{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P43269","uniProtkbId":"NUC_STAIN","entryAudit":{"firstPublicDate":"1995-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1995-11-01","entryVersion":93,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Thermonuclease"},"shortNames":[{"value":"TNase"}],"ecNumbers":[{"value":"3.1.31.1"}]},"alternativeNames":[{"fullName":{"value":"Micrococcal nuclease"}},{"fullName":{"value":"Staphylococcal nuclease"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"nucI"},"synonyms":[{"value":"nuc"}]}],"comments":[{"texts":[{"value":"Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5'-position of the phosphodiester bond"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.","ecNumber":"3.1.31.1","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10048"},{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10049"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Ca(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29108"}}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00272"}],"value":"Belongs to the thermonuclease family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"Thermonuclease","featureId":"PRO_0000034393"},{"type":"Active site","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":114,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]}],"keywords":[{"id":"KW-0106","category":"Ligand","name":"Calcium"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"1408843","citationType":"journal article","authors":["Chesneau O.","el Solh N."],"citationCrossReferences":[{"database":"PubMed","id":"1408843"},{"database":"DOI","id":"10.1093/nar/20.19.5232"}],"title":"Nucleotide sequence of a nuc gene encoding the thermonuclease of Staphylococcus intermedius.","publicationDate":"1992","journal":"Nucleic Acids Res.","firstPage":"5232","lastPage":"5232","volume":"20"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"LRA076","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X67678","properties":[{"key":"ProteinId","value":"CAA47910.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S26079","properties":[{"key":"EntryName","value":"S26079"}]},{"database":"AlphaFoldDB","id":"P43269","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P43269","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1525","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:1990599","properties":[{"key":"GoTerm","value":"F:3' overhang single-stranded DNA endonuclease activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0003676","properties":[{"key":"GoTerm","value":"F:nucleic acid binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00175","properties":[{"key":"EntryName","value":"SNc"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.90","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR035437","properties":[{"key":"EntryName","value":"SNase_OB-fold_sf"}]},{"database":"InterPro","id":"IPR016071","properties":[{"key":"EntryName","value":"Staphylococal_nuclease_OB-fold"}]},{"database":"InterPro","id":"IPR002071","properties":[{"key":"EntryName","value":"Thermonucl_AS"}]},{"database":"NCBIfam","id":"NF047694","properties":[{"key":"EntryName","value":"TnucaseNucIStaph"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12302","properties":[{"key":"EntryName","value":"EBNA2 BINDING PROTEIN P100"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12302:SF3","properties":[{"key":"EntryName","value":"SERINE_THREONINE-PROTEIN KINASE 31"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00565","properties":[{"key":"EntryName","value":"SNase"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00318","properties":[{"key":"EntryName","value":"SNc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50199","properties":[{"key":"EntryName","value":"Staphylococcal nuclease"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01123","properties":[{"key":"EntryName","value":"TNASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01284","properties":[{"key":"EntryName","value":"TNASE_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50830","properties":[{"key":"EntryName","value":"TNASE_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKKITTGVLILAIAIVVLIFQYINGDGPFKKSSTDVRGESYLVKRVIDGDTIIIDKDGQDERVRLIGVDTPETVKPNTPVQPYGKAASNFTKKHLTNQRVRLEYDREPKDKYGRTLAYVWLGDEMFNVKLAKEGLARAKFYPPNDKYRILIEQAQKEAQKKQLNIWER","length":168,"molWeight":19299,"crc64":"872063F4B32DD911","md5":"12245D825B0E6AB60E6BE9D2D926FFE8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Active site":3},"uniParcId":"UPI00001308DE"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P80924","uniProtkbId":"PHLC_STAIN","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":60,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Sphingomyelinase C"},"ecNumbers":[{"value":"3.1.4.12"}]},"alternativeNames":[{"fullName":{"value":"Beta-hemolysin"}},{"fullName":{"value":"Beta-toxin"}},{"fullName":{"value":"Neutral sphingomyelinase"},"shortNames":[{"value":"nSMase"}]}],"flag":"Fragment"},"comments":[{"texts":[{"value":"Required, with sphingomyelinase, to effect target cell lysis (hemolysis)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a sphingomyelin + H2O = phosphocholine + an N-acylsphing-4-enine + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19253"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17636"},{"database":"ChEBI","id":"CHEBI:52639"},{"database":"ChEBI","id":"CHEBI:295975"}],"ecNumber":"3.1.4.12"}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","phDependence":{"texts":[{"value":"Optimum pH is 6.0-7.5."}]}},{"texts":[{"value":"Monomer"}],"commentType":"SUBUNIT"},{"texts":[{"value":"It has a high specificity for sphingomyelin, hydrolyzes lysophosphatidylcholine at a much lower rate, but has no activity towards phosphatidylcholine, phosphatidylethanolamine, or phosphatidylserine"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the neutral sphingomyelinase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":19,"modifier":"OUTSIDE"}},"description":"Sphingomyelinase C","featureId":"PRO_0000075696"},{"type":"Non-terminal residue","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":""}],"keywords":[{"id":"KW-0204","category":"Biological process","name":"Cytolysis"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0354","category":"Biological process","name":"Hemolysis"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"8914839","citationType":"journal article","authors":["Dziewanowska K.","Edwards V.M.","Deringer J.R.","Bohach G.A.","Guerra D.J."],"citationCrossReferences":[{"database":"PubMed","id":"8914839"},{"database":"DOI","id":"10.1006/abbi.1996.0486"}],"title":"Comparison of the beta-toxins from Staphylococcus aureus and Staphylococcus intermedius.","publicationDate":"1996","journal":"Arch. Biochem. Biophys.","firstPage":"102","lastPage":"108","volume":"335"},"referencePositions":["PROTEIN SEQUENCE"],"referenceComments":[{"value":"94-072594","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"AlphaFoldDB","id":"P80924","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0004767","properties":[{"key":"GoTerm","value":"F:sphingomyelin phosphodiesterase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]}],"sequence":{"value":"ASGENQAELKLATHNVYML","length":19,"molWeight":2089,"crc64":"A50753FCF500F80E","md5":"C9AF5957ABA7A0A320860B93B2DD3AB7"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Non-terminal residue":1},"uniParcId":"UPI0000131981"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P25309","uniProtkbId":"CAT_STAIN","entryAudit":{"firstPublicDate":"1992-05-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1992-05-01","entryVersion":82,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Chloramphenicol acetyltransferase"},"shortNames":[{"value":"CAT"}],"ecNumbers":[{"value":"2.3.1.28"}]}},"genes":[{"geneName":{"value":"cat"}}],"comments":[{"texts":[{"value":"This enzyme is an effector of chloramphenicol resistance in bacteria"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"chloramphenicol + acetyl-CoA = chloramphenicol 3-acetate + CoA","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:18421"},{"database":"ChEBI","id":"CHEBI:16730"},{"database":"ChEBI","id":"CHEBI:17698"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:57288"}],"ecNumber":"2.3.1.28","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10021"}]}},{"texts":[{"value":"Homotrimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the chloramphenicol acetyltransferase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"Chloramphenicol acetyltransferase","featureId":"PRO_0000165869"},{"type":"Active site","location":{"start":{"value":189,"modifier":"EXACT"},"end":{"value":189,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10021"}]}],"geneLocations":[{"value":"pSCS1","geneEncodingType":"Plasmid"}],"keywords":[{"id":"KW-0012","category":"Molecular function","name":"Acyltransferase"},{"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"id":"KW-0614","category":"Technical term","name":"Plasmid"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"1713259","citationType":"journal article","authors":["Schwarz S.","Spies U.","Cardoso M."],"citationCrossReferences":[{"database":"PubMed","id":"1713259"},{"database":"DOI","id":"10.1099/00221287-137-4-977"}],"title":"Cloning and sequence analysis of a plasmid-encoded chloramphenicol acetyltransferase gene from Staphylococcus intermedius.","publicationDate":"1991","journal":"J. Gen. Microbiol.","firstPage":"977","lastPage":"981","volume":"137"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M64281","properties":[{"key":"ProteinId","value":"AAA26615.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A49758","properties":[{"key":"EntryName","value":"A49758"}]},{"database":"RefSeq","id":"WP_063843211.1","properties":[{"key":"NucleotideSequenceId","value":"NG_047572.1"}]},{"database":"AlphaFoldDB","id":"P25309","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P25309","properties":[{"key":"Description","value":"-"}]},{"database":"CARD","id":"ARO:3004457","properties":[{"key":"CARD short name","value":"Sint_ACT_CHL"},{"key":"Resistance mechanism identifier","value":"ARO:0001004"},{"key":"Resistance mechanism name","value":"antibiotic inactivation"}]},{"database":"GO","id":"GO:0008811","properties":[{"key":"GoTerm","value":"F:chloramphenicol O-acetyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"Gene3D","id":"3.30.559.10","properties":[{"key":"EntryName","value":"Chloramphenicol acetyltransferase-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR023213","properties":[{"key":"EntryName","value":"CAT-like_dom_sf"}]},{"database":"InterPro","id":"IPR018372","properties":[{"key":"EntryName","value":"Chloramphenicol_AcTrfase_AS"}]},{"database":"InterPro","id":"IPR001707","properties":[{"key":"EntryName","value":"Cmp_AcTrfase"}]},{"database":"NCBIfam","id":"NF000491","properties":[{"key":"EntryName","value":"chloram_CatA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR38474:SF2","properties":[{"key":"EntryName","value":"CHLORAMPHENICOL ACETYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR38474","properties":[{"key":"EntryName","value":"SLR0299 PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00302","properties":[{"key":"EntryName","value":"CAT"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000440","properties":[{"key":"EntryName","value":"CAT"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01059","properties":[{"key":"EntryName","value":"CAT"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52777","properties":[{"key":"EntryName","value":"CoA-dependent acyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00100","properties":[{"key":"EntryName","value":"CAT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTFNIIKLENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKKKGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDNNFTSFYNNYKNDLFEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHAVCDGYHASLFINEFQDIIKKVDDWI","length":215,"molWeight":25725,"crc64":"44D598FDEA06163A","md5":"677EF9284AB31A4C9F38143D9AFEB135"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1},"uniParcId":"UPI00001270E5"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P61649","uniProtkbId":"AGRB_STAIN","entryAudit":{"firstPublicDate":"2004-06-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-06-07","entryVersion":61,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Accessory gene regulator protein B"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}],"value":"3.4.-.-"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}],"value":"agrB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Essential for the production of a quorum sensing system signal molecule, the autoinducing peptide (AIP). This quorum sensing system is responsible for the regulation of the expression of virulence factor genes. Involved in the proteolytic processing of AgrD, the precursor of AIP"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Belongs to the AgrB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"Accessory gene regulator protein B","featureId":"PRO_0000168131"},{"type":"Transmembrane","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}]},{"type":"Transmembrane","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}]},{"type":"Transmembrane","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}]},{"type":"Transmembrane","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":186,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00784"}]}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-0673","category":"Biological process","name":"Quorum sensing"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-291763K7JF370","citationType":"submission","authors":["Pei W.","Ji G."],"title":"Staphylococcus intermedius agr gene locus.","publicationDate":"FEB-2004","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY557375","properties":[{"key":"ProteinId","value":"AAS66745.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_019167559.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_JBBLTU010000007.1"}]},{"database":"AlphaFoldDB","id":"P61649","properties":[{"key":"Description","value":"-"}]},{"database":"MEROPS","id":"C75.001","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG4512","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008233","properties":[{"key":"GoTerm","value":"F:peptidase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009372","properties":[{"key":"GoTerm","value":"P:quorum sensing"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_00784","properties":[{"key":"EntryName","value":"AgrB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006741","properties":[{"key":"EntryName","value":"AgrB"}]},{"database":"Pfam","id":"PF04647","properties":[{"key":"EntryName","value":"AgrB"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00793","properties":[{"key":"EntryName","value":"AgrB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLLIDNGIEKMALKLQQRQNLSHIEFLKVRLGMQVVVINTFKAIVTYGLALLLNIFLYTLIVHLTFLTLRTYSHGAHAKTSMLCHVQNIVAFVMLPWLIVQYDISFQFLLILSLLSALIVIKYAPAATKKRPIAPKKVKGLKIKSIIVFVLLMTIACIVPPPYNRFVVYGVLLQSFTLLPIFSIKEEV","length":188,"molWeight":21324,"crc64":"63A94383ADEFD1F9","md5":"A731CA34D9FDD10B0C321BC32A31A4EA"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":4},"uniParcId":"UPI000034EBD3"}},{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q5U8S9","uniProtkbId":"EFG_STAIN","entryAudit":{"firstPublicDate":"2005-07-19","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2007-01-23","entryVersion":99,"sequenceVersion":3},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"Elongation factor G"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"EF-G"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"fusA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":693,"modifier":"EXACT"}},"description":"Elongation factor G","featureId":"PRO_0000091223"},{"type":"Domain","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":282,"modifier":"EXACT"}},"description":"tr-type G"},{"type":"Binding site","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":135,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00054"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0251","category":"Molecular function","name":"Elongation factor"},{"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"17325218","citationType":"journal article","authors":["O'Neill A.J.","McLaws F.","Kahlmeter G.","Henriksen A.S.","Chopra I."],"citationCrossReferences":[{"database":"PubMed","id":"17325218"},{"database":"DOI","id":"10.1128/aac.01542-06"}],"title":"Genetic basis of resistance to fusidic acid in staphylococci.","publicationDate":"2007","journal":"Antimicrob. Agents Chemother.","firstPage":"1737","lastPage":"1740","volume":"51"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 29663 / DSM 20373 / NCTC 11048 / H11","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY776250","properties":[{"key":"ProteinId","value":"AAV39278.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_019169061.