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site":16,"Modified residue":5,"Mutagenesis":15,"Beta strand":32,"Helix":47,"Turn":5},"uniParcId":"UPI00308E4BDF"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"E0D207","uniProtkbId":"GFSF_STRHA","entryAudit":{"firstPublicDate":"2025-02-05","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2010-11-02","entryVersion":51,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Streptomyces halstedii","taxonId":1944,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Kitasatosporales","Streptomycetaceae","Streptomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Cytochrome P450 GfsF"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20589823"}],"value":"1.14.-.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20589823"}],"value":"P450 monooxygenase 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Consecutively catalyzes epoxidation of C8-C9 and then hydroxylation at C10 to convert 25-O-methyl-FD-892 to FD-891 (PubMed:20589823). Consecutively catalyzes epoxidation of C8-C9 and then hydroxylation at C10 to convert 8,9-epoxy-FD-892 to 25-O-demethyl-FD-891 as well as converting 25-oxo-FD-892 to 8,9-epoxy-25-oxo-FD-892 and 8,9-epoxy-10-hydroxy-25-oxo-FD-892 (PubMed:26630077). In vitro is furnished with P.putida putidaredoxin and putidaredoxin reductase to provide the required two-electron reduction (PubMed:20589823, PubMed:26630077, PubMed:28869713)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"heme b","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28869713"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5Y1I"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:60344"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20589823"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26630077"}],"value":"Antibiotic biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28869713"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20589823"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26630077"}],"value":"No longer produces the 16-membered macrolide antibiotics FD-891 or FD-892 and instead makes 25-O-methyl-FD-892 (PubMed:20589823). Double gfsF-gfsG deletions make a diastereomeric mxiture of 25-oxo-FD-892 (PubMed:26630077)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8002384"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.4"}],"value":"The macrolide antibiotics FD-891 and FD-892 induce morphological changes of human promyelocytic leukemia (HL-60) cells and have cytocidal activity against tumor cell lines in vitro (PubMed:8002384). FD-891 produced by Streptomyces sp. 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