{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TRE3","uniProtkbId":"A0A8A4TRE3_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":22,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52546.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"4: Predicted","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52546.1"}],"value":"SDR family NAD(P)-dependent oxidoreductase"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52546.1"}],"value":"J3U87_08740"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023442"}],"value":"Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00054155"}],"value":"Involved in production of the polyketide antibiotic thailandamide"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-butenoyl-[ACP] + NADPH + H(+) = butanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41812"},{"database":"Rhea","id":"RHEA-COMP:9627"},{"database":"Rhea","id":"RHEA-COMP:9628"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78453"},{"database":"ChEBI","id":"CHEBI:78454"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047500"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41813"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047500"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-decenoyl-[ACP] + NADPH + H(+) = decanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41864"},{"database":"Rhea","id":"RHEA-COMP:9639"},{"database":"Rhea","id":"RHEA-COMP:9640"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78467"},{"database":"ChEBI","id":"CHEBI:78468"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049521"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41865"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049521"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-dodecenoyl-[ACP] + NADPH + H(+) = dodecanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41880"},{"database":"Rhea","id":"RHEA-COMP:9643"},{"database":"Rhea","id":"RHEA-COMP:9644"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:65264"},{"database":"ChEBI","id":"CHEBI:78472"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048281"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41881"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048281"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-hexadecenoyl-[ACP] + NADPH + H(+) = hexadecanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41912"},{"database":"Rhea","id":"RHEA-COMP:9651"},{"database":"Rhea","id":"RHEA-COMP:9652"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78481"},{"database":"ChEBI","id":"CHEBI:78483"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047810"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41913"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047810"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-hexenoyl-[ACP] + NADPH + H(+) = hexanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41832"},{"database":"Rhea","id":"RHEA-COMP:9631"},{"database":"Rhea","id":"RHEA-COMP:9632"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78458"},{"database":"ChEBI","id":"CHEBI:78459"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047897"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41833"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047897"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-octadecenoyl-[ACP] + NADPH + H(+) = octadecanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41928"},{"database":"Rhea","id":"RHEA-COMP:9655"},{"database":"Rhea","id":"RHEA-COMP:9656"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78489"},{"database":"ChEBI","id":"CHEBI:78495"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049019"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41929"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049019"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-octenoyl-[ACP] + NADPH + H(+) = octanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41848"},{"database":"Rhea","id":"RHEA-COMP:9635"},{"database":"Rhea","id":"RHEA-COMP:9636"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78462"},{"database":"ChEBI","id":"CHEBI:78463"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048420"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41849"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048420"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-tetradecenoyl-[ACP] + NADPH + H(+) = tetradecanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41896"},{"database":"Rhea","id":"RHEA-COMP:9647"},{"database":"Rhea","id":"RHEA-COMP:9648"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78475"},{"database":"ChEBI","id":"CHEBI:78477"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049171"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41897"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049171"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41808"},{"database":"Rhea","id":"RHEA-COMP:9626"},{"database":"Rhea","id":"RHEA-COMP:9627"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78451"},{"database":"ChEBI","id":"CHEBI:78453"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023402"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41809"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023402"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41860"},{"database":"Rhea","id":"RHEA-COMP:9638"},{"database":"Rhea","id":"RHEA-COMP:9639"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78466"},{"database":"ChEBI","id":"CHEBI:78467"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023388"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41861"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023388"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41876"},{"database":"Rhea","id":"RHEA-COMP:9642"},{"database":"Rhea","id":"RHEA-COMP:9643"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78470"},{"database":"ChEBI","id":"CHEBI:78472"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023351"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41877"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023351"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41908"},{"database":"Rhea","id":"RHEA-COMP:9650"},{"database":"Rhea","id":"RHEA-COMP:9651"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78480"},{"database":"ChEBI","id":"CHEBI:78481"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023401"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41909"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023401"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41828"},{"database":"Rhea","id":"RHEA-COMP:9630"},{"database":"Rhea","id":"RHEA-COMP:9631"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78457"},{"database":"ChEBI","id":"CHEBI:78458"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023373"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41829"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023373"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41924"},{"database":"Rhea","id":"RHEA-COMP:9654"},{"database":"Rhea","id":"RHEA-COMP:9655"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78488"},{"database":"ChEBI","id":"CHEBI:78489"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023399"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41925"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023399"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41844"},{"database":"Rhea","id":"RHEA-COMP:9634"},{"database":"Rhea","id":"RHEA-COMP:9635"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78461"},{"database":"ChEBI","id":"CHEBI:78462"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023332"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41845"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023332"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41892"},{"database":"Rhea","id":"RHEA-COMP:9646"},{"database":"Rhea","id":"RHEA-COMP:9647"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78474"},{"database":"ChEBI","id":"CHEBI:78475"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023398"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41893"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023398"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-oxobutanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxybutanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41804"},{"database":"Rhea","id":"RHEA-COMP:9625"},{"database":"Rhea","id":"RHEA-COMP:9626"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78450"},{"database":"ChEBI","id":"CHEBI:78451"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047953"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41805"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047953"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-oxodecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydecanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41856"},{"database":"Rhea","id":"RHEA-COMP:9637"},{"database":"Rhea","id":"RHEA-COMP:9638"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78464"},{"database":"ChEBI","id":"CHEBI:78466"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047440"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41857"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047440"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-oxododecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydodecanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41872"},{"database":"Rhea","id":"RHEA-COMP:9641"},{"database":"Rhea","id":"RHEA-COMP:9642"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78469"},{"database":"ChEBI","id":"CHEBI:78470"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049263"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41873"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049263"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-oxohexadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexadecanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41904"},{"database":"Rhea","id":"RHEA-COMP:9649"},{"database":"Rhea","id":"RHEA-COMP:9650"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78478"},{"database":"ChEBI","id":"CHEBI:78480"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049414"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41905"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049414"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-oxohexanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41824"},{"database":"Rhea","id":"RHEA-COMP:9629"},{"database":"Rhea","id":"RHEA-COMP:9630"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78456"},{"database":"ChEBI","id":"CHEBI:78457"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048571"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41825"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048571"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-oxooctadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctadecanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41920"},{"database":"Rhea","id":"RHEA-COMP:9653"},{"database":"Rhea","id":"RHEA-COMP:9654"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78487"},{"database":"ChEBI","id":"CHEBI:78488"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047300"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41921"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047300"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-oxooctanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41840"},{"database":"Rhea","id":"RHEA-COMP:9633"},{"database":"Rhea","id":"RHEA-COMP:9634"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78460"},{"database":"ChEBI","id":"CHEBI:78461"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049422"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41841"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049422"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-oxotetradecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxytetradecanoyl-[ACP] + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41888"},{"database":"Rhea","id":"RHEA-COMP:9645"},{"database":"Rhea","id":"RHEA-COMP:9646"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78473"},{"database":"ChEBI","id":"CHEBI:78474"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048935"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41889"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048935"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17397"},{"database":"Rhea","id":"RHEA-COMP:9916"},{"database":"Rhea","id":"RHEA-COMP:9945"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78776"},{"database":"ChEBI","id":"CHEBI:78827"}],"ecNumber":"1.1.1.100","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047400"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:17399"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047400"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13097"},{"database":"Rhea","id":"RHEA-COMP:9925"},{"database":"Rhea","id":"RHEA-COMP:9945"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78784"},{"database":"ChEBI","id":"CHEBI:78827"}],"ecNumber":"4.2.1.59","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023394"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:13098"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023394"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22564"},{"database":"Rhea","id":"RHEA-COMP:9925"},{"database":"Rhea","id":"RHEA-COMP:9926"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78784"},{"database":"ChEBI","id":"CHEBI:78785"}],"ecNumber":"1.3.1.39","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048650"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:22566"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048650"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22836"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"Rhea","id":"RHEA-COMP:9916"},{"database":"Rhea","id":"RHEA-COMP:14125"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78776"},{"database":"ChEBI","id":"CHEBI:138651"}],"ecNumber":"2.3.1.41","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048506"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:22837"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048506"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"acetyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41800"},{"database":"Rhea","id":"RHEA-COMP:9621"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9625"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78446"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78450"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047961"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41801"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047961"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41820"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9628"},{"database":"Rhea","id":"RHEA-COMP:9629"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78454"},{"database":"ChEBI","id":"CHEBI:78456"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049449"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41821"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049449"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41868"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9640"},{"database":"Rhea","id":"RHEA-COMP:9641"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78468"},{"database":"ChEBI","id":"CHEBI:78469"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049109"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41869"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049109"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41884"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9644"},{"database":"Rhea","id":"RHEA-COMP:9645"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:65264"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78473"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047578"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41885"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047578"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41932"},{"database":"Rhea","id":"RHEA-COMP:9652"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:7896"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78483"}],"ecNumber":"3.1.2.14","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048704"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41933"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048704"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hexadecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41916"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9652"},{"database":"Rhea","id":"RHEA-COMP:9653"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78483"},{"database":"ChEBI","id":"CHEBI:78487"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048051"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41917"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048051"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41836"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9632"},{"database":"Rhea","id":"RHEA-COMP:9633"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78459"},{"database":"ChEBI","id":"CHEBI:78460"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047394"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41837"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047394"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41788"},{"database":"Rhea","id":"RHEA-COMP:9621"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78446"}],"ecNumber":"2.3.1.38","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048691"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41789"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048691"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41852"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9636"},{"database":"Rhea","id":"RHEA-COMP:9637"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78463"},{"database":"ChEBI","id":"CHEBI:78464"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049533"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41853"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049533"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:30123"},{"database":"Rhea","id":"RHEA-COMP:9648"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30807"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78477"}],"ecNumber":"3.1.2.14","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048289"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:30124"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048289"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"tetradecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41900"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9648"},{"database":"Rhea","id":"RHEA-COMP:9649"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78477"},{"database":"ChEBI","id":"CHEBI:78478"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047451"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:41901"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047451"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"pantetheine 4'-phosphate","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:47942"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005189"}],"value":"Lipid metabolism"}],"commentType":"PATHWAY"}],"features":[{"type":"Domain","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":455,"modifier":"EXACT"}},"description":"Ketosynthase family 3 (KS3)","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52004"}]},{"type":"Domain","location":{"start":{"value":898,"modifier":"EXACT"},"end":{"value":1165,"modifier":"EXACT"}},"description":"PKS/mFAS DH","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52019"}]},{"type":"Domain","location":{"start":{"value":1920,"modifier":"EXACT"},"end":{"value":1998,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":2024,"modifier":"EXACT"},"end":{"value":2448,"modifier":"EXACT"}},"description":"Ketosynthase family 3 (KS3)","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52004"}]},{"type":"Domain","location":{"start":{"value":2912,"modifier":"EXACT"},"end":{"value":3183,"modifier":"EXACT"}},"description":"PKS/mFAS DH","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52019"}]},{"type":"Domain","location":{"start":{"value":3618,"modifier":"EXACT"},"end":{"value":3696,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":3716,"modifier":"EXACT"},"end":{"value":4141,"modifier":"EXACT"}},"description":"Ketosynthase family 3 (KS3)","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52004"}]},{"type":"Domain","location":{"start":{"value":4608,"modifier":"EXACT"},"end":{"value":4875,"modifier":"EXACT"}},"description":"PKS/mFAS DH","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52019"}]},{"type":"Domain","location":{"start":{"value":5312,"modifier":"EXACT"},"end":{"value":5390,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Region","location":{"start":{"value":898,"modifier":"EXACT"},"end":{"value":1017,"modifier":"EXACT"}},"description":"N-terminal hotdog fold","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Region","location":{"start":{"value":1029,"modifier":"EXACT"},"end":{"value":1165,"modifier":"EXACT"}},"description":"C-terminal hotdog fold","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Region","location":{"start":{"value":1904,"modifier":"EXACT"},"end":{"value":1923,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":2912,"modifier":"EXACT"},"end":{"value":3031,"modifier":"EXACT"}},"description":"N-terminal hotdog fold","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Region","location":{"start":{"value":3043,"modifier":"EXACT"},"end":{"value":3183,"modifier":"EXACT"}},"description":"C-terminal hotdog fold","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Region","location":{"start":{"value":4608,"modifier":"EXACT"},"end":{"value":4727,"modifier":"EXACT"}},"description":"N-terminal hotdog fold","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Region","location":{"start":{"value":4739,"modifier":"EXACT"},"end":{"value":4875,"modifier":"EXACT"}},"description":"C-terminal hotdog fold","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Region","location":{"start":{"value":5400,"modifier":"EXACT"},"end":{"value":5430,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1904,"modifier":"EXACT"},"end":{"value":1917,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":5409,"modifier":"EXACT"},"end":{"value":5427,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10022"}]},{"type":"Active site","location":{"start":{"value":930,"modifier":"EXACT"},"end":{"value":930,"modifier":"EXACT"}},"description":"Proton acceptor; for dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Active site","location":{"start":{"value":1090,"modifier":"EXACT"},"end":{"value":1090,"modifier":"EXACT"}},"description":"Proton donor; for dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Active site","location":{"start":{"value":2194,"modifier":"EXACT"},"end":{"value":2194,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10022"}]},{"type":"Active site","location":{"start":{"value":2944,"modifier":"EXACT"},"end":{"value":2944,"modifier":"EXACT"}},"description":"Proton acceptor; for dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Active site","location":{"start":{"value":3104,"modifier":"EXACT"},"end":{"value":3104,"modifier":"EXACT"}},"description":"Proton donor; for dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Active site","location":{"start":{"value":3887,"modifier":"EXACT"},"end":{"value":3887,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10022"}]},{"type":"Active site","location":{"start":{"value":4640,"modifier":"EXACT"},"end":{"value":4640,"modifier":"EXACT"}},"description":"Proton acceptor; for dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Active site","location":{"start":{"value":4800,"modifier":"EXACT"},"end":{"value":4800,"modifier":"EXACT"}},"description":"Proton donor; for dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Modified residue","location":{"start":{"value":1958,"modifier":"EXACT"},"end":{"value":1958,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]},{"type":"Modified residue","location":{"start":{"value":3656,"modifier":"EXACT"},"end":{"value":3656,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]},{"type":"Modified residue","location":{"start":{"value":5350,"modifier":"EXACT"},"end":{"value":5350,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022450"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}],"id":"KW-0596","category":"PTM","name":"Phosphopantetheine"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022553"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022898"}],"id":"KW-0663","category":"Ligand","name":"Pyridoxal phosphate"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022799"}],"id":"KW-0702","category":"PTM","name":"S-nitrosylation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52546.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52546.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD52546.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237382652.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"KEGG","id":"scor:J3U87_08740","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAHWGRN","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0019171","properties":[{"key":"GoTerm","value":"F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004316","properties":[{"key":"GoTerm","value":"F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004315","properties":[{"key":"GoTerm","value":"F:3-oxoacyl-[acyl-carrier-protein] synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004313","properties":[{"key":"GoTerm","value":"F:[acyl-carrier-protein] S-acetyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0141148","properties":[{"key":"GoTerm","value":"F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004312","properties":[{"key":"GoTerm","value":"F:fatty acid synthase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0016297","properties":[{"key":"GoTerm","value":"F:fatty acyl-[ACP] hydrolase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0031177","properties":[{"key":"GoTerm","value":"F:phosphopantetheine binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006633","properties":[{"key":"GoTerm","value":"P:fatty acid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd05195","properties":[{"key":"EntryName","value":"enoyl_red"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd08956","properties":[{"key":"EntryName","value":"KR_3_FAS_SDR_x"},{"key":"MatchStatus","value":"3"}]},{"database":"CDD","id":"cd00833","properties":[{"key":"EntryName","value":"PKS"},{"key":"MatchStatus","value":"3"}]},{"database":"FunFam","id":"3.40.50.720:FF:000209","properties":[{"key":"EntryName","value":"Polyketide synthase Pks12"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.47.10:FF:000019","properties":[{"key":"EntryName","value":"Polyketide synthase type I"},{"key":"MatchStatus","value":"3"}]},{"database":"FunFam","id":"3.40.366.10:FF:000002","properties":[{"key":"EntryName","value":"Probable polyketide synthase 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.3290","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"3"}]},{"database":"Gene3D","id":"3.40.47.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"3"}]},{"database":"Gene3D","id":"3.40.50.11460","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.1200.10","properties":[{"key":"EntryName","value":"ACP-like"},{"key":"MatchStatus","value":"3"}]},{"database":"Gene3D","id":"3.40.50.1820","properties":[{"key":"EntryName","value":"alpha/beta hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.366.10","properties":[{"key":"EntryName","value":"Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"},{"key":"MatchStatus","value":"3"}]},{"database":"Gene3D","id":"3.90.180.10","properties":[{"key":"EntryName","value":"Medium-chain alcohol dehydrogenases, catalytic domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"3"}]},{"database":"Gene3D","id":"3.10.129.110","properties":[{"key":"EntryName","value":"Polyketide synthase dehydratase"},{"key":"MatchStatus","value":"3"}]},{"database":"InterPro","id":"IPR029058","properties":[{"key":"EntryName","value":"AB_hydrolase_fold"}]},{"database":"InterPro","id":"IPR001227","properties":[{"key":"EntryName","value":"Ac_transferase_dom_sf"}]},{"database":"InterPro","id":"IPR036736","properties":[{"key":"EntryName","value":"ACP-like_sf"}]},{"database":"InterPro","id":"IPR014043","properties":[{"key":"EntryName","value":"Acyl_transferase_dom"}]},{"database":"InterPro","id":"IPR016035","properties":[{"key":"EntryName","value":"Acyl_Trfase/lysoPLipase"}]},{"database":"InterPro","id":"IPR013154","properties":[{"key":"EntryName","value":"ADH-like_N"}]},{"database":"InterPro","id":"IPR020843","properties":[{"key":"EntryName","value":"ER"}]},{"database":"InterPro","id":"IPR011032","properties":[{"key":"EntryName","value":"GroES-like_sf"}]},{"database":"InterPro","id":"IPR014031","properties":[{"key":"EntryName","value":"KAS_C"}]},{"database":"InterPro","id":"IPR014030","properties":[{"key":"EntryName","value":"KAS_N"}]},{"database":"InterPro","id":"IPR018201","properties":[{"key":"EntryName","value":"Ketoacyl_synth_AS"}]},{"database":"InterPro","id":"IPR057326","properties":[{"key":"EntryName","value":"KR_dom"}]},{"database":"InterPro","id":"IPR016036","properties":[{"key":"EntryName","value":"Malonyl_transacylase_ACP-bd"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"InterPro","id":"IPR032821","properties":[{"key":"EntryName","value":"PKS_assoc"}]},{"database":"InterPro","id":"IPR020841","properties":[{"key":"EntryName","value":"PKS_Beta-ketoAc_synthase_dom"}]},{"database":"InterPro","id":"IPR042104","properties":[{"key":"EntryName","value":"PKS_dehydratase_sf"}]},{"database":"InterPro","id":"IPR020807","properties":[{"key":"EntryName","value":"PKS_DH"}]},{"database":"InterPro","id":"IPR049551","properties":[{"key":"EntryName","value":"PKS_DH_C"}]},{"database":"InterPro","id":"IPR049552","properties":[{"key":"EntryName","value":"PKS_DH_N"}]},{"database":"InterPro","id":"IPR013968","properties":[{"key":"EntryName","value":"PKS_KR"}]},{"database":"InterPro","id":"IPR049900","properties":[{"key":"EntryName","value":"PKS_mFAS_DH"}]},{"database":"InterPro","id":"IPR050091","properties":[{"key":"EntryName","value":"PKS_NRPS_Biosynth_Enz"}]},{"database":"InterPro","id":"IPR020806","properties":[{"key":"EntryName","value":"PKS_PP-bd"}]},{"database":"InterPro","id":"IPR009081","properties":[{"key":"EntryName","value":"PP-bd_ACP"}]},{"database":"InterPro","id":"IPR006162","properties":[{"key":"EntryName","value":"Ppantetheine_attach_site"}]},{"database":"InterPro","id":"IPR055123","properties":[{"key":"EntryName","value":"SpnB-like_Rossmann"}]},{"database":"InterPro","id":"IPR020802","properties":[{"key":"EntryName","value":"TesA-like"}]},{"database":"InterPro","id":"IPR001031","properties":[{"key":"EntryName","value":"Thioesterase"}]},{"database":"InterPro","id":"IPR016039","properties":[{"key":"EntryName","value":"Thiolase-like"}]},{"database":"PANTHER","id":"PTHR43775","properties":[{"key":"EntryName","value":"FATTY ACID SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43775:SF51","properties":[{"key":"EntryName","value":"INACTIVE PHENOLPHTHIOCEROL SYNTHESIS POLYKETIDE SYNTHASE TYPE I PKS1-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00698","properties":[{"key":"EntryName","value":"Acyl_transf_1"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF08240","properties":[{"key":"EntryName","value":"ADH_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13602","properties":[{"key":"EntryName","value":"ADH_zinc_N_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22621","properties":[{"key":"EntryName","value":"CurL-like_PKS_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16197","properties":[{"key":"EntryName","value":"KAsynt_C_assoc"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF00109","properties":[{"key":"EntryName","value":"ketoacyl-synt"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF02801","properties":[{"key":"EntryName","value":"Ketoacyl-synt_C"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF08659","properties":[{"key":"EntryName","value":"KR"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF21089","properties":[{"key":"EntryName","value":"PKS_DH_N"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF00550","properties":[{"key":"EntryName","value":"PP-binding"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF14765","properties":[{"key":"EntryName","value":"PS-DH"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF22953","properties":[{"key":"EntryName","value":"SpnB_Rossmann"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF00975","properties":[{"key":"EntryName","value":"Thioesterase"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00827","properties":[{"key":"EntryName","value":"PKS_AT"},{"key":"MatchStatus","value":"3"}]},{"database":"SMART","id":"SM00826","properties":[{"key":"EntryName","value":"PKS_DH"},{"key":"MatchStatus","value":"3"}]},{"database":"SMART","id":"SM00829","properties":[{"key":"EntryName","value":"PKS_ER"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00822","properties":[{"key":"EntryName","value":"PKS_KR"},{"key":"MatchStatus","value":"3"}]},{"database":"SMART","id":"SM00825","properties":[{"key":"EntryName","value":"PKS_KS"},{"key":"MatchStatus","value":"3"}]},{"database":"SMART","id":"SM00823","properties":[{"key":"EntryName","value":"PKS_PP"},{"key":"MatchStatus","value":"3"}]},{"database":"SMART","id":"SM01294","properties":[{"key":"EntryName","value":"PKS_PP_betabranch"},{"key":"MatchStatus","value":"3"}]},{"database":"SMART","id":"SM00824","properties":[{"key":"EntryName","value":"PKS_TE"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF47336","properties":[{"key":"EntryName","value":"ACP-like"},{"key":"MatchStatus","value":"3"}]},{"database":"SUPFAM","id":"SSF53474","properties":[{"key":"EntryName","value":"alpha/beta-Hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52151","properties":[{"key":"EntryName","value":"FabD/lysophospholipase-like"},{"key":"MatchStatus","value":"3"}]},{"database":"SUPFAM","id":"SSF50129","properties":[{"key":"EntryName","value":"GroES-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"7"}]},{"database":"SUPFAM","id":"SSF55048","properties":[{"key":"EntryName","value":"Probable ACP-binding domain of malonyl-CoA ACP transacylase"},{"key":"MatchStatus","value":"3"}]},{"database":"SUPFAM","id":"SSF53901","properties":[{"key":"EntryName","value":"Thiolase-like"},{"key":"MatchStatus","value":"3"}]},{"database":"PROSITE","id":"PS50075","properties":[{"key":"EntryName","value":"CARRIER"},{"key":"MatchStatus","value":"3"}]},{"database":"PROSITE","id":"PS00606","properties":[{"key":"EntryName","value":"KS3_1"},{"key":"MatchStatus","value":"3"}]},{"database":"PROSITE","id":"PS52004","properties":[{"key":"EntryName","value":"KS3_2"},{"key":"MatchStatus","value":"3"}]},{"database":"PROSITE","id":"PS00012","properties":[{"key":"EntryName","value":"PHOSPHOPANTETHEINE"},{"key":"MatchStatus","value":"3"}]},{"database":"PROSITE","id":"PS52019","properties":[{"key":"EntryName","value":"PKS_MFAS_DH"},{"key":"MatchStatus","value":"3"}]}],"sequence":{"value":"MTEPTRLQKAMAAIKKLRGRIDELENSRPEPIAIVSMACRLPGGIAAPEDFWSLLVEGRDAIGPLPADRWDAESLYDPDPDAAGKIFAKEGGFIDEADRFDAAFFGIAPREAQAMDPQQRLLLEVTWEALERANMVPAELSGSKTGVFVGAMSSDYMMGAGSGLESVDGYTGTGTSGAVVAGRLSYFLGLQGPALTVDTACSSSLVAVHLAAAALRDGECDMALAGGVTVMNSPAIFISFSRLRAMAGDGRCKSFSANADGTGWAEGCGVLVLKRLADARRDGDRILALVRGSAVNQDGRSQGLTAPNGPSQQRVLRQALRSAGITPAELDVVEAHGTGTRLGDPLEAEALAAVFGASRAPQQPLYLGSAKSHIGHAQAAAGVAGIIKMVLALQHDLLPANLHADQPSPDIRWRESGLSLLQQQIPWPRRDTRLRRAGISSFGISGTNAHVILEEAPVPSQTTTPNQEPAHTPAPDTLLLLSSRTEAALSGQVQRFHTFMDGKSAAERADICHTAANHRTHFPYRCWSAWRDGNLRAQRSGSVGGTPRVALLFTGQGSQLPAMAAPLYQREPVFRETVDRCLALTGPLPLLTDDASVHLTEKTQPCLFVFEYALYRLWSSWGLKPVAVLGHSIGELVAATVAGVFTLEEGLCLAVARGRLMGAATGDGSMASLEADETTARRLIAPFGERLAIAGLNSPSQTLISGETAAIDEFLAGLDIRAKRLETSHAFHSAQMDSALDPFEQLVAETRPKAPQLMIASNLSGGLVSDEMCAPAYWARQIREPVRFIDGVRTLIQHGCNTFLELGPQPILCGLGAACTEVDCSWLASAKKNEPHTQTMLEAAGGLFLRGVTLDWKRVVPGHATEIPTYAFDRQSYRLRGAETATDVTAAGQEAPHHPLLGAVLEQADGSATLFTARLSPRRTPWLSDHRIYETPLFPGTGFLELAWAAARRLEKGGVEELNLAAPLVLVDSGAVQLQLRVDDDGGFSIHARPEAEDRTDWVLHAVGRLHDKQPPQETETWSWPPSNAERLQTDGLYDSLQAMGFDYGPAFRGLREVWRRENMLFARVAVAEQEVELAEGHGLFPPLLDAVLHSLLCGEETRDEVQLPFAWRDACLWAHGAREMVARLVPRADGTEIKLFDVSGNPLGRIGLLATRVARPDQIRQAVRREPRDLYRVNWRAIPLPADSTSAREPVIFECPCEGSGTGADMVIVNQVLAQLQRALADEALQGRSLVWLTRNAVCAGPDDPVSRLDQAPVWGLLRAARQEHPERDLRLIDLDDQSDKASLEAALSIVDEPELALRFGKALAPRVLSASRARTNLEAPADTPLVRLRVRERGSLDSLQLVAHEADSRPLQDGEVRIAIRAVGLNFRDVLGALDMLPNPSEPGLEGAGIVLEVGADVSRFQPGDRVFGLLESSAATHVVADQAVLAKIPAGLSFVEAATIPTTFVTAWYALVDLGRLRAGERVLIHAAAGGTGQAAVALARHLGAEVFATAGPAKWPMLLKQGLTADHLASSRDTKFETAFREVTSGRGVDLVLNSLTDEKIDASLRLLAKGGRFLELGVTDTRSPAEIETAFPGVRYLPFLLMDAGNRRLGEILRELCPLFETGSLAPLHHTAYDLREAPAAFRFMAQARHRGKLVMTLPPSLETKSDREVLITGGTGELGSRLAVHLVREYGVTDLLLTSRRGAAAPGAAELTAELEALGARVRLVACDVSDAASLRAAVDFERVRAVFHCAGVLDDGLLTQMTPESLQRTWQPKAAAAWHLHEQTCRHDLDCFVMFSSVAGVLGGPGQSNYAAANSFLDALAAHRRKLGLPALSLAWGLWEQRGFGMTAHLGAADLNRMRRSGFGALSIERGLHLLDLALTRPDTLLVPMAADLARKRREEVAPILRELARANDPPRAAAAASAAAPRPRDAEGMLQATRAEIASALALPGPESVPSDQPLSEMGLDSLMAVEIRNRLNRLVDSPLPTTLLFDYPNARALATFIQETQTPANTGNQAEVVPSEAAPIDPESRLDEPIAVVAVACRLPGDVTSPEAYWSLLERGSDAVGPFPERWDTEALYDPDPDAAGKSYAKEGGFLRDVEGFDAAFFGISPREARGMDPQQRILLEVTWEALERARLMPATLVESRTGVYIGAMNSDYMEPDAGREDLDGYAGTGTSSAVVAGRLSYFLGLQGPAMTVDTACSSSLVALHLAVQGLRLGECDAALAGGVTVMNTPSIFVEFSRLKGMAADGRCKSFSARADGAGWAEGCGMLVLKRLSDAQRDGDRVLALVRGTAVNQDGRSQGLTAPNGPSQQRVIRQALHTAGLAPNDLDAIEAHGTGTRLGDPLEAGALTAVFGSNRDEARPLWLGSSKSNIGHAQAAAGVAGIIKMVLALQHEQLPKTLYVEEPSPHIDWQGSGLTLLKEQQPWPRDEARPRRAGVSSFGVSGTNAHIVLEEAPADTAPAPEPESQSKPADHLLLLSAMTPAALREQAARYRLLASAEPAIDPADLCFSAAVSRTAFSHRSALAFSDREQLLEGLHQVDGHDAVTDPRVVFVYPGQGAQWLGMGRQLLERSEPFRTALEACDTLIRAEAGFSVIDELRAAPERSRMREIQVVQPLLFSVAAGLTAVWRAHGVEADAVVGHSQGEVAAAYAAGHLSLEDAVAVICRRSRLLERIAGQGAMALLEMSREDTERMLQQRRYGDRLSVAVVNSDKGCVVSGETAAIDELVAEMQRQSLFCRKIQVDVASHSPAVDALRDDLIGELDQLSPRAGNIPMWSTVKAEPIAGTDLNAAYWYDNLRQPVLYGKVVDQLWQSGPHVFVEISPHPLLTVATEEVRAAVGGSGGTVSSLRRDQPEVKTLLHGLGQAWCRGVSVAWPEILPGRLIDLPTYPFQHKPYRRESAAKQTADVRAAGLSAAEHPLLGAETPLAEDGDFLFTARISPRRLPWLRDHAVHGTVLFPGTGFLELAWAAARRLEKAAVEELTLAQPLVLHAERGVQLQLRVDATGGFAIHGRPEDDDQAPWVPHALGRLSAAAPQPEPERLSWPPAGREAVAVDTLYDELSELGFAYGPAFRNLREVWRDERALYAKVVLPERHAGEAEAYGMFPALLDAALHALYYVAHVNDEGPPQVRLPFHWQGASLWAGGARELLARLEPDTAGARIALYDGGGNPLGTIRQLITRPATAEQVRAALRREARHLYRLNWREIALSQTPDDNTQRALIFKCAQAEGEGAATALIAAQTAAAQLSETTADETSRDRPLVWLTRNAVAVEPDETLTRPQQASIWGLLRSARGEYPDRDLRLIDCDADTPEAALRAALAVQGEPEMALRGQRLFVPRLSPVSNLEENATAEPQTPALRSRPDAAVLITGGTGELGRLVAERLVRDHAITHLILTSRRGSDAPGAASLRTALEALGARVHFVACDAADRDALAAAIDFSAIRGIVHCAGVLDDGLLAQMTPESLQRVWQPKAAGAWNLHCLTADHQLDFFILFSSAAGVLGGPGQGNYAAANSYLDALAGFRRAHGLPALSLAWGLWEQQGVGMTAHLGTADLARLARQGIGAFSVERGLEQLDAALKRPESLLIPMVLHLGRKSRDQVPALLRDMARPAPRRRAAAATAGQVMDADGMLHMVRGEIASVLALPGAGAIPADKPLHELGLDSLMAVEIRNRLSQAVDMDLPTTLLFDYANAGTLAEYLLSQRQPASASDARVTTRPRIQALDEPIAVISTACRLPGGITTPEAFWSLLDNGRDAIEPFPHDRWDIRALYDADPEAVGKTYAKEGGFLHDINGFDAAFFGISPREAQGMDPQQRLLLEVAWEALERAFIKPQDKSGNRTGVFIGAMNSDYRVMDVGLDGLDGYAGTGTSSAVIAGRLSYTLGLQGPAMTVDTACSSSLAALHLAVTSLRLGECDAALAGGVTVMNSPSIFVEFSRLKGMAPDGRCKSFSAWADGAGWAEGCGILVLKRLSDAHRDGDPILALVRGTAVNQDGRSQGLTAPNGPSQQRVIREALNAASVTPDDVDAVEAHGTGTRLGDPLEAGALSAVFGPGRPANRPLYLGSAKSNIGHTQAAAGAAGLIKMIEALQHEKLPRTLHTAQPNPDVNWSAGGLALLTEGREWKRDPARPRLVGISAFGISGTNAHVILEEAPASPPAAAAAMAPTAESEPSDNLLLLSAKSAAALRDQAARYRSWLEDHPTANLADICFTAAQARQHFEHRWALAFSTREQLLADLQRVGHIQAIAQPRIVFVYPGQGAQWLGMGRQLMAQSEPFAAALRECDELIREQAGFSVIEALHATAEHSRFAETHVIQPLLFAMYVGLTAVWRARGIEADAVVGHSQGEVAAAYAAGMLSLEDAVSVIYLRSLLMERISGQGAMALVEMDQHEVAQILDRDEYANRLSIAVVNSPKNIVVSGEAVAVDALVAELQQRSVFCRKVQTDFASHSPAVDALRDDLLRELDGLRPRAGNIPMWSTVSAEPIAGEQLTADYWFANMREPVQYGEVVRQLWQDGPHVFIEMSPHPLLTVATEAVRGEVGGSGGMVSSLRRDQPEQLMLLHGLGQAWCRGVAVPFSRLMTGRPTQVPTYPFQHEQFRLPLKPAGTTDVRAAGLGGSEHPLLGAVTALAEDGDILFSARLSAETHPWLRDHAVFNTVLFPGVAFLELVWAAAHRQNKPGIEELTLAQPLVLDGNQAVQLQLRLDDAGSFTIHGRPERETETAWMLHAMGRLSQTGVSPSTETLVWPPTDAEAVTLNDLYTDLAELGFEYGPAFQALSEVWRNGQTLYAKVVVDDQRESEAEAYGLFPALLDAALHALFCRGDAQREVRLPFSWRGAVLWAHGAEHLYARLEPASEGVRISLFDGGGNALGRIDQMITRPATAQQIRAALQREARHLYAPTWRAISLDQAQGAESADQGALVFTCSDAVGENAAAGLTQSRSVMDQLRSALLDESLKHRPLVWLTRRAVSVGPDDPVVRPQQAPIWGMLRSARAEHPGRDLRLIDCDETTPEALIQAALAVREEPELALRGSDALAPRLLPADRPVSRAEQTSETAPQPLAAPEGAVLITGGTGELGRLTAEWMVREMGVTRLVLTSRRGSSAPGATELATRLNGQGAVVDIVACDVADPASLQAAMDVADIRGIVHCAGVLADGLLTELKPDQLTRVWQSKAAGAWNLHRLTRDRPLDFFILFSSAAGVLGGPGQSNYAAANTYLDGLASWRRGQGLPALSLAWGLWERQGFGMTAALGQADMARMKRAGLRAFSPEAGLAQLAAAIGRPESLLVPVMLELGERRDRLPALLRELVPAARPRHAAPAAVEPVVDAESMARMVRGEIAAVLALPGAAAVTAEKPLRDLGLDSLMAVEIRNRLAQALAVDLPATLLFDHEHVADLIAWLVDRFLDTAAVQTSAGTTGPVELPGREKHRTDEIKSGTRSESAHANPSSWLHRALFEADETATVDLLLLAGRMRMNRQGADAGNAPVAMRLLADGPGQGALVCIPSMIPAQSLQYRPLAETLVGRLPVYCITHPGYQAQEPLFASLSSMATAYADHIGTFLSERPNTLLAHSAGGPAAIEVTRILERRGTPPAGLVLVDSLPPASTWSTALHPALMVRDVLARAVPVRESQPLTAALQHLSEAELTAFGWYTPFTFEPGHKPNTPTLFVSAAELMAVQQSQTCPDMLSRPSDYWKEHCPNLTEVTSPGDHFTMLTQYAAELSAHILEWRSRL","length":5717,"molWeight":614415,"crc64":"0665A02DF959D548","md5":"8ABF7A7A6F6D330985E827014044720B"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":39,"COFACTOR":1,"PATHWAY":1},"countByFeatureType":{"Domain":9,"Region":8,"Compositional bias":2,"Active site":9,"Modified residue":3},"uniParcId":"UPI001AF8898E"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TCW1","uniProtkbId":"A0A8A4TCW1_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":17,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47939.