{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A510J9W8","uniProtkbId":"A0A510J9W8_9MAGN","entryAudit":{"firstPublicDate":"2019-10-16","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2019-10-16","entryVersion":25,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Clematis patens","taxonId":231656,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BBM28223.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","Ranunculales","Ranunculaceae","Ranunculoideae","Anemoneae","Clematis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00066564"}],"value":"flavonoid 3',5'-hydroxylase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00066564"}],"value":"1.14.14.81"}]}},"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2R,3R)-dihydrokaempferol + 2 reduced [NADPH--hemoprotein reductase] + 2 O2 = (2R,3R)-dihydromyricetin + 2 oxidized [NADPH--hemoprotein reductase] + 2 H2O + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:61108"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15401"},{"database":"ChEBI","id":"CHEBI:28429"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095513"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:61109"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095513"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2R,3R)-dihydrokaempferol + reduced [NADPH--hemoprotein reductase] + O2 = (2R,3R)-dihydroquercetin + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:61112"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15401"},{"database":"ChEBI","id":"CHEBI:17948"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095571"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:61113"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095571"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2R,3R)-dihydroquercetin + reduced [NADPH--hemoprotein reductase] + O2 = (2R,3R)-dihydromyricetin + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:61116"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:17948"},{"database":"ChEBI","id":"CHEBI:28429"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095574"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:61117"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095574"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2S)-naringenin + 2 reduced [NADPH--hemoprotein reductase] + 2 O2 = (2S)-dihydrotricetin + 2 oxidized [NADPH--hemoprotein reductase] + 2 H2O + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:61104"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:17846"},{"database":"ChEBI","id":"CHEBI:48026"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095488"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:61105"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095488"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2S)-naringenin + reduced [NADPH--hemoprotein reductase] + O2 = (S)-eriodictyol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:61096"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:17846"},{"database":"ChEBI","id":"CHEBI:28412"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095490"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:61097"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095490"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(S)-eriodictyol + reduced [NADPH--hemoprotein reductase] + O2 = (2S)-dihydrotricetin + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:61100"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:28412"},{"database":"ChEBI","id":"CHEBI:48026"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095687"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:61101"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095687"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 3',5'-unsubstituted flavanone + 2 reduced [NADPH--hemoprotein reductase] + 2 O2 = a 3',5'-dihydroxyflavanone + 2 oxidized [NADPH--hemoprotein reductase] + 2 H2O + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:55448"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:48025"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:138897"}],"ecNumber":"1.14.14.81","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095810"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:55449"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095810"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 3',5'-unsubstituted flavanone + reduced [NADPH--hemoprotein reductase] + O2 = a 3'-hydroxy,5'-unsubstituted flavanone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10592"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:138897"},{"database":"ChEBI","id":"CHEBI:138951"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095623"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:10593"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095623"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 3'-hydroxy,5'-unsubstituted flavanone + reduced [NADPH--hemoprotein reductase] + O2 = a 3',5'-dihydroxyflavanone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18797"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:48025"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:138951"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095468"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:18798"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00095468"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"heme","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001971"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602401-1"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:30413"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004935"}],"value":"Pigment biosynthesis; anthocyanin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004167"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004167"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010617"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000461"}],"value":"Belongs to the cytochrome P450 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Binding site","location":{"start":{"value":448,"modifier":"EXACT"},"end":{"value":448,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30413"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602401-1"}],"ligand":{"name":"heme","id":"ChEBI:CHEBI:30413"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022617"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602401-1"}],"id":"KW-0349","category":"Ligand","name":"Heme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602401-1"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602401-1"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023033"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000461"}],"id":"KW-0503","category":"Molecular function","name":"Monooxygenase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000461"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-F0QA4VPQBFQ8Q","citationType":"submission","authors":["Kim S.Y."],"title":"Isolation and expression pattern of flavonoid 3'5'-hydroxylase in Clematis patens.","publicationDate":"JUL-2016","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BBM28223.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LC169756","properties":[{"key":"ProteinId","value":"BBM28223.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"A0A510J9W8","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A510J9W8","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0033772","properties":[{"key":"GoTerm","value":"F:flavonoid 3',5'-hydroxylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0020037","properties":[{"key":"GoTerm","value":"F:heme binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0044550","properties":[{"key":"GoTerm","value":"P:secondary metabolite biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"CDD","id":"cd20657","properties":[{"key":"EntryName","value":"CYP75"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.630.10:FF:000111","properties":[{"key":"EntryName","value":"Flavonoid 3',5'-hydroxylase 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.630.10","properties":[{"key":"EntryName","value":"Cytochrome P450"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001128","properties":[{"key":"EntryName","value":"Cyt_P450"}]},{"database":"InterPro","id":"IPR017972","properties":[{"key":"EntryName","value":"Cyt_P450_CS"}]},{"database":"InterPro","id":"IPR002401","properties":[{"key":"EntryName","value":"Cyt_P450_E_grp-I"}]},{"database":"InterPro","id":"IPR036396","properties":[{"key":"EntryName","value":"Cyt_P450_sf"}]},{"database":"PANTHER","id":"PTHR47944","properties":[{"key":"EntryName","value":"CYTOCHROME P450 98A9"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47944:SF18","properties":[{"key":"EntryName","value":"FLAVONOID 3'-MONOOXYGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00067","properties":[{"key":"EntryName","value":"p450"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00463","properties":[{"key":"EntryName","value":"EP450I"}]},{"database":"PRINTS","id":"PR00385","properties":[{"key":"EntryName","value":"P450"}]},{"database":"SUPFAM","id":"SSF48264","properties":[{"key":"EntryName","value":"Cytochrome P450"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00086","properties":[{"key":"EntryName","value":"CYTOCHROME_P450"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVINMFVLRELSYAFILFFITHIFGRSLLSHYARKLPPGPTGFPVVGSLPFLGSMPHVALAEMSKKYGPAMYLKLGSRGMVVASTPDSARAFLKTLDLNFSNRPTDSGATHMAYDSQDMVFADYGPRWKLLRRVSNLHMLGGKALEDWTNVRKNEVGHMLQAMHESSCRGEPVVVADMLVYAMANMIGQVILSRRVFVTKGTELNEFKEMVVELMTSAGLFNVGDYIPSIAWMDLQGIVRGMKSLHNKFDVLLNKMLKEHESTRHERKEKPDLLDVLLENRDNKAVGEERITDTNIKALLLNLFTAGTDTSSSSIEWALAEMMKNPSIFKRAHVEMDHVIGNNRRLEESDIPNLPYLQAICKETFRKHPSTPLNLPREAVESCEVNGYYIPKGTRLSVNIWGMGRDPNVWENPLEFNPDRFLRVETMKIDPRGNHFELIPFGAGRRICAGSRMAIVLVEYMLGTLVHSFDWNLADGVELNMDESFGLALQKAVPLVAIVTPRLPPSVYYV","length":510,"molWeight":57509,"crc64":"A7CBF2EE5D6F76FA","md5":"6A8C1CFC1924C28E32CF0E10CBD4AA6E"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":9,"COFACTOR":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Binding site":1},"uniParcId":"UPI00116501EE"}}]}