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_JBBLTU010000008.1"}]},{"database":"AlphaFoldDB","id":"Q5U8S9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q5U8S9","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0480","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003746","properties":[{"key":"GoTerm","value":"F:translation elongation factor activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd01886","properties":[{"key":"EntryName","value":"EF-G"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16262","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01434","properties":[{"key":"EntryName","value":"EFG_mtEFG1_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03713","properties":[{"key":"EntryName","value":"EFG_mtEFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04088","properties":[{"key":"EntryName","value":"EFG_mtEFG_II"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.30.10:FF:000006","properties":[{"key":"EntryName","value":"Elongation factor G"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.230.10:FF:000003","properties":[{"key":"EntryName","value":"Elongation factor G"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.870:FF:000001","properties":[{"key":"EntryName","value":"Elongation factor G"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000029","properties":[{"key":"EntryName","value":"Elongation factor G"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.870","properties":[{"key":"EntryName","value":"Elongation Factor G (Translational Gtpase), domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00054_B","properties":[{"key":"EntryName","value":"EF_G_EF_2_B"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR041095","properties":[{"key":"EntryName","value":"EFG_II"}]},{"database":"InterPro","id":"IPR009022","properties":[{"key":"EntryName","value":"EFG_III"}]},{"database":"InterPro","id":"IPR035647","properties":[{"key":"EntryName","value":"EFG_III/V"}]},{"database":"InterPro","id":"IPR047872","properties":[{"key":"EntryName","value":"EFG_IV"}]},{"database":"InterPro","id":"IPR035649","properties":[{"key":"EntryName","value":"EFG_V"}]},{"database":"InterPro","id":"IPR000640","properties":[{"key":"EntryName","value":"EFG_V-like"}]},{"database":"InterPro","id":"IPR004161","properties":[{"key":"EntryName","value":"EFTu-like_2"}]},{"database":"InterPro","id":"IPR031157","properties":[{"key":"EntryName","value":"G_TR_CS"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR005225","properties":[{"key":"EntryName","value":"Small_GTP-bd"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR004540","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2"}]},{"database":"InterPro","id":"IPR005517","properties":[{"key":"EntryName","value":"Transl_elong_EFG/EF2_IV"}]},{"database":"NCBIfam","id":"TIGR00484","properties":[{"key":"EntryName","value":"EF-G"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009381","properties":[{"key":"EntryName","value":"fusA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009379","properties":[{"key":"EntryName","value":"PRK12740.1-3"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00231","properties":[{"key":"EntryName","value":"small_GTP"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261:SF1","properties":[{"key":"EntryName","value":"RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43261","properties":[{"key":"EntryName","value":"TRANSLATION ELONGATION FACTOR G-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00679","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14492","properties":[{"key":"EntryName","value":"EFG_III"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03764","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03144","properties":[{"key":"EntryName","value":"GTP_EFTU_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"SMART","id":"SM00838","properties":[{"key":"EntryName","value":"EFG_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00889","properties":[{"key":"EntryName","value":"EFG_IV"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54980","properties":[{"key":"EntryName","value":"EF-G C-terminal domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00301","properties":[{"key":"EntryName","value":"G_TR_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGRDFSLKNTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRGITITSAATTAEWKNHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKIGANFDYAVSTLHDRLQANAAPIQLPIGAEDEFSAIIDLVTMKCFKYNNDLGTEIEEVEIPEDYRERAEEAREALIEAVAETNESLMEKIFDEQEITVDELKDAIRQATTDVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIVGHRADNPDEEVIAKADDDAEFAALAFKVMTDPYVGKLTFFRVYSGTLTSGSYVKNSTKGKRERVGRILQMHANSREEISSVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFKAHTDEETGQVIIGGMGELHLDIIVDRMKKEFNVEANVGAPMVSYRETFKTSAAVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEQGLKDAMENGVLAGYPLIDVKAKLFDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVTARRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSISEEIIKKNKGE","length":693,"molWeight":76731,"crc64":"EAEAA7A4149B3CD3","md5":"EF05611F6272C43BE2F4FA6AB6656182"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Domain":1,"Binding site":3},"uniParcId":"UPI0000455F2D"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B0B0K3","uniProtkbId":"B0B0K3_STAIN","entryAudit":{"firstPublicDate":"2008-02-26","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-02-26","entryVersion":102,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18881.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"value":"DNA gyrase subunit B"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"value":"5.6.2.2"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18881.1"}],"value":"gyrB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"value":"A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP-dependent breakage, passage and rejoining of double-stranded DNA.","ecNumber":"5.6.2.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000185"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}},{"name":"Ca(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29108"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"value":"Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00063644"}],"value":"Heterotetramer composed of ParC and ParE"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"value":"Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"value":"Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010708"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"value":"Belongs to the type II topoisomerase GyrB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":425,"modifier":"EXACT"},"end":{"value":539,"modifier":"EXACT"}},"description":"Toprim","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50880"}]},{"type":"Binding site","location":{"start":{"value":431,"modifier":"EXACT"},"end":{"value":431,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":504,"modifier":"EXACT"},"end":{"value":504,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":504,"modifier":"EXACT"},"end":{"value":504,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":506,"modifier":"EXACT"},"end":{"value":506,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Site","location":{"start":{"value":456,"modifier":"EXACT"},"end":{"value":456,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00995"}]},{"type":"Site","location":{"start":{"value":459,"modifier":"EXACT"},"end":{"value":459,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00995"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023125"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00995"}],"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023029"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01898"}],"id":"KW-0799","category":"Molecular function","name":"Topoisomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"18305127","citationType":"journal article","authors":["Descloux S.","Rossano A.","Perreten V."],"citationCrossReferences":[{"database":"PubMed","id":"18305127"},{"database":"DOI","id":"10.1128/JCM.02255-07"}],"title":"Characterization of new staphylococcal cassette chromosome mec (SCCmec) and topoisomerase genes in fluoroquinolone- and methicillin-resistant Staphylococcus pseudintermedius.","publicationDate":"2008","journal":"J. Clin. Microbiol.","firstPage":"1818","lastPage":"1823","volume":"46"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18881.1"}],"value":"DSM20373","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18881.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AM262967","properties":[{"key":"ProteinId","value":"CAK18881.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B0B0K3","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0187","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"GO","id":"GO:0005694","properties":[{"key":"GoTerm","value":"C:chromosome"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0034335","properties":[{"key":"GoTerm","value":"F:DNA negative supercoiling activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0006265","properties":[{"key":"GoTerm","value":"P:DNA topological change"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006261","properties":[{"key":"GoTerm","value":"P:DNA-templated DNA replication"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd16928","properties":[{"key":"EntryName","value":"HATPase_GyrB-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00822","properties":[{"key":"EntryName","value":"TopoII_Trans_DNA_gyrase"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd03366","properties":[{"key":"EntryName","value":"TOPRIM_TopoIIA_GyrB"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.230.10:FF:000005","properties":[{"key":"EntryName","value":"DNA gyrase subunit B"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.565.10:FF:000002","properties":[{"key":"EntryName","value":"DNA gyrase subunit B"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.670:FF:000002","properties":[{"key":"EntryName","value":"DNA gyrase subunit B"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.670","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.565.10","properties":[{"key":"EntryName","value":"Histidine kinase-like ATPase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01898","properties":[{"key":"EntryName","value":"GyrB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002288","properties":[{"key":"EntryName","value":"DNA_gyrase_B_C"}]},{"database":"InterPro","id":"IPR011557","properties":[{"key":"EntryName","value":"GyrB"}]},{"database":"InterPro","id":"IPR036890","properties":[{"key":"EntryName","value":"HATPase_C_sf"}]},{"database":"InterPro","id":"IPR003594","properties":[{"key":"EntryName","value":"HATPase_dom"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR001241","properties":[{"key":"EntryName","value":"Topo_IIA"}]},{"database":"InterPro","id":"IPR013760","properties":[{"key":"EntryName","value":"Topo_IIA-like_dom_sf"}]},{"database":"InterPro","id":"IPR000565","properties":[{"key":"EntryName","value":"Topo_IIA_B"}]},{"database":"InterPro","id":"IPR013759","properties":[{"key":"EntryName","value":"Topo_IIA_B_C"}]},{"database":"InterPro","id":"IPR013506","properties":[{"key":"EntryName","value":"Topo_IIA_bsu_dom2"}]},{"database":"InterPro","id":"IPR018522","properties":[{"key":"EntryName","value":"TopoIIA_CS"}]},{"database":"InterPro","id":"IPR006171","properties":[{"key":"EntryName","value":"TOPRIM_dom"}]},{"database":"InterPro","id":"IPR034160","properties":[{"key":"EntryName","value":"TOPRIM_GyrB"}]},{"database":"NCBIfam","id":"TIGR01059","properties":[{"key":"EntryName","value":"gyrB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF004189","properties":[{"key":"EntryName","value":"PRK05644.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF011501","properties":[{"key":"EntryName","value":"PRK14939.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45866:SF1","properties":[{"key":"EntryName","value":"DNA GYRASE SUBUNIT B, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45866","properties":[{"key":"EntryName","value":"DNA GYRASE/TOPOISOMERASE SUBUNIT B"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00204","properties":[{"key":"EntryName","value":"DNA_gyraseB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00986","properties":[{"key":"EntryName","value":"DNA_gyraseB_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02518","properties":[{"key":"EntryName","value":"HATPase_c"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01751","properties":[{"key":"EntryName","value":"Toprim"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01159","properties":[{"key":"EntryName","value":"DNAGYRASEB"}]},{"database":"PRINTS","id":"PR00418","properties":[{"key":"EntryName","value":"TPI2FAMILY"}]},{"database":"SMART","id":"SM00387","properties":[{"key":"EntryName","value":"HATPase_c"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00433","properties":[{"key":"EntryName","value":"TOP2c"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55874","properties":[{"key":"EntryName","value":"ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56719","properties":[{"key":"EntryName","value":"Type II DNA topoisomerase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00177","properties":[{"key":"EntryName","value":"TOPOISOMERASE_II"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50880","properties":[{"key":"EntryName","value":"TOPRIM"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MADVNNSENYGASQIQVLEGLEAVRKRPGMYIGSTSERGLHHLVWEIVDNSIDEALAGYADNIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVILTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSDTLEVYVHRDGRIHHQAYHKGVPAFDLKQVGDTDQTGTVIRFKADGTIFQETTVYNYETLQKRIRELAFLNKGIRITLTDERDEENVREDSYHYEGGIKSYVELINENKEPLHEEPIYVHQTRDDIEVEIALQYNSGFATNLLTYANNIHTYEGGTHEDGFKRALTRVLNNYGAQSKLIKEDKDRLSGEDTREGLTAVVSIKHGDPQFEGQTKTKLGNSEVRQIVDRVFSELFERFLFEHPQVARIIVEKGIMASRARIAAKKAREVTRRKSALDISSLPGKLADCSSKDPSESEIFLVEGDSAGGSTKSGRDSRTQAILPLRGKILNVEKARLDKILSNNEIRQMVTAFGTGIGGEFDISKARYHKIVIMTDADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVFNDRELDKLKEKLNPTPKWSIARYKGLGEMNADQLWETTMNPENRAMLQVTLDDAIEADQTFEMLMGDVVENRRQFIEDNAVYANLDF","length":640,"molWeight":72005,"crc64":"2F956312E660578F","md5":"16FF2387B728D3D67DABA35B49D1EECA"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBUNIT":2,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Binding site":4,"Site":2},"uniParcId":"UPI000162F4C1"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B0B0K4","uniProtkbId":"B0B0K4_STAIN","entryAudit":{"firstPublicDate":"2008-02-26","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-02-26","entryVersion":97,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18882.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"value":"DNA gyrase subunit A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"value":"5.6.2.2"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18882.1"}],"value":"gyrA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"value":"A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP-dependent breakage, passage and rejoining of double-stranded DNA.","ecNumber":"5.6.2.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000185"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01384"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"value":"Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"value":"Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008263"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"value":"Belongs to the type II topoisomerase GyrA/ParC subunit family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":501,"modifier":"EXACT"}},"description":"Topo IIA-type catalytic","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52040"}]},{"type":"Region","location":{"start":{"value":811,"modifier":"EXACT"},"end":{"value":882,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled coil","location":{"start":{"value":438,"modifier":"EXACT"},"end":{"value":479,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Coils"}]},{"type":"Motif","location":{"start":{"value":528,"modifier":"EXACT"},"end":{"value":534,"modifier":"EXACT"}},"description":"GyrA-box","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}]},{"type":"Compositional bias","location":{"start":{"value":815,"modifier":"EXACT"},"end":{"value":840,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":849,"modifier":"EXACT"},"end":{"value":875,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01384"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022840"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Coils"}],"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023125"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022741"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023029"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01897"}],"id":"KW-0799","category":"Molecular function","name":"Topoisomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"18305127","citationType":"journal article","authors":["Descloux S.","Rossano A.","Perreten V."],"citationCrossReferences":[{"database":"PubMed","id":"18305127"},{"database":"DOI","id":"10.1128/JCM.02255-07"}],"title":"Characterization of new staphylococcal cassette chromosome mec (SCCmec) and topoisomerase genes in fluoroquinolone- and methicillin-resistant Staphylococcus pseudintermedius.","publicationDate":"2008","journal":"J. Clin. Microbiol.","firstPage":"1818","lastPage":"1823","volume":"46"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18882.1"}],"value":"DSM20373","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18882.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AM262967","properties":[{"key":"ProteinId","value":"CAK18882.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_019169019.