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"Probable dual-specificity RNA methyltransferase RlmN"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"2.1.1.192"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"23S rRNA (adenine(2503)-C(2))-methyltransferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"23S rRNA m2A2503 methyltransferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"Ribosomal RNA large subunit methyltransferase N"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"tRNA (adenine(37)-C(2))-methyltransferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"tRNA m2A37 methyltransferase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47939.1"}],"value":"rlmN"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47939.1"}],"value":"J3U87_20325"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42916"},{"database":"Rhea","id":"RHEA-COMP:10000"},{"database":"Rhea","id":"RHEA-COMP:10001"},{"database":"Rhea","id":"RHEA-COMP:10152"},{"database":"Rhea","id":"RHEA-COMP:10282"},{"database":"ChEBI","id":"CHEBI:17319"},{"database":"ChEBI","id":"CHEBI:33737"},{"database":"ChEBI","id":"CHEBI:33738"},{"database":"ChEBI","id":"CHEBI:57844"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:74411"},{"database":"ChEBI","id":"CHEBI:74497"}],"ecNumber":"2.1.1.192","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:43332"},{"database":"Rhea","id":"RHEA-COMP:10000"},{"database":"Rhea","id":"RHEA-COMP:10001"},{"database":"Rhea","id":"RHEA-COMP:10162"},{"database":"Rhea","id":"RHEA-COMP:10485"},{"database":"ChEBI","id":"CHEBI:17319"},{"database":"ChEBI","id":"CHEBI:33737"},{"database":"ChEBI","id":"CHEBI:33738"},{"database":"ChEBI","id":"CHEBI:57844"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:74411"},{"database":"ChEBI","id":"CHEBI:74497"}],"ecNumber":"2.1.1.192","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"[4Fe-4S] cluster","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49883"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007544"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"Belongs to the radical SAM superfamily. RlmN family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"Radical SAM core","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51918"}]},{"type":"Active site","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}]},{"type":"Active site","location":{"start":{"value":346,"modifier":"EXACT"},"end":{"value":346,"modifier":"EXACT"}},"description":"S-methylcysteine intermediate","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}]},{"type":"Binding site","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01266"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","note":"4Fe-4S-S-AdoMet"}},{"type":"Binding site","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":128,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01266"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","note":"4Fe-4S-S-AdoMet"}},{"type":"Binding site","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01266"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","note":"4Fe-4S-S-AdoMet"}},{"type":"Binding site","location":{"start":{"value":172,"modifier":"EXACT"},"end":{"value":173,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":204,"modifier":"EXACT"},"end":{"value":204,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":227,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":303,"modifier":"EXACT"},"end":{"value":303,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022485"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0004","category":"Ligand","name":"4Fe-4S"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023014"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022603"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022552"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0698","category":"Biological process","name":"rRNA processing"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022691"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022694"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01849"}],"id":"KW-0819","category":"Biological process","name":"tRNA processing"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47939.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47939.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47939.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A8A4TCW1","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_20325","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AACQEYT","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0051539","properties":[{"key":"GoTerm","value":"F:4 iron, 4 sulfur cluster binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0070040","properties":[{"key":"GoTerm","value":"F:rRNA (adenine(2503)-C2-)-methyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019843","properties":[{"key":"GoTerm","value":"F:rRNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0002935","properties":[{"key":"GoTerm","value":"F:tRNA (adenine(37)-C2)-methyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000049","properties":[{"key":"GoTerm","value":"F:tRNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0070475","properties":[{"key":"GoTerm","value":"P:rRNA base methylation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0030488","properties":[{"key":"GoTerm","value":"P:tRNA methylation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd01335","properties":[{"key":"EntryName","value":"Radical_SAM"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.150.530","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01849","properties":[{"key":"EntryName","value":"RNA_methyltr_RlmN"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR040072","properties":[{"key":"EntryName","value":"Methyltransferase_A"}]},{"database":"InterPro","id":"IPR048641","properties":[{"key":"EntryName","value":"RlmN_N"}]},{"database":"InterPro","id":"IPR027492","properties":[{"key":"EntryName","value":"RNA_MTrfase_RlmN"}]},{"database":"InterPro","id":"IPR004383","properties":[{"key":"EntryName","value":"rRNA_lsu_MTrfase_RlmN/Cfr"}]},{"database":"InterPro","id":"IPR007197","properties":[{"key":"EntryName","value":"rSAM"}]},{"database":"InterPro","id":"IPR058240","properties":[{"key":"EntryName","value":"rSAM_sf"}]},{"database":"NCBIfam","id":"TIGR00048","properties":[{"key":"EntryName","value":"rRNA_mod_RlmN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30544","properties":[{"key":"EntryName","value":"23S RRNA METHYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30544:SF5","properties":[{"key":"EntryName","value":"RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04055","properties":[{"key":"EntryName","value":"Radical_SAM"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21016","properties":[{"key":"EntryName","value":"RlmN_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF006004","properties":[{"key":"EntryName","value":"CHP00048"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDF00275","properties":[{"key":"EntryName","value":"adenosine_C2_methyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01062","properties":[{"key":"EntryName","value":"methyltransferase_(Class_A)"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00029","properties":[{"key":"EntryName","value":"Radical_SAM"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF102114","properties":[{"key":"EntryName","value":"Radical SAM enzymes"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51918","properties":[{"key":"EntryName","value":"RADICAL_SAM"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTATKVTPAKTTSNKENLFGKSAKEIRQRLQAIGEPAYREKQIRDWIYVKRVLDFAGMTNLSKALRAKLADAFVIEPPATYHKHQSKDGSVKYVFHLEAGGEVEAVYMPMTKGVTMCLSSQVGCAQACSFCMTAQMGFIRNLSPGEIVGQAFAIAHDQQLEDPFNIVMMGMGEPLLNLNNLMKAIDLFTDDLGFGLSPRRITVSTSGHVRNLLKLGQYPKLPRIAVSLNASTDEQRNQLMPVNLRWNIAELIDACKQLPIPPRERVTLEYVLIKDLNDTPADAKRLVRLVANLRCKINLIPFNETEGLSYRQPSLQVIEAFQAILNRSQIRNTVRWSKGRDIGAACGQLATPITKRGADLRLKSGAPSRKAGSAG","length":375,"molWeight":41510,"crc64":"5E8F7211BD860F34","md5":"07167C5517C4CD48FFD27710A32B6BA8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Active site":2,"Binding site":7},"uniParcId":"UPI001AF4014F"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TES8","uniProtkbId":"A0A8A4TES8_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":18,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48047.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"6.3.2.13"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Meso-A2pm-adding enzyme"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Meso-diaminopimelate-adding enzyme"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"UDP-MurNAc-tripeptide synthetase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"UDP-N-acetylmuramyl-tripeptide synthetase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"murE"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48047.1"}],"value":"J3U87_20885"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminopimelate + ATP = UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelate + ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23676"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57791"},{"database":"ChEBI","id":"CHEBI:83900"},{"database":"ChEBI","id":"CHEBI:83905"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"6.3.2.13","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004135"}],"value":"Cell wall biogenesis; peptidoglycan biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004135"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005898"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Belongs to the MurCDEF family. MurE subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"Mur ligase N-terminal catalytic","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01225"}]},{"type":"Domain","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":307,"modifier":"EXACT"}},"description":"Mur ligase central","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF08245"}]},{"type":"Domain","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":457,"modifier":"EXACT"}},"description":"Mur ligase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02875"}]},{"type":"Region","location":{"start":{"value":489,"modifier":"EXACT"},"end":{"value":524,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":405,"modifier":"EXACT"}},"description":"Meso-diaminopimelate recognition motif","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}]},{"type":"Compositional bias","location":{"start":{"value":489,"modifier":"EXACT"},"end":{"value":501,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":506,"modifier":"EXACT"},"end":{"value":515,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:83900"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate","id":"ChEBI:CHEBI:83900"}},{"type":"Binding site","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:83900"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate","id":"ChEBI:CHEBI:83900"}},{"type":"Binding site","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":178,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:83900"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate","id":"ChEBI:CHEBI:83900"}},{"type":"Binding site","location":{"start":{"value":186,"modifier":"EXACT"},"end":{"value":186,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:83900"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate","id":"ChEBI:CHEBI:83900"}},{"type":"Binding site","location":{"start":{"value":378,"modifier":"EXACT"},"end":{"value":378,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57791"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"meso-2,6-diaminopimelate","id":"ChEBI:CHEBI:57791"}},{"type":"Binding site","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":405,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57791"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"meso-2,6-diaminopimelate","id":"ChEBI:CHEBI:57791"}},{"type":"Binding site","location":{"start":{"value":455,"modifier":"EXACT"},"end":{"value":455,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57791"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"meso-2,6-diaminopimelate","id":"ChEBI:CHEBI:57791"}},{"type":"Binding site","location":{"start":{"value":459,"modifier":"EXACT"},"end":{"value":459,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57791"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"ligand":{"name":"meso-2,6-diaminopimelate","id":"ChEBI:CHEBI:57791"}},{"type":"Modified residue","location":{"start":{"value":218,"modifier":"EXACT"},"end":{"value":218,"modifier":"EXACT"}},"description":"N6-carboxylysine","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023306"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022618"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0132","category":"Biological process","name":"Cell division"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022960"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0133","category":"Biological process","name":"Cell shape"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023316"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022598"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022984"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00208"}],"id":"KW-0573","category":"Biological process","name":"Peptidoglycan synthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48047.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48047.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD48047.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377709.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TES8","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_20885","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAEXXSH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00219","properties":[{"key":"RectionId","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004326","properties":[{"key":"GoTerm","value":"F:tetrahydrofolylpolyglutamate synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008765","properties":[{"key":"GoTerm","value":"F:UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051301","properties":[{"key":"GoTerm","value":"P:cell division"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0009252","properties":[{"key":"GoTerm","value":"P:peptidoglycan biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008360","properties":[{"key":"GoTerm","value":"P:regulation of cell shape"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.90.190.20","properties":[{"key":"EntryName","value":"Mur ligase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1190.10","properties":[{"key":"EntryName","value":"Mur-like, catalytic domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1390.10","properties":[{"key":"EntryName","value":"MurE/MurF, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00208","properties":[{"key":"EntryName","value":"MurE"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR018109","properties":[{"key":"EntryName","value":"Folylpolyglutamate_synth_CS"}]},{"database":"InterPro","id":"IPR036565","properties":[{"key":"EntryName","value":"Mur-like_cat_sf"}]},{"database":"InterPro","id":"IPR004101","properties":[{"key":"EntryName","value":"Mur_ligase_C"}]},{"database":"InterPro","id":"IPR036615","properties":[{"key":"EntryName","value":"Mur_ligase_C_dom_sf"}]},{"database":"InterPro","id":"IPR013221","properties":[{"key":"EntryName","value":"Mur_ligase_cen"}]},{"database":"InterPro","id":"IPR000713","properties":[{"key":"EntryName","value":"Mur_ligase_N"}]},{"database":"InterPro","id":"IPR035911","properties":[{"key":"EntryName","value":"MurE/MurF_N"}]},{"database":"InterPro","id":"IPR005761","properties":[{"key":"EntryName","value":"UDP-N-AcMur-Glu-dNH2Pim_ligase"}]},{"database":"NCBIfam","id":"TIGR01085","properties":[{"key":"EntryName","value":"murE"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF001126","properties":[{"key":"EntryName","value":"PRK00139.1-4"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR23135","properties":[{"key":"EntryName","value":"MUR LIGASE FAMILY MEMBER"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR23135:SF4","properties":[{"key":"EntryName","value":"UDP-N-ACETYLMURAMOYL-L-ALANYL-D-GLUTAMATE--2,6-DIAMINOPIMELATE LIGASE MURE HOMOLOG, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01225","properties":[{"key":"EntryName","value":"Mur_ligase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02875","properties":[{"key":"EntryName","value":"Mur_ligase_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08245","properties":[{"key":"EntryName","value":"Mur_ligase_M"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53623","properties":[{"key":"EntryName","value":"MurD-like peptide ligases, catalytic domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53244","properties":[{"key":"EntryName","value":"MurD-like peptide ligases, peptide-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF63418","properties":[{"key":"EntryName","value":"MurE/MurF N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01011","properties":[{"key":"EntryName","value":"FOLYLPOLYGLU_SYNT_1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKLRELVDGIGELLAESSGDLKISQITHDSRKVQHGSMFVAIEGRHRDGHNYVGCATQLGAVCILTNQPEKVPFSIPICRVDDPRKAMALMARRLYDHPDQKIKIIGVTGTNGKTTTTYLTSSLLRRLGTCGRIGTLSYFNGHSEERATRTTPESSEFYRCLGEMVSNRCAYAAVEISSHGLMFDRVLGLELQYGIFTNLSRDHLDFHGNMESYFQAKHRQFSLLRRGGTAILNWDDPYAKRIVVPEGRHTIRFGQSEEADLRFEIGELGIKGASFRVNFKGDTHDFRIPLMGEHNVYNFTAALAVALCEGRKLSEIAQETGDIRAVPGRLELIDVGQTFGVAIDFAHTPDALQSVLRACAETNPKRMITVFGAGGDRDHSKRPEMGAIAGRYSDIVILTTDNPRTEDPEVIMDMVHKGIKRPLGPGFIRQTDRRQAIHAAIHMAEEGDLILIAGKGHETTQEIHGRHYPFSDWEVALGFIQQRIRAKPLREDRVDRDRTRPPATSPRNPASESATPGKGASDD","length":524,"molWeight":58283,"crc64":"39C76A88D1A9D4D1","md5":"2CF3DC047D5C193D9DFD9B61B2FDA181"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"PTM":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":3,"Region":1,"Motif":1,"Compositional bias":2,"Binding site":9,"Modified residue":1},"uniParcId":"UPI001AF683A6"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TFN7","uniProtkbId":"A0A8A4TFN7_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":23,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48370.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00073623"}],"value":"Phenolphthiocerol/phthiocerol polyketide synthase subunit E"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00066974"}],"value":"2.3.1.292"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00066651"}],"value":"6.2.1.69"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00078169"}],"value":"(Phenol)carboxyphthiodiolenone synthase subunit E"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00084020"}],"value":"Beta-ketoacyl-acyl-carrier-protein synthase I"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00079103"}],"value":"L-cysteine--[L-cysteinyl-carrier protein] ligase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00075053"}],"value":"Phthiocerol synthesis polyketide synthase type I PpsE"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48370.1"}],"value":"J3U87_22550"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00058455"}],"value":"Part of the PpsABCDE complex involved in the biosynthesis of the lipid core common to phthiocerols and phenolphthiocerols by successive additions of malonyl-CoA or methylmalonyl-CoA extender units. PpsA can accept as substrate the activated forms of either icosanoyl (C20), docosanoyl (C22) or lignoceroyl (C24) groups from FadD26, or a (4-hydroxyphenyl)-C17 or (4-hydroxyphenyl)-C19 fatty acyl from FadD29. PpsA initiates the biosynthesis and extends its substrate using a malonyl-CoA extender unit. The PpsB and PpsC proteins add the second and third malonyl-CoA extender units. PpsD adds an (R)-methylmalonyl unit and PpsE adds a second (R)-methylmalonyl unit. The incorporation of the methylmalonyl units results in formation of two branched methyl groups in the elongated product"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"17-(4-hydroxyphenyl)heptadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 2 (S)-methylmalonyl-CoA + 3 malonyl-CoA + 5 NADPH + 10 H(+) = C35-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 NADP(+) + 5 CoA + 2 H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:57756"},{"database":"Rhea","id":"RHEA-COMP:14272"},{"database":"Rhea","id":"RHEA-COMP:14989"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57327"},{"database":"ChEBI","id":"CHEBI:57384"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:133300"},{"database":"ChEBI","id":"CHEBI:142259"}],"ecNumber":"2.3.1.292","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00050973"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"19-(4-hydroxyphenyl)nonadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 2 (S)-methylmalonyl-CoA + 3 malonyl-CoA + 5 NADPH + 10 H(+) = C37-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 NADP(+) + 5 CoA + 2 H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:57760"},{"database":"Rhea","id":"RHEA-COMP:14273"},{"database":"Rhea","id":"RHEA-COMP:14990"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57327"},{"database":"ChEBI","id":"CHEBI:57384"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:133301"},{"database":"ChEBI","id":"CHEBI:142260"}],"ecNumber":"2.3.1.292","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051971"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22836"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"Rhea","id":"RHEA-COMP:9916"},{"database":"Rhea","id":"RHEA-COMP:14125"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78776"},{"database":"ChEBI","id":"CHEBI:138651"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10022"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"docosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 2 (S)-methylmalonyl-CoA + 3 malonyl-CoA + 5 NADPH + 10 H(+) = C34-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 NADP(+) + 5 CoA + 2 H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:57752"},{"database":"Rhea","id":"RHEA-COMP:14987"},{"database":"Rhea","id":"RHEA-COMP:14988"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57327"},{"database":"ChEBI","id":"CHEBI:57384"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:142237"},{"database":"ChEBI","id":"CHEBI:142238"}],"ecNumber":"2.3.1.292","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052119"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"holo-[peptidyl-carrier protein] + L-cysteine + ATP = L-cysteinyl-[peptidyl-carrier protein] + AMP + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:61680"},{"database":"Rhea","id":"RHEA-COMP:11480"},{"database":"Rhea","id":"RHEA-COMP:15906"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:35235"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:144926"},{"database":"ChEBI","id":"CHEBI:456215"}],"ecNumber":"6.2.1.69","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052643"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:61681"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052643"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"icosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 2 (S)-methylmalonyl-CoA + 3 malonyl-CoA + 5 NADPH + 10 H(+) = C32-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 NADP(+) + 5 CoA + 2 H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:57748"},{"database":"Rhea","id":"RHEA-COMP:14985"},{"database":"Rhea","id":"RHEA-COMP:14986"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57327"},{"database":"ChEBI","id":"CHEBI:57384"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:87848"},{"database":"ChEBI","id":"CHEBI:142236"}],"ecNumber":"2.3.1.292","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052745"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"NADP(+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001937"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:58349"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"pantetheine 4'-phosphate","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001957"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:47942"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004924"}],"value":"Siderophore biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00029443"}],"value":"In the C-terminal section; belongs to the NRP synthetase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":1040,"modifier":"EXACT"},"end":{"value":1115,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":2413,"modifier":"EXACT"},"end":{"value":2488,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":2511,"modifier":"EXACT"},"end":{"value":2935,"modifier":"EXACT"}},"description":"Ketosynthase family 3 (KS3)","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52004"}]},{"type":"Domain","location":{"start":{"value":3862,"modifier":"EXACT"},"end":{"value":4141,"modifier":"EXACT"}},"description":"PKS/mFAS DH","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52019"}]},{"type":"Domain","location":{"start":{"value":4218,"modifier":"EXACT"},"end":{"value":4293,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Region","location":{"start":{"value":2364,"modifier":"EXACT"},"end":{"value":2396,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":3862,"modifier":"EXACT"},"end":{"value":3987,"modifier":"EXACT"}},"description":"N-terminal hotdog fold","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Region","location":{"start":{"value":4001,"modifier":"EXACT"},"end":{"value":4141,"modifier":"EXACT"}},"description":"C-terminal hotdog fold","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Region","location":{"start":{"value":4189,"modifier":"EXACT"},"end":{"value":4214,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":2376,"modifier":"EXACT"},"end":{"value":2385,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":2386,"modifier":"EXACT"},"end":{"value":2396,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":4189,"modifier":"EXACT"},"end":{"value":4212,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":2684,"modifier":"EXACT"},"end":{"value":2684,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10022"}]},{"type":"Active site","location":{"start":{"value":3892,"modifier":"EXACT"},"end":{"value":3892,"modifier":"EXACT"}},"description":"Proton acceptor; for dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Active site","location":{"start":{"value":4064,"modifier":"EXACT"},"end":{"value":4064,"modifier":"EXACT"}},"description":"Proton donor; for dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01363"}]},{"type":"Modified residue","location":{"start":{"value":1075,"modifier":"EXACT"},"end":{"value":1075,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]},{"type":"Modified residue","location":{"start":{"value":2448,"modifier":"EXACT"},"end":{"value":2448,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]},{"type":"Modified residue","location":{"start":{"value":4253,"modifier":"EXACT"},"end":{"value":4253,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022832"}],"id":"KW-0276","category":"Biological process","name":"Fatty acid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022598"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023098"}],"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022450"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}],"id":"KW-0596","category":"PTM","name":"Phosphopantetheine"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022553"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022737"}],"id":"KW-0677","category":"Domain","name":"Repeat"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48370.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48370.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD48370.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237378024.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"KEGG","id":"scor:J3U87_22550","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ADGLNFR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0034081","properties":[{"key":"GoTerm","value":"C:polyketide synthase complex"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0004315","properties":[{"key":"GoTerm","value":"F:3-oxoacyl-[acyl-carrier-protein] synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0016491","properties":[{"key":"GoTerm","value":"F:oxidoreductase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0031177","properties":[{"key":"GoTerm","value":"F:phosphopantetheine binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0043041","properties":[{"key":"GoTerm","value":"P:amino acid activation for nonribosomal peptide biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006633","properties":[{"key":"GoTerm","value":"P:fatty acid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0044550","properties":[{"key":"GoTerm","value":"P:secondary metabolite biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd12116","properties":[{"key":"EntryName","value":"A_NRPS_Ta1_like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd12114","properties":[{"key":"EntryName","value":"A_NRPS_TlmIV_like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd19535","properties":[{"key":"EntryName","value":"Cyc_NRPS"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd08953","properties":[{"key":"EntryName","value":"KR_2_SDR_x"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd19531","properties":[{"key":"EntryName","value":"LCL_NRPS-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd02142","properties":[{"key":"EntryName","value":"McbC_SagB-like_oxidoreductase"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00833","properties":[{"key":"EntryName","value":"PKS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.1200.10:FF:000016","properties":[{"key":"EntryName","value":"Non-ribosomal peptide synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.559.10:FF:000023","properties":[{"key":"EntryName","value":"Non-ribosomal peptide synthetase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.980:FF:000001","properties":[{"key":"EntryName","value":"Non-ribosomal peptide synthetase"},{"key":"MatchStatus","value":"2"}]},{"database":"FunFam","id":"3.30.300.30:FF:000015","properties":[{"key":"EntryName","value":"Nonribosomal peptide synthase SidD"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.1200.10:FF:000005","properties":[{"key":"EntryName","value":"Nonribosomal peptide synthetase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.47.10:FF:000042","properties":[{"key":"EntryName","value":"Polyketide synthase Pks13"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.559.30:FF:000006","properties":[{"key":"EntryName","value":"Yersiniabactin polyketide/non-ribosomal peptide synthetase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.300.30","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"3"}]},{"database":"Gene3D","id":"3.30.70.3290","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.47.