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Therefore, by controlling the phosphorylation state of HPr and crh, HPrK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"[HPr protein]-L-serine + ATP = [HPr protein]-O-phospho-L-serine + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:46600"},{"database":"Rhea","id":"RHEA-COMP:11602"},{"database":"Rhea","id":"RHEA-COMP:11603"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29999"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:83421"},{"database":"ChEBI","id":"CHEBI:456216"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001120"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"[HPr protein]-O-phospho-L-serine + phosphate + H(+) = [HPr protein]-L-serine + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:46604"},{"database":"Rhea","id":"RHEA-COMP:11602"},{"database":"Rhea","id":"RHEA-COMP:11603"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29999"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:83421"}],"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00047657"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}],"value":"Homohexamer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}],"value":"The Walker A ATP-binding motif also binds Pi and PPi"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}],"value":"Both phosphorylation and phosphorolysis are carried out by the same active site and suggest a common mechanism for both reactions"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00006883"},{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}],"value":"Belongs to the HPrK/P family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"MFD1606225.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Domain","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"HPr(Ser) kinase/phosphorylase N-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF02603"}]},{"type":"Domain","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":298,"modifier":"EXACT"}},"description":"HPr kinase/phosphorylase C-terminal","evidences":[{"evidenceCode":"ECO:0000259","source":"Pfam","id":"PF07475"}]},{"type":"Region","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"Important for the catalytic mechanism of both phosphorylation and dephosphorylation","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}]},{"type":"Region","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":269,"modifier":"EXACT"}},"description":"Important for the catalytic mechanism of dephosphorylation","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}]},{"type":"Active site","location":{"start":{"value":159,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"HAMAP-Rule","id":"MF_01249"}]},{"type":"Active site","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"Proton acceptor; for phosphorylation activity. 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