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_JBBLTU010000004.1"}]},{"database":"AlphaFoldDB","id":"B0B0K4","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0188","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"GO","id":"GO:0005694","properties":[{"key":"GoTerm","value":"C:chromosome"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0009330","properties":[{"key":"GoTerm","value":"C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0034335","properties":[{"key":"GoTerm","value":"F:DNA negative supercoiling activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0006265","properties":[{"key":"GoTerm","value":"P:DNA topological change"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006261","properties":[{"key":"GoTerm","value":"P:DNA-templated DNA replication"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00187","properties":[{"key":"EntryName","value":"TOP4c"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.268.10:FF:000001","properties":[{"key":"EntryName","value":"DNA gyrase subunit A"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.120.10.90:FF:000004","properties":[{"key":"EntryName","value":"DNA gyrase subunit A"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.199.10:FF:000001","properties":[{"key":"EntryName","value":"DNA gyrase subunit A"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1360.40","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.120.10.90","properties":[{"key":"EntryName","value":"DNA gyrase/topoisomerase IV, subunit A, C-terminal"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.199.10","properties":[{"key":"EntryName","value":"Topoisomerase II, domain 5"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.268.10","properties":[{"key":"EntryName","value":"Topoisomerase, domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01897","properties":[{"key":"EntryName","value":"GyrA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005743","properties":[{"key":"EntryName","value":"GyrA"}]},{"database":"InterPro","id":"IPR006691","properties":[{"key":"EntryName","value":"GyrA/parC_rep"}]},{"database":"InterPro","id":"IPR035516","properties":[{"key":"EntryName","value":"Gyrase/topoIV_suA_C"}]},{"database":"InterPro","id":"IPR013760","properties":[{"key":"EntryName","value":"Topo_IIA-like_dom_sf"}]},{"database":"InterPro","id":"IPR013758","properties":[{"key":"EntryName","value":"Topo_IIA_A/C_ab"}]},{"database":"InterPro","id":"IPR013757","properties":[{"key":"EntryName","value":"Topo_IIA_A_a_sf"}]},{"database":"InterPro","id":"IPR002205","properties":[{"key":"EntryName","value":"Topo_IIA_dom_A"}]},{"database":"InterPro","id":"IPR050220","properties":[{"key":"EntryName","value":"Type_II_DNA_Topoisomerases"}]},{"database":"NCBIfam","id":"NF004043","properties":[{"key":"EntryName","value":"gyrA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01063","properties":[{"key":"EntryName","value":"gyrA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF004044","properties":[{"key":"EntryName","value":"PRK05561.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43493:SF5","properties":[{"key":"EntryName","value":"DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43493","properties":[{"key":"EntryName","value":"DNA GYRASE/TOPOISOMERASE SUBUNIT A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03989","properties":[{"key":"EntryName","value":"DNA_gyraseA_C"},{"key":"MatchStatus","value":"6"}]},{"database":"Pfam","id":"PF00521","properties":[{"key":"EntryName","value":"DNA_topoisoIV"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00434","properties":[{"key":"EntryName","value":"TOP4c"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF101904","properties":[{"key":"EntryName","value":"GyrA/ParC C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56719","properties":[{"key":"EntryName","value":"Type II DNA topoisomerase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52040","properties":[{"key":"EntryName","value":"TOPO_IIA"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAETPESRINERNISKEMRESFLDYAMSVIVSRALPDVRDGLKPVHRRILYGLNEQGMTPDKPYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYPLVDGQGNFGSMDGDGAAAMRYTEARMTKLALELLRDINKDTIDFIDNYDGNEREPSVLPSRFPNLLVNGASGIAVGMATNIPPHNMREVIDGVLSLSHNPDITISELMEDIQGPDFPTAGLILGKSGIRRAYETGRGSVIMRAKAEIESRGGGRERIVVTEIPFQVNKARMIEKIADLVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVNMIALVNGRPQLINLKQALYHYLEHQKEVVRRRTEYNLRKAKDRAHILEGLRIALDHIDEIITIIRESDTDKVAMESLQSRFALSERQAQAILDMRLRRLTGLERDKIEQEYNDLIAYIAELEAILADEEKLLELVREELTEIKEKFGDERRTEIQLGGIDQLEDEDLIPEEQIVITLSHNNYIKRLPASTYRAQNRGGRGVQGMNTLEDDFVSQLVTTSTHDHVLFFTNKGRVYKLKGYEVPELSRQSKGIPIVNAIELDQDEVISTMIAVKDLDSEEDFLVFVTKKGLIKRSALSNFNRINRNGKIAIKFRDDDELIAVRLTDGDKHILIGTAQASLIRFKETDVRAMSRIAAGVKGIRLRDGDEVIGLDVADDDNKDEILVVTEKGYGKRTSIEDYRLSNRGGMGVKTAKLTERNGRLVCITTVEGDEDLMVVTNQGVIIRMEVSNISVNGRMAQGVRLIRLDDEQYVSTVAKVKKEPEDLESDEDTGVSEVSDVADDATEVVVDDATPGDTIHTEAPEPEASPERETLREDFMDRVNEDIENEDE","length":882,"molWeight":99230,"crc64":"9712795839E5BE01","md5":"907B3D8ABB4931A7F4205F4572537B7A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Region":1,"Coiled coil":1,"Motif":1,"Compositional bias":2,"Active site":1},"uniParcId":"UPI000162F4C2"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B0B0K9","uniProtkbId":"B0B0K9_STAIN","entryAudit":{"firstPublicDate":"2008-02-26","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-02-26","entryVersion":97,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18887.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"value":"DNA topoisomerase 4 subunit B"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"value":"5.6.2.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"value":"Topoisomerase IV subunit B"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18887.1"}],"value":"grlB"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"value":"parE"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"value":"Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP-dependent breakage, passage and rejoining of double-stranded DNA.","ecNumber":"5.6.2.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000185"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001946"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"value":"Heterotetramer composed of ParC and ParE"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00010708"}],"value":"Belongs to the type II topoisomerase GyrB family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"value":"Belongs to the type II topoisomerase family. ParE type 2 subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":398,"modifier":"EXACT"},"end":{"value":512,"modifier":"EXACT"}},"description":"Toprim","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50880"}]},{"type":"Region","location":{"start":{"value":361,"modifier":"EXACT"},"end":{"value":390,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":361,"modifier":"EXACT"},"end":{"value":372,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":315,"modifier":"EXACT"},"end":{"value":315,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Site","location":{"start":{"value":429,"modifier":"EXACT"},"end":{"value":429,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00995"}]},{"type":"Site","location":{"start":{"value":432,"modifier":"EXACT"},"end":{"value":432,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00995"}]},{"type":"Site","location":{"start":{"value":484,"modifier":"EXACT"},"end":{"value":484,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}]},{"type":"Site","location":{"start":{"value":600,"modifier":"EXACT"},"end":{"value":600,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023125"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022842"}],"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023029"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00939"}],"id":"KW-0799","category":"Molecular function","name":"Topoisomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"18305127","citationType":"journal article","authors":["Descloux S.","Rossano A.","Perreten V."],"citationCrossReferences":[{"database":"PubMed","id":"18305127"},{"database":"DOI","id":"10.1128/JCM.02255-07"}],"title":"Characterization of new staphylococcal cassette chromosome mec (SCCmec) and topoisomerase genes in fluoroquinolone- and methicillin-resistant Staphylococcus pseudintermedius.","publicationDate":"2008","journal":"J. Clin. Microbiol.","firstPage":"1818","lastPage":"1823","volume":"46"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18887.1"}],"value":"DSM20373","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18887.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AM262970","properties":[{"key":"ProteinId","value":"CAK18887.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B0B0K9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005694","properties":[{"key":"GoTerm","value":"C:chromosome"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0034335","properties":[{"key":"GoTerm","value":"F:DNA negative supercoiling activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0007059","properties":[{"key":"GoTerm","value":"P:chromosome segregation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006265","properties":[{"key":"GoTerm","value":"P:DNA topological change"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd16928","properties":[{"key":"EntryName","value":"HATPase_GyrB-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00822","properties":[{"key":"EntryName","value":"TopoII_Trans_DNA_gyrase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.230.10:FF:000005","properties":[{"key":"EntryName","value":"DNA gyrase subunit B"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.670:FF:000002","properties":[{"key":"EntryName","value":"DNA gyrase subunit B"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.670","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.565.10","properties":[{"key":"EntryName","value":"Histidine kinase-like ATPase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00939","properties":[{"key":"EntryName","value":"ParE_type2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002288","properties":[{"key":"EntryName","value":"DNA_gyrase_B_C"}]},{"database":"InterPro","id":"IPR036890","properties":[{"key":"EntryName","value":"HATPase_C_sf"}]},{"database":"InterPro","id":"IPR003594","properties":[{"key":"EntryName","value":"HATPase_dom"}]},{"database":"InterPro","id":"IPR005740","properties":[{"key":"EntryName","value":"ParE_type2"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR014721","properties":[{"key":"EntryName","value":"Ribsml_uS5_D2-typ_fold_subgr"}]},{"database":"InterPro","id":"IPR001241","properties":[{"key":"EntryName","value":"Topo_IIA"}]},{"database":"InterPro","id":"IPR013760","properties":[{"key":"EntryName","value":"Topo_IIA-like_dom_sf"}]},{"database":"InterPro","id":"IPR000565","properties":[{"key":"EntryName","value":"Topo_IIA_B"}]},{"database":"InterPro","id":"IPR013759","properties":[{"key":"EntryName","value":"Topo_IIA_B_C"}]},{"database":"InterPro","id":"IPR013506","properties":[{"key":"EntryName","value":"Topo_IIA_bsu_dom2"}]},{"database":"InterPro","id":"IPR018522","properties":[{"key":"EntryName","value":"TopoIIA_CS"}]},{"database":"InterPro","id":"IPR006171","properties":[{"key":"EntryName","value":"TOPRIM_dom"}]},{"database":"NCBIfam","id":"TIGR01058","properties":[{"key":"EntryName","value":"parE_Gpos"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF004189","properties":[{"key":"EntryName","value":"PRK05644.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45866","properties":[{"key":"EntryName","value":"DNA GYRASE/TOPOISOMERASE SUBUNIT B"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45866:SF12","properties":[{"key":"EntryName","value":"DNA TOPOISOMERASE 4 SUBUNIT B"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00204","properties":[{"key":"EntryName","value":"DNA_gyraseB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00986","properties":[{"key":"EntryName","value":"DNA_gyraseB_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02518","properties":[{"key":"EntryName","value":"HATPase_c"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01751","properties":[{"key":"EntryName","value":"Toprim"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01159","properties":[{"key":"EntryName","value":"DNAGYRASEB"}]},{"database":"PRINTS","id":"PR00418","properties":[{"key":"EntryName","value":"TPI2FAMILY"}]},{"database":"SMART","id":"SM00387","properties":[{"key":"EntryName","value":"HATPase_c"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00433","properties":[{"key":"EntryName","value":"TOP2c"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55874","properties":[{"key":"EntryName","value":"ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56719","properties":[{"key":"EntryName","value":"Type II DNA topoisomerase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00177","properties":[{"key":"EntryName","value":"TOPOISOMERASE_II"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50880","properties":[{"key":"EntryName","value":"TOPRIM"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MYIGSTDKRGLHHLVYEVVDNSVDEILNGYGNEINVTINEDESITVADNGRGMPTGTHRSGKPTVEVIFTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSESLTVEIHRDGEIFEQQFAHGGVPQTKLIKKGKTKRTGTTVTFKPDPEIFKAATSFNFETLSERLQESAFLLQNLKITLEDRRAGKERSEVYHYEEGIKAFVSYVNEGKEVLHDVALFQGTSNEIEVDVSFQYNDQYSESIMSFVNNVRTKDGGTHEVGFKTAMTRVFNEYARRIGDLKAKDKNLEGNDIREGLTAIISVRIPEHLLQFEGQTKSKLGTPEARSAVDAIVAEKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKAREDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGNDFNLEDSNYNRIIIMTDADTDGAHIQVLLLTFFFKYMRPLVMAGRVYIALPPLYKLEKGKGKNKKIAYAWTDEELEKLQREMGKGFVLQRYKGLGEMNPDQLWETTMNPETRTLIRVQIDDEVRSSQRVSTLMGDKVAPRREWIERHVQFGMQEDLSILENDEIQILSDEDIVEEDA","length":637,"molWeight":71432,"crc64":"8B2D4CBE689D41E4","md5":"7126E62201248C5A33E480B7A4E9DB17"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBUNIT":1,"SIMILARITY":2,"CAUTION":1},"countByFeatureType":{"Domain":1,"Region":1,"Compositional bias":1,"Binding site":4,"Site":4},"uniParcId":"UPI000162F4EE"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"B0B0L0","uniProtkbId":"B0B0L0_STAIN","entryAudit":{"firstPublicDate":"2008-02-26","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2008-02-26","entryVersion":84,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18888.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"value":"DNA topoisomerase 4 subunit A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"value":"5.6.2.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"value":"Topoisomerase IV subunit A"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18888.1"}],"value":"grlA"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"value":"parC"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"value":"Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP-dependent breakage, passage and rejoining of double-stranded DNA.","ecNumber":"5.6.2.2","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00000185"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01384"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00063644"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"value":"Heterotetramer composed of ParC and ParE"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"value":"Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":495,"modifier":"EXACT"}},"description":"Topo IIA-type catalytic","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52040"}]},{"type":"Coiled coil","location":{"start":{"value":426,"modifier":"EXACT"},"end":{"value":474,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Coils"}]},{"type":"Active site","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01384"}]},{"type":"Site","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}]},{"type":"Site","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}]},{"type":"Site","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}]},{"type":"Site","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}]},{"type":"Site","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"Interaction with DNA","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}]},{"type":"Site","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022475"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Coils"}],"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023125"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023235"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023029"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00937"}],"id":"KW-0799","category":"Molecular function","name":"Topoisomerase"}],"references":[{"referenceNumber":1,"citation":{"id":"18305127","citationType":"journal article","authors":["Descloux S.","Rossano A.","Perreten V."],"citationCrossReferences":[{"database":"PubMed","id":"18305127"},{"database":"DOI","id":"10.1128/JCM.02255-07"}],"title":"Characterization of new staphylococcal cassette chromosome mec (SCCmec) and topoisomerase genes in fluoroquinolone- and methicillin-resistant Staphylococcus pseudintermedius.","publicationDate":"2008","journal":"J. Clin. Microbiol.","firstPage":"1818","lastPage":"1823","volume":"46"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18888.1"}],"value":"DSM20373","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAK18888.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AM262970","properties":[{"key":"ProteinId","value":"CAK18888.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_019167736.