{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q49CC7","uniProtkbId":"Q49CC7_9ASTE","entryAudit":{"firstPublicDate":"2005-09-13","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-09-13","entryVersion":97,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Evolvulus glomeratus","taxonId":197388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58006.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","asterids","lamiids","Solanales","Convolvulaceae","Cresseae","Evolvulus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"value":"NAD(P)H-quinone oxidoreductase subunit J, chloroplastic"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"value":"NAD(P)H dehydrogenase, subunit J"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"value":"NADH-plastoquinone oxidoreductase subunit J"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"value":"ndhJ"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003581"}],"value":"NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42608"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003581"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42612"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003581"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003581"}],"value":"NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004443"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004443"}],"value":"Peripheral membrane protein","id":"SL-9903"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004443"}],"value":"Matrix side","id":"SL-9913"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003581"}],"value":"Plastid, chloroplast thylakoid membrane","id":"SL-0058"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003581"}],"value":"Peripheral membrane protein","id":"SL-9903"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003581"}],"value":"Stromal side","id":"SL-9917"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007569"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003456"}],"value":"Belongs to the complex I 30 kDa subunit family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"NADH:ubiquinone oxidoreductase 30kDa subunit","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00329"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58006.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003581"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022640"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003581"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022957"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"id":"KW-0618","category":"Ligand","name":"Plastoquinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022719"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"id":"KW-0874","category":"PTM","name":"Quinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023078"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"id":"KW-0793","category":"Cellular component","name":"Thylakoid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01357"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"15999247","citationType":"journal article","authors":["Stefanovic S.","Olmstead R.G."],"citationCrossReferences":[{"database":"PubMed","id":"15999247"},{"database":"DOI","id":"10.1007/s00239-004-0267-5"}],"title":"Down the slippery slope: plastid genome evolution in Convolvulaceae.","publicationDate":"2005","journal":"J. Mol. Evol.","firstPage":"292","lastPage":"305","volume":"61"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58006.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY936342","properties":[{"key":"ProteinId","value":"AAY58006.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q49CC7","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009535","properties":[{"key":"GoTerm","value":"C:chloroplast thylakoid membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0048038","properties":[{"key":"GoTerm","value":"F:quinone binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019684","properties":[{"key":"GoTerm","value":"P:photosynthesis, light reaction"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"FunFam","id":"3.30.460.80:FF:000004","properties":[{"key":"EntryName","value":"NAD(P)H-quinone oxidoreductase subunit J, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.460.80","properties":[{"key":"EntryName","value":"NADH:ubiquinone oxidoreductase, 30kDa subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01357","properties":[{"key":"EntryName","value":"NDH1_NuoC"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR010218","properties":[{"key":"EntryName","value":"NADH_DH_suC"}]},{"database":"InterPro","id":"IPR037232","properties":[{"key":"EntryName","value":"NADH_quin_OxRdtase_su_C/D-like"}]},{"database":"InterPro","id":"IPR001268","properties":[{"key":"EntryName","value":"NADH_UbQ_OxRdtase_30kDa_su"}]},{"database":"InterPro","id":"IPR020396","properties":[{"key":"EntryName","value":"NADH_UbQ_OxRdtase_CS"}]},{"database":"NCBIfam","id":"NF009141","properties":[{"key":"EntryName","value":"PRK12494.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10884:SF14","properties":[{"key":"EntryName","value":"NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10884","properties":[{"key":"EntryName","value":"NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00329","properties":[{"key":"EntryName","value":"Complex1_30kDa"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF143243","properties":[{"key":"EntryName","value":"Nqo5-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00542","properties":[{"key":"EntryName","value":"COMPLEX1_30K"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MQSYLSVWLVKHGIIHRSLGFDYQGIETLQIKPEDWHSVAVILYVYGYNYLRSQCAYDVAPGGLLASVYHLTRIEYGVTRIEYGAYQPEEVCIKVFASRKNPRIPSVFWIWKSADFQERESYDMLGISYDNHPRLKRILMPESWIGWPLRKDYVAPHFYEIQDAY","length":165,"molWeight":19492,"crc64":"31C982F0E705319F","md5":"2F223166B1DF80323F52935152192EF3"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":3,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1},"uniParcId":"UPI00005859CE"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q49CC8","uniProtkbId":"Q49CC8_9ASTE","entryAudit":{"firstPublicDate":"2005-09-13","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2005-09-13","entryVersion":81,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Evolvulus glomeratus","taxonId":197388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58005.