{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NRF0","uniProtkbId":"A0A1B7NRF0_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":43,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79365.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00081752"}],"value":"Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013008"}],"value":"2.1.3.2"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012918"}],"value":"3.5.1.2"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00044063"}],"value":"6.3.4.16"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012738"}],"value":"6.3.5.5"}]}},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79365.1"}],"value":"ACJ72_06317"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00058513"}],"value":"Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-glutamine + H2O = L-glutamate + NH4(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15889"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:29985"},{"database":"ChEBI","id":"CHEBI:58359"}],"ecNumber":"3.5.1.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049534"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20013"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29991"},{"database":"ChEBI","id":"CHEBI:32814"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58228"}],"ecNumber":"2.1.3.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048859"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18633"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17544"},{"database":"ChEBI","id":"CHEBI:29985"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58228"},{"database":"ChEBI","id":"CHEBI:58359"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"6.3.5.5","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048816"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18029"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17544"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58228"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"6.3.4.16","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047359"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001947"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004812"}],"value":"Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004852"}],"value":"Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00043998"}],"value":"In the 2nd section; belongs to the CarB family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00043968"}],"value":"In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00043979"}],"value":"In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00043984"}],"value":"In the N-terminal section; belongs to the CarA family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79365.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":630,"modifier":"EXACT"},"end":{"value":822,"modifier":"EXACT"}},"description":"ATP-grasp","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50975"}]},{"type":"Domain","location":{"start":{"value":1165,"modifier":"EXACT"},"end":{"value":1356,"modifier":"EXACT"}},"description":"ATP-grasp","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50975"}]},{"type":"Domain","location":{"start":{"value":1422,"modifier":"EXACT"},"end":{"value":1601,"modifier":"EXACT"}},"description":"MGS-like","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51855"}]},{"type":"Active site","location":{"start":{"value":368,"modifier":"EXACT"},"end":{"value":368,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00605"}]},{"type":"Active site","location":{"start":{"value":452,"modifier":"EXACT"},"end":{"value":452,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00605"}]},{"type":"Active site","location":{"start":{"value":454,"modifier":"EXACT"},"end":{"value":454,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00605"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00409"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00605"}],"id":"KW-0315","category":"Domain","name":"Glutamine amidotransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022598"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00409"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022553"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022975"}],"id":"KW-0665","category":"Biological process","name":"Pyrimidine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022737"}],"id":"KW-0677","category":"Domain","name":"Repeat"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79365.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79365.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01001050","properties":[{"key":"ProteinId","value":"OAX79365.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"STRING","id":"1658172.A0A1B7NRF0","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1924069at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00070","properties":[{"key":"RectionId","value":"UER00115"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0016597","properties":[{"key":"GoTerm","value":"F:amino acid binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004070","properties":[{"key":"GoTerm","value":"F:aspartate carbamoyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004087","properties":[{"key":"GoTerm","value":"F:carbamoyl-phosphate synthase (ammonia) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004088","properties":[{"key":"GoTerm","value":"F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004151","properties":[{"key":"GoTerm","value":"F:dihydroorotase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004359","properties":[{"key":"GoTerm","value":"F:glutaminase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006207","properties":[{"key":"GoTerm","value":"P:'de novo' pyrimidine nucleobase biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0044205","properties":[{"key":"GoTerm","value":"P:'de novo' UMP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"GO","id":"GO:0019240","properties":[{"key":"GoTerm","value":"P:L-citrulline biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006541","properties":[{"key":"GoTerm","value":"P:L-glutamine metabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006228","properties":[{"key":"GoTerm","value":"P:UTP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd01744","properties":[{"key":"EntryName","value":"GATase1_CPSase"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01423","properties":[{"key":"EntryName","value":"MGS_CPS_I_III"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.1370:FF:000002","properties":[{"key":"EntryName","value":"Aspartate carbamoyltransferase 2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.880:FF:000025","properties":[{"key":"EntryName","value":"Bifunctional pyrimidine biosynthesis protein"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.1370:FF:000005","properties":[{"key":"EntryName","value":"CAD protein-like isoform X1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.20:FF:000011","properties":[{"key":"EntryName","value":"CAD protein-like isoform X1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.470.20:FF:000004","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.50.30.20:FF:000002","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase 1, mitochondrial"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.1030.10:FF:000001","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase large chain"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.140:FF:000036","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase large chain"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.470.20:FF:000001","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase large chain"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.20:FF:000002","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase large chain"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.1380:FF:000009","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase, large subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.40.50.880","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1370","properties":[{"key":"EntryName","value":"Aspartate/ornithine carbamoyltransferase"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.30.1490.20","properties":[{"key":"EntryName","value":"ATP-grasp fold, A domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.470.20","properties":[{"key":"EntryName","value":"ATP-grasp fold, B domain"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.50.30.20","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthase small subunit, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.1030.10","properties":[{"key":"EntryName","value":"Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.140","properties":[{"key":"EntryName","value":"Metal-dependent hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1380","properties":[{"key":"EntryName","value":"Methylglyoxal synthase-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00001","properties":[{"key":"EntryName","value":"Asp_carb_tr"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01209","properties":[{"key":"EntryName","value":"CPSase_S_chain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006132","properties":[{"key":"EntryName","value":"Asp/Orn_carbamoyltranf_P-bd"}]},{"database":"InterPro","id":"IPR006130","properties":[{"key":"EntryName","value":"Asp/Orn_carbamoylTrfase"}]},{"database":"InterPro","id":"IPR036901","properties":[{"key":"EntryName","value":"Asp/Orn_carbamoylTrfase_sf"}]},{"database":"InterPro","id":"IPR002082","properties":[{"key":"EntryName","value":"Asp_carbamoyltransf"}]},{"database":"InterPro","id":"IPR006131","properties":[{"key":"EntryName","value":"Asp_carbamoyltransf_Asp/Orn-bd"}]},{"database":"InterPro","id":"IPR011761","properties":[{"key":"EntryName","value":"ATP-grasp"}]},{"database":"InterPro","id":"IPR013815","properties":[{"key":"EntryName","value":"ATP_grasp_subdomain_1"}]},{"database":"InterPro","id":"IPR036897","properties":[{"key":"EntryName","value":"CarbamoylP_synth_lsu_oligo_sf"}]},{"database":"InterPro","id":"IPR006274","properties":[{"key":"EntryName","value":"CarbamoylP_synth_ssu"}]},{"database":"InterPro","id":"IPR002474","properties":[{"key":"EntryName","value":"CarbamoylP_synth_ssu_N"}]},{"database":"InterPro","id":"IPR036480","properties":[{"key":"EntryName","value":"CarbP_synth_ssu_N_sf"}]},{"database":"InterPro","id":"IPR029062","properties":[{"key":"EntryName","value":"Class_I_gatase-like"}]},{"database":"InterPro","id":"IPR005479","properties":[{"key":"EntryName","value":"CPAse_ATP-bd"}]},{"database":"InterPro","id":"IPR005483","properties":[{"key":"EntryName","value":"CPSase_dom"}]},{"database":"InterPro","id":"IPR035686","properties":[{"key":"EntryName","value":"CPSase_GATase1"}]},{"database":"InterPro","id":"IPR006275","properties":[{"key":"EntryName","value":"CPSase_lsu"}]},{"database":"InterPro","id":"IPR005480","properties":[{"key":"EntryName","value":"CPSase_lsu_oligo"}]},{"database":"InterPro","id":"IPR058047","properties":[{"key":"EntryName","value":"CPSase_preATP-grasp"}]},{"database":"InterPro","id":"IPR017926","properties":[{"key":"EntryName","value":"GATASE"}]},{"database":"InterPro","id":"IPR032466","properties":[{"key":"EntryName","value":"Metal_Hydrolase"}]},{"database":"InterPro","id":"IPR011607","properties":[{"key":"EntryName","value":"MGS-like_dom"}]},{"database":"InterPro","id":"IPR036914","properties":[{"key":"EntryName","value":"MGS-like_dom_sf"}]},{"database":"InterPro","id":"IPR016185","properties":[{"key":"EntryName","value":"PreATP-grasp_dom_sf"}]},{"database":"NCBIfam","id":"TIGR00670","properties":[{"key":"EntryName","value":"asp_carb_tr"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF003671","properties":[{"key":"EntryName","value":"carB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01369","properties":[{"key":"EntryName","value":"CPSaseII_lrg"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01368","properties":[{"key":"EntryName","value":"CPSaseIIsmall"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF002032","properties":[{"key":"EntryName","value":"PRK00856.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009455","properties":[{"key":"EntryName","value":"PRK12815.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009475","properties":[{"key":"EntryName","value":"PRK12838.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11405:SF5","properties":[{"key":"EntryName","value":"CAD PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11405","properties":[{"key":"EntryName","value":"CARBAMOYLTRANSFERASE FAMILY MEMBER"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25596","properties":[{"key":"EntryName","value":"CPSase_L_D1"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF02786","properties":[{"key":"EntryName","value":"CPSase_L_D2"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF02787","properties":[{"key":"EntryName","value":"CPSase_L_D3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00988","properties":[{"key":"EntryName","value":"CPSase_sm_chain"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00117","properties":[{"key":"EntryName","value":"GATase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02142","properties":[{"key":"EntryName","value":"MGS"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00185","properties":[{"key":"EntryName","value":"OTCace"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02729","properties":[{"key":"EntryName","value":"OTCace_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00100","properties":[{"key":"EntryName","value":"AOTCASE"}]},{"database":"PRINTS","id":"PR00101","properties":[{"key":"EntryName","value":"ATCASE"}]},{"database":"PRINTS","id":"PR00098","properties":[{"key":"EntryName","value":"CPSASE"}]},{"database":"PRINTS","id":"PR00099","properties":[{"key":"EntryName","value":"CPSGATASE"}]},{"database":"SMART","id":"SM01096","properties":[{"key":"EntryName","value":"CPSase_L_D3"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01097","properties":[{"key":"EntryName","value":"CPSase_sm_chain"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00851","properties":[{"key":"EntryName","value":"MGS"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53671","properties":[{"key":"EntryName","value":"Aspartate/ornithine carbamoyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48108","properties":[{"key":"EntryName","value":"Carbamoyl phosphate synthetase, large subunit connection domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52021","properties":[{"key":"EntryName","value":"Carbamoyl phosphate synthetase, small subunit N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52317","properties":[{"key":"EntryName","value":"Class I glutamine amidotransferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56059","properties":[{"key":"EntryName","value":"Glutathione synthetase ATP-binding domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF51556","properties":[{"key":"EntryName","value":"Metallo-dependent hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52335","properties":[{"key":"EntryName","value":"Methylglyoxal synthase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52440","properties":[{"key":"EntryName","value":"PreATP-grasp domain"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS50975","properties":[{"key":"EntryName","value":"ATP_GRASP"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS00097","properties":[{"key":"EntryName","value":"CARBAMOYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00866","properties":[{"key":"EntryName","value":"CPSASE_1"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS00867","properties":[{"key":"EntryName","value":"CPSASE_2"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51273","properties":[{"key":"EntryName","value":"GATASE_TYPE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51855","properties":[{"key":"EntryName","value":"MGS"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPLLNAAVDAKGHPVLPNSNSTDGVVAYTQNRDLLLSPASQAAYSLNGGAIPPASPPKAGEEGVGSKWYALELEDGTVYQGYSFGADKSTAGELVFQTGMVGYPESITDPSYRGQILVITFPLVGNYGVPSRETVDELLGGDLPKHFESSQIHVAGLVVANYCGEDYSHFLAESSLGKWLKEQGIPAMHGVDTRALTKRIREKGSMLGRMLLQKPDVNVETLNGDAQKDGLTAAAADEEEKSNIWRSFFEQIEWVDPNKRNLVNDVSIRKPRLFSPPKDSALLHPTGRPIRVVCVDVGMKYNQLRCLLSRGVEVLVVPWDYDFPALAGKDYDGLFLSNGPGDPSMLSKTVANITMAIENCRTPIFGICLGHQLLARAAGASTTKMKFGNRGSNIPCTSLISGRCYITSQNHGYAVDSTTLPAGWEELFVNANDGSNEGIRHVGRPIFSVQFHPESVPGPRDTEFLFDVFIDAIKKTVISPNSLTEPVSFPGATAEESQLANPRVSVRKALILGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTVLINPNIATIQTSKGLADKVYFLPVNADFVRKVIKHERPDAIYVTFGGQTALQVGIQLKDEFEELGIRVLGTPIDTIITTEDRELFARSMESIGEKCAKSASASTLEEAMEAVKDIGFPVIVRAAYALGGLGSGFAEDPEQLRDLCVKAFAASPQVLIERSMKGWKEIEYEVVRDARDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFSREYCIIEVNARLSRSSALASKATGYPLAFVAAKLGLGIPLNEISNSVTKVTCACFEPSLDYVVVKIPRWDLKKFTRVSTQLGSSMKSVGEVMSIGRTFEEAIQKAIRSVDFHNIGFNETNALMSIKAELQTPSDQRLFAIANAMHSGYTVEDIWELTKIDKWFLRKLKGLSDFGKLMSGYNATTAPMSLIRQAKQLGFSDRQLSKFLSSNELAIRRLRVEAGIVPVVKQIDTVAAEFPAFTNYLYLTYNGSENDVDFNDQGVMVLGSGVYRIGSSVEFDWCSVRAIRTLREQGHKTVMVNENPETLSTDYDESDRLYFENSSLENVLDVYHLEGSSGVIISMGGQAPSNIALPLHRLNVNILGTSPEMIDMAENRYKFSRLLDHIDVDQPAWKELTSIEEATEFCAKVGYPVLVRPSYVLSGAAMNTVYSLDDLASYLNQAVEVSREHPVVITKYIENAKEIEMDAVAKNGVMVGHFISEHVENAGVHSGDATLILPPQDLDPETVRRIEEATRKIGNALNVTGPFNIQFIAKDNDIKVIECNVRASRSFPFVSKVMGVDLIEMATMAMIGKPFTEYPPVTIPKNYVGVKVPQFSFSRLSGADPVLGVEMASTGEVACFGRDRYEAYLKALISTGFKLPNKNILFSIGSFKEKLEMLPSIRKLHALGYNLFATAGTADYLKEHGLPVKYLEVLAGEEEDLKSEYSLTQHLANNLIDLYINLPSSNRFRRPANYMSKGYRTRRMAVDYQTPLVTNVKNAKILIEAIARHYSMNVMSIDCQTSYRAITLPGLINIAAFVPGLANPDSPDFQVVTKASIANGFSMVRIMPVGVDSFVTDSQALRTAQHNSSKGSYCDYNLSVAATSTNSERVGLITGEVGSLYIPFNHLSGNINKVATVTSHFASWPISKPIITDAKATDLASILLLASLHNRNIHVMSVTSKEDIGLIALSKEKGLKVTCDVSIYSLFLSTDDYPGCACLPSKKDQEMLWEHLDTIDVFSIGSLPYQVAGSKASPAVGIQDALPLLFTAVSDKRLTVDDVSSRLYDNPKRIFELHDQPDASVEIEFDRPYVFQANDVWSPFGGKTLKASVRRVIFQGKTSCLDGEMMPDALQGADMSAHRLTLPPPASPASKDILPISPRPDSAFARRLSFSATPVQRPSRFKPAAPDGYAVSASGDEFVPVPPIYPYPSHISPSLQQLLAQSAFKQKHILSVSQFNRTDLHLLFTVAQEMRLGVQRHGVLDILKGRVLTTLFFEPSTRTSASFDAAMQRLGGRTIPVATEHSSTQKGETLQDTVRTLGCYSDAIVLRHPADSSAATAAKFSPVPIINGGNGAMEHPTQAFLDLFTIREELGTVNDITITFTGDLKYGRTVHSLVKLLQFYEVRVQLVSPKTLAIPEEVRQQLISSGQLVLESTELTPEIISQSDVLYCTRVQKERFEDLSEYERLKNSFVIDNTMLKHAKSQMIVMHPLPRNQEICEEVDFDQRAAYFRQMKYGLYCRMALLALVMAP","length":2293,"molWeight":251880,"crc64":"ADCF1FCA8C0BA45B","md5":"2B88F70E7C5ECB9816537E5B792228F4"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":4,"COFACTOR":1,"PATHWAY":2,"SIMILARITY":4,"CAUTION":1},"countByFeatureType":{"Domain":3,"Active site":3},"uniParcId":"UPI0008009F54"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7P8L0","uniProtkbId":"A0A1B7P8L0_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":51,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX85375.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"Pentafunctional AROM polypeptide"}},"includes":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"3-dehydroquinate synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"DHQS"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"4.2.3.4"}]}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"3-phosphoshikimate 1-carboxyvinyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"2.5.1.19"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"5-enolpyruvylshikimate-3-phosphate synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"EPSP synthase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"EPSPS"}]}]},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"Shikimate kinase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"SK"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"2.7.1.71"}]}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"3-dehydroquinate dehydratase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"3-dehydroquinase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"4.2.1.10"}]}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"Shikimate dehydrogenase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"1.1.1.25"}]}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX85375.1"}],"value":"ACJ72_00264"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00054455"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-dehydroquinate = 3-dehydroshikimate + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21096"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:16630"},{"database":"ChEBI","id":"CHEBI:32364"}],"ecNumber":"4.2.1.10","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21256"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57701"},{"database":"ChEBI","id":"CHEBI:58702"},{"database":"ChEBI","id":"CHEBI:145989"}],"ecNumber":"2.5.1.19","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00044633"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:21257"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00044633"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21968"},{"database":"ChEBI","id":"CHEBI:32364"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58394"}],"ecNumber":"4.2.3.4","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"shikimate + ATP = 3-phosphoshikimate + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13121"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:36208"},{"database":"ChEBI","id":"CHEBI:145989"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.1.71","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048567"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17737"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16630"},{"database":"ChEBI","id":"CHEBI:36208"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"ecNumber":"1.1.1.25","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"Binds 2 Zn(2+) ions per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004842"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004811"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009948"}],"value":"Belongs to the EPSP synthase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"In the 2nd section; belongs to the EPSP synthase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"In the 3rd section; belongs to the shikimate kinase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"In the 4th section; belongs to the type-I 3-dehydroquinase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"In the C-terminal section; belongs to the shikimate dehydrogenase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000514"}],"value":"In the N-terminal section; belongs to the dehydroquinate synthase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"In the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX85375.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":189,"modifier":"EXACT"}},"description":"3-dehydroquinate synthase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01761"}]},{"type":"Domain","location":{"start":{"value":191,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"3-dehydroquinate synthase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF24621"}]},{"type":"Domain","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":836,"modifier":"EXACT"}},"description":"Enolpyruvate transferase","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00275"}]},{"type":"Domain","location":{"start":{"value":1293,"modifier":"EXACT"},"end":{"value":1373,"modifier":"EXACT"}},"description":"Shikimate dehydrogenase substrate binding N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF08501"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":384,"modifier":"EXACT"}},"description":"3-dehydroquinate synthase","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}]},{"type":"Region","location":{"start":{"value":397,"modifier":"EXACT"},"end":{"value":842,"modifier":"EXACT"}},"description":"EPSP synthase","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}]},{"type":"Region","location":{"start":{"value":1055,"modifier":"EXACT"},"end":{"value":1275,"modifier":"EXACT"}},"description":"3-dehydroquinase","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}]},{"type":"Region","location":{"start":{"value":1288,"modifier":"EXACT"},"end":{"value":1594,"modifier":"EXACT"}},"description":"Shikimate dehydrogenase","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}]},{"type":"Active site","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":260,"modifier":"EXACT"}},"description":"Proton acceptor; for 3-dehydroquinate synthase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}]},{"type":"Active site","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":275,"modifier":"EXACT"}},"description":"Proton acceptor; for 3-dehydroquinate synthase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}]},{"type":"Active site","location":{"start":{"value":824,"modifier":"EXACT"},"end":{"value":824,"modifier":"EXACT"}},"description":"For EPSP synthase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}]},{"type":"Active site","location":{"start":{"value":1178,"modifier":"EXACT"},"end":{"value":1178,"modifier":"EXACT"}},"description":"Proton acceptor; for 3-dehydroquinate dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}]},{"type":"Active site","location":{"start":{"value":1206,"modifier":"EXACT"},"end":{"value":1206,"modifier":"EXACT"}},"description":"Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}]},{"type":"Binding site","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":114,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":130,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":179,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":194,"modifier":"EXACT"},"end":{"value":197,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":194,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":250,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":271,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":271,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":287,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":287,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":356,"modifier":"EXACT"},"end":{"value":356,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58394"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate","id":"ChEBI:CHEBI:58394"}},{"type":"Binding site","location":{"start":{"value":869,"modifier":"EXACT"},"end":{"value":876,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022605"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023141"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0057","category":"Biological process","name":"Aromatic amino acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022777"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022833"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03143"}],"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX85375.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX85375.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000012","properties":[{"key":"ProteinId","value":"OAX85375.