{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"B1A612","uniProtkbId":"MENC_GEOKU","entryAudit":{"firstPublicDate":"2022-02-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-04-08","entryVersion":69,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Geobacillus kaustophilus","taxonId":1462,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Anoxybacillaceae","Geobacillus","Geobacillus thermoleovorans group"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01933"}],"value":"o-succinylbenzoate synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01933"}],"value":"OSB synthase"},{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01933"}],"value":"OSBS"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01933"}],"value":"4.2.1.113"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01933"}],"value":"4-(2'-carboxyphenyl)-4-oxybutyric acid synthase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.1"}],"value":"N-acylamino acid racemase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.1"}],"value":"NAAAR"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.1"}],"value":"N-succinylamino acid racemase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.1"}],"value":"NSAAR"},{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"NSAR"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25875730"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.1"}],"value":"5.1.1.-"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01933"}],"value":"o-succinylbenzoic acid synthase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01933"}],"value":"menC"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.1"}],"value":"nsaar"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q5L1G9"},{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01933"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25875730"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.1"}],"value":"Converts 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) to 2-succinylbenzoate (OSB) (By similarity). Also acts as a N-succinylamino acid racemase (NSAR) that catalyzes the racemization of various N-succinylamino acids, including N-succinyl-alanine and N-succinyl-phenylalanine (Ref.1). Can catalyze the racemization of a broad range of N-acylamino acids, including N-acetyl-methionine, N-acetyl-phenylalanine, N-carbamoyl-methionine, N-formyl-D-methionine, N-formyl-D-norleucine and N-carbamoyl-D-norleucine (PubMed:25875730, Ref.1). 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Sulfhydryl group-blocking reagents p-chloromercuribenzoic acid and iodoacetic acid inhibit 86.2 and 74% of the enzyme activity, respectively"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":6.62,"unit":"umol/min/mg","enzyme":"enzyme with ONPG as substrate (at 55 degrees Celsius and pH 7.0)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18420605"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3086288"}]}],"michaelisConstants":[{"constant":2.96,"unit":"mM","substrate":"ONPG (at 55 degrees Celsius and pH 7.0)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18420605"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3086288"}]}]},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18420605"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"3086288"}],"value":"Optimum pH is 7.0. 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