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","asterids","lamiids","Solanales","Convolvulaceae","Cresseae","Evolvulus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"NAD(P)H-quinone oxidoreductase subunit K, chloroplastic"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"NAD(P)H dehydrogenase subunit K"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"NADH-plastoquinone oxidoreductase subunit K"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"ndhK"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42608"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048026"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42612"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047726"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"[4Fe-4S] cluster","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49883"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"Binds 1 [4Fe-4S] cluster."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011199"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004185"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"Plastid, chloroplast thylakoid membrane","id":"SL-0058"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004185"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"Peripheral membrane protein","id":"SL-9903"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004185"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"value":"Stromal side","id":"SL-9917"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009173"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU004464"}],"value":"Belongs to the complex I 20 kDa subunit family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"NADH:ubiquinone oxidoreductase-like 20kDa subunit","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01058"}]},{"type":"Binding site","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}},{"type":"Binding site","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:49883"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"ligand":{"name":"[4Fe-4S] cluster","id":"ChEBI:CHEBI:49883"}}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58005.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022485"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0004","category":"Ligand","name":"4Fe-4S"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58005.1"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023014"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58005.1"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022957"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0618","category":"Ligand","name":"Plastoquinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022719"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0874","category":"PTM","name":"Quinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023078"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0793","category":"Cellular component","name":"Thylakoid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01356"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"15999247","citationType":"journal article","authors":["Stefanovic S.","Olmstead R.G."],"citationCrossReferences":[{"database":"PubMed","id":"15999247"},{"database":"DOI","id":"10.1007/s00239-004-0267-5"}],"title":"Down the slippery slope: plastid genome evolution in Convolvulaceae.","publicationDate":"2005","journal":"J. Mol. Evol.","firstPage":"292","lastPage":"305","volume":"61"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58005.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY936342","properties":[{"key":"ProteinId","value":"AAY58005.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q49CC8","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009535","properties":[{"key":"GoTerm","value":"C:chloroplast thylakoid membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0045271","properties":[{"key":"GoTerm","value":"C:respiratory chain complex I"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0051539","properties":[{"key":"GoTerm","value":"F:4 iron, 4 sulfur cluster binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0048038","properties":[{"key":"GoTerm","value":"F:quinone binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0009060","properties":[{"key":"GoTerm","value":"P:aerobic respiration"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0015990","properties":[{"key":"GoTerm","value":"P:electron transport coupled proton transport"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0019684","properties":[{"key":"GoTerm","value":"P:photosynthesis, light reaction"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"FunFam","id":"3.40.50.12280:FF:000003","properties":[{"key":"EntryName","value":"NAD(P)H-quinone oxidoreductase subunit K, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.12280","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01356","properties":[{"key":"EntryName","value":"NDH1_NuoB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006137","properties":[{"key":"EntryName","value":"NADH_UbQ_OxRdtase-like_20kDa"}]},{"database":"InterPro","id":"IPR006138","properties":[{"key":"EntryName","value":"NADH_UQ_OxRdtase_20Kd_su"}]},{"database":"NCBIfam","id":"NF005012","properties":[{"key":"EntryName","value":"nuoB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01957","properties":[{"key":"EntryName","value":"nuoB_fam"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11995","properties":[{"key":"EntryName","value":"NADH DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11995:SF14","properties":[{"key":"EntryName","value":"NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01058","properties":[{"key":"EntryName","value":"Oxidored_q6"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56770","properties":[{"key":"EntryName","value":"HydA/Nqo6-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01150","properties":[{"key":"EntryName","value":"COMPLEX1_20K"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNSIQFPLLDRTAKNSVISTTNSVISTTLNDLSNWSRLSSLWPLLYGTSCCFIEFASLIGSRFDFDRYGLVPRSSPRQADLILTAGTVTMKMAPSLVRLYEQMPEPKYVIAMGACTITGGMFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRMKSQQANRCFTTNHKFCVGHSSHFENYDQRFFSQPSEIPTETEMFSHTKIQYLPTT","length":228,"molWeight":25740,"crc64":"F84B94972EAC6BCB","md5":"A138703273D12DDF6843CE2F2C34B36B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Binding site":4},"uniParcId":"UPI00005859CD"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q49CC9","uniProtkbId":"Q49CC9_9ASTE","entryAudit":{"firstPublicDate":"2005-09-13","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-09-13","entryVersion":67,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Evolvulus glomeratus","taxonId":197388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58004.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","asterids","lamiids","Solanales","Convolvulaceae","Cresseae","Evolvulus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"value":"NAD(P)H-quinone oxidoreductase subunit 3, chloroplastic"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"value":"NAD(P)H dehydrogenase subunit 3"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"value":"NADH-plastoquinone oxidoreductase subunit 3"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"value":"ndhC"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003257"}],"value":"Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"value":"NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42608"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003641"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42612"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003641"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29091"},{"database":"Rhea","id":"RHEA-COMP:9565"},{"database":"Rhea","id":"RHEA-COMP:9566"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16389"},{"database":"ChEBI","id":"CHEBI:17976"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"}],"ecNumber":"7.1.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049551"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"value":"NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004225"}],"value":"Mitochondrion membrane","id":"SL-0171"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004225"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"value":"Plastid, chloroplast thylakoid membrane","id":"SL-0058"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008472"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003641"}],"value":"Belongs to the complex I subunit 3 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}]},{"type":"Transmembrane","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}]},{"type":"Transmembrane","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58004.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58004.1"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58004.1"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022957"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-0618","category":"Ligand","name":"Plastoquinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022719"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-0874","category":"PTM","name":"Quinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023078"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-0793","category":"Cellular component","name":"Thylakoid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01394"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"15999247","citationType":"journal article","authors":["Stefanovic S.","Olmstead R.G."],"citationCrossReferences":[{"database":"PubMed","id":"15999247"},{"database":"DOI","id":"10.1007/s00239-004-0267-5"}],"title":"Down the slippery slope: plastid genome evolution in Convolvulaceae.","publicationDate":"2005","journal":"J. Mol. Evol.","firstPage":"292","lastPage":"305","volume":"61"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58004.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY936342","properties":[{"key":"ProteinId","value":"AAY58004.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q49CC9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009535","properties":[{"key":"GoTerm","value":"C:chloroplast thylakoid membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0031966","properties":[{"key":"GoTerm","value":"C:mitochondrial membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0030964","properties":[{"key":"GoTerm","value":"C:NADH dehydrogenase complex"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0019684","properties":[{"key":"GoTerm","value":"P:photosynthesis, light reaction"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"FunFam","id":"1.20.58.1610:FF:000001","properties":[{"key":"EntryName","value":"NAD(P)H-quinone oxidoreductase subunit 3, chloroplastic"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.58.1610","properties":[{"key":"EntryName","value":"NADH:ubiquinone/plastoquinone oxidoreductase, chain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01394","properties":[{"key":"EntryName","value":"NDH1_NuoA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR023043","properties":[{"key":"EntryName","value":"NAD(P)H_OxRDtase_bac/plastid"}]},{"database":"InterPro","id":"IPR000440","properties":[{"key":"EntryName","value":"NADH_UbQ/plastoQ_OxRdtase_su3"}]},{"database":"InterPro","id":"IPR038430","properties":[{"key":"EntryName","value":"NDAH_ubi_oxred_su3_sf"}]},{"database":"PANTHER","id":"PTHR11058","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11058:SF9","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00507","properties":[{"key":"EntryName","value":"Oxidored_q4"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFLLYEYDIFWAFLIISSLIPILTFLISGVLAPISKGPEKLSSYESGIEPMGDAWLQFRIRYYMFALVFVVFDVETVFLYPWAMSFDVLGVSVFLEAFIFVLILIGGLVYAWRKGALEWS","length":120,"molWeight":13830,"crc64":"E0FBB6997FAEB51C","md5":"9194A8340E970658258B873ED22D2F31"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":3,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":3},"uniParcId":"UPI00005859CC"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q49CD0","uniProtkbId":"Q49CD0_9ASTE","entryAudit":{"firstPublicDate":"2005-09-13","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2005-09-13","entryVersion":90,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Evolvulus glomeratus","taxonId":197388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58003.