{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q8M1L2","uniProtkbId":"Q8M1L2_9PASS","entryAudit":{"firstPublicDate":"2002-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-10-01","entryVersion":99,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Nesospingus speculiferus","taxonId":200198,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM77778.1"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Archelosauria","Archosauria","Dinosauria","Saurischia","Theropoda","Coelurosauria","Aves","Neognathae","Neoaves","Telluraves","Australaves","Passeriformes","Thraupidae","Nesospingus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013531"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"value":"Cytochrome b"}},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM77778.1"}],"value":"cytb"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00002566"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"value":"Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"heme b","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:60344"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"value":"Binds 2 heme groups non-covalently."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"heme","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-2"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:30413"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-2"}],"value":"Binds 2 heme groups non-covalently."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004448"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00061233"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"value":"Belongs to the cytochrome b family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Transmembrane","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Transmembrane","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Transmembrane","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Transmembrane","location":{"start":{"value":197,"modifier":"EXACT"},"end":{"value":218,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Transmembrane","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":276,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Transmembrane","location":{"start":{"value":288,"modifier":"EXACT"},"end":{"value":308,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Transmembrane","location":{"start":{"value":315,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"Cytochrome b/b6 N-terminal region profile","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51002"}]},{"type":"Domain","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"Cytochrome b/b6 C-terminal region profile","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51003"}]},{"type":"Binding site","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60344"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-2"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"ligand":{"name":"heme b","id":"ChEBI:CHEBI:60344","label":"b562"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}},{"type":"Binding site","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60344"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-2"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"ligand":{"name":"heme b","id":"ChEBI:CHEBI:60344","label":"b566"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}},{"type":"Binding site","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60344"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-2"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"ligand":{"name":"heme b","id":"ChEBI:CHEBI:60344","label":"b562"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}},{"type":"Binding site","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:60344"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-2"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"ligand":{"name":"heme b","id":"ChEBI:CHEBI:60344","label":"b566"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}},{"type":"Binding site","location":{"start":{"value":169,"modifier":"EXACT"},"end":{"value":169,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:16389"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-1"}],"ligand":{"name":"a ubiquinone","id":"ChEBI:CHEBI:16389"}},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM77778.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":347,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM77778.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM77778.1"}],"geneEncodingType":"Mitochondrion"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022982"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022617"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-2"}],"id":"KW-0349","category":"Ligand","name":"Heme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-2"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR038885-2"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022792"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022660"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362117"}],"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00968"}],"id":"KW-0813","category":"Biological process","name":"Transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023075"}],"id":"KW-0830","category":"Ligand","name":"Ubiquinone"}],"references":[{"referenceNumber":1,"citation":{"id":"12144023","citationType":"journal article","authors":["Burns K.J.","Hackett S.J.","Klein N.K."],"citationCrossReferences":[{"database":"PubMed","id":"12144023"}],"title":"Phylogenetic relationships and morphological diversity in Darwin's finches and their relatives.","publicationDate":"2002","journal":"Evolution","firstPage":"1240","lastPage":"1252","volume":"56"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAM77778.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF489889","properties":[{"key":"ProteinId","value":"AAM77778.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q8M1L2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0045275","properties":[{"key":"GoTerm","value":"C:respiratory chain complex III"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008121","properties":[{"key":"GoTerm","value":"F:quinol-cytochrome-c reductase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006122","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, ubiquinol to cytochrome c"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd00290","properties":[{"key":"EntryName","value":"cytochrome_b_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00284","properties":[{"key":"EntryName","value":"Cytochrome_b_N"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.810.10:FF:000002","properties":[{"key":"EntryName","value":"Cytochrome b"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.810.10","properties":[{"key":"EntryName","value":"Cytochrome Bc1 Complex, Chain C"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005798","properties":[{"key":"EntryName","value":"Cyt_b/b6_C"}]},{"database":"InterPro","id":"IPR036150","properties":[{"key":"EntryName","value":"Cyt_b/b6_C_sf"}]},{"database":"InterPro","id":"IPR005797","properties":[{"key":"EntryName","value":"Cyt_b/b6_N"}]},{"database":"InterPro","id":"IPR027387","properties":[{"key":"EntryName","value":"Cytb/b6-like_sf"}]},{"database":"InterPro","id":"IPR030689","properties":[{"key":"EntryName","value":"Cytochrome_b"}]},{"database":"InterPro","id":"IPR048260","properties":[{"key":"EntryName","value":"Cytochrome_b_C_euk/bac"}]},{"database":"InterPro","id":"IPR048259","properties":[{"key":"EntryName","value":"Cytochrome_b_N_euk/bac"}]},{"database":"InterPro","id":"IPR016174","properties":[{"key":"EntryName","value":"Di-haem_cyt_TM"}]},{"database":"PANTHER","id":"PTHR19271","properties":[{"key":"EntryName","value":"CYTOCHROME B"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR19271:SF16","properties":[{"key":"EntryName","value":"CYTOCHROME B"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00032","properties":[{"key":"EntryName","value":"Cytochrom_B_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00033","properties":[{"key":"EntryName","value":"Cytochrome_B"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF038885","properties":[{"key":"EntryName","value":"COB"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81648","properties":[{"key":"EntryName","value":"a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81342","properties":[{"key":"EntryName","value":"Transmembrane di-heme cytochromes"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51003","properties":[{"key":"EntryName","value":"CYTB_CTER"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51002","properties":[{"key":"EntryName","value":"CYTB_NTER"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"FGSLLGLCLITQIVTGLLLAMHYTADTSLAFSSVAHMCRDVQFGWLIRNLHANGASFFFICVYLHIGRGLYYGSYLYKETWNVGVILLLILMATAFVGYVLPWGQMSFWGATVITNLFSAIPYIGQTLVEWAWGGFSVDNPTLTRFFALHFLLPFVIVGITLVHLTFLHETGSNNPLGIPSDCDKIPFHPYYTIKDILGFVLMLSLLVSLALFAPNLLGDPENFTPANPLVTPPHIKPEWYFLFAYAILRSIPNKLGGVLALAASILVLFLTPLLHTSKLRSMTFRPLSQILFWTLVANILILTWVGSQPVEHPFIIIGQLASFTYFTIILVLFPLAALLENKLLKL","length":347,"molWeight":38713,"crc64":"E95CE1E7C0938ECA","md5":"335131515ECC00082297D60A30A320EC"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":2,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":8,"Domain":2,"Binding site":5,"Non-terminal residue":2},"uniParcId":"UPI00000966A4"}}]}