{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O31506","secondaryAccessions":["O30581"],"uniProtkbId":"YEEF_BACSU","entryAudit":{"firstPublicDate":"2009-01-20","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2009-07-07","entryVersion":110,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Bacillales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34280190"}],"value":"Toxin YeeF"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34280190"}],"value":"DNase YeeF"}}]},"genes":[{"geneName":{"value":"yeeF"},"orderedLocusNames":[{"value":"BSU06810"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"E0TU96"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34280190"}],"value":"Toxic component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. Overexpression alone in situ causes growth arrest but not cell lysis, a large decrease in chromosomal DNA content and the production of anucleate cells. No effect is seen on rRNA. Co-overexpression with cognate immunity protein YezG does not cause growth arrest. The toxic effect is dependent on the epsA and tapA operons which are required for biofilm formation (PubMed:34280190). Binds DNA in the presence and absence of YezG (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22200572"}],"value":"Interacts with cognate immunity protein YezG but not with non-cognate immunity protein YobK. The interaction probably inhibits the toxic activity of YeeF"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22882210"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34280190"}],"value":"Present in detergent-resistant membrane (DRM) fractions that may be equivalent to eukaryotic membrane rafts; these rafts include proteins involved in signaling, molecule trafficking and protein secretion (PubMed:22882210). Delivery to target cells requires the type VII secretion system (T7SS) and YukE (PubMed:34280190)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22882210"}],"value":"Cell membrane","id":"SL-0039"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22882210"}],"value":"Membrane raft","id":"SL-0370"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34280190"}],"value":"Expressed on rich and minimal solid media likely in early stationary phase; dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34280190"}],"value":"Deletion of the yeeF-yezG operon has no visible growth phenotype, however it is out-competed by wild-type cells"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22200572"}],"value":"In the N-terminal section; belongs to the LXG family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22200572"}],"value":"Was originally suggested to be an RNase"}],"commentType":"CAUTION"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"AAB66479.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":669,"modifier":"EXACT"}},"description":"Toxin YeeF","featureId":"PRO_0000360798"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"LXG","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01092"}]},{"type":"Region","location":{"start":{"value":561,"modifier":"EXACT"},"end":{"value":580,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled coil","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Coiled coil","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Coiled coil","location":{"start":{"value":226,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Compositional bias","location":{"start":{"value":570,"modifier":"EXACT"},"end":{"value":580,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Sequence conflict","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"in Ref. 1; 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Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, inducing cannibalism to provide a source of nutrients to support sporulation, and at the same time delaying commitment to the energetically expensive and irreversible onset of sporulation (PubMed:12817086). Addition of SDP to liquid cultures halts growth, leads to increased cell permeability and eventually cell lysis in a significant subset of the population, although some cells survive and resume growth after a lag period (PubMed:20805502). Effects of SDP are irreversible within 10 minutes (PubMed:22469514). Addition of SDP to solid cultures induces killing, it is much more effective than SKF (AC O31422) (PubMed:20805502). Has antibiotic action against Gram-positive Firmicutes (L.acidophilus, M.megaterium, P.polymyxa, S.aureus, S.epidermidis) but not Actinobacteria M.luteus or Gram-negative P.aeruginosa or K.pneumoniae (PubMed:20805502, PubMed:22469514). SDP induces expression of the sdpR-sdpI operon (PubMed:12817086). Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, binds to SdpI triggering a signal transduction cascade that protects the cell against the toxic effects of its own SDP"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"13129613"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"10816431"}],"value":"Proprotein probably interacts with chaperone CsaA"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Produces a secreted protein originating from this gene (PubMed:12817086), secreted by the general secretory pathway (PubMed:14568161)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12817086"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14568161"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23687264"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12817086"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14568161"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17720793"}],"value":"By Spo0A during nutrient starvation, through its direct negative control of AbrB (PubMed:12817086). 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The ntnh-pmp1 construct is about half as toxic. PMP1 is toxic when injected directly into Anopheles or Aedes mosquito 3rd instar larvae, larvae no longer move, suggesting they are paralyzed. Adult mosquitoes (Anopheles or Aedes) and Drosophila lose the ability to fly in a dose-dependent manner by 24 hours after injection with 100 pg neurotoxin. Not toxic upon injection in mice"}],"commentType":"FUNCTION","molecule":"Paraclostridial mosquitocidal protein 1"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31253776"}],"value":"Neurotoxin that cleaves A.gambiae syntaxin 1a, probably hydrolyzing the '240-Glu-|-His-241' bond. 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Antibodies were raised against a peptide in the heavy chain (residues 422-435)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31253776"}],"value":"Has protease activity which is probably neurotoxic"}],"commentType":"DOMAIN","molecule":"Paraclostridial mosquitocidal protein 1 light chain"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DPI0"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31253776"}],"value":"Has 3 functional domains; the translocation domain (TD) and the receptor-binding domain (RBD) which is further subdivided into N- and C-terminal domains (HCN and HCC) (By similarity). HCN has a lectin-like fold, HCC is the main region associated with cell recognition. Probably recognizes host cells via other residues than Clostrial botulinum-type toxins, as conserved residues important for ganglioside recognition can be mutated with little effect (PubMed:31253776)"}],"commentType":"DOMAIN","molecule":"Paraclostridial mosquitocidal protein 1 heavy chain"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31253776"}],"value":"LD(50) is 14 pg (98 amol) per Aedes 3rd instar larve when injected directly between the head and thorax"}],"commentType":"TOXIC DOSE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31253776"}],"value":"LD(50) is 6.5 pg (44.5 amol) per Anopheles 3rd instar larve when injected directly between the head and thorax"}],"commentType":"TOXIC DOSE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31253776"}],"value":"Identification of mosquitocidal P.bifermentans strains in regions where mosquito-borne diseases are endemic could help control mosquito populations. 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