{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0DYK9","uniProtkbId":"APTM1_ANTMM","entryAudit":{"firstPublicDate":"2026-09-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2026-09-02","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Anthracobia melaloma","commonName":"Charcoal eyelash","synonyms":["Peziza melaloma"],"taxonId":2074765,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Pezizomycetes","Pezizales","Pyronemataceae","Anthracobia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15110094"}],"value":"Pyranoid enolone tautomerase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15110094"}],"value":"5.3.2.7"}]},"flag":"Fragments"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15110094"}],"value":"APTM1"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15110094"}],"value":"Pyranoid enolone tautomerase that acts within the anhydrofructose pathway of glycogen degradation via 1,5-anhydro-d-fructose (PubMed:15110094). APTM1 catalyzes the last step of the pathway which is the tautomerization of ascopyrone M (APP) to yield ascopyrone P (APP) (PubMed:15110094)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ascopyrone M = ascopyrone P","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22568"},{"database":"ChEBI","id":"CHEBI:50070"},{"database":"ChEBI","id":"CHEBI:50071"}],"ecNumber":"5.3.2.7","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15110094"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:22569"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15110094"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16302767"}],"value":"Ascopyrone P (APP) acts as an antibrowning agent comparable to kojic acid. It shows an antibrowning effect in a range of agricultural products, such as various cultivars of apple, pear, potato, lettuce, and varieties of green tea in an efficacy concentration range from 300 to 500 ppm. Mechanism studies indicate that, like kojic acid, APP shows inhibition toward plant polyphenol oxidase and was able to decolor quinones"}],"commentType":"BIOTECHNOLOGY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"Pyranoid enolone tautomerase","featureId":"PRO_0000464856"},{"type":"Non-adjacent residues","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Non-adjacent residues","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Non-adjacent residues","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Non-adjacent residues","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Non-adjacent residues","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Non-adjacent residues","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Non-adjacent residues","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":""},{"type":"Non-terminal residue","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":""}],"keywords":[{"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0321","category":"Biological process","name":"Glycogen metabolism"},{"id":"KW-0413","category":"Molecular function","name":"Isomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"15110094","citationType":"journal article","authors":["Yu S.","Refdahl C.","Lundt I."],"citationCrossReferences":[{"database":"PubMed","id":"15110094"},{"database":"DOI","id":"10.1016/j.bbagen.2004.03.004"}],"title":"Enzymatic description of the anhydrofructose pathway of glycogen degradation; I. Identification and purification of anhydrofructose dehydratase, ascopyrone tautomerase and alpha-1,4-glucan lyase in the fungus Anthracobia melaloma.","publicationDate":"2004","journal":"Biochim. Biophys. Acta","firstPage":"120","lastPage":"129","volume":"1672"},"referencePositions":["PARTIAL PROTEIN SEQUENCE","FUNCTION","CATALYTIC ACTIVITY"]},{"referenceNumber":2,"citation":{"id":"16302767","citationType":"journal article","authors":["Yuan Y.","Mo S.","Cao R.","Westh B.C.","Yu S."],"citationCrossReferences":[{"database":"PubMed","id":"16302767"},{"database":"DOI","id":"10.1021/jf0515274"}],"title":"Examination of 1,5-anhydro-D-fructose and the enolone ascopyrone P, metabolites of the anhydrofructose pathway of glycogen and starch degradation, for their possible application in fruits, vegetables, and beverages as antibrowning agents.","publicationDate":"2005","journal":"J. Agric. Food Chem.","firstPage":"9491","lastPage":"9497","volume":"53"},"referencePositions":["BIOTECHNOLOGY"]}],"uniProtKBCrossReferences":[{"database":"GO","id":"GO:0034018","properties":[{"key":"GoTerm","value":"F:ascopyrone tautomerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]}],"sequence":{"value":"AINLPFSNWAXCTIICYEDEHVSGAVRETEFNTIASILQIGAEHGGTHNFPIIDSECSMPISLEPHLPGITSYLTALSTRNLXXFITEIYGMDARYDPEAAPGSGNP","length":107,"molWeight":11618,"crc64":"60286B01F35D922D","md5":"327E2F3E01CB0EC6D5A2D5A9C578633C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"BIOTECHNOLOGY":1},"countByFeatureType":{"Chain":1,"Non-adjacent residues":7,"Non-terminal residue":2},"uniParcId":"UPI00496DC2E9"}}]}