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"STRING","id":"1658172.A0A1B7P8L0","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"197068at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00053","properties":[{"key":"RectionId","value":"UER00085"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0003855","properties":[{"key":"GoTerm","value":"F:3-dehydroquinate dehydratase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003856","properties":[{"key":"GoTerm","value":"F:3-dehydroquinate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003866","properties":[{"key":"GoTerm","value":"F:3-phosphoshikimate 1-carboxyvinyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004764","properties":[{"key":"GoTerm","value":"F:shikimate 3-dehydrogenase (NADP+) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004765","properties":[{"key":"GoTerm","value":"F:shikimate kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009073","properties":[{"key":"GoTerm","value":"P:aromatic amino acid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009423","properties":[{"key":"GoTerm","value":"P:chorismate biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00502","properties":[{"key":"EntryName","value":"DHQase_I"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd08195","properties":[{"key":"EntryName","value":"DHQS"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01556","properties":[{"key":"EntryName","value":"EPSP_synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01065","properties":[{"key":"EntryName","value":"NAD_bind_Shikimate_DH"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00464","properties":[{"key":"EntryName","value":"SK"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.1090.10:FF:000007","properties":[{"key":"EntryName","value":"Pentafunctional AROM polypeptide"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000135","properties":[{"key":"EntryName","value":"Pentafunctional AROM polypeptide"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.1970:FF:000007","properties":[{"key":"EntryName","value":"Pentafunctional AROM polypeptide"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:001256","properties":[{"key":"EntryName","value":"Pentafunctional AROM polypeptide"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.65.10.10:FF:000007","properties":[{"key":"EntryName","value":"Pentafunctional AROM polypeptide"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.65.10.10:FF:000008","properties":[{"key":"EntryName","value":"Pentafunctional AROM polypeptide"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1970","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.1090.10","properties":[{"key":"EntryName","value":"Dehydroquinate synthase-like - alpha domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.65.10.10","properties":[{"key":"EntryName","value":"Enolpyruvate transferase domain"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.40.50.10860","properties":[{"key":"EntryName","value":"Leucine Dehydrogenase, chain A, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00210","properties":[{"key":"EntryName","value":"EPSP_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_03143","properties":[{"key":"EntryName","value":"Pentafunct_AroM"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00109","properties":[{"key":"EntryName","value":"Shikimate_kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR018508","properties":[{"key":"EntryName","value":"3-dehydroquinate_DH_AS"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR046346","properties":[{"key":"EntryName","value":"Aminoacid_DH-like_N_sf"}]},{"database":"InterPro","id":"IPR016037","properties":[{"key":"EntryName","value":"DHQ_synth_AroB"}]},{"database":"InterPro","id":"IPR030960","properties":[{"key":"EntryName","value":"DHQS/DOIS_N"}]},{"database":"InterPro","id":"IPR056179","properties":[{"key":"EntryName","value":"DHQS_C"}]},{"database":"InterPro","id":"IPR001381","properties":[{"key":"EntryName","value":"DHquinase_I"}]},{"database":"InterPro","id":"IPR001986","properties":[{"key":"EntryName","value":"Enolpyruvate_Tfrase_dom"}]},{"database":"InterPro","id":"IPR036968","properties":[{"key":"EntryName","value":"Enolpyruvate_Tfrase_sf"}]},{"database":"InterPro","id":"IPR006264","properties":[{"key":"EntryName","value":"EPSP_synthase"}]},{"database":"InterPro","id":"IPR023193","properties":[{"key":"EntryName","value":"EPSP_synthase_CS"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR008289","properties":[{"key":"EntryName","value":"Pentafunct_AroM"}]},{"database":"InterPro","id":"IPR013792","properties":[{"key":"EntryName","value":"RNA3'P_cycl/enolpyr_Trfase_a/b"}]},{"database":"InterPro","id":"IPR031322","properties":[{"key":"EntryName","value":"Shikimate/glucono_kinase"}]},{"database":"InterPro","id":"IPR013708","properties":[{"key":"EntryName","value":"Shikimate_DH-bd_N"}]},{"database":"InterPro","id":"IPR010110","properties":[{"key":"EntryName","value":"Shikimate_DH_AroM-type"}]},{"database":"InterPro","id":"IPR000623","properties":[{"key":"EntryName","value":"Shikimate_kinase/TSH1"}]},{"database":"InterPro","id":"IPR023000","properties":[{"key":"EntryName","value":"Shikimate_kinase_CS"}]},{"database":"NCBIfam","id":"TIGR01356","properties":[{"key":"EntryName","value":"aroA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01357","properties":[{"key":"EntryName","value":"aroB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01093","properties":[{"key":"EntryName","value":"aroD"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01809","properties":[{"key":"EntryName","value":"Shik-DH-AROM"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21090","properties":[{"key":"EntryName","value":"AROM/DEHYDROQUINATE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21090:SF5","properties":[{"key":"EntryName","value":"PENTAFUNCTIONAL AROM POLYPEPTIDE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01761","properties":[{"key":"EntryName","value":"DHQ_synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF24621","properties":[{"key":"EntryName","value":"DHQS_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01487","properties":[{"key":"EntryName","value":"DHquinase_I"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00275","properties":[{"key":"EntryName","value":"EPSP_synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08501","properties":[{"key":"EntryName","value":"Shikimate_dh_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01202","properties":[{"key":"EntryName","value":"SKI"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000514","properties":[{"key":"EntryName","value":"Pentafunct_AroM"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01100","properties":[{"key":"EntryName","value":"SHIKIMTKNASE"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53223","properties":[{"key":"EntryName","value":"Aminoacid dehydrogenase-like, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56796","properties":[{"key":"EntryName","value":"Dehydroquinate synthase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55205","properties":[{"key":"EntryName","value":"EPT/RTPC-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01028","properties":[{"key":"EntryName","value":"DEHYDROQUINASE_I"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00104","properties":[{"key":"EntryName","value":"EPSP_SYNTHASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00885","properties":[{"key":"EntryName","value":"EPSP_SYNTHASE_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01128","properties":[{"key":"EntryName","value":"SHIKIMATE_KINASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEDPTKISILGRESIVADFGIWRNYVAKDLLSSCPSSTYILISDTNLTPLYLAGFQAAFEDAAANLSPKPRLLTYEIPPGESSKSRETKAEIEDWMLTRQPPCGRDTVIIALGGGVIGDLIGFVAATYMRGVRFVQVPTTLLAMVDSSIGGKTAIDTPNGKNLIGAIWQPQRIYLDMEFLNTLPEREFINGMAEVIKTAAISSEEKFAALEKDAEIILAAVKSKNTPERPRFKGIEETLKRTILSSAEFKAQVVSADEREGGLRNLLNFGHSIGHAIEAILAPQVLHGECVSMGMVKEAELARHLGILNNVSVSRISKCLASYGLPTSLKDERIRRLTAGKHCSVEQLIAYMSVDKKNDGPKKKVVLLSAIGRTHEPRASTVSNEDIQIVLAPSLEVSPGVPKDLNVTCTPPGSKSISNRALVLAALGSGTCRLKNLLHSDDTEVMLNALERLGAATFHWENEGEVLVVNGNGGRMEASPEELYLGNAGTASRFLTTVATLAQKSSVDSSVLTGNARMKQRPIGDLVDALAANGAIIEYLENPKSLPLKIAASGGFAGGDINLAAKVSSQYVSSLLMCAPYAKKPVTLRLIGGKPISQPYIDMTTAMMRSFGIDVKKSETEEHTYHIPRGFYSNPAEYVVESDASSATYPLAVAAITGTSCTVPNIGSKSLQGDARFAVEVLQPMGCTVDQKDFSTTVTGPAGGILKPLPNIDMEPMTDAFLTASVLAAVARGQGSNHTTRIYGIANQRVKECNRIKAMKDELAKFGVTCREHDDGLEIDGIDRSTLLHPSDGVYCYDDHRVAMSFSVLSLVAPRPTLILEKECVGKTWPGWWDSLAQTFKVKLDGKEVEKKAEANGIATPAASIFIIGMRGAGKTTSGVWVSKALHRPFIDLDDELEKTEGMTIPEIIKQKGWGGFRAAELALLRRVMTEKPTGYIFACGGGVVETPEARKLLTQYHQTKGNVILIMRDIKEVMDFLNIDKTRPAYVEDMMSVWLRRKPWYQECSNVQYYSRLAGLDGMAQVLGAFNRFLKVITGEVDSLAEMRRKENTFFVSLTLPDLAPATHILKEVTLGSDAVELRVDLLKDPQSDNEIPSVDYVAEQISLLRSRVSVPLVFTVRTKSQGGRFPDDAYDAALQLYRLAIRMGSEFVDLEISFPEELLRTVTKMKGFSKIIASHHDPKGELSWVTGSWIQFYNKALQYGDVIKLVGVARSLDDNASLKKFKTWAEEKHDVPVIAINMGDKGQLSRMLNGFMTPVSHPSLPFKAAPGQLSAREIRKGLSLIGEIKAKKFAVIGNPVSASRSPAMHNALFRQMGLPHTYGTLETENPEVVKQFIRSPDFGGASVTIPLKLDIMPLLDEIAPEAESIGAVNTIVCAPPAPGDKSQKPRLIGRNTDWQGMVRCLSDAGAYAAATPTTTSAGLIIGGGGTARAAIFALQSMGYSPIYVVGRSPDKLANMTSTFPAGHDIRILEDATELESLPTVAIGTIPGDKPIEPHMREVLCKLFDLCENANTDAEQARGISTKRILLEMAYKPSITSLMQLASDSGWTVLPGLEVLVAQGVYQSEYWTDITPVYEDARNAVMGIPQIDDEIST","length":1594,"molWeight":173221,"crc64":"925DBC857D3AFC93","md5":"540C051E58B02DE5A9D0EAB7CA3B7B0F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":5,"COFACTOR":1,"PATHWAY":5,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":7,"CAUTION":2},"countByFeatureType":{"Domain":4,"Region":4,"Active site":5,"Binding site":22},"uniParcId":"UPI0007FFF46B"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NSE6","uniProtkbId":"A0A1B7NSE6_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2025-10-08","lastSequenceUpdateDate":"2016-11-02","entryVersion":39,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79718.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Phosphatidylserine decarboxylase proenzyme 1, mitochondrial"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"4.1.1.65"}]},"contains":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Phosphatidylserine decarboxylase 1 beta chain"}}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Phosphatidylserine decarboxylase 1 alpha chain"}}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"PSD1"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79718.1"}],"value":"ACJ72_05960"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20828"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57262"},{"database":"ChEBI","id":"CHEBI:64612"}],"ecNumber":"4.1.1.65","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"pyruvate","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:15361"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Binds 1 pyruvoyl group covalently per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005189"}],"value":"Lipid metabolism"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","molecule":"Phosphatidylserine decarboxylase 1 alpha chain","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Peripheral membrane protein","id":"SL-9903"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Intermembrane side","id":"SL-9912"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"Phosphatidylserine decarboxylase 1 beta chain","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Single-pass membrane protein","id":"SL-9904"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Intermembrane side","id":"SL-9912"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"value":"Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type I sub-subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79718.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":500,"modifier":"EXACT"}},"description":"Phosphatidylserine decarboxylase 1 beta chain","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"featureId":"PRO_5023397795"},{"type":"Chain","location":{"start":{"value":501,"modifier":"EXACT"},"end":{"value":552,"modifier":"EXACT"}},"description":"Phosphatidylserine decarboxylase 1 alpha chain","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"featureId":"PRO_5023397797"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"Mitochondrial matrix","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}]},{"type":"Topological domain","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":552,"modifier":"EXACT"}},"description":"Mitochondrial intermembrane","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}]},{"type":"Region","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":306,"modifier":"EXACT"},"end":{"value":333,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":269,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":221,"modifier":"EXACT"},"end":{"value":221,"modifier":"EXACT"}},"description":"Charge relay system; for autoendoproteolytic cleavage activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}]},{"type":"Active site","location":{"start":{"value":373,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"Charge relay system; for autoendoproteolytic cleavage activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}]},{"type":"Active site","location":{"start":{"value":501,"modifier":"EXACT"},"end":{"value":501,"modifier":"EXACT"}},"description":"Charge relay system; for autoendoproteolytic cleavage activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}]},{"type":"Active site","location":{"start":{"value":501,"modifier":"EXACT"},"end":{"value":501,"modifier":"EXACT"}},"description":"Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}]},{"type":"Site","location":{"start":{"value":500,"modifier":"EXACT"},"end":{"value":501,"modifier":"EXACT"}},"description":"Cleavage (non-hydrolytic); by autocatalysis","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}]},{"type":"Modified residue","location":{"start":{"value":501,"modifier":"EXACT"},"end":{"value":501,"modifier":"EXACT"}},"description":"Pyruvic acid (Ser); by autocatalysis","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022793"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0210","category":"Molecular function","name":"Decarboxylase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022516"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023098"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023209"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0594","category":"Biological process","name":"Phospholipid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023264"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-1208","category":"Biological process","name":"Phospholipid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023317"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0670","category":"Ligand","name":"Pyruvate"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03208"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79718.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79718.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000918","properties":[{"key":"ProteinId","value":"OAX79718.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NSE6","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NSE6","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"4330at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00558","properties":[{"key":"RectionId","value":"UER00616"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0004609","properties":[{"key":"GoTerm","value":"F:phosphatidylserine decarboxylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006646","properties":[{"key":"GoTerm","value":"P:phosphatidylethanolamine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016540","properties":[{"key":"GoTerm","value":"P:protein autoprocessing"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_03208","properties":[{"key":"EntryName","value":"PS_decarb_PSD_B_type1_euk"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003817","properties":[{"key":"EntryName","value":"PS_Dcarbxylase"}]},{"database":"InterPro","id":"IPR033177","properties":[{"key":"EntryName","value":"PSD-B"}]},{"database":"InterPro","id":"IPR033661","properties":[{"key":"EntryName","value":"PSD_type1_euk"}]},{"database":"NCBIfam","id":"TIGR00163","properties":[{"key":"EntryName","value":"PS_decarb"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10067","properties":[{"key":"EntryName","value":"PHOSPHATIDYLSERINE DECARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10067:SF6","properties":[{"key":"EntryName","value":"PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02666","properties":[{"key":"EntryName","value":"PS_Dcarbxylase"},{"key":"MatchStatus","value":"2"}]}],"sequence":{"value":"MFVRRAVVYRNPARFVRTWEGVVAELGPCRLNNSGAAPLSRRRPFSSSSGRWQHNGHEKGGSKEESFRSRLRTALGNTKVEWYPIPVGLGIGFLGFVQFFRSKKAEKERLERESGEEDGYGDVSGRRKVRPSGPWQVQVMSTLPLKAMSRIWGRFNELVIPYYLRVPGFKLYSWIFGVNLDEVAEPDLHTYPNLAAFFYRELKQGVRPLDPNPLAILSPSDGRILQFGMIENGEVEQVKGMTYSLDALLGHEKLSPGHEAVHPTKAPIHHSEHSEHSEHSSNKDPDNMAADENFAKMNGITYTLPTLLSGTGKGPPEEKPLDASMHSTTSSETKVRADLAKGETAWYKPTPTSNRSLFYVVIYLAPGDYHRFHSPVSWIVESRRHFAGELFSVSPYLQRTLPGLFTLNERIVLLGRWRWGFFSMTPVGATNVGSIKINFDSELRTNSLTTDTAADRAAAAAAKRGETYSGFAEATYRHASKTLAGHALQRGEEMGGFQLGSSIVLVFEAPLGKRPSFDMGWLGEHREGGWKWKIKKGQYVKVGQALGEVEDA","length":552,"molWeight":61644,"crc64":"4FC038E11F33975B","md5":"FF6E6DD67CB50BFAEDDD684128A3E5C0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":2,"PTM":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":2,"Topological domain":2,"Region":4,"Compositional bias":2,"Active site":4,"Site":1,"Modified residue":1},"uniParcId":"UPI00080240F4"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NTG4","uniProtkbId":"A0A1B7NTG4_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2025-04-02","lastSequenceUpdateDate":"2016-11-02","entryVersion":42,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX80069.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Phosphatidylserine decarboxylase proenzyme 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"4.1.1.65"}]},"contains":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Phosphatidylserine decarboxylase 2 beta chain"}}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Phosphatidylserine decarboxylase 2 alpha chain"}}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"PSD2"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX80069.1"}],"value":"ACJ72_05602"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20828"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:57262"},{"database":"ChEBI","id":"CHEBI:64612"}],"ecNumber":"4.1.1.65","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"pyruvate","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:15361"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Binds 1 pyruvoyl group covalently per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005189"}],"value":"Lipid metabolism"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit. Interacts with pstB2. This interaction may be a means to structurally tether the donor membrane (ER) harboring PstB2 to acceptor membranes (Golgi/endosomes) harboring PSD2 during PtdSer transport to the site of PtdEtn synthesis"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Golgi apparatus membrane","id":"SL-0134"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Peripheral membrane protein","id":"SL-9903"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Cytoplasmic side","id":"SL-9910"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Endosome membrane","id":"SL-0100"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Peripheral membrane protein","id":"SL-9903"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Cytoplasmic side","id":"SL-9910"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"The C2 domains have an essential, but non-catalytic function. They may facilitate interactions with other proteins and are required for lipid transport function"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"value":"Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type II sub-subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX80069.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":878,"modifier":"EXACT"}},"description":"Phosphatidylserine decarboxylase 2 beta chain","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"featureId":"PRO_5023210166"},{"type":"Chain","location":{"start":{"value":879,"modifier":"EXACT"},"end":{"value":956,"modifier":"EXACT"}},"description":"Phosphatidylserine decarboxylase 2 alpha chain","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"featureId":"PRO_5023210167"},{"type":"Domain","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":246,"modifier":"EXACT"}},"description":"C2","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50004"}]},{"type":"Domain","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":415,"modifier":"EXACT"}},"description":"EF-hand","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50222"}]},{"type":"Region","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":248,"modifier":"EXACT"},"end":{"value":327,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":466,"modifier":"EXACT"},"end":{"value":535,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":921,"modifier":"EXACT"},"end":{"value":956,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":253,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":312,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":475,"modifier":"EXACT"},"end":{"value":488,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":512,"modifier":"EXACT"},"end":{"value":530,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":921,"modifier":"EXACT"},"end":{"value":947,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":734,"modifier":"EXACT"},"end":{"value":734,"modifier":"EXACT"}},"description":"Charge relay system; for autoendoproteolytic cleavage activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}]},{"type":"Active site","location":{"start":{"value":792,"modifier":"EXACT"},"end":{"value":792,"modifier":"EXACT"}},"description":"Charge relay system; for autoendoproteolytic cleavage activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}]},{"type":"Active site","location":{"start":{"value":879,"modifier":"EXACT"},"end":{"value":879,"modifier":"EXACT"}},"description":"Charge relay system; for autoendoproteolytic cleavage activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}]},{"type":"Active site","location":{"start":{"value":879,"modifier":"EXACT"},"end":{"value":879,"modifier":"EXACT"}},"description":"Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}]},{"type":"Site","location":{"start":{"value":878,"modifier":"EXACT"},"end":{"value":879,"modifier":"EXACT"}},"description":"Cleavage (non-hydrolytic); by autocatalysis","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}]},{"type":"Modified residue","location":{"start":{"value":879,"modifier":"EXACT"},"end":{"value":879,"modifier":"EXACT"}},"description":"Pyruvic acid (Ser); by autocatalysis","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022793"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0210","category":"Molecular function","name":"Decarboxylase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022516"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023098"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023209"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0594","category":"Biological process","name":"Phospholipid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023264"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-1208","category":"Biological process","name":"Phospholipid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023317"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0670","category":"Ligand","name":"Pyruvate"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03209"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX80069.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX80069.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000806","properties":[{"key":"ProteinId","value":"OAX80069.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NTG4","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NTG4","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"67700at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00558","properties":[{"key":"RectionId","value":"UER00616"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0010008","properties":[{"key":"GoTerm","value":"C:endosome membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000139","properties":[{"key":"GoTerm","value":"C:Golgi membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005795","properties":[{"key":"GoTerm","value":"C:Golgi stack"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005509","properties":[{"key":"GoTerm","value":"F:calcium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004609","properties":[{"key":"GoTerm","value":"F:phosphatidylserine decarboxylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006646","properties":[{"key":"GoTerm","value":"P:phosphatidylethanolamine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016540","properties":[{"key":"GoTerm","value":"P:protein autoprocessing"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd04039","properties":[{"key":"EntryName","value":"C2_PSD"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.238.10:FF:000445","properties":[{"key":"EntryName","value":"Phosphatidylserine decarboxylase proenzyme 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.150","properties":[{"key":"EntryName","value":"C2 domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.238.10","properties":[{"key":"EntryName","value":"EF-hand"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00663","properties":[{"key":"EntryName","value":"PS_decarb_PSD_B_type2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR000008","properties":[{"key":"EntryName","value":"C2_dom"}]},{"database":"InterPro","id":"IPR035892","properties":[{"key":"EntryName","value":"C2_domain_sf"}]},{"database":"InterPro","id":"IPR011992","properties":[{"key":"EntryName","value":"EF-hand-dom_pair"}]},{"database":"InterPro","id":"IPR002048","properties":[{"key":"EntryName","value":"EF_hand_dom"}]},{"database":"InterPro","id":"IPR003817","properties":[{"key":"EntryName","value":"PS_Dcarbxylase"}]},{"database":"InterPro","id":"IPR033177","properties":[{"key":"EntryName","value":"PSD-B"}]},{"database":"InterPro","id":"IPR033179","properties":[{"key":"EntryName","value":"PSD_type2_pro"}]},{"database":"NCBIfam","id":"TIGR00163","properties":[{"key":"EntryName","value":"PS_decarb"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10067","properties":[{"key":"EntryName","value":"PHOSPHATIDYLSERINE DECARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10067:SF17","properties":[{"key":"EntryName","value":"PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00168","properties":[{"key":"EntryName","value":"C2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02666","properties":[{"key":"EntryName","value":"PS_Dcarbxylase"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00239","properties":[{"key":"EntryName","value":"C2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF49562","properties":[{"key":"EntryName","value":"C2 domain (Calcium/lipid-binding domain, CaLB)"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF47473","properties":[{"key":"EntryName","value":"EF-hand"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50004","properties":[{"key":"EntryName","value":"C2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50222","properties":[{"key":"EntryName","value":"EF_HAND_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGEFDIPLEDIFSAGTIQPQPQWYTLHCGRKAGKKRSDVSGEIQMQFTLFDPTNPTAEPAEIYQKFKALVSSSDDEDEIAAVVSPDTDDLEKEDETSDGTDDPTKPDVVEKRRRKLRIARLRRKSMAARAYQFSGSKDGVSGIVFMEIVKALDLPPERNMTRTSFDMDPFVVTSLGKKTLRTRVVRHNLNPVFEEKMVFQVMKHEQSYCIYFTVIDRDKLSGNDFVASSSFPLQTLVMAAPVADPETGLYKLPGPPSEPSPTPPASKSRFRLNLSRSSSATSLTKLTKPSLRSKSSATSLSSQSGQEQQSTTPPHALSETSPQLNIPAPANMVEEPEVPADEYGLKTYIIPITLKNKERWEDKHFPELHVKAKYMPYAALRQQFWRAMLKQYDADDSARISKVELTTMLDTLGSTLKESTVEGFFKRFAQENESSETADLTFDQAVICLEDTLQSLQKKSLGDKMKELERHHSSKNGPENENQSSSEDNVPEISLTLPSNTDPERPVVPTISRDERSTSGDSEDNLHPDDLGEDGGEEHVVEIRECPICHQPRLGKRSDADIITHIAICVSQDWRQVDNLVMGGFVTSSQAQRKWYSKVITKISYGGYKLGANSANILVQDRITGQINEERMSVYVRLGIRLLYKGLKSRDMEKKRIRKMLKSLSIKQGKKYDDPASASQILPFINFHHLDMSEVLLPLNEFKSFNEFFYRALKPGARPCSAPNNPRIIVSPADCRSVVFDRIDDATKIWVKGREFSVERLLGKAYPEDAKRYKNGALGIFRLAPQDYHRFHIPVDGVMGTPKTIEGEYYTVNPMAIRSALDVYGENVRIIVPIDTVEYGRVMVICVGAMMVGSTIITREGGERVKRGEELGYFKFGGSTLLVLFEPGKMCFDSDLVGNSLGALETLVRVGMSIGHHLDEPQYTPDMRKSEENITEKDREEAMRRIEGSLNNPSDG","length":956,"molWeight":107310,"crc64":"71CAB731BC6AC0C8","md5":"28C394A3265FB3F8505B8EE42B31F773"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"PTM":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":2,"Domain":2,"Region":4,"Compositional bias":6,"Active site":4,"Site":1,"Modified residue":1},"uniParcId":"UPI0007FDB825"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NTL8","uniProtkbId":"A0A1B7NTL8_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2016-11-02","entryVersion":43,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79967.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00038974"}],"value":"sterol 14alpha-demethylase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00038974"}],"value":"1.14.14.154"}]}},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79967.1"}],"value":"ACJ72_05706"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"32-hydroxyeburicol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxoeburicol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75431"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:194328"},{"database":"ChEBI","id":"CHEBI:194329"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052067"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75432"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052067"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75107"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:166681"},{"database":"ChEBI","id":"CHEBI:166806"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047379"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75108"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047379"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"32-oxoeburicol + reduced [NADPH--hemoprotein reductase] + O2 = 14-demethyleburicol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75435"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:194329"},{"database":"ChEBI","id":"CHEBI:194330"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051540"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75436"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051540"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75111"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:17813"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:166681"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048479"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75112"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048479"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta(14) steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:68068"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:138031"},{"database":"ChEBI","id":"CHEBI:176902"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049450"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:68069"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049450"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:68064"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:176901"},{"database":"ChEBI","id":"CHEBI:176902"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047587"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:68065"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047587"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 14alpha-methyl steroid + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = a Delta(14) steroid + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:54028"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:138029"},{"database":"ChEBI","id":"CHEBI:138031"}],"ecNumber":"1.14.14.154","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047702"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:54029"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047702"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:68060"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:138029"},{"database":"ChEBI","id":"CHEBI:176901"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048866"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:68061"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048866"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"eburicol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 14-demethyleburicol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75439"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:70315"},{"database":"ChEBI","id":"CHEBI:194330"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052625"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75440"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052625"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"eburicol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxyeburicol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75427"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:70315"},{"database":"ChEBI","id":"CHEBI:194328"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051806"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75428"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051806"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"lanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25286"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:16521"},{"database":"ChEBI","id":"CHEBI:17813"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"}],"ecNumber":"1.14.14.154","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047670"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:25287"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047670"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75103"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16521"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:166806"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049163"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75104"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049163"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"heme","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001971"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:30413"}}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004370"}],"value":"Membrane","id":"SL-0162"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010617"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000461"}],"value":"Belongs to the cytochrome P450 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79967.