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_JBBLTU010000001.1"}]},{"database":"AlphaFoldDB","id":"B0B0L0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"B0B0L0","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0188","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"GO","id":"GO:0005694","properties":[{"key":"GoTerm","value":"C:chromosome"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0009330","properties":[{"key":"GoTerm","value":"C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0019897","properties":[{"key":"GoTerm","value":"C:extrinsic component of plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0034335","properties":[{"key":"GoTerm","value":"F:DNA negative supercoiling activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0007059","properties":[{"key":"GoTerm","value":"P:chromosome segregation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006265","properties":[{"key":"GoTerm","value":"P:DNA topological change"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00187","properties":[{"key":"EntryName","value":"TOP4c"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.268.10:FF:000001","properties":[{"key":"EntryName","value":"DNA gyrase subunit A"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.199.10:FF:000001","properties":[{"key":"EntryName","value":"DNA gyrase subunit A"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.120.10.90:FF:000005","properties":[{"key":"EntryName","value":"DNA topoisomerase 4 subunit A"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1360.40","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.120.10.90","properties":[{"key":"EntryName","value":"DNA gyrase/topoisomerase IV, subunit A, C-terminal"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.199.10","properties":[{"key":"EntryName","value":"Topoisomerase II, domain 5"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.268.10","properties":[{"key":"EntryName","value":"Topoisomerase, domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00937","properties":[{"key":"EntryName","value":"ParC_type2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006691","properties":[{"key":"EntryName","value":"GyrA/parC_rep"}]},{"database":"InterPro","id":"IPR035516","properties":[{"key":"EntryName","value":"Gyrase/topoIV_suA_C"}]},{"database":"InterPro","id":"IPR013760","properties":[{"key":"EntryName","value":"Topo_IIA-like_dom_sf"}]},{"database":"InterPro","id":"IPR013758","properties":[{"key":"EntryName","value":"Topo_IIA_A/C_ab"}]},{"database":"InterPro","id":"IPR013757","properties":[{"key":"EntryName","value":"Topo_IIA_A_a_sf"}]},{"database":"InterPro","id":"IPR002205","properties":[{"key":"EntryName","value":"Topo_IIA_dom_A"}]},{"database":"InterPro","id":"IPR005741","properties":[{"key":"EntryName","value":"TopoIV_A_Gpos"}]},{"database":"InterPro","id":"IPR050220","properties":[{"key":"EntryName","value":"Type_II_DNA_Topoisomerases"}]},{"database":"NCBIfam","id":"TIGR01061","properties":[{"key":"EntryName","value":"parC_Gpos"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF004044","properties":[{"key":"EntryName","value":"PRK05561.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43493","properties":[{"key":"EntryName","value":"DNA GYRASE/TOPOISOMERASE SUBUNIT A"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43493:SF9","properties":[{"key":"EntryName","value":"DNA TOPOISOMERASE 4 SUBUNIT A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03989","properties":[{"key":"EntryName","value":"DNA_gyraseA_C"},{"key":"MatchStatus","value":"5"}]},{"database":"Pfam","id":"PF00521","properties":[{"key":"EntryName","value":"DNA_topoisoIV"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00434","properties":[{"key":"EntryName","value":"TOP4c"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF101904","properties":[{"key":"EntryName","value":"GyrA/ParC C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56719","properties":[{"key":"EntryName","value":"Type II DNA topoisomerase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52040","properties":[{"key":"EntryName","value":"TOPO_IIA"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAEIIQNLPLVDVIGDRFGRYSKYIIQERALPDVRDGLKPVQRRILYSMYSNGNTYDKHFRKSAKTVGDVIGQLHPHGDSSVYDAMVRLSQDWKLRHVLVEMHGNNGSIDNDPPAAMRYTEAKLSQLSEQLLRDIHKDTVDFVPNYDDTEMEPMVLPARFPNLLVNGSTGISAGYATDIPPHNLAEVITATLKYIDNPDLTVKQLMQYIKGPDFPTGGIVQGIDGIRKAYETGKGKVIVRAKADIETLRNGRSQIIVTEIPYEVNKSSLVKKIDELRADKKVEGIVEVRDETDRSGLRVAVELKKDVNATSILNFLYKNTDLQVAYNFNMVAITEGRPKLMGIKPIISSYLNHQIEVVTRRTSFDLKHTEERMHIVEGLMKALSILDEVIVLIRNSKNKRDAKNNLVATYDFTETQAEAIVMLQLYRLTNTDIVALQEEYDELKQKIAALKHILENHDALLNVIKDELSEIKKQFKQPRLSVIEEKISEIKIDKEVIVPSETVMLSLTQAGYIKRTSLRSFNASGKEEIGLKERDGVLKIIETNTQDYVMVFTNKGRYLLIPVHKLPDVKWKDLGQHVSQIVPIEEDERVVNCYTVSDFNDEHAIITATRQGMIKKSLLSGFKSTRTSKPLVAMKVKEGDALISVMYVPANAASQCITLLTHRGMSLTYPMSELPDTGLRAAGIKAINLKDEDHVVLADHIQPQDTILIATQRGALKRIGFKVLQQAKRAQRGITLLKELKKQPHRVVAARVCHPEQSQYRLVSQQDEVTGLIKDIHLSEQYTNGSFVVDTNTFGEIEDLFVD","length":803,"molWeight":90612,"crc64":"3A32ADE6CDEB26E9","md5":"CF7E3ED697764A4DCA77EB97235B693C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Coiled coil":1,"Active site":1,"Site":6},"uniParcId":"UPI000162F4EF"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q2LFA4","uniProtkbId":"Q2LFA4_STAIN","entryAudit":{"firstPublicDate":"2006-02-21","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2006-02-21","entryVersion":85,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69188.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}],"value":"Glyceraldehyde-3-phosphate dehydrogenase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}],"value":"1.2.1.-"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69188.1"}],"value":"gap"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003501"}],"value":"Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10300"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57604"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:59776"}],"ecNumber":"1.2.1.12","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047698"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10009"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004869"}],"value":"Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011881"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007406"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000397"}],"value":"Belongs to the glyceraldehyde-3-phosphate dehydrogenase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding","evidences":[{"evidenceCode":"ECO:0000259","source":"SMART","id":"SM00846"}]},{"type":"Active site","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-1"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10009"}]},{"type":"Binding site","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":5,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-2"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":173,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-2"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":203,"modifier":"EXACT"},"end":{"value":204,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-2"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":226,"modifier":"EXACT"},"end":{"value":226,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-2"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":307,"modifier":"EXACT"},"end":{"value":307,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Site","location":{"start":{"value":170,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"Activates thiol group during catalysis","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-4"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69188.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":310,"modifier":"EXACT"},"end":{"value":310,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69188.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023152"}],"id":"KW-0324","category":"Biological process","name":"Glycolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023027"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"id":"KW-0520","category":"Ligand","name":"NAD"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR000149-3"}],"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361160"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"16891498","citationType":"journal article","authors":["Layer F.","Ghebremedhin B.","Moder K.A.","Konig W.","Konig B."],"citationCrossReferences":[{"database":"PubMed","id":"16891498"},{"database":"DOI","id":"10.1128/JCM.00226-06"}],"title":"Comparative study using various methods for identification of Staphylococcus species in clinical specimens.","publicationDate":"2006","journal":"J. Clin. Microbiol.","firstPage":"2824","lastPage":"2830","volume":"44"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69188.1"}],"value":"DSM 20373","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69188.1"}]},{"referenceNumber":2,"citation":{"id":"17681623","citationType":"journal article","authors":["Layer F.","Ghebremedhin B.","Konig W.","Konig B."],"citationCrossReferences":[{"database":"PubMed","id":"17681623"},{"database":"DOI","id":"10.1016/j.mimet.2007.06.015"}],"title":"Differentiation of Staphylococcus spp. by terminal-restriction fragment length polymorphism analysis of glyceraldehyde-3-phosphate dehydrogenase-encoding gene.","publicationDate":"2007","journal":"J. Microbiol. Methods","firstPage":"542","lastPage":"549","volume":"70"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69188.1"}],"value":"DSM 20373","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABC69188.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DQ321690","properties":[{"key":"ProteinId","value":"ABC69188.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q2LFA4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0004365","properties":[{"key":"GoTerm","value":"F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0051287","properties":[{"key":"GoTerm","value":"F:NAD binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0050661","properties":[{"key":"GoTerm","value":"F:NADP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006006","properties":[{"key":"GoTerm","value":"P:glucose metabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd18126","properties":[{"key":"EntryName","value":"GAPDH_I_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd05214","properties":[{"key":"EntryName","value":"GAPDH_I_N"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.360.10:FF:000002","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.720:FF:000001","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.360.10","properties":[{"key":"EntryName","value":"Dihydrodipicolinate Reductase, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR020831","properties":[{"key":"EntryName","value":"GlycerAld/Erythrose_P_DH"}]},{"database":"InterPro","id":"IPR020830","properties":[{"key":"EntryName","value":"GlycerAld_3-P_DH_AS"}]},{"database":"InterPro","id":"IPR020829","properties":[{"key":"EntryName","value":"GlycerAld_3-P_DH_cat"}]},{"database":"InterPro","id":"IPR020828","properties":[{"key":"EntryName","value":"GlycerAld_3-P_DH_NAD(P)-bd"}]},{"database":"InterPro","id":"IPR006424","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-P_DH_1"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"NCBIfam","id":"TIGR01534","properties":[{"key":"EntryName","value":"GAPDH-I"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43148","properties":[{"key":"EntryName","value":"GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02800","properties":[{"key":"EntryName","value":"Gp_dh_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00044","properties":[{"key":"EntryName","value":"Gp_dh_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000149","properties":[{"key":"EntryName","value":"GAP_DH"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00078","properties":[{"key":"EntryName","value":"G3PDHDRGNASE"}]},{"database":"SMART","id":"SM00846","properties":[{"key":"EntryName","value":"Gp_dh_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55347","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00071","properties":[{"key":"EntryName","value":"GAPDH"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"GFGRIGRLAFRRIQDVENIEVVAVNDLTDDDMLAHLLKYDSTQGRFTEEVEVIDGGFRVNGKEVKSFSEPEPKNLPWGDLDIDVVLECTGFFTDKDKAEAHIEAGAKKVLISAPAKGDLKTIVYNVNHELLDGSETVVSGASCTTNSLAPVAKTLQDNFGIVEGLMTTIHAYTGDQNTLDAPHRKGDKRRARAAAENIIPNSTGAAKAIGLVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKEVSVEEVNKVMKEATNESFGYTEDEIVSSDVIGMTFGALFDATQTRVMTVGDRQLVKVAAWYDNEMS","length":310,"molWeight":33535,"crc64":"93F3510791F6D11D","md5":"9264A36EFCC2D5D499BC286446741221"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Active site":1,"Binding site":8,"Site":1,"Non-terminal residue":2},"uniParcId":"UPI0000683FE3"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A6BML2","uniProtkbId":"A6BML2_STAIN","entryAudit":{"firstPublicDate":"2007-07-24","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2007-07-24","entryVersion":57,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAF64524.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00016676"}],"value":"Thermonuclease"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011942"}],"value":"3.1.31.1"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00031238"}],"value":"Micrococcal nuclease"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00030535"}],"value":"Staphylococcal nuclease"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAF64524.1"}],"value":"nuc"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.","ecNumber":"3.1.31.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10049"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Ca(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001913"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29108"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00272"}],"value":"Belongs to the thermonuclease family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"TNase-like","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50830"}]},{"type":"Active site","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10048"}]},{"type":"Active site","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10048"}]},{"type":"Active site","location":{"start":{"value":114,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10049"}]},{"type":"Binding site","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00272"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108"}},{"type":"Binding site","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00272"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108"}},{"type":"Binding site","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00272"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022837"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00272"}],"id":"KW-0106","category":"Ligand","name":"Calcium"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022759"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00272"}],"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00272"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00272"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022722"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00272"}],"id":"KW-0540","category":"Molecular function","name":"Nuclease"}],"references":[{"referenceNumber":1,"citation":{"id":"17596353","citationType":"journal article","authors":["Sasaki T.","Kikuchi K.","Tanaka Y.","Takahashi N.","Kamata S.","Hiramatsu K."],"citationCrossReferences":[{"database":"PubMed","id":"17596353"},{"database":"DOI","id":"10.1128/JCM.00360-07"}],"title":"Reclassification of phenotypically identified staphylococcus intermedius strains.","publicationDate":"2007","journal":"J. Clin. Microbiol.","firstPage":"2770","lastPage":"2778","volume":"45"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAF64524.1"}],"value":"ATCC 29663","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"BAF64524.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB327165","properties":[{"key":"ProteinId","value":"BAF64524.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_019167764.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_JBBLTU010000001.1"}]},{"database":"AlphaFoldDB","id":"A6BML2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:1990599","properties":[{"key":"GoTerm","value":"F:3' overhang single-stranded DNA endonuclease activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0005509","properties":[{"key":"GoTerm","value":"F:calcium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003676","properties":[{"key":"GoTerm","value":"F:nucleic acid binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00175","properties":[{"key":"EntryName","value":"SNc"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.90","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR035437","properties":[{"key":"EntryName","value":"SNase_OB-fold_sf"}]},{"database":"InterPro","id":"IPR016071","properties":[{"key":"EntryName","value":"Staphylococal_nuclease_OB-fold"}]},{"database":"InterPro","id":"IPR002071","properties":[{"key":"EntryName","value":"Thermonucl_AS"}]},{"database":"NCBIfam","id":"NF047694","properties":[{"key":"EntryName","value":"TnucaseNucIStaph"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12302","properties":[{"key":"EntryName","value":"EBNA2 BINDING PROTEIN P100"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12302:SF3","properties":[{"key":"EntryName","value":"SERINE_THREONINE-PROTEIN KINASE 31"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00565","properties":[{"key":"EntryName","value":"SNase"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00318","properties":[{"key":"EntryName","value":"SNc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50199","properties":[{"key":"EntryName","value":"Staphylococcal nuclease"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01123","properties":[{"key":"EntryName","value":"TNASE_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01284","properties":[{"key":"EntryName","value":"TNASE_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50830","properties":[{"key":"EntryName","value":"TNASE_3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKKITTGTLILVIAIIVLGFQYVNGDGPFKKSTTDVKGESYLVKRVIDGDTIIIDKDGQDERVRLIGVDTPETVKPNTPVQPYGKAASNFTKKHLTNQHVKLEYDREPKDKYGRTLAYVWLGDEMFNVKLAQEGLARAKFYPPNDKYRILIEQAQKEAQKKQLNIWER","length":168,"molWeight":19212,"crc64":"AF9982513D7DF9A0","md5":"9A39A1E945E6F08DA424A5E251C296D3"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Active site":3,"Binding site":3},"uniParcId":"UPI00015746CB"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A6MFN2","uniProtkbId":"A6MFN2_STAIN","entryAudit":{"firstPublicDate":"2007-07-24","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2007-07-24","entryVersion":64,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96850.