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Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT) (PubMed:22385435)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"thymidine + ATP = dTMP + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19129"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17748"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:63528"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.1.21","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14697231"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22385435"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:19130"},"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"14697231"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22385435"}]}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":26.6,"unit":"umol/min/mg","enzyme":"enzyme toward thymidine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22385435"}]},{"velocity":10.0,"unit":"umol/min/mg","enzyme":"enzyme toward AZT","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22385435"}]}],"michaelisConstants":[{"constant":1.4,"unit":"uM","substrate":"thymidine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22385435"}]},{"constant":0.54,"unit":"uM","substrate":"thymidine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14697231"}]},{"constant":0.52,"unit":"uM","substrate":"AZT","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22385435"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14697231"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22385435"}],"value":"Kcats are 9.5 sec(-1) and 3.5 sec(-1) with thymidine and AZT as substrates, respectively (PubMed:22385435). 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