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Binding site","location":{"start":{"value":456,"modifier":"EXACT"},"end":{"value":456,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30413"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"ligand":{"name":"heme","id":"ChEBI:CHEBI:30413"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022617"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"id":"KW-0349","category":"Ligand","name":"Heme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023033"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000461"}],"id":"KW-0503","category":"Molecular function","name":"Monooxygenase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000461"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79967.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX79967.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000836","properties":[{"key":"ProteinId","value":"OAX79967.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NTL8","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NTL8","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1055148at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0020037","properties":[{"key":"GoTerm","value":"F:heme binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008398","properties":[{"key":"GoTerm","value":"F:sterol 14-demethylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"CDD","id":"cd11042","properties":[{"key":"EntryName","value":"CYP51-like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.630.10:FF:000033","properties":[{"key":"EntryName","value":"14-alpha sterol demethylase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.630.10","properties":[{"key":"EntryName","value":"Cytochrome P450"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050529","properties":[{"key":"EntryName","value":"CYP51A1-like"}]},{"database":"InterPro","id":"IPR001128","properties":[{"key":"EntryName","value":"Cyt_P450"}]},{"database":"InterPro","id":"IPR017972","properties":[{"key":"EntryName","value":"Cyt_P450_CS"}]},{"database":"InterPro","id":"IPR002403","properties":[{"key":"EntryName","value":"Cyt_P450_E_grp-IV"}]},{"database":"InterPro","id":"IPR036396","properties":[{"key":"EntryName","value":"Cyt_P450_sf"}]},{"database":"PANTHER","id":"PTHR24304:SF2","properties":[{"key":"EntryName","value":"24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR24304","properties":[{"key":"EntryName","value":"CYTOCHROME P450 FAMILY 7"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00067","properties":[{"key":"EntryName","value":"p450"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00465","properties":[{"key":"EntryName","value":"EP450IV"}]},{"database":"PRINTS","id":"PR00385","properties":[{"key":"EntryName","value":"P450"}]},{"database":"SUPFAM","id":"SSF48264","properties":[{"key":"EntryName","value":"Cytochrome P450"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00086","properties":[{"key":"EntryName","value":"CYTOCHROME_P450"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSWSNILTYGVLVFVSVVVLNLFYQLVFRLVNKTRPPLVFHWVPFLGNTISYGIDPYKFFFSCRQKHGDIFTFVLLGRQTTVYLGIKGNEFILNGKLKDVNAEEVYSPLTTPVFGPDVVYDCPNSKLIEQKKFIKYGLTQAALESHVPLIENEVQDYLATSPNFHGASGTVDISAAMAELTIFTAGSALQGEEVRAQLTTDFAVLYHDLDKGFSPINFMLPWAPLPHNKKRDAAHARMHAIYTGIIDKRRRAAMTNVSDSQKLDMIWNLMQCTYKSGQRLPDKEIANIMITLLMAGQHSSSSISSWIMLRLASEPAVAEELYQEQLANLPRAGPNESLAPLQYKDLDHLPLHQNVIRETLRLNTSIHSLLRKVKNPMSVPGTPYVIPASHNLLAAPGVTALSDEYFPNATVWNPHRWETQAPKENEKEEMVDYGYGATSKGTSSPYLPFGAGRHRCIGEKFAHLNLTVIVATMVRHLQFSNVEGQTGVPDTDYSSLFSGPKQPTRIQWERRAAKLG","length":516,"molWeight":57812,"crc64":"D38EC354A66EC558","md5":"A8783CB98AC85EA8EB3D735A6A845B64"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":12,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Transmembrane":1,"Binding site":1},"uniParcId":"UPI0007FE2B76"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NW89","uniProtkbId":"A0A1B7NW89_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":34,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81033.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00018074"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"value":"Autophagy-related protein 9"}}},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81033.1"}],"value":"ACJ72_04627"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00025503"}],"value":"Phospholipid scramblase involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Required for mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Different machineries are required for anterograde trafficking to the PAS during either the Cvt pathway or bulk autophagy and for retrograde trafficking"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"value":"Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:67920"},{"database":"ChEBI","id":"CHEBI:58088"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00024621"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:38663"},{"database":"ChEBI","id":"CHEBI:57262"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00024479"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:38571"},{"database":"ChEBI","id":"CHEBI:57643"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00024631"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:38895"},{"database":"ChEBI","id":"CHEBI:64612"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00024615"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00025904"}],"value":"Homotrimer; forms a homotrimer with a central pore that forms a path between the two membrane leaflets"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004439"}],"value":"Cytoplasmic vesicle membrane","id":"SL-0089"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004439"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004477"}],"value":"Endoplasmic reticulum membrane","id":"SL-0097"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004477"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004653"}],"value":"Golgi apparatus membrane","id":"SL-0134"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004653"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004511"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"value":"Preautophagosomal structure membrane","id":"SL-0221"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004511"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006185"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"value":"Belongs to the ATG9 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81033.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}]},{"type":"Transmembrane","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}]},{"type":"Transmembrane","location":{"start":{"value":339,"modifier":"EXACT"},"end":{"value":361,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}]},{"type":"Transmembrane","location":{"start":{"value":373,"modifier":"EXACT"},"end":{"value":391,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}]},{"type":"Region","location":{"start":{"value":500,"modifier":"EXACT"},"end":{"value":683,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":500,"modifier":"EXACT"},"end":{"value":515,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":518,"modifier":"EXACT"},"end":{"value":530,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":614,"modifier":"EXACT"},"end":{"value":627,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":628,"modifier":"EXACT"},"end":{"value":638,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":666,"modifier":"EXACT"},"end":{"value":676,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023006"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"id":"KW-0072","category":"Biological process","name":"Autophagy"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023329"}],"id":"KW-0968","category":"Cellular component","name":"Cytoplasmic vesicle"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023034"}],"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023055"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"id":"KW-0445","category":"Biological process","name":"Lipid transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364027"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81033.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81033.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000562","properties":[{"key":"ProteinId","value":"OAX81033.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NW89","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NW89","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"2020634at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005776","properties":[{"key":"GoTerm","value":"C:autophagosome"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0030659","properties":[{"key":"GoTerm","value":"C:cytoplasmic vesicle membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005789","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000139","properties":[{"key":"GoTerm","value":"C:Golgi membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0034045","properties":[{"key":"GoTerm","value":"C:phagophore assembly site membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0000422","properties":[{"key":"GoTerm","value":"P:autophagy of mitochondrion"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0034727","properties":[{"key":"GoTerm","value":"P:piecemeal microautophagy of the nucleus"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0034497","properties":[{"key":"GoTerm","value":"P:protein localization to phagophore assembly site"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0061709","properties":[{"key":"GoTerm","value":"P:reticulophagy"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR007241","properties":[{"key":"EntryName","value":"Autophagy-rel_prot_9"}]},{"database":"PANTHER","id":"PTHR13038","properties":[{"key":"EntryName","value":"APG9 AUTOPHAGY 9"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13038:SF10","properties":[{"key":"EntryName","value":"AUTOPHAGY-RELATED PROTEIN 9"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04109","properties":[{"key":"EntryName","value":"ATG9"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MWRWANVENLDNFLADVYDYFLGNGIKSILLSRVLNLLTLAFVVGFTVFLTSCIDYRLVPHGKTMSDIVVPHCVQEMSASSTFLLWLFSIFWIYQAVRYILDLRRLSHMHDFYHYLLGISDTDIQTISWQEIVSRLMALRDSNPSTAGAVSAKHRKFLGSQSKQRMDAHDIANRLMRKDNYLIALFNKDILDLTLPIPFLKSRQLFSRTLEWNLHFCILDYVFNDQGQVRTLFLKDTHRKALSDGLRRRFLISGLLNIFIAPFFLVYFLMHYFFRYFNEYQKNPSQIGSRQYTPLAEWKFREFNELRHLFQRRINMSYPFATRYIDQFPKDKTVQISRFVGFIAGALASVLALASVVDPELFLGFEISHDRTVLFYLGVFGTVWAVARGIVPEETLVFDPEFALREVIDFTHYQPAHWQGKLHSDEIRTEFAMLYQMKVVVFLEELLSMIFIPFVLWFSLPKCSDRLIDFFREFTVHVDGLGYVCSFAVFDFKKGTNVIPQNQDAQQEGPQNTGQPDLRNDYFSTKDGKMHRFHPPPAFPALASPPLMPEANYTGTGRPNRWDPQHRSVTRPTTAGAHAGGHSITPRTPRFGATAAAGAHHSPLPSVLLDPHHQPSTSGFRSVTRNVAHSRYRPKRQTHLTNDTIRDADESSRASQMLPGLDRHPSSQMTTESSGADDSRMEESWRMNLADDDGETDEETENVQDVVGGAGVLGLIHQFQKVSNDGRGGGGVGI","length":734,"molWeight":83982,"crc64":"0DF006BCCB8898A4","md5":"D6B0DC5F569444A3EEFFB6E02ACACDB2"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":4,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":2},"countByFeatureType":{"Transmembrane":4,"Region":1,"Compositional bias":5},"uniParcId":"UPI0007FFEBD4"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NWZ4","uniProtkbId":"A0A1B7NWZ4_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":33,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81291.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00039266"}],"value":"Cyclin-dependent kinase 1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012425"}],"value":"2.7.11.22"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00041293"}],"value":"Cell division protein kinase 1"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81291.1"}],"value":"ACJ72_04368"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00054329"}],"value":"Cyclin-dependent kinase that acts as a master regulator of the mitotic and meiotic cell cycles"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17989"},{"database":"Rhea","id":"RHEA-COMP:9863"},{"database":"Rhea","id":"RHEA-COMP:11604"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29999"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:83421"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10027"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17989"},{"database":"Rhea","id":"RHEA-COMP:9863"},{"database":"Rhea","id":"RHEA-COMP:11604"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29999"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:83421"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.22","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048367"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:46608"},{"database":"Rhea","id":"RHEA-COMP:11060"},{"database":"Rhea","id":"RHEA-COMP:11605"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30013"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:61977"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10027"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:46608"},{"database":"Rhea","id":"RHEA-COMP:11060"},{"database":"Rhea","id":"RHEA-COMP:11605"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30013"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:61977"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.22","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047811"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00064036"}],"value":"Forms a stable but non-covalent complex with a regulatory subunit (SUC1) and with a cyclin"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006485"}],"value":"Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"value":"Belongs to the protein kinase superfamily. Ser/Thr protein kinase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81291.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":307,"modifier":"EXACT"}},"description":"Protein kinase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50011"}]},{"type":"Active site","location":{"start":{"value":148,"modifier":"EXACT"},"end":{"value":148,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10027"}]},{"type":"Binding site","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10141"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10141"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023306"}],"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022618"}],"id":"KW-0132","category":"Biological process","name":"Cell division"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022777"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022776"}],"id":"KW-0498","category":"Biological process","name":"Mitosis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10141"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022553"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022527"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"id":"KW-0723","category":"Molecular function","name":"Serine/threonine-protein kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81291.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81291.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000505","properties":[{"key":"ProteinId","value":"OAX81291.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NWZ4","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NWZ4","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1732493at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0000307","properties":[{"key":"GoTerm","value":"C:cyclin-dependent protein kinase holoenzyme complex"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0030332","properties":[{"key":"GoTerm","value":"F:cyclin binding"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004693","properties":[{"key":"GoTerm","value":"F:cyclin-dependent protein serine/threonine kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0000082","properties":[{"key":"GoTerm","value":"P:G1/S transition of mitotic cell cycle"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0010389","properties":[{"key":"GoTerm","value":"P:regulation of G2/M transition of mitotic cell cycle"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0010468","properties":[{"key":"GoTerm","value":"P:regulation of gene expression"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0007165","properties":[{"key":"GoTerm","value":"P:signal transduction"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd07835","properties":[{"key":"EntryName","value":"STKc_CDK1_CdkB_like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.510.10:FF:000144","properties":[{"key":"EntryName","value":"Cyclin-dependent kinase 2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.200.20:FF:000027","properties":[{"key":"EntryName","value":"Putative Cyclin-dependent kinase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.200.20","properties":[{"key":"EntryName","value":"Phosphorylase Kinase, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.510.10","properties":[{"key":"EntryName","value":"Transferase(Phosphotransferase) domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050108","properties":[{"key":"EntryName","value":"CDK"}]},{"database":"InterPro","id":"IPR011009","properties":[{"key":"EntryName","value":"Kinase-like_dom_sf"}]},{"database":"InterPro","id":"IPR000719","properties":[{"key":"EntryName","value":"Prot_kinase_dom"}]},{"database":"InterPro","id":"IPR017441","properties":[{"key":"EntryName","value":"Protein_kinase_ATP_BS"}]},{"database":"InterPro","id":"IPR008271","properties":[{"key":"EntryName","value":"Ser/Thr_kinase_AS"}]},{"database":"PANTHER","id":"PTHR24056","properties":[{"key":"EntryName","value":"CELL DIVISION PROTEIN KINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR24056:SF254","properties":[{"key":"EntryName","value":"CYCLIN-DEPENDENT KINASE 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00069","properties":[{"key":"EntryName","value":"Pkinase"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00220","properties":[{"key":"EntryName","value":"S_TKc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56112","properties":[{"key":"EntryName","value":"Protein kinase-like (PK-like)"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00107","properties":[{"key":"EntryName","value":"PROTEIN_KINASE_ATP"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50011","properties":[{"key":"EntryName","value":"PROTEIN_KINASE_DOM"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00108","properties":[{"key":"EntryName","value":"PROTEIN_KINASE_ST"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MENYQKLEKIGEGTYGVVYKARDLTHPSRIVALKKIRLEAEDEGVPSTAIREISLLKEMHDPNIVRLLNIVHADGHKLYLVFEFLDLDLKKYMEALPVSEGGRGKALPNGSTLDMNRLGLGEAMVKKFMAQLVEGIRYCHSHRVLHRDLKPQNLLIDREGNLKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGGRQYSTGVDMWSVGAIFAEMCTRKPLFPGDSEIDEIFKIFKLLGTPDENTWPGVTSFPDFKVSFPKWKREATRKLVPGLERNGLDLLDAMLEYDPARRISAKQACMHPYFQAGSSAYSGRQRLPQYP","length":324,"molWeight":36779,"crc64":"40C73F24A264BB7D","md5":"671B4543FAEBC505A88F34E3C5285679"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":4,"SUBUNIT":1,"SIMILARITY":2,"CAUTION":1},"countByFeatureType":{"Domain":1,"Active site":1,"Binding site":1},"uniParcId":"UPI000801BA3E"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NXU3","uniProtkbId":"A0A1B7NXU3_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":39,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81591.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00017386"}],"value":"ATP-dependent DNA helicase CHL1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00044969"}],"value":"5.6.2.3"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00016387"}],"value":"ATP-dependent DNA helicase chl1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00029709"}],"value":"Chromosome loss protein 1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00044998"},{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00045008"}],"value":"DNA 5'-3' helicase CHL1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00080124"}],"value":"L29"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00035200"}],"value":"Large ribosomal subunit protein uL15"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81591.1"}],"value":"ACJ72_04065"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00045702"}],"value":"ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G(2)/M. May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres. Has a specific role in chromosome segregation during meiosis II"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP + H2O = ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13065"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"5.6.2.3","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048954"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction.","ecNumber":"5.6.2.3","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00093875"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"[4Fe-4S] cluster","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001966"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:49883"}}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004123"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008435"}],"value":"Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007320"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003888"}],"value":"Belongs to the universal ribosomal protein uL15 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81591.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":398,"modifier":"EXACT"}},"description":"Helicase ATP-binding","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51193"}]},{"type":"Region","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":904,"modifier":"EXACT"},"end":{"value":940,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00541"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023306"}],"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023125"}],"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022806"}],"id":"KW-0347","category":"Molecular function","name":"Helicase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00541"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023014"}],"id":"KW-0411","category":"Ligand","name":"Iron-sulfur"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00541"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023242"}],"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023274"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003888"}],"id":"KW-0687","category":"Molecular function","name":"Ribonucleoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022980"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU003888"}],"id":"KW-0689","category":"Molecular function","name":"Ribosomal protein"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81591.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81591.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000447","properties":[{"key":"ProteinId","value":"OAX81591.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NXU3","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NXU3","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"267079at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0015934","properties":[{"key":"GoTerm","value":"C:large ribosomal subunit"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0043139","properties":[{"key":"GoTerm","value":"F:5'-3' DNA helicase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016818","properties":[{"key":"GoTerm","value":"F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003735","properties":[{"key":"GoTerm","value":"F:structural constituent of ribosome"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006974","properties":[{"key":"GoTerm","value":"P:DNA damage response"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0034085","properties":[{"key":"GoTerm","value":"P:establishment of sister chromatid cohesion"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006412","properties":[{"key":"GoTerm","value":"P:translation"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd18788","properties":[{"key":"EntryName","value":"SF2_C_XPD"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.100.10.10:FF:000002","properties":[{"key":"EntryName","value":"60S ribosomal protein L27a"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:001372","properties":[{"key":"EntryName","value":"ATP-dependent DNA helicase chl1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:002774","properties":[{"key":"EntryName","value":"ATP-dependent DNA helicase chl1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.100.10.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"2"}]},{"database":"HAMAP","id":"MF_01341","properties":[{"key":"EntryName","value":"Ribosomal_uL15"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006555","properties":[{"key":"EntryName","value":"ATP-dep_Helicase_C"}]},{"database":"InterPro","id":"IPR045028","properties":[{"key":"EntryName","value":"DinG/Rad3-like"}]},{"database":"InterPro","id":"IPR002464","properties":[{"key":"EntryName","value":"DNA/RNA_helicase_DEAH_CS"}]},{"database":"InterPro","id":"IPR014013","properties":[{"key":"EntryName","value":"Helic_SF1/SF2_ATP-bd_DinG/Rad3"}]},{"database":"InterPro","id":"IPR006554","properties":[{"key":"EntryName","value":"Helicase-like_DEXD_c2"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR010614","properties":[{"key":"EntryName","value":"RAD3-like_helicase_DEAD"}]},{"database":"InterPro","id":"IPR013020","properties":[{"key":"EntryName","value":"Rad3/Chl1-like"}]},{"database":"InterPro","id":"IPR030878","properties":[{"key":"EntryName","value":"Ribosomal_uL15"}]},{"database":"InterPro","id":"IPR021131","properties":[{"key":"EntryName","value":"Ribosomal_uL15/eL18"}]},{"database":"InterPro","id":"IPR036227","properties":[{"key":"EntryName","value":"Ribosomal_uL15/eL18_sf"}]},{"database":"InterPro","id":"IPR001196","properties":[{"key":"EntryName","value":"Ribosomal_uL15_CS"}]},{"database":"NCBIfam","id":"TIGR00604","properties":[{"key":"EntryName","value":"rad3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11472:SF41","properties":[{"key":"EntryName","value":"ATP-DEPENDENT DNA HELICASE DDX11-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11472","properties":[{"key":"EntryName","value":"DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06733","properties":[{"key":"EntryName","value":"DEAD_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13307","properties":[{"key":"EntryName","value":"Helicase_C_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00828","properties":[{"key":"EntryName","value":"Ribosomal_L27A"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00488","properties":[{"key":"EntryName","value":"DEXDc2"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00491","properties":[{"key":"EntryName","value":"HELICc2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52080","properties":[{"key":"EntryName","value":"Ribosomal proteins L15p and L18e"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00690","properties":[{"key":"EntryName","value":"DEAH_ATP_HELICASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51193","properties":[{"key":"EntryName","value":"HELICASE_ATP_BIND_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00475","properties":[{"key":"EntryName","value":"RIBOSOMAL_L15"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPVEKNFHHPFIPYDIQRQFMQALYDCIQEGKVGIFESPTADGEPEWILEHSRREKTKSAREKRKQLEDRLAKVRAEEERQRKMFEDGNHPSKRRRLKVENAGSGQEDESQFELEDYYSGDEDRAVADTASTSDGVFSSHTLALLEKLKGGPVDTHADYEPDDEIHVFYCSRTHSQLTQFAHDLQRVLLPASVPPELGDDNIASTDNTPPVVDTLEESVKHLSLGSRKTLCINPKVRVLGNSTAINERCLEIQRPETPAEHKCPFVPNKENETLVNDFRDHTLAKVRDIEDIGKIGSKIGICPYYASRSIIKYSEIVTLPYPLLLQRSAREALNISLKNHVVIIDEAHNLMDAISNIHSVSMSLSQLQLAIYQLTTYARKYKTRLKGRNREYVTQVIRLVNSISDYLKTVLERKQPEEGSLLPSDIMTGKGVDQINPHKLSRYLHESKLARKVDGFIEQSSMESHADTNGTRSSVPVLFQVQSFLLSLMNPSSEGKLFYEISKNDVLLKYMLLDPTNHFREIVEDARTVILAGGTMSPMQDYADHLFPYLDPDQLKTFSYGHVIPPDNLTAIPMSKGAFNTEFDFTYGKRNEESMIIDLGRTIAALCRVIPDGVVVFFPSYDYLNQVLAIWKKVIPSTRSSIIDSIKRSKAVFHESQAEATSTEELLHDYSSVINAGSGGGALLLSVMGGKLSEGINFSDKLGRGVIVVGLPFPNIRSAVWQAKIRHVEHKAYELSSPGASSLGQRHEGQALSEESRRLKAKAAGREFYENACMRTVNQCIGRAIRHQGDYAAILMMDRRYCSPRIQSKLPNWIRQTLATGASEQAVEKTIGNISGFFRRKRAMNIITTGDNQSPVAHIKRERESRPESATIVVVVVVKSHLNAQHPHSLVVESSTVDKMPTRLSKTRKSRGHVSAGYGRVGKHRKHPGGRGMAGGQHHHRTNLDKYHPGYFGKVGMRHFHKLQNHYWKPVINLDKLWSLIPAETRDAYVKNQKTDTAPVLDLLPLGYSKVLGKGRLPEIPVVVRARYFSKEAERKIKEAGGVAELVA","length":1049,"molWeight":117962,"crc64":"D6545147244C7E14","md5":"1B82F1F46DD91132EA5DFDAFE745EF23"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":2,"CAUTION":1},"countByFeatureType":{"Domain":1,"Region":2,"Compositional bias":1},"uniParcId":"UPI00080048FE"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NYT3","uniProtkbId":"A0A1B7NYT3_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2016-11-02","entryVersion":46,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81931.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"value":"Lon protease homolog, mitochondrial"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"value":"3.4.21.53"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"value":"PIM1"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81931.1"}],"value":"ACJ72_03722"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"value":"ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Hydrolysis of proteins in presence of ATP.","ecNumber":"3.4.21.53","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10087"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"value":"Homohexamer or homoheptamer. Organized in a ring with a central cavity"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004305"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"value":"Mitochondrion matrix","id":"SL-0170"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01122"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000591"}],"value":"Belongs to the peptidase S16 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81931.