{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HM75","uniProtkbId":"KA106_CENBO","entryAudit":{"firstPublicDate":"2024-01-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-01-24","entryVersion":7,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"Potassium channel toxin alpha-KTx 10.6"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"CboK2"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Blocks human voltage-gated potassium (Kv) channels Kv1.2/KCNA2 (Kd=0.76 nM) and Kv1.3/KCNA3 (Kd=171 nM)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"37624263"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":3760.4,"molWeightError":0.0,"note":"Monoisotopic mass.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Negative results: Does not block human Kv1.1/KCNA1 at 100 nM concentration"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 10 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"Potassium channel toxin alpha-KTx 10.6","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"featureId":"PRO_0000459535"},{"type":"Site","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Basic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Site","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"Aromatic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0632","category":"Molecular function","name":"Potassium channel impairing toxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-1220","category":"Molecular function","name":"Voltage-gated potassium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"37624263","citationType":"journal article","authors":["Shakeel K.","Olamendi-Portugal T.","Naseem M.U.","Becerril B.","Zamudio F.Z.","Delgado-Prudencio G.","Possani L.D.","Panyi G."],"citationCrossReferences":[{"database":"PubMed","id":"37624263"},{"database":"DOI","id":"10.3390/toxins15080506"}],"title":"Of Seven New K+ Channel Inhibitor Peptides of Centruroides bonito, alpha-KTx 2.24 Has a Picomolar Affinity for Kv1.2.","publicationDate":"2023","journal":"Toxins","firstPage":"506","lastPage":"506","volume":"15"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","MASS SPECTROMETRY"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HM75","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HM75","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"database":"GO","id":"GO:0008200","properties":[{"key":"GoTerm","value":"F:ion channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR001947","properties":[{"key":"EntryName","value":"Scorpion_toxinS_K_inh"}]},{"database":"Pfam","id":"PF00451","properties":[{"key":"EntryName","value":"Toxin_2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"TVCVYRTCDKDCKRRGYRSGKCINNACKCYPY","length":32,"molWeight":3768,"crc64":"C20340A309D82F9C","md5":"F7D514AB9B3442DAE664CBC4D6B3C472"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Site":2,"Disulfide bond":3},"uniParcId":"UPI00298BC915"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HM77","uniProtkbId":"KAX2M_CENBO","entryAudit":{"firstPublicDate":"2024-01-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-01-24","entryVersion":7,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"Potassium channel toxin alpha-KTx 2.22"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"CboK3"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Inhibits human voltage-gated potassium (Kv) channels Kv1.1/KCNA1 (Kd=1 uM), Kv1.2/KCNA2 (Kd=0.106 nM) and Kv1.3/KCNA3 (Kd=34.3 nM)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"37624263"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P40755"}],"value":"Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":4323.5,"molWeightError":0.0,"note":"Monoisotopic mass.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"The primary structure of the mature protein is identical to that of Ce3 from Centruroides elegans (AC P0C163)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 02 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"Potassium channel toxin alpha-KTx 2.22","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"featureId":"PRO_0000459537"},{"type":"Site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Basic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"Aromatic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0632","category":"Molecular function","name":"Potassium channel impairing toxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-1220","category":"Molecular function","name":"Voltage-gated potassium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"37624263","citationType":"journal article","authors":["Shakeel K.","Olamendi-Portugal T.","Naseem M.U.","Becerril B.","Zamudio F.Z.","Delgado-Prudencio G.","Possani L.D.","Panyi G."],"citationCrossReferences":[{"database":"PubMed","id":"37624263"},{"database":"DOI","id":"10.3390/toxins15080506"}],"title":"Of Seven New K+ Channel Inhibitor Peptides of Centruroides bonito, alpha-KTx 2.24 Has a Picomolar Affinity for Kv1.2.","publicationDate":"2023","journal":"Toxins","firstPage":"506","lastPage":"506","volume":"15"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","MASS SPECTROMETRY"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HM77","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HM77","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"database":"GO","id":"GO:0008200","properties":[{"key":"GoTerm","value":"F:ion channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR001947","properties":[{"key":"EntryName","value":"Scorpion_toxinS_K_inh"}]},{"database":"Pfam","id":"PF00451","properties":[{"key":"EntryName","value":"Toxin_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00286","properties":[{"key":"EntryName","value":"CHARYBDTOXIN"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"TIINVKCTSPKQCLKPCKDLYGPHAGEKCMNGKCKCYKV","length":39,"molWeight":4331,"crc64":"F6F9D54889860E10","md5":"1A1B4475C9C1D381FC6B9135E9D29577"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Site":2,"Disulfide bond":3},"uniParcId":"UPI00298BC8E7"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HMA3","uniProtkbId":"SCX1_CENBO","entryAudit":{"firstPublicDate":"2024-10-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-10-02","entryVersion":7,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Beta-toxin Cbo1"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Cbo1"}]}},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Beta toxins bind voltage-independently at site-4 of sodium channels and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (PubMed:38535792). Is active on the human voltage-gated sodium channel Nav1.6/SCN8A when tested at 200 nM (PubMed:38535792). In vivo, is toxic to mice when intraperitoneally injected (PubMed:38535792)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"38535792"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":7586.64,"molWeightError":0.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Negative results: does not modify the currents of the human voltage-gated sodium channels Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A, Nav1.4/SCN4A, Nav1.5/SCN5A and Nav1.7/SCN9A"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Beta-toxin Cbo1","featureId":"PRO_0000461089"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"LCN-type CS-alpha/beta","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Modified residue","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Asparagine amide","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P59897"}]},{"type":"Disulfide bond","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]}],"keywords":[{"id":"KW-0027","category":"PTM","name":"Amidation"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-0738","category":"Molecular function","name":"Voltage-gated sodium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"38535792","citationType":"journal article","authors":["Restano-Cassulini R.","Olamendi-Portugal T.","Riano-Umbarila L.","Zamudio F.Z.","Delgado-Prudencio G.","Becerril B.","Possani L.D."],"citationCrossReferences":[{"database":"PubMed","id":"38535792"},{"database":"DOI","id":"10.3390/toxins16030125"}],"title":"Characterization of Sodium Channel Peptides Obtained from the Venom of the Scorpion Centruroides bonito.","publicationDate":"2024","journal":"Toxins","firstPage":"0","lastPage":"0","volume":"16"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","MASS SPECTROMETRY"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HMA3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HMA3","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"database":"GO","id":"GO:0019871","properties":[{"key":"GoTerm","value":"F:sodium channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0090729","properties":[{"key":"GoTerm","value":"F:toxin activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006952","properties":[{"key":"GoTerm","value":"P:defense response"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd23106","properties":[{"key":"EntryName","value":"neurotoxins_LC_scorpion"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.30.10:FF:000002","properties":[{"key":"EntryName","value":"Alpha-like toxin BmK-M1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003614","properties":[{"key":"EntryName","value":"Knottins"}]},{"database":"InterPro","id":"IPR044062","properties":[{"key":"EntryName","value":"LCN-type_CS_alpha_beta_dom"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR018218","properties":[{"key":"EntryName","value":"Scorpion_toxinL"}]},{"database":"PRINTS","id":"PR00285","properties":[{"key":"EntryName","value":"SCORPNTOXIN"}]},{"database":"SMART","id":"SM00505","properties":[{"key":"EntryName","value":"Knot1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51863","properties":[{"key":"EntryName","value":"LCN_CSAB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"KEGYLVNHSTGCKYECYKLGDNDYCLRECKQQYGKGAGGYCYAFGCWCTHLYEQAVVWPLPKKTCN","length":66,"molWeight":7587,"crc64":"DF124D41D0A5B0C2","md5":"8097CD562409BA6F356236D8949044C0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Modified residue":1,"Disulfide bond":4},"uniParcId":"UPI00001D6F10"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HMA4","uniProtkbId":"SCX2_CENBO","entryAudit":{"firstPublicDate":"2024-10-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-10-02","entryVersion":7,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Beta-toxin Cbo2"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Cbo2"}]}},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Beta toxins bind voltage-independently at site-4 of sodium channels and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (PubMed:38535792). A mixture of Cbo2 and Cbo3 is weakly active on the human voltage-gated sodium channels Nav1.4/SCN4A and Nav1.6/SCN8A when tested at 200 nM (PubMed:38535792). In vivo, is toxic to mice when intraperitoneally injected (PubMed:38535792)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"38535792"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":7675.74,"molWeightError":0.