{"results":[{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"G1C9C2","uniProtkbId":"G1C9C2_9ASTR","entryAudit":{"firstPublicDate":"2011-10-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-10-19","entryVersion":40,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Volutaria crupinoides","taxonId":145832,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90456.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","asterids","campanulids","Asterales","Asteraceae","Carduoideae","Cardueae","Centaureinae","Volutaria"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00018648"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"7.1.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NADH-plastoquinone oxidoreductase subunit 5"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90456.1"}],"value":"ndhF"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004059"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42608"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048026"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42612"},{"database":"Rhea","id":"RHEA-COMP:9561"},{"database":"Rhea","id":"RHEA-COMP:9562"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17757"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:62192"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047726"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011199"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004454"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Plastid, chloroplast thylakoid membrane","id":"SL-0058"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004454"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008200"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Belongs to the complex I subunit 5 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":226,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":303,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Transmembrane","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":420,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}]},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"NADH:quinone oxidoreductase/Mrp antiporter transmembrane","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00361"}]},{"type":"Domain","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"NADH:ubiquinone/plastoquinone oxidoreductase chloroplast chain 5 C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF01010"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90456.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90456.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022528"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022857"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0521","category":"Ligand","name":"NADP"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022640"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022957"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0618","category":"Ligand","name":"Plastoquinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022719"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0874","category":"PTM","name":"Quinone"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023078"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0793","category":"Cellular component","name":"Thylakoid"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022967"}],"id":"KW-1278","category":"Molecular function","name":"Translocase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022448"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU364062"}],"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"21712295","citationType":"journal article","authors":["Susanna A.","Galbany-Casals M.","Romaschenko K.","Barres L.","Martin J.","Garcia-Jacas N."],"citationCrossReferences":[{"database":"PubMed","id":"21712295"},{"database":"DOI","id":"10.1093/aob/mcr138"}],"title":"Lessons from Plectocephalus (Compositae, Cardueae-Centaureinae): ITS disorientation in annuals and Beringian dispersal as revealed by molecular analyses.","publicationDate":"2011","journal":"Ann. Bot.","firstPage":"263","lastPage":"277","volume":"108"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90456.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"JF754867","properties":[{"key":"ProteinId","value":"AEJ90456.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"GO","id":"GO:0009535","properties":[{"key":"GoTerm","value":"C:chloroplast thylakoid membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008137","properties":[{"key":"GoTerm","value":"F:NADH dehydrogenase (ubiquinone) activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0042773","properties":[{"key":"GoTerm","value":"P:ATP synthesis coupled electron transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0015990","properties":[{"key":"GoTerm","value":"P:electron transport coupled proton