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":423,"modifier":"EXACT"}},"description":"Lon N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51787"}]},{"type":"Domain","location":{"start":{"value":864,"modifier":"EXACT"},"end":{"value":1050,"modifier":"EXACT"}},"description":"Lon proteolytic","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51786"}]},{"type":"Region","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":803,"modifier":"EXACT"},"end":{"value":830,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"Gly residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":803,"modifier":"EXACT"},"end":{"value":822,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":956,"modifier":"EXACT"},"end":{"value":956,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10087"}]},{"type":"Active site","location":{"start":{"value":999,"modifier":"EXACT"},"end":{"value":999,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01122"}]},{"type":"Binding site","location":{"start":{"value":576,"modifier":"EXACT"},"end":{"value":583,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023125"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022825"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03120"}],"id":"KW-0720","category":"Molecular function","name":"Serine protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022946"}],"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81931.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX81931.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000383","properties":[{"key":"ProteinId","value":"OAX81931.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NYT3","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NYT3","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"2411602at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005759","properties":[{"key":"GoTerm","value":"C:mitochondrial matrix"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016887","properties":[{"key":"GoTerm","value":"F:ATP hydrolysis activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004176","properties":[{"key":"GoTerm","value":"F:ATP-dependent peptidase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0043565","properties":[{"key":"GoTerm","value":"F:sequence-specific DNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004252","properties":[{"key":"GoTerm","value":"F:serine-type endopeptidase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003697","properties":[{"key":"GoTerm","value":"F:single-stranded DNA binding"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0034599","properties":[{"key":"GoTerm","value":"P:cellular response to oxidative stress"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051131","properties":[{"key":"GoTerm","value":"P:chaperone-mediated protein complex assembly"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0141164","properties":[{"key":"GoTerm","value":"P:mitochondrial protein quality control"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0007005","properties":[{"key":"GoTerm","value":"P:mitochondrion organization"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0070407","properties":[{"key":"GoTerm","value":"P:oxidation-dependent protein catabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006508","properties":[{"key":"GoTerm","value":"P:proteolysis"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd19500","properties":[{"key":"EntryName","value":"RecA-like_Lon"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000021","properties":[{"key":"EntryName","value":"Lon protease homolog"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.8.60:FF:000113","properties":[{"key":"EntryName","value":"Lon protease homolog, mitochondrial"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.5.5270:FF:000001","properties":[{"key":"EntryName","value":"Lon protease homolog, mitochondrial"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.58.1480:FF:000003","properties":[{"key":"EntryName","value":"Lon protease homolog, mitochondrial"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.30.130.40:FF:000006","properties":[{"key":"EntryName","value":"Lon protease homolog, mitochondrial"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.230.10:FF:000015","properties":[{"key":"EntryName","value":"Lon protease homolog, mitochondrial"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.8.60","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.5.5270","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.58.1480","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.30.130.40","properties":[{"key":"EntryName","value":"LON domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_03120","properties":[{"key":"EntryName","value":"lonm_euk"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003593","properties":[{"key":"EntryName","value":"AAA+_ATPase"}]},{"database":"InterPro","id":"IPR003959","properties":[{"key":"EntryName","value":"ATPase_AAA_core"}]},{"database":"InterPro","id":"IPR004815","properties":[{"key":"EntryName","value":"Lon_bac/euk-typ"}]},{"database":"InterPro","id":"IPR054594","properties":[{"key":"EntryName","value":"Lon_lid"}]},{"database":"InterPro","id":"IPR008269","properties":[{"key":"EntryName","value":"Lon_proteolytic"}]},{"database":"InterPro","id":"IPR027065","properties":[{"key":"EntryName","value":"Lon_Prtase"}]},{"database":"InterPro","id":"IPR003111","properties":[{"key":"EntryName","value":"Lon_prtase_N"}]},{"database":"InterPro","id":"IPR046336","properties":[{"key":"EntryName","value":"Lon_prtase_N_sf"}]},{"database":"InterPro","id":"IPR027503","properties":[{"key":"EntryName","value":"Lonm_euk"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR008268","properties":[{"key":"EntryName","value":"Peptidase_S16_AS"}]},{"database":"InterPro","id":"IPR015947","properties":[{"key":"EntryName","value":"PUA-like_sf"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"NCBIfam","id":"TIGR00763","properties":[{"key":"EntryName","value":"lon"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43718","properties":[{"key":"EntryName","value":"LON PROTEASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43718:SF2","properties":[{"key":"EntryName","value":"LON PROTEASE HOMOLOG, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00004","properties":[{"key":"EntryName","value":"AAA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05362","properties":[{"key":"EntryName","value":"Lon_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22667","properties":[{"key":"EntryName","value":"Lon_lid"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02190","properties":[{"key":"EntryName","value":"LON_substr_bdg"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00830","properties":[{"key":"EntryName","value":"ENDOLAPTASE"}]},{"database":"SMART","id":"SM00382","properties":[{"key":"EntryName","value":"AAA"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00464","properties":[{"key":"EntryName","value":"LON"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF88697","properties":[{"key":"EntryName","value":"PUA domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51787","properties":[{"key":"EntryName","value":"LON_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51786","properties":[{"key":"EntryName","value":"LON_PROTEOLYTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01046","properties":[{"key":"EntryName","value":"LON_SER"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIRAQSLPWRAALRQASSRNLPFKFPPTPSSAYRFCVNSSLEAKSLLHPSPCALSSRSFSSSTARRKEKPQSPEPKEEKEEGTEAEKNQEEKPRPGRPSDGKDKTSDTPSPIPASTSPADGKPSGASAVGGGSPGSGSSDGGRRGRKNSAEKALQKATIPDVYPQVMAIPIARRPLFPGYYKAVTVRDPSVVSAIQEMMKRGQPYVGAFLFKDEAADRDVIEDIDEVHDVGVFAQITSVFPVHGTESGLTAVLYPHRRIKISSLIPPRDMSQKPETDQQIPNDAATEKQGDVVASFEEATLEQPPKDTPYEPTSFLHKYPVSIVHVENLTEEPYDKKNTMIRAVTNEIVNVFKDIANLNPLFRDQISTFSMSHSGNIMDEPANLADFAAAVSAGEVKELQDVLETMDIQERLSKALVVLKKELMNAQLQSKISKDVEAKIQKRQREYWLMEQMKGIRRELGIESDGKEKLVEKFKEKAEKLAMPEAVKKVFDEELNKLAHLEPAASEFNVTRNYLDWITQIPWGKRSPETFGIKNAMTVLDEDHYGLKDVKDRILEFIAVGKLRGTVEGKILCFVGPPGVGKTSIGKSIARALNRQYYRFSVGGLTDVAEIKGHRRTYVGALPGRVIQALKKCQTENPLILIDEVDKIGRGHQGDPSSALLELLDPEQNSSFLDHYMDVPVDLSKVLFVCTANMTDTIPRPLLDRMEMIELSGYVADEKMAIAERYLAPSAKEMSGLKDVDVMLEKDAIEELIKSYCRESGVRNLKKQIEKVYRKAALKIIQDLPEQEKDAEEVAVREEVRTALEQETKSEKSVAEENDKSPEPTPAPLVATHVPEDVHVSINKDNLKDYVGPPIFTSDRLYDVTPPGVAMGLAWTSMGGAALYVETILETALSPTSRPGLEQTGNLMTVMKESTVIAYSFAKSLMAKEFPENKFFERAKVHLHCPEGAVQKDGPSAGITMATSLLSLAFDHPLDPTIAMTGELTVTGKVLRIGGLREKTVAARRAGAKSIIFPADNMSDWLELPENIKEGISGHPVNWYSEVFDIVFRDLDKAQAQVMWKSQLQEVPSVIKSDHTKDEDE","length":1081,"molWeight":119693,"crc64":"40B0E817D552BFA3","md5":"78800524FACBA3BCA19E6E7AECFA2476"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":2,"Region":3,"Compositional bias":4,"Active site":2,"Binding site":1},"uniParcId":"UPI0007FEB421"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NZZ9","uniProtkbId":"A0A1B7NZZ9_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2016-11-02","entryVersion":41,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82339.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00038974"}],"value":"sterol 14alpha-demethylase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00038974"}],"value":"1.14.14.154"}]}},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82339.1"}],"value":"ACJ72_03309"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"32-hydroxyeburicol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxoeburicol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75431"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:194328"},{"database":"ChEBI","id":"CHEBI:194329"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052067"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75432"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052067"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75107"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:166681"},{"database":"ChEBI","id":"CHEBI:166806"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047379"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75108"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047379"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"32-oxoeburicol + reduced [NADPH--hemoprotein reductase] + O2 = 14-demethyleburicol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75435"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:194329"},{"database":"ChEBI","id":"CHEBI:194330"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051540"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75436"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051540"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75111"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:17813"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:166681"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048479"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75112"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048479"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta(14) steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:68068"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:138031"},{"database":"ChEBI","id":"CHEBI:176902"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049450"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:68069"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049450"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:68064"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:176901"},{"database":"ChEBI","id":"CHEBI:176902"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047587"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:68065"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047587"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 14alpha-methyl steroid + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = a Delta(14) steroid + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:54028"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:138029"},{"database":"ChEBI","id":"CHEBI:138031"}],"ecNumber":"1.14.14.154","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047702"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:54029"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047702"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:68060"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:138029"},{"database":"ChEBI","id":"CHEBI:176901"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048866"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:68061"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048866"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"eburicol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 14-demethyleburicol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75439"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:70315"},{"database":"ChEBI","id":"CHEBI:194330"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052625"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75440"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052625"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"eburicol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxyeburicol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75427"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:70315"},{"database":"ChEBI","id":"CHEBI:194328"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051806"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75428"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051806"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"lanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:25286"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:16521"},{"database":"ChEBI","id":"CHEBI:17813"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"}],"ecNumber":"1.14.14.154","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047670"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:25287"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047670"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75103"},{"database":"Rhea","id":"RHEA-COMP:11964"},{"database":"Rhea","id":"RHEA-COMP:11965"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16521"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:166806"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049163"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:75104"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049163"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"heme","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001971"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:30413"}}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004370"}],"value":"Membrane","id":"SL-0162"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010617"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000461"}],"value":"Belongs to the cytochrome P450 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82339.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Binding site","location":{"start":{"value":464,"modifier":"EXACT"},"end":{"value":464,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30413"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"ligand":{"name":"heme","id":"ChEBI:CHEBI:30413"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022617"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"id":"KW-0349","category":"Ligand","name":"Heme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023004"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"id":"KW-0408","category":"Ligand","name":"Iron"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602403-1"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023033"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000461"}],"id":"KW-0503","category":"Molecular function","name":"Monooxygenase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000461"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82339.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82339.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000319","properties":[{"key":"ProteinId","value":"OAX82339.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NZZ9","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NZZ9","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1055148at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0020037","properties":[{"key":"GoTerm","value":"F:heme binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008398","properties":[{"key":"GoTerm","value":"F:sterol 14-demethylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"CDD","id":"cd11042","properties":[{"key":"EntryName","value":"CYP51-like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.630.10:FF:000033","properties":[{"key":"EntryName","value":"14-alpha sterol demethylase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.630.10","properties":[{"key":"EntryName","value":"Cytochrome P450"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050529","properties":[{"key":"EntryName","value":"CYP51A1-like"}]},{"database":"InterPro","id":"IPR001128","properties":[{"key":"EntryName","value":"Cyt_P450"}]},{"database":"InterPro","id":"IPR017972","properties":[{"key":"EntryName","value":"Cyt_P450_CS"}]},{"database":"InterPro","id":"IPR002403","properties":[{"key":"EntryName","value":"Cyt_P450_E_grp-IV"}]},{"database":"InterPro","id":"IPR036396","properties":[{"key":"EntryName","value":"Cyt_P450_sf"}]},{"database":"PANTHER","id":"PTHR24304:SF2","properties":[{"key":"EntryName","value":"24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR24304","properties":[{"key":"EntryName","value":"CYTOCHROME P450 FAMILY 7"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00067","properties":[{"key":"EntryName","value":"p450"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00465","properties":[{"key":"EntryName","value":"EP450IV"}]},{"database":"PRINTS","id":"PR00385","properties":[{"key":"EntryName","value":"P450"}]},{"database":"SUPFAM","id":"SSF48264","properties":[{"key":"EntryName","value":"Cytochrome P450"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00086","properties":[{"key":"EntryName","value":"CYTOCHROME_P450"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGLLADVLARICAHYSTLSVGVLLLSSFATFLALSIVVNVLRQLLFKDPNEPPVVFHWFPVIGSTVSYGIDPYKFFLSSKEKYGDIFTFVLLGKKTTVFLGTKGNDFILNGKLKDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFVKYGLTSESLRSYVTLITDEFNQYLETSSAFQGDKGVLDVCKSIAEITIYTASRSLQGKEVRSKFDSSFAQLYHDLDMGFTAINFMLPWAPLPHNRKRDAAQQKMAKIYTDIIRQRREAGSKKDSEDMVWNLMSCVYKDGTPLPDIEIAHMMIALLMAGQHSSSSTLSWIVLRLASCPEVVEELYEEQKQVLGEDLPPVTYETLQKLTLNSHVIRETLRLHAPIHSILRAVKTPMPVDGTPYVIPTTHNLLAAPGVASRLPEHFPNPMTWDPHRWEASTMDKEDQNDEKLDYGYGLISKGANSPYLPFGSGRHRCIGEQFAYVQLGTLLVAIVRHLKLKRLDGETSLPDTDYSTLFSKPLGRPLIAWERRNPSKK","length":524,"molWeight":58994,"crc64":"DAE15F42798ED976","md5":"0C86AC729F800598D12B0BF9C0B5A065"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":12,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Transmembrane":1,"Binding site":1},"uniParcId":"UPI0007FFE183"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7P0V0","uniProtkbId":"A0A1B7P0V0_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2016-11-02","entryVersion":32,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82655.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00039850"}],"value":"Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012046"}],"value":"1.3.8.1"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00039036"}],"value":"1.3.8.5"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00042821"}],"value":"2-methyl branched chain acyl-CoA dehydrogenase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00041537"}],"value":"2-methylbutyryl-coenzyme A dehydrogenase"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82655.1"}],"value":"ACJ72_02992"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00094237"}],"value":"Short and branched chain specific acyl-CoA dehydrogenase that catalyzes the proR-proR stereospecific alpha,beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA). Among the different mitochondrial acyl-CoA dehydrogenases, acts specifically on short and branched chain acyl-CoA derivatives such as (S)-2-methylbutyryl-CoA as well as short straight chain acyl-CoAs such as butyryl-CoA (butanoyl-CoA). Plays an important role in the metabolism of L-isoleucine by catalyzing the dehydrogenation of 2-methylbutyryl-CoA, one of the steps of the L-isoleucine catabolic pathway. Can also act on valproyl-CoA, a metabolite of valproic acid, an antiepileptic drug"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2R)-2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = ethylacryloyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:65296"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"},{"database":"ChEBI","id":"CHEBI:156439"},{"database":"ChEBI","id":"CHEBI:156440"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048592"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:65297"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048592"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2S)-2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:48256"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57337"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"},{"database":"ChEBI","id":"CHEBI:88166"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049552"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:48257"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049552"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:43780"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57336"},{"database":"ChEBI","id":"CHEBI:57337"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"}],"ecNumber":"1.3.8.5","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048235"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:43781"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048235"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2-methylpropanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 2-methylpropenoyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:44180"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57338"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"},{"database":"ChEBI","id":"CHEBI:62500"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051903"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:44181"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051903"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a short-chain 2,3-saturated fatty acyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = a short-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:47196"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"},{"database":"ChEBI","id":"CHEBI:87487"},{"database":"ChEBI","id":"CHEBI:87488"}],"ecNumber":"1.3.8.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051002"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:47197"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051002"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24004"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57332"},{"database":"ChEBI","id":"CHEBI:57371"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049096"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:24005"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049096"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:43464"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"},{"database":"ChEBI","id":"CHEBI:62077"},{"database":"ChEBI","id":"CHEBI:62620"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049192"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:43465"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049192"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"valproyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-propylpent-2-enoyl-CoA + reduced [electron-transfer flavoprotein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:65344"},{"database":"Rhea","id":"RHEA-COMP:10685"},{"database":"Rhea","id":"RHEA-COMP:10686"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57692"},{"database":"ChEBI","id":"CHEBI:58307"},{"database":"ChEBI","id":"CHEBI:156457"},{"database":"ChEBI","id":"CHEBI:156458"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048307"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:65345"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048307"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"FAD","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001974"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362125"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:57692"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00037895"}],"value":"Amino-acid degradation; L-isoleucine degradation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005198"}],"value":"Lipid metabolism; mitochondrial fatty acid beta-oxidation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011881"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004305"}],"value":"Mitochondrion matrix","id":"SL-0170"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009347"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362125"}],"value":"Belongs to the acyl-CoA dehydrogenase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82655.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":172,"modifier":"EXACT"}},"description":"Acyl-CoA dehydrogenase/oxidase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02771"}]},{"type":"Domain","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"Acyl-CoA dehydrogenase/oxidase middle","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02770"}]},{"type":"Domain","location":{"start":{"value":284,"modifier":"EXACT"},"end":{"value":431,"modifier":"EXACT"}},"description":"Acyl-CoA dehydrogenase/oxidase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00441"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022990"}],"id":"KW-0007","category":"PTM","name":"Acetylation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022827"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362125"}],"id":"KW-0274","category":"Ligand","name":"FAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022832"}],"id":"KW-0276","category":"Biological process","name":"Fatty acid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022630"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362125"}],"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023098"}],"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023128"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362125"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022553"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022946"}],"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82655.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82655.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000274","properties":[{"key":"ProteinId","value":"OAX82655.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7P0V0","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7P0V0","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"10262177at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005759","properties":[{"key":"GoTerm","value":"C:mitochondrial matrix"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0050660","properties":[{"key":"GoTerm","value":"F:flavin adenine dinucleotide binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003853","properties":[{"key":"GoTerm","value":"F:short-chain 2-methyl fatty acyl-CoA dehydrogenase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0046395","properties":[{"key":"GoTerm","value":"P:carboxylic acid catabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0006631","properties":[{"key":"GoTerm","value":"P:fatty acid metabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"CDD","id":"cd01158","properties":[{"key":"EntryName","value":"SCAD_SBCAD"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.540.10:FF:000012","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase short/branched chain"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.140.10:FF:000002","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase short/branched chain"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.110.10:FF:000001","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase, mitochondrial"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.540.10","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase/oxidase, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.110.10","properties":[{"key":"EntryName","value":"Butyryl-CoA Dehydrogenase, subunit A, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.140.10","properties":[{"key":"EntryName","value":"Butyryl-CoA Dehydrogenase, subunit A, domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006089","properties":[{"key":"EntryName","value":"Acyl-CoA_DH_CS"}]},{"database":"InterPro","id":"IPR046373","properties":[{"key":"EntryName","value":"Acyl-CoA_Oxase/DH_mid-dom_sf"}]},{"database":"InterPro","id":"IPR036250","properties":[{"key":"EntryName","value":"AcylCo_DH-like_C"}]},{"database":"InterPro","id":"IPR009075","properties":[{"key":"EntryName","value":"AcylCo_DH/oxidase_C"}]},{"database":"InterPro","id":"IPR006091","properties":[{"key":"EntryName","value":"AcylCoA_DH/ox_M"}]},{"database":"InterPro","id":"IPR013786","properties":[{"key":"EntryName","value":"AcylCoA_DH/ox_N"}]},{"database":"InterPro","id":"IPR037069","properties":[{"key":"EntryName","value":"AcylCoA_DH/ox_N_sf"}]},{"database":"InterPro","id":"IPR009100","properties":[{"key":"EntryName","value":"AcylCoA_DH/oxidase_NM_dom_sf"}]},{"database":"PANTHER","id":"PTHR43884","properties":[{"key":"EntryName","value":"ACYL-COA DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43884:SF1","properties":[{"key":"EntryName","value":"SHORT_BRANCHED CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00441","properties":[{"key":"EntryName","value":"Acyl-CoA_dh_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02770","properties":[{"key":"EntryName","value":"Acyl-CoA_dh_M"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02771","properties":[{"key":"EntryName","value":"Acyl-CoA_dh_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF47203","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56645","properties":[{"key":"EntryName","value":"Acyl-CoA dehydrogenase NM domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00072","properties":[{"key":"EntryName","value":"ACYL_COA_DH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00073","properties":[{"key":"EntryName","value":"ACYL_COA_DH_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MASVARVLRPLARRPAVSILPAARNSVPSRTTFRCSRSFSATPRRRDVDLTSLTPTPITLLSETEAMMAESVAKFAQEQIGPKVRDMDEAETMDPVIVEQLFEQGLMGVEIPEEYGGAGMNFTSAIVGIEELARVDPSVSVMCDVHNTLVNTAIIRFADAEGKRKWLPKLATNTVGSFCLSEPVSGSDAFALQTKAVKTDSGYKINGSKMWITNSMEAGFFIVFANIDPSKGYKGITAFIVEKGTPGFSIAKKEKKLGIRASSTCVLNFDDVEIPKENLLGEEGQGYKYAINLLNEGRIGIAAQMTGLALGAWENAAKYIWNDRRQFGQLIGEFQGMQHQVAQAYTEIAAARALVYNAARKKEAGQDFVQDAAMAKLYASQVAGRVSGSAVEWMGGMGFVREGIAEKMFRDAKIGAIYEGTSNIQLQTIAKLLQKQYTQ","length":439,"molWeight":47818,"crc64":"1D29C301C5B964DD","md5":"7314DA07E0EE08DC0C462C394ACDEA53"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":8,"COFACTOR":1,"PATHWAY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":3},"uniParcId":"UPI0007FCAF48"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7P122","uniProtkbId":"A0A1B7P122_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":48,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82725.