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Activity is strongly affected by Hg(2+) and p-chloromercuriphenylsulfonate, but not by N-ethylmaleimide and 5,5'-dithiobis-(2-nitrobenzoate) (PubMed:5646150)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":0.02,"unit":"mM","substrate":"(S)-6-hydroxynicotine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"5849820"}]},{"constant":0.042,"unit":"mM","substrate":"(S)-6-hydroxynicotine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26744768"}]},{"constant":0.064,"unit":"mM","substrate":"(S)-6-hydroxynornicotine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26744768"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26744768"}],"value":"kcat is 30 sec(-1) with (S)-6-hydroxynicotine as substrate. kcat is 16 sec(-1) with (S)-6-hydroxynornicotine as substrate."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"5646150"}],"value":"Fairly stable between pH 6 and 9."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"5849820"}],"value":"Alkaloid degradation; nicotine degradation; 6-hydroxypseudooxynicotine from nicotine (S-isomer route): step 2/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20006620"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"5646150"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"4019415"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"4019415"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"5849820"}],"value":"Induced in the presence of L-nicotine and D-nicotine (PubMed:5849820). 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