transport"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"Gene3D","id":"1.20.5.2700","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002128","properties":[{"key":"EntryName","value":"NADH_UbQ_OxRdtase_chlpt_su5_C"}]},{"database":"InterPro","id":"IPR001750","properties":[{"key":"EntryName","value":"ND/Mrp_TM"}]},{"database":"InterPro","id":"IPR003945","properties":[{"key":"EntryName","value":"NU5C-like"}]},{"database":"PANTHER","id":"PTHR42829","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR42829:SF2","properties":[{"key":"EntryName","value":"NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01010","properties":[{"key":"EntryName","value":"Proton_antipo_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00361","properties":[{"key":"EntryName","value":"Proton_antipo_M"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01434","properties":[{"key":"EntryName","value":"NADHDHGNASE5"}]},{"database":"PRINTS","id":"PR01435","properties":[{"key":"EntryName","value":"NPOXDRDTASE5"}]}],"sequence":{"value":"TMSQLGYMMLALGMGSYRSALFHLITHAYSKALLFLGSGSVIHSMETLVGYCPKKSQNMVLMGGLTKHVPITKTSFLLGTLSLCGIPPLACFWSKDEILNDSWLYSPIFAIIAWSTAGLTAFYMCRIYLLTFEGHLNVHFQNYSGKKNTPFYSISLWGKEGSKISNKNFRLVTLXKMKKNGRVSFFSNKVYKIDENVRNMIQPFLSIPHFGNTKTYSYPYESDNTMLFPILILIIFTLFVGFLGIPFNQDGVNLDILSKWLTPSINLLHKNSNNSIDWYEFCKDAVFSVSIASFGIFIAFFLYKPVYSSFQNLDLINSFVKMGPKRIFSDKIKNGIYDWSYNRGYIDAFYGTFLTVGMRKLAKFAHFFDRRIIDGIPNGAGLMSFFVAEVIKSVGGGRISSYLFFYFSYVSICLLSYYFFNL","length":422,"molWeight":48079,"crc64":"C4C238C5284BF40B","md5":"8239CE037399DFA7582A9329C8AD586C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Transmembrane":5,"Domain":2,"Non-terminal residue":1},"uniParcId":"UPI000218E69C"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"G1C9G0","uniProtkbId":"G1C9G0_9ASTR","entryAudit":{"firstPublicDate":"2011-10-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-10-19","entryVersion":17,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Volutaria crupinoides","taxonId":145832,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","asterids","campanulids","Asterales","Asteraceae","Carduoideae","Cardueae","Centaureinae","Volutaria"]},"proteinExistence":"4: Predicted","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}],"value":"Ribosomal protein L32"}}],"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}],"value":"rpl32"}}],"features":[{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}]}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}],"geneEncodingType":"Chloroplast"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}],"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}],"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}],"id":"KW-0687","category":"Molecular function","name":"Ribonucleoprotein"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}],"id":"KW-0689","category":"Molecular function","name":"Ribosomal protein"}],"references":[{"referenceNumber":1,"citation":{"id":"21712295","citationType":"journal article","authors":["Susanna A.","Galbany-Casals M.","Romaschenko K.","Barres L.","Martin J.","Garcia-Jacas N."],"citationCrossReferences":[{"database":"PubMed","id":"21712295"},{"database":"DOI","id":"10.1093/aob/mcr138"}],"title":"Lessons from Plectocephalus (Compositae, Cardueae-Centaureinae): ITS disorientation in annuals and Beringian dispersal as revealed by molecular analyses.","publicationDate":"2011","journal":"Ann. Bot.","firstPage":"263","lastPage":"277","volume":"108"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AEJ90494.1"}]},{"referenceNumber":2,"citation":{"id":"CI-FEKHS1JOM0OBQ","citationType":"journal article","authors":["Calleja J.A.","Garcia-Jacas N.","Roquet C.","Susanna A."],"title":"Beyond the Rand Flora pattern: Phylogeny and biogeographical history of Volutaria (Compositae).","publicationDate":"2016","journal":"Taxon","firstPage":"315","lastPage":"332","volume":"65"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ANS53223.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"JF754905","properties":[{"key":"ProteinId","value":"AEJ90494.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KU309439","properties":[{"key":"ProteinId","value":"ANS53223.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KU309441","properties":[{"key":"ProteinId","value":"ANS53225.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KU309442","properties":[{"key":"ProteinId","value":"ANS53226.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KU309443","properties":[{"key":"ProteinId","value":"ANS53227.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"KU309444","properties":[{"key":"ProteinId","value":"ANS53228.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"G1C9G0","properties":[{"key":"Description","value":"-"}]},{"database":"InterPro","id":"IPR044958","properties":[{"key":"EntryName","value":"Ribosomal_bL32_plant_cyanobact"}]},{"database":"PANTHER","id":"PTHR36083","properties":[{"key":"EntryName","value":"50S RIBOSOMAL PROTEIN L32, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR36083:SF1","properties":[{"key":"EntryName","value":"LARGE RIBOSOMAL SUBUNIT PROTEIN BL32C"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"GYWAALKALSLGKSLSTGNSKSFFVRQTNKS","length":31,"molWeight":3348,"crc64":"1A05F5E4914E01BB","md5":"C0B7EC162AB9E99D4D7DA2D094D58981"},"extraAttributes":{"countByFeatureType":{"Non-terminal residue":1},"uniParcId":"UPI0001B197EF"}}]}