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00014008"}],"value":"Fatty acid synthase subunit alpha"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012948"}],"value":"1.1.1.100"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013191"}],"value":"2.3.1.41"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012878"}],"value":"2.3.1.86"}]}},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82725.1"}],"value":"ACJ72_02920"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17397"},{"database":"Rhea","id":"RHEA-COMP:9916"},{"database":"Rhea","id":"RHEA-COMP:9945"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:78776"},{"database":"ChEBI","id":"CHEBI:78827"}],"ecNumber":"1.1.1.100","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048508"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22836"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"Rhea","id":"RHEA-COMP:9916"},{"database":"Rhea","id":"RHEA-COMP:14125"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"},{"database":"ChEBI","id":"CHEBI:78776"},{"database":"ChEBI","id":"CHEBI:138651"}],"ecNumber":"2.3.1.41","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049541"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).","ecNumber":"2.3.1.86","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048237"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"pantetheine 4'-phosphate","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:47942"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007485"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000454"}],"value":"Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82725.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"Carrier","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50075"}]},{"type":"Domain","location":{"start":{"value":1118,"modifier":"EXACT"},"end":{"value":1655,"modifier":"EXACT"}},"description":"Ketosynthase family 3 (KS3)","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52004"}]},{"type":"Region","location":{"start":{"value":589,"modifier":"EXACT"},"end":{"value":627,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1348,"modifier":"EXACT"},"end":{"value":1370,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":601,"modifier":"EXACT"},"end":{"value":615,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1358,"modifier":"EXACT"},"end":{"value":1369,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":1300,"modifier":"EXACT"},"end":{"value":1300,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10022"}]},{"type":"Active site","location":{"start":{"value":1300,"modifier":"EXACT"},"end":{"value":1300,"modifier":"EXACT"}},"description":"For beta-ketoacyl synthase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000454-1"}]},{"type":"Binding site","location":{"start":{"value":1787,"modifier":"EXACT"},"end":{"value":1787,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000454-3"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":1788,"modifier":"EXACT"},"end":{"value":1788,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000454-3"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":1789,"modifier":"EXACT"},"end":{"value":1789,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000454-3"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Modified residue","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000454-4"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00258"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023160"}],"id":"KW-0275","category":"Biological process","name":"Fatty acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022832"}],"id":"KW-0276","category":"Biological process","name":"Fatty acid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022516"}],"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023160"}],"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000454-3"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000454-3"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022450"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000454"}],"id":"KW-0596","category":"PTM","name":"Phosphopantetheine"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022553"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR000454"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82725.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82725.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000263","properties":[{"key":"ProteinId","value":"OAX82725.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"STRING","id":"1658172.A0A1B7P122","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"4251012at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005835","properties":[{"key":"GoTerm","value":"C:fatty acid synthase complex"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004316","properties":[{"key":"GoTerm","value":"F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004315","properties":[{"key":"GoTerm","value":"F:3-oxoacyl-[acyl-carrier-protein] synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004312","properties":[{"key":"GoTerm","value":"F:fatty acid synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004321","properties":[{"key":"GoTerm","value":"F:fatty-acyl-CoA synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0008897","properties":[{"key":"GoTerm","value":"F:holo-[acyl-carrier-protein] synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0042759","properties":[{"key":"GoTerm","value":"P:long-chain fatty acid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0044550","properties":[{"key":"GoTerm","value":"P:secondary metabolite biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"CDD","id":"cd00828","properties":[{"key":"EntryName","value":"elong_cond_enzymes"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd08950","properties":[{"key":"EntryName","value":"KR_fFAS_SDR_c_like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.2490:FF:000001","properties":[{"key":"EntryName","value":"Fatty acid synthase subunit alpha"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.720:FF:000168","properties":[{"key":"EntryName","value":"Fatty acid synthase subunit alpha"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.25.70:FF:000001","properties":[{"key":"EntryName","value":"Fatty acid synthase subunit alpha"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.2490","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.47.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.25.70","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.10.140.1390","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.10.140.1410","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.10.250.1930","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.10.250.1940","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.470.20","properties":[{"key":"EntryName","value":"4'-phosphopantetheinyl transferase domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008278","properties":[{"key":"EntryName","value":"4-PPantetheinyl_Trfase_dom"}]},{"database":"InterPro","id":"IPR037143","properties":[{"key":"EntryName","value":"4-PPantetheinyl_Trfase_dom_sf"}]},{"database":"InterPro","id":"IPR016035","properties":[{"key":"EntryName","value":"Acyl_Trfase/lysoPLipase"}]},{"database":"InterPro","id":"IPR040899","properties":[{"key":"EntryName","value":"Fas_alpha_ACP"}]},{"database":"InterPro","id":"IPR047224","properties":[{"key":"EntryName","value":"FAS_alpha_su_C"}]},{"database":"InterPro","id":"IPR026025","properties":[{"key":"EntryName","value":"FAS_alpha_yeast"}]},{"database":"InterPro","id":"IPR041550","properties":[{"key":"EntryName","value":"FASI_helical"}]},{"database":"InterPro","id":"IPR050830","properties":[{"key":"EntryName","value":"Fungal_FAS"}]},{"database":"InterPro","id":"IPR014031","properties":[{"key":"EntryName","value":"KAS_C"}]},{"database":"InterPro","id":"IPR014030","properties":[{"key":"EntryName","value":"KAS_N"}]},{"database":"InterPro","id":"IPR018201","properties":[{"key":"EntryName","value":"Ketoacyl_synth_AS"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"InterPro","id":"IPR020841","properties":[{"key":"EntryName","value":"PKS_Beta-ketoAc_synthase_dom"}]},{"database":"InterPro","id":"IPR009081","properties":[{"key":"EntryName","value":"PP-bd_ACP"}]},{"database":"InterPro","id":"IPR004568","properties":[{"key":"EntryName","value":"Ppantetheine-prot_Trfase_dom"}]},{"database":"InterPro","id":"IPR016039","properties":[{"key":"EntryName","value":"Thiolase-like"}]},{"database":"NCBIfam","id":"TIGR00556","properties":[{"key":"EntryName","value":"pantethn_trn"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10982:SF21","properties":[{"key":"EntryName","value":"FATTY ACID SYNTHASE SUBUNIT BETA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10982","properties":[{"key":"EntryName","value":"MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01648","properties":[{"key":"EntryName","value":"ACPS"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18325","properties":[{"key":"EntryName","value":"Fas_alpha_ACP"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18314","properties":[{"key":"EntryName","value":"FAS_I_H"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00109","properties":[{"key":"EntryName","value":"ketoacyl-synt"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02801","properties":[{"key":"EntryName","value":"Ketoacyl-synt_C"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000454","properties":[{"key":"EntryName","value":"FAS_yeast_alpha"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56214","properties":[{"key":"EntryName","value":"4'-phosphopantetheinyl transferase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52151","properties":[{"key":"EntryName","value":"FabD/lysophospholipase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53901","properties":[{"key":"EntryName","value":"Thiolase-like"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS50075","properties":[{"key":"EntryName","value":"CARRIER"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00606","properties":[{"key":"EntryName","value":"KS3_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52004","properties":[{"key":"EntryName","value":"KS3_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRPEVEQELAHTLLVELLAYQFASPVRWIETQDVILQEKRTERIVEIGPADTLGGMARRTIASKYEAYDAATSVQRQILCYNKDAKEIYYDVDPVEDEPEPAPAAAAAAAAAAAASSPSTASNAPAAPVVASAPSPSSGPAVAVADAPVLALDIVRVLVAQKLKKPLADIPVTKAIKDLVGGKSTLQNEILGDLGKEFGSTPEKPEDTPLDELGAAMQPTFNGQLGKHSLSLVARLVSSKMPGGFNITAVRKYLETRWGLQSGRQDGLLLLALTMEPPSRLGSEADAKAYLDDVANKYAANAGINLSTAPGGGEGGNGGGGMMMDPAALDALTKDQRAFFKQQLELTARYLKRDLRAGDKAFLISQETQATLQAQLDLWHAEHGDTYASGIEPSFDPLKARVYDSSWNWARQDGLSMYYDVIFGRLKVVDREIVSQCIRLMNRSNPLLVEFMQYHIDNCPTERGETYKLAKELGQQLIENCKEVLNEKPVYKDVAIPTGPQTVIDSRGNIKYSEIPRGSARKLEHYVRAMAEGGPISEYSNRTKVQNDLKSVYKLIRKQHKLSKSSQLQFNALYREVLRALSMNENQIMPQENGHAKKGSRNSSRSRINGAIVNGNGNGNGHVNGRPGKVETVPFLHLKKKEASGWEYSKKLTGIYLDGLESAARAGVTFHGKNALMTGAGAGSIGAAVLQGMISGGAKVVVTTSRFSREVTEYYQTMYTRYGARGSQLVVVPFNQGSKQDVEALVNYIYDPKNGLGWDLDYIVPFAAISENGREIDNIDSKSELAHRLMLTNIFRLLGYVKSQKQERGFETRPAQVILPLSPNHGTFGNDGLYSESKLGLETLFNRWYSESWANYLTICGAVIGWTRCTGLMNANDVIAEGVEKLGVRTFSQQEMAFNLLGLMAPAIVDLCQNQPVFADLNGGLQFLPDLNLLMKKLHNEVKETSTVRQAVTKETAQENKVVNGEDSEALYKKVTTVPRANIKFEFPNLPDWKEDIEPLNAKLKGMVNLDKVVVVTGFAEIGPWGNSRTRWEMEAYGKFSLEGCIEMAWLMGLIKNHNGPIKGKPYSGWVDAKTGEPVHDKDVKAKYEKHILEHSGIRLIEPELFNGYDPNKKQLLQEIVLQEDLESFEASKETANEFKREHGEKVEVFEIPDSGEYTVRLLKGATLMIPKALKFDRVVAGQIPTGWNPKHYGIPDDIISQVDPVTLYVLVCTVESLLASGITDPYEFYKYVHISEVGNCIGSGIGGSHALRGMYKDRYLDKPLQKDILQESFINTMSAWVNMLLLSSTGPIKTPVGACATAVESVDIGYETIVEGKARVCFVGGFDDFQEEGSYEFANMQATSNAEKEFAHGRTPQEMSRPTTTTRSGFMESQGCGMQLLMSAQLALDMGVPIYGIIGLTTTATDKIGRSVPAPGKGVLTTARENPGKFPSPLLDINYRRRQLDLRRAQIKQWQESELLYLNDEVEAIKAQATEPFNADEYMQERVEHIKREAVRQEKDAAFSLGNNFWRQDPRIAPLRGALATWGLTIDDLDVASFHGTATVANDKNECDVLCQQMKHLGRQKGNAMMGIFQKYLTGHAKGAAGGFMINGALQVLNSGLVPGNRNADNIDKIMEKFDYIVYPSRSLQTDGVKAFSVTSFGFGQKGTQAIGIHPKYLFATLDRATFEGYKKKVEARQKKAYRYFHNGLINNSLFVAKDKSPYADEMESKVFLNPDSRVVLNEKTDTLGYATIPSHGNGAPNTKNNTRSTISIPHPSEDPQTLHTRQMVESLAKATASENSKVGVDVESIAAINIENETFVERNFTEEEQRYCRAAPSPQASFAGRWSAKEAVFKSLGVVGKGAGAAMRDIEILSSGGEVGSGKGGPVVVVSVFCPP","length":1878,"molWeight":206288,"crc64":"E48496D42C614873","md5":"3B08666E44AA1DCD03348E6F032E1A9E"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":3,"COFACTOR":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":2,"Region":2,"Compositional bias":2,"Active site":2,"Binding site":3,"Modified residue":1},"uniParcId":"UPI0007FD0E1B"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7P126","uniProtkbId":"A0A1B7P126_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":44,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82726.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00068309"}],"value":"Fatty acid synthase subunit beta"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012996"}],"value":"1.3.1.9"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013256"}],"value":"2.3.1.38"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013258"}],"value":"2.3.1.39"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012878"}],"value":"2.3.1.86"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012480"}],"value":"3.1.2.14"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013167"}],"value":"4.2.1.59"}]}},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82726.1"}],"value":"ACJ72_02921"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00058855"}],"value":"Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(9Z)-octadecenoyl-[ACP] + H2O = (9Z)-octadecenoate + holo-[ACP] + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15057"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"Rhea","id":"RHEA-COMP:9924"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30823"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78783"}],"ecNumber":"3.1.2.14","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048536"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13097"},{"database":"Rhea","id":"RHEA-COMP:9925"},{"database":"Rhea","id":"RHEA-COMP:9945"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:78784"},{"database":"ChEBI","id":"CHEBI:78827"}],"ecNumber":"4.2.1.59","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001055"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10240"},{"database":"Rhea","id":"RHEA-COMP:9925"},{"database":"Rhea","id":"RHEA-COMP:9926"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:78784"},{"database":"ChEBI","id":"CHEBI:78785"}],"ecNumber":"1.3.1.9","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048572"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).","ecNumber":"2.3.1.86","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048237"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41788"},{"database":"Rhea","id":"RHEA-COMP:9621"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78446"}],"ecNumber":"2.3.1.38","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048835"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:41792"},{"database":"Rhea","id":"RHEA-COMP:9623"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57384"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78449"}],"ecNumber":"2.3.1.39","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048462"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005179"}],"value":"Secondary metabolite biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00033756"}],"value":"[Alpha(6)beta(6)] hexamers of two multifunctional subunits (alpha and beta)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010009"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR005562"}],"value":"Belongs to the fungal fatty acid synthetase subunit beta family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82726.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":1693,"modifier":"EXACT"},"end":{"value":2014,"modifier":"EXACT"}},"description":"Malonyl-CoA:ACP transacylase (MAT)","evidences":[{"evidenceCode":"ECO:0000259","source":"SMART","id":"SM00827"}]},{"type":"Active site","location":{"start":{"value":286,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"For acetyltransferase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR005562-1"}]},{"type":"Active site","location":{"start":{"value":1837,"modifier":"EXACT"},"end":{"value":1837,"modifier":"EXACT"}},"description":"For malonyltransferase activity","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR005562-1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023160"}],"id":"KW-0275","category":"Biological process","name":"Fatty acid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022832"}],"id":"KW-0276","category":"Biological process","name":"Fatty acid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR005562"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022516"}],"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023098"}],"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023239"}],"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023268"}],"id":"KW-0511","category":"Molecular function","name":"Multifunctional enzyme"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR005562"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR005562"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR005562"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"PIRNR","id":"PIRNR005562"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82726.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX82726.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000263","properties":[{"key":"ProteinId","value":"OAX82726.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"STRING","id":"1658172.A0A1B7P126","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"5417908at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005835","properties":[{"key":"GoTerm","value":"C:fatty acid synthase complex"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019171","properties":[{"key":"GoTerm","value":"F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004313","properties":[{"key":"GoTerm","value":"F:[acyl-carrier-protein] S-acetyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004314","properties":[{"key":"GoTerm","value":"F:[acyl-carrier-protein] S-malonyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004318","properties":[{"key":"GoTerm","value":"F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004312","properties":[{"key":"GoTerm","value":"F:fatty acid synthase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016297","properties":[{"key":"GoTerm","value":"F:fatty acyl-[ACP] hydrolase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0004321","properties":[{"key":"GoTerm","value":"F:fatty-acyl-CoA synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0042759","properties":[{"key":"GoTerm","value":"P:long-chain fatty acid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"CDD","id":"cd03447","properties":[{"key":"EntryName","value":"FAS_MaoC"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.1050.120:FF:000001","properties":[{"key":"EntryName","value":"Fatty acid synthase beta subunit dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.930.70:FF:000001","properties":[{"key":"EntryName","value":"Fatty acid synthase beta subunit dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.10.129.10:FF:000017","properties":[{"key":"EntryName","value":"Fatty acid synthase beta subunit dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000078","properties":[{"key":"EntryName","value":"Fatty acid synthase beta subunit dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.1120.100:FF:000001","properties":[{"key":"EntryName","value":"Fatty acid synthase beta subunit dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.366.10:FF:000006","properties":[{"key":"EntryName","value":"Fatty acid synthase beta subunit dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.366.10:FF:000007","properties":[{"key":"EntryName","value":"Fatty acid synthase beta subunit dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.10.129.10:FF:000015","properties":[{"key":"EntryName","value":"Fatty acid synthase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.70:FF:000169","properties":[{"key":"EntryName","value":"Fatty acid synthase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.3330:FF:000001","properties":[{"key":"EntryName","value":"Fatty acid synthase subunit beta dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.366.10:FF:000003","properties":[{"key":"EntryName","value":"Fatty acid synthase subunit beta dehydratase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.1050.120","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.930.70","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1120.100","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.3330","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.10.140.1400","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.10.60.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.20.240.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.10.129.10","properties":[{"key":"EntryName","value":"Hotdog Thioesterase"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"3.40.366.10","properties":[{"key":"EntryName","value":"Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"},{"key":"MatchStatus","value":"3"}]},{"database":"InterPro","id":"IPR001227","properties":[{"key":"EntryName","value":"Ac_transferase_dom_sf"}]},{"database":"InterPro","id":"IPR014043","properties":[{"key":"EntryName","value":"Acyl_transferase_dom"}]},{"database":"InterPro","id":"IPR016035","properties":[{"key":"EntryName","value":"Acyl_Trfase/lysoPLipase"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR039569","properties":[{"key":"EntryName","value":"FAS1-like_DH_region"}]},{"database":"InterPro","id":"IPR016452","properties":[{"key":"EntryName","value":"Fas1/AflB-like"}]},{"database":"InterPro","id":"IPR013565","properties":[{"key":"EntryName","value":"Fas1/AflB-like_central"}]},{"database":"InterPro","id":"IPR041099","properties":[{"key":"EntryName","value":"FAS1_N"}]},{"database":"InterPro","id":"IPR040883","properties":[{"key":"EntryName","value":"FAS_meander"}]},{"database":"InterPro","id":"IPR003965","properties":[{"key":"EntryName","value":"Fatty_acid_synthase"}]},{"database":"InterPro","id":"IPR050830","properties":[{"key":"EntryName","value":"Fungal_FAS"}]},{"database":"InterPro","id":"IPR029069","properties":[{"key":"EntryName","value":"HotDog_dom_sf"}]},{"database":"InterPro","id":"IPR002539","properties":[{"key":"EntryName","value":"MaoC-like_dom"}]},{"database":"InterPro","id":"IPR032088","properties":[{"key":"EntryName","value":"SAT"}]},{"database":"PANTHER","id":"PTHR10982:SF21","properties":[{"key":"EntryName","value":"FATTY ACID SYNTHASE SUBUNIT BETA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10982","properties":[{"key":"EntryName","value":"MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00698","properties":[{"key":"EntryName","value":"Acyl_transf_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08354","properties":[{"key":"EntryName","value":"Fas1-AflB-like_hel"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13452","properties":[{"key":"EntryName","value":"FAS1_DH_region"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22235","properties":[{"key":"EntryName","value":"FAS1_thioest_ins"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17951","properties":[{"key":"EntryName","value":"FAS_meander"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17828","properties":[{"key":"EntryName","value":"FAS_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01575","properties":[{"key":"EntryName","value":"MaoC_dehydratas"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16073","properties":[{"key":"EntryName","value":"SAT"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF005562","properties":[{"key":"EntryName","value":"FAS_yeast_beta"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01483","properties":[{"key":"EntryName","value":"FASYNTHASE"}]},{"database":"SMART","id":"SM00827","properties":[{"key":"EntryName","value":"PKS_AT"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52151","properties":[{"key":"EntryName","value":"FabD/lysophospholipase-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF54637","properties":[{"key":"EntryName","value":"Thioesterase/thiol ester dehydrase-isomerase"},{"key":"MatchStatus","value":"2"}]}],"sequence":{"value":"MYGTSTGPQTGINTPRSSQSLRPLILSHGSLEFSFLVPTSLHFHAHQLKDSFSASLPAPTDELAQDDEPSSVQELVARYLGYVASQVEEGEDDAQGSYLEVLKLVLNEFERAFMRGNDVHAIASILPGISAKKLLVVQAYYAARAAANRPTKPHDSALLRAAADGNAQLYCVFGGQGNIEEYFDELREIYTTYPSFVEDLISSSAELLQSLSRDPDAIKLYSKGMDIVRWLQDRDSQPDTDYLVSAPVSLPLIGLVQLAHFMTTCKVMGKQPGEILERFSGTTGHSQGIVTAALISTATTWDSFHKASRNALTMLFWIGMRSQQAYPRTSLAPSILQDSIENGEGVPTPMLSIRDLARAAVQEHIDATNQHLPQDRHIAISLINSARNFVVTGPPISLYGLNLRLRKVKAPTGLDQNRVPYTQRKVRFVNRFLPITAPFHSQYLTSAFEQITEDLEDIVIPSKSLRIPVFNTNTGEDLRSKSEDIVPALIRMITRDPVNWENATVFPKATHVLDFGPGGISGLGVLTNRNKDGTGVRVILAGAMDGTNAEVGYKPELFDRDEEHAVKYAVDWVKEHGPRLVKTSVGQTFVDTKMSRLLGVPPVMVAGMTPTTVPWDFVAATMNAGYQIELAGGGYYNAKSMTEALNKIEKNIPPGRGITVNLIYVNPRAMGWQIPLIGKLRADGVPIEGLTIGAGVPSIEVANEYIQTLGIKHIGFKPGSMDAIQQVINIAKANPTFPVIMQWTGGRGGGHHSFEDFHQPILQMYSRIRRCDNIVLVAGSGFGGSEDTYPYLTGSWSRQFGFPPMPFDGCLFGSRMMTAKEAHTSRAAKQAIAEAEGVDDNRWEDTYNGPAGGVITVRSEMGEPIHKLATRGVRFWHEMDQKIFSLDKAKRLPELKKQRDYIIKKLNADFQKVWFGRNSQGDTVDLEDMTYTEVVHRMVDLMYVKHESRWIDESLKRLAGDFIRRVEERFTSVDGQPSLLQNYSQLDTPYPAVDKILSAYPEAASQLISAQDVQHFLLLCQRRGQKPVPFVPSLDENFEFWFKKDSLWQSEDLEAVVGQDVGRVCILQGPMAVKYSNTIDEPVKNILDGIHNAHIKGLVRDVYGGNESNVPVVEYFGGQLLLEADAETEIDGLTISEDATKLSYRLSPSTSASLPDLDHWLHLLAGKTYSWRYAMFTTEVFIQGQKFQTNPMKRILAPTPGMFVEIQYPNDARKTLISVREPSTAGKLVKTVEVRTNGEREILLTLFEGRTAEGGVVPLCFRFTYHPETGYAPIREVMDDRNDRIKEFYYRIWFGDRNVPFDTPATAIFDGGKATVTSQAIADFVHAVGNTGEAFVDRPGKEVFAPMDFAIVVGWKAITKPIFPRVIDGDLLKLVHLSNGFRMIPGSEPLKVGDVLETTAQVNAVINQDSGKMVEVCGTIRREGKPIMEVTSQFLYRGAYNDFETTFQRKEEVPMQVHLSSSKDIAVLQSKEWFRLDEPDVDLLGQTLTFRLQSLVRFKNQNVLSSVQTIGQVLLELPTKEVIQVASVEYEAGTSHGNPVIDYLQRHGSAIEQPVHFENPIPLSGKTPLILRAPASNETYARVSGDYNPIHVSRVFSSYANLPGTITHGMYTSAAVRSLVETWAAENNIGRVRSFHASLVGMVLPNDDLVVVLQHVGMVAGRKIIKVEASNQATEEKVLLGEAEVEQPVSAYVFTGQGSQEQGMGMDLYNSSPVAKEVWDRADKHFIENYGLSIINIVKNNPQELTIHFGGPRGKIIRQNYMSMTFETVNADGSIKSEKIFKDVDENSTSYTYRSPTGLLSATQFTQPALTLMEKASFEDMRSKGLVQRDSSFAGHSLGEYSALAALADVMPIESLVSVVFYRGLTMQVAVERDELGRSNYSMCAVNPSRISKTFNEQALQYVVENIAEQTGWLLEIVNYNIANMQYVCAGDLRALDTLTNVLNLLKAQKIDITTLMSTMSLDDLRAHLVEIITSCSQLTNSKPKPINLERGFATIPLKGIDVPFHSTFLRSGVKPFRSFLLKKINKTTIDPSKLIGKYIPNVTAKPFELTKEYFEDVYKLTNSPRIGAVLANWGRYEDGEK","length":2082,"molWeight":231261,"crc64":"CFE65DA279F9B6B9","md5":"82E791D7365C0118D787402F504F2611"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":6,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Active site":2},"uniParcId":"UPI0007FD1D60"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7P3V2","uniProtkbId":"A0A1B7P3V2_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":47,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX83722.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022255"}],"value":"Histone acetyltransferase ESA1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013184"}],"value":"2.3.1.48"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00074445"}],"value":"Histone acetyltransferase esa1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031065"},{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00075313"}],"value":"protein 2-hydroxyisobutyryltransferase ESA1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031553"},{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00076782"}],"value":"protein acetyltransferase ESA1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031133"},{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00077673"}],"value":"protein crotonyltransferase ESA1"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX83722.1"}],"value":"ACJ72_01922"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00045805"}],"value":"Catalytic component of the NuA4 histone acetyltransferase (HAT) complex which is involved in epigenetic transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4, H3, H2B, H2A and H2A variant H2A.Z. Acetylates histone H4 to form H4K5ac, H4K8ac, H4K12ac and H4K16ac, histone H3 to form H3K14ac, and histone H2A to form H2AK4ac and H2AK7ac. The NuA4 complex is involved in the DNA damage response and is required for chromosome segregation. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) through homologous recombination. Recruitment to promoters depends on H3K4me. Also acetylates non-histone proteins. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA) or (2E)-butenoyl-CoA (crotonyl-CoA), and is able to mediate protein 2-hydroxyisobutyrylation and crotonylation, respectively"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2E)-butenoyl-CoA + L-lysyl-[protein] = N(6)-(2E)-butenoyl-L-lysyl-[protein] + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:53908"},{"database":"Rhea","id":"RHEA-COMP:9752"},{"database":"Rhea","id":"RHEA-COMP:13707"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29969"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57332"},{"database":"ChEBI","id":"CHEBI:137954"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047752"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:53909"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047752"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2-hydroxyisobutanoyl-CoA + L-lysyl-[protein] = N(6)-(2-hydroxyisobutanoyl)-L-lysyl-[protein] + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24180"},{"database":"Rhea","id":"RHEA-COMP:9752"},{"database":"Rhea","id":"RHEA-COMP:15921"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29969"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:131780"},{"database":"ChEBI","id":"CHEBI:144968"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047557"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:24181"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047557"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21992"},{"database":"Rhea","id":"RHEA-COMP:9845"},{"database":"Rhea","id":"RHEA-COMP:11338"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29969"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"},{"database":"ChEBI","id":"CHEBI:61930"}],"ecNumber":"2.3.1.48","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048940"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:21993"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048940"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:45948"},{"database":"Rhea","id":"RHEA-COMP:9752"},{"database":"Rhea","id":"RHEA-COMP:10731"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29969"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"},{"database":"ChEBI","id":"CHEBI:61930"}],"ecNumber":"2.3.1.48","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:45948"},{"database":"Rhea","id":"RHEA-COMP:9752"},{"database":"Rhea","id":"RHEA-COMP:10731"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29969"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"},{"database":"ChEBI","id":"CHEBI:61930"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047787"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:45949"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047787"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011353"}],"value":"Component of the NuA4 histone acetyltransferase complex"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004123"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010107"}],"value":"Belongs to the MYST (SAS/MOZ) family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX83722.