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.980","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"4"}]},{"database":"Gene3D","id":"1.10.1200.10","properties":[{"key":"EntryName","value":"ACP-like"},{"key":"MatchStatus","value":"3"}]},{"database":"Gene3D","id":"3.30.559.10","properties":[{"key":"EntryName","value":"Chloramphenicol acetyltransferase-like domain"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"2.30.38.10","properties":[{"key":"EntryName","value":"Luciferase, Domain 3"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.30.70.250","properties":[{"key":"EntryName","value":"Malonyl-CoA ACP transacylase, ACP-binding"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.366.10","properties":[{"key":"EntryName","value":"Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.109.10","properties":[{"key":"EntryName","value":"NADH Oxidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.10.1830","properties":[{"key":"EntryName","value":"Non-ribosomal peptide synthase, adenylation domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.559.30","properties":[{"key":"EntryName","value":"Nonribosomal peptide synthetase, condensation domain"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.10.129.110","properties":[{"key":"EntryName","value":"Polyketide synthase dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR010071","properties":[{"key":"EntryName","value":"AA_adenyl_dom"}]},{"database":"InterPro","id":"IPR001227","properties":[{"key":"EntryName","value":"Ac_transferase_dom_sf"}]},{"database":"InterPro","id":"IPR036736","properties":[{"key":"EntryName","value":"ACP-like_sf"}]},{"database":"InterPro","id":"IPR014043","properties":[{"key":"EntryName","value":"Acyl_transferase_dom"}]},{"database":"InterPro","id":"IPR016035","properties":[{"key":"EntryName","value":"Acyl_Trfase/lysoPLipase"}]},{"database":"InterPro","id":"IPR045851","properties":[{"key":"EntryName","value":"AMP-b_sf"}]},{"database":"InterPro","id":"IPR025110","properties":[{"key":"EntryName","value":"AMP-bd_C"}]},{"database":"InterPro","id":"IPR020845","properties":[{"key":"EntryName","value":"AMP-binding_CS"}]},{"database":"InterPro","id":"IPR000873","properties":[{"key":"EntryName","value":"AMP-dep_synth/lig_dom"}]},{"database":"InterPro","id":"IPR023213","properties":[{"key":"EntryName","value":"CAT-like_dom_sf"}]},{"database":"InterPro","id":"IPR001242","properties":[{"key":"EntryName","value":"Condensation_dom"}]},{"database":"InterPro","id":"IPR057737","properties":[{"key":"EntryName","value":"Condensation_MtbB-like"}]},{"database":"InterPro","id":"IPR014031","properties":[{"key":"EntryName","value":"KAS_C"}]},{"database":"InterPro","id":"IPR014030","properties":[{"key":"EntryName","value":"KAS_N"}]},{"database":"InterPro","id":"IPR018201","properties":[{"key":"EntryName","value":"Ketoacyl_synth_AS"}]},{"database":"InterPro","id":"IPR057326","properties":[{"key":"EntryName","value":"KR_dom"}]},{"database":"InterPro","id":"IPR016036","properties":[{"key":"EntryName","value":"Malonyl_transacylase_ACP-bd"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"InterPro","id":"IPR029479","properties":[{"key":"EntryName","value":"Nitroreductase"}]},{"database":"InterPro","id":"IPR000415","properties":[{"key":"EntryName","value":"Nitroreductase-like"}]},{"database":"InterPro","id":"IPR032821","properties":[{"key":"EntryName","value":"PKS_assoc"}]},{"database":"InterPro","id":"IPR020841","properties":[{"key":"EntryName","value":"PKS_Beta-ketoAc_synthase_dom"}]},{"database":"InterPro","id":"IPR042104","properties":[{"key":"EntryName","value":"PKS_dehydratase_sf"}]},{"database":"InterPro","id":"IPR020807","properties":[{"key":"EntryName","value":"PKS_DH"}]},{"database":"InterPro","id":"IPR049551","properties":[{"key":"EntryName","value":"PKS_DH_C"}]},{"database":"InterPro","id":"IPR049552","properties":[{"key":"EntryName","value":"PKS_DH_N"}]},{"database":"InterPro","id":"IPR013968","properties":[{"key":"EntryName","value":"PKS_KR"}]},{"database":"InterPro","id":"IPR049900","properties":[{"key":"EntryName","value":"PKS_mFAS_DH"}]},{"database":"InterPro","id":"IPR020806","properties":[{"key":"EntryName","value":"PKS_PP-bd"}]},{"database":"InterPro","id":"IPR009081","properties":[{"key":"EntryName","value":"PP-bd_ACP"}]},{"database":"InterPro","id":"IPR006162","properties":[{"key":"EntryName","value":"Ppantetheine_attach_site"}]},{"database":"InterPro","id":"IPR016039","properties":[{"key":"EntryName","value":"Thiolase-like"}]},{"database":"InterPro","id":"IPR041464","properties":[{"key":"EntryName","value":"TubC_N"}]},{"database":"InterPro","id":"IPR044894","properties":[{"key":"EntryName","value":"TubC_N_sf"}]},{"database":"NCBIfam","id":"TIGR01733","properties":[{"key":"EntryName","value":"AA-adenyl-dom"},{"key":"MatchStatus","value":"2"}]},{"database":"NCBIfam","id":"NF003417","properties":[{"key":"EntryName","value":"dltA"},{"key":"MatchStatus","value":"3"}]},{"database":"NCBIfam","id":"NF047870","properties":[{"key":"EntryName","value":"PKS_KR_start"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45527:SF1","properties":[{"key":"EntryName","value":"FATTY ACID SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45527","properties":[{"key":"EntryName","value":"NONRIBOSOMAL PEPTIDE SYNTHETASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00698","properties":[{"key":"EntryName","value":"Acyl_transf_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00501","properties":[{"key":"EntryName","value":"AMP-binding"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF13193","properties":[{"key":"EntryName","value":"AMP-binding_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00668","properties":[{"key":"EntryName","value":"Condensation"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF16197","properties":[{"key":"EntryName","value":"KAsynt_C_assoc"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00109","properties":[{"key":"EntryName","value":"ketoacyl-synt"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02801","properties":[{"key":"EntryName","value":"Ketoacyl-synt_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08659","properties":[{"key":"EntryName","value":"KR"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00881","properties":[{"key":"EntryName","value":"Nitroreductase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21089","properties":[{"key":"EntryName","value":"PKS_DH_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00550","properties":[{"key":"EntryName","value":"PP-binding"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF14765","properties":[{"key":"EntryName","value":"PS-DH"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18563","properties":[{"key":"EntryName","value":"TubC_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00827","properties":[{"key":"EntryName","value":"PKS_AT"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00826","properties":[{"key":"EntryName","value":"PKS_DH"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00822","properties":[{"key":"EntryName","value":"PKS_KR"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00825","properties":[{"key":"EntryName","value":"PKS_KS"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00823","properties":[{"key":"EntryName","value":"PKS_PP"},{"key":"MatchStatus","value":"3"}]},{"database":"SMART","id":"SM01294","properties":[{"key":"EntryName","value":"PKS_PP_betabranch"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56801","properties":[{"key":"EntryName","value":"Acetyl-CoA synthetase-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF47336","properties":[{"key":"EntryName","value":"ACP-like"},{"key":"MatchStatus","value":"3"}]},{"database":"SUPFAM","id":"SSF52777","properties":[{"key":"EntryName","value":"CoA-dependent acyltransferases"},{"key":"MatchStatus","value":"4"}]},{"database":"SUPFAM","id":"SSF52151","properties":[{"key":"EntryName","value":"FabD/lysophospholipase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55469","properties":[{"key":"EntryName","value":"FMN-dependent nitroreductase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF55048","properties":[{"key":"EntryName","value":"Probable ACP-binding domain of malonyl-CoA ACP transacylase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53901","properties":[{"key":"EntryName","value":"Thiolase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00455","properties":[{"key":"EntryName","value":"AMP_BINDING"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS50075","properties":[{"key":"EntryName","value":"CARRIER"},{"key":"MatchStatus","value":"3"}]},{"database":"PROSITE","id":"PS00606","properties":[{"key":"EntryName","value":"KS3_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52004","properties":[{"key":"EntryName","value":"KS3_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00012","properties":[{"key":"EntryName","value":"PHOSPHOPANTETHEINE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS52019","properties":[{"key":"EntryName","value":"PKS_MFAS_DH"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNSNDLLSLLVKAGIRLWIDGDKLRVDAPKGALTPELKRQLMAHKAELTAILEQARAQEARTSEPIPARDAAKPPVMSLAQQRLWLLDQLEGANSAYNLVFALRLEGKTDERALARALTRVVDRHESLRQRFAGDADERGACFRDVSTFRLEVRDLREEASPEEGLDALIQEETGHRFDLNRDWLVRTTMVRLQVQTGVLVVNLHHIVADGWSIGVFIRELADLYEAELRGTSSRLAEINTTYTDFSAWQRDVLEQPEAKEKLAAWVRQLEGGPACLQLPVAKERPLVPSYRGRREPFRFEPDAAKALARLCESSGATPFMVLEALFGLLLHRYGGQDRVHVGVPVANRVRPELEPLIGMFVNTLVLRHDFADGLNFREYLDRARHASLEAFDRAEIPFEQVVAALNPERNTSHSPIFQAMFILQNQPRVHHSVEGLTISQMALARTSAKFDLTMSITETEDVIAGSLEYNSDLFDLDTVQTLIRHFGNLLTSAAADPNRSITRLDMVDAEERLALLHGDNPEPSAVAPVAIHQLFERRAAETPDAIALAFGSGETGVALSYATLNRRANAVARNLMARGAGPGTRTAIFLDRSVDLVVAMLATLKAGSAYIPMDPEFPEKRLALMLEDAEPHAILTRADMEASLPDGPAIRLHVEGEAESDENPDVPLGENPTAYVIYTSGSTGRPKGVVIGHRAVVNFLESMGRRPGLGAEDVLLAVTTVSFDIAGLEIFLPLTRGARVVLASREEAGDGTALAAMIEHHQVSVMQATPATWRLLLAHDWTGSQNLKILCGGEALPPQLAEALLPKCGSLWNMYGPTEATIWSTCAEIRGETLTRGSIPLGQPIANTQCHILDAHLQPVPHGVAGQLYLGGAGLAEGYFKRPELTAEKFVAHPHAETLGLPADARLYATGDVVRRLPDGSLAFLGRVDHQVKLNGFRIELGDIEAALSRHPGVAQAVVLLNRDQTPARLVAYLVAEAGTELPQGSGPWRDFLRDEIQAYMIPSVYMILDAFPLTPNRKVDRKALPAPEAEVVKTPYVAPRNERERELARIWGEFLGRDEIGVHDNFFELGGHSLIAAKLVHAMNRAFETALPMRILFSHPTIAACAAEIGASPEGVVTDAERDGFTPTPLEPRPEARFEPFPLTDVQHAYWIGRSGLMTLGKVATHIYTELDSEILDPERCEHAIQTLVQRHDMLRAIVLETGEQQVLQKVPDYRIAVEDLRDLDEETARARLDAKRDRLSHQVLPADRWPLFHIEATRHGSGKIRFHVSMDALVTDGWSMRLLSKQFLELYADPGHEIEPLPLTFRDYVLAEHELEKSDLFAKSLQYWRERGPQLPAGPDLVLAVSPDELDEQRFERRSGSLEPERWQRLQARAQTLGVTPSALLLSAYAAILGTWSRAPRFTLNLTVFNRLPFHPDVDRLVGDFTSVLLLEVDQSQPATFAERARALQERLWLDWEHRFVSGVRVLREIARQNRETTKNMPVVFTSMLGMESKREATDLEADLDRVFTLTQTPQVWLDHQVAEQDGTLVYSWDAVSGLFPPGYLEDMFAAYQTLLDHLTDNDAIWSQKHLNLLPAHQRLVRERVNATEVALPGGLLHQGFLDQVARRGDAPAVIAPGRTLSYRELYREACQVARVLHDHGAQPNQLVAIVMEKGWEQAVAAIGVLLAGAAYMPVDAHWPEKRRLDLLETGEVSLVVTQEKCAEATAWPALVKTLVIGSEEHRAASETPPAVSNQHADLAYVIFTSGSTGKPKGVAIDHRGAVNTNQDIARRFGVGADDRVLALSALHFDLSVFDLFGMLAVGGAVVMPEAELAKDPSHWVQLMTHHDVTLWNTVPALMAMLAHHLEAHPESVPQTLRLAMMSGDWIPMDLPPSMQRLWSELTIYSLGGATEASIWSNFFKVTEIDPQWKSIPYGVPLANQSFTVLDHNLEPCPDWTPGDLYIGGIGLAQCYWGDPAKTAASFITHPVTGQRLYRTGDLGRYFPDGNLEFLGREDSQVKVNGYRVELGEIETALRAHPAIGQAVVAAHGKNRDNKRLVAYYTRQDGGVAASGEVIEDPVQRLAFKLAHHNFKRDFEALEELSLGAPDPETRHHYLERQSFRTYLGDRVTREDLAGLLRTLSPMTFGEGGRSKYRYPSAGSLFPVQTYIYVKEGRVTDVEAGFYYFHPETASLIRTSETRHMDTALFGPANLAASRDAAFGVFLVGKLNAIAPLYGDKARDFCILEAGHMGQLLMEQAPRFQLGLCPIGHLAFDAIADHLQVDGNHFCAYSFVGGAISEAQLGQVAGDDDLTRLQAMAAEAGIDLDTPAAAPSVATTAASSLPSVDDAELERALITFLQERLPEYMVPALFQRIDTIPLTPTGKLDRKALPDPGTSSARNSVQPTTAAAPVPRAETAAAPTTAVTEAASAVALNEVERKLAEIWKTHLELDHITAAENFFEIGGNSVMIVKVQNDINKAFDVDIRMVDMFRMPTLGALAEHVASLMKGEVVETSAAPIIPTVTSSQVRRQDIAIIGFDVRFPGAPDAEAFWQNLVDGVESVGPFTEDELLASGTTKAAMSHPRFVNAGSAIEESDLFDANFFGITPREAEQIDPQQRLLLECSYKALEHAGYAAEENRGPVGIFAGMSMNTYFIRNLLTNPKYFKTMNYFQALLNNDKDYLATRIAYRLNLRGPSLTVQTACSTSLVAIHLACQALMRDECDLALAGAVNVYNPPKTGYMYSEGLFLSADGHCRAFDHRATGTVFGSGLGSVVLKPLEKAQRDGDTVHAVIKGSAINNDGAQKVGYTAPSIQGQIDVVRRALENSGVHGEQIGYVEAHGTGTILGDPIEVEALHQAYGTDRNAQCGLGSVKTNLGHLETAAGVAGLIKTVLMLKHRKLVPTLNFEKPNPKIDFDSSPFFVSTELRDWEAKGSRYAGVSSFGIGGTNAHLILAEAPTREEAAAPPSHQLLTFSAKTEGALDRIGTDLADYLEAHPETDLADAAYTLHRGRGRFNHLGYAVVADRDQAIAALRGDGSAPASGRNANKNLAADIVFMFPGQGAQYLQMAADLYRDLPVFRQILSFNCSRMAPHMDRDPREILFPQAGKEDEARELLNQTTYTQPLLFLVEYSLAEVWKKWGLRPKAFIGHSVGELTAACQAGVLGFEDAVLMVLERGRLMQAMEPGDMVGVAASEAEILPLLDGKLALAAVNGPARCTVSGPADEIEAFVARCAERNLAARSLHTSHAFHGPMMDPMLDAFTDLVRTLDLHPPLTPFVSNLSGTWITDEQATDPAYWAAQLRGTVRFHEGLSTLMETNKRTLLEVGPGRTLATLANRHPSKTADHEVLASMRAIKSDDGDVAVLLGTLGRLWTLGATVDWAEFHFEQGRVRTPLPHYPFERERFWVEHDPSDPGSVEALLQGLTEGGTKTYVPVWKREPLIGASAGEAAGHHLILGDGAGLGAELAQRLSERGFQVSTVAAGDGFSRLEDRRFTIDMDDRANYGELFKSLKEGEGIAAVHVLTALDEEPDTSGDITVRFTRLLHLAQAIGEHAADAPLELNLITQGLWDVTGVEDLEPANALLLGPARVMRFEVPNVRCRVIDVVAPNGHRGARQRLVAQLCGELAKPIAEERAELALRGNHLWLRGLEYREIPETTSDPVHLVRGGVYLITGGLRGIGLALAGHLAQRYQARLVLLTRSAFPAEDTWDRWHAGSPTGDTVGALRHMIAAGGSVRVMRADVTDETRMREVIVAVREAFGDLNGVIHAAGLPGQGTIQLKEPTQSARVLAPKVMGARVLEKVTWDCNLDFTIYYGSVIGEMGGPGQVDYCAANAYLDALAHRRTNQDQPTFAIDWDGWSETGMAANARLGERQPAWDAVEPVTLPLFSARWDDGDQVTYYGSVDAERDWIVREHRVAGKPTIPGTAYVEMAASAFRHHSGLERLEIRDLLFLQPLSVADTGHANTGRADYRLVLQPNDSGYRFEVLSTDATGNRHQHAQGLVVPLETSSRVRFDLDSHRGAGAYETLDVADAKRAGEGRALVDVGPRWENVRWMGFGERDALVRQQLPEAFGEDLEAYTVHPAMLDVATGFAGLRLVNQHLPFTYKSIKISGRLPGELQTRAVRRSGEGELKEFDLVLCSASGQCLVEIEGYGLLEVRDRAGLQTSGGERPAALPPGLTNAAGLEAFDRIVNQGLTQVVVSSRDIHRVRATEAERADRRAGRGGAEDRHKRPELATEFTAPRNETERVLAEIWQELLGLERVGIEDDFLELGGDSLVATQMGNKVRAAFGEDIPLSTVFEASTIAVLAEHIDAIALASQPTAPGVAADEDQEEGEL","length":4314,"molWeight":472985,"crc64":"3C18E71FBE1FDDD1","md5":"85878C05757B1119FFC6B935D5C19C18"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":6,"COFACTOR":2,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":5,"Region":4,"Compositional bias":3,"Active site":3,"Modified residue":3},"uniParcId":"UPI001AF92C31"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TLV7","uniProtkbId":"A0A8A4TLV7_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":18,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD50092.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"value":"Sulfate adenylyltransferase subunit 1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"value":"2.7.7.4"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"value":"ATP-sulfurylase large subunit"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"value":"Sulfate adenylate transferase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"value":"SAT"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD50092.1"}],"value":"cysN"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD50092.1"}],"value":"J3U87_31290"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00002357"}],"value":"APS kinase catalyzes the synthesis of activated sulfate"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"value":"With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24152"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:58243"},{"database":"ChEBI","id":"CHEBI:58339"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.1.25","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001823"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18133"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16189"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58243"}],"ecNumber":"2.7.7.4","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049370"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"value":"Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"value":"Heterodimer composed of CysD, the smaller subunit, and CysN"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005438"}],"value":"In the C-terminal section; belongs to the APS kinase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007237"}],"value":"In the N-terminal section; belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":237,"modifier":"EXACT"}},"description":"Tr-type G","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51722"}]},{"type":"Region","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"G1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"G2","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"G3","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"G4","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"G5","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Binding site","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022695"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00062"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD50092.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD50092.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD50092.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237379722.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TLV7","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_31290","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AERWAYT","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00140","properties":[{"key":"RectionId","value":"UER00204"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0004020","properties":[{"key":"GoTerm","value":"F:adenylylsulfate kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004781","properties":[{"key":"GoTerm","value":"F:sulfate adenylyltransferase (ATP) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0070814","properties":[{"key":"GoTerm","value":"P:hydrogen sulfide biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000103","properties":[{"key":"GoTerm","value":"P:sulfate assimilation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd02027","properties":[{"key":"EntryName","value":"APSK"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04166","properties":[{"key":"EntryName","value":"CysN_ATPS"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03695","properties":[{"key":"EntryName","value":"CysN_NodQ_II"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04095","properties":[{"key":"EntryName","value":"CysN_NoDQ_III"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000119","properties":[{"key":"EntryName","value":"Sulfate adenylyltransferase subunit 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"2"}]},{"database":"HAMAP","id":"MF_00062","properties":[{"key":"EntryName","value":"Sulf_adenylyltr_sub1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002891","properties":[{"key":"EntryName","value":"APS"}]},{"database":"InterPro","id":"IPR059117","properties":[{"key":"EntryName","value":"APS_kinase_dom"}]},{"database":"InterPro","id":"IPR041757","properties":[{"key":"EntryName","value":"CysN_GTP-bd"}]},{"database":"InterPro","id":"IPR044138","properties":[{"key":"EntryName","value":"CysN_II"}]},{"database":"InterPro","id":"IPR044139","properties":[{"key":"EntryName","value":"CysN_NoDQ_III"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR054696","properties":[{"key":"EntryName","value":"GTP-eEF1A_C"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR011779","properties":[{"key":"EntryName","value":"SO4_adenylTrfase_lsu"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR050100","properties":[{"key":"EntryName","value":"TRAFAC_GTPase_members"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR009001","properties":[{"key":"EntryName","value":"Transl_elong_EF1A/Init_IF2_C"}]},{"database":"NCBIfam","id":"TIGR00455","properties":[{"key":"EntryName","value":"apsK"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF003478","properties":[{"key":"EntryName","value":"cysN"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR02034","properties":[{"key":"EntryName","value":"CysN"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF003013","properties":[{"key":"EntryName","value":"PRK03846.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF004035","properties":[{"key":"EntryName","value":"PRK05506.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR23115","properties":[{"key":"EntryName","value":"TRANSLATION FACTOR"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01583","properties":[{"key":"EntryName","value":"APS_kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22594","properties":[{"key":"EntryName","value":"GTP-eEF1A_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"SUPFAM","id":"SSF50465","properties":[{"key":"EntryName","value":"EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSEPKTHSDELTRFLAEEGEKELVRISTAGSVDDGKSTLIGRLLHDTQNIYEDQLHALADESARGSASRQVDWALLTDGLKAEREQGITIDVAYRYFSSHRRKFILADTPGHEQYTRNMATGASTADLAIVLVDARKGLRRQSKRHAFIAALLGIPHMVVAVNKMDLVGYDEAVFERIRDEFSTFATRLGIPDLRFLPISALVGDNVVATSDRMPWYRGEPLLQIMESVFVAGDRNLVDLRLPVQYVNRPHQDFRGYCGQMVSGILRKGSKIEVLPSGKQAHVASITTYDGELDEAAPPMSITVTLDRELDIGRGDMLIHPHNRPTVNRHLEAMMVWMDEMPLDPETRYLVKQTTRTTTALISEVRYRVDVDNLTRQEAAPLQVNEIGRVVLLAQEDLYFDAYHKNRGTGSLILIHPLTHRTVAAGMIIDRQPSDQLPASMDVTRAPISRRPMRQGRVTRDEVARRLDQRPRTLWLTGLVSSGKSEIAYALERKLFERGKLATVLDGGKMRRGLSRELGFGSAGVAENLRRAAETARLFNEAGLIAICSFVSPVAKLREQVSEIVGPDQFSVVHVDATPAHCEARDETGLYRQAREGLVQNLAGWNSPYEAPDEPDVYLDMTQLSIDACVEKILEFLERTQAD","length":643,"molWeight":71930,"crc64":"403363762FE1EE0D","md5":"5887F1FD353B85A4ADB802C20C47390C"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":2,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":3},"countByFeatureType":{"Domain":1,"Region":5,"Binding site":3},"uniParcId":"UPI001AF0EEED"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TMG7","uniProtkbId":"A0A8A4TMG7_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":13,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51179.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Multifunctional fusion protein"}},"includes":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Acireductone dioxygenase"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Acireductone dioxygenase (Fe(2+)-requiring)"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Acireductone dioxygenase (Ni(2+)-requiring)"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"ARD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"ARD'"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"DHK-MTPene dioxygenase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Fe-ARD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Ni-ARD"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"1.13.11.53"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"1.13.11.54"}]}]},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"value":"Enolase-phosphatase E1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"value":"3.1.3.77"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"value":"2,3-diketo-5-methylthio-1-phosphopentane phosphatase"}}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51179.1"}],"value":"mtnC"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"mtnD"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51179.1"}],"value":"J3U87_01810"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"value":"Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:14161"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:17245"},{"database":"ChEBI","id":"CHEBI:49016"},{"database":"ChEBI","id":"CHEBI:49252"}],"ecNumber":"1.13.11.53","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24504"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:16723"},{"database":"ChEBI","id":"CHEBI:49252"}],"ecNumber":"1.13.11.54","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21700"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:49252"},{"database":"ChEBI","id":"CHEBI:58828"}],"ecNumber":"3.1.3.77","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Fe(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29033"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Binds 1 Fe(2+) cation per monomer."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"value":"Binds 1 Mg(2+) ion per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Ni(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49786"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Binds 1 nickel ion per monomer."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"value":"Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"value":"Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"value":"Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"value":"Belongs to the acireductone dioxygenase (ARD) family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Binding site","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29033"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"ligand":{"name":"Fe(2+)","id":"ChEBI:CHEBI:29033"}},{"type":"Binding site","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49786"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"ligand":{"name":"Ni(2+)","id":"ChEBI:CHEBI:49786"}},{"type":"Binding site","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29033"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"ligand":{"name":"Fe(2+)","id":"ChEBI:CHEBI:29033"}},{"type":"Binding site","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49786"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"ligand":{"name":"Ni(2+)","id":"ChEBI:CHEBI:49786"}},{"type":"Binding site","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29033"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"ligand":{"name":"Fe(2+)","id":"ChEBI:CHEBI:29033"}},{"type":"Binding site","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49786"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"ligand":{"name":"Ni(2+)","id":"ChEBI:CHEBI:49786"}},{"type":"Binding site","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29033"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"ligand":{"name":"Fe(2+)","id":"ChEBI:CHEBI:29033"}},{"type":"Binding site","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49786"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"ligand":{"name":"Ni(2+)","id":"ChEBI:CHEBI:49786"}},{"type":"Site","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"May play a role in metal incorporation in vivo","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}]},{"type":"Site","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"May play a role in transmitting local conformational changes","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}]},{"type":"Site","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Important to generate the dianion","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022605"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"id":"KW-0223","category":"Molecular function","name":"Dioxygenase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023167"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01681"}],"id":"KW-0486","category":"Biological process","name":"Methionine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"id":"KW-0533","category":"Ligand","name":"Nickel"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01682"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51179.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51179.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD51179.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237381311.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TMG7","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_01810","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00904","properties":[{"key":"RectionId","value":"UER00876"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0043715","properties":[{"key":"GoTerm","value":"F:2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0043716","properties":[{"key":"GoTerm","value":"F:2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0010308","properties":[{"key":"GoTerm","value":"F:acireductone dioxygenase (Ni2+-requiring) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0010309","properties":[{"key":"GoTerm","value":"F:acireductone dioxygenase [iron(II)-requiring] activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0043874","properties":[{"key":"GoTerm","value":"F:acireductone synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016151","properties":[{"key":"GoTerm","value":"F:nickel cation binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0033353","properties":[{"key":"GoTerm","value":"P:L-methionine cycle"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd02232","properties":[{"key":"EntryName","value":"cupin_ARD"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01629","properties":[{"key":"EntryName","value":"HAD_EP"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.720.60","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1000","properties":[{"key":"EntryName","value":"HAD superfamily/HAD-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.10","properties":[{"key":"EntryName","value":"Jelly Rolls"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01681","properties":[{"key":"EntryName","value":"Salvage_MtnC"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01682","properties":[{"key":"EntryName","value":"Salvage_MtnD"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR004313","properties":[{"key":"EntryName","value":"ARD"}]},{"database":"InterPro","id":"IPR023956","properties":[{"key":"EntryName","value":"ARD_bac"}]},{"database":"InterPro","id":"IPR023943","properties":[{"key":"EntryName","value":"Enolase-ppase_E1"}]},{"database":"InterPro","id":"IPR036412","properties":[{"key":"EntryName","value":"HAD-like_sf"}]},{"database":"InterPro","id":"IPR006439","properties":[{"key":"EntryName","value":"HAD-SF_hydro_IA"}]},{"database":"InterPro","id":"IPR023214","properties":[{"key":"EntryName","value":"HAD_sf"}]},{"database":"InterPro","id":"IPR014710","properties":[{"key":"EntryName","value":"RmlC-like_jellyroll"}]},{"database":"InterPro","id":"IPR011051","properties":[{"key":"EntryName","value":"RmlC_Cupin_sf"}]},{"database":"NCBIfam","id":"TIGR01691","properties":[{"key":"EntryName","value":"enolase-ppase"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR20371","properties":[{"key":"EntryName","value":"ENOLASE-PHOSPHATASE E1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR20371:SF1","properties":[{"key":"EntryName","value":"ENOLASE-PHOSPHATASE E1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03079","properties":[{"key":"EntryName","value":"ARD"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00702","properties":[{"key":"EntryName","value":"Hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00413","properties":[{"key":"EntryName","value":"HADHALOGNASE"}]},{"database":"SFLD","id":"SFLDG01133","properties":[{"key":"EntryName","value":"C1.5.4:_Enolase-phosphatase_Li"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01129","properties":[{"key":"EntryName","value":"C1.5:_HAD__Beta-PGM__Phosphata"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDF00044","properties":[{"key":"EntryName","value":"enolase-phosphatase"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00003","properties":[{"key":"EntryName","value":"Haloacid_Dehalogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56784","properties":[{"key":"EntryName","value":"HAD-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51182","properties":[{"key":"EntryName","value":"RmlC-like cupins"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNSPTERHTTSLTIFAEDNPHQILEHTTDADRIADLLGQRGIRFERWHAGAPLAEAADQEDILAAYRADVDRIMEENGFKSVDVVRMKPDHPQKAEFRAKFLDEHTHSEDEVRFFVEGSGLFYLHLEGKVHVVHCTKGDFIGVPDGTRHWFDMGNQPEFTAIRFFTNPEGWVANWTKDPIASHFPRFVADEPVQAVVTDIEGTTSSISFVKDILFPYARKHLEGFVTERGNEPEVKAILDDARREAGTPDADGAAIAATMIRWIDEDRKVTPLKALQGLIWEKGYRDGDYQAHLYEDAHRHLERWSKSGLDLYIFSSGSIKAQKLMFEHTTYGDLRPWFKGYFDTTTGPKKEASSYRAIAKSIGLAPERILFLSDIVEELDAATAAGMQTRWLIRDGAQEAHPFHRSVPDFDEITLD","length":417,"molWeight":47343,"crc64":"B6DFE8C7B9A71167","md5":"556A29DFED081734B631703637D01D04"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":3,"COFACTOR":3,"PATHWAY":3,"SUBUNIT":1,"SIMILARITY":2},"countByFeatureType":{"Binding site":8,"Site":3},"uniParcId":"UPI001AF0C84A"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TP54","uniProtkbId":"A0A8A4TP54_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":18,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51330.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"Nitrate reductase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"1.9.6.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"napA"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51330.1"}],"value":"J3U87_02580"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"Catalytic subunit of the nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00055000"}],"value":"Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 Fe(II)-[cytochrome] + nitrate + 2 H(+) = 2 Fe(III)-[cytochrome] + nitrite + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:12909"},{"database":"Rhea","id":"RHEA-COMP:11777"},{"database":"Rhea","id":"RHEA-COMP:11778"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16301"},{"database":"ChEBI","id":"CHEBI:17632"},{"database":"ChEBI","id":"CHEBI:29033"},{"database":"ChEBI","id":"CHEBI:29034"}],"ecNumber":"1.9.6.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052176"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mo-bis(molybdopterin guanine dinucleotide)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:60539"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"[4Fe-4S] cluster","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49883"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"Binds 1 [4Fe-4S] cluster."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"Component of the nitrate reductase NapAB complex composed of NapA and NapB"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"Membrane-associated"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00648"}],"value":"Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008747"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":769,"modifier":"EXACT"}},"description":"Nitrate reductase","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00648"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5049600135"},{"type":"Domain","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":97,"modifier":"EXACT"}},"description":"4Fe-4S Mo/W bis-MGD-type","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51669"}]},{"type":"Binding site","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01004"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01004"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01004"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01004"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":170,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":174,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":257,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":359,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":363,"modifier":"EXACT"},"end":{"value":363,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":468,"modifier":"EXACT"},"end":{"value":468,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":493,"modifier":"EXACT"},"end":{"value":494,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":543,"modifier":"EXACT"},"end":{"value":543,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":659,"modifier":"EXACT"},"end":{"value":668,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":735,"modifier":"EXACT"},"end":{"value":735,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":743,"modifier":"EXACT"},"end":{"value":743,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}},{"type":"Binding site","location":{"start":{"value":760,"modifier":"EXACT"},"end":{"value":760,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60539"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"ligand":{"name":"Mo-bis(molybdopterin guanine dinucleotide)","id":"ChEBI:CHEBI:60539"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022485"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0004","category":"Ligand","name":"4Fe-4S"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023014"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022505"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0500","category":"Ligand","name":"Molybdenum"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023063"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0534","category":"Biological process","name":"Nitrate assimilation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022764"}],"id":"KW-0574","category":"Cellular component","name":"Periplasm"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01630"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51330.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51330.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD51330.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237381461.