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Negative results: a mixture of Cbo2 and Cbo3 does not modify the currents of the human voltage-gated sodium channels Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A, Nav1.5/SCN5A and Nav1.7/SCN9A"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Beta-toxin Cbo2","featureId":"PRO_0000461090"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"LCN-type CS-alpha/beta","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Modified residue","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Asparagine amide","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q7Z1K8"}]},{"type":"Disulfide bond","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]}],"keywords":[{"id":"KW-0027","category":"PTM","name":"Amidation"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-0738","category":"Molecular function","name":"Voltage-gated sodium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"38535792","citationType":"journal article","authors":["Restano-Cassulini R.","Olamendi-Portugal T.","Riano-Umbarila L.","Zamudio F.Z.","Delgado-Prudencio G.","Becerril B.","Possani L.D."],"citationCrossReferences":[{"database":"PubMed","id":"38535792"},{"database":"DOI","id":"10.3390/toxins16030125"}],"title":"Characterization of Sodium Channel Peptides Obtained from the Venom of the Scorpion Centruroides bonito.","publicationDate":"2024","journal":"Toxins","firstPage":"0","lastPage":"0","volume":"16"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","MASS SPECTROMETRY"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HMA4","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HMA4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"database":"GO","id":"GO:0019871","properties":[{"key":"GoTerm","value":"F:sodium channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0090729","properties":[{"key":"GoTerm","value":"F:toxin activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006952","properties":[{"key":"GoTerm","value":"P:defense response"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd23106","properties":[{"key":"EntryName","value":"neurotoxins_LC_scorpion"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.30.10:FF:000002","properties":[{"key":"EntryName","value":"Alpha-like toxin BmK-M1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003614","properties":[{"key":"EntryName","value":"Knottins"}]},{"database":"InterPro","id":"IPR044062","properties":[{"key":"EntryName","value":"LCN-type_CS_alpha_beta_dom"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR018218","properties":[{"key":"EntryName","value":"Scorpion_toxinL"}]},{"database":"PRINTS","id":"PR00285","properties":[{"key":"EntryName","value":"SCORPNTOXIN"}]},{"database":"SMART","id":"SM00505","properties":[{"key":"EntryName","value":"Knot1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51863","properties":[{"key":"EntryName","value":"LCN_CSAB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"KEGYIVNYHDGCKYECYKLGDNDYCLRECRARYGKGAGGYCYAFGCWCTHLYEQAVVWPLPKKTCN","length":66,"molWeight":7676,"crc64":"C86E35A75393C0F2","md5":"2CCB0D70692AF82BB1DA764E197EA49D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Modified residue":1,"Disulfide bond":4},"uniParcId":"UPI0033907849"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HM72","uniProtkbId":"KAX2N_CENBO","entryAudit":{"firstPublicDate":"2024-01-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-01-24","entryVersion":8,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"Potassium channel toxin alpha-KTx 2.23"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"CboK4"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Blocks human voltage-gated potassium (Kv) channels Kv1.1/KCNA1 (Kd=1.5 uM), Kv1.2/KCNA2 (Kd=0.125 nM) and Kv1.3/KCNA3 (Kd=21.7 nM)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"37624263"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P08815"}],"value":"Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":4357.9,"molWeightError":0.0,"note":"Monoisotopic mass.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 02 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"Potassium channel toxin alpha-KTx 2.23","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"featureId":"PRO_0000459532"},{"type":"Site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Basic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"Aromatic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P08815"}]},{"type":"Disulfide bond","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P08815"}]},{"type":"Disulfide bond","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P08815"}]}],"keywords":[{"id":"KW-1221","category":"Molecular function","name":"Calcium-activated potassium channel impairing toxin"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0632","category":"Molecular function","name":"Potassium channel impairing toxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-1220","category":"Molecular function","name":"Voltage-gated potassium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"37624263","citationType":"journal article","authors":["Shakeel K.","Olamendi-Portugal T.","Naseem M.U.","Becerril B.","Zamudio F.Z.","Delgado-Prudencio G.","Possani L.D.","Panyi G."],"citationCrossReferences":[{"database":"PubMed","id":"37624263"},{"database":"DOI","id":"10.3390/toxins15080506"}],"title":"Of Seven New K+ Channel Inhibitor Peptides of Centruroides bonito, alpha-KTx 2.24 Has a Picomolar Affinity for Kv1.2.","publicationDate":"2023","journal":"Toxins","firstPage":"506","lastPage":"506","volume":"15"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","MASS