{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q59270","uniProtkbId":"NIFH_RUMCE","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-01-23","entryVersion":108,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Ruminiclostridium cellobioparum","commonName":"Clostridium cellobioparum","taxonId":29355,"lineage":["Bacteria","Bacillati","Bacillota","Clostridia","Eubacteriales","Oscillospiraceae","Ruminiclostridium"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Nitrogenase iron protein"},"ecNumbers":[{"value":"1.18.6.1"}]},"alternativeNames":[{"fullName":{"value":"Nitrogenase Fe protein"}},{"fullName":{"value":"Nitrogenase component II"}},{"fullName":{"value":"Nitrogenase reductase"}}]},"genes":[{"geneName":{"value":"nifH"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N2 + 8 reduced [2Fe-2S]-[ferredoxin] + 16 ATP + 16 H2O = H2 + 8 oxidized [2Fe-2S]-[ferredoxin] + 2 NH4(+) + 16 ADP + 16 phosphate + 6 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21448"},{"database":"Rhea","id":"RHEA-COMP:10000"},{"database":"Rhea","id":"RHEA-COMP:10001"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17997"},{"database":"ChEBI","id":"CHEBI:18276"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33737"},{"database":"ChEBI","id":"CHEBI:33738"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"1.18.6.1"}},{"commentType":"COFACTOR","cofactors":[{"name":"[4Fe-4S] cluster","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49883"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 1 [4Fe-4S] cluster per dimer."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"The reversible ADP-ribosylation of Arg-97 inactivates the nitrogenase reductase and regulates nitrogenase activity"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the NifH/BchL/ChlL family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"AAB63257.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"Nitrogenase iron protein","featureId":"PRO_0000139497"},{"type":"Binding site","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":129,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","note":"ligand shared between dimeric partners"}},{"type":"Modified residue","location":{"start":{"value":97,"modifier":"EXACT"},"end":{"value":97,"modifier":"EXACT"}},"description":"ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase","evidences":[{"evidenceCode":"ECO:0000250"}]}],"keywords":[{"id":"KW-0004","category":"Ligand","name":"4Fe-4S"},{"id":"KW-0013","category":"PTM","name":"ADP-ribosylation"},{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0535","category":"Biological process","name":"Nitrogen fixation"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-CR94Q2B9TOJQL","citationType":"submission","authors":["Chen T.","Leschine S.B."],"title":"Cloning of two potential nif genes from cellulolytic clostridia.","publicationDate":"JUN-1996","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 15832 / DSM 1351 / BCRC 14495 / JCM 1422 / NCIMB 10669 / VPI 3359 / Cc","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U59414","properties":[{"key":"ProteinId","value":"AAB63257.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q59270","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q59270","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016163","properties":[{"key":"GoTerm","value":"F:nitrogenase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd02040","properties":[{"key":"EntryName","value":"NifH"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00533","properties":[{"key":"EntryName","value":"NifH"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR030655","properties":[{"key":"EntryName","value":"NifH/chlL_CS"}]},{"database":"InterPro","id":"IPR000392","properties":[{"key":"EntryName","value":"NifH/frxC"}]},{"database":"InterPro","id":"IPR005977","properties":[{"key":"EntryName","value":"Nitrogenase_Fe_NifH"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"NCBIfam","id":"TIGR01287","properties":[{"key":"EntryName","value":"nifH"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42864","properties":[{"key":"EntryName","value":"LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE IRON-SULFUR ATP-BINDING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42864:SF2","properties":[{"key":"EntryName","value":"LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE IRON-SULFUR ATP-BINDING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00142","properties":[{"key":"EntryName","value":"Fer4_NifH"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000363","properties":[{"key":"EntryName","value":"Nitrogenase_iron"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00091","properties":[{"key":"EntryName","value":"NITROGNASEII"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00746","properties":[{"key":"EntryName","value":"NIFH_FRXC_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00692","properties":[{"key":"EntryName","value":"NIFH_FRXC_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51026","properties":[{"key":"EntryName","value":"NIFH_FRXC_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRQVAIYGKGGIGKSTTTQNLTAGLGEMGKKIMIVGCDPKADSTRLVLGGLAQKTVLDTLREEGEDIELDTVLKVGYAGIKGVESGGPEPGVGCAGRGIITSIGLLERLGAYEADLDYVFYDVLGDVVCGGFAMPIREGKAQEIYIVCSAEMMGLYAANNIAKGISKYANTGGVRLGGLICNSRKVDGEADLVSRVAKEIGTQMIHFVPATMRCRRRKSIKRQLSTFRPMTQADEYRTLARKIDGNDMFVVPRPMSIDRLEAILMEHGILD","length":271,"molWeight":29199,"crc64":"1AFEFB43C674479D","md5":"CEAD0894BCC9F696D84AE212C9793E09"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBUNIT":1,"PTM":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Binding site":3,"Modified residue":1},"uniParcId":"UPI00001300C3"}}]}