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TP54","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_02580","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAHFQKI","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009325","properties":[{"key":"GoTerm","value":"C:nitrate reductase complex"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0051539","properties":[{"key":"GoTerm","value":"F:4 iron, 4 sulfur cluster binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0030151","properties":[{"key":"GoTerm","value":"F:molybdenum ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0043546","properties":[{"key":"GoTerm","value":"F:molybdopterin cofactor binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0050140","properties":[{"key":"GoTerm","value":"F:nitrate reductase (cytochrome) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0045333","properties":[{"key":"GoTerm","value":"P:cellular respiration"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0006777","properties":[{"key":"GoTerm","value":"P:Mo-molybdopterin cofactor biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0042128","properties":[{"key":"GoTerm","value":"P:nitrate assimilation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd02791","properties":[{"key":"EntryName","value":"MopB_CT_Nitrate-R-NapA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd02754","properties":[{"key":"EntryName","value":"MopB_Nitrate-R-NapA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.40.20:FF:000005","properties":[{"key":"EntryName","value":"Periplasmic nitrate reductase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.40.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.200.210","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.740","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.228.10","properties":[{"key":"EntryName","value":"Dimethylsulfoxide Reductase, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01630","properties":[{"key":"EntryName","value":"Nitrate_reduct_NapA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR009010","properties":[{"key":"EntryName","value":"Asp_de-COase-like_dom_sf"}]},{"database":"InterPro","id":"IPR041957","properties":[{"key":"EntryName","value":"CT_Nitrate-R-NapA-like"}]},{"database":"InterPro","id":"IPR006657","properties":[{"key":"EntryName","value":"MoPterin_dinucl-bd_dom"}]},{"database":"InterPro","id":"IPR006656","properties":[{"key":"EntryName","value":"Mopterin_OxRdtase"}]},{"database":"InterPro","id":"IPR006963","properties":[{"key":"EntryName","value":"Mopterin_OxRdtase_4Fe-4S_dom"}]},{"database":"InterPro","id":"IPR010051","properties":[{"key":"EntryName","value":"Periplasm_NO3_reductase_lsu"}]},{"database":"InterPro","id":"IPR006311","properties":[{"key":"EntryName","value":"TAT_signal"}]},{"database":"PANTHER","id":"PTHR43105:SF11","properties":[{"key":"EntryName","value":"PERIPLASMIC NITRATE REDUCTASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43105","properties":[{"key":"EntryName","value":"RESPIRATORY NITRATE REDUCTASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04879","properties":[{"key":"EntryName","value":"Molybdop_Fe4S4"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00384","properties":[{"key":"EntryName","value":"Molybdopterin"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01568","properties":[{"key":"EntryName","value":"Molydop_binding"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00926","properties":[{"key":"EntryName","value":"Molybdop_Fe4S4"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50692","properties":[{"key":"EntryName","value":"ADC-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53706","properties":[{"key":"EntryName","value":"Formate dehydrogenase/DMSO reductase, domains 1-3"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51669","properties":[{"key":"EntryName","value":"4FE4S_MOW_BIS_MGD"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51318","properties":[{"key":"EntryName","value":"TAT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVSRRLFIKQVAVGSAVAAAKLLVPGVMFAPALASAEAAELAWHKTPCRFCGVGCGLLVGVAGGRAVAVKGDPASPVNKGLCCVKGYHSVQALYGKDRLTSAQMRKNGKLVDVPLTEALDAIAERMKSTIAEHGKDSVAMYGSGQWTIPDGYVASKLFKGCIGTNNVEANARLCMASAVTGFLTTFGLDEPMGCYEDIDHADVFVLWGNNMAEMHPVLFSRLLERRQRNPKTLIIDLGTRTTRTSYAADKAILFEPQTDLAMANCIAYEIVKQGRVNRDFVKKHVAFKKGKTDIGYGLEEKFAFKDQATPANWDDYRAFLEDYTPEKTSAVSGVSVADLRYLASLYADPNRKVVSFWCMGMNQHTRGTWINNLVYNIHLLVGKISQPGNGPFSLTGQPSACGTVREVGTLTHKLPHGVVMNEKHRKKAAEIWNVPVENISPKPSNHTVAMFRALDRGDIRFMWIQVTNPMVTMPKLRRYREGAKKEGRFIVVSDVYPTPTTDIADVILPSAMWIEREGMYGNSERRTQHFNQLVDPPGEAMSDTLQLIEVARRLGYADKFPQNEETHIREIWEEYTRFHAGPKHGMAPYELLKKRSGVLWPFHEGKETKWRYHAKTDPAASGDSFDFYGKPDHRAWIWLRPYEAAPEIPDREYPFWLCTGRVLEHWHTGSMTRRVPVLHRAMPAAYAELHPDDAARLGVRNGDKVRIVSRRGQLELVVAIDQRGRPVRGHVFVPFFDESLLINEVTLDAYCPISKQPDYKKCAVKLERV","length":769,"molWeight":86137,"crc64":"1E79CF6D6A1B1B01","md5":"A79C8F95D12778CB47A2AB35A1219369"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"PTM":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Binding site":18},"uniParcId":"UPI001AFA2E9C"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TPT7","uniProtkbId":"A0A8A4TPT7_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":19,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51986.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"CTP synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"6.3.4.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"Cytidine 5'-triphosphate synthase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"Cytidine triphosphate synthetase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"CTP synthetase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"CTPS"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"UTP--ammonia ligase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"pyrG"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51986.1"}],"value":"J3U87_05890"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-glutamine + H2O = L-glutamate + NH4(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15889"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:29985"},{"database":"ChEBI","id":"CHEBI:58359"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:26426"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29985"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:37563"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:46398"},{"database":"ChEBI","id":"CHEBI:58359"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"6.3.4.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047781"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"UTP + NH4(+) + ATP = CTP + ADP + phosphate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16597"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:37563"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:46398"},{"database":"ChEBI","id":"CHEBI:456216"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005171"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007533"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"Belongs to the CTP synthase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":266,"modifier":"EXACT"}},"description":"CTP synthase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF06418"}]},{"type":"Domain","location":{"start":{"value":303,"modifier":"EXACT"},"end":{"value":524,"modifier":"EXACT"}},"description":"Glutamine amidotransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00117"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":266,"modifier":"EXACT"}},"description":"Amidoligase domain","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}]},{"type":"Active site","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00605"}]},{"type":"Active site","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"Nucleophile; for glutamine hydrolysis","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}]},{"type":"Active site","location":{"start":{"value":506,"modifier":"EXACT"},"end":{"value":506,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00605"}]},{"type":"Active site","location":{"start":{"value":508,"modifier":"EXACT"},"end":{"value":508,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00605"}]},{"type":"Binding site","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37563"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"CTP","id":"ChEBI:CHEBI:37563","note":"allosteric inhibitor"}},{"type":"Binding site","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:46398"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"UTP","id":"ChEBI:CHEBI:46398"}},{"type":"Binding site","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37563"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"CTP","id":"ChEBI:CHEBI:37563","note":"allosteric inhibitor"}},{"type":"Binding site","location":{"start":{"value":187,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37563"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"CTP","id":"ChEBI:CHEBI:37563","note":"allosteric inhibitor"}},{"type":"Binding site","location":{"start":{"value":187,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:46398"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"UTP","id":"ChEBI:CHEBI:46398"}},{"type":"Binding site","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37563"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"CTP","id":"ChEBI:CHEBI:37563","note":"allosteric inhibitor"}},{"type":"Binding site","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:46398"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"UTP","id":"ChEBI:CHEBI:46398"}},{"type":"Binding site","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":241,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":353,"modifier":"EXACT"},"end":{"value":353,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58359"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"L-glutamine","id":"ChEBI:CHEBI:58359"}},{"type":"Binding site","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":384,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58359"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"L-glutamine","id":"ChEBI:CHEBI:58359"}},{"type":"Binding site","location":{"start":{"value":404,"modifier":"EXACT"},"end":{"value":404,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58359"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"L-glutamine","id":"ChEBI:CHEBI:58359"}},{"type":"Binding site","location":{"start":{"value":461,"modifier":"EXACT"},"end":{"value":461,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58359"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"ligand":{"name":"L-glutamine","id":"ChEBI:CHEBI:58359"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022962"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"id":"KW-0315","category":"Domain","name":"Glutamine amidotransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022598"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022975"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01227"}],"id":"KW-0665","category":"Biological process","name":"Pyrimidine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51986.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD51986.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD51986.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237382097.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TPT7","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_05890","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAACNVR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00159","properties":[{"key":"RectionId","value":"UER00277"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0097268","properties":[{"key":"GoTerm","value":"C:cytoophidium"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0003883","properties":[{"key":"GoTerm","value":"F:CTP synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0042802","properties":[{"key":"GoTerm","value":"F:identical protein binding"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0044210","properties":[{"key":"GoTerm","value":"P:'de novo' CTP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006541","properties":[{"key":"GoTerm","value":"P:L-glutamine metabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019856","properties":[{"key":"GoTerm","value":"P:pyrimidine nucleobase biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd03113","properties":[{"key":"EntryName","value":"CTPS_N"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01746","properties":[{"key":"EntryName","value":"GATase1_CTP_Synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000009","properties":[{"key":"EntryName","value":"CTP synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.880:FF:000002","properties":[{"key":"EntryName","value":"CTP synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.880","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01227","properties":[{"key":"EntryName","value":"PyrG"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR029062","properties":[{"key":"EntryName","value":"Class_I_gatase-like"}]},{"database":"InterPro","id":"IPR004468","properties":[{"key":"EntryName","value":"CTP_synthase"}]},{"database":"InterPro","id":"IPR017456","properties":[{"key":"EntryName","value":"CTP_synthase_N"}]},{"database":"InterPro","id":"IPR017926","properties":[{"key":"EntryName","value":"GATASE"}]},{"database":"InterPro","id":"IPR033828","properties":[{"key":"EntryName","value":"GATase1_CTP_Synthase"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"NCBIfam","id":"NF003792","properties":[{"key":"EntryName","value":"PRK05380.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00337","properties":[{"key":"EntryName","value":"PyrG"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11550","properties":[{"key":"EntryName","value":"CTP SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11550:SF0","properties":[{"key":"EntryName","value":"CTP SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06418","properties":[{"key":"EntryName","value":"CTP_synth_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00117","properties":[{"key":"EntryName","value":"GATase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52317","properties":[{"key":"EntryName","value":"Class I glutamine amidotransferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51273","properties":[{"key":"EntryName","value":"GATASE_TYPE_1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTKYIFVTGGVLSSLGKGLAAASIGALLESRGFRVTIQKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTNMTATQQHNWTTGRIYQKVINKERRGDYLGNTVQVIPHITDEIKNSIKKLGDPETDIVIVEIGGTVGDIESLPFLEAIRQLRLEVGIGEAINLHLTYVPYLRAAQELKSKPTQHSVKELRSIGIQPDIVLCRCEYDLPDDLCRKVALFCNVPTDAVKVAIDVNNIYKVPLKLAQQNLDSLICRLLNLPEGKPDLTTWEELSGIIDSPRPEIHIGVVGKYVDYPDSYKSIIEAFNHSGFFHRVHVKLSWLESANYEKGLNLEELERLDGILIPGGFGNRGIEGMIRAIQLARETGKPFFGICLGMQLASVEFARNVAQLDDANSSEFEDDPTNKIIYKLRELVDIDEMGGTMRLGAYECELEPGSLAARAYGRESISERHRHRYEFNIEFKEKLEEAGLLFTGISPNGTFMEIIELPNHPWFLGCQFHPEFKSKPFAPHPLFRDFVGASIKHRETRLKAQAEPEKEAVS","length":547,"molWeight":61384,"crc64":"0A42AE1E49F0C613","md5":"DE99A5D045314AFA65C1068713F90233"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"ACTIVITY REGULATION":1,"PATHWAY":1,"SUBUNIT":1,"MISCELLANEOUS":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":2,"Region":1,"Active site":4,"Binding site":16},"uniParcId":"UPI001AFA41AC"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TTW7","uniProtkbId":"A0A8A4TTW7_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":23,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53409.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00072270"}],"value":"Bifunctional aspartokinase/homoserine dehydrogenase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013213"}],"value":"1.1.1.3"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013059"}],"value":"2.7.2.4"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53409.1"}],"value":"thrA"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53409.1"}],"value":"J3U87_13220"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00044938"}],"value":"Bifunctional aspartate kinase and homoserine dehydrogenase that catalyzes the first and the third steps toward the synthesis of lysine, methionine and threonine from aspartate"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-aspartate + ATP = 4-phospho-L-aspartate + ADP","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23776"},{"database":"ChEBI","id":"CHEBI:29991"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:57535"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.2.4","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048561"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:23777"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048561"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-homoserine + NAD(+) = L-aspartate 4-semialdehyde + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15757"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57476"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:537519"}],"ecNumber":"1.1.1.3","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049031"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:15759"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049031"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-homoserine + NADP(+) = L-aspartate 4-semialdehyde + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15761"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57476"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:537519"}],"ecNumber":"1.1.1.3","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048841"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:15763"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048841"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"a metal cation","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001920"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:25213"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004766"}],"value":"Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004986"}],"value":"Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005062"}],"value":"Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005139"}],"value":"Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005056"}],"value":"Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011881"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007952"}],"value":"In the C-terminal section; belongs to the homoserine dehydrogenase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010046"}],"value":"In the N-terminal section; belongs to the aspartokinase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":401,"modifier":"EXACT"},"end":{"value":479,"modifier":"EXACT"}},"description":"ACT","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51671"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022605"}],"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022777"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53409.1"}],"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023154"}],"id":"KW-0457","category":"Biological process","name":"Lysine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023167"}],"id":"KW-0486","category":"Biological process","name":"Methionine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53409.1"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023053"}],"id":"KW-0915","category":"Ligand","name":"Sodium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022697"}],"id":"KW-0791","category":"Biological process","name":"Threonine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53409.1"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53409.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53409.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD53409.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237383512.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TTW7","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_13220","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AACCEIW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00034","properties":[{"key":"RectionId","value":"UER00015"}]},{"database":"UniPathway","id":"UPA00050","properties":[{"key":"RectionId","value":"UER00063"}]},{"database":"UniPathway","id":"UPA00051","properties":[{"key":"RectionId","value":"UER00462"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0004072","properties":[{"key":"GoTerm","value":"F:aspartate kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004412","properties":[{"key":"GoTerm","value":"F:homoserine dehydrogenase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0050661","properties":[{"key":"GoTerm","value":"F:NADP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0009090","properties":[{"key":"GoTerm","value":"P:L-homoserine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0009088","properties":[{"key":"GoTerm","value":"P:L-threonine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"CDD","id":"cd04243","properties":[{"key":"EntryName","value":"AAK_AK-HSDH-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04892","properties":[{"key":"EntryName","value":"ACT_AK-like_2"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04921","properties":[{"key":"EntryName","value":"ACT_AKi-HSDH-ThrA-like_1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.2130.10:FF:000001","properties":[{"key":"EntryName","value":"Bifunctional aspartokinase/homoserine dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.360.10:FF:000006","properties":[{"key":"EntryName","value":"Bifunctional aspartokinase/homoserine dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1160.10","properties":[{"key":"EntryName","value":"Acetylglutamate kinase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.360.10","properties":[{"key":"EntryName","value":"Dihydrodipicolinate Reductase, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.2130.10","properties":[{"key":"EntryName","value":"VC0802-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036393","properties":[{"key":"EntryName","value":"AceGlu_kinase-like_sf"}]},{"database":"InterPro","id":"IPR045865","properties":[{"key":"EntryName","value":"ACT-like_dom_sf"}]},{"database":"InterPro","id":"IPR054352","properties":[{"key":"EntryName","value":"ACT_Aspartokinase"}]},{"database":"InterPro","id":"IPR002912","properties":[{"key":"EntryName","value":"ACT_dom"}]},{"database":"InterPro","id":"IPR049638","properties":[{"key":"EntryName","value":"AK-HD"}]},{"database":"InterPro","id":"IPR001048","properties":[{"key":"EntryName","value":"Asp/Glu/Uridylate_kinase"}]},{"database":"InterPro","id":"IPR005106","properties":[{"key":"EntryName","value":"Asp/hSer_DH_NAD-bd"}]},{"database":"InterPro","id":"IPR001341","properties":[{"key":"EntryName","value":"Asp_kinase"}]},{"database":"InterPro","id":"IPR018042","properties":[{"key":"EntryName","value":"Aspartate_kinase_CS"}]},{"database":"InterPro","id":"IPR011147","properties":[{"key":"EntryName","value":"Bifunc_Aspkin/hSer_DH"}]},{"database":"InterPro","id":"IPR001342","properties":[{"key":"EntryName","value":"HDH_cat"}]},{"database":"InterPro","id":"IPR019811","properties":[{"key":"EntryName","value":"HDH_CS"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"NCBIfam","id":"TIGR00657","properties":[{"key":"EntryName","value":"asp_kinases"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF006959","properties":[{"key":"EntryName","value":"thrA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43070","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43070:SF5","properties":[{"key":"EntryName","value":"HOMOSERINE DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00696","properties":[{"key":"EntryName","value":"AA_kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22468","properties":[{"key":"EntryName","value":"ACT_9"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF00742","properties":[{"key":"EntryName","value":"Homoserine_dh"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03447","properties":[{"key":"EntryName","value":"NAD_binding_3"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000727","properties":[{"key":"EntryName","value":"ThrA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55021","properties":[{"key":"EntryName","value":"ACT-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF53633","properties":[{"key":"EntryName","value":"Carbamate kinase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55347","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51671","properties":[{"key":"EntryName","value":"ACT"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00324","properties":[{"key":"EntryName","value":"ASPARTOKINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01042","properties":[{"key":"EntryName","value":"HOMOSER_DHGENASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIVMKFGGSSLGTPARVRAVMDIVANARRRFGAAAVVCSAFGGATDQLISMGKAASSGSLYYQETLHAFEKRHLDAVAELFPNGNRSAVEARIRNLVGDLSDVLHGIFLVRELTARTQDFITSFGERLSARIIAEALAAGGLPAEYLDARTLIRTDDRYGSARVQFEDTNRRITEYFEKHDALQIVTGFIAASDQQQTTTLGRGGSDYTASIFGAALGAHEIQIWTDVDGVLTADPRKVGDAFPIPEISYEEAMEMAHFGAKVIYPPTMAPAMTAGIPLRIKNTFQPEAAGTLVGRRVEQDHPLPACGLSSIDHVTLLQLKGCGLFGAAGVANRLFGALARASVNIILISQGSSEHSICVAVAPTDAHRARKAADEEFALELMTKQVDPIAVEENKSVIAVVGDQMRRVPGIAGRVFNALGANQINVVAIAQGSSERNVSFVVRHEDLKPALHVLHQAIFFPLRRTLHLFLVGIGLVGRELVQQIAAKEAGAGEEQPRIRVVGLANSKKMIFDPNGLGLDDLEGTLARDGLPMDGEALLARAQAMQVSDKILVDLTASDVVPRWYEPALRSGISVVTANKKANTAEQAYFDKLKSLVSCHGASFLYETNVGAGLPVIRPLKDLIAGGDAVAHIEGMLSGTLSYLFNAFDGSRPFSELVLAARDSGFTEPDPREDLNGMDVARKLLILARECGHRLELDDLEVESLIPEELRGLDSAEDFMARLSEADAMFEGRRTEAAENGQVLRYIARFADGKGVVSLQAVGSDHPFYHTRGSDNLVSYTTRRYATTPMVIKGPGAGAVVTAGGVLADILQVGRGH","length":817,"molWeight":87729,"crc64":"019B2316691E34AD","md5":"EDE23B382C167A1409D36702FC0DB95B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"COFACTOR":1,"PATHWAY":5,"SUBUNIT":1,"SIMILARITY":2},"countByFeatureType":{"Domain":1},"uniParcId":"UPI001AF7417B"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TU24","uniProtkbId":"A0A8A4TU24_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":17,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52618.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"Riboflavin biosynthesis protein RibBA"}},"includes":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"3,4-dihydroxy-2-butanone 4-phosphate synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"DHBP synthase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"4.1.99.12"}]}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"GTP cyclohydrolase-2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"3.5.4.25"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"GTP cyclohydrolase II"}}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52618.1"}],"value":"ribB"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"ribBA"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52618.1"}],"value":"J3U87_09100"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00002284"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00043932"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18457"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:58121"},{"database":"ChEBI","id":"CHEBI:58830"}],"ecNumber":"4.1.99.12","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000141"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 2 phosphate + 3 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23704"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58614"}],"ecNumber":"3.5.4.25","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049295"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"Binds 2 divalent metal cations per subunit. Magnesium or manganese."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001936"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"Binds 1 zinc ion per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004904"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004853"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008976"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"In the C-terminal section; belongs to the GTP cyclohydrolase II family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005520"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"value":"In the N-terminal section; belongs to the DHBP synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"GTP cyclohydrolase II","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00925"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":203,"modifier":"EXACT"}},"description":"DHBP synthase","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}]},{"type":"Region","location":{"start":{"value":204,"modifier":"EXACT"},"end":{"value":407,"modifier":"EXACT"}},"description":"GTP cyclohydrolase II","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}]},{"type":"Active site","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"Proton acceptor; for GTP cyclohydrolase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}]},{"type":"Active site","location":{"start":{"value":331,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"Nucleophile; for GTP cyclohydrolase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}]},{"type":"Binding site","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58121"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"D-ribulose 5-phosphate","id":"ChEBI:CHEBI:58121"}},{"type":"Binding site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58121"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"D-ribulose 5-phosphate","id":"ChEBI:CHEBI:58121"}},{"type":"Binding site","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58121"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"D-ribulose 5-phosphate","id":"ChEBI:CHEBI:58121"}},{"type":"Binding site","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58121"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"D-ribulose 5-phosphate","id":"ChEBI:CHEBI:58121"}},{"type":"Binding site","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":257,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":268,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":270,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":273,"modifier":"EXACT"},"end":{"value":273,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":295,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":317,"modifier":"EXACT"},"end":{"value":317,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":352,"modifier":"EXACT"},"end":{"value":352,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":357,"modifier":"EXACT"},"end":{"value":357,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Site","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":128,"modifier":"EXACT"}},"description":"Essential for DHBP synthase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}]},{"type":"Site","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"Essential for DHBP synthase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023211"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0464","category":"Ligand","name":"Manganese"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022619"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0686","category":"Biological process","name":"Riboflavin biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022833"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01283"}],"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52618.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD52618.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD52618.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237382722.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TU24","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_09100","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ADEHHMK","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00275","properties":[{"key":"RectionId","value":"UER00399"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0008686","properties":[{"key":"GoTerm","value":"F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003935","properties":[{"key":"GoTerm","value":"F:GTP cyclohydrolase II activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0030145","properties":[{"key":"GoTerm","value":"F:manganese ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008270","properties":[{"key":"GoTerm","value":"F:zinc ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009231","properties":[{"key":"GoTerm","value":"P:riboflavin biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00641","properties":[{"key":"EntryName","value":"GTP_cyclohydro2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.10990:FF:000001","properties":[{"key":"EntryName","value":"Riboflavin biosynthesis protein RibBA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.870.10:FF:000001","properties":[{"key":"EntryName","value":"Riboflavin biosynthesis protein RibBA"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.870.10","properties":[{"key":"EntryName","value":"DHBP synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.10990","properties":[{"key":"EntryName","value":"GTP cyclohydrolase II"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00179","properties":[{"key":"EntryName","value":"RibA"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00180","properties":[{"key":"EntryName","value":"RibB"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01283","properties":[{"key":"EntryName","value":"RibBA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR017945","properties":[{"key":"EntryName","value":"DHBP_synth_RibB-like_a/b_dom"}]},{"database":"InterPro","id":"IPR000422","properties":[{"key":"EntryName","value":"DHBP_synthase_RibB"}]},{"database":"InterPro","id":"IPR032677","properties":[{"key":"EntryName","value":"GTP_cyclohydro_II"}]},{"database":"InterPro","id":"IPR000926","properties":[{"key":"EntryName","value":"RibA"}]},{"database":"InterPro","id":"IPR036144","properties":[{"key":"EntryName","value":"RibA-like_sf"}]},{"database":"InterPro","id":"IPR016299","properties":[{"key":"EntryName","value":"Riboflavin_synth_RibBA"}]},{"database":"NCBIfam","id":"NF001591","properties":[{"key":"EntryName","value":"ribA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00505","properties":[{"key":"EntryName","value":"ribA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00506","properties":[{"key":"EntryName","value":"ribB"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21327:SF18","properties":[{"key":"EntryName","value":"3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21327","properties":[{"key":"EntryName","value":"GTP CYCLOHYDROLASE II-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00926","properties":[{"key":"EntryName","value":"DHBP_synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00925","properties":[{"key":"EntryName","value":"GTP_cyclohydro2"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001259","properties":[{"key":"EntryName","value":"RibA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF142695","properties":[{"key":"EntryName","value":"RibA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55821","properties":[{"key":"EntryName","value":"YrdC/RibB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPLASIKEALEDIKAGRFVIVVDDEDRENEGDLTMAGAHVTPEAINFMTKFGRGLICAPLAAELFDRLNIPLMVPSQHNASKYGTNFGVSISAIDGITTGISAHDRALSVKALAEEGAGPEDIRMPGHIFPLRARANGVLTRRGHTEAAVDLARLAGSTSAGVICEILKDDGSMARLPDLEVFAEEHGIKIISIEDLAAYRRQKENIVSRIDAATIPTDYGTFQAIAYQDHNGQEHLAFCYGEKSEEGVLVRLHSACLTGDVFMSRRCDCGPQLKKALKEISTKGQGIVLYLAQEGRGIGLANKIRAYALQEKGLDTVEANLHLGFPDDMRTYDVGAAILRDLGIREVCLMTNNPRKVEELEACGIKVRERVPHQIQPHEDNDGYLRTKASKLGHLLEDGHDWRRDA","length":407,"molWeight":44529,"crc64":"ADC4B1D89DCD14E0","md5":"4A1CB503FF038DA518417D827DFF937C"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":2,"COFACTOR":3,"PATHWAY":2,"SIMILARITY":2},"countByFeatureType":{"Domain":1,"Region":2,"Active site":2,"Binding site":16,"Site":2},"uniParcId":"UPI001AF46303"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TUN8","uniProtkbId":"A0A8A4TUN8_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":18,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53230.