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":505,"modifier":"EXACT"}},"description":"MYST-type HAT","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51726"}]},{"type":"Region","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":405,"modifier":"EXACT"},"end":{"value":405,"modifier":"EXACT"}},"description":"Proton donor/acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR602717-51"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}]},{"type":"Binding site","location":{"start":{"value":409,"modifier":"EXACT"},"end":{"value":409,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57288"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}],"ligand":{"name":"acetyl-CoA","id":"ChEBI:CHEBI:57288"}},{"type":"Modified residue","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"N6-acetyllysine; by autocatalysis","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022990"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}],"id":"KW-0007","category":"PTM","name":"Acetylation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023159"}],"id":"KW-0010","category":"Molecular function","name":"Activator"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022853"}],"id":"KW-0156","category":"Molecular function","name":"Chromatin regulator"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022763"}],"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023204"}],"id":"KW-0234","category":"Biological process","name":"DNA repair"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023242"}],"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023163"}],"id":"KW-0804","category":"Biological process","name":"Transcription"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023015"}],"id":"KW-0805","category":"Biological process","name":"Transcription regulation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}],"id":"KW-0862","category":"Ligand","name":"Zinc"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01063"}],"id":"KW-0863","category":"Domain","name":"Zinc-finger"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX83722.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX83722.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000143","properties":[{"key":"ProteinId","value":"OAX83722.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7P3V2","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7P3V2","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"787137at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0000785","properties":[{"key":"GoTerm","value":"C:chromatin"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0003682","properties":[{"key":"GoTerm","value":"F:chromatin binding"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004402","properties":[{"key":"GoTerm","value":"F:histone acetyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0106226","properties":[{"key":"GoTerm","value":"F:peptide 2-hydroxyisobutyryltransferase activity"},{"key":"GoEvidenceType","value":"IEA:RHEA"}]},{"database":"GO","id":"GO:0140064","properties":[{"key":"GoTerm","value":"F:peptide crotonyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:RHEA"}]},{"database":"GO","id":"GO:0003712","properties":[{"key":"GoTerm","value":"F:transcription coregulator activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006357","properties":[{"key":"GoTerm","value":"P:regulation of transcription by RNA polymerase II"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd04301","properties":[{"key":"EntryName","value":"NAT_SF"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.10.10:FF:000022","properties":[{"key":"EntryName","value":"Histone acetyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.60.60:FF:000001","properties":[{"key":"EntryName","value":"Histone acetyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.630.30:FF:000002","properties":[{"key":"EntryName","value":"Histone acetyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.30.30.140","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.630.30","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.60.60","properties":[{"key":"EntryName","value":"N-acetyl transferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.10.10","properties":[{"key":"EntryName","value":"Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR016181","properties":[{"key":"EntryName","value":"Acyl_CoA_acyltransferase"}]},{"database":"InterPro","id":"IPR016197","properties":[{"key":"EntryName","value":"Chromo-like_dom_sf"}]},{"database":"InterPro","id":"IPR000953","properties":[{"key":"EntryName","value":"Chromo/chromo_shadow_dom"}]},{"database":"InterPro","id":"IPR002717","properties":[{"key":"EntryName","value":"HAT_MYST-type"}]},{"database":"InterPro","id":"IPR050603","properties":[{"key":"EntryName","value":"MYST_HAT"}]},{"database":"InterPro","id":"IPR025995","properties":[{"key":"EntryName","value":"Tudor-knot"}]},{"database":"InterPro","id":"IPR036388","properties":[{"key":"EntryName","value":"WH-like_DNA-bd_sf"}]},{"database":"InterPro","id":"IPR040706","properties":[{"key":"EntryName","value":"Zf-MYST"}]},{"database":"PANTHER","id":"PTHR10615","properties":[{"key":"EntryName","value":"HISTONE ACETYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10615:SF218","properties":[{"key":"EntryName","value":"HISTONE ACETYLTRANSFERASE ESA1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01853","properties":[{"key":"EntryName","value":"MOZ_SAS"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11717","properties":[{"key":"EntryName","value":"Tudor-knot"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17772","properties":[{"key":"EntryName","value":"zf-MYST"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00298","properties":[{"key":"EntryName","value":"CHROMO"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55729","properties":[{"key":"EntryName","value":"Acyl-CoA N-acyltransferases (Nat)"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54160","properties":[{"key":"EntryName","value":"Chromo domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51726","properties":[{"key":"EntryName","value":"MYST_HAT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGVSDASQEAPGTPDPVEKGFATINTLRIGVKAFVEKDGEHRKAEILSMRQRKGGPSFYVHYVDFNKRLDEWVPASRIDLTQEVEWPQPEKPEKKKTTGAAAPKVQPKNAPKRVRADSRDLSTAPDLLAGKNLNISKSSRPSKAGGKENGEDVTPLSLSLLGSEAVSAVGTPGLDGESEDIEMLDAVDAEAKQAIKQEEDKIAGEIAREEEIEKLRTSGSMTQNPTEIHRVRNLTHIQMGKFEIEPWYFSPYPAHFTDADMVYIDEFCLSYFDQKRSFERHRAKCSLVHPPGNEIYRDDNVSFFEVDGRRQRTWCRNLCLLSKLFLDHKTLYYDVDPFLFYCMTTRDEHGCHLVGYFSKEKESAEGYNVACILTLPQYQRRGFGRLLIAFSYELSKRENKLGSPEKPLSDLGLLGYRQYWRETIVDILMEPGRETISESELANLSAMTEKDVHETLVVLNLLRYNKGNWIIVLTDAVIEQHKKRLEKEKIKGARKIDPARLQWKPPVFTASSRTWNW","length":517,"molWeight":58920,"crc64":"A56FEF6F42B02AA2","md5":"3F343A533D71613C5764CCB290B1ABE0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":5,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":2},"countByFeatureType":{"Domain":1,"Region":1,"Active site":1,"Binding site":1,"Modified residue":1},"uniParcId":"UPI0007FD2B43"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7P546","uniProtkbId":"A0A1B7P546_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":47,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84140.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}],"value":"Phospholipid-transporting ATPase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}],"value":"7.6.2.1"}]}},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84140.1"}],"value":"ACJ72_01485"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.","ecNumber":"7.6.2.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00034036"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:38567"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57262"},{"database":"ChEBI","id":"CHEBI:456216"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051303"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:38568"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00051303"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:38583"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57643"},{"database":"ChEBI","id":"CHEBI:456216"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052223"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:38584"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00052223"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:66132"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:64612"},{"database":"ChEBI","id":"CHEBI:456216"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049128"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:66133"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049128"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:66036"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:22801"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:456216"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00050913"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:66037"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00050913"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-3"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004651"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004651"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008109"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}],"value":"Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84140.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]},{"type":"Transmembrane","location":{"start":{"value":513,"modifier":"EXACT"},"end":{"value":538,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]},{"type":"Transmembrane","location":{"start":{"value":558,"modifier":"EXACT"},"end":{"value":581,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]},{"type":"Transmembrane","location":{"start":{"value":1129,"modifier":"EXACT"},"end":{"value":1148,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]},{"type":"Transmembrane","location":{"start":{"value":1160,"modifier":"EXACT"},"end":{"value":1181,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]},{"type":"Transmembrane","location":{"start":{"value":1211,"modifier":"EXACT"},"end":{"value":1232,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]},{"type":"Transmembrane","location":{"start":{"value":1252,"modifier":"EXACT"},"end":{"value":1270,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]},{"type":"Transmembrane","location":{"start":{"value":1277,"modifier":"EXACT"},"end":{"value":1297,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]},{"type":"Transmembrane","location":{"start":{"value":1317,"modifier":"EXACT"},"end":{"value":1337,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}]},{"type":"Domain","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"P-type ATPase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF16209"}]},{"type":"Domain","location":{"start":{"value":1097,"modifier":"EXACT"},"end":{"value":1346,"modifier":"EXACT"}},"description":"P-type ATPase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF16212"}]},{"type":"Region","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":298,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1373,"modifier":"EXACT"},"end":{"value":1469,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":259,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1413,"modifier":"EXACT"},"end":{"value":1432,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":627,"modifier":"EXACT"},"end":{"value":627,"modifier":"EXACT"}},"description":"4-aspartylphosphate intermediate","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-1"}]},{"type":"Binding site","location":{"start":{"value":627,"modifier":"EXACT"},"end":{"value":627,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":627,"modifier":"EXACT"},"end":{"value":627,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-3"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":628,"modifier":"EXACT"},"end":{"value":628,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":629,"modifier":"EXACT"},"end":{"value":629,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":629,"modifier":"EXACT"},"end":{"value":629,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-3"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":761,"modifier":"EXACT"},"end":{"value":761,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":802,"modifier":"EXACT"},"end":{"value":802,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":825,"modifier":"EXACT"},"end":{"value":825,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":860,"modifier":"EXACT"},"end":{"value":860,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":940,"modifier":"EXACT"},"end":{"value":940,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":941,"modifier":"EXACT"},"end":{"value":941,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":942,"modifier":"EXACT"},"end":{"value":942,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":1045,"modifier":"EXACT"},"end":{"value":1045,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":1051,"modifier":"EXACT"},"end":{"value":1051,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":1071,"modifier":"EXACT"},"end":{"value":1071,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-3"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":1074,"modifier":"EXACT"},"end":{"value":1074,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":1075,"modifier":"EXACT"},"end":{"value":1075,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":1075,"modifier":"EXACT"},"end":{"value":1075,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-3"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022475"}],"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023055"}],"id":"KW-0445","category":"Biological process","name":"Lipid transport"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-3"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-3"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR606539-2"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362033"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84140.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84140.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000101","properties":[{"key":"ProteinId","value":"OAX84140.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"STRING","id":"1658172.A0A1B7P546","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"377733at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0070867","properties":[{"key":"GoTerm","value":"C:mating projection tip membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016887","properties":[{"key":"GoTerm","value":"F:ATP hydrolysis activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0140351","properties":[{"key":"GoTerm","value":"F:glycosylceramide flippase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0090554","properties":[{"key":"GoTerm","value":"F:phosphatidylcholine floppase activity"},{"key":"GoEvidenceType","value":"IEA:RHEA"}]},{"database":"GO","id":"GO:0140346","properties":[{"key":"GoTerm","value":"F:phosphatidylserine flippase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0090556","properties":[{"key":"GoTerm","value":"F:phosphatidylserine floppase activity"},{"key":"GoEvidenceType","value":"IEA:RHEA"}]},{"database":"GO","id":"GO:0099040","properties":[{"key":"GoTerm","value":"P:ceramide translocation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0006897","properties":[{"key":"GoTerm","value":"P:endocytosis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0007163","properties":[{"key":"GoTerm","value":"P:establishment or maintenance of cell polarity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"CDD","id":"cd02073","properties":[{"key":"EntryName","value":"P-type_ATPase_APLT_Dnf-like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.1110.10:FF:000048","properties":[{"key":"EntryName","value":"Phospholipid-transporting ATPase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.1000:FF:000108","properties":[{"key":"EntryName","value":"Phospholipid-transporting ATPase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.1000:FF:000001","properties":[{"key":"EntryName","value":"Phospholipid-transporting ATPase IC"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1110.10","properties":[{"key":"EntryName","value":"Calcium-transporting ATPase, cytoplasmic domain N"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.70.150.10","properties":[{"key":"EntryName","value":"Calcium-transporting ATPase, cytoplasmic transduction domain A"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1000","properties":[{"key":"EntryName","value":"HAD superfamily/HAD-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR023299","properties":[{"key":"EntryName","value":"ATPase_P-typ_cyto_dom_N"}]},{"database":"InterPro","id":"IPR018303","properties":[{"key":"EntryName","value":"ATPase_P-typ_P_site"}]},{"database":"InterPro","id":"IPR023298","properties":[{"key":"EntryName","value":"ATPase_P-typ_TM_dom_sf"}]},{"database":"InterPro","id":"IPR008250","properties":[{"key":"EntryName","value":"ATPase_P-typ_transduc_dom_A_sf"}]},{"database":"InterPro","id":"IPR036412","properties":[{"key":"EntryName","value":"HAD-like_sf"}]},{"database":"InterPro","id":"IPR023214","properties":[{"key":"EntryName","value":"HAD_sf"}]},{"database":"InterPro","id":"IPR006539","properties":[{"key":"EntryName","value":"P-type_ATPase_IV"}]},{"database":"InterPro","id":"IPR032631","properties":[{"key":"EntryName","value":"P-type_ATPase_N"}]},{"database":"InterPro","id":"IPR001757","properties":[{"key":"EntryName","value":"P_typ_ATPase"}]},{"database":"InterPro","id":"IPR032630","properties":[{"key":"EntryName","value":"P_typ_ATPase_c"}]},{"database":"InterPro","id":"IPR044492","properties":[{"key":"EntryName","value":"P_typ_ATPase_HD_dom"}]},{"database":"NCBIfam","id":"TIGR01652","properties":[{"key":"EntryName","value":"ATPase-Plipid"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01494","properties":[{"key":"EntryName","value":"ATPase_P-type"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR24092:SF180","properties":[{"key":"EntryName","value":"PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR24092","properties":[{"key":"EntryName","value":"PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13246","properties":[{"key":"EntryName","value":"Cation_ATPase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16212","properties":[{"key":"EntryName","value":"PhoLip_ATPase_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16209","properties":[{"key":"EntryName","value":"PhoLip_ATPase_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00119","properties":[{"key":"EntryName","value":"CATATPASE"}]},{"database":"SFLD","id":"SFLDG00002","properties":[{"key":"EntryName","value":"C1.7:_P-type_atpase_like"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDS00003","properties":[{"key":"EntryName","value":"Haloacid_Dehalogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"SFLD","id":"SFLDF00027","properties":[{"key":"EntryName","value":"p-type_atpase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81653","properties":[{"key":"EntryName","value":"Calcium ATPase, transduction domain A"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81665","properties":[{"key":"EntryName","value":"Calcium ATPase, transmembrane domain M"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56784","properties":[{"key":"EntryName","value":"HAD-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81660","properties":[{"key":"EntryName","value":"Metal cation-transporting ATPase, ATP-binding domain N"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00154","properties":[{"key":"EntryName","value":"ATPASE_E1_E2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAVVQFGAEDEATVSKPVRRMRWATQRHAGAGGSRKRRSIMDRLHRRTGSKEEKRKSIASSLPHGDGSTVDEGSVDTSNIRRIFCNVPLTDDMKDEDGRFMPNYSRNKIRTAKYTPLSFVPKNLWFQCHNIANIYFLFTIILAIFPIFGATNPGLSAVPLISILTITAIKDAVEDWRRTVLDNELNNSPIHRLVEWNNVNSVEDSVSLWRRAKKATTKSVVKFWRMIIALKKGGKVQDPEEEIRAGQRPSIDTRRTHRTSTYSFRQSFQLNNGDSQEDGGAIQMTPVPSPNLDQHNAQQDGQSFGMLRASTDFLSPDPHQKLDPRNTRREYGSVLNKGRQTPQTARFKRDYWKNVQVGDFVRIYNEEPIPADVVVFSTSDPDGACYVETKNLDGETNLKVRQALHCTRAVKHARDCEKAEFTIESEAPHPNLYEYNGAIKWTQHDANSPDGPGRDMVEPITINNILLRGCSIQNTEWVLGMVIFTGLQTKIMLNSGATPSKRAKLARDLNWNVIYNFIILFFMCFISGIVQGVTWGQGRNSLNLFEFGSYGGSPPLDGFVTFWAAVILYQNLVPISLYISLEIVRTAQAIFIHSDTFMFYDKLGYPCTPKSWNISDDLGQIEYIFSDKTGTLTQNLMEFKKCTINGVSYGEAYTEAMAGMQRREGINVEEVSKKAHEAISKSRVTMLQQLRSIHDNPYLHDDRLTFVAPEFVSHLTGTIGEEQKAANEHFMLALALCHTVITERTPGDPPTIEFKAQSPDEAALVATARDCGFTVLGRSADDIRLNVMGEERSYTVLNTLEFNSSRKRMSAIIRMPDGRIVLFCKGADSIIYSRLARGKQQQLRKATAEHLEMFAREGLRTLCIAERVLSEEEYQQWSKEHDLAAQSLTDRDAKLEEVSSAIEQELTLLGGTAIEDRLQDGVPDTISLLASAGIKLWVLTGDKVETAINIGFSCNLLSNEMDLIVFNIDKDDLVSANTELDTNLATFGLTGSDEELLAAQSNHEPPAPTHALIIDGDTLKLMLSPELKQKFLLLCKQCKSVLCCRVSPAQKAAVVQMVKTGLHVMALAIGDGANDVAMIQEADVGVGIAGEEGRQAAMSSDYAIGQFRFLQRLVLVHGRWSYRRLCETIANFFYKNLVWTLALFWYSIYNNFDGSYLFDFTYIVLVNLAFTSLPVILMGILDQDVDDKVSLAVPQLYKTGIEQKEWTQKKFWLYMLDGLYQSIICFFMTYLVYRPATGVTDNGLDLSDRMRMGVFVACSAIIASNTYILLNTYRWDWLTVLINVISSLLIFFWTGVYTSVESSGQFYKAGQEVFGTLSFWALTFLTVTICLAPRFAIKSIQKIYLPRDVDIIREQVILGKFKYLDEKEGFVPPMSRDLSPTSTDLGKPLEVSTKRSKSMPEDERPIYPPSVAPTATTHNPRSQNGSDGTGYTASLDFRHQQQQSVDRGPSYDRVQQSMDPVRGSFEASRDFTSAAMLSRMESTHVDPNE","length":1489,"molWeight":167358,"crc64":"ED5BA1C3B7AE8A0A","md5":"474EA0FF4C5C358BE1F34053E17A1741"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":5,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Transmembrane":9,"Domain":2,"Region":3,"Compositional bias":3,"Active site":1,"Binding site":18},"uniParcId":"UPI000801E280"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7P6V5","uniProtkbId":"A0A1B7P6V5_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2016-11-02","entryVersion":29,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84637.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"NADPH-dependent diflavin oxidoreductase 1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"1.18.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"NADPH-dependent FMN and FAD-containing oxidoreductase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"TAH18"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84637.1"}],"value":"ACJ72_01001"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H(2)O(2)-induced cell death"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP(+) + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:67716"},{"database":"Rhea","id":"RHEA-COMP:17327"},{"database":"Rhea","id":"RHEA-COMP:17328"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:33737"},{"database":"ChEBI","id":"CHEBI:33738"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24040"},{"database":"Rhea","id":"RHEA-COMP:14627"},{"database":"Rhea","id":"RHEA-COMP:14628"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:55376"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:60344"}],"ecNumber":"1.6.2.4","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049342"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"FAD","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001974"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:57692"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"FMN","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001917"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00088"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:58210"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"Relocalizes to mitochondria after H(2)O(2) exposure"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"Mitochondrion","id":"SL-0173"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"value":"In the N-terminal section; belongs to the flavodoxin family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00088"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84637.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"Flavodoxin-like","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50902"}]},{"type":"Domain","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":497,"modifier":"EXACT"}},"description":"FAD-binding FR-type","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51384"}]},{"type":"Binding site","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"FMN","id":"ChEBI:CHEBI:58210"}},{"type":"Binding site","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00088"}],"ligand":{"name":"FMN","id":"ChEBI:CHEBI:58210"}},{"type":"Binding site","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"FMN","id":"ChEBI:CHEBI:58210"}},{"type":"Binding site","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":136,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00088"}],"ligand":{"name":"FMN","id":"ChEBI:CHEBI:58210"}},{"type":"Binding site","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"FMN","id":"ChEBI:CHEBI:58210"}},{"type":"Binding site","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58210"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"FMN","id":"ChEBI:CHEBI:58210"}},{"type":"Binding site","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":418,"modifier":"EXACT"},"end":{"value":421,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":466,"modifier":"EXACT"},"end":{"value":469,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}},{"type":"Binding site","location":{"start":{"value":508,"modifier":"EXACT"},"end":{"value":508,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"NADP(+)","id":"ChEBI:CHEBI:58349"}},{"type":"Binding site","location":{"start":{"value":577,"modifier":"EXACT"},"end":{"value":578,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"NADP(+)","id":"ChEBI:CHEBI:58349"}},{"type":"Binding site","location":{"start":{"value":583,"modifier":"EXACT"},"end":{"value":587,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58349"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"NADP(+)","id":"ChEBI:CHEBI:58349"}},{"type":"Binding site","location":{"start":{"value":668,"modifier":"EXACT"},"end":{"value":668,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57692"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"ligand":{"name":"FAD","id":"ChEBI:CHEBI:57692"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022827"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"id":"KW-0274","category":"Ligand","name":"FAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022630"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022643"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"id":"KW-0288","category":"Ligand","name":"FMN"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_03178"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84637.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84637.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000061","properties":[{"key":"ProteinId","value":"OAX84637.