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012865"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"Beta-lactamase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012865"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"3.5.2.6"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96850.1"}],"value":"blaZ"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a beta-lactam + H2O = a substituted beta-amino acid","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20401"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:35627"},{"database":"ChEBI","id":"CHEBI:140347"}],"ecNumber":"3.5.2.6","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10101"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009009"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"Belongs to the class-A beta-lactamase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":260,"modifier":"EXACT"}},"description":"Beta-lactamase class A catalytic","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF13354"}]},{"type":"Active site","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Acyl-ester intermediate","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10101"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96850.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96850.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"}],"references":[{"referenceNumber":1,"citation":{"id":"17350771","citationType":"journal article","authors":["Malik S.","Christensen H.","Peng H.","Barton M.D."],"citationCrossReferences":[{"database":"PubMed","id":"17350771"},{"database":"DOI","id":"10.1016/j.vetmic.2007.02.012"}],"title":"Presence and diversity of the beta-lactamase gene in cat and dog staphylococci.","publicationDate":"2007","journal":"Vet. Microbiol.","firstPage":"162","lastPage":"168","volume":"123"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96850.1"}],"value":"CS32","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96850.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DQ919082","properties":[{"key":"ProteinId","value":"ABK96850.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A6MFN2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0008800","properties":[{"key":"GoTerm","value":"F:beta-lactamase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0030655","properties":[{"key":"GoTerm","value":"P:beta-lactam antibiotic catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0046677","properties":[{"key":"GoTerm","value":"P:response to antibiotic"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"3.40.710.10","properties":[{"key":"EntryName","value":"DD-peptidase/beta-lactamase superfamily"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR012338","properties":[{"key":"EntryName","value":"Beta-lactam/transpept-like"}]},{"database":"InterPro","id":"IPR045155","properties":[{"key":"EntryName","value":"Beta-lactam_cat"}]},{"database":"InterPro","id":"IPR000871","properties":[{"key":"EntryName","value":"Beta-lactam_class-A"}]},{"database":"InterPro","id":"IPR023650","properties":[{"key":"EntryName","value":"Beta-lactam_class-A_AS"}]},{"database":"InterPro","id":"IPR058138","properties":[{"key":"EntryName","value":"BlaZ"}]},{"database":"NCBIfam","id":"NF033103","properties":[{"key":"EntryName","value":"bla_class_A"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033139","properties":[{"key":"EntryName","value":"blaZ"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033141","properties":[{"key":"EntryName","value":"blaZ_gen"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35333","properties":[{"key":"EntryName","value":"BETA-LACTAMASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35333:SF3","properties":[{"key":"EntryName","value":"BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13354","properties":[{"key":"EntryName","value":"Beta-lactamase2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00118","properties":[{"key":"EntryName","value":"BLACTAMASEA"}]},{"database":"SUPFAM","id":"SSF56601","properties":[{"key":"EntryName","value":"beta-lactamase/transpeptidase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00146","properties":[{"key":"EntryName","value":"BETA_LACTAMASE_A"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"IFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALDTKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPKDYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDK","length":264,"molWeight":29384,"crc64":"4B1AFB5FDA4D2D5E","md5":"17A97CD6CFCB1DEBB634AC320FEB8E88"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI0001578735"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A6MFN5","uniProtkbId":"A6MFN5_STAIN","entryAudit":{"firstPublicDate":"2007-07-24","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2007-07-24","entryVersion":69,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96853.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012865"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"Beta-lactamase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012865"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"3.5.2.6"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96853.1"}],"value":"blaZ"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a beta-lactam + H2O = a substituted beta-amino acid","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20401"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:35627"},{"database":"ChEBI","id":"CHEBI:140347"}],"ecNumber":"3.5.2.6","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10101"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009009"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"Belongs to the class-A beta-lactamase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"Beta-lactamase class A catalytic","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF13354"}]},{"type":"Active site","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Acyl-ester intermediate","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10101"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96853.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":261,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96853.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"}],"references":[{"referenceNumber":1,"citation":{"id":"17350771","citationType":"journal article","authors":["Malik S.","Christensen H.","Peng H.","Barton M.D."],"citationCrossReferences":[{"database":"PubMed","id":"17350771"},{"database":"DOI","id":"10.1016/j.vetmic.2007.02.012"}],"title":"Presence and diversity of the beta-lactamase gene in cat and dog staphylococci.","publicationDate":"2007","journal":"Vet. Microbiol.","firstPage":"162","lastPage":"168","volume":"123"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96859.1"}],"value":"FD755","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96853.1"}],"value":"KE","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96853.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DQ919085","properties":[{"key":"ProteinId","value":"ABK96853.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"DQ919091","properties":[{"key":"ProteinId","value":"ABK96859.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A6MFN5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0008800","properties":[{"key":"GoTerm","value":"F:beta-lactamase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0030655","properties":[{"key":"GoTerm","value":"P:beta-lactam antibiotic catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0046677","properties":[{"key":"GoTerm","value":"P:response to antibiotic"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"3.40.710.10","properties":[{"key":"EntryName","value":"DD-peptidase/beta-lactamase superfamily"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR012338","properties":[{"key":"EntryName","value":"Beta-lactam/transpept-like"}]},{"database":"InterPro","id":"IPR045155","properties":[{"key":"EntryName","value":"Beta-lactam_cat"}]},{"database":"InterPro","id":"IPR000871","properties":[{"key":"EntryName","value":"Beta-lactam_class-A"}]},{"database":"InterPro","id":"IPR023650","properties":[{"key":"EntryName","value":"Beta-lactam_class-A_AS"}]},{"database":"NCBIfam","id":"NF033103","properties":[{"key":"EntryName","value":"bla_class_A"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35333","properties":[{"key":"EntryName","value":"BETA-LACTAMASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35333:SF3","properties":[{"key":"EntryName","value":"BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13354","properties":[{"key":"EntryName","value":"Beta-lactamase2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00118","properties":[{"key":"EntryName","value":"BLACTAMASEA"}]},{"database":"SUPFAM","id":"SSF56601","properties":[{"key":"EntryName","value":"beta-lactamase/transpeptidase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00146","properties":[{"key":"EntryName","value":"BETA_LACTAMASE_A"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"IAIALVLSACNSTSSHAKELNNLEKKYNANIGVYALDTKSGKEVKFNADKRFAYASTSKAINSAILLEQAPYNKLNKKVHINKDDIVAYSPILEKYVGKDITLKELIEASMKYSDNTANNKIINEIGGIKKIKKRLKKLGDKVTNPVRYEIELNYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMLNNKNGDTLIKDGVPKDYKVADKSGQAITYASRNDVAFIYPKNQSEPIILVIFTNKDNKSDKPNDK","length":261,"molWeight":29083,"crc64":"651E0563D6B13DBB","md5":"B419833B1ECD17DA049D5A2336B282B9"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI0001578738"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A6MFN9","uniProtkbId":"A6MFN9_STAIN","entryAudit":{"firstPublicDate":"2007-07-24","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2007-07-24","entryVersion":63,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96857.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012865"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"Beta-lactamase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012865"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"3.5.2.6"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96857.1"}],"value":"blaZ"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a beta-lactam + H2O = a substituted beta-amino acid","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20401"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:35627"},{"database":"ChEBI","id":"CHEBI:140347"}],"ecNumber":"3.5.2.6","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10101"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009009"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"Belongs to the class-A beta-lactamase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":262,"modifier":"EXACT"}},"description":"Beta-lactamase class A catalytic","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF13354"}]},{"type":"Active site","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"Acyl-ester intermediate","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10101"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96857.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":266,"modifier":"EXACT"},"end":{"value":266,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96857.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"}],"references":[{"referenceNumber":1,"citation":{"id":"17350771","citationType":"journal article","authors":["Malik S.","Christensen H.","Peng H.","Barton M.D."],"citationCrossReferences":[{"database":"PubMed","id":"17350771"},{"database":"DOI","id":"10.1016/j.vetmic.2007.02.012"}],"title":"Presence and diversity of the beta-lactamase gene in cat and dog staphylococci.","publicationDate":"2007","journal":"Vet. Microbiol.","firstPage":"162","lastPage":"168","volume":"123"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96857.1"}],"value":"FD733","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96857.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DQ919089","properties":[{"key":"ProteinId","value":"ABK96857.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A6MFN9","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0008800","properties":[{"key":"GoTerm","value":"F:beta-lactamase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0030655","properties":[{"key":"GoTerm","value":"P:beta-lactam antibiotic catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0046677","properties":[{"key":"GoTerm","value":"P:response to antibiotic"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"3.40.710.10","properties":[{"key":"EntryName","value":"DD-peptidase/beta-lactamase superfamily"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR012338","properties":[{"key":"EntryName","value":"Beta-lactam/transpept-like"}]},{"database":"InterPro","id":"IPR045155","properties":[{"key":"EntryName","value":"Beta-lactam_cat"}]},{"database":"InterPro","id":"IPR000871","properties":[{"key":"EntryName","value":"Beta-lactam_class-A"}]},{"database":"InterPro","id":"IPR023650","properties":[{"key":"EntryName","value":"Beta-lactam_class-A_AS"}]},{"database":"InterPro","id":"IPR058138","properties":[{"key":"EntryName","value":"BlaZ"}]},{"database":"NCBIfam","id":"NF033103","properties":[{"key":"EntryName","value":"bla_class_A"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033139","properties":[{"key":"EntryName","value":"blaZ"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF033141","properties":[{"key":"EntryName","value":"blaZ_gen"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35333","properties":[{"key":"EntryName","value":"BETA-LACTAMASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35333:SF3","properties":[{"key":"EntryName","value":"BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13354","properties":[{"key":"EntryName","value":"Beta-lactamase2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00118","properties":[{"key":"EntryName","value":"BLACTAMASEA"}]},{"database":"SUPFAM","id":"SSF56601","properties":[{"key":"EntryName","value":"beta-lactamase/transpeptidase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00146","properties":[{"key":"EntryName","value":"BETA_LACTAMASE_A"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"KLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALDTKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYVGKVITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPKDYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDK","length":266,"molWeight":29609,"crc64":"DB604FF806E7C057","md5":"31C43BD07262D4160BB9DC5DDE3F51F3"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI000157873A"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A6MFP0","uniProtkbId":"A6MFP0_STAIN","entryAudit":{"firstPublicDate":"2007-07-24","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2007-07-24","entryVersion":61,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96858.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012865"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"Beta-lactamase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012865"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"3.5.2.6"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96858.1"}],"value":"blaZ"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a beta-lactam + H2O = a substituted beta-amino acid","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20401"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:35627"},{"database":"ChEBI","id":"CHEBI:140347"}],"ecNumber":"3.5.2.6","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10101"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00009009"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"value":"Belongs to the class-A beta-lactamase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"Beta-lactamase class A catalytic","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF13354"}]},{"type":"Active site","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Acyl-ester intermediate","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10101"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96858.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":260,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96858.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023251"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU361140"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"}],"references":[{"referenceNumber":1,"citation":{"id":"17350771","citationType":"journal article","authors":["Malik S.","Christensen H.","Peng H.","Barton M.D."],"citationCrossReferences":[{"database":"PubMed","id":"17350771"},{"database":"DOI","id":"10.1016/j.vetmic.2007.02.012"}],"title":"Presence and diversity of the beta-lactamase gene in cat and dog staphylococci.","publicationDate":"2007","journal":"Vet. Microbiol.","firstPage":"162","lastPage":"168","volume":"123"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96858.1"}],"value":"FD734","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ABK96858.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DQ919090","properties":[{"key":"ProteinId","value":"ABK96858.