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"Bifunctional NAD(P)H-hydrate repair enzyme"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"Nicotinamide nucleotide repair protein"}}],"includes":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"ADP-dependent (S)-NAD(P)H-hydrate dehydratase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"4.2.1.136"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"ADP-dependent NAD(P)HX dehydratase"}}]},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"NAD(P)H-hydrate epimerase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"5.1.99.6"}]}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"value":"nnrD"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"value":"nnrE"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53230.1"}],"value":"J3U87_12305"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00025153"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"value":"Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"value":"Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(6R)-NADHX = (6S)-NADHX","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:32215"},{"database":"ChEBI","id":"CHEBI:64074"},{"database":"ChEBI","id":"CHEBI:64075"}],"ecNumber":"5.1.99.6","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000013"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(6R)-NADPHX = (6S)-NADPHX","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:32227"},{"database":"ChEBI","id":"CHEBI:64076"},{"database":"ChEBI","id":"CHEBI:64077"}],"ecNumber":"5.1.99.6","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000909"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(6S)-NADHX + ADP = AMP + phosphate + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:32223"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:64074"},{"database":"ChEBI","id":"CHEBI:456215"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"4.2.1.136","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048238"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(6S)-NADPHX + ADP = AMP + phosphate + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:32235"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:64076"},{"database":"ChEBI","id":"CHEBI:456215"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"4.2.1.136","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049209"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"K(+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29103"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"Binds 1 potassium ion per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"value":"Belongs to the NnrD/CARKD family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"value":"Belongs to the NnrE/AIBP family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009524"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"In the C-terminal section; belongs to the NnrD/CARKD family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006001"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR017184"}],"value":"In the N-terminal section; belongs to the NnrE/AIBP family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"YjeF N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51385"}]},{"type":"Domain","location":{"start":{"value":222,"modifier":"EXACT"},"end":{"value":480,"modifier":"EXACT"}},"description":"YjeF C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51383"}]},{"type":"Binding site","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:64076"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"ligand":{"name":"(6S)-NADPHX","id":"ChEBI:CHEBI:64076"}},{"type":"Binding site","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29103"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"ligand":{"name":"K(+)","id":"ChEBI:CHEBI:29103"}},{"type":"Binding site","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29103"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"ligand":{"name":"K(+)","id":"ChEBI:CHEBI:29103"}},{"type":"Binding site","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":136,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:64076"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"ligand":{"name":"(6S)-NADPHX","id":"ChEBI:CHEBI:64076"}},{"type":"Binding site","location":{"start":{"value":159,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:64076"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"ligand":{"name":"(6S)-NADPHX","id":"ChEBI:CHEBI:64076"}},{"type":"Binding site","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29103"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"ligand":{"name":"K(+)","id":"ChEBI:CHEBI:29103"}},{"type":"Binding site","location":{"start":{"value":257,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:64076"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"ligand":{"name":"(6S)-NADPHX","id":"ChEBI:CHEBI:64076"}},{"type":"Binding site","location":{"start":{"value":311,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:64076"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"ligand":{"name":"(6S)-NADPHX","id":"ChEBI:CHEBI:64076"}},{"type":"Binding site","location":{"start":{"value":358,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:64076"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"ligand":{"name":"(6S)-NADPHX","id":"ChEBI:CHEBI:64076"}},{"type":"Binding site","location":{"start":{"value":396,"modifier":"EXACT"},"end":{"value":400,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:456215"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"ligand":{"name":"AMP","id":"ChEBI:CHEBI:456215"}},{"type":"Binding site","location":{"start":{"value":425,"modifier":"EXACT"},"end":{"value":425,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:456215"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"ligand":{"name":"AMP","id":"ChEBI:CHEBI:456215"}},{"type":"Binding site","location":{"start":{"value":426,"modifier":"EXACT"},"end":{"value":426,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:64076"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"ligand":{"name":"(6S)-NADPHX","id":"ChEBI:CHEBI:64076"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01965"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022958"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01966"}],"id":"KW-0630","category":"Ligand","name":"Potassium"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53230.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53230.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD53230.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237383330.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TUN8","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_12305","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAAMQWN","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0052855","properties":[{"key":"GoTerm","value":"F:ADP-dependent NAD(P)H-hydrate dehydratase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0052856","properties":[{"key":"GoTerm","value":"F:NAD(P)HX epimerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0110051","properties":[{"key":"GoTerm","value":"P:metabolite repair"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0046496","properties":[{"key":"GoTerm","value":"P:nicotinamide nucleotide metabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd01171","properties":[{"key":"EntryName","value":"YXKO-related"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1190.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.10260","properties":[{"key":"EntryName","value":"YjeF N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01965","properties":[{"key":"EntryName","value":"NADHX_dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01966","properties":[{"key":"EntryName","value":"NADHX_epimerase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR017953","properties":[{"key":"EntryName","value":"Carbohydrate_kinase_pred_CS"}]},{"database":"InterPro","id":"IPR000631","properties":[{"key":"EntryName","value":"CARKD"}]},{"database":"InterPro","id":"IPR030677","properties":[{"key":"EntryName","value":"Nnr"}]},{"database":"InterPro","id":"IPR029056","properties":[{"key":"EntryName","value":"Ribokinase-like"}]},{"database":"InterPro","id":"IPR004443","properties":[{"key":"EntryName","value":"YjeF_N_dom"}]},{"database":"InterPro","id":"IPR036652","properties":[{"key":"EntryName","value":"YjeF_N_dom_sf"}]},{"database":"NCBIfam","id":"TIGR00196","properties":[{"key":"EntryName","value":"yjeF_cterm"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00197","properties":[{"key":"EntryName","value":"yjeF_nterm"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12592:SF0","properties":[{"key":"EntryName","value":"ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12592","properties":[{"key":"EntryName","value":"ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01256","properties":[{"key":"EntryName","value":"Carb_kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03853","properties":[{"key":"EntryName","value":"YjeF_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF017184","properties":[{"key":"EntryName","value":"Nnr"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53613","properties":[{"key":"EntryName","value":"Ribokinase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF64153","properties":[{"key":"EntryName","value":"YjeF N-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01050","properties":[{"key":"EntryName","value":"YJEF_C_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51383","properties":[{"key":"EntryName","value":"YJEF_C_3"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51385","properties":[{"key":"EntryName","value":"YJEF_N"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKAIVSASEMAAADRYTIERLGVPGIELMERAARACVGELMEELRPDSLVTVLAGPGNNGGDGLAIARLLHGEGVTVRVFLLQDGETYRGDAALNHQRLRDLAVDISVIEDPATFGLPLGCDWVVDALFGTGLSRPITGGLANLIERLNVSGLRVLAVDIPSGLSGDHGRVDGPAVRARLTVTFQCLKFAHVVTPACLSCGDVRVHDIGISFSDEARTGSWLVEGRDFQRPTRDVEGHKGRYGYLGVVGGFAGMAGAASLAARAALRFGAGKVRVLTDQPAYFGQPGSVMVDHVDRWKQVGDFDALVIGPGLSRAEAAWSHLRQWDLRDRSIVWDADGLHLLAAERLFPGKDWVMTPHPGEAAVLLGTTAGEVQRDRLAAIRALAKAFPGGWLLLKGFRTLVLSPGGELFVCGTGNVALATAGSGDVLSGMIGAMLAQGCAMEEAVLSACLRHGLAADRWVRHHPDYSMIAEDIIEDLKY","length":480,"molWeight":51081,"crc64":"B5F6A6FEE8E927A4","md5":"F75C7D3ED4E9498E1A904189D3B392A2"},"extraAttributes":{"countByCommentType":{"FUNCTION":3,"CATALYTIC ACTIVITY":4,"COFACTOR":2,"SUBUNIT":1,"SIMILARITY":4,"CAUTION":1},"countByFeatureType":{"Domain":2,"Binding site":12},"uniParcId":"UPI001AF5AA3F"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TVR0","uniProtkbId":"A0A8A4TVR0_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":15,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54046.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"Coenzyme A biosynthesis bifunctional protein CoaBC"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"DNA/pantothenate metabolism flavoprotein"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"Phosphopantothenoylcysteine synthetase/decarboxylase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"PPCS-PPCDC"}]}],"includes":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"Phosphopantothenoylcysteine decarboxylase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"PPC decarboxylase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"PPC-DC"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"4.1.1.36"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"CoaC"}}]},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"Phosphopantothenate--cysteine ligase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"6.3.2.5"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"CoaB"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"Phosphopantothenoylcysteine synthetase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"PPC synthetase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"PPC-S"}]}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54046.1"}],"value":"coaBC"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54046.1"}],"value":"J3U87_16495"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}],"value":"Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4-phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19397"},{"database":"ChEBI","id":"CHEBI:10986"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:35235"},{"database":"ChEBI","id":"CHEBI:37563"},{"database":"ChEBI","id":"CHEBI:59458"},{"database":"ChEBI","id":"CHEBI:60377"}],"ecNumber":"6.3.2.5","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N-[(R)-4-phosphopantothenoyl]-L-cysteine + H(+) = (R)-4'-phosphopantetheine + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16793"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:59458"},{"database":"ChEBI","id":"CHEBI:61723"}],"ecNumber":"4.1.1.36","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"FMN","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:58210"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"Binds 1 FMN per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}],"value":"Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}],"value":"Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}],"value":"In the C-terminal section; belongs to the PPC synthetase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}],"value":"In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"Flavoprotein","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02441"}]},{"type":"Domain","location":{"start":{"value":195,"modifier":"EXACT"},"end":{"value":402,"modifier":"EXACT"}},"description":"DNA/pantothenate metabolism flavoprotein C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04127"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":198,"modifier":"EXACT"}},"description":"Phosphopantothenoylcysteine decarboxylase","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}]},{"type":"Region","location":{"start":{"value":199,"modifier":"EXACT"},"end":{"value":405,"modifier":"EXACT"}},"description":"Phosphopantothenate--cysteine ligase","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}]},{"type":"Active site","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}]},{"type":"Binding site","location":{"start":{"value":287,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37563"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"ligand":{"name":"CTP","id":"ChEBI:CHEBI:37563"}},{"type":"Binding site","location":{"start":{"value":296,"modifier":"EXACT"},"end":{"value":296,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37563"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"ligand":{"name":"CTP","id":"ChEBI:CHEBI:37563"}},{"type":"Binding site","location":{"start":{"value":331,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37563"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"ligand":{"name":"CTP","id":"ChEBI:CHEBI:37563"}},{"type":"Binding site","location":{"start":{"value":345,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37563"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"ligand":{"name":"CTP","id":"ChEBI:CHEBI:37563"}},{"type":"Binding site","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":349,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37563"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"ligand":{"name":"CTP","id":"ChEBI:CHEBI:37563"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022793"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"id":"KW-0210","category":"Molecular function","name":"Decarboxylase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}],"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}],"id":"KW-0288","category":"Ligand","name":"FMN"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364078"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02225"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54046.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54046.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD54046.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237384146.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TVR0","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_16495","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AACHFIT","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00241","properties":[{"key":"RectionId","value":"UER00353"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0071513","properties":[{"key":"GoTerm","value":"C:phosphopantothenoylcysteine decarboxylase complex"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0010181","properties":[{"key":"GoTerm","value":"F:FMN binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004632","properties":[{"key":"GoTerm","value":"F:phosphopantothenate--cysteine ligase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004633","properties":[{"key":"GoTerm","value":"F:phosphopantothenoylcysteine decarboxylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0015937","properties":[{"key":"GoTerm","value":"P:coenzyme A biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0015941","properties":[{"key":"GoTerm","value":"P:pantothenate catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.40.50.10300","properties":[{"key":"EntryName","value":"CoaB-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1950","properties":[{"key":"EntryName","value":"Flavin prenyltransferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_02225","properties":[{"key":"EntryName","value":"CoaBC"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR035929","properties":[{"key":"EntryName","value":"CoaB-like_sf"}]},{"database":"InterPro","id":"IPR005252","properties":[{"key":"EntryName","value":"CoaBC"}]},{"database":"InterPro","id":"IPR007085","properties":[{"key":"EntryName","value":"DNA/pantothenate-metab_flavo_C"}]},{"database":"InterPro","id":"IPR036551","properties":[{"key":"EntryName","value":"Flavin_trans-like"}]},{"database":"InterPro","id":"IPR003382","properties":[{"key":"EntryName","value":"Flavoprotein"}]},{"database":"NCBIfam","id":"TIGR00521","properties":[{"key":"EntryName","value":"coaBC_dfp"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR14359","properties":[{"key":"EntryName","value":"HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR14359:SF6","properties":[{"key":"EntryName","value":"PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04127","properties":[{"key":"EntryName","value":"DFP"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02441","properties":[{"key":"EntryName","value":"Flavoprotein"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF102645","properties":[{"key":"EntryName","value":"CoaB-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52507","properties":[{"key":"EntryName","value":"Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTGHTEEGGHEGLSIALGVTGGIAAYKTVELVRLLTQRGITVYPIMTEWAARFVGPLTFETLTGQPVRIATPSAVETGNIEHISLIRSVDLLLIAPLTANTMAKMAHGQADNFLTTTYLAHRGKTVGCPAMNTGMLEHPATRRNMNQLRQDGVHLVLGDSGDLACGEVGAGRMAEPSVILDHLDWVLSPRLPRFEGKRVLVTAGPTREDLDPVRFLTNRSSGKMGIAIARAFRDAGAQVTLIHGPIQVPLPAMVEGFGVRSAAEMANAVLSRQMEQDVIVMAAAVADYRPRPSVHKLKKESFDGRLHLERTTDILATLGSRKPDHQILVGFAAETGSVLEHAARKIARKNLDFIFANDVSAEGLGFAGDRNRLIALNRGGVQHDLGTRDKERLGHDLVRYLASQL","length":405,"molWeight":43796,"crc64":"54EA727566839DA2","md5":"59C1D55DC36D21AFC25D60258EA579F3"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":2,"COFACTOR":2,"PATHWAY":2,"SIMILARITY":2,"CAUTION":1},"countByFeatureType":{"Domain":2,"Region":2,"Active site":1,"Binding site":5},"uniParcId":"UPI001AF420A3"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TWB0","uniProtkbId":"A0A8A4TWB0_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":19,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53780.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"Carbamoyl phosphate synthase large chain"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"6.3.4.16"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"6.3.5.5"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"Carbamoyl phosphate synthetase ammonia chain"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53780.1"}],"value":"carB"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53780.1"}],"value":"J3U87_15125"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"Large subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18633"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17544"},{"database":"ChEBI","id":"CHEBI:29985"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58228"},{"database":"ChEBI","id":"CHEBI:58359"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"6.3.5.5","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18029"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17544"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58228"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"6.3.4.16","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047359"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"Binds 4 Mg(2+) or Mn(2+) ions per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005077"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"The large subunit is composed of 2 ATP-grasp domains that are involved in binding the 2 ATP molecules needed for carbamoyl phosphate synthesis. The N-terminal ATP-grasp domain (referred to as the carboxyphosphate synthetic component) catalyzes the ATP-dependent phosphorylation of hydrogencarbonate to carboxyphosphate and the subsequent nucleophilic attack by ammonia to form a carbamate intermediate. The C-terminal ATP-grasp domain (referred to as the carbamoyl phosphate synthetic component) then catalyzes the phosphorylation of carbamate with the second ATP to form the end product carbamoyl phosphate. The reactive and unstable enzyme intermediates are sequentially channeled from one active site to the next through the interior of the protein over a distance of at least 96 A"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009799"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"Belongs to the CarB family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":1069,"modifier":"EXACT"}},"description":"Carbamoyl phosphate synthase large chain","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}],"featureId":"PRO_5049436013"},{"type":"Domain","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":328,"modifier":"EXACT"}},"description":"ATP-grasp","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50975"}]},{"type":"Domain","location":{"start":{"value":671,"modifier":"EXACT"},"end":{"value":862,"modifier":"EXACT"}},"description":"ATP-grasp","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50975"}]},{"type":"Domain","location":{"start":{"value":929,"modifier":"EXACT"},"end":{"value":1069,"modifier":"EXACT"}},"description":"MGS-like","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51855"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":402,"modifier":"EXACT"}},"description":"Carboxyphosphate synthetic domain","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}]},{"type":"Region","location":{"start":{"value":929,"modifier":"EXACT"},"end":{"value":1069,"modifier":"EXACT"}},"description":"Allosteric domain","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}]},{"type":"Binding site","location":{"start":{"value":129,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":169,"modifier":"EXACT"},"end":{"value":169,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":176,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":208,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":210,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":215,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":241,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":242,"modifier":"EXACT"},"end":{"value":242,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":243,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":285,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":285,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":285,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035","label":"1"}},{"type":"Binding site","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":299,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"1"}},{"type":"Binding site","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":299,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":299,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":299,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035","label":"1"}},{"type":"Binding site","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":299,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035","label":"2"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035","label":"2"}},{"type":"Binding site","location":{"start":{"value":707,"modifier":"EXACT"},"end":{"value":707,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":746,"modifier":"EXACT"},"end":{"value":746,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":748,"modifier":"EXACT"},"end":{"value":748,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":753,"modifier":"EXACT"},"end":{"value":753,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":778,"modifier":"EXACT"},"end":{"value":778,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":779,"modifier":"EXACT"},"end":{"value":779,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":780,"modifier":"EXACT"},"end":{"value":780,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":781,"modifier":"EXACT"},"end":{"value":781,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":821,"modifier":"EXACT"},"end":{"value":821,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":821,"modifier":"EXACT"},"end":{"value":821,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Binding site","location":{"start":{"value":821,"modifier":"EXACT"},"end":{"value":821,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035","label":"3"}},{"type":"Binding site","location":{"start":{"value":833,"modifier":"EXACT"},"end":{"value":833,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616","label":"2"}},{"type":"Binding site","location":{"start":{"value":833,"modifier":"EXACT"},"end":{"value":833,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"3"}},{"type":"Binding site","location":{"start":{"value":833,"modifier":"EXACT"},"end":{"value":833,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"4"}},{"type":"Binding site","location":{"start":{"value":833,"modifier":"EXACT"},"end":{"value":833,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035","label":"3"}},{"type":"Binding site","location":{"start":{"value":833,"modifier":"EXACT"},"end":{"value":833,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035","label":"4"}},{"type":"Binding site","location":{"start":{"value":835,"modifier":"EXACT"},"end":{"value":835,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"4"}},{"type":"Binding site","location":{"start":{"value":835,"modifier":"EXACT"},"end":{"value":835,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035","label":"4"}},{"type":"Lipidation","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"N-palmitoyl cysteine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Lipidation","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"S-diacylglycerol cysteine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022605"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022571"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"id":"KW-0055","category":"Biological process","name":"Arginine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022598"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}],"id":"KW-0449","category":"PTM","name":"Lipoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023211"}],"id":"KW-0464","category":"Ligand","name":"Manganese"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}],"id":"KW-0564","category":"PTM","name":"Palmitate"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022975"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"id":"KW-0665","category":"Biological process","name":"Pyrimidine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022737"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01210"}],"id":"KW-0677","category":"Domain","name":"Repeat"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00303"}],"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53780.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD53780.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD53780.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237383880.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TWB0","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_15125","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00068","properties":[{"key":"RectionId","value":"UER00171"}]},{"database":"UniPathway","id":"UPA00070","properties":[{"key":"RectionId","value":"UER00115"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004087","properties":[{"key":"GoTerm","value":"F:carbamoyl-phosphate synthase (ammonia) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004088","properties":[{"key":"GoTerm","value":"F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0044205","properties":[{"key":"GoTerm","value":"P:'de novo' UMP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006526","properties":[{"key":"GoTerm","value":"P:L-arginine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006541","properties":[{"key":"GoTerm","value":"P:L-glutamine metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd01424","properties":[{"key":"EntryName","value":"MGS_CPS_II"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.470.20:FF:000007","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase large chain"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.470.20:FF:000026","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase large chain"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.20:FF:000001","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase large chain"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.40.50.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.30.1490.20","properties":[{"key":"EntryName","value":"ATP-grasp fold, A domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.470.20","properties":[{"key":"EntryName","value":"ATP-grasp fold, B domain"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"1.10.1030.10","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1380","properties":[{"key":"EntryName","value":"Methylglyoxal synthase-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01210_B","properties":[{"key":"EntryName","value":"CPSase_L_chain_B"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR011761","properties":[{"key":"EntryName","value":"ATP-grasp"}]},{"database":"InterPro","id":"IPR013815","properties":[{"key":"EntryName","value":"ATP_grasp_subdomain_1"}]},{"database":"InterPro","id":"IPR036897","properties":[{"key":"EntryName","value":"CarbamoylP_synth_lsu_oligo_sf"}]},{"database":"InterPro","id":"IPR005479","properties":[{"key":"EntryName","value":"CPAse_ATP-bd"}]},{"database":"InterPro","id":"IPR005483","properties":[{"key":"EntryName","value":"CPSase_dom"}]},{"database":"InterPro","id":"IPR006275","properties":[{"key":"EntryName","value":"CPSase_lsu"}]},{"database":"InterPro","id":"IPR005480","properties":[{"key":"EntryName","value":"CPSase_lsu_oligo"}]},{"database":"InterPro","id":"IPR058047","properties":[{"key":"EntryName","value":"CPSase_preATP-grasp"}]},{"database":"InterPro","id":"IPR011607","properties":[{"key":"EntryName","value":"MGS-like_dom"}]},{"database":"InterPro","id":"IPR036914","properties":[{"key":"EntryName","value":"MGS-like_dom_sf"}]},{"database":"InterPro","id":"IPR033937","properties":[{"key":"EntryName","value":"MGS_CPS_CarB"}]},{"database":"InterPro","id":"IPR016185","properties":[{"key":"EntryName","value":"PreATP-grasp_dom_sf"}]},{"database":"NCBIfam","id":"NF003671","properties":[{"key":"EntryName","value":"carB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01369","properties":[{"key":"EntryName","value":"CPSaseII_lrg"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009455","properties":[{"key":"EntryName","value":"PRK12815.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11405:SF53","properties":[{"key":"EntryName","value":"CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11405","properties":[{"key":"EntryName","value":"CARBAMOYLTRANSFERASE FAMILY MEMBER"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25596","properties":[{"key":"EntryName","value":"CPSase_L_D1"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF02786","properties":[{"key":"EntryName","value":"CPSase_L_D2"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF02787","properties":[{"key":"EntryName","value":"CPSase_L_D3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02142","properties":[{"key":"EntryName","value":"MGS"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00098","properties":[{"key":"EntryName","value":"CPSASE"}]},{"database":"SMART","id":"SM01096","properties":[{"key":"EntryName","value":"CPSase_L_D3"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00851","properties":[{"key":"EntryName","value":"MGS"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48108","properties":[{"key":"EntryName","value":"Carbamoyl phosphate synthetase, large subunit connection domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56059","properties":[{"key":"EntryName","value":"Glutathione synthetase ATP-binding domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52335","properties":[{"key":"EntryName","value":"Methylglyoxal synthase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52440","properties":[{"key":"EntryName","value":"PreATP-grasp domain"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS50975","properties":[{"key":"EntryName","value":"ATP_GRASP"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS00866","properties":[{"key":"EntryName","value":"CPSASE_1"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS00867","properties":[{"key":"EntryName","value":"CPSASE_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51855","properties":[{"key":"EntryName","value":"MGS"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51257","properties":[{"key":"EntryName","value":"PROKAR_LIPOPROTEIN"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPKREDIKSVLLIGSGPITIGQACEFDYSGVQAIRALKEEGIRVILVNSNPATVMTDPDLADVTYIEPLTPSFLTTIIEQERPDALLPTMGGQTALNLAMLLDERGILERFGVELIGASVEAITLGEDRLRFRDLMTENGLETCRGGFAHSMDEARTIQADIGFPVIIRPSFTLGGSGGGVAHTREEFEAIADSGLTASPNSQILIEESIIGWKEFELEVIRDKADNGIIICSIENLDAMGVHTGDSITVAPAQTLSDREYQNLRDISLKVLRLVGVETGGANVQFAVDPRSDRVVVVEMNPRVSRSSALASKATGYPIAKVAAKLALGYTLDELKNQITRVTPASFEPSLDYVAVKFPRWAFEKFPRANATLTTQMKSVGEVMALGRSFKEAFLKAVQSLEQGRHHLGIPRSEAPDDLEERLRVPSWLRMRSIFHALRAGWTIDRIYALTHIDPWFLAHFRDIVELERDLARYEPEQIPEEQLRLAKYWGLSDAGIAVLSGTDESVITARTSRFPYGFKAVDTCAGEFPAETPYLYGASGEESDVTPLPGPKIAIIGSGPNRIGQGIEFDYCCVQAVNGFKQAGYQTIMINCNPETVSTDFDVADRLYFEPLILERVACILAFEKPDAVAVAFGGQTSLNLAEPLAKLGYRIIGTSSDIIHKVEDRETFAKIIEKTGLRQAEHRTAANIEEARRLAAEIGFPLMVRPSFVLGGRAMSIVFDSDDLEQSLKMAMEATPNQAIFIDRFLDDAIELDVDALSDGKDIVIVGIMQHIEEAGIHSGDSSCVLPPVLLTPTQVAEVKRATRALAKELDVVGLLNVQYAYWRDALYVIEANPRCSRTVPFAAKAIGIPIPEMAARVMAGATIGELAIPLEPEPYMWHVKSPVFPFNKLQGEDPVLGPEMKSTGEVMGSAHSFGNAFAKAYRATGYKLPREGTAFLSVTDNDKPVILLVARDLYQLGFELIATRGTAEFLRQQGLPVETIRKRHQGSPNIVDRIHERKVALIINTPVGKKSHEDDRYIRFEALRLNVPCITTTSAAQAVVSAIRAIKSERLMVESLQSLAKTRKTS","length":1069,"molWeight":117351,"crc64":"634E2FCBD36457BD","md5":"5766BC0AFD22B754DBD631BA30CCA486"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"PATHWAY":2,"SUBUNIT":1,"DOMAIN":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":3,"Region":2,"Binding site":38,"Lipidation":2},"uniParcId":"UPI001AF0E12F"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TYT3","uniProtkbId":"A0A8A4TYT3_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":17,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54421.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00074141"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"value":"Catalase-peroxidase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"value":"CP"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00067012"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"value":"1.11.1.21"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"value":"Peroxidase/catalase"}}],"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54421.1"}],"value":"katG"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54421.1"}],"value":"J3U87_26275"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"value":"Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 H2O2 = O2 + 2 H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20309"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16240"}],"ecNumber":"1.11.1.21","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049145"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003451"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"AH2 + H2O2 = A + 2 H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:30275"},{"database":"ChEBI","id":"CHEBI:13193"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:16240"},{"database":"ChEBI","id":"CHEBI:17499"}],"ecNumber":"1.11.1.21","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051651"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003451"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"NADH + O2 + H(+) = H2O2 + NAD(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11264"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16240"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"1.6.3.