal article","authors":["Gomez-Ramirez I.V.","Riano-Umbarila L.","Olamendi-Portugal T.","Restano-Cassulini R.","Possani L.D.","Becerril B."],"citationCrossReferences":[{"database":"PubMed","id":"32479835"},{"database":"DOI","id":"10.1016/j.toxicon.2020.05.021"}],"title":"Biochemical, electrophysiological and immunological characterization of the venom from Centruroides baergi, a new scorpion species of medical importance in Mexico.","publicationDate":"2020","journal":"Toxicon","firstPage":"10","lastPage":"18","volume":"184"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","MASS SPECTROMETRY","TOXIC DOSE"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HLR4","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HLR4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0019871","properties":[{"key":"GoTerm","value":"F:sodium channel inhibitor activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0090729","properties":[{"key":"GoTerm","value":"F:toxin activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0006952","properties":[{"key":"GoTerm","value":"P:defense response"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0044493","properties":[{"key":"GoTerm","value":"P:venom-mediated inhibition of voltage-gated sodium channel activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"CDD","id":"cd23106","properties":[{"key":"EntryName","value":"neurotoxins_LC_scorpion"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.30.10:FF:000002","properties":[{"key":"EntryName","value":"Alpha-like toxin BmK-M1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003614","properties":[{"key":"EntryName","value":"Knottins"}]},{"database":"InterPro","id":"IPR044062","properties":[{"key":"EntryName","value":"LCN-type_CS_alpha_beta_dom"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR018218","properties":[{"key":"EntryName","value":"Scorpion_toxinL"}]},{"database":"PRINTS","id":"PR00285","properties":[{"key":"EntryName","value":"SCORPNTOXIN"}]},{"database":"SMART","id":"SM00505","properties":[{"key":"EntryName","value":"Knot1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51863","properties":[{"key":"EntryName","value":"LCN_CSAB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"KEGYLVDLHTGCKYTCVGLGDNDYCVRECRLRYYDSAHGYCYAFGCWCTHLYEQAVVWPLPNKRCK","length":66,"molWeight":7736,"crc64":"3620C7E4931B6BC6","md5":"D4DFAF4AFFE10079F6305289E9BC6B23"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"TOXIC DOSE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Disulfide bond":4},"uniParcId":"UPI0015EE3901"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HLR5","uniProtkbId":"SCX3_CENBA","entryAudit":{"firstPublicDate":"2020-10-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2020-10-07","entryVersion":12,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Centruroides baergi","commonName":"Scorpion","synonyms":["Centruroides nigrovariatus baergi"],"taxonId":329350,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32479835"}],"value":"Beta-toxin Cb3"}}},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and reduces peak current and shifts the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (PubMed:32479835). Has an inhibitory effect on voltage-gated sodium channels hNav1.1/SCN1A, hNav1.2/SCN2A, hNav1.4/SCN4A and hNav1.6/SCN8A (PubMed:32479835). Reduces the peak current of hNav1.5/SCN5A but does not shift its voltage of activation (PubMed:32479835). Also affects the inactivation processes of hNav1.1/SCN1A, hNav1.4/SCN4A, hNav1.5/SCN5A and hNav1.6/SCN8A (PubMed:32479835). This toxin is active against mammals and lethal to mice (PubMed:32479835)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32479835"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":7775.7,"molWeightError":1.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32479835"}],"value":"LD(50) is 25 ug/kg by intraperitoneal injection into mice"}],"commentType":"TOXIC DOSE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Beta-toxin Cb3","featureId":"PRO_0000450849"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"LCN-type CS-alpha/beta","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-0738","category":"Molecular function","name":"Voltage-gated sodium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"32479835","citationType":"journal article","authors":["Gomez-Ramirez I.V.","Riano-Umbarila L.","Olamendi-Portugal T.","Restano-Cassulini R.","Possani L.D.","Becerril B."],"citationCrossReferences":[{"database":"PubMed","id":"32479835"},{"database":"DOI","id":"10.1016/j.toxicon.2020.05.021"}],"title":"Biochemical, electrophysiological and immunological characterization of the venom from Centruroides baergi, a new scorpion species of medical importance in Mexico.","publicationDate":"2020","journal":"Toxicon","firstPage":"10","lastPage":"18","volume":"184"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","MASS SPECTROMETRY","TOXIC DOSE"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HLR5","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HLR5","properties":[{"key":"Description","value":"-"}]},{"database":"ABCD","id":"C0HLR5","properties":[{"key":"antibodies","value":"2 sequenced antibodies"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0019871","properties":[{"key":"GoTerm","value":"F:sodium channel inhibitor activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0090729","properties":[{"key":"GoTerm","value":"F:toxin activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0006952","properties":[{"key":"GoTerm","value":"P:defense response"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"GO","id":"GO:0044493","properties":[{"key":"GoTerm","value":"P:venom-mediated inhibition of voltage-gated sodium channel activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32479835"}]},{"database":"CDD","id":"cd23106","properties":[{"key":"EntryName","value":"neurotoxins_LC_scorpion"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.30.10:FF:000002","properties":[{"key":"EntryName","value":"Alpha-like toxin BmK-M1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003614","properties":[{"key":"EntryName","value":"Knottins"}]},{"database":"InterPro","id":"IPR044062","properties":[{"key":"EntryName","value":"LCN-type_CS_alpha_beta_dom"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR018218","properties":[{"key":"EntryName","value":"Scorpion_toxinL"}]},{"database":"PRINTS","id":"PR00285","properties":[{"key":"EntryName","value":"SCORPNTOXIN"}]},{"database":"SMART","id":"SM00505","properties":[{"key":"EntryName","value":"Knot1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51863","properties":[{"key":"EntryName","value":"LCN_CSAB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"KEGYIVNYYDGCKYPCVKLGDNDYCLRECRLRYYKSAGGYCYAFACWCTHLYEQAVVWPLPNKTCL","length":66,"molWeight":7783,"crc64":"A8C7634CCC83E423","md5":"2F36C7C0274935F719AEBE87A7818F66"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"TOXIC