SPECTROMETRY"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HM72","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HM72","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"database":"GO","id":"GO:0008200","properties":[{"key":"GoTerm","value":"F:ion channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"FunFam","id":"3.30.30.10:FF:000009","properties":[{"key":"EntryName","value":"Potassium channel toxin alpha-KTx 4.3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR001947","properties":[{"key":"EntryName","value":"Scorpion_toxinS_K_inh"}]},{"database":"Pfam","id":"PF00451","properties":[{"key":"EntryName","value":"Toxin_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00286","properties":[{"key":"EntryName","value":"CHARYBDTOXIN"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"TFINVKCTSPKQCLKPCKDLYGPHAGEKCMNGKCKCYKP","length":39,"molWeight":4363,"crc64":"3059D548898F0780","md5":"45D671CCF961CA7FF71F6E8C10D79A9E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Site":2,"Disulfide bond":3},"uniParcId":"UPI00298BC8F4"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HM73","uniProtkbId":"KA105_CENBO","entryAudit":{"firstPublicDate":"2024-01-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-01-24","entryVersion":7,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"Potassium channel toxin alpha-KTx 10.5"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"CboK1"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"37624263"}],"value":"Toxin with unknown function"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"37624263"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":3765.1,"molWeightError":0.0,"note":"Monoisotopic mass.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Negative results: inhibits less than 5% of human voltage-gated potassium (Kv) channel Kv1.3/KCNA3 currents at 100 nM concentration and does not block human Kv1.1/KCNA1 and Kv1.2/KCNA2 currents"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 10 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"Potassium channel toxin alpha-KTx 10.5","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"featureId":"PRO_0000459533"},{"type":"Site","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"Basic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Site","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"Aromatic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0632","category":"Molecular function","name":"Potassium channel impairing toxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-1220","category":"Molecular function","name":"Voltage-gated potassium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"37624263","citationType":"journal article","authors":["Shakeel K.","Olamendi-Portugal T.","Naseem M.U.","Becerril B.","Zamudio F.Z.","Delgado-Prudencio G.","Possani L.D.","Panyi G."],"citationCrossReferences":[{"database":"PubMed","id":"37624263"},{"database":"DOI","id":"10.3390/toxins15080506"}],"title":"Of Seven New K+ Channel Inhibitor Peptides of Centruroides bonito, alpha-KTx 2.24 Has a Picomolar Affinity for Kv1.2.","publicationDate":"2023","journal":"Toxins","firstPage":"506","lastPage":"506","volume":"15"},"referencePositions":["PROTEIN SEQUENCE","SUBCELLULAR LOCATION","MASS SPECTROMETRY"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HM73","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HM73","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"database":"GO","id":"GO:0008200","properties":[{"key":"GoTerm","value":"F:ion channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR001947","properties":[{"key":"EntryName","value":"Scorpion_toxinS_K_inh"}]},{"database":"Pfam","id":"PF00451","properties":[{"key":"EntryName","value":"Toxin_2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"NVACVHRTCDSNCKRNGYKSGKCINRKCNCYPH","length":33,"molWeight":3773,"crc64":"66E607D31FA4562E","md5":"5EBFE90AA7217CCE2DC06ED60B162C34"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Site":2,"Disulfide bond":3},"uniParcId":"UPI00298BC8FA"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HMA5","uniProtkbId":"SCX3_CENBO","entryAudit":{"firstPublicDate":"2024-10-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-10-02","entryVersion":7,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Beta-toxin Cbo3"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Cbo3"}]}},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Beta toxins bind voltage-independently at site-4 of sodium channels and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (PubMed:38535792). A mixture of Cbo2 and Cbo3 is weakly active on the human voltage-gated sodium channels Nav1.4/SCN4A and Nav1.6/SCN8A when tested at 200 nM (PubMed:38535792). In vivo, is toxic to mice when intraperitoneally injected (PubMed:38535792)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"38535792"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":7647.73,"molWeightError":0.