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10012"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"NADPH + O2 + H(+) = H2O2 + NADP(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11260"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16240"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"ecNumber":"1.6.3.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10012"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"heme b","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:60344"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"value":"Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"value":"Homodimer or homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"value":"Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00060838"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003451"}],"value":"Belongs to the peroxidase family. Peroxidase/catalase subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003451"}]},{"type":"Chain","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":735,"modifier":"EXACT"}},"description":"Catalase-peroxidase","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003451"}],"featureId":"PRO_5051573152"},{"type":"Domain","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":428,"modifier":"EXACT"}},"description":"Plant heme peroxidase family profile","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50873"}]},{"type":"Active site","location":{"start":{"value":103,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10012"}]},{"type":"Binding site","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60344"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"ligand":{"name":"heme b","id":"ChEBI:CHEBI:60344"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}},{"type":"Site","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}]},{"type":"Cross-link","location":{"start":{"value":224,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with Trp-102)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022617"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"id":"KW-0349","category":"Ligand","name":"Heme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023324"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"id":"KW-0376","category":"Biological process","name":"Hydrogen peroxide"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022559"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"}],"id":"KW-0575","category":"Molecular function","name":"Peroxidase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01961"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003451"}],"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54421.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD54421.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD54421.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A8A4TYT3","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_26275","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AFDWVQT","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004096","properties":[{"key":"GoTerm","value":"F:catalase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0020037","properties":[{"key":"GoTerm","value":"F:heme binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016174","properties":[{"key":"GoTerm","value":"F:NAD(P)H oxidase H2O2-forming activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0070301","properties":[{"key":"GoTerm","value":"P:cellular response to hydrogen peroxide"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0042744","properties":[{"key":"GoTerm","value":"P:hydrogen peroxide catabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd00649","properties":[{"key":"EntryName","value":"catalase_peroxidase_1"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd08200","properties":[{"key":"EntryName","value":"catalase_peroxidase_2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.420.10:FF:000002","properties":[{"key":"EntryName","value":"Catalase-peroxidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.420.10:FF:000004","properties":[{"key":"EntryName","value":"Catalase-peroxidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.520.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"1.10.420.10","properties":[{"key":"EntryName","value":"Peroxidase, domain 2"},{"key":"MatchStatus","value":"2"}]},{"database":"HAMAP","id":"MF_01961","properties":[{"key":"EntryName","value":"Catal_peroxid"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR000763","properties":[{"key":"EntryName","value":"Catalase_peroxidase"}]},{"database":"InterPro","id":"IPR002016","properties":[{"key":"EntryName","value":"Haem_peroxidase"}]},{"database":"InterPro","id":"IPR010255","properties":[{"key":"EntryName","value":"Haem_peroxidase_sf"}]},{"database":"InterPro","id":"IPR019794","properties":[{"key":"EntryName","value":"Peroxidases_AS"}]},{"database":"InterPro","id":"IPR019793","properties":[{"key":"EntryName","value":"Peroxidases_heam-ligand_BS"}]},{"database":"NCBIfam","id":"TIGR00198","properties":[{"key":"EntryName","value":"cat_per_HPI"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF011635","properties":[{"key":"EntryName","value":"PRK15061.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30555:SF0","properties":[{"key":"EntryName","value":"CATALASE-PEROXIDASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30555","properties":[{"key":"EntryName","value":"HYDROPEROXIDASE I, BIFUNCTIONAL CATALASE-PEROXIDASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00141","properties":[{"key":"EntryName","value":"peroxidase"},{"key":"MatchStatus","value":"2"}]},{"database":"PRINTS","id":"PR00460","properties":[{"key":"EntryName","value":"BPEROXIDASE"}]},{"database":"PRINTS","id":"PR00458","properties":[{"key":"EntryName","value":"PEROXIDASE"}]},{"database":"SUPFAM","id":"SSF48113","properties":[{"key":"EntryName","value":"Heme-dependent peroxidases"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS00435","properties":[{"key":"EntryName","value":"PEROXIDASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00436","properties":[{"key":"EntryName","value":"PEROXIDASE_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50873","properties":[{"key":"EntryName","value":"PEROXIDASE_4"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSLIASLLVVSLTPLTAGGPTAQKGEAKSNQFWWPEQLNLAPLRAHDPASNPYGEKFDYAAAFKTLDLKAVKKDINAILTESQAWWPADYGNYGPFFIRMAWHSAGTYRTADGRGGAAGGQMRFDPLNSWPDNANLDKARRLLWPVKQKYGRNLSWADLIVLTGNVAMENMGFKTLGFAGGREDDWEPDLVYWGPEAKFEGDKRHSGNRKLDRPLAATQMGLIYVNPQGPNGNPDPLAAAHDIRVTFGRMGMNDEETVALIAGGHTFGKAHGAHKAKHVGAEPAAAPTEEQGFGWKNTAGKGKAEDTFTSGLEGAWTSAPARWTHMFLTNLYAFEWKQTRSPAGAIQWVPTDESIKNVPDAHVQGKFHAPIMLTTDLSLRFDPSYQKISKRFLDNPQEFEKAFARAWFKLTHRDMGPRARYLGSEVPREEMAWQDPVPKQNHKLINAKEVEKLKARILDSGLTVPELVRTAWASASSFRGSDMRGGANGARVRLAPQKDWEANSPQELAKVLDKLEKIQTSFQKSLSRGKKVSLADVIVLGGAAAIEKAAKDGGYKNVKVPFTPGRMDASQKQTNVASFNELEQKADGFRNFFAAGNYRSPAEMLVDKANLLNLTVPEMTVLVGGMRALNANTDQSRHGVFTDRPGTLSNDFFVNLLDMSTKWKKSASSEGVYEGYDRATNKLKWTATPVDLIFGSNSELRAVTEVYAFEDSREKFARDFVAAWTKVMNSDRFDL","length":735,"molWeight":81345,"crc64":"515FA7DB19917421","md5":"F33398541CE7A24D46A35BD7FCAF8231"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":4,"COFACTOR":1,"SUBUNIT":1,"PTM":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":1,"Binding site":1,"Site":1,"Cross-link":1},"uniParcId":"UPI001AF62023"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TC67","uniProtkbId":"A0A8A4TC67_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":20,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47699.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"value":"Tyrosine--tRNA ligase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"value":"6.1.1.1"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"value":"Tyrosyl-tRNA synthetase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"value":"TyrRS"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"value":"tyrS"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47699.1"}],"value":"J3U87_19080"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"value":"Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10220"},{"database":"Rhea","id":"RHEA-COMP:9706"},{"database":"Rhea","id":"RHEA-COMP:9707"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:58315"},{"database":"ChEBI","id":"CHEBI:78442"},{"database":"ChEBI","id":"CHEBI:78536"},{"database":"ChEBI","id":"CHEBI:456215"}],"ecNumber":"6.1.1.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048248"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00060965"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"value":"Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":358,"modifier":"EXACT"},"end":{"value":420,"modifier":"EXACT"}},"description":"RNA-binding S4","evidences":[{"evidenceCode":"ECO:0000259","source":"SMART","id":"SM00363"}]},{"type":"Motif","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"'HIGH' region","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}]},{"type":"Motif","location":{"start":{"value":231,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"'KMSKS' region","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}]},{"type":"Binding site","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58315"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"ligand":{"name":"L-tyrosine","id":"ChEBI:CHEBI:58315"}},{"type":"Binding site","location":{"start":{"value":171,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58315"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"ligand":{"name":"L-tyrosine","id":"ChEBI:CHEBI:58315"}},{"type":"Binding site","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58315"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"ligand":{"name":"L-tyrosine","id":"ChEBI:CHEBI:58315"}},{"type":"Binding site","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023146"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"id":"KW-0030","category":"Molecular function","name":"Aminoacyl-tRNA synthetase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022598"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022917"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02006"}],"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022884"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00182"}],"id":"KW-0694","category":"Molecular function","name":"RNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47699.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47699.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47699.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377366.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TC67","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_19080","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAECFKM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0042803","properties":[{"key":"GoTerm","value":"F:protein homodimerization activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0003723","properties":[{"key":"GoTerm","value":"F:RNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004831","properties":[{"key":"GoTerm","value":"F:tyrosine-tRNA ligase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006437","properties":[{"key":"GoTerm","value":"P:tyrosyl-tRNA aminoacylation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00165","properties":[{"key":"EntryName","value":"S4"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00805","properties":[{"key":"EntryName","value":"TyrRS_core"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.240.10:FF:000001","properties":[{"key":"EntryName","value":"Tyrosine--tRNA ligase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.620:FF:000008","properties":[{"key":"EntryName","value":"Tyrosine--tRNA ligase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.620","properties":[{"key":"EntryName","value":"HUPs"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.10.290.10","properties":[{"key":"EntryName","value":"RNA-binding S4 domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.240.10","properties":[{"key":"EntryName","value":"Tyrosyl-Transfer RNA Synthetase"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_02006","properties":[{"key":"EntryName","value":"Tyr_tRNA_synth_type1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002305","properties":[{"key":"EntryName","value":"aa-tRNA-synth_Ic"}]},{"database":"InterPro","id":"IPR014729","properties":[{"key":"EntryName","value":"Rossmann-like_a/b/a_fold"}]},{"database":"InterPro","id":"IPR002942","properties":[{"key":"EntryName","value":"S4_RNA-bd"}]},{"database":"InterPro","id":"IPR036986","properties":[{"key":"EntryName","value":"S4_RNA-bd_sf"}]},{"database":"InterPro","id":"IPR054608","properties":[{"key":"EntryName","value":"SYY-like_C"}]},{"database":"InterPro","id":"IPR002307","properties":[{"key":"EntryName","value":"Tyr-tRNA-ligase"}]},{"database":"InterPro","id":"IPR024088","properties":[{"key":"EntryName","value":"Tyr-tRNA-ligase_bac-type"}]},{"database":"InterPro","id":"IPR024107","properties":[{"key":"EntryName","value":"Tyr-tRNA-ligase_bac_1"}]},{"database":"NCBIfam","id":"TIGR00234","properties":[{"key":"EntryName","value":"tyrS"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11766:SF0","properties":[{"key":"EntryName","value":"TYROSINE--TRNA LIGASE, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11766","properties":[{"key":"EntryName","value":"TYROSYL-TRNA SYNTHETASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22421","properties":[{"key":"EntryName","value":"SYY_C-terminal"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00579","properties":[{"key":"EntryName","value":"tRNA-synt_1b"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01040","properties":[{"key":"EntryName","value":"TRNASYNTHTYR"}]},{"database":"SMART","id":"SM00363","properties":[{"key":"EntryName","value":"S4"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55174","properties":[{"key":"EntryName","value":"Alpha-L RNA-binding motif"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52374","properties":[{"key":"EntryName","value":"Nucleotidylyl transferase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50889","properties":[{"key":"EntryName","value":"S4"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKASVLEEFEWRGLVYDKTEGLGQWLAEEKVSAYIGFDPTADSLHVGSLLPIMGLVRLQQYGHRPIALVGGGTGMIGDPSGKSKERNLLDEEQLAKNVEGIRNQLAKFLDFETKENPAKLVNNYDWLGKMNFIEFLRDVGKNFTVNYMMAKESVKRRINSEEGISYTEFTYMMLQAYDFYKLSQSEDCYLQMGGSDQWGNITAGIDYIRRQGGKKAHGLVFPLVTNSSGAKFGKSEEGNIWLDPEKTSPYRFYQFWLNSSDSDVLGYLKNFTMLDQDQIAELGHRLETDPASRAAQKRLAEEITRTVHGQEELDKVQRASKVMFGGSVEGLTAADISAIIREMPATDLARENFEGEGFWVPELLVSCGVTKSKGEARRLVTSGAVYVNNQRVADANHKIVLDQAIDGRFLVLRKGKKNYHLVKILS","length":426,"molWeight":47874,"crc64":"08C464EA22F909B6","md5":"D9734222CBBF88C014549C1FD68E1770"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Motif":2,"Binding site":4},"uniParcId":"UPI001AF0DF84"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TCR0","uniProtkbId":"A0A8A4TCR0_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":16,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47726.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"Pantothenate synthetase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"PS"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"6.3.2.1"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"Pantoate--beta-alanine ligase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"Pantoate-activating enzyme"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"panC"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47726.1"}],"value":"J3U87_19220"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(R)-pantoate + beta-alanine + ATP = (R)-pantothenate + AMP + diphosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10912"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15980"},{"database":"ChEBI","id":"CHEBI:29032"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:57966"},{"database":"ChEBI","id":"CHEBI:456215"}],"ecNumber":"6.3.2.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048258"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004990"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"The reaction proceeds by a bi uni uni bi ping pong mechanism"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009256"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"value":"Belongs to the pantothenate synthetase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Active site","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}]},{"type":"Binding site","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15980"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"ligand":{"name":"(R)-pantoate","id":"ChEBI:CHEBI:15980"}},{"type":"Binding site","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57966"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"ligand":{"name":"beta-alanine","id":"ChEBI:CHEBI:57966"}},{"type":"Binding site","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15980"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"ligand":{"name":"(R)-pantoate","id":"ChEBI:CHEBI:15980"}},{"type":"Binding site","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":178,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":186,"modifier":"EXACT"},"end":{"value":189,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022598"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022655"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00158"}],"id":"KW-0566","category":"Biological process","name":"Pantothenate biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47726.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47726.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47726.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377392.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TCR0","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_19220","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AACWLGK","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00028","properties":[{"key":"RectionId","value":"UER00005"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004592","properties":[{"key":"GoTerm","value":"F:pantoate-beta-alanine ligase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0015940","properties":[{"key":"GoTerm","value":"P:pantothenate biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00560","properties":[{"key":"EntryName","value":"PanC"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.620","properties":[{"key":"EntryName","value":"HUPs"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1300.10","properties":[{"key":"EntryName","value":"Pantoate-beta-alanine ligase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00158","properties":[{"key":"EntryName","value":"PanC"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003721","properties":[{"key":"EntryName","value":"Pantoate_ligase"}]},{"database":"InterPro","id":"IPR042176","properties":[{"key":"EntryName","value":"Pantoate_ligase_C"}]},{"database":"InterPro","id":"IPR014729","properties":[{"key":"EntryName","value":"Rossmann-like_a/b/a_fold"}]},{"database":"NCBIfam","id":"TIGR00018","properties":[{"key":"EntryName","value":"panC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21299","properties":[{"key":"EntryName","value":"CYTIDYLATE KINASE/PANTOATE-BETA-ALANINE LIGASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21299:SF1","properties":[{"key":"EntryName","value":"PANTOATE--BETA-ALANINE LIGASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02569","properties":[{"key":"EntryName","value":"Pantoate_ligase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52374","properties":[{"key":"EntryName","value":"Nucleotidylyl transferase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAIRLHSHTAELRRMLREQRDQGETIGFVATMGGLHAGHQSLIEASRGDGNFTVVSVFVNPAQFGPNEDFDSYPRDHDSDFELAVEAGGDLIWYPEVEDLYPEEAQTMVQPGPLGQRLCGLSRPQFFPGICTVVLKLFNLIQPHQAYFGAKDFQQLAIIQRMAADFYLDVDVVGCPIVREDDGLAMSSRNLKLEPEDRDLALTLYRTITRAQTAFAEGERDAAKLREALVAEWPEGLELDYLDFRDPEFLEDCAHLTEDTRIFLGAWLNGVRLIDNARLTP","length":281,"molWeight":31630,"crc64":"99CB25AFCD2669AB","md5":"61C9109D1989436B8C5D806FB45053B2"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Active site":1,"Binding site":7},"uniParcId":"UPI001AF02E9A"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TCT1","uniProtkbId":"A0A8A4TCT1_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":18,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47746.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011971"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"Orotate phosphoribosyltransferase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"OPRT"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"OPRTase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011971"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"2.4.2.10"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"pyrE"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47746.1"}],"value":"J3U87_19330"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10380"},{"database":"ChEBI","id":"CHEBI:30839"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:57538"},{"database":"ChEBI","id":"CHEBI:58017"}],"ecNumber":"2.4.2.10","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004889"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"Phosphoribosyltransferase","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00156"}]},{"type":"Binding site","location":{"start":{"value":106,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"in other chain","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":136,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30839"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"orotate","id":"ChEBI:CHEBI:30839"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022676"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022975"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"id":"KW-0665","category":"Biological process","name":"Pyrimidine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01208"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47746.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47746.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47746.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377412.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TCT1","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_19330","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAKPFIF","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00070","properties":[{"key":"RectionId","value":"UER00119"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004588","properties":[{"key":"GoTerm","value":"F:orotate phosphoribosyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0044205","properties":[{"key":"GoTerm","value":"P:'de novo' UMP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019856","properties":[{"key":"GoTerm","value":"P:pyrimidine nucleobase biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd06223","properties":[{"key":"EntryName","value":"PRTases_typeI"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.2020","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01208","properties":[{"key":"EntryName","value":"PyrE"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR023031","properties":[{"key":"EntryName","value":"OPRT"}]},{"database":"InterPro","id":"IPR004467","properties":[{"key":"EntryName","value":"Or_phspho_trans_dom"}]},{"database":"InterPro","id":"IPR029057","properties":[{"key":"EntryName","value":"PRTase-like"}]},{"database":"InterPro","id":"IPR000836","properties":[{"key":"EntryName","value":"PRTase_dom"}]},{"database":"NCBIfam","id":"TIGR00336","properties":[{"key":"EntryName","value":"pyrE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR19278","properties":[{"key":"EntryName","value":"OROTATE PHOSPHORIBOSYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR19278:SF9","properties":[{"key":"EntryName","value":"URIDINE 5'-MONOPHOSPHATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00156","properties":[{"key":"EntryName","value":"Pribosyltran"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53271","properties":[{"key":"EntryName","value":"PRTase-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSQSGSERKATPEQLEIATLLLELGAVSVSPNEPFTWTSGLKSPIYCDNRLIISTVDQRRRVARGFVDLMKQNDWQPDLVAGTATAGIPHGAWVADRLDLPMVYVRSAEKKHGKKNLIEGRVPDGASVVVVEDLISTGGSCIKAAQALEEQGAKVLGVVAIFQYGLPKAAAGFAEANLNYSTLTNMSALLDVAIESGRLSAGDRASLVAWQEDPAAWSVRHGGA","length":224,"molWeight":23856,"crc64":"4E769D160B4BE4B6","md5":"A0E8BFD477F04CC31D82D7E64A7FB8C8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Binding site":5},"uniParcId":"UPI001AF9DB6A"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TCW8","uniProtkbId":"A0A8A4TCW8_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":15,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47523.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Protein-glutamate methylesterase/protein-glutamine glutaminase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"3.1.1.61"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"3.5.1.44"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"cheB"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47523.1"}],"value":"J3U87_18165"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-glutaminyl-[protein] + H2O = L-glutamyl-[protein] + NH4(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16441"},{"database":"Rhea","id":"RHEA-COMP:10207"},{"database":"Rhea","id":"RHEA-COMP:10208"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:29973"},{"database":"ChEBI","id":"CHEBI:30011"}],"ecNumber":"3.5.1.44","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"[protein]-L-glutamate 5-O-methyl ester + H2O = L-glutamyl-[protein] + methanol + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23236"},{"database":"Rhea","id":"RHEA-COMP:10208"},{"database":"Rhea","id":"RHEA-COMP:10311"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17790"},{"database":"ChEBI","id":"CHEBI:29973"},{"database":"ChEBI","id":"CHEBI:82795"}],"ecNumber":"3.1.1.61","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048267"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Belongs to the CheB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"Response regulatory","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50110"}]},{"type":"Domain","location":{"start":{"value":158,"modifier":"EXACT"},"end":{"value":350,"modifier":"EXACT"}},"description":"CheB-type methylesterase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50122"}]},{"type":"Active site","location":{"start":{"value":170,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00050"}]},{"type":"Active site","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00050"}]},{"type":"Active site","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00050"}]},{"type":"Modified residue","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"4-aspartylphosphate","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00169"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022500"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"id":"KW-0145","category":"Biological process","name":"Chemotaxis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00169"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47523.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47523.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47523.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377192.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TCW8","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_18165","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ACGLKEM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000156","properties":[{"key":"GoTerm","value":"F:phosphorelay response regulator activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008984","properties":[{"key":"GoTerm","value":"F:protein-glutamate methylesterase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0050568","properties":[{"key":"GoTerm","value":"F:protein-glutamine glutaminase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006935","properties":[{"key":"GoTerm","value":"P:chemotaxis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd16432","properties":[{"key":"EntryName","value":"CheB_Rec"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd17541","properties":[{"key":"EntryName","value":"REC_CheB-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.2300","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.180","properties":[{"key":"EntryName","value":"Methylesterase CheB, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00099","properties":[{"key":"EntryName","value":"CheB_chemtxs"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008248","properties":[{"key":"EntryName","value":"CheB-like"}]},{"database":"InterPro","id":"IPR035909","properties":[{"key":"EntryName","value":"CheB_C"}]},{"database":"InterPro","id":"IPR011006","properties":[{"key":"EntryName","value":"CheY-like_sf"}]},{"database":"InterPro","id":"IPR000673","properties":[{"key":"EntryName","value":"Sig_transdc_resp-reg_Me-estase"}]},{"database":"InterPro","id":"IPR001789","properties":[{"key":"EntryName","value":"Sig_transdc_resp-reg_receiver"}]},{"database":"NCBIfam","id":"NF001965","properties":[{"key":"EntryName","value":"cheB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009206","properties":[{"key":"EntryName","value":"cheB"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42872","properties":[{"key":"EntryName","value":"PROTEIN-GLUTAMATE METHYLESTERASE/PROTEIN-GLUTAMINE GLUTAMINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42872:SF6","properties":[{"key":"EntryName","value":"PROTEIN-GLUTAMATE METHYLESTERASE_PROTEIN-GLUTAMINE GLUTAMINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01339","properties":[{"key":"EntryName","value":"CheB_methylest"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00072","properties":[{"key":"EntryName","value":"Response_reg"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000876","properties":[{"key":"EntryName","value":"RR_chemtxs_CheB"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00448","properties":[{"key":"EntryName","value":"REC"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52172","properties":[{"key":"EntryName","value":"CheY-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52738","properties":[{"key":"EntryName","value":"Methylesterase CheB, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50122","properties":[{"key":"EntryName","value":"CHEB"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50110","properties":[{"key":"EntryName","value":"RESPONSE_REGULATORY"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MERKKIRVLVVDDSALVRQVISQGLGQDPGIEVVGTASNPYIARDKIIQLRPDVLTLDVEMPRMDGVEFLRKLMPQFPLPVVIVSALTERGKQITLDAMEAGAVEIVTKPSSNVGAGLTIMMQELRTKVKIASTVNVSYWKNRRVPKAAPSSGRRALAESTDKVIGIGASTGGTEAIREILVSLPTDTPGIVIVQHMPPGFTRMFAQRLNQQCNLLVKEATDGDRVIAGQALIAPGDKHVRVVRRGGIYSVKCDAGETVCGHCPSVDVLMESLSREVGANALGVILTGMGRDGANGLLKLRQAGGRTLGQNQESCVVYGMPREAFEIGAVEKQAPLDQMAVEITNKVRSMEKAMKTR","length":357,"molWeight":38474,"crc64":"9E6452C039BD0A83","md5":"F12160F9A287CB56E3A30D87EABE02DA"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Active site":3,"Modified residue":1},"uniParcId":"UPI001AF605C3"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TCX8","uniProtkbId":"A0A8A4TCX8_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":23,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47533.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47533.1"}],"value":"Amino acid adenylation domain-containing protein"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47533.1"}],"value":"J3U87_18215"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00054155"}],"value":"Involved in production of the polyketide antibiotic thailandamide"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"pantetheine 4'-phosphate","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001957"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:47942"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"pyridoxal 5'-phosphate","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001933"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:597326"}}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006432"}],"value":"Belongs to the ATP-dependent AMP-binding enzyme family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00029443"}],"value":"In the C-terminal section; belongs to the NRP synthetase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":617,"modifier":"EXACT"},"end":{"value":694,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":734,"modifier":"EXACT"},"end":{"value":1158,"modifier":"EXACT"}},"description":"Ketosynthase family 3 (KS3)","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52004"}]},{"type":"Domain","location":{"start":{"value":1644,"modifier":"EXACT"},"end":{"value":1722,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":2248,"modifier":"EXACT"},"end":{"value":4127,"modifier":"EXACT"}},"description":"Aminoacyl-transfer RNA synthetases class-II family profile","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50862"}]},{"type":"Domain","location":{"start":{"value":3709,"modifier":"EXACT"},"end":{"value":3783,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":5165,"modifier":"EXACT"},"end":{"value":5240,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":6209,"modifier":"EXACT"},"end":{"value":6284,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":7269,"modifier":"EXACT"},"end":{"value":7344,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Region","location":{"start":{"value":582,"modifier":"EXACT"},"end":{"value":612,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":694,"modifier":"EXACT"},"end":{"value":735,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1728,"modifier":"EXACT"},"end":{"value":1748,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1830,"modifier":"EXACT"},"end":{"value":1861,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":5235,"modifier":"EXACT"},"end":{"value":5258,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":587,"modifier":"EXACT"},"end":{"value":600,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":601,"modifier":"EXACT"},"end":{"value":612,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":696,"modifier":"EXACT"},"end":{"value":706,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":720,"modifier":"EXACT"},"end":{"value":734,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1736,"modifier":"EXACT"},"end":{"value":1748,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":5241,"modifier":"EXACT"},"end":{"value":5255,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":654,"modifier":"EXACT"},"end":{"value":654,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]},{"type":"Modified residue","location":{"start":{"value":1681,"modifier":"EXACT"},"end":{"value":1681,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]},{"type":"Modified residue","location":{"start":{"value":3744,"modifier":"EXACT"},"end":{"value":3744,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]},{"type":"Modified residue","location":{"start":{"value":5200,"modifier":"EXACT"},"end":{"value":5200,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]},{"type":"Modified residue","location":{"start":{"value":6244,"modifier":"EXACT"},"end":{"value":6244,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]},{"type":"Modified residue","location":{"start":{"value":7304,"modifier":"EXACT"},"end":{"value":7304,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022832"}],"id":"KW-0276","category":"Biological process","name":"Fatty acid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022598"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023098"}],"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022450"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}],"id":"KW-0596","category":"PTM","name":"Phosphopantetheine"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022553"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022898"}],"id":"KW-0663","category":"Ligand","name":"Pyridoxal phosphate"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022737"}],"id":"KW-0677","category":"Domain","name":"Repeat"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47533.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47533.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47533.