{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"I6UC43","uniProtkbId":"I6UC43_9CICH","entryAudit":{"firstPublicDate":"2012-10-03","lastAnnotationUpdateDate":"2025-02-05","lastSequenceUpdateDate":"2012-10-03","entryVersion":54,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Astatotilapia flaviijosephi","taxonId":205334,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFM92667.1"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Actinopterygii","Neopterygii","Teleostei","Neoteleostei","Acanthomorphata","Ovalentaria","Cichlomorphae","Cichliformes","Cichlidae","African cichlids","Pseudocrenilabrinae","Haplochromini","Astatotilapia"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00021008"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"value":"NADH-ubiquinone oxidoreductase chain 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012944"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"value":"7.1.1.2"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFM92667.1"}],"value":"ND2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007012"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"value":"Belongs to the complex I subunit 2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}]},{"type":"Transmembrane","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}]},{"type":"Transmembrane","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":218,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}]},{"type":"Transmembrane","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}]},{"type":"Transmembrane","location":{"start":{"value":273,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}]},{"type":"Domain","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Domain","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":319,"modifier":"EXACT"}},"description":"NADH dehydrogenase subunit 2 C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF06444"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFM92667.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":319,"modifier":"EXACT"},"end":{"value":319,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFM92667.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFM92667.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003403"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"22722840","citationType":"journal article","authors":["Wagner C.E.","Harmon L.J.","Seehausen O."],"citationCrossReferences":[{"database":"PubMed","id":"22722840"},{"database":"DOI","id":"10.1038/nature11144"}],"title":"Ecological opportunity and sexual selection together predict adaptive radiation.","publicationDate":"2012","journal":"Nature","firstPage":"366","lastPage":"369","volume":"0"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFM92667.1"}]},{"referenceNumber":2,"citation":{"id":"CI-6DV1CRBFCVKQ0","citationType":"submission","authors":["Mwaiko S.","Bezault E.","Seehausen O."],"title":"Inferring the evolutionary history of the fastest and largest known animal adaptive radiation.","publicationDate":"APR-2012","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AFM92667.1"}]},{"referenceNumber":3,"citation":{"id":"CI-4VVVROQUA7BKL","citationType":"submission","authors":["Song W.-J.","Kurnit D.M."],"publicationDate":"DEC-2016","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQR57239.1"}],"value":"14","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQR57237.1"}],"value":"3","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQR57238.1"}],"value":"8","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQR57237.1"}]},{"referenceNumber":4,"citation":{"id":"28186104","citationType":"journal article","authors":["Meier J.I.","Marques D.A.","Mwaiko S.","Wagner C.E.","Excoffier L.","Seehausen O."],"citationCrossReferences":[{"database":"PubMed","id":"28186104"}],"title":"Ancient hybridization fuels rapid cichlid fish adaptive radiations.","publicationDate":"2017","journal":"Nat. Commun.","firstPage":"14363","lastPage":"14363","volume":"8"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQR57239.1"}],"value":"14","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQR57237.1"}],"value":"3","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQR57238.1"}],"value":"8","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AQR57237.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"JQ950380","properties":[{"key":"ProteinId","value":"AFM92667.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KY366719","properties":[{"key":"ProteinId","value":"AQR57237.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KY366720","properties":[{"key":"ProteinId","value":"AQR57238.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KY366721","properties":[{"key":"ProteinId","value":"AQR57239.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006120","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, NADH to ubiquinone"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"InterPro","id":"IPR050175","properties":[{"key":"EntryName","value":"Complex_I_Subunit_2"}]},{"database":"InterPro","id":"IPR010933","properties":[{"key":"EntryName","value":"NADH_DH_su2_C"}]},{"database":"InterPro","id":"IPR003917","properties":[{"key":"EntryName","value":"NADH_UbQ_OxRdtase_chain2"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"PANTHER","id":"PTHR46552","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46552:SF1","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06444","properties":[{"key":"EntryName","value":"NADH_dehy_S2_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01436","properties":[{"key":"EntryName","value":"NADHDHGNASE2"}]}],"sequence":{"value":"MNPYILATLLFGLGLGTTITFASSHWLLAWMGLEMNTLAIIPLMAQHHHPRAVEATTKYFLTQATAAATLLFASVTNAWLTGQWEIQQITHPLPGTMITLALALKIGLAPLHAWLPEVLQGLDLTTGLILSTWQKLAPFALILQLQPSNSTLLITLGLTSTLNGGWGGLNPNQLRKILAYSSIAHLGWMILVXQFSPSITLLTLLTYFIMTSSMFLVFKHNKSTNINTLATSWTKAPALTALTPLILLSLGGLPPLTGFMPKWLILQELTKQGLAPTATLAALSALLSLYFYLRLSYAMTLTISPNNLTGLTPWRLPST","length":319,"molWeight":34749,"crc64":"C612691C45FCD6E5","md5":"EA02B800E8DD2B9309F50394C2ADEAAB"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5,"Domain":2,"Non-terminal residue":2},"uniParcId":"UPI00026B6D3B"}}]}