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Negative results: a mixture of Cbo2 and Cbo3 does not modify the currents of the human voltage-gated sodium channels Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A, Nav1.5/SCN5A and Nav1.7/SCN9A"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Beta-toxin Cbo3","featureId":"PRO_0000461091"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"LCN-type CS-alpha/beta","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Modified residue","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Asparagine amide","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q7Z1K8"}]},{"type":"Disulfide bond","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]}],"keywords":[{"id":"KW-0027","category":"PTM","name":"Amidation"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-0738","category":"Molecular function","name":"Voltage-gated sodium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"38535792","citationType":"journal article","authors":["Restano-Cassulini R.","Olamendi-Portugal T.","Riano-Umbarila L.","Zamudio F.Z.","Delgado-Prudencio G.","Becerril B.","Possani L.D."],"citationCrossReferences":[{"database":"PubMed","id":"38535792"},{"database":"DOI","id":"10.3390/toxins16030125"}],"title":"Characterization of Sodium Channel Peptides Obtained from the Venom of the Scorpion Centruroides bonito.","publicationDate":"2024","journal":"Toxins","firstPage":"0","lastPage":"0","volume":"16"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","MASS SPECTROMETRY"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HMA5","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HMA5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"database":"GO","id":"GO:0019871","properties":[{"key":"GoTerm","value":"F:sodium channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0090729","properties":[{"key":"GoTerm","value":"F:toxin activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006952","properties":[{"key":"GoTerm","value":"P:defense response"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd23106","properties":[{"key":"EntryName","value":"neurotoxins_LC_scorpion"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.30.10:FF:000002","properties":[{"key":"EntryName","value":"Alpha-like toxin BmK-M1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003614","properties":[{"key":"EntryName","value":"Knottins"}]},{"database":"InterPro","id":"IPR044062","properties":[{"key":"EntryName","value":"LCN-type_CS_alpha_beta_dom"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR018218","properties":[{"key":"EntryName","value":"Scorpion_toxinL"}]},{"database":"PRINTS","id":"PR00285","properties":[{"key":"EntryName","value":"SCORPNTOXIN"}]},{"database":"SMART","id":"SM00505","properties":[{"key":"EntryName","value":"Knot1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51863","properties":[{"key":"EntryName","value":"LCN_CSAB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"KEGYIVNYHDGCKYECYKLGDNDYCLRECKARYGKGAGGYCYAFGCWCTHLYEQAVVWPLPKKTCN","length":66,"molWeight":7648,"crc64":"C86E35A745D1A4C2","md5":"1ED9B14769AA68B22C7FB63F69326DD8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Modified residue":1,"Disulfide bond":4},"uniParcId":"UPI0033907846"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HMA6","uniProtkbId":"SCX4_CENBO","entryAudit":{"firstPublicDate":"2024-10-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-10-02","entryVersion":7,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Beta-toxin Cbo4"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Cbo4"}]}},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Beta toxins bind voltage-independently at site-4 of sodium channels and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (PubMed:38535792). Is active on the human voltage-gated sodium channels Nav1.4/SCN4A, Nav1.5/SCN5A and Nav1.6/SCN8A when tested at 200 nM (PubMed:38535792). In vivo, is toxic to mice when intraperitoneally injected (PubMed:38535792)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"38535792"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":7855.99,"molWeightError":0.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Negative results: does not modify the currents of the human voltage-gated sodium channels Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A and Nav1.7/SCN9A"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Beta-toxin Cbo4","featureId":"PRO_0000461092"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"LCN-type CS-alpha/beta","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Modified residue","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Lysine amide","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"C0HK69"}]},{"type":"Disulfide bond","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]}],"keywords":[{"id":"KW-0027","category":"PTM","name":"Amidation"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-0738","category":"Molecular function","name":"Voltage-gated sodium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"38535792","citationType":"journal article","authors":["Restano-Cassulini R.","Olamendi-Portugal T.","Riano-Umbarila L.","Zamudio F.Z.","Delgado-Prudencio G.","Becerril B.","Possani L.D."],"citationCrossReferences":[{"database":"PubMed","id":"38535792"},{"database":"DOI","id":"10.3390/toxins16030125"}],"title":"Characterization of Sodium Channel Peptides Obtained from the Venom of the Scorpion Centruroides bonito.","publicationDate":"2024","journal":"Toxins","firstPage":"0","lastPage":"0","volume":"16"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","MASS SPECTROMETRY"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HMA6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HMA6","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"database":"GO","id":"GO:0019871","properties":[{"key":"GoTerm","value":"F:sodium channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0090729","properties":[{"key":"GoTerm","value":"F:toxin activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006952","properties":[{"key":"GoTerm","value":"P:defense response"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd23106","properties":[{"key":"EntryName","value":"neurotoxins_LC_scorpion"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.30.10:FF:000002","properties":[{"key":"EntryName","value":"Alpha-like