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","asterids","lamiids","Solanales","Convolvulaceae","Cresseae","Evolvulus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00018648"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NADH-plastoquinone oxidoreductase subunit 5"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"ndhF"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004059"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42608"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048026"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42612"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047726"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011199"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004454"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Plastid, chloroplast thylakoid membrane","id":"SL-0058"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004454"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008200"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Belongs to the complex I subunit 5 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":231,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":271,"modifier":"EXACT"},"end":{"value":291,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":297,"modifier":"EXACT"},"end":{"value":322,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":350,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":404,"modifier":"EXACT"},"end":{"value":423,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":435,"modifier":"EXACT"},"end":{"value":456,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":527,"modifier":"EXACT"},"end":{"value":546,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":583,"modifier":"EXACT"},"end":{"value":601,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Domain","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":136,"modifier":"EXACT"}},"description":"NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00662"}]},{"type":"Domain","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":452,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Domain","location":{"start":{"value":459,"modifier":"EXACT"},"end":{"value":511,"modifier":"EXACT"}},"description":"NADH:ubiquinone/plastoquinone oxidoreductase chloroplast chain 5 C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01010"}]},{"type":"Domain","location":{"start":{"value":517,"modifier":"EXACT"},"end":{"value":667,"modifier":"EXACT"}},"description":"NADH:ubiquinone/plastoquinone oxidoreductase chloroplast chain 5 C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01010"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58003.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":694,"modifier":"EXACT"},"end":{"value":694,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58003.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58003.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022640"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022957"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0618","category":"Ligand","name":"Plastoquinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022719"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0874","category":"PTM","name":"Quinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023078"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0793","category":"Cellular component","name":"Thylakoid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"15999247","citationType":"journal article","authors":["Stefanovic S.","Olmstead R.G."],"citationCrossReferences":[{"database":"PubMed","id":"15999247"},{"database":"DOI","id":"10.1007/s00239-004-0267-5"}],"title":"Down the slippery slope: plastid genome evolution in Convolvulaceae.","publicationDate":"2005","journal":"J. Mol. Evol.","firstPage":"292","lastPage":"305","volume":"61"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAY58003.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY936341","properties":[{"key":"ProteinId","value":"AAY58003.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"GO","id":"GO:0009535","properties":[{"key":"GoTerm","value":"C:chloroplast thylakoid membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0042773","properties":[{"key":"GoTerm","value":"P:ATP synthesis coupled electron transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0015990","properties":[{"key":"GoTerm","value":"P:electron transport coupled proton transport"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR002128","properties":[{"key":"EntryName","value":"NADH_UbQ_OxRdtase_chlpt_su5_C"}]},{"database":"InterPro","id":"IPR018393","properties":[{"key":"EntryName","value":"NADHpl_OxRdtase_5_subgr"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"InterPro","id":"IPR003945","properties":[{"key":"EntryName","value":"NU5C-like"}]},{"database":"InterPro","id":"IPR001516","properties":[{"key":"EntryName","value":"Proton_antipo_N"}]},{"database":"NCBIfam","id":"TIGR01974","properties":[{"key":"EntryName","value":"NDH_I_L"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF005141","properties":[{"key":"EntryName","value":"nuoL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42829","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42829:SF2","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01010","properties":[{"key":"EntryName","value":"Proton_antipo_C"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00662","properties":[{"key":"EntryName","value":"Proton_antipo_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01434","properties":[{"key":"EntryName","value":"NADHDHGNASE5"}]},{"database":"PRINTS","id":"PR01435","properties":[{"key":"EntryName","value":"NPOXDRDTASE5"}]}],"sequence":{"value":"ETLIKTKMKMFMEQTYQYAWILPFVPLPVPILIGAGLLLFPIVTKNIRRMWSFQSILLLSIVMAFSIYLSIEQINNGSIYQYVWSWILNNDFSLEFGYYLDPLTSIMSILITTVGIMVLIYSDSYMAHDRGYLRFFAYMSFFSVTMLGLVTSSNLIQIYIFWELVGLCSYLLIGFWFTRPGVANACQKAFVTNRVGDFGLLLGILGCYWITGSFEFRELFEIVNNLISSNQVNFLFLTLCTALLFVGAIAKSAQFPLHIWLPDAMEGPTPISALIHAATMVAAGIFLVARLLPLFVIIPYIMSFISLIGIITVLLGATLAIAQQDIKRCLAYSTMSQLGYMMLALGMGSYRNALFHLITHAYSKALLFLGSGSVIHSMETIVGYSPDKSQNMVLMGGLTKYVPITKGAFLLGTLSLCGIPPLACFWSKDEILHDSWLYSPVVALIAWATAGLTAFYXXRIYLLTFEGHLNVRLQHYSAKKDTSPDSISLWGKKASKEINRNFPVLQMAXNQPQISYPSYPXESDNTMLFPICILCLFTLLVGFLGIPXEIGFNLDILAQWLHPPIYLLHETNSRDLLDFLKNAATSVGIAYYGILIACLFYKPTYSSFNTFHLINSFHQKSPKRVLRDKIVTVIYDWSAIRAYIDAFYKKFIVYQVRRLXHFSYFFDFRITDTKVNGVGFFSFLVGEGIKYXGG","length":694,"molWeight":78443,"crc64":"91180CFA5244BFB6","md5":"0CC5C4E6547DF3EC4BB4E7F48797CE91"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Transmembrane":13,"Domain":4,"Non-terminal residue":2},"uniParcId":"UPI00005859CB"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A9YEW1","uniProtkbId":"A9YEW1_9ASTE","entryAudit":{"firstPublicDate":"2008-02-05","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2008-02-05","entryVersion":42,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Evolvulus glomeratus","taxonId":197388,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABY25290.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","asterids","lamiids","Solanales","Convolvulaceae","Cresseae","Evolvulus"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00039963"}],"value":"Dihydroflavonol 4-reductase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00039057"}],"value":"1.1.1.219"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00039055"}],"value":"1.1.1.234"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00042831"}],"value":"Dihydrokaempferol 4-reductase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00042087"}],"value":"Flavanone 4-reductase"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00037100"}],"value":"Bifunctional enzyme involved in flavonoid metabolism"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11228"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15605"},{"database":"ChEBI","id":"CHEBI:15606"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"ecNumber":"1.1.1.234","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048870"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-dihydroflavonol + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:54444"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:138176"},{"database":"ChEBI","id":"CHEBI:138188"}],"ecNumber":"1.1.1.219","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049132"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004935"}],"value":"Pigment biosynthesis; anthocyanin biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023445"}],"value":"Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":254,"modifier":"EXACT"}},"description":"NAD-dependent epimerase/dehydratase","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01370"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023241"}],"id":"KW-0284","category":"Biological process","name":"Flavonoid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"18594508","citationType":"journal article","authors":["Des Marais D.L.","Rausher M.D."],"citationCrossReferences":[{"database":"PubMed","id":"18594508"},{"database":"DOI","id":"10.1038/nature07092"}],"title":"Escape from adaptive conflict after duplication in an anthocyanin pathway gene.","publicationDate":"2008","journal":"Nature","firstPage":"762","lastPage":"765","volume":"454"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABY25290.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"EU189082","properties":[{"key":"ProteinId","value":"ABY25290.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"A9YEW1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0045552","properties":[{"key":"GoTerm","value":"F:dihydroflavanol 4-reductase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0047890","properties":[{"key":"GoTerm","value":"F:flavanone 4-reductase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0009718","properties":[{"key":"GoTerm","value":"P:anthocyanin-containing compound biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd08958","properties":[{"key":"EntryName","value":"FR_SDR_e"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.720:FF:000085","properties":[{"key":"EntryName","value":"Dihydroflavonol reductase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001509","properties":[{"key":"EntryName","value":"Epimerase_deHydtase"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"InterPro","id":"IPR050425","properties":[{"key":"EntryName","value":"NAD(P)_dehydrat-like"}]},{"database":"PANTHER","id":"PTHR10366","properties":[{"key":"EntryName","value":"NAD DEPENDENT EPIMERASE/DEHYDRATASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10366:SF564","properties":[{"key":"EntryName","value":"STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01370","properties":[{"key":"EntryName","value":"Epimerase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVGNDDQHNVVSKVCVTGATGFIGSWLVMRLLERDYIVHATVRDPGNMEKVKHLLELPKADKNLRLWRGVLEEEGSFDEAIEGCEGVFHVATPMDFDSEDPENEVIKPTVKGILGIIDSCAKSKSVKRIVFTSSAGTVDIQENQKSLYDETCWSDLDFIYAKKMTGWMYFASKILAEKEAWKSTKEKQIDFISIIPPVVVGPFITPTFPPSLITALSPIMGNESHCRHIKQGQFVHIDDLCEALMFLYEHPKAQGRFICSSHHTTIHDIAKMIRHNWPEYNVPNEFKGIEKDIEVVSFSSKKLLDMGFQFKYTLEDMYRGAIETSRNKGLLPYSIKDPAAYALKIRTIDN","length":350,"molWeight":39669,"crc64":"B4C0E6EE144AE8DA","md5":"BEFC1D4746081BB7526DD71B84527C18"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"PATHWAY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1},"uniParcId":"UPI000162E566"}}]}