{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8KQL6","uniProtkbId":"PADGT_ACHLA","entryAudit":{"firstPublicDate":"2014-02-19","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2002-10-01","entryVersion":71,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Acholeplasma laidlawii","taxonId":2148,"lineage":["Bacteria","Bacillati","Mycoplasmatota","Mollicutes","Acholeplasmatales","Acholeplasmataceae","Acholeplasma"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Processive diacylglycerol alpha-glucosyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10220338"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12464611"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1533160"}],"value":"2.4.1.208"},{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10220338"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12464611"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1533160"}],"value":"2.4.1.337"}]},"alternativeNames":[{"fullName":{"value":"Alpha-diglucosyldiacylglycerol synthase"},"shortNames":[{"value":"Alpha-DGS"},{"value":"DGlcDAG synthase"}]},{"fullName":{"value":"Alpha-monoglucosyldiacylglycerol synthase"},"shortNames":[{"value":"Alpha-MGS"},{"value":"MGlcDAG synthase"}]},{"fullName":{"value":"Glucosyl-alpha-1,2-glucosyldiacylglycerol synthase"}},{"fullName":{"value":"UDP-glucose:1,2-diacylglycerol 3-alpha-D-glucosyltransferase"}}]},"genes":[{"geneName":{"value":"dgs"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10220338"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12464611"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1533160"}],"value":"Processive glucosyltransferase involved in the biosynthesis of both the non-bilayer-prone alpha-monoglucosyldiacylglycerol and the bilayer-forming membrane lipid alpha-diglucosyldiacylglycerol. 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Inhibited by 1,2-diacyl-3-O-(alpha-D-galactopyranosyl)-sn-glycerol, 1,2-diacyl-3-O-[6-O-acyl(alpha-D-glucopyranosyl)]-sn-glycerol and 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->2)-O-(6-O-acyl-alpha-D-glucopyranosyl)]-sn-glycerol"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":19.0,"unit":"nmol/h/mg","enzyme":"enzyme (alpha-diglucosyldiacylglycerol synthase activity at 28 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10220338"}]}],"michaelisConstants":[{"constant":0.14,"unit":"mM","substrate":"UDP-Glc (alpha-diglucosyldiacylglycerol synthase activity at 28 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10220338"}]}]}},{"texts":[{"value":"Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12464611"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1533160"}],"value":"Cell membrane","id":"SL-0039"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase group 1 family. 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This is a major component for maintaining a certain anionic lipid surface charge density, for balancing the bilayer to non-bilayer phase equilibria and for keeping a constant lipid bilayer spontaneous curvature (curvature packing stress). Catalyzes the transfer of a glucosyl residue from UDP-Glc to diacylglycerol (DAG) acceptor to form the corresponding alpha-glucosyl-DAG (1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol). 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