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A6MFP0","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0008800","properties":[{"key":"GoTerm","value":"F:beta-lactamase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0030655","properties":[{"key":"GoTerm","value":"P:beta-lactam antibiotic catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0046677","properties":[{"key":"GoTerm","value":"P:response to antibiotic"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"3.40.710.10","properties":[{"key":"EntryName","value":"DD-peptidase/beta-lactamase superfamily"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR012338","properties":[{"key":"EntryName","value":"Beta-lactam/transpept-like"}]},{"database":"InterPro","id":"IPR045155","properties":[{"key":"EntryName","value":"Beta-lactam_cat"}]},{"database":"InterPro","id":"IPR000871","properties":[{"key":"EntryName","value":"Beta-lactam_class-A"}]},{"database":"InterPro","id":"IPR023650","properties":[{"key":"EntryName","value":"Beta-lactam_class-A_AS"}]},{"database":"NCBIfam","id":"NF033103","properties":[{"key":"EntryName","value":"bla_class_A"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35333","properties":[{"key":"EntryName","value":"BETA-LACTAMASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35333:SF3","properties":[{"key":"EntryName","value":"BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13354","properties":[{"key":"EntryName","value":"Beta-lactamase2"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00118","properties":[{"key":"EntryName","value":"BLACTAMASEA"}]},{"database":"SUPFAM","id":"SSF56601","properties":[{"key":"EntryName","value":"beta-lactamase/transpeptidase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00146","properties":[{"key":"EntryName","value":"BETA_LACTAMASE_A"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"VIALVLSACNSNSSHTQELNDLEKKYNAHIGVYALDTKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQHLKELGDKVTNPVRYEIELNYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPKDYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDK","length":260,"molWeight":29012,"crc64":"045970A4AE57E21D","md5":"31175866108475C3DB8936A270A35DBB"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Active site":1,"Non-terminal residue":2},"uniParcId":"UPI000157873B"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"E5KY56","uniProtkbId":"E5KY56_STAIN","entryAudit":{"firstPublicDate":"2011-02-08","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2011-02-08","entryVersion":38,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADR80663.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012418"}],"value":"DNA-directed RNA polymerase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012418"}],"value":"2.7.7.6"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADR80663.1"}],"value":"rpoB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000434"}],"value":"Belongs to the RNA polymerase beta chain family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"RNA polymerase Rpb2","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04565"}]},{"type":"Domain","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":142,"modifier":"EXACT"}},"description":"DNA-directed RNA polymerase beta subunit external 1","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF10385"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADR80663.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":151,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADR80663.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022478"}],"id":"KW-0240","category":"Cellular component","name":"DNA-directed RNA polymerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022695"}],"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023163"}],"id":"KW-0804","category":"Biological process","name":"Transcription"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"21095086","citationType":"journal article","authors":["Svec P.","Pantucek R.","Petras P.","Sedlacek I.","Novakova D."],"citationCrossReferences":[{"database":"PubMed","id":"21095086"},{"database":"DOI","id":"10.1016/j.syapm.2010.09.004"}],"title":"Identification of Staphylococcus spp. using (GTG)?-PCR fingerprinting.","publicationDate":"2010","journal":"Syst. Appl. Microbiol.","firstPage":"451","lastPage":"456","volume":"33"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADR80663.1"}],"value":"CCM 7843","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADR80663.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"HQ259717","properties":[{"key":"ProteinId","value":"ADR80663.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"E5KY56","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003899","properties":[{"key":"GoTerm","value":"F:DNA-directed RNA polymerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0032549","properties":[{"key":"GoTerm","value":"F:ribonucleoside binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006351","properties":[{"key":"GoTerm","value":"P:DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.90.1100.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR019462","properties":[{"key":"EntryName","value":"DNA-dir_RNA_pol_bsu_external_1"}]},{"database":"InterPro","id":"IPR015712","properties":[{"key":"EntryName","value":"DNA-dir_RNA_pol_su2"}]},{"database":"InterPro","id":"IPR007645","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_3"}]},{"database":"PANTHER","id":"PTHR20856","properties":[{"key":"EntryName","value":"DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04565","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF10385","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_45"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF64484","properties":[{"key":"EntryName","value":"beta and beta-prime subunits of DNA dependent RNA-polymerase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"QANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYSHYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDIETNTITDQIDYLTADEEDSYVVAQANSRLDENGRFIDDEIVCRFRGNNTTMAKEKMDYMDVSPKQVVSAAT","length":151,"molWeight":17049,"crc64":"355AE6A72A365ACB","md5":"D140DBB813A486DB213F2897401EA895"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Non-terminal residue":2},"uniParcId":"UPI0001DC1878"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"F6JWB4","uniProtkbId":"F6JWB4_STAIN","entryAudit":{"firstPublicDate":"2011-07-27","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-07-27","entryVersion":33,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADG86215.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012682"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"value":"Superoxide dismutase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012682"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"value":"1.15.1.1"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADG86215.1"}],"value":"sodA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"value":"Destroys radicals which are normally produced within the cells and which are toxic to biological systems"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 superoxide + 2 H(+) = H2O2 + O2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20696"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16240"},{"database":"ChEBI","id":"CHEBI:18421"}],"ecNumber":"1.15.1.1","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001936"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008714"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"value":"Belongs to the iron/manganese superoxide dismutase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"Manganese/iron superoxide dismutase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00081"}]},{"type":"Domain","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"Manganese/iron superoxide dismutase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02777"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADG86215.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADG86215.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023211"}],"id":"KW-0464","category":"Ligand","name":"Manganese"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"21586304","citationType":"journal article","authors":["Chanchaithong P.","Prapasarakul N."],"citationCrossReferences":[{"database":"PubMed","id":"21586304"},{"database":"DOI","id":"10.1016/j.mimet.2011.04.019"}],"title":"Biochemical markers and protein pattern analysis for canine coagulase-positive staphylococci and their distribution on dog skin.","publicationDate":"2011","journal":"J. Microbiol. Methods","firstPage":"175","lastPage":"181","volume":"86"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADG86215.1"}],"value":"CVMP0309","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"ADG86215.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"GU930734","properties":[{"key":"ProteinId","value":"ADG86215.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"F6JWB4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004784","properties":[{"key":"GoTerm","value":"F:superoxide dismutase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"FunFam","id":"1.10.287.990:FF:000001","properties":[{"key":"EntryName","value":"Superoxide dismutase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.287.990","properties":[{"key":"EntryName","value":"Fe,Mn superoxide dismutase (SOD) domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.55.40.20","properties":[{"key":"EntryName","value":"Iron/manganese superoxide dismutase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001189","properties":[{"key":"EntryName","value":"Mn/Fe_SOD"}]},{"database":"InterPro","id":"IPR019832","properties":[{"key":"EntryName","value":"Mn/Fe_SOD_C"}]},{"database":"InterPro","id":"IPR019831","properties":[{"key":"EntryName","value":"Mn/Fe_SOD_N"}]},{"database":"InterPro","id":"IPR036324","properties":[{"key":"EntryName","value":"Mn/Fe_SOD_N_sf"}]},{"database":"InterPro","id":"IPR036314","properties":[{"key":"EntryName","value":"SOD_C_sf"}]},{"database":"PANTHER","id":"PTHR43595","properties":[{"key":"EntryName","value":"37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43595:SF2","properties":[{"key":"EntryName","value":"SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02777","properties":[{"key":"EntryName","value":"Sod_Fe_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00081","properties":[{"key":"EntryName","value":"Sod_Fe_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01703","properties":[{"key":"EntryName","value":"MNSODISMTASE"}]},{"database":"SUPFAM","id":"SSF54719","properties":[{"key":"EntryName","value":"Fe,Mn superoxide dismutase (SOD), C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF46609","properties":[{"key":"EntryName","value":"Fe,Mn superoxide dismutase (SOD), N-terminal domain"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"DKETMEIHHSKHHNTYVTKLNAAVEGTEFENKSLEDLIANLNSVPENIRTAVRNNGGGHLNHSIFWQLLTPNSEEKGEVVDKIKEQWGSLDEFKNEFADKAAARFGSGWAWLVVNNGKLEIVTTANQDSPLTDGLSP","length":137,"molWeight":15254,"crc64":"007ED08C2273F987","md5":"AFE244278E97F2661E540BBB5D1CBC55"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Non-terminal residue":2},"uniParcId":"UPI00020CB7DD"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q56CZ5","uniProtkbId":"Q56CZ5_STAIN","entryAudit":{"firstPublicDate":"2005-05-10","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2005-05-10","entryVersion":82,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78521.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"4: Predicted","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78521.1"}],"value":"AgrA"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78521.1"}],"value":"agrA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00037164"}],"value":"Required for high-level post-exponential phase expression of a series of secreted proteins"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004496"}],"value":"Cytoplasm","id":"SL-0086"}}]}],"features":[{"type":"Domain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"Response regulatory","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50110"}]},{"type":"Domain","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"HTH LytTR-type","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS50930"}]},{"type":"Modified residue","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"4-aspartylphosphate","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00169"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023159"}],"id":"KW-0010","category":"Molecular function","name":"Activator"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022490"}],"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023125"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00112"}],"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022553"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00169"}],"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023163"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00112"}],"id":"KW-0804","category":"Biological process","name":"Transcription"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023015"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU00112"}],"id":"KW-0805","category":"Biological process","name":"Transcription regulation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023012"}],"id":"KW-0902","category":"Biological process","name":"Two-component regulatory system"}],"references":[{"referenceNumber":1,"citation":{"id":"16622233","citationType":"journal article","authors":["Sung J.M.","Chantler P.D.","Lloyd D.H."],"citationCrossReferences":[{"database":"PubMed","id":"16622233"},{"database":"DOI","id":"10.1128/IAI.74.5.2947-2956.2006"}],"title":"Accessory gene regulator locus of Staphylococcus intermedius.","publicationDate":"2006","journal":"Infect. Immun.","firstPage":"2947","lastPage":"2956","volume":"74"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78521.1"}],"value":"S3","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78521.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY965912","properties":[{"key":"ProteinId","value":"AAX78521.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q56CZ5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000156","properties":[{"key":"GoTerm","value":"F:phosphorelay response regulator activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006351","properties":[{"key":"GoTerm","value":"P:DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006355","properties":[{"key":"GoTerm","value":"P:regulation of DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd17533","properties":[{"key":"EntryName","value":"REC_LytTR_AgrA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.2300","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.1020","properties":[{"key":"EntryName","value":"LytTr DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR011006","properties":[{"key":"EntryName","value":"CheY-like_sf"}]},{"database":"InterPro","id":"IPR046947","properties":[{"key":"EntryName","value":"LytR-like"}]},{"database":"InterPro","id":"IPR007492","properties":[{"key":"EntryName","value":"LytTR_DNA-bd_dom"}]},{"database":"InterPro","id":"IPR001789","properties":[{"key":"EntryName","value":"Sig_transdc_resp-reg_receiver"}]},{"database":"NCBIfam","id":"NF046049","properties":[{"key":"EntryName","value":"quorum_RR_AgrA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37299:SF3","properties":[{"key":"EntryName","value":"STAGE 0 SPORULATION PROTEIN A HOMOLOG"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37299","properties":[{"key":"EntryName","value":"TRANSCRIPTIONAL REGULATOR-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04397","properties":[{"key":"EntryName","value":"LytTR"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00072","properties":[{"key":"EntryName","value":"Response_reg"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00850","properties":[{"key":"EntryName","value":"LytTR"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00448","properties":[{"key":"EntryName","value":"REC"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52172","properties":[{"key":"EntryName","value":"CheY-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50930","properties":[{"key":"EntryName","value":"HTH_LYTTR"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50110","properties":[{"key":"EntryName","value":"RESPONSE_REGULATORY"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKILICEDDPKQRERMVSIIENYIMIEEKPMKIELATNDPYAILETSKNMTDIGCYFLDIQLESDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKVAAMDFIFKDDPEELRTRIIDCLETALKRLDLLTKDHTVETLELKRGTSSVYVNYDDVMFFESSPKSHRLIAHLDNRQIEFYGNLKELAQLDDRFFRCHNSYVLNRRNITSVDTKERIAYFKNEEFCYVSVRNLKKI","length":238,"molWeight":28002,"crc64":"44570419F2E4C172","md5":"92BB7B844AC5A29AF1F51C146E3D1F67"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1},"countByFeatureType":{"Domain":2,"Modified residue":1},"uniParcId":"UPI0000504D73"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q56CZ8","uniProtkbId":"Q56CZ8_STAIN","entryAudit":{"firstPublicDate":"2005-05-10","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2005-05-10","entryVersion":43,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78518.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Accessory gene regulator protein B"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"value":"3.4.-.