{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A0B4UK64","uniProtkbId":"A0A0B4UK64_9ASPA","entryAudit":{"firstPublicDate":"2015-04-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2015-04-01","entryVersion":26,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Xanthorrhoea nana","taxonId":1592189,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09001.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","Liliopsida","Asparagales","Asphodelaceae","Xanthorrhoeoideae","Xanthorrhoea"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00018648"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NADH-plastoquinone oxidoreductase subunit 5"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09001.1"}],"value":"ndhF"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004059"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42608"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048026"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42612"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047726"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011199"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004454"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Plastid, chloroplast thylakoid membrane","id":"SL-0058"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004454"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008200"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Belongs to the complex I subunit 5 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":128,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Domain","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"NADH:ubiquinone/plastoquinone oxidoreductase chloroplast chain 5 C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01010"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09001.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09001.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09001.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09001.1"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022957"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0618","category":"Ligand","name":"Plastoquinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022719"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0874","category":"PTM","name":"Quinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023078"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0793","category":"Cellular component","name":"Thylakoid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"25523814","citationType":"journal article","authors":["Crisp M.D.","Hardy N.B.","Cook L.G."],"citationCrossReferences":[{"database":"PubMed","id":"25523814"}],"title":"Clock model makes a large difference to age estimates of long-stemmed clades with no internal calibration: a test using Australian grasstrees.","publicationDate":"2014","journal":"BMC Evol. Biol.","firstPage":"263","lastPage":"263","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09001.1"}],"value":"Xan_nan9687","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09001.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KP227893","properties":[{"key":"ProteinId","value":"AJD09001.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A0B4UK64","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0009535","properties":[{"key":"GoTerm","value":"C:chloroplast thylakoid membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0042773","properties":[{"key":"GoTerm","value":"P:ATP synthesis coupled electron transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0015990","properties":[{"key":"GoTerm","value":"P:electron transport coupled proton transport"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"InterPro","id":"IPR002128","properties":[{"key":"EntryName","value":"NADH_UbQ_OxRdtase_chlpt_su5_C"}]},{"database":"InterPro","id":"IPR003945","properties":[{"key":"EntryName","value":"NU5C-like"}]},{"database":"PANTHER","id":"PTHR42829","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42829:SF2","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01010","properties":[{"key":"EntryName","value":"Proton_antipo_C"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"ASFTAGLTAFYMFRMYLLTFDGHLRIHFQNYSSTKNSSFYSISLWGKEAPKAINRNLLLSIMNNKVSFFSKDTYQIDDNVRNGIRYFSTYFRNKYTYTYPHESDNTMLFPLLVLVLFTLFVGSIGIHFDRGVIDFDILSKWLTPSTNFFHPNSNDSSDWYDFLKSAVSSVSIAILGLFIASILYGSVYSSLQNLDLTNSFVKRNPKRIYLDQIKNVIYNW","length":220,"molWeight":25561,"crc64":"9D65FD01DBD199C7","md5":"63B04A159D81C17A6FA0FB2F4155FFE0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Transmembrane":2,"Domain":1,"Non-terminal residue":2},"uniParcId":"UPI0005864114"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A0B4UKH5","uniProtkbId":"A0A0B4UKH5_9ASPA","entryAudit":{"firstPublicDate":"2015-04-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2015-04-01","entryVersion":31,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Xanthorrhoea nana","taxonId":1592189,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09096.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","Liliopsida","Asparagales","Asphodelaceae","Xanthorrhoeoideae","Xanthorrhoea"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012418"}],"value":"DNA-directed RNA polymerase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012418"}],"value":"2.7.7.6"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09096.1"}],"value":"rpb2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006835"}],"value":"Belongs to the RNA polymerase beta chain family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"DNA-directed RNA polymerase subunit 2 hybrid-binding","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00562"}]},{"type":"Region","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09096.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":144,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09096.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022478"}],"id":"KW-0240","category":"Cellular component","name":"DNA-directed RNA polymerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022695"}],"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023163"}],"id":"KW-0804","category":"Biological process","name":"Transcription"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"25523814","citationType":"journal article","authors":["Crisp M.D.","Hardy N.B.","Cook L.G."],"citationCrossReferences":[{"database":"PubMed","id":"25523814"}],"title":"Clock model makes a large difference to age estimates of long-stemmed clades with no internal calibration: a test using Australian grasstrees.","publicationDate":"2014","journal":"BMC Evol. Biol.","firstPage":"263","lastPage":"263","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09096.1"}],"value":"Xan_nan9687","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09096.1"}]},{"referenceNumber":2,"citation":{"id":"CI-BS66396L6L7SP","citationType":"submission","authors":["Toon A."],"publicationDate":"DEC-2014","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09096.1"}],"value":"Xan_nan9687","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AJD09096.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KP227987","properties":[{"key":"ProteinId","value":"AJD09095.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KP227988","properties":[{"key":"ProteinId","value":"AJD09096.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A0B4UKH5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003899","properties":[{"key":"GoTerm","value":"F:DNA-directed RNA polymerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0032549","properties":[{"key":"GoTerm","value":"F:ribonucleoside binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006351","properties":[{"key":"GoTerm","value":"P:DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"2.40.50.150","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR015712","properties":[{"key":"EntryName","value":"DNA-dir_RNA_pol_su2"}]},{"database":"InterPro","id":"IPR007120","properties":[{"key":"EntryName","value":"DNA-dir_RNAP_su2_dom"}]},{"database":"InterPro","id":"IPR014724","properties":[{"key":"EntryName","value":"RNA_pol_RPB2_OB-fold"}]},{"database":"PANTHER","id":"PTHR20856","properties":[{"key":"EntryName","value":"DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00562","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_6"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF64484","properties":[{"key":"EntryName","value":"beta and beta-prime subunits of DNA dependent RNA-polymerase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"DTLAYVLYYPQKPLVTTRAMEHLHFRQLPAGINAIVAIACYSGYNQEDSVIMNQSSIDRGFFRSLFFRSYRDEEKKMGTLVKEDFGRPNRENTMGMRHGSYDKLDDDGLAPPGTRVSGEDVIIGKTSPIAQDDSQGQASRYTRR","length":144,"molWeight":16317,"crc64":"184B12576AC81505","md5":"69DB10434C31A145FF923C17D256A6A2"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Region":1,"Compositional bias":1,"Non-terminal residue":2},"uniParcId":"UPI000585F63C"}}]}