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377202.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"KEGG","id":"scor:J3U87_18215","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0016746","properties":[{"key":"GoTerm","value":"F:acyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016705","properties":[{"key":"GoTerm","value":"F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0031177","properties":[{"key":"GoTerm","value":"F:phosphopantetheine binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0030170","properties":[{"key":"GoTerm","value":"F:pyridoxal phosphate binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0071766","properties":[{"key":"GoTerm","value":"P:Actinobacterium-type cell wall biogenesis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0043041","properties":[{"key":"GoTerm","value":"P:amino acid activation for nonribosomal peptide biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0008610","properties":[{"key":"GoTerm","value":"P:lipid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0072330","properties":[{"key":"GoTerm","value":"P:monocarboxylic acid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0009403","properties":[{"key":"GoTerm","value":"P:toxin biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"CDD","id":"cd05930","properties":[{"key":"EntryName","value":"A_NRPS"},{"key":"MatchStatus","value":"2"}]},{"database":"CDD","id":"cd17643","properties":[{"key":"EntryName","value":"A_NRPS_Cytc1-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd02440","properties":[{"key":"EntryName","value":"AdoMet_MTases"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd05931","properties":[{"key":"EntryName","value":"FAAL"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd19531","properties":[{"key":"EntryName","value":"LCL_NRPS-like"},{"key":"MatchStatus","value":"3"}]},{"database":"CDD","id":"cd00610","properties":[{"key":"EntryName","value":"OAT_like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00833","properties":[{"key":"EntryName","value":"PKS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.12780:FF:000013","properties":[{"key":"EntryName","value":"Long-chain-fatty-acid--AMP ligase FadD32"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.1200.10:FF:000016","properties":[{"key":"EntryName","value":"Non-ribosomal peptide synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.12780:FF:000012","properties":[{"key":"EntryName","value":"Non-ribosomal peptide synthetase"},{"key":"MatchStatus","value":"2"}]},{"database":"FunFam","id":"2.30.38.10:FF:000001","properties":[{"key":"EntryName","value":"Non-ribosomal peptide synthetase PvdI"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.300.30:FF:000015","properties":[{"key":"EntryName","value":"Nonribosomal peptide synthase SidD"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.1200.10:FF:000005","properties":[{"key":"EntryName","value":"Nonribosomal peptide synthetase 1"},{"key":"MatchStatus","value":"2"}]},{"database":"FunFam","id":"3.40.47.10:FF:000019","properties":[{"key":"EntryName","value":"Polyketide synthase type I"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.300.30","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"6"}]},{"database":"Gene3D","id":"3.30.70.3290","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.47.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.980","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"4"}]},{"database":"Gene3D","id":"1.10.1200.10","properties":[{"key":"EntryName","value":"ACP-like"},{"key":"MatchStatus","value":"6"}]},{"database":"Gene3D","id":"3.40.50.1820","properties":[{"key":"EntryName","value":"alpha/beta hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.1150.10","properties":[{"key":"EntryName","value":"Aspartate Aminotransferase, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.559.10","properties":[{"key":"EntryName","value":"Chloramphenicol acetyltransferase-like domain"},{"key":"MatchStatus","value":"4"}]},{"database":"Gene3D","id":"2.30.38.10","properties":[{"key":"EntryName","value":"Luciferase, Domain 3"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.20.20.30","properties":[{"key":"EntryName","value":"Luciferase-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.366.10","properties":[{"key":"EntryName","value":"Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.12780","properties":[{"key":"EntryName","value":"N-terminal domain of ligase-like"},{"key":"MatchStatus","value":"3"}]},{"database":"Gene3D","id":"3.30.559.30","properties":[{"key":"EntryName","value":"Nonribosomal peptide synthetase, condensation domain"},{"key":"MatchStatus","value":"4"}]},{"database":"Gene3D","id":"3.40.640.10","properties":[{"key":"EntryName","value":"Type I PLP-dependent aspartate aminotransferase-like (Major domain)"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.150","properties":[{"key":"EntryName","value":"Vaccinia Virus protein VP39"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006195","properties":[{"key":"EntryName","value":"aa-tRNA-synth_II"}]},{"database":"InterPro","id":"IPR010071","properties":[{"key":"EntryName","value":"AA_adenyl_dom"}]},{"database":"InterPro","id":"IPR029058","properties":[{"key":"EntryName","value":"AB_hydrolase_fold"}]},{"database":"InterPro","id":"IPR001227","properties":[{"key":"EntryName","value":"Ac_transferase_dom_sf"}]},{"database":"InterPro","id":"IPR036736","properties":[{"key":"EntryName","value":"ACP-like_sf"}]},{"database":"InterPro","id":"IPR014043","properties":[{"key":"EntryName","value":"Acyl_transferase_dom"}]},{"database":"InterPro","id":"IPR016035","properties":[{"key":"EntryName","value":"Acyl_Trfase/lysoPLipase"}]},{"database":"InterPro","id":"IPR005814","properties":[{"key":"EntryName","value":"Aminotrans_3"}]},{"database":"InterPro","id":"IPR045851","properties":[{"key":"EntryName","value":"AMP-b_sf"}]},{"database":"InterPro","id":"IPR025110","properties":[{"key":"EntryName","value":"AMP-bd_C"}]},{"database":"InterPro","id":"IPR020845","properties":[{"key":"EntryName","value":"AMP-binding_CS"}]},{"database":"InterPro","id":"IPR000873","properties":[{"key":"EntryName","value":"AMP-dep_synth/lig_dom"}]},{"database":"InterPro","id":"IPR042099","properties":[{"key":"EntryName","value":"ANL_N_sf"}]},{"database":"InterPro","id":"IPR024011","properties":[{"key":"EntryName","value":"Biosynth_lucif-like_mOase_dom"}]},{"database":"InterPro","id":"IPR023213","properties":[{"key":"EntryName","value":"CAT-like_dom_sf"}]},{"database":"InterPro","id":"IPR001242","properties":[{"key":"EntryName","value":"Condensation_dom"}]},{"database":"InterPro","id":"IPR040097","properties":[{"key":"EntryName","value":"FAAL/FAAC"}]},{"database":"InterPro","id":"IPR014031","properties":[{"key":"EntryName","value":"KAS_C"}]},{"database":"InterPro","id":"IPR014030","properties":[{"key":"EntryName","value":"KAS_N"}]},{"database":"InterPro","id":"IPR011251","properties":[{"key":"EntryName","value":"Luciferase-like_dom"}]},{"database":"InterPro","id":"IPR036661","properties":[{"key":"EntryName","value":"Luciferase-like_sf"}]},{"database":"InterPro","id":"IPR016036","properties":[{"key":"EntryName","value":"Malonyl_transacylase_ACP-bd"}]},{"database":"InterPro","id":"IPR013217","properties":[{"key":"EntryName","value":"Methyltransf_12"}]},{"database":"InterPro","id":"IPR020841","properties":[{"key":"EntryName","value":"PKS_Beta-ketoAc_synthase_dom"}]},{"database":"InterPro","id":"IPR020806","properties":[{"key":"EntryName","value":"PKS_PP-bd"}]},{"database":"InterPro","id":"IPR009081","properties":[{"key":"EntryName","value":"PP-bd_ACP"}]},{"database":"InterPro","id":"IPR006162","properties":[{"key":"EntryName","value":"Ppantetheine_attach_site"}]},{"database":"InterPro","id":"IPR015424","properties":[{"key":"EntryName","value":"PyrdxlP-dep_Trfase"}]},{"database":"InterPro","id":"IPR015421","properties":[{"key":"EntryName","value":"PyrdxlP-dep_Trfase_major"}]},{"database":"InterPro","id":"IPR015422","properties":[{"key":"EntryName","value":"PyrdxlP-dep_Trfase_small"}]},{"database":"InterPro","id":"IPR029063","properties":[{"key":"EntryName","value":"SAM-dependent_MTases_sf"}]},{"database":"InterPro","id":"IPR020802","properties":[{"key":"EntryName","value":"TesA-like"}]},{"database":"InterPro","id":"IPR001031","properties":[{"key":"EntryName","value":"Thioesterase"}]},{"database":"InterPro","id":"IPR016039","properties":[{"key":"EntryName","value":"Thiolase-like"}]},{"database":"NCBIfam","id":"TIGR01733","properties":[{"key":"EntryName","value":"AA-adenyl-dom"},{"key":"MatchStatus","value":"4"}]},{"database":"NCBIfam","id":"NF003417","properties":[{"key":"EntryName","value":"dltA"},{"key":"MatchStatus","value":"7"}]},{"database":"NCBIfam","id":"TIGR04020","properties":[{"key":"EntryName","value":"seco_metab_LLM"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45527","properties":[{"key":"EntryName","value":"NONRIBOSOMAL PEPTIDE SYNTHETASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45527:SF14","properties":[{"key":"EntryName","value":"PLIPASTATIN SYNTHASE SUBUNIT B"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00698","properties":[{"key":"EntryName","value":"Acyl_transf_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00202","properties":[{"key":"EntryName","value":"Aminotran_3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00501","properties":[{"key":"EntryName","value":"AMP-binding"},{"key":"MatchStatus","value":"5"}]},{"database":"Pfam","id":"PF13193","properties":[{"key":"EntryName","value":"AMP-binding_C"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF00296","properties":[{"key":"EntryName","value":"Bac_luciferase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00668","properties":[{"key":"EntryName","value":"Condensation"},{"key":"MatchStatus","value":"4"}]},{"database":"Pfam","id":"PF22621","properties":[{"key":"EntryName","value":"CurL-like_PKS_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00109","properties":[{"key":"EntryName","value":"ketoacyl-synt"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02801","properties":[{"key":"EntryName","value":"Ketoacyl-synt_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08242","properties":[{"key":"EntryName","value":"Methyltransf_12"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00550","properties":[{"key":"EntryName","value":"PP-binding"},{"key":"MatchStatus","value":"6"}]},{"database":"Pfam","id":"PF00975","properties":[{"key":"EntryName","value":"Thioesterase"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00827","properties":[{"key":"EntryName","value":"PKS_AT"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00825","properties":[{"key":"EntryName","value":"PKS_KS"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00823","properties":[{"key":"EntryName","value":"PKS_PP"},{"key":"MatchStatus","value":"6"}]},{"database":"SMART","id":"SM01294","properties":[{"key":"EntryName","value":"PKS_PP_betabranch"},{"key":"MatchStatus","value":"2"}]},{"database":"SMART","id":"SM00824","properties":[{"key":"EntryName","value":"PKS_TE"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56801","properties":[{"key":"EntryName","value":"Acetyl-CoA synthetase-like"},{"key":"MatchStatus","value":"5"}]},{"database":"SUPFAM","id":"SSF47336","properties":[{"key":"EntryName","value":"ACP-like"},{"key":"MatchStatus","value":"6"}]},{"database":"SUPFAM","id":"SSF53474","properties":[{"key":"EntryName","value":"alpha/beta-Hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51679","properties":[{"key":"EntryName","value":"Bacterial luciferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52777","properties":[{"key":"EntryName","value":"CoA-dependent acyltransferases"},{"key":"MatchStatus","value":"8"}]},{"database":"SUPFAM","id":"SSF52151","properties":[{"key":"EntryName","value":"FabD/lysophospholipase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53383","properties":[{"key":"EntryName","value":"PLP-dependent transferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55048","properties":[{"key":"EntryName","value":"Probable ACP-binding domain of malonyl-CoA ACP transacylase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53335","properties":[{"key":"EntryName","value":"S-adenosyl-L-methionine-dependent methyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53901","properties":[{"key":"EntryName","value":"Thiolase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50862","properties":[{"key":"EntryName","value":"AA_TRNA_LIGASE_II"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00455","properties":[{"key":"EntryName","value":"AMP_BINDING"},{"key":"MatchStatus","value":"3"}]},{"database":"PROSITE","id":"PS50075","properties":[{"key":"EntryName","value":"CARRIER"},{"key":"MatchStatus","value":"6"}]},{"database":"PROSITE","id":"PS52004","properties":[{"key":"EntryName","value":"KS3_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00012","properties":[{"key":"EntryName","value":"PHOSPHOPANTETHEINE"},{"key":"MatchStatus","value":"4"}]}],"sequence":{"value":"MEDSKPWAHYGGRSLVHLCRHRAERESRDPAFLFLADGTEIRDTWSRDVLDRRARAIAARLQREDLADGTVLLLFPPGIAFMSAFLGCLYAGAVAVPAPPPDPVRLKRTLPRLRNILRDAGSKAILTQERFAGLLKDSGGELGGIEEVAILALEEIGDDLAPTWRAPHIDPDDLAYLQYSSGSTTAPKGIEITHGNIMHNAGLLQRACGYTRETVSCVWMPHFHDFGLVEGLIAPIVFGFPCYIMSPVSFIRRPMNWLSAISRYRVTHSHGPNFAFDLCLERFDPRKLTDVDLSCWKVACNAAEQVRVSTLTKFRETFAPFGFRADAFHPAYGMAETTLVITGVAPGQPVQVRHLETAALSEGSARFVAEPGPGVTSLVGCGGVSESGYRVQIVDAKHRIPLSELVLGEIWVRGDSVARGYWRAERATEAAFRARLADGTGPFLRTGDLGFIAEDQLYICGRLKELVVIRGQNYVPQDIEQVVEDVDPAIRTNGVAAFAFEEEDEEHLGVVCEIDRKSRKSVDVQALIRHIKSALWDHLELEPGALLLVNAGAIPRTSSGKIKRVSVRKRFQADDWSTLHQWRARSGSASPAKATQSRQDGGSEDRSPSRDLAADTELARRILSWLKLRLGDMLDVDPAQIKEDIPFSELGISSVKAVGLVAELEDWSGRAIPSTAVWRYPNAGSLAAFVAGQGSLPPNQSSSTADAGSLPGEGKTPIDAPHRKGREPSSKNGDDPIAIVGLACRFPGGAHDPEAYWDLLQSGRTTARTFPSDRLPPDFFFDQEAGNDQARNTRFGSFLETDIAAFDPAFFGISPREACLMDPQQRLLLEVCWEALEHAGIDPKSLKNTDGSVFLGQSGDDYAHLAHHFKQDADDHAPLGTARSIAAGRISYVFGLLGPSVQIDTSCSSSLVAVAQACTALRAGETGLALCGGVNLILDPRVSLGLNRMGALGSDGVCKVFDAAADGYGRGEGCAVAILKRLSDARADGDRVLACIRGWAVGHDGASNGLTAPNGLAQEALMTRTLAMSGLRSEQVQYVEAHGTGTVLGDPIEVQSLATCYGQGRDQPLLVGSAKASVGHLEAAAGLAGLFKLTLALQKGVIPGQASFKRLNPHLETSRTSIRVVERTMDWPAEPGTRFGAVSSFGINGTLAHLLVADSAANETDPVATDPVATDPVLTNPVDAKHPCELHLTAHSERALQAMAGSWLTTLRRTAPERVVDLCHAATRRTRFRWRLALTADSHADLVERLTAFCNGERRVGTSCGSVRAVSRLGFLFTGQGSQHARMAEDLYREAPRFRAAIDRCAEILAEGDAERFDLRSVLYGPRAEHLHQTGFAQCALFAVGYGLTEIWRERGIVPELLLGHSLGEYLAAHLAGIFSLEDALRLVRARGSLMQALPPGGAMVDVATEEDRVRPLIERESGRVAISALNAPGRLVISGEEASVERILSRLPGVVSHRLRVSHAFHSPLMEPMLADFERVLAGVTFSAAEIPIVSTVTGKLDDGAMSEPSYWLNQITSPVRFRDAIMAADEAGMTASVEIGPRPVLSRWVPACLGGDSRCVPSLQPPKSDRRQLLESLGALFVQGFPVRWESLYANRAYRHLDTPTYPFQRKHYWFDMGALTMNDTDLRPRIVGSSSHTTPNSRDQLAMKALTSMIADLLHMAPGEVDPDALFLELGADSIILFGARKKIEELFGVNVPIQDLFESFNTVRKLAVRLAEVAERMPASAGARVAPEPVPQPLASPHQPPAGYDVPPVPVPKAQPNALPAALGQAPAGYGQLVDRQLQTMERIFRDQLAYAGGGVVPGEPASVVQPQMSSPPVVERVEPAPVRPVPSAEPVADGGAVSESRPSKAGSPVLPPWSVRETRAADLAEEQTRHLADLTEEYTKRTRTSRKMSQDDRSVLADNRVSAGFRMTTKELQYPIFCDRAEGPTFVDLDGNRYLDITMGFGALLFGNHPDFVVQAMQDQLARGLSLGPHTRQAGEAARLVRELTGMERVAFCTTGTEAVMTVLRATRMLTGRRKTVVFQGSYHGHFDGVLTSGNALSGNLEAESGAPGVLPGSVADVISLPYDDPRSLEVIRAQAGDIACVLVEPVQSRRPDLQPGDFLRQLRELTRGEGIPLVFDEMITGFRIHPGGAQAHFGVRADAALYGKVIGGGMPIGVIAGSPAIMDCLDGGMWQFGDESYPAHETTFYAGTYNKHPLSVAAAVAVLSRLKNEGPELQERLNRRTERLVQTLNGFFESHRVPMRVDSFGSLFRFKPMRHMELFFFHLLREGVYVWEGRACFLSTEHTEVHIEQLVHAVQTAVESMRQGGFLPDALPVNLPARVESAAAGGAATDPWRAGRVALKKRVAETEPFVREPERAPAFSLFFFGHYEAGIGAGMYDLLFDCARFADDHDFRAIWVPERHFHAFGGLSPNPSVLAASLAGQTRRLQLCAGSVVLPLHHPVRVAEEWSMVDNLSKGRVGLACASGWNPNDFVLAPKQFEKNREVMYQNLAELTRLWRGNALALSSGEGKTVEVSLHPLPVRSELPLWLTIVSNPETYIRAGELGLRVLTNLMGQTPEELAVNIRLYRESLAANGFPEERAHVTLLLHTYVGDDLDRAREEARQPFFGYLESSLGLFRSILSKQGLPSDLDGLSDEDRRYLLQAAYERYVTQSALIGTPESCAPIVERLVRAGVDEFACLVDFGVDPDKVRSSLIPLDRLRSQCANWAQSHADSSRQMIRVLGATEVQAAPPSVEVETCFPLSLDQQQVWVLTQTHPEASFAYQDLTVLRLDGALDRKALAQAVKRITARHPSLRTALSEDGETQCVFAAHDFAFAPITLGEDDPDRALANWLAQESETPFDLARGPLWRLNLLVLAPERHVLALATHHLLIDGWSVALILEEIGACYSAITTGKGFQPSEAMSYEALLAKAREAVTDEHLAFWRDQLQPLPPQPEIPADFARPKVKSYRGKRLRVDLDVDQPLLRKVGAAAGCTRFMTLLAALDVLVFHLAGQTEFVIGVPSANREDPQSHHTVGYMANLLPVRRRLAADQPFVDHLRQTRSDLGVVFAHQDYPYGDMVKHLVQNRTTDRGPLVNVVFNVETAFSEPVFDGLDVTVLPSPVRFRPFDLSVNIVEFEGRLFAECDFNTDLFAEASIRRFTDYYRNLLQTVLTDPDRPIGRVSLFSEHEALEWRESVVSTLQTFEVAADIASRFERMAAARPEAVALRDGDRSLTYRELDQRANQMARLLRDEGVGPETPVALLLDRSLELVVAMLATLKAGGTYIPLDPRYPVERLEHMVSASGAVHLIGQPQSEENTPWFTGCRVSVADPRLDRHSAGPLERFLAVDQSAYMIFTSGSTGRPKGVVVTHRNVLRLFAATEALYGFDAEDVWTLFHSPAFDFSVWEIWGALLYGGCLVVVPYEVSRTPERFYRLLVDERVTVLNQTPSAFAQLIEVDERQDAVESTSLRWVIFGGEALQPGMLEPWFARHGDERPALVNMYGITETTVHVTEYRLTSADLMHGRSVIGRPIGDLGLLVLDANMEPVPVGVVGELYVAGAGLARGYRGQPGLSADRFLPNPFGPAGSRLYRSGDLARYRPDGSIEYLGRRDHQVKIRGFRIELGEIESVLIEHPRIRQAVVLAHRDNPNDHCRLVAFYRVEDDGDAIEVKSLREHVARRQPDYMVPAQFVAVSEFPLTLNGKLDRGCLVVPDQVIEGKSESPPETETERTLAAIWEQVLGVAPIGRDDRFFEVGGDSILALKMLAQARERDIELDFAQLFEYQRLDRLAEAADAVTEPRQACDADPFADLRVELNLDGVDGVVDVYPMAAMQEGMLFHAELESHHSLYHNVCLYRISVPFEERRFKAALVELFATHDVLRTHFSEPGVATPYQWVREEVEVPLQVFNWRGIDPDRREEVFETWFETEKFRHFDLQRAPLLRFHVLLDHQSGFVLGLTMHHAILDGWSLARMLTDLFLLYTDRRDRLPACPLRYRDFVALERSCVAAGDAASFWRERLGDTEPFALARLLPGNDAIEVEPFGTHEVYIPSEVGRALERLSRDRDVPLKSLALAAHCVALSVLTGSDRVMTGMIANGRPEEEGADRVLGLFVNTLPISCAVGGRTWIDLAENLWRDERDMMPHRRFPISEIFNMQGGELFDTYFNYLHFHVFHELTGVEIEELPGFQRTNFTLCTNFGRDAGDGHLRLVLEYDSGVVDPDRLQAFGDTCLRVMEAMAAEPDLGPDDVSLLSERTWARLQRWNRTEAVLAVHPVTVDFEAVAASFPDAVALVFGNRTLTYSGLNRRANRMAAALRERGVGPETLVGVCLPREPDLVVSLLAVLKAGGAYVPLDPDYPAARLNMMLDDAGVRLLLTHADLVADLEMGDADVLLLDRMHFDTYSESDPKPYPEPDHLAYTIYTSGSTGKPKGVQITHGALQNFLCAMRSEPGLHRDDSLLAVTTVCFDIAALELFLPLTVGARVVLVDRETAVDGRLLCEALERHEVTIMQATPATWRLLLDADWEPMIGFRVLCGGEGLPVDLARRLLALPIRLHHLYGPTETTIWSSTGRLMTDQLGERYAPVGQPIQNTRAYILDGHLNPVSIGAVGDLYLAGSGLARGYAGRPKRTAVAFLPEPFGQEAGSRMYATGDRARFLDDGSIEILGRGDSQVKIRGYRIELGDIEAALESHEQVKQCVVTAPAREDGFRDLHAFLIPADDADRAALNPEQVAQWERVWSETYGDGQAAEDADPTLNLVGWNSSYTGRPLPRRDMLEWVDATVARVADLRPGHILEVGCGLGLLLFRLAPRCQSYWATDLSQAAIDYVGRLARERGLNQVHLETAEACAFETEGKVFDLVLLNSVVQYFPSMSYLVDVLKRAAAAVRPGGVIMIGDVREKRLLEAFQASVLLERTEPGRSARELVEELDRRLMEEEELAIDHQFFKAVVEELPAIAAVELLPKPGTRHNEMNRFRYDVLLHVGNPKKAVSVDATLDWKREVKSLDGLRDLLSQRDAETLHITDVPNERVVSDVFAGRSMRQAGPGDSVADLRERLARCTLGGVDPSTLESSLSEVGRPYAIDWGADRFELLIAPAGALRPRFPMQSSEPKPWSHYGSYPLRAKLHRDLVPRLRAYLAQRLPEYMVPSGFVLLDRLPLTANGKVDRKALVHRAVEKRERQGHWVAPRGVVEEVLASVWQEILGLDRVGCHDSFFELGGHSLLATRLVARVRAAFSVEISVRDVFDRPTLAALARSVEQRRSTSPTVEETPRSSSDAPPRLHFAQRRMWLLEQLDGGSARYLMPAAVLLEGPLDVPALKSAFTLVMARHEPLRMTIATRDGAPVAILGESPDLTLEIEDIASDEVDRLVRNEAVRPFDLSREIPIRVRLARIEPQKHLLLVTLHHIAADAWSLGVLMSELSAAYRDLRQGKSPRLASLSHRYSDFAAWQAHQMQGPAWEQRLDFWRAHLADLPSVLDLPTDRPRPTVQTSHGALFTFELGAEMSRGLAGLAQRESVSLFMLMLSAFQVFLARLSGQDDVAVGTVDANRTRDHWEPLIGFFANTLVLRTRLAEVETFTDVLARVREDLLKVFQYRDMPFEKLVEELQPERNLAFSPLFQVGFTFQNTEHVELDLPGLTVHSVASTEVGAKYDLTLVMETPESGLLGTFEYNADLFEAETIARFVRGFKALLADILAQPRLSWRRLACMPSADRDQVLHAWQVEADEALRDRCFTDLFGDRVREHGSAPALMFEGGFWSYAELDRRSDLLAATFREKGVTSEDRVGVVADRVPLTLAAMLACFKLGAVYVPLDPGYPEPRLRFIVEDAHLTLWVGADSGTMPPLGDSLPRLIWSDDRWDVPVPGLSVRGPSPETGAYLIYTSGSTGRPKGVLVSHRGLPTLAHWQRRTLDVKPGDRVLQFASPSFDASIWETTMAFALGATLCLVGSEHLLPGPPLRQTCARWRITHLTLQPSALKAMPDADLPDLRTVVVAGEACTADVVAHAPKSARLFNAYGPTEDTVCSSAVSCESQEPVTIGTPIDGDRIYLLDEHMQPVPVGVRGELYLSGIGLARGYHRRPGHTARRFVPNPFSGIPGDRMYRTGDLGRYASDGRIVFLGRVDHQVKVRGFRVETAEVEAVLGRCPGVGEHLIVAQSDASGSSRLLAFVTGYEGRQPKEAELRRYLAEELPGYMVPSAIRICAAFPKTPNGKIDRDALLADFHRPAAEASPRRSETLNPLEIGLIAIWESLLDTAPIGTHDNFFAVGGHSLLAAKLMARIHEAFGIELPVKSLFEHPTIEELAVCVRQAATKSAEPTVAIPPRDARTPAPLTHNQERLWFLHRLGSGSAYDIPLALRIRGPLRIGLCREALARLVRRHETLRMRFPVFEDRACQEAMAPESVRLSVVDAFLDADREEEGQIQALLQEAFGEPIDLEGGPSFRAKLWILGDEDHLLCFSWSHLIADLRSVEVFFGEFSETYRRLSRDPDVTMQPSPRVQFADFAQWQRDRLAEQRRVPDLEYWLDRLADLPDGLALPYDRPPTAQVGFRGGIRRFELDGSLRAAVLRVAANRGASPFMVLVGLFAELLARYSGQTDILLGTPVNNRNRPELRDMIGYLVNTLVLRIDCSGDPSFHELLDRVAERVGEALAHAELPFGLLVEELASRRARGHEPLFQVMISYQDVSGADPIPAEHSDLQVTLIEPPVSTAKFHLTLVLTDHGDRIEAGMEYNADLFDAETVDALCDYLVTSTRLRLDAPQRRSSGLSSVHEPCRDSFCLIGPKPTDREDADVAIHQRIDAVARLHVGEPALVYRGESWSYQRLLERADDIALLLMARGFGCEDLVLLGLPRSPELLAAMLAVLKVGAAFVPVDLHAPARRLVGQTESVDLVLTSDDLVSRFEPNAPELLVLPETMAANPTVPQISLSVFPEQLAYVLFTSGSTGVPKGCQITHGNLDHYLDWALSRYVEPESCRRFSWFTPLTFDFTLTGVFCCLLRGGTLHIYDDDTHLGAALRDQLEAGNGVDLIKLTPTHVTLLSQEEVAASETRIAVLGGEALQAEQVRALRRLNPDMRLVNEYGPTEVTVGCLTHEVQSPDEAISIGTPIAGITAYVLDAHGLPVPRGAVGELCLAGAGLSRGYRGMAGRTATSFVPDPYADQPGSRYYRTGDLVRMTRAGELHYLGRQDEQVKINGLRIELGDVAHAMSAHEGVAAAVAGTVTAPSGEAALTVWWVPGDAERAPVSSDIRAFLQVRLPRGMVPRYLVMVPRLPHTRNGKVDRAALPEPDFASGGVGAVRVEPRNVTEHRLRELWQSVLPVAVPGIHDDFFELGGHSMAALMLVSRIERAFDQSLPLATFLAEPTIAGNAAALNVTDRRPVPVPLCRSGQERAWFVAPGANGDVLYLREFANAFGGLGSVYGLQSPGLQGERDPIDDMQSLARVHCDAIRYQQPQGPYYLAGHSFGGKLVFEVARLLEADGASVARLLMLDPAPPVDLSDRPEFRKSDRAWLEELLDLAEGFGSGAAQTEGRRRHRAESPPLESSDHELVLALGRALAEAGLVPQGADATFVRHLVAVYRTQQGMSYAPQGRLKAPLLVVRSTDDRAEVWSWPELEGIRDDPCAGWGDFVSRATGLEAPGNHLSMLRSANALRVAELVADWMSSDPSIVVRGGRTIASIKLLDRLPLTGGERMAQAGKNYK","length":7668,"molWeight":844473,"crc64":"A88CC234C1EB6769","md5":"BDBE0970A654DEA9935C7897B32465AD"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":2,"SUBCELLULAR LOCATION":1,"SIMILARITY":2},"countByFeatureType":{"Domain":8,"Region":5,"Compositional bias":6,"Modified residue":6},"uniParcId":"UPI001AF226B5"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TD84","uniProtkbId":"A0A8A4TD84_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":18,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47530.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"Fused isobutyryl-CoA mutase"}},"includes":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"Isobutyryl-CoA mutase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"ICM"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"5.4.99.13"}]}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"P-loop GTPase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"3.6.5.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"G-protein chaperone"}}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"icmF"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47530.1"}],"value":"J3U87_18200"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"Catalyzes the reversible interconversion of isobutyryl-CoA and n-butyryl-CoA, using radical chemistry. Also exhibits GTPase activity, associated with its G-protein domain (MeaI) that functions as a chaperone that assists cofactor delivery and proper holo-enzyme assembly"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2-methylpropanoyl-CoA = butanoyl-CoA","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13141"},{"database":"ChEBI","id":"CHEBI:57338"},{"database":"ChEBI","id":"CHEBI:57371"}],"ecNumber":"5.4.99.13","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"GTP + H2O = GDP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19669"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"adenosylcob(III)alamin","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001922"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18408"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"Is composed of four functional domains: the N-terminal 5'-deoxyadenosylcobalamin binding region that is homologous to the small subunit of ICM (IcmB), a middle P-loop GTPase domain (MeaI) that likely acts as a chaperone for ICM, a structured linker region involved in dimer formation, and a C-terminal part that is homologous to the large substrate-binding subunit of ICM (IcmA)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"Belongs to the IcmF family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"B12-binding","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51332"}]},{"type":"Binding site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18408"},{"database":"ChEBI","id":"CHEBI:27638"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"adenosylcob(III)alamin","id":"ChEBI:CHEBI:18408"},"ligandPart":{"name":"Co","id":"ChEBI:CHEBI:27638"}},{"type":"Binding site","location":{"start":{"value":205,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":209,"modifier":"EXACT"},"end":{"value":209,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":250,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":302,"modifier":"EXACT"},"end":{"value":302,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":348,"modifier":"EXACT"},"end":{"value":351,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":600,"modifier":"EXACT"},"end":{"value":600,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":635,"modifier":"EXACT"},"end":{"value":635,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":787,"modifier":"EXACT"},"end":{"value":787,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":831,"modifier":"EXACT"},"end":{"value":831,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":880,"modifier":"EXACT"},"end":{"value":880,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":915,"modifier":"EXACT"},"end":{"value":915,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":920,"modifier":"EXACT"},"end":{"value":920,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":1032,"modifier":"EXACT"},"end":{"value":1032,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":1152,"modifier":"EXACT"},"end":{"value":1152,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023186"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0143","category":"Molecular function","name":"Chaperone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022628"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0846","category":"Ligand","name":"Cobalamin"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023285"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0170","category":"Ligand","name":"Cobalt"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02050"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47530.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47530.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47530.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377199.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TD84","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_18200","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AACIRNY","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0031419","properties":[{"key":"GoTerm","value":"F:cobalamin binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0047727","properties":[{"key":"GoTerm","value":"F:isobutyryl-CoA mutase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0034784","properties":[{"key":"GoTerm","value":"F:pivalyl-CoA mutase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006637","properties":[{"key":"GoTerm","value":"P:acyl-CoA metabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd02071","properties":[{"key":"EntryName","value":"MM_CoA_mut_B12_BD"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.280:FF:000005","properties":[{"key":"EntryName","value":"Fused isobutyryl-CoA mutase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.280","properties":[{"key":"EntryName","value":"Cobalamin-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.240","properties":[{"key":"EntryName","value":"Methylmalonyl-CoA mutase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_02050","properties":[{"key":"EntryName","value":"IcmF"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006159","properties":[{"key":"EntryName","value":"Acid_CoA_mut_C"}]},{"database":"InterPro","id":"IPR016176","properties":[{"key":"EntryName","value":"Cbl-dep_enz_cat"}]},{"database":"InterPro","id":"IPR006158","properties":[{"key":"EntryName","value":"Cobalamin-bd"}]},{"database":"InterPro","id":"IPR036724","properties":[{"key":"EntryName","value":"Cobalamin-bd_sf"}]},{"database":"InterPro","id":"IPR052040","properties":[{"key":"EntryName","value":"GTPase/Isobutyryl-CoA_mutase"}]},{"database":"InterPro","id":"IPR033669","properties":[{"key":"EntryName","value":"IcmF"}]},{"database":"InterPro","id":"IPR006099","properties":[{"key":"EntryName","value":"MeMalonylCoA_mutase_a/b_cat"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"NCBIfam","id":"TIGR00640","properties":[{"key":"EntryName","value":"acid_CoA_mut_C"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43087:SF1","properties":[{"key":"EntryName","value":"LAO_AO TRANSPORT SYSTEM ATPASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43087","properties":[{"key":"EntryName","value":"LYSINE/ARGININE/ORNITHINE TRANSPORT SYSTEM KINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02310","properties":[{"key":"EntryName","value":"B12-binding"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03308","properties":[{"key":"EntryName","value":"MeaB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01642","properties":[{"key":"EntryName","value":"MM_CoA_mutase"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52242","properties":[{"key":"EntryName","value":"Cobalamin (vitamin B12)-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51703","properties":[{"key":"EntryName","value":"Cobalamin (vitamin B12)-dependent enzymes"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51332","properties":[{"key":"EntryName","value":"B12_BINDING"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTTPVAETAKGSQHAESGTRWKNKIRFVTAASLFDGHDASINIMRRILQSLGAEVIHLGHNRSVGEIVDAAIQEDAQGIAVSSYQGGHMEYFKFMYDLLRERGAPQIKLFGGGGGVIVPEEIKELHDYGVTRIFSPEDGAKMGLVGMIEYMLAECDFDPTHIPLGDSGTPVNRLARQITELELGRREGATSDKRVPVVGITGTGGAGKSSLTDEIVRRFIDHYADKKMAIISVDPSKRRTGGALLGDRIRMNAVACADPSGHPQVFMRSLATRASNRATSECLVSAVELLKEAEYDLIVVETAGIGQADSEIVDLVDFSVYVMTNEYGAATQLEKIDMLDLADMVVLNKFEKRGSEDAFRDIRKQFKRNRGWFDRDDDSLPIHATIASQFNDKGTNRMFHALVKMLNEKCGLSWPLPEMARMGIPEKAPIIPGERQRYLAEITETVRGYKAWAEAQAAIARTLYQLRGSIDSLRARMQQTDLNISAADGTGQVDLSPPDYQHAIDSLEQQYQYDQAKLDPRCRKLLEAFPAAREAYRGDELVTKIRDKEIRNTLFTTSLSGTKIPKVSLPRYQDWGDQLLWLLLENVPGSFPYTAGVFPFKRQGEDPTRMFAGEGTPERTNRRFHYVSKEQPAKRLSTAFDSVTLYGDDPARRPDIYGKIGNSGVSICNLDDMKKLYSGFDLCDPKTSVSMTINGPAPIILGFYMNAAIDQQIERHLQQQGRLEAVREEWYGRYDQKGLERPYYEGMAYDATSDRGFGLGLLGIPGVETVDPETYTKISTDVFRSVRGTVQADILKEDQAQNTCIFSTEFALKLMGDVQEFFIENGVRNYYSVSISGYHIAEAGANPISQAAFTLANGFTFVEYYLSRGMDINDFAPNLSFFFSNGIDPEYAVIGRVARRIWSVAMERRYGGNSRSQKLKYHIQTSGRSLHAQEIDFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEASVRRAMAIQLIINRELGLAKNENPLQGAFIIEELTELVEEAILSEFNRISERGGVLSAMETQYQRGKIQEESLYYEELKHNGKLPIIGVNTFLNPNAEDTAEGSLELIRSTEDEKEQQISYLANFQKFFADRCPDHLHRVKDAAIHHRNMFRPLLEATRYCSLGQISGLLYTVGGQYRRNM","length":1153,"molWeight":129180,"crc64":"E82D88430DF68117","md5":"37B09A71BC4C5F67CE7D73CD735A1322"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":2,"SUBUNIT":1,"DOMAIN":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Binding site":21},"uniParcId":"UPI001AFB6561"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TDQ4","uniProtkbId":"A0A8A4TDQ4_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":17,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47783.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"value":"Ribosomal protein uS12 methylthiotransferase RimO"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"value":"uS12 MTTase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"value":"uS12 methylthiotransferase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"value":"2.8.4.4"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"value":"Ribosomal protein uS12 (aspartate-C(3))-methylthiotransferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"value":"Ribosome maturation factor RimO"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47783.1"}],"value":"rimO"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47783.1"}],"value":"J3U87_19520"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"value":"Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein uS12"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-aspartate(89)-[ribosomal protein uS12]-hydrogen + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate(89)-[ribosomal protein uS12]-hydrogen + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:37087"},{"database":"Rhea","id":"RHEA-COMP:10460"},{"database":"Rhea","id":"RHEA-COMP:10461"},{"database":"Rhea","id":"RHEA-COMP:14737"},{"database":"Rhea","id":"RHEA-COMP:14739"},{"database":"ChEBI","id":"CHEBI:13193"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17319"},{"database":"ChEBI","id":"CHEBI:17499"},{"database":"ChEBI","id":"CHEBI:29917"},{"database":"ChEBI","id":"CHEBI:29961"},{"database":"ChEBI","id":"CHEBI:57844"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:64428"},{"database":"ChEBI","id":"CHEBI:73599"}],"ecNumber":"2.8.4.4","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"[4Fe-4S] cluster","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00780"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49883"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00780"}],"value":"Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00780"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"value":"Belongs to the methylthiotransferase family. RimO subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"MTTase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51449"}]},{"type":"Domain","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"Radical SAM core","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51918"}]},{"type":"Domain","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":450,"modifier":"EXACT"}},"description":"TRAM","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50926"}]},{"type":"Binding site","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00780"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"1"}},{"type":"Binding site","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00780"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"1"}},{"type":"Binding site","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00780"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"1"}},{"type":"Binding site","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01266"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","note":"4Fe-4S-S-AdoMet"}},{"type":"Binding site","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00780"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"2","note":"4Fe-4S-S-AdoMet"}},{"type":"Binding site","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01266"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","note":"4Fe-4S-S-AdoMet"}},{"type":"Binding site","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00780"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"2","note":"4Fe-4S-S-AdoMet"}},{"type":"Binding site","location":{"start":{"value":170,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01266"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","note":"4Fe-4S-S-AdoMet"}},{"type":"Binding site","location":{"start":{"value":170,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00780"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883","label":"2","note":"4Fe-4S-S-AdoMet"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022485"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"id":"KW-0004","category":"Ligand","name":"4Fe-4S"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023014"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47783.1"}],"id":"KW-0687","category":"Molecular function","name":"Ribonucleoprotein"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47783.1"}],"id":"KW-0689","category":"Molecular function","name":"Ribosomal protein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022691"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01865"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47783.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47783.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47783.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377449.