-"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"},{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78518.1"}],"value":"agrB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Essential for the production of a quorum sensing system signal molecule, the autoinducing peptide (AIP). This quorum sensing system is responsible for the regulation of the expression of virulence factor genes. Involved in the proteolytic processing of AgrD, the precursor of AIP"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"value":"Belongs to the AgrB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transmembrane","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}]},{"type":"Transmembrane","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}]},{"type":"Transmembrane","location":{"start":{"value":106,"modifier":"EXACT"},"end":{"value":128,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}]},{"type":"Transmembrane","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}]},{"type":"Transmembrane","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022475"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022654"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"id":"KW-0673","category":"Biological process","name":"Quorum sensing"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022989"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023026"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_00784"}],"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"16622233","citationType":"journal article","authors":["Sung J.M.","Chantler P.D.","Lloyd D.H."],"citationCrossReferences":[{"database":"PubMed","id":"16622233"},{"database":"DOI","id":"10.1128/IAI.74.5.2947-2956.2006"}],"title":"Accessory gene regulator locus of Staphylococcus intermedius.","publicationDate":"2006","journal":"Infect. Immun.","firstPage":"2947","lastPage":"2956","volume":"74"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78518.1"}],"value":"S3","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78518.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY965912","properties":[{"key":"ProteinId","value":"AAX78518.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q56CZ8","properties":[{"key":"Description","value":"-"}]},{"database":"MEROPS","id":"C75.001","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008233","properties":[{"key":"GoTerm","value":"F:peptidase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009372","properties":[{"key":"GoTerm","value":"P:quorum sensing"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_00784","properties":[{"key":"EntryName","value":"AgrB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006741","properties":[{"key":"EntryName","value":"AgrB"}]},{"database":"Pfam","id":"PF04647","properties":[{"key":"EntryName","value":"AgrB"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00793","properties":[{"key":"EntryName","value":"AgrB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLLIDNGIEKMALKLQQRQNLSHIEFLKVRLGMQVVVINSFKAVVTYGLALLLNIFLYTLIVHLTFLALRGYSHGAHAKTSMLCHVQNIASFVVLPWLIVQYDISFQFLLSLSILAAIIVIIYAPAATKKRPIAPGRVKGLKIKSIIVFIILIALAFIIPAPYNRFVVYGVLLQSFTLLPIFSTKEEV","length":188,"molWeight":21064,"crc64":"AB94919C8E14C187","md5":"7D626D3C309F11D6C4261E02FBC7968C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Transmembrane":5},"uniParcId":"UPI0000504D70"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q56CZ9","uniProtkbId":"Q56CZ9_STAIN","entryAudit":{"firstPublicDate":"2005-05-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-05-10","entryVersion":33,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78517.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00016530"}],"value":"Delta-hemolysin"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00030319"}],"value":"Delta-toxin"}}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00002655"}],"value":"Lyses erythrocytes and many other mammalian cells"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004165"}],"value":"Host cell membrane","id":"SL-0375"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011078"}],"value":"Belongs to the delta-lysin family"}],"commentType":"SIMILARITY"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022852"}],"id":"KW-0204","category":"Biological process","name":"Cytolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023020"}],"id":"KW-0291","category":"PTM","name":"Formylation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022735"}],"id":"KW-0354","category":"Biological process","name":"Hemolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022511"}],"id":"KW-1032","category":"Cellular component","name":"Host cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022870"}],"id":"KW-1043","category":"Cellular component","name":"Host membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022656"}],"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023026"}],"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"16622233","citationType":"journal article","authors":["Sung J.M.","Chantler P.D.","Lloyd D.H."],"citationCrossReferences":[{"database":"PubMed","id":"16622233"},{"database":"DOI","id":"10.1128/IAI.74.5.2947-2956.2006"}],"title":"Accessory gene regulator locus of Staphylococcus intermedius.","publicationDate":"2006","journal":"Infect. Immun.","firstPage":"2947","lastPage":"2956","volume":"74"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78517.1"}],"value":"S3","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAX78517.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY965912","properties":[{"key":"ProteinId","value":"AAX78517.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q56CZ9","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"1.C.122.1.2","properties":[{"key":"FamilyName","value":"the pore-forming delta-hemolysin (hld) family"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0020002","properties":[{"key":"GoTerm","value":"C:host cell plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0019836","properties":[{"key":"GoTerm","value":"P:symbiont-mediated hemolysis of host erythrocyte"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"InterPro","id":"IPR008034","properties":[{"key":"EntryName","value":"Delta_lysin"}]},{"database":"Pfam","id":"PF05372","properties":[{"key":"EntryName","value":"Delta_lysin"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAADIISTIVEFVKLIAETVAKFIK","length":25,"molWeight":2751,"crc64":"D77A2858ED476C48","md5":"038238583E6CA9807763D43DC377C786"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"uniParcId":"UPI0000504D6F"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q5I2D3","uniProtkbId":"Q5I2D3_STAIN","entryAudit":{"firstPublicDate":"2005-02-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-02-15","entryVersion":34,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAW55662.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00016530"}],"value":"Delta-hemolysin"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00030319"}],"value":"Delta-toxin"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAW55662.1"}],"value":"hld"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00002655"}],"value":"Lyses erythrocytes and many other mammalian cells"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004165"}],"value":"Host cell membrane","id":"SL-0375"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00011078"}],"value":"Belongs to the delta-lysin family"}],"commentType":"SIMILARITY"}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022852"}],"id":"KW-0204","category":"Biological process","name":"Cytolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023020"}],"id":"KW-0291","category":"PTM","name":"Formylation"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022735"}],"id":"KW-0354","category":"Biological process","name":"Hemolysis"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022511"}],"id":"KW-1032","category":"Cellular component","name":"Host cell membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022870"}],"id":"KW-1043","category":"Cellular component","name":"Host membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023136"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022656"}],"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022692"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023026"}],"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"15838041","citationType":"journal article","authors":["Ji G.","Pei W.","Zhang L.","Qiu R.","Lin J.","Benito Y.","Lina G.","Novick R.P."],"citationCrossReferences":[{"database":"PubMed","id":"15838041"},{"database":"DOI","id":"10.1128/JB.187.9.3139-3150.2005"}],"title":"Staphylococcus intermedius produces a functional agr autoinducing peptide containing a cyclic lactone.","publicationDate":"2005","journal":"J. Bacteriol.","firstPage":"3139","lastPage":"3150","volume":"187"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAW55662.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY860843","properties":[{"key":"ProteinId","value":"AAW55662.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_103265969.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_JBBLTU010000007.1"}]},{"database":"AlphaFoldDB","id":"Q5I2D3","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0020002","properties":[{"key":"GoTerm","value":"C:host cell plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0019836","properties":[{"key":"GoTerm","value":"P:symbiont-mediated hemolysis of host erythrocyte"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"InterPro","id":"IPR008034","properties":[{"key":"EntryName","value":"Delta_lysin"}]},{"database":"Pfam","id":"PF05372","properties":[{"key":"EntryName","value":"Delta_lysin"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAGDIISTIVDFIKLIAETVKKFTK","length":25,"molWeight":2782,"crc64":"E16BD858FB0469D6","md5":"08D31F9265077D08296677911E03EE9C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"uniParcId":"UPI00004C1D83"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q71T46","uniProtkbId":"Q71T46_STAIN","entryAudit":{"firstPublicDate":"2004-07-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":77,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAG09762.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00016505"}],"value":"rRNA adenine N-6-methyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012304"}],"value":"2.1.1.184"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00030809"}],"value":"Erythromycin resistance protein"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00029941"}],"value":"Macrolide-lincosamide-streptogramin B resistance protein"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAG09762.1"}],"value":"ermB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003100"}],"value":"This protein produces a dimethylation of the adenine residue at position 2085 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"adenosine(2085) in 23S rRNA + 2 S-adenosyl-L-methionine = N(6)-dimethyladenosine(2085) in 23S rRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42784"},{"database":"Rhea","id":"RHEA-COMP:10237"},{"database":"Rhea","id":"RHEA-COMP:10238"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:74411"},{"database":"ChEBI","id":"CHEBI:74493"}],"ecNumber":"2.1.1.184","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049167"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"value":"Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"Ribosomal RNA adenine methylase transferase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"SMART","id":"SM00650"}]},{"type":"Binding site","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":12,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022603"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022884"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022691"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"11230937","citationType":"journal article","authors":["Boerlin P.","Burnens A.P.","Frey J.","Kuhnert P.","Nicolet J."],"citationCrossReferences":[{"database":"PubMed","id":"11230937"},{"database":"DOI","id":"10.1016/S0378-1135(00)00347-3"}],"title":"Molecular epidemiology and genetic linkage of macrolide and aminoglycoside resistance in Staphylococcus intermedius of canine origin.","publicationDate":"2001","journal":"Vet. Microbiol.","firstPage":"155","lastPage":"169","volume":"79"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAG09762.1"}],"value":"MLS-17","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAG09762.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF239773","properties":[{"key":"ProteinId","value":"AAG09762.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q71T46","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q71T46","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0052910","properties":[{"key":"GoTerm","value":"F:23S rRNA (adenine(2085)-N(6))-dimethyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0003723","properties":[{"key":"GoTerm","value":"F:RNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000179","properties":[{"key":"GoTerm","value":"F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0031167","properties":[{"key":"GoTerm","value":"P:rRNA methylation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd02440","properties":[{"key":"EntryName","value":"AdoMet_MTases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.8.100","properties":[{"key":"EntryName","value":"Ribosomal RNA adenine dimethylase-like, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.150","properties":[{"key":"EntryName","value":"Vaccinia Virus protein VP39"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001737","properties":[{"key":"EntryName","value":"KsgA/Erm"}]},{"database":"InterPro","id":"IPR023165","properties":[{"key":"EntryName","value":"rRNA_Ade_diMease-like_C"}]},{"database":"InterPro","id":"IPR020596","properties":[{"key":"EntryName","value":"rRNA_Ade_Mease_Trfase_CS"}]},{"database":"InterPro","id":"IPR020598","properties":[{"key":"EntryName","value":"rRNA_Ade_methylase_Trfase_N"}]},{"database":"InterPro","id":"IPR029063","properties":[{"key":"EntryName","value":"SAM-dependent_MTases_sf"}]},{"database":"NCBIfam","id":"NF000499","properties":[{"key":"EntryName","value":"Erm23S_rRNA_broad"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF012220","properties":[{"key":"EntryName","value":"erm_B_23S_MT"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11727","properties":[{"key":"EntryName","value":"DIMETHYLADENOSINE TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11727:SF7","properties":[{"key":"EntryName","value":"DIMETHYLADENOSINE TRANSFERASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00398","properties":[{"key":"EntryName","value":"RrnaAD"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00650","properties":[{"key":"EntryName","value":"rADc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53335","properties":[{"key":"EntryName","value":"S-adenosyl-L-methionine-dependent methyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01131","properties":[{"key":"EntryName","value":"RRNA_A_DIMETH"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51689","properties":[{"key":"EntryName","value":"SAM_RNA_A_N6_MT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK","length":245,"molWeight":28756,"crc64":"4ABC65D91EFF097C","md5":"51D46183EC2AF38C74F5704FBCC15480"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Binding site":6},"uniParcId":"UPI0000000CFD"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q7BLU5","uniProtkbId":"Q7BLU5_STAIN","entryAudit":{"firstPublicDate":"2004-07-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":89,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAG09761.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00016505"}],"value":"rRNA adenine N-6-methyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012304"}],"value":"2.1.1.184"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00030809"}],"value":"Erythromycin resistance protein"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00029941"}],"value":"Macrolide-lincosamide-streptogramin B resistance protein"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAG09761.1"}],"value":"ermB"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAG42227.1"}],"value":"erm(B)"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00003100"}],"value":"This protein produces a dimethylation of the adenine residue at position 2085 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"adenosine(2085) in 23S rRNA + 2 S-adenosyl-L-methionine = N(6)-dimethyladenosine(2085) in 23S rRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42784"},{"database":"Rhea","id":"RHEA-COMP:10237"},{"database":"Rhea","id":"RHEA-COMP:10238"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:74411"},{"database":"ChEBI","id":"CHEBI:74493"}],"ecNumber":"2.1.1.184","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00049167"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"value":"Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"Ribosomal RNA adenine methylase transferase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"SMART","id":"SM00650"}]},{"type":"Binding site","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":12,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022603"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022884"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022691"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01026"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"11230937","citationType":"journal article","authors":["Boerlin P.","Burnens A.P.","Frey J.","Kuhnert P.","Nicolet J."],"citationCrossReferences":[{"database":"PubMed","id":"11230937"},{"database":"DOI","id":"10.1016/S0378-1135(00)00347-3"}],"title":"Molecular epidemiology and genetic linkage of macrolide and aminoglycoside resistance in Staphylococcus intermedius of canine origin.","publicationDate":"2001","journal":"Vet. Microbiol.","firstPage":"155","lastPage":"169","volume":"79"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAG09761.1"}],"value":"MLS-2","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAG09761.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF239772","properties":[{"key":"ProteinId","value":"AAG09761.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AF299292","properties":[{"key":"ProteinId","value":"AAG42227.