toxin BmK-M1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003614","properties":[{"key":"EntryName","value":"Knottins"}]},{"database":"InterPro","id":"IPR044062","properties":[{"key":"EntryName","value":"LCN-type_CS_alpha_beta_dom"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR018218","properties":[{"key":"EntryName","value":"Scorpion_toxinL"}]},{"database":"InterPro","id":"IPR002061","properties":[{"key":"EntryName","value":"Scorpion_toxinL/defensin"}]},{"database":"Pfam","id":"PF00537","properties":[{"key":"EntryName","value":"Toxin_3"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00285","properties":[{"key":"EntryName","value":"SCORPNTOXIN"}]},{"database":"SMART","id":"SM00505","properties":[{"key":"EntryName","value":"Knot1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51863","properties":[{"key":"EntryName","value":"LCN_CSAB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"KEGYIVDYHTGCKYTCAKLGDNDYCVRECRLRYYQSAHGYCYAFACWCTHLYEQAVVWPLPNKRCK","length":66,"molWeight":7856,"crc64":"4EB85995115FA0F2","md5":"F41F6B9685D13CE8955BE6CBF6AF163E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Modified residue":1,"Disulfide bond":4},"uniParcId":"UPI0033907861"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HMA7","uniProtkbId":"SCX5_CENBO","entryAudit":{"firstPublicDate":"2024-10-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-10-02","entryVersion":7,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Toxin Cbo5"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Cbo5"}]}},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"},{"evidenceCode":"ECO:0000305"}],"value":"A probable toxin that has no activity on the tested sodium channels (when tested at 200 nM) and is not toxic to mice, crickets or sweet water shrimps (PubMed:38535792). It resembles Beta toxins that bind voltage-independently at site-4 of sodium channels and shift the voltage of activation toward more negative potentials, thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (Probable)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"38535792"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":7493.47,"molWeightError":0.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}],"value":"Negative results: does not modify the currents of the human voltage-gated sodium channels Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A, Nav 1.4/SCN4A, Nav1.5/SCN5A, Nav1.6/SCN8A and Nav1.7/SCN9A"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Toxin Cbo5","featureId":"PRO_0000461093"},{"type":"Domain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"LCN-type CS-alpha/beta","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]},{"type":"Disulfide bond","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01210"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"38535792","citationType":"journal article","authors":["Restano-Cassulini R.","Olamendi-Portugal T.","Riano-Umbarila L.","Zamudio F.Z.","Delgado-Prudencio G.","Becerril B.","Possani L.D."],"citationCrossReferences":[{"database":"PubMed","id":"38535792"},{"database":"DOI","id":"10.3390/toxins16030125"}],"title":"Characterization of Sodium Channel Peptides Obtained from the Venom of the Scorpion Centruroides bonito.","publicationDate":"2024","journal":"Toxins","firstPage":"0","lastPage":"0","volume":"16"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","MASS SPECTROMETRY"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38535792"}],"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HMA7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HMA7","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38535792"}]},{"database":"GO","id":"GO:0019871","properties":[{"key":"GoTerm","value":"F:sodium channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0090729","properties":[{"key":"GoTerm","value":"F:toxin activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006952","properties":[{"key":"GoTerm","value":"P:defense response"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd23106","properties":[{"key":"EntryName","value":"neurotoxins_LC_scorpion"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.30.10:FF:000002","properties":[{"key":"EntryName","value":"Alpha-like toxin BmK-M1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003614","properties":[{"key":"EntryName","value":"Knottins"}]},{"database":"InterPro","id":"IPR044062","properties":[{"key":"EntryName","value":"LCN-type_CS_alpha_beta_dom"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR018218","properties":[{"key":"EntryName","value":"Scorpion_toxinL"}]},{"database":"InterPro","id":"IPR002061","properties":[{"key":"EntryName","value":"Scorpion_toxinL/defensin"}]},{"database":"Pfam","id":"PF00537","properties":[{"key":"EntryName","value":"Toxin_3"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00285","properties":[{"key":"EntryName","value":"SCORPNTOXIN"}]},{"database":"SMART","id":"SM00505","properties":[{"key":"EntryName","value":"Knot1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51863","properties":[{"key":"EntryName","value":"LCN_CSAB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"KKDGYLVDKTGCKYTCWKLGENKYCNRECTWKHRGGNYGYCYGFGCYCEGLADSTQTWPLPNKSC","length":65,"molWeight":7493,"crc64":"64B34EA61358F7DC","md5":"1263EB8AB6FF2365F49558AAD5D8AC2E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Disulfide bond":4},"uniParcId":"UPI003390784E"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HM74","uniProtkbId":"KAX2A_CENBO","entryAudit":{"firstPublicDate":"2024-01-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-01-24","entryVersion":8,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"Potassium channel toxin alpha-KTx 2.10"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"CboK5"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Blocks human voltage-gated potassium (Kv) channel Kv1.2/KCNA2 (Kd=0.376 nM) and Kv1.3/KCNA3 (Kd=126 nM)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"37624263"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":4249.8,"molWeightError":0.0,"note":"Monoisotopic mass.