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TDQ4","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_19520","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAKHYCR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0051539","properties":[{"key":"GoTerm","value":"F:4 iron, 4 sulfur cluster binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0035599","properties":[{"key":"GoTerm","value":"F:aspartic acid methylthiotransferase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0103039","properties":[{"key":"GoTerm","value":"F:protein methylthiotransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006400","properties":[{"key":"GoTerm","value":"P:tRNA modification"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd01335","properties":[{"key":"EntryName","value":"Radical_SAM"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.80.30.20:FF:000001","properties":[{"key":"EntryName","value":"tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.12160","properties":[{"key":"EntryName","value":"Methylthiotransferase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.140","properties":[{"key":"EntryName","value":"Nucleic acid-binding proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.80.30.20","properties":[{"key":"EntryName","value":"tm_1862 like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01865","properties":[{"key":"EntryName","value":"MTTase_RimO"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006638","properties":[{"key":"EntryName","value":"Elp3/MiaA/NifB-like_rSAM"}]},{"database":"InterPro","id":"IPR005839","properties":[{"key":"EntryName","value":"Methylthiotransferase"}]},{"database":"InterPro","id":"IPR020612","properties":[{"key":"EntryName","value":"Methylthiotransferase_CS"}]},{"database":"InterPro","id":"IPR013848","properties":[{"key":"EntryName","value":"Methylthiotransferase_N"}]},{"database":"InterPro","id":"IPR038135","properties":[{"key":"EntryName","value":"Methylthiotransferase_N_sf"}]},{"database":"InterPro","id":"IPR012340","properties":[{"key":"EntryName","value":"NA-bd_OB-fold"}]},{"database":"InterPro","id":"IPR005840","properties":[{"key":"EntryName","value":"Ribosomal_uS12_MeSTrfase_RimO"}]},{"database":"InterPro","id":"IPR007197","properties":[{"key":"EntryName","value":"rSAM"}]},{"database":"InterPro","id":"IPR023404","properties":[{"key":"EntryName","value":"rSAM_horseshoe"}]},{"database":"InterPro","id":"IPR058240","properties":[{"key":"EntryName","value":"rSAM_sf"}]},{"database":"InterPro","id":"IPR002792","properties":[{"key":"EntryName","value":"TRAM_dom"}]},{"database":"NCBIfam","id":"TIGR01125","properties":[{"key":"EntryName","value":"30S ribosomal protein S12 methylthiotransferase RimO"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00089","properties":[{"key":"EntryName","value":"MiaB/RimO family radical SAM methylthiotransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43837","properties":[{"key":"EntryName","value":"RIBOSOMAL PROTEIN S12 METHYLTHIOTRANSFERASE RIMO"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43837:SF1","properties":[{"key":"EntryName","value":"RIBOSOMAL PROTEIN US12 METHYLTHIOTRANSFERASE RIMO"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04055","properties":[{"key":"EntryName","value":"Radical_SAM"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18693","properties":[{"key":"EntryName","value":"TRAM_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00919","properties":[{"key":"EntryName","value":"UPF0004"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01082","properties":[{"key":"EntryName","value":"B12-binding_domain_containing"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDG01061","properties":[{"key":"EntryName","value":"methylthiotransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00029","properties":[{"key":"EntryName","value":"Radical_SAM"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDF00274","properties":[{"key":"EntryName","value":"ribosomal_protein_S12_methylth"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00729","properties":[{"key":"EntryName","value":"Elp3"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF102114","properties":[{"key":"EntryName","value":"Radical SAM enzymes"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51449","properties":[{"key":"EntryName","value":"MTTASE_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01278","properties":[{"key":"EntryName","value":"MTTASE_RADICAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51918","properties":[{"key":"EntryName","value":"RADICAL_SAM"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50926","properties":[{"key":"EntryName","value":"TRAM"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKKVGMLSLGCAKNLVDSEVMLGELAREGYALTNEADDAEIVIVNTCGFIESAKEESISAILDMAELKKQGKVKKLVVSGCLSQRYHTELAQDLPEVDLFLGINHVGGIQKFLTDLRGTSGDVVNREPTEAEDWRPDFLYNSEHPRVLTTPKFCAYVKISEGCDHVCSFCIIPKLRGKHRSRTIEDIVAEVRRLGDAGVREINLVAQDSTYYGRDLGLKDGLAQLMEAIAEVETIDWVRAHYMYPYQVTDRLLKLMAEHPKVCKYMDIPLQHADKHVLAAMKRGSGRPQLAKFLDRIREAVPDVWIRTSFIVGFPGEDERAFEELCAFVTEQEFDFIGVFTYSHEEDTTAYPLDDSISKEEKERRKAHLLALQKEISKKKLARHIGKTFDVLIEGVHPETELLLRGRHQGQAPEVDGEVLITEGFYQPGDIVPITIEETFDYDLAGRATGGVAV","length":454,"molWeight":50986,"crc64":"1A35B89E41963262","md5":"8074C3591A646CADBABD618A67FEEF5D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":3,"Binding site":9},"uniParcId":"UPI001AF78D3E"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TDS0","uniProtkbId":"A0A8A4TDS0_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":17,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48076.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"value":"Biosynthetic arginine decarboxylase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"value":"ADC"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"value":"4.1.1.19"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48076.1"}],"value":"speA"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48076.1"}],"value":"J3U87_21030"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00002257"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"value":"Catalyzes the biosynthesis of agmatine from arginine"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-arginine + H(+) = agmatine + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17641"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:32682"},{"database":"ChEBI","id":"CHEBI:58145"}],"ecNumber":"4.1.1.19","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"pyridoxal 5'-phosphate","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001933"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR001336-50"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:597326"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"value":"Amine and polyamine biosynthesis; agmatine biosynthesis; agmatine from L-arginine: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008357"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"value":"Belongs to the Orn/Lys/Arg decarboxylase class-II family. SpeA subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":342,"modifier":"EXACT"}},"description":"Orn/DAP/Arg decarboxylase 2 N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02784"}]},{"type":"Domain","location":{"start":{"value":373,"modifier":"EXACT"},"end":{"value":448,"modifier":"EXACT"}},"description":"Arginine decarboxylase helical bundle","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF17810"}]},{"type":"Domain","location":{"start":{"value":578,"modifier":"EXACT"},"end":{"value":630,"modifier":"EXACT"}},"description":"Arginine decarboxylase C-terminal helical","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF17944"}]},{"type":"Active site","location":{"start":{"value":499,"modifier":"EXACT"},"end":{"value":499,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR600183-50"}]},{"type":"Binding site","location":{"start":{"value":282,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"ligand":{"name":"substrate"}},{"type":"Modified residue","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR001336-50"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022793"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"id":"KW-0210","category":"Molecular function","name":"Decarboxylase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023115"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"id":"KW-0620","category":"Biological process","name":"Polyamine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022898"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"id":"KW-0663","category":"Ligand","name":"Pyridoxal phosphate"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023066"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01417"}],"id":"KW-0745","category":"Biological process","name":"Spermidine biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48076.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48076.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD48076.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377738.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TDS0","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_21030","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAENIYW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00186","properties":[{"key":"RectionId","value":"UER00284"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0008792","properties":[{"key":"GoTerm","value":"F:arginine decarboxylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006527","properties":[{"key":"GoTerm","value":"P:L-arginine catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008295","properties":[{"key":"GoTerm","value":"P:spermidine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd06830","properties":[{"key":"EntryName","value":"PLPDE_III_ADC"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.287.3440","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.58.930","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.10","properties":[{"key":"EntryName","value":"Alanine racemase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.37.10","properties":[{"key":"EntryName","value":"Lyase, Ornithine Decarboxylase, Chain A, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01417","properties":[{"key":"EntryName","value":"SpeA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR009006","properties":[{"key":"EntryName","value":"Ala_racemase/Decarboxylase_C"}]},{"database":"InterPro","id":"IPR040634","properties":[{"key":"EntryName","value":"Arg_decarb_HB"}]},{"database":"InterPro","id":"IPR041128","properties":[{"key":"EntryName","value":"Arg_decarbox_C"}]},{"database":"InterPro","id":"IPR002985","properties":[{"key":"EntryName","value":"Arg_decrbxlase"}]},{"database":"InterPro","id":"IPR022657","properties":[{"key":"EntryName","value":"De-COase2_CS"}]},{"database":"InterPro","id":"IPR022644","properties":[{"key":"EntryName","value":"De-COase2_N"}]},{"database":"InterPro","id":"IPR022653","properties":[{"key":"EntryName","value":"De-COase2_pyr-phos_BS"}]},{"database":"InterPro","id":"IPR000183","properties":[{"key":"EntryName","value":"Orn/DAP/Arg_de-COase"}]},{"database":"InterPro","id":"IPR029066","properties":[{"key":"EntryName","value":"PLP-binding_barrel"}]},{"database":"NCBIfam","id":"NF003763","properties":[{"key":"EntryName","value":"speA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01273","properties":[{"key":"EntryName","value":"speA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43295","properties":[{"key":"EntryName","value":"ARGININE DECARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43295:SF9","properties":[{"key":"EntryName","value":"BIOSYNTHETIC ARGININE DECARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17810","properties":[{"key":"EntryName","value":"Arg_decarb_HB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17944","properties":[{"key":"EntryName","value":"Arg_decarbox_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02784","properties":[{"key":"EntryName","value":"Orn_Arg_deC_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001336","properties":[{"key":"EntryName","value":"Arg_decrbxlase"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01180","properties":[{"key":"EntryName","value":"ARGDCRBXLASE"}]},{"database":"PRINTS","id":"PR01179","properties":[{"key":"EntryName","value":"ODADCRBXLASE"}]},{"database":"SUPFAM","id":"SSF50621","properties":[{"key":"EntryName","value":"Alanine racemase C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51419","properties":[{"key":"EntryName","value":"PLP-binding barrel"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00878","properties":[{"key":"EntryName","value":"ODR_DC_2_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00879","properties":[{"key":"EntryName","value":"ODR_DC_2_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTNWSLKKANEVYKVNQWGLGYFAINESGNVCVRPSADSEHKVDLKKLVDELRQRKINVPVLIRFMDILRDRIAQISTAFKNAIVENEYQGQYYPLFPIKVNQERDVVSSMLEYGKEFGLGLEAGSKAELLIVLALTLDPDTPIVCNGYKDREFVQLVGMAHKMGKKIFPVIENYDELETFIAHYKETGIMPRLGMRIKLSTKGVGKWAKTGGDASKFGLRIPEVLKAVERLREENLLDSLNLLHFHIGSQITKIKVVKQAIVETLRVFAEIVRLGAKLEYLDIGGGLGVDYDGSSQETYSTINYTLQEYASDIVYRIQQICQEKQIAVPNIFSESGRFLAAHYSMLITNVPTVSSIGPCGLEFPHPDKGYGPVKEMYDLLEGLTPANSQESFHDAVQYRSEALSLFNLGYLTLPERAYMEELYWRIMRKVYEQAQIQGLLSPEMEDLEQELSDTYFANFSLFQSLPDSWAIDQIFPIIPIHRLNEEPDRIATVVDLTCDSDGVIDDYIGEDGPRHCVPLHSVHNGNNYYIGIFMIGAYQETLGELHNLFGDTHAVQIRIQGENQYKIESLIKGDTIHQVLTYVSFNRKELLHKMREQIEQAVDERKLKLDESAQMMDLYEEGLYGYTYFEE","length":632,"molWeight":72122,"crc64":"3D7E5817C0988BEC","md5":"4E716E16A87BC474A915F847C65F8EDF"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":3,"Active site":1,"Binding site":1,"Modified residue":1},"uniParcId":"UPI001AF38FAD"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TDS7","uniProtkbId":"A0A8A4TDS7_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":17,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47803.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Polyamine aminopropyltransferase"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Putrescine aminopropyltransferase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"PAPT"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Spermidine synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"SPDS"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"SPDSY"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"2.5.1.16"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"speE"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47803.1"}],"value":"J3U87_19620"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:12721"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17509"},{"database":"ChEBI","id":"CHEBI:57443"},{"database":"ChEBI","id":"CHEBI:57834"},{"database":"ChEBI","id":"CHEBI:326268"}],"ecNumber":"2.5.1.16","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Homodimer or homotetramer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007867"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Belongs to the spermidine/spermine synthase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":40,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}]},{"type":"Transmembrane","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}]},{"type":"Transmembrane","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":122,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}]},{"type":"Transmembrane","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":151,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}]},{"type":"Transmembrane","location":{"start":{"value":172,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}]},{"type":"Transmembrane","location":{"start":{"value":197,"modifier":"EXACT"},"end":{"value":214,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}]},{"type":"Transmembrane","location":{"start":{"value":226,"modifier":"EXACT"},"end":{"value":248,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}]},{"type":"Domain","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":472,"modifier":"EXACT"}},"description":"PABS","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51006"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":393,"modifier":"EXACT"},"end":{"value":393,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00354"}]},{"type":"Binding site","location":{"start":{"value":267,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17509"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"ligand":{"name":"S-methyl-5'-thioadenosine","id":"ChEBI:CHEBI:17509"}},{"type":"Binding site","location":{"start":{"value":297,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57834"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"ligand":{"name":"spermidine","id":"ChEBI:CHEBI:57834"}},{"type":"Binding site","location":{"start":{"value":321,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57834"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"ligand":{"name":"spermidine","id":"ChEBI:CHEBI:57834"}},{"type":"Binding site","location":{"start":{"value":341,"modifier":"EXACT"},"end":{"value":341,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17509"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"ligand":{"name":"S-methyl-5'-thioadenosine","id":"ChEBI:CHEBI:17509"}},{"type":"Binding site","location":{"start":{"value":375,"modifier":"EXACT"},"end":{"value":376,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17509"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"ligand":{"name":"S-methyl-5'-thioadenosine","id":"ChEBI:CHEBI:17509"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023115"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"id":"KW-0620","category":"Biological process","name":"Polyamine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023066"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"id":"KW-0745","category":"Biological process","name":"Spermidine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00198"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47803.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47803.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47803.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377469.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TDS7","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_19620","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ACIDKTV","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00248","properties":[{"key":"RectionId","value":"UER00314"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0004766","properties":[{"key":"GoTerm","value":"F:spermidine synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0010487","properties":[{"key":"GoTerm","value":"F:thermospermine synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0008295","properties":[{"key":"GoTerm","value":"P:spermidine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd02440","properties":[{"key":"EntryName","value":"AdoMet_MTases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.150","properties":[{"key":"EntryName","value":"Vaccinia Virus protein VP39"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00198","properties":[{"key":"EntryName","value":"Spermidine_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036259","properties":[{"key":"EntryName","value":"MFS_trans_sf"}]},{"database":"InterPro","id":"IPR030374","properties":[{"key":"EntryName","value":"PABS"}]},{"database":"InterPro","id":"IPR030373","properties":[{"key":"EntryName","value":"PABS_CS"}]},{"database":"InterPro","id":"IPR029063","properties":[{"key":"EntryName","value":"SAM-dependent_MTases_sf"}]},{"database":"InterPro","id":"IPR001045","properties":[{"key":"EntryName","value":"Spermi_synthase"}]},{"database":"NCBIfam","id":"NF037959","properties":[{"key":"EntryName","value":"MFS_SpdSyn"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF002956","properties":[{"key":"EntryName","value":"PRK03612.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43317","properties":[{"key":"EntryName","value":"THERMOSPERMINE SYNTHASE ACAULIS5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43317:SF1","properties":[{"key":"EntryName","value":"THERMOSPERMINE SYNTHASE ACAULIS5"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01564","properties":[{"key":"EntryName","value":"Spermine_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103473","properties":[{"key":"EntryName","value":"MFS general substrate transporter"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53335","properties":[{"key":"EntryName","value":"S-adenosyl-L-methionine-dependent methyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01330","properties":[{"key":"EntryName","value":"PABS_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51006","properties":[{"key":"EntryName","value":"PABS_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTSLPASNPAIPQTPDPSTSPTGDAVAARQHRLRGRMLKLCIFATGFSGIVAEYAMSTLASYLLGNSVKQWTLIISLMLFAMGLGSRFSKHLNRRLLTWFVLAETILSLLCAASATFIYFIAAFTESLAVMIYSVAMAIGFLIGMEMPLATRINEEFEELRYNISTILEKDYYGALLGGLLFAFVALPYLGLTYTPIILGMINLAAAIGLTAVFKDRLAGRQNWYLAFFATGTSLLLLALFAKPIVLFGEQARYRDKVIYQEQTPYQRIVLTRWKQDHWLYLNGHQQFSSYDEAHYHEPLVHPAMALSQDRRRVLILGGGDGLAARELLKYPEVEDIVLVDLDPAVTRLGREHPVLRELNGGALDDPRVQVVNRDAYLFLEENRMPFGVIIVDLPDPKTVDLARLYSVQFYRLAKRNLITGGTLITQATSPFFSRQAFTCIYKTMGAADLQTTTFHNHIPTMGEWGWVLGWNLPAAPPRSMRELLSDQRFDTIDTRFLNRDAMISMSHFGKGILEDLDEITVNSETDDLSLHRYYAKGRWDFY","length":543,"molWeight":61233,"crc64":"3B3EE0C16442620E","md5":"27CC129DF8D8F360DF4B49D6E267AACB"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Transmembrane":7,"Domain":1,"Region":1,"Active site":1,"Binding site":5},"uniParcId":"UPI001AFAB583"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TDU1","uniProtkbId":"A0A8A4TDU1_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":20,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48096.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00071867"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"value":"Probable tRNA sulfurtransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00066827"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"value":"2.8.1.4"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00075337"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"value":"Sulfur carrier protein ThiS sulfurtransferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00077849"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"value":"Thiamine biosynthesis protein ThiI"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00080570"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"value":"tRNA 4-thiouridine synthase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48096.1"}],"value":"thiI"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48096.1"}],"value":"J3U87_21130"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00058382"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"value":"Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + ATP + H(+) = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + 2 oxidized [2Fe-2S]-[ferredoxin] + AMP + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24176"},{"database":"Rhea","id":"RHEA-COMP:10000"},{"database":"Rhea","id":"RHEA-COMP:10001"},{"database":"Rhea","id":"RHEA-COMP:13337"},{"database":"Rhea","id":"RHEA-COMP:13338"},{"database":"Rhea","id":"RHEA-COMP:13339"},{"database":"Rhea","id":"RHEA-COMP:13340"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29950"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:33737"},{"database":"ChEBI","id":"CHEBI:33738"},{"database":"ChEBI","id":"CHEBI:61963"},{"database":"ChEBI","id":"CHEBI:65315"},{"database":"ChEBI","id":"CHEBI:136798"},{"database":"ChEBI","id":"CHEBI:456215"}],"ecNumber":"2.8.1.4","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00050570"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"[ThiS sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [ThiS sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:43340"},{"database":"Rhea","id":"RHEA-COMP:12157"},{"database":"Rhea","id":"RHEA-COMP:12158"},{"database":"Rhea","id":"RHEA-COMP:12910"},{"database":"Rhea","id":"RHEA-COMP:19908"},{"database":"ChEBI","id":"CHEBI:13193"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17499"},{"database":"ChEBI","id":"CHEBI:29950"},{"database":"ChEBI","id":"CHEBI:61963"},{"database":"ChEBI","id":"CHEBI:90618"},{"database":"ChEBI","id":"CHEBI:232372"},{"database":"ChEBI","id":"CHEBI:456215"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052330"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"value":"Cofactor biosynthesis; thiamine diphosphate biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00061472"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"value":"Belongs to the ThiI family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"THUMP","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51165"}]},{"type":"Binding site","location":{"start":{"value":183,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":209,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":287,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":296,"modifier":"EXACT"},"end":{"value":296,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022884"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022977"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"id":"KW-0784","category":"Biological process","name":"Thiamine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022555"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00021"}],"id":"KW-0820","category":"Molecular function","name":"tRNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48096.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD48096.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD48096.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377757.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TDU1","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_21130","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAHLMMK","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"UniPathway","id":"UPA00060","properties":[{"key":"RectionId","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004810","properties":[{"key":"GoTerm","value":"F:CCA tRNA nucleotidyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0000049","properties":[{"key":"GoTerm","value":"F:tRNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0140741","properties":[{"key":"GoTerm","value":"F:tRNA-uracil-4 sulfurtransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0009229","properties":[{"key":"GoTerm","value":"P:thiamine diphosphate biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0052837","properties":[{"key":"GoTerm","value":"P:thiazole biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0002937","properties":[{"key":"GoTerm","value":"P:tRNA 4-thiouridine biosynthesis"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd01712","properties":[{"key":"EntryName","value":"PPase_ThiI"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd11716","properties":[{"key":"EntryName","value":"THUMP_ThiI"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.620:FF:000053","properties":[{"key":"EntryName","value":"Probable tRNA sulfurtransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.2130.30","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.620","properties":[{"key":"EntryName","value":"HUPs"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00021","properties":[{"key":"EntryName","value":"ThiI"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR014729","properties":[{"key":"EntryName","value":"Rossmann-like_a/b/a_fold"}]},{"database":"InterPro","id":"IPR020536","properties":[{"key":"EntryName","value":"ThiI_AANH"}]},{"database":"InterPro","id":"IPR054173","properties":[{"key":"EntryName","value":"ThiI_fer"}]},{"database":"InterPro","id":"IPR049961","properties":[{"key":"EntryName","value":"ThiI_N"}]},{"database":"InterPro","id":"IPR004114","properties":[{"key":"EntryName","value":"THUMP_dom"}]},{"database":"InterPro","id":"IPR049962","properties":[{"key":"EntryName","value":"THUMP_ThiI"}]},{"database":"InterPro","id":"IPR003720","properties":[{"key":"EntryName","value":"tRNA_STrfase"}]},{"database":"NCBIfam","id":"TIGR00342","properties":[{"key":"EntryName","value":"tRNA uracil 4-sulfurtransferase ThiI"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43209","properties":[{"key":"EntryName","value":"TRNA SULFURTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43209:SF1","properties":[{"key":"EntryName","value":"TRNA SULFURTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02568","properties":[{"key":"EntryName","value":"ThiI"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22025","properties":[{"key":"EntryName","value":"ThiI_fer"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02926","properties":[{"key":"EntryName","value":"THUMP"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00981","properties":[{"key":"EntryName","value":"THUMP"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52402","properties":[{"key":"EntryName","value":"Adenine nucleotide alpha hydrolases-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF143437","properties":[{"key":"EntryName","value":"THUMP domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51165","properties":[{"key":"EntryName","value":"THUMP"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSDKPSAYVLRFGELALKGRNQDRFVDDLIKIVKPRVKHLGGKLEKRHKKVLLRTEAEPEDVRKALSTVFGLSGISPIWIVGHDIEVIKEKAWELMAPHCNSDKTFAVRGRRANKRYPMNSMDLQLELAGFLLDQGLDLKVDLKHPDLPLTVSIELKETWLYLDTWPGLGGLPVNGKTKHGLLLSGGIDSPVAGHLIQKRGGSIDAIYFHTPPFTVEAAREKVVDLANVLARYQNGLTLYVVNFTALMKTLRAECLDKYMVVLTRRFMMRVAVALLEKVNGQSIVTGESLGQVASQTIENITAINEGVPLPILRPLIGMDKTEIIRISEHMSAYPISIQPFEDCCSLFSPKEPVTRASLKVVHREERKLDIEALVAEAVEHTEALAIEARFPIAEST","length":397,"molWeight":44253,"crc64":"B3A909ABE64B2064","md5":"6EED70749D993A043DAB60A45DA02C85"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Binding site":5},"uniParcId":"UPI001AF47800"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A8A4TDU8","uniProtkbId":"A0A8A4TDU8_SULCO","entryAudit":{"firstPublicDate":"2022-05-25","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2022-05-25","entryVersion":15,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Sulfidibacter corallicola","taxonId":2818388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47823.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"lineage":["Bacteria","Pseudomonadati","Acidobacteriota","Holophagae","Acanthopleuribacterales","Acanthopleuribacteraceae","Sulfidibacter"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Protein-glutamate methylesterase/protein-glutamine glutaminase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"3.1.1.61"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"3.5.1.44"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47823.1"}],"value":"cheB"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47823.1"}],"value":"J3U87_19725"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-glutaminyl-[protein] + H2O = L-glutamyl-[protein] + NH4(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16441"},{"database":"Rhea","id":"RHEA-COMP:10207"},{"database":"Rhea","id":"RHEA-COMP:10208"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:29973"},{"database":"ChEBI","id":"CHEBI:30011"}],"ecNumber":"3.5.1.44","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"[protein]-L-glutamate 5-O-methyl ester + H2O = L-glutamyl-[protein] + methanol + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:23236"},{"database":"Rhea","id":"RHEA-COMP:10208"},{"database":"Rhea","id":"RHEA-COMP:10311"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17790"},{"database":"ChEBI","id":"CHEBI:29973"},{"database":"ChEBI","id":"CHEBI:82795"}],"ecNumber":"3.1.1.61","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048267"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"value":"Belongs to the CheB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":121,"modifier":"EXACT"}},"description":"Response regulatory","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50110"}]},{"type":"Domain","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"CheB-type methylesterase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50122"}]},{"type":"Region","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":181,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00050"}]},{"type":"Active site","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00050"}]},{"type":"Active site","location":{"start":{"value":321,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00050"}]},{"type":"Modified residue","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"4-aspartylphosphate","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00169"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022500"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"id":"KW-0145","category":"Biological process","name":"Chemotaxis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47823.1"}],"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00099"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00169"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000663929"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47823.1"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-77JE887VULTLB","citationType":"submission","authors":["Wang G."],"title":"Acanthopleuribacteraceae sp. M133.","publicationDate":"MAR-2021","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47823.1"}],"value":"M133","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"QTD47823.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP071793","properties":[{"key":"ProteinId","value":"QTD47823.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_237377490.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP071793.1"}]},{"database":"AlphaFoldDB","id":"A0A8A4TDU8","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"scor:J3U87_19725","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AEHNQPL","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"Proteomes","id":"UP000663929","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000156","properties":[{"key":"GoTerm","value":"F:phosphorelay response regulator activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008984","properties":[{"key":"GoTerm","value":"F:protein-glutamate methylesterase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0050568","properties":[{"key":"GoTerm","value":"F:protein-glutamine glutaminase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006935","properties":[{"key":"GoTerm","value":"P:chemotaxis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd16432","properties":[{"key":"EntryName","value":"CheB_Rec"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd17541","properties":[{"key":"EntryName","value":"REC_CheB-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.2300","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.180","properties":[{"key":"EntryName","value":"Methylesterase CheB, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00099","properties":[{"key":"EntryName","value":"CheB_chemtxs"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008248","properties":[{"key":"EntryName","value":"CheB-like"}]},{"database":"InterPro","id":"IPR035909","properties":[{"key":"EntryName","value":"CheB_C"}]},{"database":"InterPro","id":"IPR011006","properties":[{"key":"EntryName","value":"CheY-like_sf"}]},{"database":"InterPro","id":"IPR000673","properties":[{"key":"EntryName","value":"Sig_transdc_resp-reg_Me-estase"}]},{"database":"InterPro","id":"IPR001789","properties":[{"key":"EntryName","value":"Sig_transdc_resp-reg_receiver"}]},{"database":"NCBIfam","id":"NF001965","properties":[{"key":"EntryName","value":"cheB"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42872","properties":[{"key":"EntryName","value":"PROTEIN-GLUTAMATE METHYLESTERASE/PROTEIN-GLUTAMINE GLUTAMINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42872:SF6","properties":[{"key":"EntryName","value":"PROTEIN-GLUTAMATE METHYLESTERASE_PROTEIN-GLUTAMINE GLUTAMINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01339","properties":[{"key":"EntryName","value":"CheB_methylest"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00072","properties":[{"key":"EntryName","value":"Response_reg"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000876","properties":[{"key":"EntryName","value":"RR_chemtxs_CheB"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00448","properties":[{"key":"EntryName","value":"REC"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52172","properties":[{"key":"EntryName","value":"CheY-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52738","properties":[{"key":"EntryName","value":"Methylesterase CheB, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50122","properties":[{"key":"EntryName","value":"CHEB"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50110","properties":[{"key":"EntryName","value":"RESPONSE_REGULATORY"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPRVLIVEDMATYRTIIARSLAEIEDVEVVGRVSNGKKALDFLERNEVDLITLDVEMPVMDGLATLKELRKRRMHIDVVMLSGLTDQAAQLTIKCLELGALDFVLKPATSSFVENQKILVESLRQFLNNLKRRLSFRAPPRVTPRSPITRFGTPRRDTAGRPEVSPPPPAPTPKPTAPRRTRLVLPRPKIVLIGVSTGGPKALSEVFETLKGPLPFPILIVQHMPPKFTKSLADSLNKKTDIDVVEAMDGDVVKSNTAYIAPGGYHMVVMNDEDFGFILATNQNPPVNSCRPSVDVLFNSVVPFFKRGDIAAIVMTGMGRDGADSCEVLHKSGHYIASQSAETCTIYGMPKAVEDLGIQDEVIDLDQIAPFIMRLGKQTGTLGAKLWQSGSGA","length":393,"molWeight":43035,"crc64":"9C39DF2FAF287190","md5":"412BEA03FA4A3C8B720DF71A0BFB9931"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Region":1,"Compositional bias":1,"Active site":3,"Modified residue":1},"uniParcId":"UPI001AF0FE98"}}]}