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7P6V5","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7P6V5","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1856718at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0050660","properties":[{"key":"GoTerm","value":"F:flavin adenine dinucleotide binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0010181","properties":[{"key":"GoTerm","value":"F:FMN binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0050661","properties":[{"key":"GoTerm","value":"F:NADP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003958","properties":[{"key":"GoTerm","value":"F:NADPH-hemoprotein reductase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0160246","properties":[{"key":"GoTerm","value":"F:NADPH-iron-sulfur [2Fe-2S] protein oxidoreductase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016226","properties":[{"key":"GoTerm","value":"P:iron-sulfur cluster assembly"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"FunFam","id":"1.20.990.10:FF:000013","properties":[{"key":"EntryName","value":"NADPH-dependent diflavin oxidoreductase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.360:FF:000034","properties":[{"key":"EntryName","value":"NADPH-dependent diflavin oxidoreductase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.80:FF:000030","properties":[{"key":"EntryName","value":"NADPH-dependent diflavin oxidoreductase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.360","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.990.10","properties":[{"key":"EntryName","value":"NADPH-cytochrome p450 Reductase, Chain A, domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.80","properties":[{"key":"EntryName","value":"Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_03178","properties":[{"key":"EntryName","value":"NDOR1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003097","properties":[{"key":"EntryName","value":"CysJ-like_FAD-binding"}]},{"database":"InterPro","id":"IPR017927","properties":[{"key":"EntryName","value":"FAD-bd_FR_type"}]},{"database":"InterPro","id":"IPR001094","properties":[{"key":"EntryName","value":"Flavdoxin-like"}]},{"database":"InterPro","id":"IPR008254","properties":[{"key":"EntryName","value":"Flavodoxin/NO_synth"}]},{"database":"InterPro","id":"IPR001709","properties":[{"key":"EntryName","value":"Flavoprot_Pyr_Nucl_cyt_Rdtase"}]},{"database":"InterPro","id":"IPR029039","properties":[{"key":"EntryName","value":"Flavoprotein-like_sf"}]},{"database":"InterPro","id":"IPR039261","properties":[{"key":"EntryName","value":"FNR_nucleotide-bd"}]},{"database":"InterPro","id":"IPR023173","properties":[{"key":"EntryName","value":"NADPH_Cyt_P450_Rdtase_alpha"}]},{"database":"InterPro","id":"IPR028879","properties":[{"key":"EntryName","value":"NDOR1"}]},{"database":"InterPro","id":"IPR001433","properties":[{"key":"EntryName","value":"OxRdtase_FAD/NAD-bd"}]},{"database":"InterPro","id":"IPR017938","properties":[{"key":"EntryName","value":"Riboflavin_synthase-like_b-brl"}]},{"database":"PANTHER","id":"PTHR19384:SF10","properties":[{"key":"EntryName","value":"NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR19384","properties":[{"key":"EntryName","value":"NITRIC OXIDE SYNTHASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00667","properties":[{"key":"EntryName","value":"FAD_binding_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00258","properties":[{"key":"EntryName","value":"Flavodoxin_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00175","properties":[{"key":"EntryName","value":"NAD_binding_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00369","properties":[{"key":"EntryName","value":"FLAVODOXIN"}]},{"database":"PRINTS","id":"PR00371","properties":[{"key":"EntryName","value":"FPNCR"}]},{"database":"SUPFAM","id":"SSF52343","properties":[{"key":"EntryName","value":"Ferredoxin reductase-like, C-terminal NADP-linked domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52218","properties":[{"key":"EntryName","value":"Flavoproteins"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF63380","properties":[{"key":"EntryName","value":"Riboflavin synthase domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51384","properties":[{"key":"EntryName","value":"FAD_FR"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50902","properties":[{"key":"EntryName","value":"FLAVODOXIN_LIKE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MASANSNKSEQASARAAIILYGSETGNSQDIADELGRLTERLRFSARVCELNAITAESLNEYDVAIFAVSTTGQGDFPANARVFWKSLLLKKLPTTFLHRVKFALFGLGDSSYPKFNWASRKLYKRLLQLGAYEVYPCGEADEQHPEGLEGTFVPWSLDLRKHLLDKFPLAPGQYPIRDDVQLPPKWILAHRDPSLINGTNTYPGVGLATVQSGEQLEDVSIPSHFSHDRRPIPNTVTATLAANNRLTPHTHWQDVRHLILTIPEPIRYVPGDILHITPRNFAVDVNSLISIMGWGNDADKPLCFVPNVEHVSASAASSPEIPFLLNSPGFTLRTLLTDYLDIMAIPRRSFFSNIAHFTNDAIHRDRLLEFTNPEYIDEFYDYTSRPRRSILEVLHEFESVKIPWQLVCTVFPVLRGRQFSLASGGKLKTPESLLTSDAATGAGTRFDLLVAIVKYQTVIKKTREGVCTRYLAVLRPGSTLKVEVQKGGLNSSMRQLLEPTVLIGPGTGVAPLRSMLWEKAAMATTFRQKHGPNVPVPLGPIILLYGGRNRGADFFFEEEWEELKKVLDLTVLTAFSRDQKQKVYVQDRIREHAGMFFKILHELGGTVYICGSSGKMPQAIREALIESFQEFGRCGSDYGEGRGYSREEAEKYLMDMEKVGRYKQETW","length":668,"molWeight":74983,"crc64":"7818296413D13364","md5":"6AEBD076AD2D35512C8B3AE2B19CFA2A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":3,"CAUTION":2},"countByFeatureType":{"Domain":2,"Binding site":13},"uniParcId":"UPI0007FC8A01"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7P7L0","uniProtkbId":"A0A1B7P7L0_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":41,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84983.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00018572"}],"value":"Serine/threonine-protein kinase ATG1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012513"}],"value":"2.7.11.1"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00030237"}],"value":"Autophagy-related protein 1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00019599"}],"value":"Serine/threonine-protein kinase atg1"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84983.1"}],"value":"ACJ72_00637"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17989"},{"database":"Rhea","id":"RHEA-COMP:9863"},{"database":"Rhea","id":"RHEA-COMP:11604"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29999"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:83421"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048679"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10027"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:46608"},{"database":"Rhea","id":"RHEA-COMP:11060"},{"database":"Rhea","id":"RHEA-COMP:11605"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30013"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:61977"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047899"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10027"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011138"}],"value":"Homodimer. Forms a ternary complex with ATG13 and ATG17"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004623"}],"value":"Preautophagosomal structure membrane","id":"SL-0221"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004623"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00060750"}],"value":"Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84983.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":337,"modifier":"EXACT"}},"description":"Protein kinase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50011"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":354,"modifier":"EXACT"},"end":{"value":502,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":558,"modifier":"EXACT"},"end":{"value":607,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":712,"modifier":"EXACT"},"end":{"value":732,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":780,"modifier":"EXACT"},"end":{"value":800,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":924,"modifier":"EXACT"},"end":{"value":945,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":357,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":436,"modifier":"EXACT"},"end":{"value":447,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":493,"modifier":"EXACT"},"end":{"value":502,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":562,"modifier":"EXACT"},"end":{"value":574,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":583,"modifier":"EXACT"},"end":{"value":599,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":782,"modifier":"EXACT"},"end":{"value":800,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":924,"modifier":"EXACT"},"end":{"value":934,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10027"}]},{"type":"Binding site","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10141"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10141"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023006"}],"id":"KW-0072","category":"Biological process","name":"Autophagy"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022777"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10141"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022927"}],"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022527"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"id":"KW-0723","category":"Molecular function","name":"Serine/threonine-protein kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84983.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84983.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000035","properties":[{"key":"ProteinId","value":"OAX84983.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7P7L0","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7P7L0","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"346907at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005776","properties":[{"key":"GoTerm","value":"C:autophagosome"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0034045","properties":[{"key":"GoTerm","value":"C:phagophore assembly site membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004674","properties":[{"key":"GoTerm","value":"F:protein serine/threonine kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000045","properties":[{"key":"GoTerm","value":"P:autophagosome assembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0000422","properties":[{"key":"GoTerm","value":"P:autophagy of mitochondrion"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0034727","properties":[{"key":"GoTerm","value":"P:piecemeal microautophagy of the nucleus"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0010506","properties":[{"key":"GoTerm","value":"P:regulation of autophagy"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0042594","properties":[{"key":"GoTerm","value":"P:response to starvation"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0061709","properties":[{"key":"GoTerm","value":"P:reticulophagy"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd14009","properties":[{"key":"EntryName","value":"STKc_ATG1_ULK_like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.510.10:FF:000817","properties":[{"key":"EntryName","value":"Serine/threonine-protein kinase ATG1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.200.20","properties":[{"key":"EntryName","value":"Phosphorylase Kinase, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.510.10","properties":[{"key":"EntryName","value":"Transferase(Phosphotransferase) domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR045269","properties":[{"key":"EntryName","value":"Atg1-like"}]},{"database":"InterPro","id":"IPR048941","properties":[{"key":"EntryName","value":"ATG1-like_MIT2"}]},{"database":"InterPro","id":"IPR022708","properties":[{"key":"EntryName","value":"Atg1-like_tMIT"}]},{"database":"InterPro","id":"IPR011009","properties":[{"key":"EntryName","value":"Kinase-like_dom_sf"}]},{"database":"InterPro","id":"IPR000719","properties":[{"key":"EntryName","value":"Prot_kinase_dom"}]},{"database":"InterPro","id":"IPR017441","properties":[{"key":"EntryName","value":"Protein_kinase_ATP_BS"}]},{"database":"InterPro","id":"IPR008271","properties":[{"key":"EntryName","value":"Ser/Thr_kinase_AS"}]},{"database":"PANTHER","id":"PTHR24348:SF22","properties":[{"key":"EntryName","value":"NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR24348","properties":[{"key":"EntryName","value":"SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12063","properties":[{"key":"EntryName","value":"ATG1-like_MIT1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21127","properties":[{"key":"EntryName","value":"ATG1-like_MIT2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00069","properties":[{"key":"EntryName","value":"Pkinase"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00220","properties":[{"key":"EntryName","value":"S_TKc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56112","properties":[{"key":"EntryName","value":"Protein kinase-like (PK-like)"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00107","properties":[{"key":"EntryName","value":"PROTEIN_KINASE_ATP"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50011","properties":[{"key":"EntryName","value":"PROTEIN_KINASE_DOM"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00108","properties":[{"key":"EntryName","value":"PROTEIN_KINASE_ST"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDSPKDATPATRPPPSRRRREMDVVPIQVGSYTRLVEIGRGSFATVYQGVHNRNQSLVAIKSVNMARLNKKLKDNLKLEINILKAVHHPHIVMLLDCQETSNEIHLVMEYCVLGDLSIFIKKRDSLVRHQLTRDLILRYPNPQSGGLNEVIVRHFLKQLASALHFLRSRDLVHRDVKPQNLLLNPSPQSIARGDYRIPPYKGNEDLFTPLAGLDSMPLLKLADFGFARSLPSTSLADTLCGSPLYMAPEILRYEKYDAKADLWSVGTVLYEMVVGKPYFRATNHVELLHKIEKANDRIKFPEHCEASETMKKTIRHLLKRNPVERMGFHDFFESPVIKGDIPGLVNEDRYPTAQEKSIADDVRKAGSPRQDRDVSAAQEAGYSSSPPDRRSLGQAAEGPRPTTPRPVSGSSPRPAAAVRRIGSRDKPDLQRAAVTGRQQNLNSQPSSPGHPEPGEQTAAAHAMERQRSRNTSSPGSSLFKETAVERGTPPKPTFDDKVAERERERAAQEVAFERDYIIVEKRAVEVNAFADELAASPRIQQQNQGSARPATGTIIRRATTTSHSPSTSQGTPTRALQMVTGRPRADSSHNRHNSYDRRYGPSPTSATSAISKALNMASGRLFGVGFSPPLTVTKGGRSPPLAYNPFPAYPLSHHGNLVVIGDGARTQVALDEDSKTVQIIEECATRSDVVYGFAEVKYKQLIPLAPSMQSEAAIHPSAASPDMDDPSQSTDGGLTVDAVVTLSEEALVLYVKALSLLAKSMDIAGAWWARKNTGEVIGIGDSSSSSSGNNNGSGNTTPAVGNRVNNVVQWVRNRFNEVLEKAEFVRLKLVESQKRLPTDHPSHPSNNSNYSYGSDAGLGLGTSVDQIVVSSGTTAEKLMYDRALEMSRTAAINELTGEDLPGCEIAYVTAIRMLEAVLEDDEEALSRRSGHGERTGVASGDSDRDVINGVQVEDRQIVAKRESCYIFHSVLQKK","length":974,"molWeight":106873,"crc64":"95AAA70DDFD67EB4","md5":"F8D41DA71CA40BF97119DC2B6CCC4A4B"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Region":6,"Compositional bias":7,"Active site":1,"Binding site":1},"uniParcId":"UPI0008010AEA"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7P7W3","uniProtkbId":"A0A1B7P7W3_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":42,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84987.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00018572"}],"value":"Serine/threonine-protein kinase ATG1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012513"}],"value":"2.7.11.1"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00030237"}],"value":"Autophagy-related protein 1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00019599"}],"value":"Serine/threonine-protein kinase atg1"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84987.1"}],"value":"ACJ72_00641"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17989"},{"database":"Rhea","id":"RHEA-COMP:9863"},{"database":"Rhea","id":"RHEA-COMP:11604"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29999"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:83421"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048679"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10027"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:46608"},{"database":"Rhea","id":"RHEA-COMP:11060"},{"database":"Rhea","id":"RHEA-COMP:11605"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30013"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:61977"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047899"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10027"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011138"}],"value":"Homodimer. Forms a ternary complex with ATG13 and ATG17"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004623"}],"value":"Preautophagosomal structure membrane","id":"SL-0221"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004623"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00005354"}],"value":"Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"value":"Belongs to the protein kinase superfamily. Ser/Thr protein kinase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84987.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"Protein kinase","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50011"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":324,"modifier":"EXACT"},"end":{"value":459,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":515,"modifier":"EXACT"},"end":{"value":564,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":669,"modifier":"EXACT"},"end":{"value":689,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":737,"modifier":"EXACT"},"end":{"value":757,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":881,"modifier":"EXACT"},"end":{"value":902,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":327,"modifier":"EXACT"},"end":{"value":344,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":406,"modifier":"EXACT"},"end":{"value":417,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":432,"modifier":"EXACT"},"end":{"value":441,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":450,"modifier":"EXACT"},"end":{"value":459,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":519,"modifier":"EXACT"},"end":{"value":531,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":540,"modifier":"EXACT"},"end":{"value":556,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":739,"modifier":"EXACT"},"end":{"value":757,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":881,"modifier":"EXACT"},"end":{"value":891,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10027"}]},{"type":"Binding site","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10141"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10141"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023006"}],"id":"KW-0072","category":"Biological process","name":"Autophagy"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022777"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10141"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022927"}],"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022527"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"id":"KW-0723","category":"Molecular function","name":"Serine/threonine-protein kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00159"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84987.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX84987.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01000035","properties":[{"key":"ProteinId","value":"OAX84987.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7P7W3","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7P7W3","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"346907at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005776","properties":[{"key":"GoTerm","value":"C:autophagosome"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0034045","properties":[{"key":"GoTerm","value":"C:phagophore assembly site membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0004674","properties":[{"key":"GoTerm","value":"F:protein serine/threonine kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000045","properties":[{"key":"GoTerm","value":"P:autophagosome assembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0000422","properties":[{"key":"GoTerm","value":"P:autophagy of mitochondrion"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0034727","properties":[{"key":"GoTerm","value":"P:piecemeal microautophagy of the nucleus"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0010506","properties":[{"key":"GoTerm","value":"P:regulation of autophagy"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0042594","properties":[{"key":"GoTerm","value":"P:response to starvation"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0061709","properties":[{"key":"GoTerm","value":"P:reticulophagy"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"FunFam","id":"3.30.200.20:FF:000042","properties":[{"key":"EntryName","value":"Aurora kinase A"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.510.10","properties":[{"key":"EntryName","value":"Transferase(Phosphotransferase) domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR045269","properties":[{"key":"EntryName","value":"Atg1-like"}]},{"database":"InterPro","id":"IPR048941","properties":[{"key":"EntryName","value":"ATG1-like_MIT2"}]},{"database":"InterPro","id":"IPR022708","properties":[{"key":"EntryName","value":"Atg1-like_tMIT"}]},{"database":"InterPro","id":"IPR011009","properties":[{"key":"EntryName","value":"Kinase-like_dom_sf"}]},{"database":"InterPro","id":"IPR000719","properties":[{"key":"EntryName","value":"Prot_kinase_dom"}]},{"database":"InterPro","id":"IPR017441","properties":[{"key":"EntryName","value":"Protein_kinase_ATP_BS"}]},{"database":"InterPro","id":"IPR008271","properties":[{"key":"EntryName","value":"Ser/Thr_kinase_AS"}]},{"database":"PANTHER","id":"PTHR24348:SF22","properties":[{"key":"EntryName","value":"NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR24348","properties":[{"key":"EntryName","value":"SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12063","properties":[{"key":"EntryName","value":"ATG1-like_MIT1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21127","properties":[{"key":"EntryName","value":"ATG1-like_MIT2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00069","properties":[{"key":"EntryName","value":"Pkinase"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00220","properties":[{"key":"EntryName","value":"S_TKc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56112","properties":[{"key":"EntryName","value":"Protein kinase-like (PK-like)"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00107","properties":[{"key":"EntryName","value":"PROTEIN_KINASE_ATP"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50011","properties":[{"key":"EntryName","value":"PROTEIN_KINASE_DOM"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00108","properties":[{"key":"EntryName","value":"PROTEIN_KINASE_ST"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDSPKDATPATRPPPSRRRREMDVVPIQVGSYTRLVEIGRGSFATVYQGVHNRNQSLVAIKSVNMARLNKKLKDNLKLEINILKAVHHPHIVMLLDCQETSNEIHLVMEYCVLGDLSIFIKKRDSLVRHQLTRDLILRYPNPQSGGLNEVIVRHFLKQLASALHFLRSRDLVHRDVKPQNLLLNPSPQSIARGDYRIPPYKGNEDLFTPLAGLDSMPLLKLADFGFARSLPSTSLADTLCGSPLYMAPEILRYEKYDAKADLWSVGTVLYEMVVGKPYFRATNHVELLHKIEKANDRIKFPEHCEAIKGDIPGLVNEDRYPTAQEKSIADDVRKAGSPRQDRDVSAAQEAGYSSSPPDRRSLGQAAEGPRPTTPRPVSGSSPRPAAAVRRIGSRDKPDLQRAAVTGRQQNLNSQPSSPGHPEPGEQTAAAHAMERQRRTAVERGTPPKPTFDDKVAERERERAAQEVAFERDYIIVEKRAVEVNAFADELAASPRIQQQNQGSARPATGTIIRRATTTSHSPSTSQGTPTRALQMVTGRPRADSSHNRHNSYDRRYGPSPTSATSAISKALNMASGRLFGVGFSPPLTVTKGGRSPPLAYNPFPAYPLSHHGNLVVIGDGARTQVALDEDSKTVQIIEECATRSDVVYGFAEVKYKQLIPLAPSMQSEAAIHPSAASPDMDDPSQSTDGGLTVDAVVTLSEEALVLYVKALSLLAKSMDIAGAWWARKNTGEVIGIGDSSSSSSGNNNGSGNTTPAVGNRVNNVVQWVRNRFNEVLEKAEFVRLKLVESQKRLPTDHPSHPSNNSNYSYGSDAGLGLGTSVDQIVVSSGTTAEKLMYDRALEMSRTAAINELTGEDLPGCEIAYVTAIRMLEAVLEDDEEALSRRSGHGERTGVASGDSDRDVINGVQVEDRQIVAKHAVGWLGVHMHRSRQPKIHLLRKRKSRVYYNLCEMNSNG","length":956,"molWeight":104873,"crc64":"0EC1EDEA6D8E6F9D","md5":"D7974B9DCE00622630D5E659564C1399"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":2,"CAUTION":1},"countByFeatureType":{"Domain":1,"Region":6,"Compositional bias":8,"Active site":1,"Binding site":1},"uniParcId":"UPI00080254C2"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NJU4","uniProtkbId":"A0A1B7NJU4_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":42,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77002.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}],"value":"Glyceraldehyde-3-phosphate dehydrogenase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}],"value":"1.2.1.12"}]}},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77002.1"}],"value":"ACJ72_08704"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10300"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57604"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:59776"}],"ecNumber":"1.2.1.12","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10009"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004869"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}],"value":"Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011881"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007406"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000397"}],"value":"Belongs to the glyceraldehyde-3-phosphate dehydrogenase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77002.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding","evidences":[{"evidenceCode":"ECO:0000259","source":"SMART","id":"SM00846"}]},{"type":"Active site","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-1"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10009"}]},{"type":"Binding site","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":106,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-2"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-2"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-2"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":189,"modifier":"EXACT"},"end":{"value":189,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-2"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":271,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Site","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"Activates thiol group during catalysis","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-4"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023152"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}],"id":"KW-0324","category":"Biological process","name":"Glycolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77002.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77002.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01003701","properties":[{"key":"ProteinId","value":"OAX77002.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NJU4","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NJU4","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1152826at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00109","properties":[{"key":"RectionId","value":"UER00184"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004365","properties":[{"key":"GoTerm","value":"F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0051287","properties":[{"key":"GoTerm","value":"F:NAD binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0050661","properties":[{"key":"GoTerm","value":"F:NADP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006006","properties":[{"key":"GoTerm","value":"P:glucose metabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"CDD","id":"cd18126","properties":[{"key":"EntryName","value":"GAPDH_I_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd05214","properties":[{"key":"EntryName","value":"GAPDH_I_N"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.360.10:FF:000001","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.720:FF:000001","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.360.10","properties":[{"key":"EntryName","value":"Dihydrodipicolinate Reductase, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR020831","properties":[{"key":"EntryName","value":"GlycerAld/Erythrose_P_DH"}]},{"database":"InterPro","id":"IPR020830","properties":[{"key":"EntryName","value":"GlycerAld_3-P_DH_AS"}]},{"database":"InterPro","id":"IPR020829","properties":[{"key":"EntryName","value":"GlycerAld_3-P_DH_cat"}]},{"database":"InterPro","id":"IPR020828","properties":[{"key":"EntryName","value":"GlycerAld_3-P_DH_NAD(P)-bd"}]},{"database":"InterPro","id":"IPR006424","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-P_DH_1"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"NCBIfam","id":"TIGR01534","properties":[{"key":"EntryName","value":"GAPDH-I"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10836","properties":[{"key":"EntryName","value":"GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10836:SF76","properties":[{"key":"EntryName","value":"GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02800","properties":[{"key":"EntryName","value":"Gp_dh_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00044","properties":[{"key":"EntryName","value":"Gp_dh_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000149","properties":[{"key":"EntryName","value":"GAP_DH"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00078","properties":[{"key":"EntryName","value":"G3PDHDRGNASE"}]},{"database":"SMART","id":"SM00846","properties":[{"key":"EntryName","value":"Gp_dh_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55347","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00071","properties":[{"key":"EntryName","value":"GAPDH"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLKYDSTHGQFKGDIQVSENGLTVNGKTVHFYQERDPANIPWAKHGAHYVVESTGVFTTTEKASAHLKGGAKKVIISAPSADAPMFVMGVNEKTYKPDINVLSNASCTTNCLAPLAKVIHDNFGLTEGLMTTIHSYTATQKTVDGPSSKDWRGGRTAAQNIIPSSTGAAKAVGKVIPALNGKLTGMSMRVPTSNVSVVDLTCKTEKAVTYDQIKKTIKEASEGELKGILGYSEDALVSTDLNGDPRSSIFDASAGIALNDHFIKLISWYDNEWGYSRRVLDLISYIAKVDAGK","length":293,"molWeight":31503,"crc64":"513D7C6A45E18B55","md5":"EEAB487366635CB08F85A18DA501FD2B"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Active site":1,"Binding site":7,"Site":1},"uniParcId":"UPI000801CB86"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NJY2","uniProtkbId":"A0A1B7NJY2_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2016-11-02","entryVersion":27,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77108.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00067464"}],"value":"Vacuolar transporter chaperone complex subunit 4"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012960"}],"value":"2.7.4.1"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00080494"}],"value":"Polyphosphate kinase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00075894"}],"value":"SPX-dependent polyphosphate polymerase VTC subunit 4"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00081313"}],"value":"Vacuolar membrane polyphosphate polymerase catalytic subunit"}}],"flag":"Fragment"},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77108.1"}],"value":"ACJ72_08597"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"[phosphate](n) + ATP = [phosphate](n+1) + ADP","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19573"},{"database":"Rhea","id":"RHEA-COMP:9859"},{"database":"Rhea","id":"RHEA-COMP:14280"},{"database":"ChEBI","id":"CHEBI:16838"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.4.1","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00050204"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:19574"},"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00050204"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001936"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004128"}],"value":"Vacuole membrane","id":"SL-0271"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004128"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00061390"}],"value":"Belongs to the VTC4 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77108.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"VTC","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF09359"}]},{"type":"Region","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":381,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":290,"modifier":"EXACT"},"end":{"value":299,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":300,"modifier":"EXACT"},"end":{"value":318,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":328,"modifier":"EXACT"},"end":{"value":344,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":354,"modifier":"EXACT"},"end":{"value":368,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Non-terminal residue","location":{"start":{"value":489,"modifier":"EXACT"},"end":{"value":489,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77108.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022554"}],"id":"KW-0926","category":"Cellular component","name":"Vacuole"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77108.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77108.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01003360","properties":[{"key":"ProteinId","value":"OAX77108.