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There is one Pup binding site per ARC hexamer ring"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02106"}],"value":"The N-terminal unstructured half of Pup provides a signal required to initiate unfolding and degradation by the proteasome but is not needed for pupylation, while the C-terminal helical half of Pup interacts with ARC to target proteins to the proteasome"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02106"}],"value":"Is modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010616"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02106"}],"value":"Belongs to the prokaryotic ubiquitin-like protein family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02106"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"MPV35713.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Deamidated glutamine","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02106"}]},{"type":"Cross-link","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in acceptor proteins)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02106"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02106"}],"id":"KW-1017","category":"PTM","name":"Isopeptide bond"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000437709"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_02106"}],"id":"KW-0833","category":"Biological process","name":"Ubl conjugation pathway"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-4SD2BI5E3Q52D","citationType":"submission","authors":["Tian Z."],"title":"Georgenia wutianyii sp. nov. and Georgenia yuyongxinii sp. nov. isolated from plateau pika (Ochotona curzoniae) in the Qinghai-Tibet plateau of China.","publicationDate":"OCT-2019","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"MPV35713.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000437709"}],"value":"JCM 19765","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"MPV35713.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000437709"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"WHPC01000003","properties":[{"key":"ProteinId","value":"MPV35713.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_152195202.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_VUKD01000003.1"}]},{"database":"AlphaFoldDB","id":"A0A6N7EEW1","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"3254977at2","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00997","properties":[{"key":"RectionId","value":"-"}]},{"database":"Proteomes","id":"UP000437709","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0070628","properties":[{"key":"GoTerm","value":"F:proteasome binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0031386","properties":[{"key":"GoTerm","value":"F:protein tag activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0019941","properties":[{"key":"GoTerm","value":"P:modification-dependent protein catabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0010498","properties":[{"key":"GoTerm","value":"P:proteasomal protein catabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0070490","properties":[{"key":"GoTerm","value":"P:protein pupylation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_02106","properties":[{"key":"EntryName","value":"Pup"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008515","properties":[{"key":"EntryName","value":"Ubiquitin-like_Pup"}]},{"database":"NCBIfam","id":"TIGR03687","properties":[{"key":"EntryName","value":"pupylate_cterm"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05639","properties":[{"key":"EntryName","value":"Pup"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAEQEQRRTTRRDDPGPEPIDPPVAAAAQARTQEVDSLLDEIDSVLEANAESFVQGFVQKGGQ","length":63,"molWeight":6866,"crc64":"F3BB10BF9A488DCD","md5":"C94FFCF32B391351D03B78C6E9F733EF"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"PATHWAY":1,"SUBUNIT":1,"DOMAIN":1,"PTM":1,"SIMILARITY":1,"CAUTION":2},"countByFeatureType":{"Region":1,"Compositional bias":1,"Modified residue":1,"Cross-link":1},"uniParcId":"UPI0012648D69"}}]}