DOSE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Disulfide bond":4},"uniParcId":"UPI0015EE38F7"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A4P8W1Z8","uniProtkbId":"A0A4P8W1Z8_CENBA","entryAudit":{"firstPublicDate":"2019-07-31","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2019-07-31","entryVersion":24,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Centruroides baergi","commonName":"Scorpion","synonyms":["Centruroides nigrovariatus baergi"],"taxonId":329350,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QCS34915.1"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00015947"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"value":"Cytochrome c oxidase subunit 1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"value":"7.1.1.9"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QCS34915.1"}],"value":"COI"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"value":"Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11436"},{"database":"Rhea","id":"RHEA-COMP:10350"},{"database":"Rhea","id":"RHEA-COMP:14399"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:29033"},{"database":"ChEBI","id":"CHEBI:29034"}],"ecNumber":"7.1.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049512"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:11437"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049512"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"heme","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001971"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:30413"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004673"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"value":"Energy metabolism; oxidative phosphorylation"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004141"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004141"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009578"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"value":"Belongs to the heme-copper respiratory oxidase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":214,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":275,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":287,"modifier":"EXACT"},"end":{"value":309,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":321,"modifier":"EXACT"},"end":{"value":343,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"Cytochrome oxidase subunit I profile","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50855"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QCS34915.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":359,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QCS34915.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QCS34915.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0186","category":"Ligand","name":"Copper"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0349","category":"Ligand","name":"Heme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QCS34915.1"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000369"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"30837519","citationType":"journal article","authors":["Esposito L.A.","Prendini L."],"citationCrossReferences":[{"database":"PubMed","id":"30837519"}],"title":"Island Ancestors and New World Biogeography: A Case Study from the Scorpions (Buthidae: Centruroidinae).","publicationDate":"2019","journal":"Sci. Rep.","firstPage":"0","lastPage":"3500","volume":"9"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QCS34915.1"}],"value":"2068","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QCS34915.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"MK479162","properties":[{"key":"ProteinId","value":"QCS34915.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A4P8W1Z8","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00705","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0004129","properties":[{"key":"GoTerm","value":"F:cytochrome-c oxidase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0020037","properties":[{"key":"GoTerm","value":"F:heme binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0015990","properties":[{"key":"GoTerm","value":"P:electron transport coupled proton transport"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006123","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, cytochrome c to oxygen"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"Gene3D","id":"1.20.210.10","properties":[{"key":"EntryName","value":"Cytochrome c oxidase-like, subunit I domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR023616","properties":[{"key":"EntryName","value":"Cyt_c_oxase-like_su1_dom"}]},{"database":"InterPro","id":"IPR036927","properties":[{"key":"EntryName","value":"Cyt_c_oxase-like_su1_sf"}]},{"database":"InterPro","id":"IPR000883","properties":[{"key":"EntryName","value":"Cyt_C_Oxase_1"}]},{"database":"InterPro","id":"IPR023615","properties":[{"key":"EntryName","value":"Cyt_c_Oxase_su1_BS"}]},{"database":"PANTHER","id":"PTHR10422","properties":[{"key":"EntryName","value":"CYTOCHROME C OXIDASE SUBUNIT 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10422:SF18","properties":[{"key":"EntryName","value":"CYTOCHROME C OXIDASE SUBUNIT 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00115","properties":[{"key":"EntryName","value":"COX1"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01165","properties":[{"key":"EntryName","value":"CYCOXIDASEI"}]},{"database":"SUPFAM","id":"SSF81442","properties":[{"key":"EntryName","value":"Cytochrome c oxidase subunit I-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50855","properties":[{"key":"EntryName","value":"COX1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00077","properties":[{"key":"EntryName","value":"COX1_CUB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"TMYLVLGIWASMVGTALSLIVRGEIGMPGSLIGDDQVYNVVVTAHAFVMIFFMVMPIMIGGFGNWLVPLMLGAPDMAFPRMNNMSFWLLPPAFFLLLSSAALESGAGTGWTVYPPLSSSLAHVGGSVDLTIFSLHLAGVSSILGAINFITTIINMRSSGMGLDRLPLFVWSVMVTAVLLLLSLPVLAGAITMLLTDRNFNTSFFDPAGGGDPVLYQHLFWFFGHPEVYILILPGFGMISHIVSHHMGKKEPFGSLGMIYAMVAIGGLGFIVWAHHMFTVGMDVDTRAYFTAATMIIAVPTGIKIFSWLATLHGSYFVFSSAMLWALGFIFLFTVGGLTGVILANSSLDIVLHDTYYVVA","length":359,"molWeight":38756,"crc64":"334487C2D0D30463","md5":"1FD4FF7F3D4C3AA8F00C070B5B84633F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":8,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI0010FF75C7"}}]}