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NJY2","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NJY2","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"6493944at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0000329","properties":[{"key":"GoTerm","value":"C:fungal-type vacuole membrane"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0033254","properties":[{"key":"GoTerm","value":"C:vacuolar transporter chaperone complex"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0008976","properties":[{"key":"GoTerm","value":"F:polyphosphate kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0016237","properties":[{"key":"GoTerm","value":"P:microautophagy"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006799","properties":[{"key":"GoTerm","value":"P:polyphosphate biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0007034","properties":[{"key":"GoTerm","value":"P:vacuolar transport"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0042144","properties":[{"key":"GoTerm","value":"P:vacuole fusion, non-autophagic"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd07751","properties":[{"key":"EntryName","value":"PolyPPase_VTC4_like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.100.30:FF:000001","properties":[{"key":"EntryName","value":"Vacuolar transporter chaperone 4"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.100.30","properties":[{"key":"EntryName","value":"VTC, catalytic tunnel domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR051572","properties":[{"key":"EntryName","value":"VTC_Complex_Subunit"}]},{"database":"InterPro","id":"IPR018966","properties":[{"key":"EntryName","value":"VTC_domain"}]},{"database":"InterPro","id":"IPR042267","properties":[{"key":"EntryName","value":"VTC_sf"}]},{"database":"PANTHER","id":"PTHR46140","properties":[{"key":"EntryName","value":"VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46140:SF1","properties":[{"key":"EntryName","value":"VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09359","properties":[{"key":"EntryName","value":"VTC"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKGITFLHLPVLVFNPRKEFDERDSAISSIYYDNPNTWELYTGRLKKTEGAEAIRLRWYGGMENDQIFVERKTHREDWTGEKSVKARFPIKEKYVNAFMSGAMTVESIFEKARKDGKKSEQQIADWEQLAREVQYRVITRKLEPVTRSFYHRTAFQLPGDARVRISLDTELTLIREDNMDGRRRSGNNWRRMDIGIDYPFLQLPAQDVERFPYAILEVKLQTQAGQSSPHWIRELISSHLVEAVPKFSKFIHGTANLFPDQIHLLPFWMPQMDVDIRKPITRGFGIERPVQTSQSTSENPLDDDDDDDDDESDEDGDGDGDKRDGRRPSSSTLHETNGIIFQSHQPHRQGEEGGGGDDDDDGDDDYDPSQDPNIPEATGNALDIEERIAAQRRLMDIGGDDYPLYDSEDESSGQDDLEEAKRIGGWHYRAKLAQHYMRAAGRSTLTAMKFVAPVPRPTAMPTNNGVGGVGRFIPPDQMQVKRFKAPKGK","length":489,"molWeight":55837,"crc64":"BE44272E58FE18D7","md5":"4B52A07561A12CD8013AFA1B869F0937"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Region":1,"Compositional bias":4,"Non-terminal residue":1},"uniParcId":"UPI000801FF9D"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NK89","uniProtkbId":"A0A1B7NK89_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":34,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77096.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362007"}],"value":"Phosphotransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362007"}],"value":"2.7.1.-"}]},"flag":"Fragment"},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77096.1"}],"value":"ACJ72_08609"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22740"},{"database":"ChEBI","id":"CHEBI:4194"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:229467"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.1.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01084"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009225"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01084"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362007"}],"value":"Belongs to the hexokinase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01084"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77096.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":277,"modifier":"EXACT"}},"description":"Hexokinase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00349"}]},{"type":"Domain","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":460,"modifier":"EXACT"}},"description":"Hexokinase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF03727"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":114,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Non-terminal residue","location":{"start":{"value":461,"modifier":"EXACT"},"end":{"value":461,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77096.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01084"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362007"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01084"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362007"}],"id":"KW-0324","category":"Biological process","name":"Glycolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01084"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362007"}],"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01084"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362007"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01084"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU362007"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77096.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77096.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01003400","properties":[{"key":"ProteinId","value":"OAX77096.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NK89","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NK89","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"419537at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00109","properties":[{"key":"RectionId","value":"UER00180"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005536","properties":[{"key":"GoTerm","value":"F:D-glucose binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008865","properties":[{"key":"GoTerm","value":"F:fructokinase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004340","properties":[{"key":"GoTerm","value":"F:glucokinase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0019158","properties":[{"key":"GoTerm","value":"F:mannokinase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0046835","properties":[{"key":"GoTerm","value":"P:carbohydrate phosphorylation"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006006","properties":[{"key":"GoTerm","value":"P:glucose metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0001678","properties":[{"key":"GoTerm","value":"P:intracellular glucose homeostasis"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006013","properties":[{"key":"GoTerm","value":"P:mannose metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd24000","properties":[{"key":"EntryName","value":"ASKHA_NBD_HK"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.420.40:FF:000211","properties":[{"key":"EntryName","value":"Phosphotransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.420.40","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.367.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR043129","properties":[{"key":"EntryName","value":"ATPase_NBD"}]},{"database":"InterPro","id":"IPR001312","properties":[{"key":"EntryName","value":"Hexokinase"}]},{"database":"InterPro","id":"IPR022673","properties":[{"key":"EntryName","value":"Hexokinase_C"}]},{"database":"InterPro","id":"IPR022672","properties":[{"key":"EntryName","value":"Hexokinase_N"}]},{"database":"PANTHER","id":"PTHR19443","properties":[{"key":"EntryName","value":"HEXOKINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR19443:SF29","properties":[{"key":"EntryName","value":"PHOSPHOTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00349","properties":[{"key":"EntryName","value":"Hexokinase_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03727","properties":[{"key":"EntryName","value":"Hexokinase_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00475","properties":[{"key":"EntryName","value":"HEXOKINASE"}]},{"database":"SUPFAM","id":"SSF53067","properties":[{"key":"EntryName","value":"Actin-like ATPase domain"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51748","properties":[{"key":"EntryName","value":"HEXOKINASE_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSQTSAMDEDYPSLDHESYDGNYTDHGMDNGQQNSDDTIDKFLRPLVVSDSTLHRLACRFRHVYRDLALTSDQQFLPTPVTRLPTGQETGRYLAIDVGGTNLRVAFIELLGESKDENGTSTGSPTSRDTMRRAQRQRVNRTLERLWPIGEHLKKDQADDLFLWIGDCMAEVVAESLAPNLANGTPIPEELEMGITFSFPMMQESLSEARLMPMGKGFAIPSNLDLGGILLAGYDRHTRRPLENGQQSAKRQKRFALPKLKIAAITNDTVATLASLAYLVKSLPNSRVAMGIIVGTGCNATIPMSLKDLHESKAKHVSSRHPDAVETIINTEWTINGAAPPLHELNITTTWDTMLDQACARPGFQPFEYMTGGRYIGELVRIIFYDYLTSVVGLSKRELPANLIQEYALTTAYLSSSVAQIQSDPELVQHLTNTLPPPESSDWGWDTNSARSFRKVAQAVQT","length":461,"molWeight":51159,"crc64":"E0BABDBD229093AD","md5":"BFE0C565B4EBB0F7CC7585E2C66D56C9"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"SIMILARITY":1,"CAUTION":2},"countByFeatureType":{"Domain":2,"Region":2,"Compositional bias":1,"Non-terminal residue":1},"uniParcId":"UPI0007FED19F"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NKF8","uniProtkbId":"A0A1B7NKF8_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2016-11-02","entryVersion":27,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77265.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"value":"Adenylosuccinate synthetase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"value":"6.3.4.4"}]},"flag":"Fragment"},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77265.1"}],"value":"ACJ72_08439"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003779"}],"value":"Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"value":"Plays an important role in the de novo pathway of purine nucleotide biosynthesis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15753"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29991"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57567"},{"database":"ChEBI","id":"CHEBI:58053"},{"database":"ChEBI","id":"CHEBI:58189"}],"ecNumber":"6.3.4.4","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"value":"Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011738"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"value":"Belongs to the adenylosuccinate synthetase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77265.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Active site","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10134"}]},{"type":"Non-terminal residue","location":{"start":{"value":187,"modifier":"EXACT"},"end":{"value":187,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77265.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023134"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022598"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022755"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000520"}],"id":"KW-0658","category":"Biological process","name":"Purine biosynthesis"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77265.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77265.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01002934","properties":[{"key":"ProteinId","value":"OAX77265.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NKF8","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NKF8","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"10265645at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00075","properties":[{"key":"RectionId","value":"UER00335"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004019","properties":[{"key":"GoTerm","value":"F:adenylosuccinate synthase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0044208","properties":[{"key":"GoTerm","value":"P:'de novo' AMP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"GO","id":"GO:0046040","properties":[{"key":"GoTerm","value":"P:IMP metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"FunFam","id":"1.10.300.10:FF:000001","properties":[{"key":"EntryName","value":"Adenylosuccinate synthetase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.440.10","properties":[{"key":"EntryName","value":"Adenylosuccinate Synthetase, subunit A, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.300.10","properties":[{"key":"EntryName","value":"Adenylosuccinate Synthetase, subunit A, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00011","properties":[{"key":"EntryName","value":"Adenylosucc_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR018220","properties":[{"key":"EntryName","value":"Adenylosuccin_syn_GTP-bd"}]},{"database":"InterPro","id":"IPR033128","properties":[{"key":"EntryName","value":"Adenylosuccin_syn_Lys_AS"}]},{"database":"InterPro","id":"IPR042109","properties":[{"key":"EntryName","value":"Adenylosuccinate_synth_dom1"}]},{"database":"InterPro","id":"IPR042110","properties":[{"key":"EntryName","value":"Adenylosuccinate_synth_dom2"}]},{"database":"InterPro","id":"IPR001114","properties":[{"key":"EntryName","value":"Adenylosuccinate_synthetase"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"PANTHER","id":"PTHR11846","properties":[{"key":"EntryName","value":"ADENYLOSUCCINATE SYNTHETASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11846:SF0","properties":[{"key":"EntryName","value":"ADENYLOSUCCINATE SYNTHETASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00709","properties":[{"key":"EntryName","value":"Adenylsucc_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00788","properties":[{"key":"EntryName","value":"Adenylsucc_synt"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01266","properties":[{"key":"EntryName","value":"ADENYLOSUCCIN_SYN_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00513","properties":[{"key":"EntryName","value":"ADENYLOSUCCIN_SYN_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVTIILGAQFGDEGKGKITDILSQSATLCCRAAGGHNAGHTIVHDNITYDFHILPSGLISPDCINLIGTGTVVHVPSFFKELAALQNKGLKDADKRIFVSDRAQVCFDLHAVVDGLEEAILAGKKVGTTGKGIGPCYSDKAARRGVRIGEVLEEGVVESKLRSLEAGYRKRFGELPYDLEEEVKRFN","length":187,"molWeight":20184,"crc64":"623A7B360D5476E4","md5":"06B3DA5C89F3878622285941A7B18B4B"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Active site":1,"Non-terminal residue":1},"uniParcId":"UPI0007FC30B9"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NKI6","uniProtkbId":"A0A1B7NKI6_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2016-11-02","entryVersion":36,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77295.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012119"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"value":"Adenosine kinase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"value":"AK"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012119"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"value":"2.7.1.20"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"value":"Adenosine 5'-phosphotransferase"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77295.1"}],"value":"ACJ72_08409"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"value":"ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"adenosine + ATP = AMP + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20824"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16335"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:456215"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.1.20","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004801"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"value":"Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010688"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"value":"Belongs to the carbohydrate kinase PfkB family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77295.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":312,"modifier":"EXACT"}},"description":"Carbohydrate kinase PfkB","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00294"}]},{"type":"Active site","location":{"start":{"value":276,"modifier":"EXACT"},"end":{"value":276,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR601805-1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022777"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022726"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"id":"KW-0660","category":"Biological process","name":"Purine salvage"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU368116"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77295.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77295.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01002878","properties":[{"key":"ProteinId","value":"OAX77295.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NKI6","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NKI6","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"432447at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00588","properties":[{"key":"RectionId","value":"UER00659"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004001","properties":[{"key":"GoTerm","value":"F:adenosine kinase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0044209","properties":[{"key":"GoTerm","value":"P:AMP salvage"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006144","properties":[{"key":"GoTerm","value":"P:purine nucleobase metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006166","properties":[{"key":"GoTerm","value":"P:purine ribonucleoside salvage"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd01168","properties":[{"key":"EntryName","value":"adenosine_kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.1190.20:FF:000014","properties":[{"key":"EntryName","value":"ADO1p Adenosine kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1110.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1190.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001805","properties":[{"key":"EntryName","value":"Adenokinase"}]},{"database":"InterPro","id":"IPR011611","properties":[{"key":"EntryName","value":"PfkB_dom"}]},{"database":"InterPro","id":"IPR029056","properties":[{"key":"EntryName","value":"Ribokinase-like"}]},{"database":"PANTHER","id":"PTHR45769","properties":[{"key":"EntryName","value":"ADENOSINE KINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45769:SF3","properties":[{"key":"EntryName","value":"ADENOSINE KINASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00294","properties":[{"key":"EntryName","value":"PfkB"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00989","properties":[{"key":"EntryName","value":"ADENOKINASE"}]},{"database":"SUPFAM","id":"SSF53613","properties":[{"key":"EntryName","value":"Ribokinase-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLEKYGLKANDAILAEEKHMGLYEDLIQNHNAKLIAGGAAQNTARGAQYILPPNSVLYIGCVGKDKYADILQEACNKAGLRTEYRIDESQPTGRCGVIITGHERSLCTHLAASNEYKLDHLKQPHIWSLVEKAKVYYVGGYHLTVCVPAILALAEEAAEKNKTFMLSLSAPFIPQFFKDQLDSVIPYTDYVLGNEAEALSYSESHGWGITDIQEIAKKMATLPKKNTKRPRIVIITQGTEPTISAIASADGLDEVRLTSVHAISKDEINDTNGAGDAFAGGFCAGVVEGKTVAESIDMGHWLAGLSIRELGPS","length":313,"molWeight":33963,"crc64":"FB1E004018953708","md5":"EFB257FD5AF492D52559469D05C22516"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Active site":1},"uniParcId":"UPI0007FF96D0"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NKI7","uniProtkbId":"A0A1B7NKI7_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2025-06-18","lastSequenceUpdateDate":"2016-11-02","entryVersion":30,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77333.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"value":"E3 ubiquitin-protein ligase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"value":"2.3.2.27"}]},"flag":"Fragment"},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77333.1"}],"value":"ACJ72_08371"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"value":"Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.","ecNumber":"2.3.2.27","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000900"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"value":"Protein modification; protein ubiquitination"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00046341"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"value":"Belongs to the E3 ubiquitin-protein ligase UBR1-like family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77333.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"UBR-type","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51157"}]},{"type":"Zinc finger","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"UBR-type","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00508"}]},{"type":"Region","location":{"start":{"value":391,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":394,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Non-terminal residue","location":{"start":{"value":433,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77333.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022786"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"id":"KW-0833","category":"Biological process","name":"Ubl conjugation pathway"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022833"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"id":"KW-0862","category":"Ligand","name":"Zinc"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022771"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU366018"}],"id":"KW-0863","category":"Domain","name":"Zinc-finger"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77333.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77333.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01002794","properties":[{"key":"ProteinId","value":"OAX77333.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NKI7","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NKI7","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"26387at2759","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00143","properties":[{"key":"RectionId","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0000151","properties":[{"key":"GoTerm","value":"C:ubiquitin ligase complex"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0061630","properties":[{"key":"GoTerm","value":"F:ubiquitin protein ligase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008270","properties":[{"key":"GoTerm","value":"F:zinc ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016567","properties":[{"key":"GoTerm","value":"P:protein ubiquitination"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0071596","properties":[{"key":"GoTerm","value":"P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd19673","properties":[{"key":"EntryName","value":"UBR-box_UBR3"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.10.110.30:FF:000001","properties":[{"key":"EntryName","value":"E3 ubiquitin-protein ligase UBR2 isoform 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.10.110.30","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003769","properties":[{"key":"EntryName","value":"ClpS_core"}]},{"database":"InterPro","id":"IPR039164","properties":[{"key":"EntryName","value":"UBR1-like"}]},{"database":"InterPro","id":"IPR003126","properties":[{"key":"EntryName","value":"Znf_UBR"}]},{"database":"PANTHER","id":"PTHR21497:SF24","properties":[{"key":"EntryName","value":"E3 UBIQUITIN-PROTEIN LIGASE UBR1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21497","properties":[{"key":"EntryName","value":"UBIQUITIN LIGASE E3 ALPHA-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02617","properties":[{"key":"EntryName","value":"ClpS"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02207","properties":[{"key":"EntryName","value":"zf-UBR"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00396","properties":[{"key":"EntryName","value":"ZnF_UBR1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51157","properties":[{"key":"EntryName","value":"ZF_UBR"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLSDFERKLGRALLEHPSRHGYRYTKEASQDLLELLFRSMTGYNEEYLQQMEVGGSQGAVEGAEYTEAARGKRCGHIFKNGEASYHCMTCSTDDTCALCTRCFDSSDHTGHKYSISLSSGYNGCCDCGDEEAFKIPVLCAIHTATRDAEGKGKSSSSSSSSAAQTPADLVESVTTTISRVYDYFCDVISCSPEQLRLAKSESSIRQDEAASRLSAEWYGGSDPVEEEPEFALVLWNDEKHTVTEVANQVVRACREVARFGKEKANETHEIGRSVVKYSRDLQSLLAVSNIIEQIKVTVTVRSARDTYREQMCGTIIEWLADISSCSIGDDNDLLRQTVCDQMIQPWRLGSSASNADIGKKGIYDNEEEDRLRDYSVRVSIGPDGPFIEVNATVDEMDDNDEGAMEEEHEEDGGEEDENRIDDDEDIDMDEVHR","length":433,"molWeight":48217,"crc64":"75D34F1E8E61CAC9","md5":"2B82CC81F7101755157E88218DA9167C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":1,"Zinc finger":1,"Region":1,"Compositional bias":1,"Non-terminal residue":1},"uniParcId":"UPI0007FEEC6F"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A1B7NKJ6","uniProtkbId":"A0A1B7NKJ6_9EURO","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2016-11-02","entryVersion":28,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Emergomyces africanus","taxonId":1955775,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77359.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Ajellomycetaceae","Emergomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00070480"}],"value":"Fructose-1,6-bisphosphatase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00013093"}],"value":"3.1.3.11"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00032973"}],"value":"D-fructose-1,6-bisphosphate 1-phosphohydrolase"}}]},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77359.1"}],"value":"ACJ72_08345"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:11064"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:32966"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57634"}],"ecNumber":"3.1.3.11","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001273"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10043"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00024331"}],"value":"Carbohydrate biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011881"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010941"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000508"}],"value":"Belongs to the FBPase class 1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77359.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"Fructose-1-6-bisphosphatase class I N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00316"}]},{"type":"Domain","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":349,"modifier":"EXACT"}},"description":"Fructose-1-6-bisphosphatase class 1 C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF18913"}]},{"type":"Active site","location":{"start":{"value":291,"modifier":"EXACT"},"end":{"value":291,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10043"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023277"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000508"}],"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000508"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1CMFN1LMBD5LV","citationType":"submission","authors":["Cuomo C.A.","Schwartz I.S.","Kenyon C.","de Hoog G.S.","Govender N.P.","Botha A.","Moreno L.","de Vries M.","Munoz J.F.","Stielow J.B."],"title":"Emmonsia species relationships and genome sequence.","publicationDate":"JUL-2015","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77359.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}],"value":"CBS 136260","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"OAX77359.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000091918"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LGUA01002709","properties":[{"key":"ProteinId","value":"OAX77359.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A1B7NKJ6","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1658172.A0A1B7NKJ6","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"10256725at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000091918","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0042132","properties":[{"key":"GoTerm","value":"F:fructose 1,6-bisphosphate 1-phosphatase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0030388","properties":[{"key":"GoTerm","value":"P:fructose 1,6-bisphosphate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006002","properties":[{"key":"GoTerm","value":"P:fructose 6-phosphate metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006000","properties":[{"key":"GoTerm","value":"P:fructose metabolic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0006094","properties":[{"key":"GoTerm","value":"P:gluconeogenesis"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005986","properties":[{"key":"GoTerm","value":"P:sucrose biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd00354","properties":[{"key":"EntryName","value":"FBPase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.540.10:FF:000009","properties":[{"key":"EntryName","value":"Fructose-1,6-bisphosphatase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.190.80:FF:000001","properties":[{"key":"EntryName","value":"Fructose-1,6-bisphosphatase class 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.190.80","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.540.10","properties":[{"key":"EntryName","value":"Fructose-1,6-Bisphosphatase, subunit A, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01855","properties":[{"key":"EntryName","value":"FBPase_class1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR044015","properties":[{"key":"EntryName","value":"FBPase_C_dom"}]},{"database":"InterPro","id":"IPR000146","properties":[{"key":"EntryName","value":"FBPase_class-1"}]},{"database":"InterPro","id":"IPR033391","properties":[{"key":"EntryName","value":"FBPase_N"}]},{"database":"InterPro","id":"IPR028343","properties":[{"key":"EntryName","value":"FBPtase"}]},{"database":"InterPro","id":"IPR020548","properties":[{"key":"EntryName","value":"Fructose_bisphosphatase_AS"}]},{"database":"PANTHER","id":"PTHR11556","properties":[{"key":"EntryName","value":"FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11556:SF1","properties":[{"key":"EntryName","value":"FRUCTOSE-BISPHOSPHATASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00316","properties":[{"key":"EntryName","value":"FBPase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18913","properties":[{"key":"EntryName","value":"FBPase_C"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF500210","properties":[{"key":"EntryName","value":"FBPtase"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000904","properties":[{"key":"EntryName","value":"FBPtase_SBPase"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00115","properties":[{"key":"EntryName","value":"F16BPHPHTASE"}]},{"database":"SUPFAM","id":"SSF56655","properties":[{"key":"EntryName","value":"Carbohydrate phosphatase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00124","properties":[{"key":"EntryName","value":"FBPASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MADANGSSTPPVGQEKINTDIISLTRFLTEEQSKHPEATGDFTLLCHALQFSFKSISYYIRRATLINLTGLAGSSNTTGDDQKKLDVIGNDLFIAAMRGSGKCRILVSEEEEEAIIFDESPDARYAVVCDPIDGSSNLDAGVSVGTIFGIFKLPDEVLGPNSTVTAKDVLLPGTELVASGFTMYGASAQLVITMKGGTVNGFTMDTSLGEFILTHPNMRLPAKRAIYSVNEGNSMYWDNWVLEYFKDLKYPDNGKPYSSRYIGSMVADAYRTLLYGGVFAYPADKKSPKGKLRILYECAPMALVFENAGGQALNSHMERLLNITPEHIHDRSGVFLGSYEEVEKVKAFHQKYAK","length":354,"molWeight":38797,"crc64":"142F1C6AB7ABB563","md5":"91946A6DE80CAA0E21DAD32F513F6CCD"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"COFACTOR":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Domain":2,"Active site":1},"uniParcId":"UPI0007FF336E"}}]}