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_001038792.1","properties":[{"key":"NucleotideSequenceId","value":"NG_047802.1"}]},{"database":"AlphaFoldDB","id":"Q7BLU5","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q7BLU5","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"97507650","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0052910","properties":[{"key":"GoTerm","value":"F:23S rRNA (adenine(2085)-N(6))-dimethyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0003723","properties":[{"key":"GoTerm","value":"F:RNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000179","properties":[{"key":"GoTerm","value":"F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0031167","properties":[{"key":"GoTerm","value":"P:rRNA methylation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd02440","properties":[{"key":"EntryName","value":"AdoMet_MTases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.8.100","properties":[{"key":"EntryName","value":"Ribosomal RNA adenine dimethylase-like, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.150","properties":[{"key":"EntryName","value":"Vaccinia Virus protein VP39"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001737","properties":[{"key":"EntryName","value":"KsgA/Erm"}]},{"database":"InterPro","id":"IPR023165","properties":[{"key":"EntryName","value":"rRNA_Ade_diMease-like_C"}]},{"database":"InterPro","id":"IPR020596","properties":[{"key":"EntryName","value":"rRNA_Ade_Mease_Trfase_CS"}]},{"database":"InterPro","id":"IPR020598","properties":[{"key":"EntryName","value":"rRNA_Ade_methylase_Trfase_N"}]},{"database":"InterPro","id":"IPR029063","properties":[{"key":"EntryName","value":"SAM-dependent_MTases_sf"}]},{"database":"NCBIfam","id":"NF000499","properties":[{"key":"EntryName","value":"Erm23S_rRNA_broad"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF012220","properties":[{"key":"EntryName","value":"erm_B_23S_MT"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11727","properties":[{"key":"EntryName","value":"DIMETHYLADENOSINE TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11727:SF7","properties":[{"key":"EntryName","value":"DIMETHYLADENOSINE TRANSFERASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00398","properties":[{"key":"EntryName","value":"RrnaAD"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00650","properties":[{"key":"EntryName","value":"rADc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53335","properties":[{"key":"EntryName","value":"S-adenosyl-L-methionine-dependent methyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01131","properties":[{"key":"EntryName","value":"RRNA_A_DIMETH"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51689","properties":[{"key":"EntryName","value":"SAM_RNA_A_N6_MT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK","length":245,"molWeight":28768,"crc64":"5E3AB1ACC6B511C5","md5":"E61FC7C0F24C07720DD3A7778D6FE264"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SIMILARITY":1},"countByFeatureType":{"Domain":1,"Binding site":6},"uniParcId":"UPI00000037E2"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q8VKX4","uniProtkbId":"Q8VKX4_STAIN","entryAudit":{"firstPublicDate":"2002-03-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-03-01","entryVersion":76,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAC86489.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012682"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"value":"Superoxide dismutase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00012682"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"value":"1.15.1.1"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAC86489.1"}],"value":"sodA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"value":"Destroys radicals which are normally produced within the cells and which are toxic to biological systems"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 superoxide + 2 H(+) = H2O2 + O2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:20696"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16240"},{"database":"ChEBI","id":"CHEBI:18421"}],"ecNumber":"1.15.1.1","evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001936"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00008714"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"value":"Belongs to the iron/manganese superoxide dismutase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"Manganese/iron superoxide dismutase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00081"}]},{"type":"Domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":142,"modifier":"EXACT"}},"description":"Manganese/iron superoxide dismutase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02777"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAC86489.1"}]},{"type":"Non-terminal residue","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAC86489.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023211"}],"id":"KW-0464","category":"Ligand","name":"Manganese"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023002"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000414"}],"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"11724835","citationType":"journal article","authors":["Poyart C.","Quesne G.","Boumaila C.","Trieu-Cuot P."],"citationCrossReferences":[{"database":"PubMed","id":"11724835"},{"database":"DOI","id":"10.1128/JCM.39.12.4296-4301.2001"}],"title":"Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target.","publicationDate":"2001","journal":"J. Clin. Microbiol.","firstPage":"4296","lastPage":"4301","volume":"39"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAC86489.1"}],"value":"CIP 81.60 T","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"CAC86489.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ343914","properties":[{"key":"ProteinId","value":"CAC86489.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q8VKX4","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004784","properties":[{"key":"GoTerm","value":"F:superoxide dismutase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"FunFam","id":"1.10.287.990:FF:000001","properties":[{"key":"EntryName","value":"Superoxide dismutase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.287.990","properties":[{"key":"EntryName","value":"Fe,Mn superoxide dismutase (SOD) domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.55.40.20","properties":[{"key":"EntryName","value":"Iron/manganese superoxide dismutase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001189","properties":[{"key":"EntryName","value":"Mn/Fe_SOD"}]},{"database":"InterPro","id":"IPR019832","properties":[{"key":"EntryName","value":"Mn/Fe_SOD_C"}]},{"database":"InterPro","id":"IPR019831","properties":[{"key":"EntryName","value":"Mn/Fe_SOD_N"}]},{"database":"InterPro","id":"IPR036324","properties":[{"key":"EntryName","value":"Mn/Fe_SOD_N_sf"}]},{"database":"InterPro","id":"IPR036314","properties":[{"key":"EntryName","value":"SOD_C_sf"}]},{"database":"PANTHER","id":"PTHR43595","properties":[{"key":"EntryName","value":"37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43595:SF2","properties":[{"key":"EntryName","value":"SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02777","properties":[{"key":"EntryName","value":"Sod_Fe_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00081","properties":[{"key":"EntryName","value":"Sod_Fe_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01703","properties":[{"key":"EntryName","value":"MNSODISMTASE"}]},{"database":"SUPFAM","id":"SSF54719","properties":[{"key":"EntryName","value":"Fe,Mn superoxide dismutase (SOD), C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF46609","properties":[{"key":"EntryName","value":"Fe,Mn superoxide dismutase (SOD), N-terminal domain"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"HIDKETMEIHHSKHHNTYVTKLNAAVEGTEFENKSLEDLIANLNSVPENIRTAVRNNGGGHLNHSIFWQLLTPNSEEKGEVVDKIKEQWGSLDEFKNEFADKAAARFGSGWAWLVVNNGKLEIVTTANQDSPLTDGLSPILAL","length":143,"molWeight":15915,"crc64":"CEF912B584AA8249","md5":"8D6E2AC1AAE07B564125036AB3AE8197"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SIMILARITY":1},"countByFeatureType":{"Domain":2,"Non-terminal residue":2},"uniParcId":"UPI00000B5007"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"Q8VTK9","uniProtkbId":"Q8VTK9_STAIN","entryAudit":{"firstPublicDate":"2002-03-01","lastAnnotationUpdateDate":"2026-11-04","lastSequenceUpdateDate":"2002-03-01","entryVersion":101,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Staphylococcus intermedius","taxonId":1285,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL37307.1"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus","Staphylococcus intermedius group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"value":"DNA-directed RNA polymerase subunit beta"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"value":"2.7.7.6"}]},"flag":"Fragment"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL37307.1"}],"value":"rpoB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004026"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"value":"DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21248"},{"database":"Rhea","id":"RHEA-COMP:14527"},{"database":"Rhea","id":"RHEA-COMP:17342"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:61557"},{"database":"ChEBI","id":"CHEBI:140395"}],"ecNumber":"2.7.7.6","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00048552"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"value":"The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000434"}],"value":"Belongs to the RNA polymerase beta chain family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Domain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":426,"modifier":"EXACT"}},"description":"RNA polymerase beta subunit protrusion","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04563"}]},{"type":"Domain","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":262,"modifier":"EXACT"}},"description":"RNA polymerase Rpb2","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04561"}]},{"type":"Domain","location":{"start":{"value":346,"modifier":"EXACT"},"end":{"value":382,"modifier":"EXACT"}},"description":"RNA polymerase Rpb2","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04561"}]},{"type":"Domain","location":{"start":{"value":441,"modifier":"EXACT"},"end":{"value":509,"modifier":"EXACT"}},"description":"RNA polymerase Rpb2","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04565"}]},{"type":"Domain","location":{"start":{"value":519,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"DNA-directed RNA polymerase beta subunit external 1","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF10385"}]},{"type":"Domain","location":{"start":{"value":648,"modifier":"EXACT"},"end":{"value":1042,"modifier":"EXACT"}},"description":"DNA-directed RNA polymerase subunit 2 hybrid-binding","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF00562"}]},{"type":"Domain","location":{"start":{"value":1044,"modifier":"EXACT"},"end":{"value":1119,"modifier":"EXACT"}},"description":"RNA polymerase Rpb2","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF04560"}]},{"type":"Region","location":{"start":{"value":1126,"modifier":"EXACT"},"end":{"value":1156,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1126,"modifier":"EXACT"},"end":{"value":1135,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL37307.1"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022478"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"id":"KW-0240","category":"Cellular component","name":"DNA-directed RNA polymerase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022695"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023163"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"id":"KW-0804","category":"Biological process","name":"Transcription"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022679"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU363031"}],"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"11923353","citationType":"journal article","authors":["Drancourt M.","Raoult D."],"citationCrossReferences":[{"database":"PubMed","id":"11923353"},{"database":"DOI","id":"10.1128/JCM.40.4.1333-1338.2002"}],"title":"rpoB gene sequence-based identification of Staphylococcus species.","publicationDate":"2002","journal":"J. Clin. Microbiol.","firstPage":"1333","lastPage":"1338","volume":"40"},"referencePositions":["NUCLEOTIDE SEQUENCE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL37307.1"}],"value":"Institut Pasteur CIP8160 T","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"AAL37307.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF325869","properties":[{"key":"ProteinId","value":"AAL37307.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003899","properties":[{"key":"GoTerm","value":"F:DNA-directed RNA polymerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0032549","properties":[{"key":"GoTerm","value":"F:ribonucleoside binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006351","properties":[{"key":"GoTerm","value":"P:DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd00653","properties":[{"key":"EntryName","value":"RNA_pol_B_RPB2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.1800.10:FF:000001","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase subunit beta"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.100","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.150","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.1100.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"3"}]},{"database":"Gene3D","id":"2.40.270.10","properties":[{"key":"EntryName","value":"DNA-directed RNA polymerase, subunit 2, domain 6"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.1800.10","properties":[{"key":"EntryName","value":"RNA polymerase alpha subunit dimerisation domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.1110.10","properties":[{"key":"EntryName","value":"RNA polymerase Rpb2, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01321","properties":[{"key":"EntryName","value":"RNApol_bact_RpoB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR019462","properties":[{"key":"EntryName","value":"DNA-dir_RNA_pol_bsu_external_1"}]},{"database":"InterPro","id":"IPR015712","properties":[{"key":"EntryName","value":"DNA-dir_RNA_pol_su2"}]},{"database":"InterPro","id":"IPR007120","properties":[{"key":"EntryName","value":"DNA-dir_RNAP_su2_dom"}]},{"database":"InterPro","id":"IPR037033","properties":[{"key":"EntryName","value":"DNA-dir_RNAP_su2_hyb_sf"}]},{"database":"InterPro","id":"IPR010243","properties":[{"key":"EntryName","value":"RNA_pol_bsu_bac"}]},{"database":"InterPro","id":"IPR007121","properties":[{"key":"EntryName","value":"RNA_pol_bsu_CS"}]},{"database":"InterPro","id":"IPR007644","properties":[{"key":"EntryName","value":"RNA_pol_bsu_protrusion"}]},{"database":"InterPro","id":"IPR007642","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_2"}]},{"database":"InterPro","id":"IPR037034","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_2_sf"}]},{"database":"InterPro","id":"IPR007645","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_3"}]},{"database":"InterPro","id":"IPR007641","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_7"}]},{"database":"InterPro","id":"IPR014724","properties":[{"key":"EntryName","value":"RNA_pol_RPB2_OB-fold"}]},{"database":"NCBIfam","id":"NF001616","properties":[{"key":"EntryName","value":"rpoB"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR02013","properties":[{"key":"EntryName","value":"rpoB"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR20856","properties":[{"key":"EntryName","value":"DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04563","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04561","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_2"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF04565","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF10385","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_45"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00562","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_6"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04560","properties":[{"key":"EntryName","value":"RNA_pol_Rpb2_7"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF64484","properties":[{"key":"EntryName","value":"beta and beta-prime subunits of DNA dependent RNA-polymerase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01166","properties":[{"key":"EntryName","value":"RNA_POL_BETA"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"NLIEIXTKSYDWFLKEGLLEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKETGEVKDQEVFMGDFPLMTETGTFVINGAERVIVSQLVRSPSVYFNEKLDKNGCVNYDATVIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGYSTDQEIIELIGDNEYLRNTLEKDSTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKANKKLHLKHRLFNQKLAEPIVNTETGEIVAEEGTVLDRRKLDEIMDVLETNANAQVYEHSKRIIDEPVEIQSIKVYVPNDDEERTTTVIGNAFPDSEVKCITPADIVASMSYFFNLLHGIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTDSITPQQLINIRPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYSHYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDIETNTITDQIDYLTADEEDSYVVAQANSRLDENGRFIDDEIVCRFRGNNTTMAKEKMDYMDVSPKQVVSAATACIPFLENDDSNRALMGANMQRQAVPLLNPESPFVGTGMEHVAARDSGAAVISKYRGRVEHVQSSEILVRRLVEENGQEVDGTLDRYPLAKFKRSNSGTCYNQRPIIAKGDIVEKGEILADGPSMELGEMALGQKRSSWFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEARDTKLGPEEITRDIPNVSENALKNLDDRGIVYVGAEVKDGDILVGKVTPKGVTELTAEERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDSLSPGVNQLVRVYIVQKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVLELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRISVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMDEQDNEIEMRDLDDDDIPDRKVNIQPSTVPESQKEFNE","length":1156,"molWeight":130320,"crc64":"782A56CCA808BDCF","md5":"EA109C2F537AF39F58724CD475085AC9"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Domain":7,"Region":1,"Compositional bias":1,"Non-terminal residue":1},"uniParcId":"UPI00000BDDE8"}}]}