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Negative results: does not inhibit human Kv1.1/KCNA1 at 100 nM concentration"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 02 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"Potassium channel toxin alpha-KTx 2.10","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"featureId":"PRO_0000459534"},{"type":"Site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Basic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"Aromatic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P40755"}]},{"type":"Disulfide bond","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P40755"}]},{"type":"Disulfide bond","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P40755"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0632","category":"Molecular function","name":"Potassium channel impairing toxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-1220","category":"Molecular function","name":"Voltage-gated potassium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"37624263","citationType":"journal article","authors":["Shakeel K.","Olamendi-Portugal T.","Naseem M.U.","Becerril B.","Zamudio F.Z.","Delgado-Prudencio G.","Possani L.D.","Panyi G."],"citationCrossReferences":[{"database":"PubMed","id":"37624263"},{"database":"DOI","id":"10.3390/toxins15080506"}],"title":"Of Seven New K+ Channel Inhibitor Peptides of Centruroides bonito, alpha-KTx 2.24 Has a Picomolar Affinity for Kv1.2.","publicationDate":"2023","journal":"Toxins","firstPage":"506","lastPage":"506","volume":"15"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","MASS SPECTROMETRY"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HM74","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HM74","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"database":"GO","id":"GO:0008200","properties":[{"key":"GoTerm","value":"F:ion channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"FunFam","id":"3.30.30.10:FF:000009","properties":[{"key":"EntryName","value":"Potassium channel toxin alpha-KTx 4.3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR001947","properties":[{"key":"EntryName","value":"Scorpion_toxinS_K_inh"}]},{"database":"Pfam","id":"PF00451","properties":[{"key":"EntryName","value":"Toxin_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00286","properties":[{"key":"EntryName","value":"CHARYBDTOXIN"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"IFINVKCSLPQQCLRPCKDRFGQHAGGKCINGKCKCYP","length":38,"molWeight":4255,"crc64":"CE6E33F704B398F6","md5":"94AF5A0EED0FA75E9686205B6188E88F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Site":2,"Disulfide bond":3},"uniParcId":"UPI000069649F"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HM76","uniProtkbId":"KAX2L_CENBO","entryAudit":{"firstPublicDate":"2024-01-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2024-01-24","entryVersion":8,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"Potassium channel toxin alpha-KTx 2.21"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"CboK6"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Inhibits human voltage-gated potassium (Kv) channels Kv1.2/KCNA2 (Kd=0.585 nM) and Kv1.3/KCNA3 (Kd=160 nM)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"37624263"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P40755"}],"value":"Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":4191.3,"molWeightError":0.0,"note":"Monoisotopic mass.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Negative results: does not block human Kv1.1/KCNA1 at 100 nM concentration"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 02 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"Potassium channel toxin alpha-KTx 2.21","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"featureId":"PRO_0000459536"},{"type":"Site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Basic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"Aromatic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0632","category":"Molecular function","name":"Potassium channel impairing toxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-1220","category":"Molecular function","name":"Voltage-gated potassium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"37624263","citationType":"journal article","authors":["Shakeel K.","Olamendi-Portugal T.","Naseem M.U.","Becerril B.","Zamudio F.Z.","Delgado-Prudencio G.","Possani L.D.","Panyi G."],"citationCrossReferences":[{"database":"PubMed","id":"37624263"},{"database":"DOI","id":"10.3390/toxins15080506"}],"title":"Of Seven New K+ Channel Inhibitor Peptides of Centruroides bonito, alpha-KTx 2.24 Has a Picomolar Affinity for Kv1.2.","publicationDate":"2023","journal":"Toxins","firstPage":"506","lastPage":"506","volume":"15"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","MASS SPECTROMETRY"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HM76","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HM76","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"database":"GO","id":"GO:0008200","properties":[{"key":"GoTerm","value":"F:ion channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"FunFam","id":"3.30.30.10:FF:000009","properties":[{"key":"EntryName","value":"Potassium channel toxin alpha-KTx 4.3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR001947","properties":[{"key":"EntryName","value":"Scorpion_toxinS_K_inh"}]},{"database":"Pfam","id":"PF00451","properties":[{"key":"EntryName","value":"Toxin_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00286","properties":[{"key":"EntryName","value":"CHARYBDTOXIN"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"TTINVKCSLPQQCLRPCKDRFGQHAGGKCINGKCKCYP","length":38,"molWeight":4197,"crc64":"576E33F71D44E898","md5":"BD53229AF2CC3209E119205E49521922"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Site":2,"Disulfide bond":3},"uniParcId":"UPI00298BC8F1"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C0HM78","uniProtkbId":"KAX2O_CENBO","entryAudit":{"firstPublicDate":"2024-01-24","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2026-01-28","entryVersion":9,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Centruroides bonito","commonName":"Bark scorpion","taxonId":3035065,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Chelicerata","Arachnida","Scorpiones","Buthida","Buthoidea","Buthidae","Centruroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"Potassium channel toxin alpha-KTx 2.24"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37624263"}],"value":"CboK7"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Blocks human voltage-gated potassium (Kv) channels Kv1.1/KCNA (Kd=141 nM), Kv1.2/KCNA2 (Kd=0.024 nM) and Kv1.3/KCNA3 (Kd=20.4 nM)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"37624263"}],"value":"Expressed by the venom gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P08815"}],"value":"Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta)"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":4298.5,"molWeightError":0.0,"note":"Monoisotopic mass.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 02 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"Potassium channel toxin alpha-KTx 2.24","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}],"featureId":"PRO_0000459538"},{"type":"Site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Basic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"Aromatic residue of the functional dyad","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]},{"type":"Disulfide bond","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O46028"}]}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0872","category":"Molecular function","name":"Ion channel impairing toxin"},{"id":"KW-0528","category":"Molecular function","name":"Neurotoxin"},{"id":"KW-0582","category":"Technical term","name":"Pharmaceutical"},{"id":"KW-0632","category":"Molecular function","name":"Potassium channel impairing toxin"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-1220","category":"Molecular function","name":"Voltage-gated potassium channel impairing toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"37624263","citationType":"journal article","authors":["Shakeel K.","Olamendi-Portugal T.","Naseem M.U.","Becerril B.","Zamudio F.Z.","Delgado-Prudencio G.","Possani L.D.","Panyi G."],"citationCrossReferences":[{"database":"PubMed","id":"37624263"},{"database":"DOI","id":"10.3390/toxins15080506"}],"title":"Of Seven New K+ Channel Inhibitor Peptides of Centruroides bonito, alpha-KTx 2.24 Has a Picomolar Affinity for Kv1.2.","publicationDate":"2023","journal":"Toxins","firstPage":"506","lastPage":"506","volume":"15"},"referencePositions":["PROTEIN SEQUENCE","FUNCTION","SUBCELLULAR LOCATION","MASS SPECTROMETRY"],"referenceComments":[{"value":"Venom","type":"TISSUE"}],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":2,"citation":{"id":"CI-9OTFM1IEQIEOP","citationType":"unpublished observations","authors":["Shakeel K."],"publicationDate":"APR-2025"},"referencePositions":["SEQUENCE REVISION TO 15; 19-20; 23 AND 27"]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"C0HM78","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C0HM78","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37624263"}]},{"database":"GO","id":"GO:0008200","properties":[{"key":"GoTerm","value":"F:ion channel inhibitor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"FunFam","id":"3.30.30.10:FF:000009","properties":[{"key":"EntryName","value":"Potassium channel toxin alpha-KTx 4.3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.30.10","properties":[{"key":"EntryName","value":"Knottin, scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036574","properties":[{"key":"EntryName","value":"Scorpion_toxin-like_sf"}]},{"database":"InterPro","id":"IPR001947","properties":[{"key":"EntryName","value":"Scorpion_toxinS_K_inh"}]},{"database":"Pfam","id":"PF00451","properties":[{"key":"EntryName","value":"Toxin_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00286","properties":[{"key":"EntryName","value":"CHARYBDTOXIN"}]},{"database":"SUPFAM","id":"SSF57095","properties":[{"key":"EntryName","value":"Scorpion toxin-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"TFINVKCTSPKQCLPPCKEIYGIHAGAKCMNGKCKCYKV","length":39,"molWeight":4306,"crc64":"3DFF8E4E3BF07780","md5":"3A1744522FD33C38EC390C46AFAB419A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"MASS SPECTROMETRY":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Site":2,"Disulfide bond":3},"uniParcId":"UPI004074769D"}}]}